ATE393244T1 - Verfahren zur genotypisierung durch schmelztemperaturabhängigkeit - Google Patents
Verfahren zur genotypisierung durch schmelztemperaturabhängigkeitInfo
- Publication number
- ATE393244T1 ATE393244T1 AT06001513T AT06001513T ATE393244T1 AT E393244 T1 ATE393244 T1 AT E393244T1 AT 06001513 T AT06001513 T AT 06001513T AT 06001513 T AT06001513 T AT 06001513T AT E393244 T1 ATE393244 T1 AT E393244T1
- Authority
- AT
- Austria
- Prior art keywords
- melting temperature
- genotyping
- temperature dependence
- identification
- nucleic acid
- Prior art date
Links
- 238000002844 melting Methods 0.000 title abstract 2
- 230000008018 melting Effects 0.000 title abstract 2
- 238000003205 genotyping method Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000003155 DNA primer Substances 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Inorganic Compounds Of Heavy Metals (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US64794805P | 2005-01-28 | 2005-01-28 | |
| US66018705P | 2005-03-09 | 2005-03-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE393244T1 true ATE393244T1 (de) | 2008-05-15 |
Family
ID=36250864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06001513T ATE393244T1 (de) | 2005-01-28 | 2006-01-25 | Verfahren zur genotypisierung durch schmelztemperaturabhängigkeit |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20060172324A1 (de) |
| EP (1) | EP1686190B1 (de) |
| JP (1) | JP4351217B2 (de) |
| AT (1) | ATE393244T1 (de) |
| CA (1) | CA2532160C (de) |
| DE (1) | DE602006000944T2 (de) |
| ES (1) | ES2304739T3 (de) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2621146A1 (en) * | 2005-09-06 | 2007-03-15 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
| KR101251177B1 (ko) * | 2007-12-26 | 2013-04-08 | 아크레이 가부시키가이샤 | 융해 곡선 해석 방법 및 융해 곡선 해석 장치 |
| EP2314680B1 (de) * | 2008-07-02 | 2014-10-01 | ARKRAY, Inc. | Verfahren zur amplifikation einer zielnukleinsäuresequenz, verfahren zum mutationsnachweis mit dem verfahren und reagentien zur verwendung in den verfahren |
| US20100112565A1 (en) * | 2008-11-03 | 2010-05-06 | Life Technologies Corporation | Methods, kits, and reaction mixtures for high resolution melt genotyping |
| WO2010070366A1 (en) * | 2008-12-15 | 2010-06-24 | Debreceni Egyetem | Cytometric method for the comparative analysis of the length of pcr products and uses of this method |
| SG10201500705YA (en) * | 2009-01-08 | 2015-04-29 | Bio Rad Laboratories | Methods and compositions for improving efficiency of nucleic acids amplification reactions |
| JP5684737B2 (ja) * | 2010-01-21 | 2015-03-18 | アークレイ株式会社 | 標的配列の増幅方法、多型検出方法およびそれに用いる試薬 |
| JP5876478B2 (ja) | 2010-06-18 | 2016-03-02 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| JP5926247B2 (ja) | 2010-06-18 | 2016-05-25 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| US8722380B2 (en) | 2010-06-18 | 2014-05-13 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-mismatch discrimination |
| WO2011157434A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| JP5926246B2 (ja) | 2010-06-18 | 2016-05-25 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| EP2582802B1 (de) | 2010-06-18 | 2016-01-13 | Roche Diagnostics GmbH | Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz |
| WO2011157437A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| EP2582804B1 (de) | 2010-06-18 | 2015-02-25 | Roche Diagnostics GmbH | Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen |
| WO2012110061A1 (en) | 2011-02-15 | 2012-08-23 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| ITTO20110271A1 (it) * | 2011-03-28 | 2012-09-29 | Fabio Gentilini | Metodo per l'identificazione di polimorfismi genetici di base singola. |
| ES2561885T3 (es) | 2011-04-11 | 2016-03-01 | F. Hoffmann-La Roche Ag | ADN polimerasas de actividad mejorada |
| WO2012146251A1 (en) * | 2011-04-29 | 2012-11-01 | Aarhus Universitet | Method for detecting mutations using a three primer system and differently melting amplicons |
| EP2737061B1 (de) | 2011-07-28 | 2015-09-16 | Roche Diagnostics GmbH | Dna-polymerasen mit verbesserter aktivität |
| CA2858264C (en) | 2011-12-08 | 2018-01-02 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
| ES2569723T3 (es) | 2011-12-08 | 2016-05-12 | F. Hoffmann-La Roche Ag | Polimerasas de DNA con actividad mejorada |
