[go: up one dir, main page]

NO20072845L - Seksjon av vertsceller som uttrykker hoye nivaer av proteiner - Google Patents

Seksjon av vertsceller som uttrykker hoye nivaer av proteiner

Info

Publication number
NO20072845L
NO20072845L NO20072845A NO20072845A NO20072845L NO 20072845 L NO20072845 L NO 20072845L NO 20072845 A NO20072845 A NO 20072845A NO 20072845 A NO20072845 A NO 20072845A NO 20072845 L NO20072845 L NO 20072845L
Authority
NO
Norway
Prior art keywords
polypeptide
interest
selectable marker
host cells
cells expressing
Prior art date
Application number
NO20072845A
Other languages
English (en)
Other versions
NO344469B1 (no
Inventor
Arie Pieter Otte
Theodorus Hendrikus Jaco Kwaks
Richard George Antonius Sewalt
Henricus Johannes Mar Blokland
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Publication of NO20072845L publication Critical patent/NO20072845L/no
Publication of NO344469B1 publication Critical patent/NO344469B1/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Oppfinnelsen skaffer til veie et DNA-molekyl som består av en multisistronisk transkripsjonsenhet som koder for i) et selektabelt markørpolypeptid som virker funksjonelt i en eukaryot vertscelle, og for ii) et polypeptid av interesse, der polypeptidet av interesse besitter en translasjons-initierings-sekvens separat fra den tilhørende det selektable markørpolypeptidet, karakterisert ved at den kodende sekvensne for polypeptidet av interesse befinner seg nedstrøms for den kodende sekvensen til den selektable markøren i nevnte multisistroniske transkripsjonsenhet, og der nukleinsyre-sekvensen som koder for det selektable markørpolypeptidet består av en mutasjon som senker translasjons-effektiviteten til den selektable markøren i en eukaryot vertscelle. Oppfinnelsen skaffer også til veie metoder for å oppnå vertsceller som uttrykker et polypeptid av interesse, der nevnte vertsceller består av DNA-molekylene, i henhold til den foreliggende oppfinnelsen. Oppfinnelsen sørger videre for produksjon av polypeptidet av interesse, og innebærer dyrking av vertsceller som består av DNA-molekylene, i henhold til den foreliggende oppfinnelsen. ?? ?? ?? ??
NO20072845A 2004-11-08 2007-06-05 Seksjon av vertsceller som uttrykker høye nivåer av proteiner NO344469B1 (no)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US62630104P 2004-11-08 2004-11-08
EP04105593 2004-11-08
US69661005P 2005-07-05 2005-07-05
PCT/EP2005/055794 WO2006048459A2 (en) 2004-11-08 2005-11-07 Selection of host cells expressing protein at high levels

Publications (2)

Publication Number Publication Date
NO20072845L true NO20072845L (no) 2007-06-05
NO344469B1 NO344469B1 (no) 2019-12-30

Family

ID=36090897

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20072845A NO344469B1 (no) 2004-11-08 2007-06-05 Seksjon av vertsceller som uttrykker høye nivåer av proteiner

Country Status (13)

Country Link
US (1) US20060141577A1 (no)
EP (1) EP1809750B1 (no)
JP (1) JP5291341B2 (no)
KR (1) KR101271884B1 (no)
AU (1) AU2005300503B2 (no)
BR (1) BRPI0517380A (no)
CA (1) CA2581422C (no)
EA (1) EA010863B1 (no)
IL (1) IL183035A0 (no)
MX (1) MX2007004595A (no)
NO (1) NO344469B1 (no)
PL (1) PL1809750T3 (no)
WO (1) WO2006048459A2 (no)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654442A (en) * 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
EP1573058B1 (en) * 2002-12-18 2011-09-14 ChromaGenics B.V. A method for improving protein production
EP1572994B1 (en) 2002-12-20 2007-02-21 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
CN101090968B (zh) 2004-11-08 2011-10-26 科罗迈吉尼科斯公司 高水平表达蛋白质的宿主细胞的选择
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20070065912A1 (en) 2005-07-21 2007-03-22 Abbott Laboratories Multiple Gene Expression including sORF Constructs and Methods with Polyproteins, Pro-Proteins, and Proteolysis
CN101389763B (zh) * 2006-02-21 2013-01-23 科罗迈吉尼科斯公司 高水平表达蛋白质的宿主细胞的选择
WO2007107578A1 (en) 2006-03-20 2007-09-27 Chromagenics B.V. Expression augmenting dna fragments, use thereof, and methods for finding thereof
PL2064337T3 (pl) * 2006-09-20 2012-12-31 Genzyme Corp Oparty na FACS i białku reporterowym układ do wysokowydajnego opracowywania białek terapeutycznych
US20080274137A1 (en) * 2007-05-02 2008-11-06 Jean Christophe Francis Audonnet DNA plasmids having improved expression and stability
CN102016025B (zh) 2008-02-27 2015-09-16 国立大学法人北海道大学 用于用动物细胞大量生产源自外源基因的蛋白质的表达载体及其应用
CN102177235A (zh) * 2008-09-08 2011-09-07 赛莱克蒂斯公司 切割来自谷氨酰胺合成酶基因的dna靶序列的大范围核酸酶变体及其用途
EP2385115B1 (en) * 2008-12-22 2015-09-09 National University Corporation Hokkaido University Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof
DK2443239T3 (en) 2009-06-15 2016-02-15 Cellagenics B V New stringent selection markers
WO2011113841A1 (en) * 2010-03-16 2011-09-22 Robert Steinfeld Eukaryotic vector
EP2582816B1 (en) 2010-06-15 2017-12-20 Cellagenics B.V. Novel intergenic elements for enhancing gene expression
EP2611915B1 (en) 2010-09-01 2015-04-15 Cellagenics B.V. Nucleic acid fragments from a ribosomal protein promoter for enhancing gene expression
JP6297559B2 (ja) * 2012-07-31 2018-03-20 バジル・エム・ハンタッシュＢａｓｉｌ Ｍ． ＨＡＮＴＡＳＨ Ｈｌａｇ改変された細胞および方法
WO2016071306A1 (en) 2014-11-04 2016-05-12 Crucell Holland B.V. Therapeutic hpv16 vaccines
MY195389A (en) 2015-08-20 2023-01-18 Janssen Vaccines & Prevention Bv Therapeutic HPV18 Vaccines
IL258538B2 (en) 2015-10-09 2023-04-01 Genzyme Corp Early isolation after the infection of cells (epic) for the production of biological material
US10517944B2 (en) 2016-05-02 2019-12-31 Janssen Vaccines & Prevention B.V. Therapeutic HPV vaccine combinations
PL3523440T3 (pl) 2016-10-07 2025-06-09 Genzyme Corporation Wczesna izolacja komórek po transfekcji (epic) do wytwarzania środków biologicznych
EP3805396B1 (en) * 2018-05-24 2024-09-11 National University Corporation Hokkaido University Novel vector and use thereof
WO2020036181A1 (ja) * 2018-08-13 2020-02-20 Ｓｐｉｂｅｒ株式会社 細胞を単離又は同定する方法及び細胞集団
EP3856910B1 (en) * 2018-09-24 2024-11-20 Merck Sharp & Dohme LLC Expression vectors for eukaryotic expression systems
IL302786A (en) 2020-11-19 2023-07-01 Genzyme Corp Flow cytometry attenuated reporter expression (flare) multiple reporter system and methods of use thereof

