[go: up one dir, main page]

MX2018015360A - Un metodo eficiente y novedoso para reprogramar la sangre para las celulas madre pluripotentes inducidas. - Google Patents

Un metodo eficiente y novedoso para reprogramar la sangre para las celulas madre pluripotentes inducidas.

Info

Publication number
MX2018015360A
MX2018015360A MX2018015360A MX2018015360A MX2018015360A MX 2018015360 A MX2018015360 A MX 2018015360A MX 2018015360 A MX2018015360 A MX 2018015360A MX 2018015360 A MX2018015360 A MX 2018015360A MX 2018015360 A MX2018015360 A MX 2018015360A
Authority
MX
Mexico
Prior art keywords
reprogramming
stem cells
pluripotent stem
induced pluripotent
novel
Prior art date
Application number
MX2018015360A
Other languages
English (en)
Other versions
MX380817B (es
Inventor
Sareen Dhruv
A Ornelas Loren
Svendsen Clive
Original Assignee
Cedars Sinai Medical Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cedars Sinai Medical Center filed Critical Cedars Sinai Medical Center
Publication of MX2018015360A publication Critical patent/MX2018015360A/es
Publication of MX380817B publication Critical patent/MX380817B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/608—Lin28
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/02—Coculture with; Conditioned medium produced by embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Transplantation (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se describen en la presente métodos y composiciones relacionadas con la generación de células madre pluripotentes inducidas (iPSCs). Técnicas mejoradas para establecer una reprogramación altamente eficiente y reproducible utilizando vectores de plásmidos episómicos no integradores. Usando el protocolo de reprogramación descrito, uno puede reprogramar de manera consistente las células no-T con casi el 100% de éxito de fuentes de células no-T o no-B. Otras ventajas incluyen el uso de un medio de reprogramación definido E7 y el uso de un sustrato definido clínicamente compatible recombinante humano L-521. La generación de las iPSC a partir de estas fuentes de células sanguíneas permite la recapitulación de todo el repertorio genómico, la preservación de la fidelidad genómica y la estabilidad genómica mejorada.
MX2018015360A 2016-06-16 2017-06-16 Un método eficiente y novedoso para generar células madre pluripotentes inducidas a partir de células sanguíneas. MX380817B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15/184,241 US10221395B2 (en) 2016-06-16 2016-06-16 Efficient method for reprogramming blood to induced pluripotent stem cells
PCT/US2017/038041 WO2017219000A1 (en) 2016-06-16 2017-06-16 A novel and efficient method for reprogramming blood to induced pluripotent stem cells

Publications (2)

Publication Number Publication Date
MX2018015360A true MX2018015360A (es) 2019-08-21
MX380817B MX380817B (es) 2025-03-12

Family

ID=60659283

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018015360A MX380817B (es) 2016-06-16 2017-06-16 Un método eficiente y novedoso para generar células madre pluripotentes inducidas a partir de células sanguíneas.

Country Status (9)

Country Link
US (3) US10221395B2 (es)
EP (2) EP4265716A3 (es)
JP (3) JP7160690B2 (es)
KR (1) KR102388643B1 (es)
CN (2) CN109642212B (es)
AU (1) AU2017286794B2 (es)
CA (1) CA3025678A1 (es)
MX (1) MX380817B (es)
WO (1) WO2017219000A1 (es)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11572545B2 (en) 2016-06-16 2023-02-07 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US11970714B2 (en) 2016-06-16 2024-04-30 Cedars-Sinai Medical Center Method for reprogramming blood to induced pluripotent stem cells

