ATE478158T1 - Hybridisierungsverfahren - Google Patents
HybridisierungsverfahrenInfo
- Publication number
- ATE478158T1 ATE478158T1 AT05737054T AT05737054T ATE478158T1 AT E478158 T1 ATE478158 T1 AT E478158T1 AT 05737054 T AT05737054 T AT 05737054T AT 05737054 T AT05737054 T AT 05737054T AT E478158 T1 ATE478158 T1 AT E478158T1
- Authority
- AT
- Austria
- Prior art keywords
- hybridization
- reaction
- target gene
- temperature region
- hybridization process
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 7
- 238000009396 hybridization Methods 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 230000035945 sensitivity Effects 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/54—Heating or cooling apparatus; Heat insulating devices using spatial temperature gradients
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/125—Sandwich assay format
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/519—Detection characterised by immobilisation to a surface characterised by the capture moiety being a single stranded oligonucleotide
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Bipolar Transistors (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004133870 | 2004-04-28 | ||
| PCT/JP2005/008248 WO2005106031A1 (ja) | 2004-04-28 | 2005-04-28 | ハイブリダイゼーション方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE478158T1 true ATE478158T1 (de) | 2010-09-15 |
Family
ID=35241686
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05737054T ATE478158T1 (de) | 2004-04-28 | 2005-04-28 | Hybridisierungsverfahren |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7745124B2 (de) |
| EP (1) | EP1741793B1 (de) |
| JP (1) | JP4482557B2 (de) |
| KR (1) | KR101146251B1 (de) |
| CN (1) | CN100547080C (de) |
| AT (1) | ATE478158T1 (de) |
| AU (1) | AU2005238378B2 (de) |
| CA (1) | CA2564838A1 (de) |
| DE (1) | DE602005023002D1 (de) |
| WO (1) | WO2005106031A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007108378A1 (ja) | 2006-03-15 | 2007-09-27 | Eisai R & D Management Co., Ltd. | シグナルプローブポリマーの形成方法 |
| EP2180063B1 (de) * | 2007-08-14 | 2013-01-09 | Eisai R&D Management Co., Ltd. | Verfahren zur erkennung einer zielsubstanz |
| WO2009022683A1 (ja) * | 2007-08-14 | 2009-02-19 | Eisai R & D Management Co., Ltd. | 標的物質の検出方法 |
| EP2202319A4 (de) * | 2007-10-24 | 2010-10-20 | Eisai R&D Man Co Ltd | Nukleinsäuresonde und verfahren zur herstellung eines sondenpolymers |
| WO2009057702A1 (ja) | 2007-10-31 | 2009-05-07 | Eisai R & D Management Co., Ltd. | 標的物質検出用ポリマー及び標的物質の検出方法 |
| EP4060047A4 (de) * | 2019-11-14 | 2023-12-13 | Sekisui Medical Co., Ltd. | Verfahren zum detektieren oder quantifizieren von oligonukleotiden |
| WO2022059462A1 (ja) | 2020-09-17 | 2022-03-24 | 横河電機株式会社 | 核酸配列計測方法、および核酸配列計測用キット |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5175270A (en) * | 1986-09-10 | 1992-12-29 | Polyprobe, Inc. | Reagents for detecting and assaying nucleic acid sequences |
| CA2039517C (en) * | 1990-04-03 | 2006-11-07 | David Segev | Dna probe signal amplification |
| DK51092D0 (da) | 1991-05-24 | 1992-04-15 | Ole Buchardt | Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf |
| JP2002505846A (ja) * | 1997-11-06 | 2002-02-26 | モザイク テクノロジーズ | シグナル増幅およびプロセッシングのための多重逐次的ポリヌクレオチド置換反応 |
| JP3267576B2 (ja) * | 1998-11-09 | 2002-03-18 | 三光純薬株式会社 | プローブ高分子の形成方法 |
| CA2387755C (en) * | 2000-03-31 | 2007-07-03 | Sanko Junyaku Co., Ltd. | Probe for constructing probe polymer, method of constructing probe polymer and utilization thereof |
| EP1188840A3 (de) * | 2000-07-26 | 2003-04-23 | Agilent Technologies, Inc. (a Delaware corporation) | Chemisches Reaktionsverfahren und Vorrichtung |
| US6544477B1 (en) * | 2000-08-01 | 2003-04-08 | Regents Of The University Of Minnesota | Apparatus for generating a temperature gradient |
| KR20020065471A (ko) * | 2000-08-30 | 2002-08-13 | 산코 준야쿠 가부시키가이샤 | 유전자의 검출방법 |
| ES2273892T3 (es) * | 2000-10-11 | 2007-05-16 | Sanko Junyaku Co., Ltd. | Procedimiento de construccion de sondas de autoensamblaje y procedimiento de deteccion de las mismas. |
| JP5248723B2 (ja) | 2001-01-12 | 2013-07-31 | 株式会社アドバンテスト | 多出力任意波形発生器及びミクスドｌｓｉテスタ |
| JP4505776B2 (ja) * | 2001-01-19 | 2010-07-21 | 凸版印刷株式会社 | 遺伝子検出システム、これを備えた遺伝子検出装置、検出方法、並びに遺伝子検出用チップ |
| JP4121757B2 (ja) | 2001-03-21 | 2008-07-23 | 三光純薬株式会社 | オリゴヌクレオチドによる自己集合体の作製方法及び遺伝子の検出方法 |
| KR20040041529A (ko) | 2001-09-28 | 2004-05-17 | 산코 준야쿠 가부시키가이샤 | Ｄｎａ 칩의 시그널 증폭방법 |
| JPWO2003040367A1 (ja) * | 2001-11-08 | 2005-03-03 | 三光純薬株式会社 | 遺伝子増幅反応で合成されたオリゴヌクレオチドによる自己集合体の形成方法、自己集合体及び遺伝子の検出方法 |
| DE10208770A1 (de) * | 2002-02-28 | 2003-09-04 | Febit Ag | Erhöhung der Sensitivität und Spezifität von Hybridisierungsexperimenten mit Nukleinsäure-Chips |
| US20030198962A1 (en) * | 2002-04-18 | 2003-10-23 | Yung-Chiang Chung | Method and apparatus for nucleic acid hybridization |
-
2005
- 2005-04-28 CN CNB2005800116933A patent/CN100547080C/zh not_active Expired - Fee Related
- 2005-04-28 EP EP05737054A patent/EP1741793B1/de not_active Expired - Lifetime
- 2005-04-28 DE DE602005023002T patent/DE602005023002D1/de not_active Expired - Lifetime
- 2005-04-28 CA CA002564838A patent/CA2564838A1/en not_active Abandoned
- 2005-04-28 KR KR1020067019546A patent/KR101146251B1/ko not_active Expired - Fee Related
- 2005-04-28 US US11/579,063 patent/US7745124B2/en not_active Expired - Fee Related
- 2005-04-28 JP JP2006512861A patent/JP4482557B2/ja not_active Expired - Fee Related
- 2005-04-28 AT AT05737054T patent/ATE478158T1/de not_active IP Right Cessation
- 2005-04-28 AU AU2005238378A patent/AU2005238378B2/en not_active Ceased
- 2005-04-28 WO PCT/JP2005/008248 patent/WO2005106031A1/ja not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CN100547080C (zh) | 2009-10-07 |
| US20080248963A1 (en) | 2008-10-09 |
| KR20070006790A (ko) | 2007-01-11 |
| AU2005238378A1 (en) | 2005-11-10 |
| WO2005106031A1 (ja) | 2005-11-10 |
| KR101146251B1 (ko) | 2012-05-16 |
| AU2005238378B2 (en) | 2010-03-11 |
| EP1741793A1 (de) | 2007-01-10 |
| US7745124B2 (en) | 2010-06-29 |
| CA2564838A1 (en) | 2005-11-10 |
| JPWO2005106031A1 (ja) | 2008-03-13 |
| DE602005023002D1 (de) | 2010-09-30 |
| EP1741793B1 (de) | 2010-08-18 |
| JP4482557B2 (ja) | 2010-06-16 |
| CN1942591A (zh) | 2007-04-04 |
| EP1741793A4 (de) | 2008-05-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |