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AR082820A1 - Diferenciacion de celulas madre embrionarias humanas - Google Patents

Diferenciacion de celulas madre embrionarias humanas

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Publication number
AR082820A1
AR082820A1 ARP110103176A ARP110103176A AR082820A1 AR 082820 A1 AR082820 A1 AR 082820A1 AR P110103176 A ARP110103176 A AR P110103176A AR P110103176 A ARP110103176 A AR P110103176A AR 082820 A1 AR082820 A1 AR 082820A1
Authority
AR
Argentina
Prior art keywords
cells
population
differentiation
embryous
human
Prior art date
Application number
ARP110103176A
Other languages
English (en)
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of AR082820A1 publication Critical patent/AR082820A1/es

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0613—Cells from endocrine organs
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/19—Growth and differentiation factors [GDF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Se proporcionan métodos para promover la diferenciación de células madre pluripotentes en células que producen insulina. Particularmente.Reivindicación 1: Una población de células, en donde una cantidad mayor que 85% de las células en la población expresan marcadores característicos del linaje del endodermo definitivo. Reivindicación 2: Un método para generar una población de células, en donde una cantidad mayor que 85% de células en la población expresa marcadores característicos del linaje del endodermo definitivo; el método comprende las etapas de: a) cultivar una población de células madre pluripotentes, b) diferenciar la población de células madre pluripotentes a una población de células, en donde una cantidad mayor que 85% de las células en la población expresan marcadores característicos del linaje del endodermo definitivo en medio sin suero y complementado con BSA y un factor seleccionado del grupo que consiste en insulina e IGF-1.
ARP110103176A 2010-08-31 2011-08-31 Diferenciacion de celulas madre embrionarias humanas AR082820A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37847210P 2010-08-31 2010-08-31

Publications (1)

Publication Number Publication Date
AR082820A1 true AR082820A1 (es) 2013-01-09

Family

ID=45697775

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110103176A AR082820A1 (es) 2010-08-31 2011-08-31 Diferenciacion de celulas madre embrionarias humanas

Country Status (17)

Country Link
US (2) US9528090B2 (es)
EP (3) EP2611910B1 (es)
JP (1) JP6218605B2 (es)
KR (1) KR101836850B1 (es)
CN (2) CN103154239B (es)
AR (1) AR082820A1 (es)
AU (1) AU2011296382B2 (es)
BR (1) BR112013004514A2 (es)
CA (1) CA2809303C (es)
ES (2) ES2659393T3 (es)
HK (1) HK1243729A1 (es)
MX (1) MX355077B (es)
PH (1) PH12013500389A1 (es)
PL (2) PL2853589T3 (es)
RU (1) RU2627168C2 (es)
SG (2) SG10201506852VA (es)
WO (1) WO2012030539A2 (es)

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US20120052575A1 (en) 2012-03-01
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