MX9706841A - Variantes de proteinasa k que tienen adsorcion reducida e hidrolisis incrementada. - Google Patents
Variantes de proteinasa k que tienen adsorcion reducida e hidrolisis incrementada.Info
- Publication number
- MX9706841A MX9706841A MX9706841A MX9706841A MX9706841A MX 9706841 A MX9706841 A MX 9706841A MX 9706841 A MX9706841 A MX 9706841A MX 9706841 A MX9706841 A MX 9706841A MX 9706841 A MX9706841 A MX 9706841A
- Authority
- MX
- Mexico
- Prior art keywords
- proteinase
- variants
- loop region
- wild
- acid sequence
- Prior art date
Links
- 230000003247 decreasing effect Effects 0.000 title abstract 2
- 230000007062 hydrolysis Effects 0.000 title abstract 2
- 238000006460 hydrolysis reaction Methods 0.000 title abstract 2
- 238000001179 sorption measurement Methods 0.000 title abstract 2
- 102000035195 Peptidases Human genes 0.000 title 1
- 108091005804 Peptidases Proteins 0.000 title 1
- 235000019833 protease Nutrition 0.000 title 1
- 108010067770 Endopeptidase K Proteins 0.000 abstract 7
- 125000003275 alpha amino acid group Chemical class 0.000 abstract 4
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
- C11D17/0047—Detergents in the form of bars or tablets
- C11D17/0065—Solid detergents containing builders
- C11D17/0073—Tablets
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
- C11D17/0047—Detergents in the form of bars or tablets
- C11D17/0065—Solid detergents containing builders
- C11D17/0069—Laundry bars
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
- C11D17/0047—Detergents in the form of bars or tablets
- C11D17/0065—Solid detergents containing builders
- C11D17/0073—Tablets
- C11D17/0078—Multilayered tablets
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0052—Gas evolving or heat producing compositions
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0078—Compositions for cleaning contact lenses, spectacles or lenses
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Public Health (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invencion se refiere a variantes de proteinasa K que tienen una secuencia de amioácido modificada de secuencia de aminoácidos de proteinasa K de tipo silvestre, la secuencia de aminoácidos de tipo silvestre comprendiendo una primera region de lazo, una segunda region de lazo, una tercera region de lazo, una cuarta region de lazo; en la que la secuencia de aminoácidos comprende diferentes aminoácidos a los que ocurren en la proteinasa K de tipo silvestre (es decir, sustitucion) en posiciones identificadas específicas en una o más de las regiones de lazo, por medio de las cuales la variante de proteinasa K tiene adsorcion reducida con la proteinasa K de tipo silvestre; la presente invencion se refiere también a secuencias de ADN que codifican tales variantes de proteinasa K para una variante de superficies.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40157495A | 1995-03-09 | 1995-03-09 | |
| PCT/US1996/003005 WO1996028556A2 (en) | 1995-03-09 | 1996-03-06 | Proteinase k variants having decreased adsorption and increased hydrolysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX9706841A true MX9706841A (es) | 1997-11-29 |
Family
ID=23588288
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX9706841A MX9706841A (es) | 1995-03-09 | 1996-03-06 | Variantes de proteinasa k que tienen adsorcion reducida e hidrolisis incrementada. |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US6451574B1 (es) |
| EP (1) | EP0813601A2 (es) |
| JP (1) | JPH11501814A (es) |
| CN (1) | CN1183809A (es) |
| AU (1) | AU5092796A (es) |
| BR (1) | BR9607677A (es) |
| CA (1) | CA2214395C (es) |
| CZ (1) | CZ279997A3 (es) |
| HU (1) | HUP9801638A3 (es) |
| IL (1) | IL117350A0 (es) |
| MA (1) | MA23820A1 (es) |
| MX (1) | MX9706841A (es) |
| WO (1) | WO1996028556A2 (es) |
| ZA (1) | ZA961820B (es) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10204500A (ja) * | 1997-01-20 | 1998-08-04 | Soft Kyukyu Corp:Kk | 発泡錠剤洗浄剤 |
| AR015977A1 (es) * | 1997-10-23 | 2001-05-30 | Genencor Int | Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes |
| ES2367505T3 (es) | 1997-10-23 | 2011-11-04 | Danisco Us Inc. | Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes. |
| JP2000510524A (ja) * | 1997-12-22 | 2000-08-15 | ザ、プロクター、エンド、ギャンブル、カンパニー | ネオプルラナーゼを含有するクリーニング組成物 |
| AU2001254620A1 (en) * | 2000-04-28 | 2001-11-12 | Novozymes A/S | Lipolytic enzyme variant |
| US6803222B2 (en) | 2000-11-22 | 2004-10-12 | Kao Corporation | Alkaline proteases |
| DE10105911A1 (de) | 2001-02-09 | 2002-08-14 | Roche Diagnostics Gmbh | Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe |
| DE10105912A1 (de) * | 2001-02-09 | 2002-08-14 | Roche Diagnostics Gmbh | Rekombinante Proteinase K |
| DK200101090A (da) | 2001-07-12 | 2001-08-16 | Novozymes As | Subtilase variants |
| DE10153792A1 (de) | 2001-10-31 | 2003-05-22 | Henkel Kgaa | Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten |
| DE10162727A1 (de) | 2001-12-20 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| DE10162728A1 (de) | 2001-12-20 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| DE10163884A1 (de) | 2001-12-22 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| US7888093B2 (en) | 2002-11-06 | 2011-02-15 | Novozymes A/S | Subtilase variants |
| DE10260903A1 (de) * | 2002-12-20 | 2004-07-08 | Henkel Kgaa | Neue Perhydrolasen |
| US7294499B2 (en) | 2003-01-30 | 2007-11-13 | Novozymes A/S | Subtilases |
| US7153666B2 (en) | 2003-07-17 | 2006-12-26 | General Atomics | Methods and compositions for determination of glycated proteins |
| US7943385B2 (en) | 2006-07-25 | 2011-05-17 | General Atomics | Methods for assaying percentage of glycated hemoglobin |
| ES2404059T3 (es) | 2006-07-25 | 2013-05-23 | General Atomics | Procedimientos para analizar el porcentaje de hemoglobina glicada |
| US8673646B2 (en) * | 2008-05-13 | 2014-03-18 | General Atomics | Electrochemical biosensor for direct determination of percentage of glycated hemoglobin |
| CN105193640B (zh) * | 2014-06-24 | 2018-10-12 | 金普诺安蛋白质工程技术(北京)有限公司 | 蛋白酶k在皮肤保健和化妆品领域中的应用 |
| CN108603187A (zh) * | 2015-11-24 | 2018-09-28 | 诺维信公司 | 具有蛋白酶活性的多肽以及编码它们的多核苷酸 |
| CN109207460B (zh) * | 2018-10-23 | 2021-06-15 | 大连博格林生物科技有限公司 | 重组布氏白僵菌蛋白酶k突变体pk-m2及制备方法 |
| CN109280656B (zh) * | 2018-10-23 | 2021-06-15 | 大连博格林生物科技有限公司 | 重组布氏白僵菌蛋白酶k突变体pk-m1及制备方法 |
| US10633644B1 (en) * | 2018-12-20 | 2020-04-28 | New England Biolabs, Inc. | Proteinases with improved properties |
| CN112592931A (zh) * | 2020-12-31 | 2021-04-02 | 安徽丰原发酵技术工程研究有限公司 | 一种生产重组蛋白酶k的方法 |
| CN113913412B (zh) * | 2021-10-13 | 2023-10-03 | 湖北大学 | 蛋白酶k突变体及其制备方法 |
| CN114196656B (zh) * | 2021-12-29 | 2023-10-24 | 南京巨匠生物科技有限公司 | 一种蛋白酶k突变体及其表达载体的构建和应用 |
| CN115775591B (zh) * | 2023-01-10 | 2023-06-09 | 华测检测认证集团股份有限公司 | 一种引物设计方法、装置、设备及可读存储介质 |
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| US4980288A (en) | 1986-02-12 | 1990-12-25 | Genex Corporation | Subtilisin with increased thermal stability |
| US5240632A (en) * | 1986-03-26 | 1993-08-31 | Amway Corporation | Machine dishwasher water spot control composition |
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| NZ221627A (en) | 1986-09-09 | 1993-04-28 | Genencor Inc | Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios |
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| US5260207A (en) * | 1987-04-06 | 1993-11-09 | Enzon Labs Inc. | Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins |
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-
1996
- 1996-03-04 IL IL11735096A patent/IL117350A0/xx unknown
- 1996-03-06 CN CN96193792A patent/CN1183809A/zh active Pending
- 1996-03-06 HU HU9801638A patent/HUP9801638A3/hu unknown
- 1996-03-06 CA CA002214395A patent/CA2214395C/en not_active Expired - Fee Related
- 1996-03-06 CZ CZ972799A patent/CZ279997A3/cs unknown
- 1996-03-06 BR BR9607677A patent/BR9607677A/pt not_active Application Discontinuation
- 1996-03-06 JP JP8527690A patent/JPH11501814A/ja active Pending
- 1996-03-06 WO PCT/US1996/003005 patent/WO1996028556A2/en not_active Ceased
- 1996-03-06 AU AU50927/96A patent/AU5092796A/en not_active Abandoned
- 1996-03-06 EP EP96907182A patent/EP0813601A2/en not_active Withdrawn
- 1996-03-06 MX MX9706841A patent/MX9706841A/es unknown
- 1996-03-06 ZA ZA961820A patent/ZA961820B/xx unknown
- 1996-03-07 MA MA24177A patent/MA23820A1/fr unknown
-
1997
- 1997-09-23 US US08/935,887 patent/US6451574B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2214395A1 (en) | 1996-09-19 |
| US6451574B1 (en) | 2002-09-17 |
| ZA961820B (en) | 1996-09-10 |
| EP0813601A2 (en) | 1997-12-29 |
| AU5092796A (en) | 1996-10-02 |
| HUP9801638A3 (en) | 2001-01-29 |
| BR9607677A (pt) | 1998-07-07 |
| IL117350A0 (en) | 1996-07-23 |
| HUP9801638A2 (hu) | 1998-10-28 |
| CN1183809A (zh) | 1998-06-03 |
| CA2214395C (en) | 2001-10-30 |
| WO1996028556A3 (en) | 1997-07-17 |
| JPH11501814A (ja) | 1999-02-16 |
| WO1996028556A2 (en) | 1996-09-19 |
| CZ279997A3 (cs) | 1998-01-14 |
| MA23820A1 (fr) | 1996-10-01 |
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