[go: up one dir, main page]

MX2018005611A - Metodo y aparato para la formacion de imagenes volumetricas de una matriz tridimensional que contiene acido nucleico. - Google Patents

Metodo y aparato para la formacion de imagenes volumetricas de una matriz tridimensional que contiene acido nucleico.

Info

Publication number
MX2018005611A
MX2018005611A MX2018005611A MX2018005611A MX2018005611A MX 2018005611 A MX2018005611 A MX 2018005611A MX 2018005611 A MX2018005611 A MX 2018005611A MX 2018005611 A MX2018005611 A MX 2018005611A MX 2018005611 A MX2018005611 A MX 2018005611A
Authority
MX
Mexico
Prior art keywords
nucleic acid
volumetric imaging
acid containing
containing matrix
dimensional nucleic
Prior art date
Application number
MX2018005611A
Other languages
English (en)
Inventor
George M Crch
R Daugharthy Evan
C Terry Richard
Hyuk Lee Je-
W Pruitt Benjamin
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=58662729&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2018005611(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Harvard College filed Critical Harvard College
Publication of MX2018005611A publication Critical patent/MX2018005611A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2543/00—Reactions characterised by the reaction site, e.g. cell or chromosome
    • C12Q2543/10—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis
    • C12Q2543/101—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis in situ amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/601—Detection means characterised by use of a special device being a microscope, e.g. atomic force microscopy [AFM]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Stereoscopic And Panoramic Photography (AREA)
  • Cell Biology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Se proporcionan métodos para formación de imágenes volumétricas de una matriz tridimensional de ácidos nucleicos dentro de una célula; se proporciona un aparato automatizado para secuenciación e imágenes volumétricas de una matriz tridimensional de ácidos nucleicos.
MX2018005611A 2015-11-03 2016-11-03 Metodo y aparato para la formacion de imagenes volumetricas de una matriz tridimensional que contiene acido nucleico. MX2018005611A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562250182P 2015-11-03 2015-11-03
PCT/US2016/060279 WO2017079406A1 (en) 2015-11-03 2016-11-03 Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix

Publications (1)

Publication Number Publication Date
MX2018005611A true MX2018005611A (es) 2018-11-09

Family

ID=58662729

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018005611A MX2018005611A (es) 2015-11-03 2016-11-03 Metodo y aparato para la formacion de imagenes volumetricas de una matriz tridimensional que contiene acido nucleico.

Country Status (10)

Country Link
US (4) US10266888B2 (es)
EP (1) EP3371329A4 (es)
JP (3) JP6882282B2 (es)
CN (1) CN108474022A (es)
AU (2) AU2016349288A1 (es)
CA (1) CA3004285A1 (es)
GB (1) GB2559526B (es)
HK (1) HK1252878A1 (es)
MX (1) MX2018005611A (es)
WO (1) WO2017079406A1 (es)

Families Citing this family (120)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014531908A (ja) 2011-10-14 2014-12-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 構造アッセンブリによる配列決定
ES2952728T3 (es) 2011-12-22 2023-11-03 Harvard College Métodos para la detección de analitos
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
RU2690935C2 (ru) 2013-06-04 2019-06-06 Президент Энд Фэллоуз Оф Харвард Коллидж Направляемая рнк регуляция транскрипции
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
EP4119677B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN108474029B (zh) 2015-08-07 2021-07-23 麻省理工学院 通过扩展显微法的蛋白质和核酸的纳米级成像
EP3372985B1 (en) 2015-10-28 2025-06-25 The University Of Tokyo Analysis device
JP6882282B2 (ja) * 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
WO2017147528A1 (en) * 2016-02-26 2017-08-31 University Of Southern California Optimized volumetric imaging with selective volume illumination and light field detection
EP4613756A3 (en) 2016-04-25 2025-11-12 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
WO2017222453A1 (en) 2016-06-21 2017-12-28 Hauling Thomas Nucleic acid sequencing
EP3507364A4 (en) 2016-08-31 2020-05-20 President and Fellows of Harvard College METHODS OF GENERATING NUCLEIC ACID SEQUENCE LIBRARIES FOR IN SITU FLUORESCENT SEQUENCING DETECTION
CN109923216B (zh) 2016-08-31 2024-08-02 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
JP6730525B2 (ja) 2016-11-21 2020-07-29 ナノストリング テクノロジーズ，インコーポレイティド 化学組成物とそれを利用する方法
WO2018136856A1 (en) 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
WO2018157048A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for examining podocyte foot processes in human renal samples using conventional optical microscopy
WO2018157074A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for diagnosing neoplastic lesions
JPWO2018199080A1 (ja) * 2017-04-28 2020-03-12 シンクサイト株式会社 イメージングフローサイトメーター
EP3646010A4 (en) * 2017-06-27 2020-11-11 Qcdx, Llc QUANTITATIVE LIQUID BIOPSY DIAGNOSTIC SYSTEM AND METHOD
US11180804B2 (en) 2017-07-25 2021-11-23 Massachusetts Institute Of Technology In situ ATAC sequencing
CN120210336A (zh) 2017-10-06 2025-06-27 10X基因组学有限公司 Rna模板化连接
CN113801784B (zh) * 2018-01-23 2024-07-30 深圳市真迈生物科技有限公司 核酸加载装置及用途
WO2019152670A1 (en) * 2018-01-31 2019-08-08 The Regents Of The University Of California High numerical aperture selective plane illumination microscopy
US11873374B2 (en) 2018-02-06 2024-01-16 Massachusetts Institute Of Technology Swellable and structurally homogenous hydrogels and methods of use thereof
KR20250162921A (ko) 2018-04-09 2025-11-19 더 보드 오브 트러스티스 오브 더 리랜드 스탠포드 쥬니어 유니버시티 계내 유전자 시퀀싱의 방법
CN108485967A (zh) * 2018-04-12 2018-09-04 深圳大学 一种细胞培养装置及应用其检测细胞的方法
EP3794146B1 (en) 2018-05-14 2025-12-10 Bruker Spatial Biology, Inc. Method for identifying a predetermined nucleotide sequence
WO2019241443A1 (en) 2018-06-13 2019-12-19 Thinkcyte Inc. Methods and systems for cytometry
WO2020013833A1 (en) 2018-07-13 2020-01-16 Massachusetts Institute Of Technology Dimethylacrylamide (dmaa) hydrogel for expansion microscopy (exm)
CN112770776B (zh) * 2018-07-30 2025-08-19 瑞德库尔有限责任公司 用于样品处理或分析的方法和系统
CN112703252B (zh) 2018-08-03 2024-09-10 10X基因组学有限公司 用于最小化条形码交换的方法和系统
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
EP3921418A4 (en) 2019-02-06 2023-02-08 Singular Genomics Systems, Inc. Compositions and methods for nucleic acid sequencing
CA3130889A1 (en) 2019-02-22 2020-08-27 Massachusetts Institute Of Technology Iterative direct expansion microscopy
US20240027417A1 (en) * 2019-03-19 2024-01-25 SafetySpect Inc. System and method for assessing biological tissue
CN113906147B (zh) 2019-05-31 2024-09-13 10X基因组学有限公司 检测目标核酸分子的方法
WO2021046135A1 (en) * 2019-09-03 2021-03-11 13.8, Inc. Methods for sequencing nucleic acid molecules
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
CA3155289A1 (en) * 2019-09-23 2021-04-01 Element Biosciences, Inc. Methods for cellularly addressable nucleic acid sequencing
US12265004B2 (en) 2019-11-05 2025-04-01 Massachusetts Institute Of Technology Membrane probes for expansion microscopy
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
JP7773373B2 (ja) * 2019-11-27 2025-11-19 イルミナ インコーポレイテッド オンフローセル三次元ポリマー構造
US11802822B2 (en) 2019-12-05 2023-10-31 Massachusetts Institute Of Technology Multiplexed expansion (MultiExM) pathology
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
EP4083603A4 (en) 2019-12-27 2024-04-03 ThinkCyte, Inc. FLOW CYTOMETER PERFORMANCE EVALUATION METHODS AND STANDARD PARTICLE SUSPENSION
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US12215379B2 (en) 2020-02-17 2025-02-04 10X Genomics, Inc. In situ analysis of chromatin interaction
CN115461143A (zh) * 2020-02-21 2022-12-09 10X基因组学有限公司 用于样品处理的组合物、方法和系统
JP2023514749A (ja) 2020-02-21 2023-04-07 １０エックス ジェノミクス インコーポレイテッド 統合型インサイチュ空間的アッセイのための方法および組成物
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
WO2021200960A1 (ja) 2020-04-01 2021-10-07 シンクサイト株式会社 観察装置
WO2021200911A1 (ja) 2020-04-01 2021-10-07 シンクサイト株式会社 フローサイトメーター
EP4153984A1 (en) 2020-05-19 2023-03-29 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
US12013521B2 (en) 2020-06-29 2024-06-18 Mgi Tech Co., Ltd. Systems and methods for optical scanning and imaging through a fluid medium for nucleic acid sequencing
WO2022032194A1 (en) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
WO2022032195A2 (en) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Spatial sequencing
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
ES2993269T3 (en) 2020-09-18 2024-12-26 10X Genomics Inc Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
US20220195498A1 (en) 2020-12-23 2022-06-23 10X Genomics, Inc. Methods and compositions for analyte detection
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
WO2022164809A1 (en) 2021-01-26 2022-08-04 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
ES2989523T3 (es) 2021-02-19 2024-11-26 10X Genomics Inc Método de uso de un dispositivo modular de soporte para ensayos
JP7591424B2 (ja) * 2021-02-24 2024-11-28 日本放送協会 インコヒーレントホログラム撮像装置
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
WO2022187366A1 (en) 2021-03-03 2022-09-09 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
WO2022235764A1 (en) 2021-05-05 2022-11-10 Singular Genomics Systems, Inc. Multiomic analysis device and methods of use thereof
EP4347878B1 (en) * 2021-05-28 2025-07-16 The Broad Institute, Inc. Co-mapping transcriptional states and protein histology
CN117396613A (zh) 2021-06-01 2024-01-12 10X基因组学有限公司 用于分析物检测和探针解析的方法和组合物
EP4347880A1 (en) 2021-06-02 2024-04-10 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
EP4367507A1 (en) * 2021-07-08 2024-05-15 XEOS Medical NV Specimen receptacle system for an imaging apparatus for imaging ex-vivo tissue specimens
WO2023283442A1 (en) 2021-07-09 2023-01-12 10X Genomics, Inc. Methods for detecting analytes using sparse labelling
CN117651855A (zh) 2021-07-13 2024-03-05 10X基因组学有限公司 用于制备具有可控厚度的聚合基质的方法
WO2023009842A1 (en) 2021-07-30 2023-02-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
CN118043476A (zh) 2021-08-03 2024-05-14 10X基因组学有限公司 核酸多联体及其稳定和/或压缩方法
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
CN117858958A (zh) 2021-08-16 2024-04-09 10X基因组学有限公司 包含分割条形码区的探针和使用方法
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
EP4423296A2 (en) 2021-12-27 2024-09-04 10X Genomics, Inc. Methods and compositions for rolling circle amplification
EP4466376A1 (en) 2022-01-21 2024-11-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
CN118974836A (zh) 2022-03-08 2024-11-15 10X基因组学有限公司 将光学拥挤最小化的原位代码设计方法
WO2023183881A2 (en) 2022-03-24 2023-09-28 Digital Biology Inc. Tissue spatial omics
EP4505177A1 (en) 2022-04-01 2025-02-12 10x Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
EP4504969A1 (en) 2022-04-06 2025-02-12 10X Genomics, Inc. Methods for multiplex cell analysis
US20250197843A1 (en) * 2022-04-08 2025-06-19 University Of Florida Research Foundation, Incorporated Instrument and methods involving high-throughput screening and directed evolution of molecular functions
EP4519674A1 (en) 2022-05-06 2025-03-12 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
EP4519453A1 (en) 2022-05-06 2025-03-12 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
CN119213143A (zh) 2022-05-11 2024-12-27 10X基因组学有限公司 用于原位测序的组合物和方法
CA3257864A1 (en) * 2022-06-06 2023-12-14 Element Biosciences Inc IMAGE SUPERPOSITION IN A PRIMARY ANALYSIS
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2024020395A2 (en) 2022-07-19 2024-01-25 Digital Biology Inc. Barcode diffusion-based spatial omics
WO2024040060A1 (en) 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
EP4578017A1 (en) * 2022-08-23 2025-07-02 Leica Microsystems CMS GmbH Connector, marker, capture construct and data storage device
EP4339297A1 (en) 2022-09-19 2024-03-20 Miltenyi Biotec B.V. & Co. KG Targeted detection of single nucleotide polymorphisms (snp) directly on tissue
KR102844963B1 (ko) * 2022-09-27 2025-08-08 국립부경대학교 산학협력단 광음향 영상 및 초음파 영상의 합성 영상 입력장치 및 그 방법
EP4602344A1 (en) 2022-10-14 2025-08-20 10X Genomics, Inc. Methods for analysis of biological samples
US20240158852A1 (en) 2022-11-16 2024-05-16 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
EP4634404A1 (en) 2022-12-16 2025-10-22 10X Genomics, Inc. Methods and compositions for assessing performance
WO2024137860A1 (en) 2022-12-21 2024-06-27 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
EP4461816A1 (en) 2023-05-10 2024-11-13 10X Genomics, Inc. Boronic acid compositions and methods for tethering ribonucleic acids in biological samples
WO2024249961A1 (en) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
US12319956B2 (en) 2023-07-31 2025-06-03 10X Genomics, Inc. Methods and systems for targeted RNA cleavage and target RNA-primed rolling circle amplification
US20250163502A1 (en) 2023-11-22 2025-05-22 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
WO2025117061A1 (en) * 2023-11-30 2025-06-05 Agilent Technologies, Inc. System and method for water immersion objective bubble detection
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
WO2025239936A1 (en) 2024-05-13 2025-11-20 Element Biosciences, Inc. Multivalent probes and methods of use

Family Cites Families (215)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4123610A (en) 1977-03-09 1978-10-31 The United States Government Nucleic acid crosslinking agent and affinity inactivation of nucleic acids therewith
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US4981985A (en) 1987-10-20 1991-01-01 Trustees Of The University Of Pennsylvania Synthesis of photolabile chelators for multivalent cations
US4886741A (en) 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US4844617A (en) * 1988-01-20 1989-07-04 Tencor Instruments Confocal measuring microscope with automatic focusing
US5151189A (en) 1990-09-17 1992-09-29 Gelman Sciences, Inc. Cationic charge modified microporous membrane
JPH04268359A (ja) 1991-02-22 1992-09-24 Taiho Yakuhin Kogyo Kk 刺激応答性ヒドロゲル及びその製造方法
CA2107683C (en) 1992-02-13 2004-07-20 Dale G. Swan Immobilization of chemical species in crosslinked matrices
US5594235A (en) * 1993-06-17 1997-01-14 Ultrapointe Corporation Automated surface acquisition for a confocal microscope
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5578832A (en) * 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US6284284B1 (en) 1995-06-06 2001-09-04 Advanced Tissue Sciences, Inc. Compositions and methods for production and use of an injectable naturally secreted extracellular matrix
US5830708A (en) 1995-06-06 1998-11-03 Advanced Tissue Sciences, Inc. Methods for production of a naturally secreted extracellular matrix
PL331513A1 (en) 1996-06-06 1999-07-19 Lynx Therapeutics Method of sequencing, by a ligand effect, specific encoded adapters and composition containing double-string oligonucleotidic adapters
JP4532608B2 (ja) 1997-02-07 2010-08-25 オリンパス株式会社 走査型プローブ顕微鏡を用いた核酸検出方法
US5948617A (en) 1997-06-13 1999-09-07 Biospeparations, Inc. Methods of in situ hybridization
US6083726A (en) 1998-02-03 2000-07-04 Lucent Technologies, Inc. Methods for polynucleotide synthesis and articles for polynucleotide hybridization
US6068979A (en) 1998-04-22 2000-05-30 Lumigen, Inc. Simplified sequential chemiluminescent detection
WO1999067641A2 (en) 1998-06-24 1999-12-29 Illumina, Inc. Decoding of array sensors with microspheres
WO2000008212A1 (en) 1998-08-07 2000-02-17 Cellay, Llc Gel microdrops in genetic analysis
US6232067B1 (en) 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
US6632655B1 (en) 1999-02-23 2003-10-14 Caliper Technologies Corp. Manipulation of microparticles in microfluidic systems
US6534266B1 (en) 1999-04-22 2003-03-18 Albert Einstein College Of Medicine Of Yeshiva University Assay of transcription sites by multi-fluor fish
US20020015952A1 (en) 1999-07-30 2002-02-07 Anderson Norman G. Microarrays and their manufacture by slicing
CA2386151A1 (en) 1999-10-12 2001-04-19 Marc Madou Reactive polymeric valve, dispensing devices and methods using same
AU1774501A (en) * 1999-11-17 2001-05-30 Research Foundation Of State University Of New York, The Three dimensional data storage device and method for reading
AU2001247414A1 (en) 2000-03-14 2001-09-24 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
ES2287134T3 (es) 2000-06-02 2007-12-16 Bayer Corporation Procedimiento para la deteccion y localizacion de genes in situ usando hibridacion de adn ramificado.
US8548745B2 (en) * 2000-06-08 2013-10-01 The Regents Of The University Of California Visual-servoing optical microscopy
US7613571B2 (en) 2000-07-28 2009-11-03 Doyle Michael D Method and system for the multidimensional morphological reconstruction of genome expression activity
US6797152B2 (en) 2000-07-31 2004-09-28 California Institute Of Technology Sensors and sensing methods for detecting analytes based on changes in pKa of a sensing polymer
AU2002243263A1 (en) 2000-11-15 2002-07-24 Roche Diagnostics Corporation Methods and reagents for identifying rare fetal cells in the material circulation
AU3090102A (en) 2000-12-14 2002-06-24 Gen Probe Inc Method and kit for enhancing the association rates of polynucleotides
DK1370690T3 (da) 2001-03-16 2012-07-09 Kalim Mir Arrays og fremgangsmåder til anvendelse heraf
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US20060248349A1 (en) 2001-09-24 2006-11-02 Rathjen Alicemarie G Method for preparing and using personal and genetic profiles
AU2002360272A1 (en) 2001-10-10 2003-04-22 Superarray Bioscience Corporation Detecting targets by unique identifier nucleotide tags
GB2382137A (en) 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
EP1451356A1 (en) 2001-11-23 2004-09-01 Simon Fredriksson Method and kit for proximity probing with multivalent proximity probes
US20090246879A1 (en) 2002-12-20 2009-10-01 Callida Genomics Materials and Methods Relating to Nano-Tags and Nano-Brands