Family Cites Families (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3700633A (en) * 1971-05-05 1972-10-24 Shell Oil Co Selectively hydrogenated block copolymers
US3821148A (en) * 1973-09-28 1974-06-28 Exxon Research Engineering Co Plasticized thermoplastic block copolymers
US4122134A (en) * 1974-02-13 1978-10-24 Sumitomo Chemical Company, Limited Method for producing transparent block copolymer resin
US3965830A (en) * 1974-12-05 1976-06-29 Usm Corporation Assembly for automatic bar tacking
US4267284A (en) * 1977-01-07 1981-05-12 Phillips Petroleum Company Tough, transparent articles from styrene polymers blended with certain block-random copolymers
US4965196A (en) 1983-01-19 1990-10-23 Genentech, Inc. Polycistronic expression vector construction
FR2569723B1 (fr) * 1984-08-31 1986-09-19 Centre Nat Rech Scient Application des antibiotiques de la famille des phleomycines a titre d'agent de selection dans le domaine du genie genetique
US4835200A (en) * 1986-12-19 1989-05-30 Shell Oil Company Color stable hot melt adhesive
US4882384A (en) * 1988-02-01 1989-11-21 Shell Oil Company Modified block copolymers
CA2063799A1 (en) * 1989-07-24 1991-01-25 Frank S. Genbauffe Prevention of internal initiation
US5256568A (en) * 1990-02-12 1993-10-26 Regeneron Phamaceuticals, Inc. Vectors and transformed most cells for recombinant protein production with reduced expression of selectable markers
JP3438889B2 (ja) 1991-03-29 2003-08-18 ジェネンテク，インコーポレイテッド 所望のタンパク質を高レベルに発現させる組換え宿主細胞の選別法
US6319707B1 (en) * 1991-08-12 2001-11-20 Fred Hutchinson Cancer Research Center Board Regents Of The University Of Washington Cap-independent multicistronic retroviral vectors
JP2686025B2 (ja) * 1992-08-25 1997-12-08 理化学研究所 ブラストサイジンｓデアミナーゼ遺伝子
US5648267A (en) 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
DE669986T1 (de) 1992-11-13 1996-10-10 Idec Pharma Corp Völlig unfunktionelle konsensus-kozak-sequenzen zur säugetier-exprimierung.
US5610053A (en) * 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
WO1994023046A1 (en) 1993-04-07 1994-10-13 The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services Dna sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5561053A (en) 1994-08-05 1996-10-01 Genentech, Inc. Method for selecting high-expressing host cells
US5627033A (en) 1995-03-28 1997-05-06 Research & Diagnostics Systems, Inc. Mammalian expression vectors
EP1445322B2 (en) 1995-06-15 2012-06-06 Crucell Holland B.V. Packaging systems for human recombinant adenovirus to be used in gene therapy
DE19523585A1 (de) * 1995-06-29 1997-01-02 Basf Ag Schlagzähe Formmasse aus Styrolpolymeren
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
DE19621688A1 (de) * 1996-05-30 1997-12-04 Basf Ag Transparente, nicht-blockende Folie
US6107477A (en) * 1996-09-26 2000-08-22 Aurora Biosciences Corporation Non-optimal Kozaks sequences
CA2283253A1 (en) 1997-03-04 1998-09-11 Baxter International Inc. Adenovirus e1-complementing cell lines
US5888809A (en) * 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
DE19832245A1 (de) 1998-07-17 2000-01-20 Heidelberger Druckmasch Ag Verfahren zum Bedienen von Geräten in der Reproduktionstechnik
WO2000005393A2 (en) 1998-07-21 2000-02-03 Cobra Therapeutics Limited A polynucleotide comprising a ubiquitous chromatin opening element (ucoe)
US6689606B2 (en) 1998-07-21 2004-02-10 M.L. Laboratories Plc Polynucleotide
US6872524B1 (en) * 1998-08-14 2005-03-29 Chromagenics B.V. Method of detecting an expression-enhancing sequence
US6800457B2 (en) * 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US6521419B1 (en) * 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
WO2000023606A1 (en) * 1998-10-22 2000-04-27 Medical College Of Georgia Institute, Inc. Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors
ATE289354T1 (de) 1999-04-15 2005-03-15 Crucell Holland Bv Verwendung von adenovirus e1 protein sequenzen kodierenden, zur herstellung von rekombinanten proteinen in menschlichen zellen.
US6391981B1 (en) * 1999-04-23 2002-05-21 Kraton Polymers Us Llc Increased throughput in the manufacture of anionic polymers by reduction in polymer cement viscosity through the addition of metal alkyls
JP2003504016A (ja) 1999-06-30 2003-02-04 アメリカ合衆国 遺伝子発現の制御のための特異的エンハンサー遮断活性を有する絶縁体としてのｄｎａ結合タンパク質および配列
NZ529329A (en) * 1999-08-25 2005-06-24 Immunex Corp Compositions and methods for improved cell culture
US6316253B1 (en) * 1999-11-01 2001-11-13 Chiron Corporation Expression vectors, transfection systems, and method of use thereof
US6558948B1 (en) 1999-11-23 2003-05-06 Stefan Kochanek Permanent amniocytic cell line, its production and use for the production of gene transfer vectors
AU2001238178A1 (en) * 2000-02-11 2001-08-20 Millennium Pharmaceuticals, Inc. Seven-transmembrane proteins/g-protein coupled receptors
US6586205B1 (en) * 2000-02-11 2003-07-01 Millennium Pharmaceuticals, Inc. 43239 a novel GPCR-like molecule and uses thereof
KR100996016B1 (ko) 2000-09-20 2010-11-22 밀리포어 코포레이션 폴리뉴클레오티드
ATE437233T1 (de) 2001-01-26 2009-08-15 Selexis Sa Matrix-anheftungsregionen und verfahren zu deren verwendung
US20020155540A1 (en) * 2001-02-14 2002-10-24 Malla Padidam Method to reduce transcriptional interference between tandem genes
FR2821855B1 (fr) * 2001-03-09 2004-04-02 Cayla Genes synthetiques et plasmides bacteriens depourvus de cpg
TWI324181B (en) * 2001-04-16 2010-05-01 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
CN1533432A (zh) 2001-06-04 2004-09-29 MLʵ���ҹ������޹�˾ 高水平、大规模生产重组蛋白的组合体和方法
CA2812799C (en) * 2001-07-04 2016-01-26 Chromagenics B.V. Dna sequences comprising gene transcription regulatory qualities and methods for detecting and using such dna sequences
US20040038304A1 (en) * 2002-03-28 2004-02-26 Gala Design, Inc. Antibody libraries
EP1513936A2 (en) * 2002-06-14 2005-03-16 Chromagenics B.V. Use of repression blocking sequences in methods for enhancing gene expression
WO2003106684A2 (en) * 2002-06-14 2003-12-24 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
AU2003263161A1 (en) 2002-09-20 2004-04-08 Novo Nordisk A/S Method for making recombinant proteins
EP1573058B1 (en) * 2002-12-18 2011-09-14 ChromaGenics B.V. A method for improving protein production
EP1572994B1 (en) * 2002-12-20 2007-02-21 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
IN2014DN02484A (no) 2003-10-24 2015-05-15 Selexis Sa
US7825232B2 (en) * 2004-07-08 2010-11-02 Chromagenics B.V. Sequence for improving expression of nucleic acid
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) * 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
CN101090968B (zh) * 2004-11-08 2011-10-26 科罗迈吉尼科斯公司 高水平表达蛋白质的宿主细胞的选择
WO2007107578A1 (en) * 2006-03-20 2007-09-27 Chromagenics B.V. Expression augmenting dna fragments, use thereof, and methods for finding thereof

Also Published As

Publication number Publication date
AU2005300503A1 (en) 2006-05-11
BRPI0517380A (pt) 2008-10-07
JP2008518613A (ja) 2008-06-05
EP1809750A2 (en) 2007-07-25
KR101271884B1 (ko) 2013-06-05
EA010863B1 (ru) 2008-12-30
CA2581422C (en) 2012-05-22
MX2007004595A (es) 2007-08-15
EA200701023A1 (ru) 2007-10-26
NO344469B1 (no) 2019-12-30
WO2006048459A3 (en) 2006-08-10
CA2581422A1 (en) 2006-05-11
WO2006048459A2 (en) 2006-05-11
KR20070090151A (ko) 2007-09-05
PL1809750T3 (pl) 2012-08-31
IL183035A0 (en) 2007-09-20
AU2005300503B2 (en) 2010-12-16
JP5291341B2 (ja) 2013-09-18
HK1101264A1 (en) 2007-10-12
EP1809750B1 (en) 2012-03-21
US20060141577A1 (en) 2006-06-29

Similar Documents

Publication Publication Date Title
NO20072845L (no) Seksjon av vertsceller som uttrykker hoye nivaer av proteiner
ZA200702046B (en) Selection of host cells expressing protein at high levels
DK2013349T3 (da) Selektion af værtsceller, der udtrykker protein ved høje niveauer
EA201691990A1 (ru) Получение библиотек вариантов белков, экспрессированных в эукариотических клетках, и применение их для отбора связывающих молекул
WO2008005847A3 (en) Method of producing factor viii proteins by recombinant methods
EA200901110A1 (ru) Рекомбинантная клетка-хозяин для получения соединения, представляющего интерес
WO2004056981A3 (en) Polypeptides having cellobiohydrolase ii activity and polynucleotides encoding same
WO2009137145A3 (en) Transcriptional control in alicyclobacillus acidocaldarius and associated genes, proteins, and methods
EA201170752A1 (ru) Растения с повышенной устойчивостью к абиотическому стрессу и/или улучшенными характеристиками урожайности и способ их получения
WO2011021105A3 (en) Generation and reproduction of dna sequences and analysis of polymorphisms and mutations by using error-correcting codes
DK1994149T3 (da) Nye fluorescerende proteiner og fremgangsmåder til anvendelse deraf
ATE546464T1 (de) Verfahren zur produktion von rekombinantem protein in cho-zellen
BR0215665B1 (pt) Proteína da endotoxina delta quimérica cry1ea e cry1ca
EP2261362A3 (en) Methods for improving crop plant architecture and yield
ATE509109T1 (de) Expressionssystem zur antibiotikafreien produktion von polypeptiden
EP2325197A3 (en) Expression control elements from the Lemnaceae family
BR0318314A (pt) molécula de ácido nucléico isolada que codifica o fator de transcrição hahb-4, vetor, plantas transgênicas, semente, células hospedeiras, método para a produção de uma planta transgênica tolerante ao stress de água, molécula isolada de ácido nucléico, construto de ácido nucléico e método para expressar pelo menos uma proteìna de interesse em uma célula hospedeira
DK1572133T3 (da) Fremgangsmåde til identificering af modulatorer af den humane orexin-2-receptor
RU2014129220A (ru) Новый аттенуированный полиовирус: pv-1 mono-cre-x
DE602004026474D1 (de) Rekombinanter faktor viii mit erhöhter spezifischer aktivität
CN101281201A (zh) 利用内含肽反式剪接制作蛋白质分子量标准的方法
ATE445009T1 (de) Signalpeptid zur herstellung eines polypeptids
DE602006020045D1 (de) Membrantranslozierende peptide
WO2020185669A3 (en) Fusion polypeptides
EA200702009A1 (ru) Усовершенствованный способ экспрессирующего клонирования в клетке-хозяине

Legal Events

Date Code Title Description
MM1K Lapsed by not paying the annual fees