| WO2013083262A1 (en) | 2011-12-08 | 2013-06-13 | Roche Diagnostics Gmbh | Dna polymerases with improved activity |
| EP2841598B1 (de) | 2012-04-25 | 2018-03-14 | Ho, Tho Huu | Verfahren für konkurrenzfähige allelspezifische cdna-synthese und differenzielle amplifikation der cdna-produkte |
| AU2013202808B2 (en) | 2012-07-31 | 2014-11-13 | Gen-Probe Incorporated | System and method for performing multiplex thermal melt analysis |
| US10465238B2 (en) * | 2013-12-19 | 2019-11-05 | The Board Of Trustees Of The Leland Stanford Junior University | Quantification of mutant alleles and copy number variation using digital PCR with nonspecific DNA-binding dyes |
| US11015217B2 (en) | 2015-05-29 | 2021-05-25 | Roche Molecular Systems, Inc. | Method of inactivating microbes by citraconic anhydride |
| CN111868238B (zh) | 2018-03-21 | 2024-06-11 | 豪夫迈·罗氏有限公司 | 用于甲基化-dNTPs的效率高的和有效参入的DNA聚合酶 |
| EP3850086A1 (de) | 2018-09-13 | 2021-07-21 | F. Hoffmann-La Roche AG | Mutante dna-polymerase(n) mit verbesserter strangverschiebbarkeit |
| CN114277108B (zh) * | 2021-12-13 | 2023-05-30 | 上海交通大学医学院附属第一人民医院 | 用于snp位点检测的引物探针组合、试剂盒和方法 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| GB9113223D0 (en) * | 1991-06-19 | 1991-08-07 | Oxford Instr Ltd | Energy storage device |
| US5853989A (en) * | 1991-08-27 | 1998-12-29 | Zeneca Limited | Method of characterisation of genomic DNA |
| EP0655090B1 (de) * | 1992-04-27 | 2000-12-27 | The Trustees Of Dartmouth College | Detektion von gensequenzen in biologischen flüssigkeiten |
| US5981176A (en) * | 1992-06-17 | 1999-11-09 | City Of Hope | Method of detecting and discriminating between nucleic acid sequences |
| US5804380A (en) * | 1993-11-12 | 1998-09-08 | Geron Corporation | Telomerase activity assays |
| EP1320628A2 (de) * | 2000-04-05 | 2003-06-25 | Molecular Staging Inc. | Prozess zur alleldiskriminierung unter verwendung von primer verlängerung |
| US6586183B2 (en) * | 2000-04-13 | 2003-07-01 | Genaissance Pharmaceuticals, Inc. | Association of β2-adrenergic receptor haplotypes with drug response |
| AU7125401A (en) * | 2000-05-19 | 2001-12-03 | David J Marshall | Materials and methods for detection of nucleic acids |
| IL144515A0 (en) * | 2000-07-31 | 2002-05-23 | Pfizer Prod Inc | A pcr-based multiplex assay for determining haplotype |
| CA2417986C (en) * | 2000-08-11 | 2013-11-26 | University Of Utah Research Foundation | Single-labeled oligonucleotide probes |
| FI20001839L (fi) * | 2000-08-21 | 2002-02-22 | Timo Korpela | Hemokromatoosimutaation analyysimenetelmiä |
| US20030096277A1 (en) * | 2001-08-30 | 2003-05-22 | Xiangning Chen | Allele specific PCR for genotyping |
| US7153656B2 (en) * | 2001-09-11 | 2006-12-26 | Los Alamos National Security, Llc | Nucleic acid sequence detection using multiplexed oligonucleotide PCR |
| US20040121374A1 (en) * | 2002-10-02 | 2004-06-24 | Yoshihide Iwaki | Method for detecting single nucleotide polymorphisms |
| US20050130213A1 (en) * | 2003-12-10 | 2005-06-16 | Tom Morrison | Selective ligation and amplification assay |
| US7387887B2 (en) * | 2004-04-20 | 2008-06-17 | University Of Utah Research Foundation | Nucleic acid melting analysis with saturation dyes |
| US20060105348A1 (en) * | 2004-11-15 | 2006-05-18 | Lee Jun E | Compositions and methods for the detection and discrimination of nucleic acids |
-
2005
- 2005-12-08 US US11/298,152 patent/US20060172324A1/en not_active Abandoned
-
2006
- 2006-01-25 DE DE602006000944T patent/DE602006000944T2/de active Active
- 2006-01-25 ES ES06001513T patent/ES2304739T3/es active Active
- 2006-01-25 CA CA2532160A patent/CA2532160C/en not_active Expired - Fee Related
- 2006-01-25 EP EP06001513A patent/EP1686190B1/de not_active Not-in-force
- 2006-01-25 AT AT06001513T patent/ATE393244T1/de active
- 2006-01-27 JP JP2006018750A patent/JP4351217B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2532160C (en) | 2011-01-18 |
| DE602006000944T2 (de) | 2009-05-20 |
| DE602006000944D1 (de) | 2008-06-05 |
| JP2006230401A (ja) | 2006-09-07 |
| JP4351217B2 (ja) | 2009-10-28 |
| EP1686190A1 (de) | 2006-08-02 |
| US20060172324A1 (en) | 2006-08-03 |
| ES2304739T3 (es) | 2008-10-16 |
| EP1686190B1 (de) | 2008-04-23 |
| CA2532160A1 (en) | 2006-07-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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