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9719068B2 (en) 2010-05-06 2017-08-01 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
WO2015006725A2 (en) 2013-07-12 2015-01-15 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells from normal human mammary epithelial cells
EP3149156B1 (en) 2014-05-28 2021-02-17 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
WO2017123806A1 (en) 2016-01-12 2017-07-20 Cedars-Sinai Medical Center A method of non destructive monitoring of biological processes in microfluidic tissue culture systems
WO2017136462A2 (en) 2016-02-01 2017-08-10 EMULATE, Inc. Systems and methods for growth of intestinal cells in microfluidic devices
EP3534976A4 (en) 2016-11-04 2020-09-16 Children's Hospital Medical Center PATHOLOGICAL MODELS OF HEPATIC ORGANOIDS AND ASSOCIATED METHODS OF MANUFACTURE AND USE
EP3548507A4 (en) 2016-12-05 2020-07-15 Children's Hospital Medical Center COLON ORGANOIDS AND PROCESSES FOR THE PREPARATION AND USE THEREOF
US11913022B2 (en) 2017-01-25 2024-02-27 Cedars-Sinai Medical Center In vitro induction of mammary-like differentiation from human pluripotent stem cells
US11767513B2 (en) 2017-03-14 2023-09-26 Cedars-Sinai Medical Center Neuromuscular junction
US11414648B2 (en) 2017-03-24 2022-08-16 Cedars-Sinai Medical Center Methods and compositions for production of fallopian tube epithelium
EP3609997A4 (en) * 2017-04-14 2021-03-03 Children's Hospital Medical Center COMPOSITIONS OF STEM CELLS FROM MULTIPLE DONOR CELLS AND THEIR PREPARATION PROCEDURES
EP3694603A4 (en) 2017-10-10 2021-07-14 Children's Hospital Medical Center ESOPHAGUS TISSUE AND / OR ORGANOID COMPOSITIONS AND METHOD FOR MANUFACTURING THEREOF
US12379372B2 (en) 2017-12-21 2025-08-05 Children's Hospital Medical Center Digitalized human organoids and methods of using same
CA3092284A1 (en) 2018-03-16 2019-09-19 Cedars-Sinai Medical Center Methods and compositions for inducible expression of neurotrophic factors
US12161676B2 (en) 2018-03-23 2024-12-10 Cedars-Sinai Medical Center Methods of use of islet cells
EP3775161A4 (en) * 2018-04-06 2022-04-06 Cedars-Sinai Medical Center NEURODEGENERATIVE DISEASE MODELS DERIVED FROM HUMAN PLURIPOTENTIC STEM CELLS ON A MICROFLUIDIC CHIP
US11981918B2 (en) 2018-04-06 2024-05-14 Cedars-Sinai Medical Center Differentiation technique to generate dopaminergic neurons from induced pluripotent stem cells
US20210147811A1 (en) * 2018-04-09 2021-05-20 Cedars-Sinai Medical Center Methods for in vitro expansion of adult tissue stem cells
KR102887406B1 (ko) 2018-07-26 2025-11-19 칠드런즈 호스피탈 메디칼 센터 간-담도-췌장 조직 및 이를 제조하는 방법
WO2020056158A1 (en) 2018-09-12 2020-03-19 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
EP4048282A4 (en) * 2019-10-22 2024-01-10 Cedars-Sinai Medical Center NEURAL CORTICAL PROGENITOR CELLS FROM IPSC
WO2021207673A1 (en) * 2020-04-09 2021-10-14 The Regents of the University of Colorodo, a body corporate Compositions, methods and uses for production of hematopoietic stem cells (hscs)
CN116348592A (zh) * 2020-10-02 2023-06-27 菲特治疗公司 诱导性多能干细胞的改善的重编程、维持和保存
CN112322722B (zh) * 2020-11-13 2021-11-12 上海宝藤生物医药科技股份有限公司 检测16p11.2微缺失的引物探针组合物、试剂盒及其应用
KR20220163299A (ko) * 2021-06-02 2022-12-09 한국생명공학연구원 신규한 만능성 세포
CN113862221B (zh) * 2021-10-27 2024-04-30 上海爱萨尔生物科技有限公司 外周血单个核细胞的培养液及培养方法
CN116218786B (zh) * 2023-03-09 2024-01-23 山东大学齐鲁医院 一种多重基因编辑的通用型巨噬细胞及在制备抗肿瘤药物中的应用
CN120041395B (zh) * 2025-04-27 2025-08-01 南京鸿明生物科技有限公司 一种pbmc的重编程诱导多能干细胞制备方法及试剂盒

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US20090203141A1 (en) 2003-05-15 2009-08-13 Shi-Lung Lin Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant RNA agents
EP2137296A2 (en) 2007-03-23 2009-12-30 Wisconsin Alumni Research Foundation Somatic cell reprogramming
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
KR101532442B1 (ko) * 2007-12-10 2015-06-29 고쿠리츠 다이가쿠 호진 교토 다이가쿠 효율적인 핵 초기화 방법
ES2722198T3 (es) 2008-05-02 2019-08-08 Univ Kyoto Método de reprogramación nuclear
EP3447128A1 (en) 2008-06-04 2019-02-27 FUJIFILM Cellular Dynamics, Inc. Methods for the production of ips cells using non-viral approach
EP2315829B1 (en) 2008-08-08 2015-02-11 Mayo Foundation For Medical Education And Research Induced pluripotent stem cells
US20110231944A1 (en) * 2008-09-04 2011-09-22 Riken B cell-derived ips cells and application thereof
EP3450545B1 (en) 2008-10-24 2023-08-23 Wisconsin Alumni Research Foundation Pluripotent stem cells obtained by non-viral reprogramming
SG173469A1 (en) 2009-02-20 2011-09-29 Ventria Bioscience Cell culture media containing combinations of proteins
CN103087991B (zh) 2009-06-05 2018-06-12 富士胶片细胞动力公司 重编程t细胞和造血细胞的方法
WO2011016588A1 (en) 2009-08-07 2011-02-10 Kyoto University Method of efficiently establishing induced pluripotent stem cells
US20120301438A1 (en) * 2009-09-14 2012-11-29 The Johns Hopkins University Reprogramming Blood Cells to Pluripotent and Multipotent Stem Cells
ES2539487T3 (es) 2009-11-04 2015-07-01 Cellular Dynamics International, Inc. Reprogramación episómica con compuestos químicos
CN103025865A (zh) 2010-05-06 2013-04-03 干细胞医药有限公司 用于个性化医疗的干细胞库
US8048675B1 (en) 2010-05-12 2011-11-01 Ipierian, Inc. Integration-free human induced pluripotent stem cells from blood
US8691574B2 (en) 2010-06-15 2014-04-08 Cellular Dynamics International, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
US8765470B2 (en) 2010-08-04 2014-07-01 Cellular Dynamics International, Inc. Reprogramming immortalized B-cells to induced pluripotent stem cells
JP2013541951A (ja) 2010-10-01 2013-11-21 アメリカ合衆国 ｐ５３アイソフォームによる幹細胞機能の操作
JP2014520551A (ja) 2011-07-11 2014-08-25 セルラー ダイナミクス インターナショナル， インコーポレイテッド 細胞のリプログラミング方法およびゲノムの改変方法
WO2013040445A1 (en) 2011-09-15 2013-03-21 Whitehead Institute For Biomedical Research Arrays for cell-based screening and uses thereof
KR102048464B1 (ko) * 2011-09-22 2019-11-25 칼 트리그베이슨 라미닌 및 카데린를 포함하는 세포 배양 기질
WO2013062140A1 (en) 2011-10-28 2013-05-02 Kyoto University Method for efficiently inducing differentiation of pluripotent stem cells into hepatic lineage cells
EP2826855B1 (en) 2012-03-15 2018-08-29 iHeart Japan Corporation Myocardial sheet
WO2013177228A1 (en) 2012-05-22 2013-11-28 Loma Linda University Generation of integration/transgene-free stem cells
EP2853592B1 (en) 2012-05-23 2019-02-20 Kyoto University Highly efficient method for establishing artificial pluripotent stem cell
EP3456831B1 (en) 2013-04-16 2021-07-14 Regeneron Pharmaceuticals, Inc. Targeted modification of rat genome
WO2015006725A2 (en) 2013-07-12 2015-01-15 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells from normal human mammary epithelial cells
EP3152295B1 (en) 2014-06-05 2020-07-29 Cedars-Sinai Medical Center A novel and efficient method for reprogramming immortalized lymphoblastoid cell lines to induced pluripotent stem cells
CA2963315A1 (en) 2014-10-15 2016-04-21 Regeneron Pharmaceuticals, Inc. Methods and compositions for generating or maintaining pluripotent cells
US10221395B2 (en) 2016-06-16 2019-03-05 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US11572545B2 (en) 2016-06-16 2023-02-07 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11572545B2 (en) 2016-06-16 2023-02-07 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US11970714B2 (en) 2016-06-16 2024-04-30 Cedars-Sinai Medical Center Method for reprogramming blood to induced pluripotent stem cells

Also Published As

Publication number Publication date
WO2017219000A1 (en) 2017-12-21
US10221395B2 (en) 2019-03-05
US20200370023A1 (en) 2020-11-26
JP2022191394A (ja) 2022-12-27
US10745671B2 (en) 2020-08-18
EP3472304A4 (en) 2019-06-05
US20190136203A1 (en) 2019-05-09
EP3472304B1 (en) 2023-08-09
CN109642212A (zh) 2019-04-16
CN109642212B (zh) 2023-11-03
AU2017286794B2 (en) 2023-07-27
JP7160690B2 (ja) 2022-10-25
EP4265716A2 (en) 2023-10-25
EP4265716A3 (en) 2024-01-24
JP2024116318A (ja) 2024-08-27
KR20190018709A (ko) 2019-02-25
KR102388643B1 (ko) 2022-04-20
EP3472304A1 (en) 2019-04-24
US11970714B2 (en) 2024-04-30
CA3025678A1 (en) 2017-12-21
CN117660311A (zh) 2024-03-08
US20170362574A1 (en) 2017-12-21
AU2023237193A1 (en) 2023-10-26
JP2019524070A (ja) 2019-09-05
AU2017286794A1 (en) 2018-12-13
MX380817B (es) 2025-03-12

Similar Documents

Publication Publication Date Title
MX2018015360A (es) Un metodo eficiente y novedoso para reprogramar la sangre para las celulas madre pluripotentes inducidas.
SG11201808590TA (en) Fibroin-like protein variant and cell culture method
GB2561496A (en) Devices and methods for simulating a function of a liver tissue
CY1121217T1 (el) Μεθοδοι για συγκομιδη καλλιεργειων κυτταρου θηλαστικων
AR092117A1 (es) Composiciones bioactivas y metabolitos de bacillus megaterium
Fotino et al. Re-engineering islet cell transplantation
UY36475A (es) Composiciones y metodos para el uso de insecticida con bacillus sp d747
GB2562396A (en) Devices for simulating a function of a liver tissue and methods of use and manufacturing thereof
MX383334B (es) Celulas discogenicas aisladas, metodos de uso, y metodos de preparacion de las mismas a partir de tejido de mamifero.
MY159971A (en) Multipotent/pluripotent cells and methods
AR082820A1 (es) Diferenciacion de celulas madre embrionarias humanas
EA201890137A1 (ru) Среда для культивирования клеток с добавлением таурина и способы применения
WO2014190361A3 (en) Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna
MX2021005021A (es) Proceso para producir celulas t geneticamente modificadas.
BR112016028512A2 (pt) métodos de cultivo por perfusão e seu uso
MX2017000704A (es) Generacion de un banco de celula estromal mesenquimal a partir de celulas mononucleares agrupadas de multiples donadores de medula osea.
WO2016081032A3 (en) Mesenchymal stem cells derived from induced pluripotent stem cells
EP3805370A4 (en) MESENCHYMATE STEM CELL DERIVED FROM HLA GENE DELETED HUMAN INDUCED PLURIPOTENT STEM CELL AND METHOD FOR PREPARING IT
MY199613A (en) Cell population for transplantation and method for producing same
EP4065684A4 (en) THYMUS ORGANOIDS BIOTECHNOLOGICALLY MANUFACTURED FROM HUMAN PLURIPOTENTS STEM CELLS
EA201791233A1 (ru) Материал стволовых клеток и способ его получения
MX2021001981A (es) Metodos para preparar un poloxamero para usar en medio de cultivo celular.
IL283176A (en) Method for culturing cord blood-derived natural killer cells using transformed t-cells
MX2021013005A (es) Metodos de fabricacion de celulas car-t alogenicas.
EP3508564A4 (en) CELL CULTURE SUBSTRATE, KIT FOR PRODUCING A CELL CULTURE SUBSTRATE AND METHOD FOR PRODUCING GEL / CELL HYBRID TISSUE WITH A CELL CULTURAL SUBSTRATE AND CELL CULTURE SUBSTRATE PRODUCTION KIT