AR065125A1 - Celulas hospederas geneticamente modificadas para niveles de actividad p450 incrementados y metodos de uso de las mismas - Google Patents
Celulas hospederas geneticamente modificadas para niveles de actividad p450 incrementados y metodos de uso de las mismasInfo
- Publication number
- AR065125A1 AR065125A1 ARP080100415A ARP080100415A AR065125A1 AR 065125 A1 AR065125 A1 AR 065125A1 AR P080100415 A ARP080100415 A AR P080100415A AR P080100415 A ARP080100415 A AR P080100415A AR 065125 A1 AR065125 A1 AR 065125A1
- Authority
- AR
- Argentina
- Prior art keywords
- genetically modified
- host cell
- modified host
- cytochrome
- enzyme
- Prior art date
Links
- 230000000694 effects Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 abstract 3
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 abstract 3
- 238000012258 culturing Methods 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 150000003505 terpenes Chemical class 0.000 abstract 2
- 101150053185 P450 gene Proteins 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 230000001590 oxidative effect Effects 0.000 abstract 1
- 230000036542 oxidative stress Effects 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000035882 stress Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01037—5-Aminolevulinate synthase (2.3.1.37)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
- C12Y603/02—Acid—amino-acid ligases (peptide synthases)(6.3.2)
- C12Y603/02002—Glutamate-cysteine ligase (6.3.2.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
- C12Y603/02—Acid—amino-acid ligases (peptide synthases)(6.3.2)
- C12Y603/02003—Glutathione synthase (6.3.2.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente solicitud se refiere a células hospederas genéticamente modificadas que exhiben niveles de actividad modificada de uno o más productos de gen, de manera que cuando una enzima P450 del citocromo es producida en células hospederas genéticamente modificadas, los niveles de actividad modificadas de uno o más productos de gen proporcionan produccion mejorada y/o actividad de la enzima P450 del citocromo. La presente solicitud proporciona métodos para producir una enzima P450 del citocromo en una célula hospedera, en general, que involucra cultivar una célula hospedera genéticamente modificada sujeto en un medio de cultivo adecuado. La presente solicitud además proporciona métodos para producir un producto de una oxidacion dependiente de P450, en general, que involucra cultivar una célula hospedera genéticamente modificada sujeto, en un medio de cultivo adecuado. Reivindicacion 1: Una célula hospedera genéticamente modificada, caracterizada porque dicha célula hospedera genéticamente modificada comprende un ácido nucleico que comprende una secuencia de nucleotido que codifica un producto de gen relacionado con el estrés oxidativo, en donde la produccion del producto de gen relacionado con el estrés oxidativo proporciona produccion incrementada de un isoprenoide o precursor isoprenoide por la célula hospedera genéticamente modificada, comparada con una célula hospedera de control no modificada genéticamente con el ácido nucleico.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US88749307P | 2007-01-31 | 2007-01-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR065125A1 true AR065125A1 (es) | 2009-05-20 |
Family
ID=39674698
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP080100415A AR065125A1 (es) | 2007-01-31 | 2008-02-01 | Celulas hospederas geneticamente modificadas para niveles de actividad p450 incrementados y metodos de uso de las mismas |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20080233623A1 (es) |
| AR (1) | AR065125A1 (es) |
| TW (1) | TW200846470A (es) |
| WO (1) | WO2008094546A2 (es) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
| US9637746B2 (en) | 2008-12-15 | 2017-05-02 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways |
| ES2718844T3 (es) * | 2010-05-07 | 2019-07-04 | Greenlight Biosciences Inc | Métodos para controlar el flujo en rutas metabólicas mediante la reubicación de enzimas |
| US8916358B2 (en) | 2010-08-31 | 2014-12-23 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through protease manipulation |
| CA2846893A1 (en) | 2011-09-09 | 2013-03-14 | Greenlight Biosciences, Inc. | Cell-free preparation of carbapenems |
| WO2013052669A1 (en) * | 2011-10-06 | 2013-04-11 | University Of Wyoming | Genetically inducible hydrogen peroxide resistance and production |
| US20150328337A1 (en) * | 2012-12-19 | 2015-11-19 | The Johns Hopkins University | Protection from oxidative damage by gene transfer by glutamate cysteine ligase and glutathione synthase |
| JP6483687B2 (ja) | 2013-08-05 | 2019-03-13 | グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． | プロテアーゼ切断部位を有する操作されたタンパク質 |
| BR112017020690A2 (pt) | 2015-03-30 | 2018-06-26 | Greenlight Biosciences Inc | produção livre de células de ácido ribonucleico |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| WO2017034942A1 (en) * | 2015-08-21 | 2017-03-02 | Manus Biosynthesis, Inc. | Increasing productivity of e. coli host cells that functionally express p450 enzymes |
| CN109196109B (zh) | 2016-04-06 | 2024-06-25 | 绿光生物科技股份有限公司 | 无细胞生成核糖核酸 |
| WO2017214159A1 (en) * | 2016-06-06 | 2017-12-14 | The Regents Of The University Of California | A host cell modified to produce lactams |
| US11203761B2 (en) | 2016-08-26 | 2021-12-21 | Codex Dna, Inc. | Genetically engineered Vibrio sp. and uses thereof |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| AU2018205503A1 (en) | 2017-01-06 | 2019-07-25 | Greenlight Biosciences, Inc. | Cell-free production of sugars |
| EP3425051A1 (en) * | 2017-07-05 | 2019-01-09 | DECHEMA -Forschungsinstitut | Method for the production of isoprenoids by heterologous expression of a protein in a microorganism |
| BR112020007068A2 (pt) | 2017-10-11 | 2020-10-06 | Greenlight Biosciences, Inc. | métodos e composições para produção de nucleosídeo trifosfato e ácido ribonucleico |
| CN111699247A (zh) | 2017-12-07 | 2020-09-22 | 齐默尔根公司 | 通过发酵产生(6e)-8-羟基香叶醇的经改造的生物合成途径 |
| WO2019126778A1 (en) | 2017-12-21 | 2019-06-27 | Zymergen Inc. | Nepetalactol oxidoreductases, nepetalactol synthases, and microbes capable of producing nepetalactone |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1063297B1 (en) * | 1999-06-22 | 2008-12-17 | Korea Kumho Petrochemical Co. Ltd. | Farnesyl pyrophosphate synthase (FPS) derived from seedlings of sunflower (Helianthus annus) |
| US7192751B2 (en) * | 2001-12-06 | 2007-03-20 | The Regents Of The University Of California | Biosynthesis of amorpha-4,11-diene |
| US7172886B2 (en) * | 2001-12-06 | 2007-02-06 | The Regents Of The University Of California | Biosynthesis of isopentenyl pyrophosphate |
| CN101023181A (zh) * | 2004-05-21 | 2007-08-22 | 加利福尼亚大学董事会 | 提高类异戊二烯化合物的产生的方法 |
-
2008
- 2008-01-28 WO PCT/US2008/001158 patent/WO2008094546A2/en not_active Ceased
- 2008-01-29 US US12/021,974 patent/US20080233623A1/en not_active Abandoned
- 2008-01-30 TW TW097103506A patent/TW200846470A/zh unknown
- 2008-02-01 AR ARP080100415A patent/AR065125A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20080233623A1 (en) | 2008-09-25 |
| WO2008094546A3 (en) | 2009-02-12 |
| TW200846470A (en) | 2008-12-01 |
| WO2008094546A2 (en) | 2008-08-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |