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WO2011115366A3 - 대한민국 연안 규조류의 종 판별 방법과 이에 따른 규조류의 종 판별용 폴리뉴클레오티드 프로브, ｄｎａ 칩 및 키트 - Google Patents

대한민국 연안 규조류의 종 판별 방법과 이에 따른 규조류의 종 판별용 폴리뉴클레오티드 프로브, ｄｎａ 칩 및 키트 Download PDF

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Publication number
WO2011115366A3
WO2011115366A3 PCT/KR2011/000592 KR2011000592W WO2011115366A3 WO 2011115366 A3 WO2011115366 A3 WO 2011115366A3 KR 2011000592 W KR2011000592 W KR 2011000592W WO 2011115366 A3 WO2011115366 A3 WO 2011115366A3
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WIPO (PCT)
Prior art keywords
diatoms
species
identification
coast
korea
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Ceased
Application number
PCT/KR2011/000592
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English (en)
French (fr)
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WO2011115366A2 (ko
Inventor
이택견
박소윤
황진익
이윤호
장만
이건섭
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Korea Ocean Research and Development Institute (KORDI)
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Korea Ocean Research and Development Institute (KORDI)
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Priority to US13/635,887 priority Critical patent/US20130123125A1/en
Publication of WO2011115366A2 publication Critical patent/WO2011115366A2/ko
Publication of WO2011115366A3 publication Critical patent/WO2011115366A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 한국 연안에 서식하는 규조류의 종(species)을 판별하는 방법과, 이를 위한 규조류의 종 판별용 폴리뉴클레오티드 프로브, DNA 칩 및 키트에 대한 것으로, 규조류에서 추출한 DNA에 대해 중합효소연쇄반응(PCR)을 수행하여 PCR 산물을 얻는 단계; 상기 PCR 산물을, 서열번호 3 내지 서열번호 51 중 하나 이상의 각 DNA 서열과 동일하거나 그에 상보적인(complementary) 염기서열을 각각 포함하는 프로브에 결합시키는 단계; 및, 상기 결합 여부에 따라 상기 규조류가 속하는 종을 판별하는 단계;를 포함하는 것이 특징이다. 이러한 본 발명은 다양한 규조류 종의 단염기다형성(single nucleotide polymorphism, SNPs)을 기반으로, 한국 연안에 서식하는 규조류에 대한 유전자형을 분석하고, 간단하고 신속, 정확하게 그것의 종을 판별할 수 있는 효과가 있다.
PCT/KR2011/000592 2010-03-19 2011-01-28 대한민국 연안 규조류의 종 판별 방법과 이에 따른 규조류의 종 판별용 폴리뉴클레오티드 프로브, ｄｎａ 칩 및 키트 Ceased WO2011115366A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/635,887 US20130123125A1 (en) 2010-03-19 2011-01-28 Identification Method of Species of Diatoms in Coast of Korea, and Polynucleotide Probe, DNA Chip and Kit for Identification of Species of Diatoms According to Same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100024939A KR101403351B1 (ko) 2010-03-19 2010-03-19 대한민국 연안 규조류의 종 판별 방법과 이에 따른 규조류의 종 판별용 폴리뉴클레오티드 프로브, ｄｎａ 칩 및 키트
KR10-2010-0024939 2010-03-19

Publications (2)

Publication Number Publication Date
WO2011115366A2 WO2011115366A2 (ko) 2011-09-22
WO2011115366A3 true WO2011115366A3 (ko) 2012-03-29

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ID=44649684

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PCT/KR2011/000592 Ceased WO2011115366A2 (ko) 2010-03-19 2011-01-28 대한민국 연안 규조류의 종 판별 방법과 이에 따른 규조류의 종 판별용 폴리뉴클레오티드 프로브, ｄｎａ 칩 및 키트

Country Status (3)

Country Link
US (1) US20130123125A1 (ko)
KR (1) KR101403351B1 (ko)
WO (1) WO2011115366A2 (ko)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102433390B (zh) * 2012-01-09 2013-08-07 上海海洋大学 一种检测利玛原甲藻的方法
KR101687268B1 (ko) * 2014-06-23 2016-12-19 한국해양과학기술원 대한민국 연안 미세조류인 디틸럼 브라이트웰리의 종 판별 방법과 이에 따른 디틸럼 브라이트웰리의 종 판별용 폴리뉴클레오티드 프로브, ｄｎａ 칩 및 키트
KR101603755B1 (ko) * 2014-06-23 2016-03-18 한국해양과학기술원 대한민국 연안 미세조류인 헤테로시그마 아카쉬요의 종 판별 방법과 이에 따른 헤테로시그마 아카쉬요의 종 판별용 폴리뉴클레오티드 프로브, ｄｎａ 칩 및 키트
KR101602349B1 (ko) * 2014-06-23 2016-03-14 한국해양과학기술원 대한민국 연안 미세조류인 아카쉬요 산구이니아의 종 판별 방법과 이에 따른아카쉬요 산구이니아의 종 판별용 폴리뉴클레오티드 프로브, ｄｎａ 칩 및 키트
KR101599427B1 (ko) * 2014-06-23 2016-03-04 한국해양과학기술원 대한민국 연안 미세조류인 니츠치아 임프로비사의 종 판별 방법과 이에 따른 니츠치아 임프로비사의 종 판별용 폴리뉴클레오티드 프로브, ｄｎａ 칩 및 키트
KR101602346B1 (ko) * 2014-06-23 2016-03-14 한국해양과학기술원 대한민국 연안 미세조류인 프로로센트럼 미니멈의 종 판별 방법과 이에 따른 프로로센트럼 미니멈의 종 판별용 폴리뉴클레오티드 프로브, ｄｎａ 칩 및 키트
KR101706771B1 (ko) 2015-06-12 2017-02-17 한국해양과학기술원 인시츄 혼성화를 사용하는 코클로디니움 폴리클리코이데스 검출방법 및 검출용 키트
CN106701927B (zh) * 2016-12-09 2020-09-29 国投生物科技投资有限公司 一种环介导等温扩增快速检测金藻的方法及试剂盒
CN118910322B (zh) * 2024-09-24 2025-05-02 深圳市环境科学研究院 一种检测常见真核藻的探针组合物、基因芯片、试剂盒及方法

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US5958689A (en) * 1996-05-22 1999-09-28 Monterey Bay Aquarium Research Institute Detection of toxigenic marine diatoms of the genus Pseudo-nitzschia

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KR100774367B1 (ko) * 2005-08-31 2007-11-08 인하대학교 산학협력단 섬모충류 특이적 프라이머 및 이를 이용한 섬모충류 검출방법

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US5958689A (en) * 1996-05-22 1999-09-28 Monterey Bay Aquarium Research Institute Detection of toxigenic marine diatoms of the genus Pseudo-nitzschia

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MONIZ, M. B. J. ET AL.: "Barcoding diatoms: Is there a good marker?", MOLECULAR ECOLOGY RESOURCES, vol. 9, no. SUPPL., 9 May 2009 (2009-05-09), pages 65 - 74 *
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Also Published As

Publication number Publication date
KR20110105669A (ko) 2011-09-27
WO2011115366A2 (ko) 2011-09-22
US20130123125A1 (en) 2013-05-16
KR101403351B1 (ko) 2014-06-09

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