MX2019015262A - Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso. - Google Patents
Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso.Info
- Publication number
- MX2019015262A MX2019015262A MX2019015262A MX2019015262A MX2019015262A MX 2019015262 A MX2019015262 A MX 2019015262A MX 2019015262 A MX2019015262 A MX 2019015262A MX 2019015262 A MX2019015262 A MX 2019015262A MX 2019015262 A MX2019015262 A MX 2019015262A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- making
- nucleic acids
- cell transcriptome
- throughput single
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Se proporciona en la presente métodos para preparar una biblioteca de secuenciación que incluye acidos nucleicos de una pluralidad de células individuales. En una modalidad, la biblioteca de secuenciación incluye ácidos nucleicos que representan los transcriptomas completos de la pluralidad de células individuales. En una modalidad, los ácidos nucleicos incluyen tres secuencias de índice. Tambien se proporcionan en la presente composiciones, tal como composiciones que incluyen los ácidos nucleicos que tienen tres secuencias de índice.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862680259P | 2018-06-04 | 2018-06-04 | |
| US201962821678P | 2019-03-21 | 2019-03-21 | |
| PCT/US2019/035422 WO2019236599A2 (en) | 2018-06-04 | 2019-06-04 | High-throughput single-cell transcriptome libraries and methods of making and of using |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2019015262A true MX2019015262A (es) | 2023-01-25 |
Family
ID=68769215
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2019015262A MX2019015262A (es) | 2018-06-04 | 2019-06-04 | Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso. |
| MX2023001088A MX2023001088A (es) | 2018-06-04 | 2019-12-16 | Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2023001088A MX2023001088A (es) | 2018-06-04 | 2019-12-16 | Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso. |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US20210102194A1 (es) |
| EP (2) | EP3810774B1 (es) |
| JP (3) | JP6986103B2 (es) |
| KR (2) | KR102507415B1 (es) |
| CN (1) | CN111247248A (es) |
| AU (3) | AU2019282158B2 (es) |
| BR (1) | BR112019027750A2 (es) |
| CA (1) | CA3072273C (es) |
| DK (1) | DK3810774T3 (es) |
| ES (1) | ES2966028T3 (es) |
| FI (1) | FI3810774T3 (es) |
| IL (2) | IL314667A (es) |
| MX (2) | MX2019015262A (es) |
| SG (1) | SG11202000905PA (es) |
| WO (1) | WO2019236599A2 (es) |
Families Citing this family (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10900065B2 (en) | 2014-11-14 | 2021-01-26 | University Of Washington | Methods and kits for labeling cellular molecules |
| FI3684949T3 (fi) | 2017-09-22 | 2024-07-17 | Univ Washington | Solumolekyylien kombinatorinen in situ -leimaaminen |
| US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
| US12163189B2 (en) | 2019-11-27 | 2024-12-10 | University Of Washington | Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria |
| WO2021168455A1 (en) * | 2020-02-20 | 2021-08-26 | The Regents Of The University Of California | Methods of spatially resolved single cell rna sequencing |
| EP4150106A4 (en) * | 2020-05-15 | 2024-07-10 | Integrated Dna Technologies, Inc. | LIGATURE-COUPLED PCR METHODS |
| CN111549025B (zh) * | 2020-06-08 | 2021-09-14 | 郑州大学第一附属医院 | 链置换引物和细胞转录组文库构建方法 |
| WO2021252617A1 (en) | 2020-06-09 | 2021-12-16 | Illumina, Inc. | Methods for increasing yield of sequencing libraries |
| US12359266B2 (en) * | 2020-06-18 | 2025-07-15 | Board Of Regents, The University Of Texas System | Tiled ClickSeq for targeted virus whole genome sequencing |
| CN117178187A (zh) * | 2020-07-22 | 2023-12-05 | 奧基生物技术有限公司 | 用于确定药物有效性的方法和系统 |
| EP4186060A4 (en) * | 2020-07-23 | 2024-01-03 | 10X Genomics, Inc. | SYSTEMS AND METHODS FOR DETECTING AND REMOVAL OF AGGREGATES FOR RETRIEVING CELL-ASSOCIATED BAR CODES |
| CN111996598A (zh) * | 2020-08-11 | 2020-11-27 | 杭州瀚因生命科技有限公司 | 一种单细胞染色质可及性的建库方法 |
| US20230265499A1 (en) * | 2020-08-14 | 2023-08-24 | Factorial Diagnostics, Inc. | In Situ Library Preparation for Sequencing |
| WO2022058339A1 (en) * | 2020-09-15 | 2022-03-24 | Dhapola Parashar | Method and system for subsampling of cells from single-cell genomics dataset |
| US12378596B2 (en) * | 2020-12-03 | 2025-08-05 | Roche Sequencing Solutions, Inc. | Whole transcriptome analysis in single cells |
| CN120041546A (zh) * | 2020-12-07 | 2025-05-27 | 中国科学院脑科学与智能技术卓越创新中心 | 一种单细胞转录组测序的方法 |
| CN112592968B (zh) * | 2020-12-27 | 2022-07-26 | 苏州科诺医学检验实验室有限公司 | 高通量测序用分子标签接头及其合成方法与应用 |
| WO2022143221A1 (zh) * | 2020-12-31 | 2022-07-07 | 中国科学院北京基因组研究所(国家生物信息中心) | 用于标记核酸分子的方法和试剂盒 |
| US12412100B2 (en) * | 2021-01-22 | 2025-09-09 | International Business Machines Corporation | Cell state transition features from single cell data |
| EP4298244A1 (en) | 2021-02-23 | 2024-01-03 | 10X Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
| EP4305205A4 (en) * | 2021-03-10 | 2025-08-06 | Factorial Diagnostics Inc | CELL BARCODE COMPOSITIONS AND RELATED METHODS |
| CN113160886B (zh) * | 2021-04-02 | 2023-04-07 | 山东大学 | 基于单细胞Hi-C数据的细胞类型预测系统 |
| CN115011679B (zh) * | 2021-05-12 | 2023-04-11 | 浙江大学 | 一种超高通量单细胞测序方法 |
| CN113652475A (zh) * | 2021-07-19 | 2021-11-16 | 中国辐射防护研究院 | 一种重金属铀诱导仓鼠卵巢细胞损伤的研究方法 |
| WO2023044475A1 (en) * | 2021-09-20 | 2023-03-23 | Faeth Therapeutics, Inc. | Methods of determining metabolic targets and designing nutrient modulating treatments |
| CN114107459B (zh) * | 2021-11-11 | 2022-12-06 | 浙江大学 | 一种基于寡核苷酸链杂交标记的高通量单细胞测序方法 |
| CA3227385A1 (en) * | 2021-12-23 | 2023-06-29 | Kazuo TORI | Methods and compositions for producing cell-source identifiable collections of nucleic acids |
| WO2023137292A1 (en) * | 2022-01-12 | 2023-07-20 | Jumpcode Genomics, Inc. | Methods and compositions for transcriptome analysis |
| US20250145986A1 (en) * | 2022-02-16 | 2025-05-08 | Factorial Diagnostics, Inc. | In Situ Library Preparation |
| JP2025512975A (ja) | 2022-04-07 | 2025-04-22 | イルミナ インコーポレイテッド | 改変シチジンデアミナーゼ及び使用方法 |
| US20250308625A1 (en) * | 2022-05-18 | 2025-10-02 | Yale University | Method for estimating a dynamic molecular program of a cell |
| CN114774527A (zh) * | 2022-05-20 | 2022-07-22 | 良渚实验室 | 一种高通量单细胞转录组测序方法及其应用 |
| EP4332234A1 (en) * | 2022-08-29 | 2024-03-06 | Ecole Polytechnique Federale De Lausanne (Epfl) | Improved microfluidic transcriptome based screening methods |
| WO2024073412A2 (en) * | 2022-09-26 | 2024-04-04 | The Rockefeller University | Compositions and methods for synthesizing multi-indexed sequencing libraries |
| EP4594343A1 (en) | 2022-09-30 | 2025-08-06 | Illumina, Inc. | Methods of using cpg binding proteins in mapping modified cytosine nucleotides |
| CN120153070A (zh) | 2022-09-30 | 2025-06-13 | 因美纳有限公司 | 解旋酶-胞苷脱氨酶复合物及其使用方法 |
| EP4594482A1 (en) | 2022-09-30 | 2025-08-06 | Illumina, Inc. | Cytidine deaminases and methods of use in mapping modified cytosine nucleotides |
| EP4627113A1 (en) | 2022-11-30 | 2025-10-08 | Illumina, Inc. | Chemoenzymatic correction of false positive uracil transformations |
| AU2023421715A1 (en) | 2023-01-06 | 2024-09-26 | Illumina, Inc. | Reducing uracils by polymerase |
| EP4662310A2 (en) * | 2023-02-07 | 2025-12-17 | Parse Biosciences, Inc. | Methods and kits for labeling cellular molecules for multiplex analysis |
| WO2024249466A1 (en) | 2023-05-31 | 2024-12-05 | Illumina, Inc. | False positive reduction by translesion polymerase repair |
| CN116497105B (zh) * | 2023-06-28 | 2023-09-29 | 浙江大学 | 基于末端转移酶的单细胞转录组测序试剂盒及测序方法 |
| WO2025072793A1 (en) | 2023-09-28 | 2025-04-03 | Illumina, Inc. | Altered cytidine deaminases and methods of use |
| WO2025081064A2 (en) | 2023-10-11 | 2025-04-17 | Illumina, Inc. | Thermophilic deaminase and methods for identifying modified cytosine |
| WO2025137222A1 (en) | 2023-12-19 | 2025-06-26 | Illumina, Inc. | Methylation detection assay |
| WO2025240731A1 (en) * | 2024-05-15 | 2025-11-20 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions to enrich target rnas for perturb-seq |
| CN120032727A (zh) * | 2025-01-26 | 2025-05-23 | 河北工业大学 | 基于锚点选择与图学习协同优化的单细胞多组学整合方法 |
| CN120319326B (zh) * | 2025-06-18 | 2025-10-10 | 杭州联川生物技术股份有限公司 | 基于元细胞的空间转录组细胞类型注释方法、设备和介质 |
Family Cites Families (88)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
| CA1341584C (en) | 1988-04-06 | 2008-11-18 | Bruce Wallace | Method of amplifying and detecting nucleic acid sequences |
| AU3539089A (en) | 1988-04-08 | 1989-11-03 | Salk Institute For Biological Studies, The | Ligase-based amplification method |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| EP0379559B1 (en) | 1988-06-24 | 1996-10-23 | Amgen Inc. | Method and reagents for detecting nucleic acid sequences |
| EP0425563B1 (en) | 1988-07-20 | 1996-05-15 | David Segev | Process for amplifying and detecting nucleic acid sequences |
| US5185243A (en) | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
| US5248607A (en) * | 1988-10-27 | 1993-09-28 | Ciba-Geigy Corporation | Monoclonal antibodies and hybridomas specific for green-oat phytochrome |
| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| US5573907A (en) | 1990-01-26 | 1996-11-12 | Abbott Laboratories | Detecting and amplifying target nucleic acids using exonucleolytic activity |
| KR950013953B1 (ko) | 1990-01-26 | 1995-11-18 | 애보트 래보라토리즈 | 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법 |
| US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| JPH06197762A (ja) * | 1992-12-28 | 1994-07-19 | Iseki & Co Ltd | タマネギのｄｎａ抽出法 |
| AU694187B2 (en) | 1994-02-07 | 1998-07-16 | Beckman Coulter, Inc. | Ligase/polymerase-mediated genetic bit analysis TM of single nucleotide polymorphisms and its use in genetic analysis |
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| CA2185239C (en) | 1994-03-16 | 2002-12-17 | Nanibhushan Dattagupta | Isothermal strand displacement nucleic acid amplification |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| JPH11513115A (ja) * | 1995-08-21 | 1999-11-09 | アメリカ合衆国 | Ｍｈｃクラスｉの発現の評価方法及びクラスｉの発現を調節することが可能なタンパク質 |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| ATE364718T1 (de) | 1997-04-01 | 2007-07-15 | Solexa Ltd | Verfahren zur vervielfältigung von nukleinsäure |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US7611869B2 (en) | 2000-02-07 | 2009-11-03 | Illumina, Inc. | Multiplexed methylation detection methods |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| AU8288101A (en) | 2000-07-07 | 2002-01-21 | Visigen Biotechnologies Inc | Real-time sequence determination |
| US7211414B2 (en) | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| AR031640A1 (es) | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
| US20030082584A1 (en) * | 2001-06-29 | 2003-05-01 | Liang Shi | Enzymatic ligation-based identification of transcript expression |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| ES2550513T3 (es) | 2002-08-23 | 2015-11-10 | Illumina Cambridge Limited | Nucleótidos modificados para secuenciación de polinucleótidos |
| CA2498764C (en) | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
| CA2528577C (en) | 2003-06-20 | 2012-08-07 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| EP2789383B1 (en) | 2004-01-07 | 2023-05-03 | Illumina Cambridge Limited | Molecular arrays |
| WO2006044078A2 (en) | 2004-09-17 | 2006-04-27 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
| WO2006064199A1 (en) | 2004-12-13 | 2006-06-22 | Solexa Limited | Improved method of nucleotide detection |
| EP1888743B1 (en) | 2005-05-10 | 2011-08-03 | Illumina Cambridge Limited | Improved polymerases |
| US20090264299A1 (en) | 2006-02-24 | 2009-10-22 | Complete Genomics, Inc. | High throughput genome sequencing on DNA arrays |
| CA2611671C (en) | 2005-06-15 | 2013-10-08 | Callida Genomics, Inc. | Single molecule arrays for genetic and chemical analysis |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| WO2007133831A2 (en) | 2006-02-24 | 2007-11-22 | Callida Genomics, Inc. | High throughput genome sequencing on dna arrays |
| GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| EP2021503A1 (en) | 2006-03-17 | 2009-02-11 | Solexa Ltd. | Isothermal methods for creating clonal single molecule arrays |
| EP4105644A3 (en) | 2006-03-31 | 2022-12-28 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
| WO2008051530A2 (en) | 2006-10-23 | 2008-05-02 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
| US7910302B2 (en) | 2006-10-27 | 2011-03-22 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| AU2007334393A1 (en) | 2006-12-14 | 2008-06-26 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| EP2121983A2 (en) | 2007-02-02 | 2009-11-25 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| WO2010003132A1 (en) | 2008-07-02 | 2010-01-07 | Illumina Cambridge Ltd. | Using populations of beads for the fabrication of arrays on surfaces |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| AU2011323107B2 (en) | 2010-11-05 | 2015-09-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
| EP2718465B1 (en) | 2011-06-09 | 2022-04-13 | Illumina, Inc. | Method of making an analyte array |
| WO2013044018A1 (en) | 2011-09-23 | 2013-03-28 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
| US8778849B2 (en) | 2011-10-28 | 2014-07-15 | Illumina, Inc. | Microarray fabrication system and method |
| EP2636427B1 (en) | 2012-01-16 | 2019-02-27 | Greatbatch Ltd. | Elevated hermetic feedthrough insulator adapted for side attachment of electrical conductors on the body fluid side of an active implantable medical device |
| EP2834622B1 (en) | 2012-04-03 | 2023-04-12 | Illumina, Inc. | Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing |
| US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
| US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
| KR102177225B1 (ko) | 2013-07-01 | 2020-11-10 | 일루미나, 인코포레이티드 | 촉매-무함유 표면 작용화 및 중합체 그라프팅 |
| US9677132B2 (en) | 2014-01-16 | 2017-06-13 | Illumina, Inc. | Polynucleotide modification on solid support |
| US10017759B2 (en) * | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
| CN104032377A (zh) * | 2014-06-30 | 2014-09-10 | 北京诺禾致源生物信息科技有限公司 | 单细胞转录组测序文库的构建方法及其应用 |
| LT3212684T (lt) | 2014-10-31 | 2020-04-10 | Illumina Cambridge Limited | Polimerai ir dnr kopolimero dangos |
| US10900065B2 (en) * | 2014-11-14 | 2021-01-26 | University Of Washington | Methods and kits for labeling cellular molecules |
| CN107427808B (zh) * | 2015-01-12 | 2020-10-23 | 10X基因组学有限公司 | 用于制备核酸测序文库的方法和系统以及用其制备的文库 |
| KR20200020997A (ko) * | 2015-02-10 | 2020-02-26 | 일루미나, 인코포레이티드 | 세포 성분을 분석하기 위한 방법 및 조성물 |
| US20180080061A1 (en) * | 2015-03-23 | 2018-03-22 | The Regents Of The University Of California | Biosynthetic labeling and separation of rna |
| EP3286326B1 (en) * | 2015-04-23 | 2025-01-22 | Becton, Dickinson and Company | Method for whole transcriptome amplification |
| US12060412B2 (en) * | 2016-03-21 | 2024-08-13 | The Broad Institute, Inc. | Methods for determining spatial and temporal gene expression dynamics in single cells |
| DK3488002T3 (da) * | 2016-07-22 | 2021-06-21 | Univ Oregon Health & Science | Enkeltcelle-helgenombiblioteker og kombinatoriske indekseringsfremgangsmåder til fremstilling deraf |
| KR102747205B1 (ko) * | 2016-12-29 | 2024-12-31 | 일루미나, 인코포레이티드 | 세포 구획 내의 생체분자에 대한 직교 접근 및 그의 태그부착을 위한 분석 시스템 |
-
2019
- 2019-06-04 CN CN201980003786.3A patent/CN111247248A/zh active Pending
- 2019-06-04 CA CA3072273A patent/CA3072273C/en active Active
- 2019-06-04 US US16/625,100 patent/US20210102194A1/en active Pending
- 2019-06-04 AU AU2019282158A patent/AU2019282158B2/en active Active
- 2019-06-04 FI FIEP19814056.8T patent/FI3810774T3/fi active
- 2019-06-04 ES ES19814056T patent/ES2966028T3/es active Active
- 2019-06-04 EP EP19814056.8A patent/EP3810774B1/en active Active
- 2019-06-04 DK DK19814056.8T patent/DK3810774T3/da active
- 2019-06-04 EP EP23187488.4A patent/EP4269618B1/en active Active
- 2019-06-04 MX MX2019015262A patent/MX2019015262A/es unknown
- 2019-06-04 KR KR1020227014338A patent/KR102507415B1/ko active Active
- 2019-06-04 IL IL314667A patent/IL314667A/en unknown
- 2019-06-04 KR KR1020207003217A patent/KR102393414B1/ko active Active
- 2019-06-04 BR BR112019027750A patent/BR112019027750A2/pt unknown
- 2019-06-04 JP JP2019570097A patent/JP6986103B2/ja active Active
- 2019-06-04 IL IL272234A patent/IL272234B2/en unknown
- 2019-06-04 SG SG11202000905PA patent/SG11202000905PA/en unknown
- 2019-06-04 WO PCT/US2019/035422 patent/WO2019236599A2/en not_active Ceased
- 2019-12-16 MX MX2023001088A patent/MX2023001088A/es unknown
-
2021
- 2021-11-04 AU AU2021261918A patent/AU2021261918B2/en active Active
- 2021-11-26 JP JP2021192377A patent/JP7581175B2/ja active Active
-
2024
- 2024-10-30 JP JP2024190580A patent/JP2025026864A/ja active Pending
-
2025
- 2025-03-13 AU AU2025201842A patent/AU2025201842A1/en active Pending
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX2019015262A (es) | Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso. | |
| MX2019015013A (es) | Secuenciación de células individuales de alto rendimiento con sesgo de amplificación reducido. | |
| CO2021006301A2 (es) | Composiciones y métodos de inmunoterapia | |
| SA518391294B1 (ar) | التحرير المتعدد للجينوم | |
| MX2019003674A (es) | Enzimas modificadoras de ácido nucleico guiadas por arn y métodos de uso de estas. | |
| ECSP14028704A (es) | Métodos y composiciones para la modificación de adn objetivo dirigida por arn y para la modulación de la transcripción dirigida por arn | |
| MX2018005392A (es) | Acidos nucleicos modificados dirigidos a acidos nucleicos. | |
| MX2017016688A (es) | Nuevas enzimas y sistemas crispr. | |
| MX2021004282A (es) | Construcciones de ácido nucleico y métodos de uso. | |
| MX2022002613A (es) | Metodos y composiciones para la integracion del genoma. | |
| CO2020005181A2 (es) | Polinucleótidos, composiciones y métodos para la edición del genoma | |
| WO2019090251A3 (en) | Nucleic acid indexing techniques | |
| CO2018000134A2 (es) | Factor ix modificado, y composiciones para la transferencia de genes a células, órganos y tejidos | |
| EA201992615A1 (ru) | Способы выделения клеток без использования трансгенных маркерных последовательностей | |
| EP3532075A4 (en) | VIRAL METHODS OF MANUFACTURING GENETICALLY MODIFIED CELLS | |
| AR102420A1 (es) | Sitios de integración en celulas cho y sus usos | |
| MX388784B (es) | Métodos y composiciones para la modificación genética dirigida a través de direccionamiento múltiple de una sola etapa. | |
| MX2018001617A (es) | Composiciones de crispr-cas9 diseñados y metodos de uso. | |
| MX2017011679A (es) | Polipeptidos de transposasa y sus usos. | |
| MX2016002266A (es) | Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso. | |
| AR104717A1 (es) | Plantas con una captación mejorada de sílice | |
| AR105760A1 (es) | Métodos y constructos para la modificación específica de ácidos nucleicos en plantas | |
| MX2021011847A (es) | Genotecas de celulas unicas de alto rendimiento y metodos para elaborarlas y usarlas. | |
| MX2017003420A (es) | Matrices polimericas de emision. | |
| MX2016015609A (es) | Animales con dipeptidil peptidasa iv (dpp4) humanizada. |