CA2513535C (en) 2003-01-29 2012-06-12 454 Corporation Bead emulsion nucleic acid amplification
JP4691014B2 (ja) 2003-02-26 2011-06-01 カリダ ゲノミクス，インコーポレーテッド ハイブリダイゼーションによるランダムアレイｄｎａ分析
US7385043B1 (en) 2003-04-30 2008-06-10 The Public Health Research Institute Of The City Of New York, Inc. Homogeneous multiplex screening assays and kits
US7394592B2 (en) * 2003-05-20 2008-07-01 Lucid, Inc. Confocal microscope for imaging of selected locations of the body of a patient
US7255994B2 (en) 2003-06-10 2007-08-14 Applera Corporation Ligation assay
CN1580283A (zh) * 2003-08-13 2005-02-16 清华大学 一种检测核酸分子的方法
US7193054B2 (en) 2003-08-26 2007-03-20 University Of Rochester Nanofabrication using actin filaments
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
US20080050718A1 (en) 2003-11-14 2008-02-28 Gesteland Raymond F Methods, Articles, and Compositions for Identifying Oligonucleotides
US7381529B2 (en) 2003-12-31 2008-06-03 Intel Corporation Methods and compositions for detecting nucleic acids using scanning probe microscopy and nanocodes
AU2005216549A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US20050191687A1 (en) 2004-02-27 2005-09-01 Tianxin Wang Method for multiplexed analyte detection
WO2006076017A2 (en) 2004-04-30 2006-07-20 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7253947B2 (en) * 2004-10-07 2007-08-07 New York University Portable automated confocal microscope
EP1844162B1 (en) 2005-02-01 2014-10-15 Applied Biosystems, LLC Method for identifying a sequence in a polynucleotide
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
ITRM20050068A1 (it) 2005-02-17 2006-08-18 Istituto Naz Per Le Malattie I Metodo per la rivelazione di acidi nucleici di agenti patogeni batterici o di parassiti nelle urine.
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
US20060234261A1 (en) 2005-03-08 2006-10-19 Pierce Niles A Colorimetric readout of hybridization chain reaction
US8252754B2 (en) 2005-03-21 2012-08-28 The Trustees Of Columbia University In The City Of New York Neuronal pain pathway
US20070020650A1 (en) 2005-04-01 2007-01-25 Avak Kahvejian Methods for detecting proteins
AU2006336403A1 (en) 2005-04-08 2007-08-02 Mycosol, Inc. Stilbazium research assays
CN101238221B (zh) 2005-04-12 2011-11-16 Novia公司名下的现场Rcp公司 新型环形探针及其在鉴定生物分子中的用途
US11561951B2 (en) 2005-05-16 2023-01-24 Panvia Future Technologies, Inc. Multidimensional associative memory and data searching
US7601499B2 (en) 2005-06-06 2009-10-13 454 Life Sciences Corporation Paired end sequencing
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
EP1907571B1 (en) * 2005-06-15 2017-04-26 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
WO2007001986A2 (en) 2005-06-20 2007-01-04 Yuling Luo Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
WO2007045998A2 (en) 2005-07-01 2007-04-26 Dako Denmark A/S New nucleic acid base pairs
US8486621B2 (en) 2005-08-11 2013-07-16 Cornell Research Foundation, Inc. Nucleic acid-based matrixes
CN1959384A (zh) * 2005-11-01 2007-05-09 河南省生物工程技术研究中心 一种三维网格检测技术
EP1954838B1 (en) 2005-11-14 2014-02-26 Life Technologies Corporation Coded molecules for detecting target analytes
JP5700911B2 (ja) 2005-12-23 2015-04-15 ナノストリング テクノロジーズ，インコーポレーテッド 配向され、固定化された巨大分子を含む組成物とその製造法
EP1963531B1 (en) 2005-12-23 2011-09-21 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
US7864996B2 (en) * 2006-02-17 2011-01-04 Lucid, Inc. System for macroscopic and confocal imaging of tissue
CA2647382A1 (en) 2006-03-10 2007-09-20 President And Fellows Of Harvard College Methods and apparatus for near field irradiation
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
WO2007111937A1 (en) 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
SG170802A1 (en) * 2006-03-31 2011-05-30 Solexa Inc Systems and devices for sequence by synthesis analysis
AU2007237909A1 (en) * 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
US7941279B2 (en) 2006-05-22 2011-05-10 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
US7964347B2 (en) 2006-06-15 2011-06-21 Krassen Dimitrov Labels for electronic detection of individual molecules and methods for their detection
WO2007149696A1 (en) * 2006-06-23 2007-12-27 Applera Corporation Systems and methods for cooling in biological analysis instruments
US7745129B1 (en) 2006-07-12 2010-06-29 Kenneth David Schatz Methods for sequencing of a necleic acid
CA2659805A1 (en) 2006-08-04 2008-02-14 Ikonisys, Inc. Dynamic scanning automatic microscope and method
WO2008033848A2 (en) 2006-09-11 2008-03-20 The Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For And On Behalf Of Arizona State University Self-assembled combinatorial encoding nanoarrays for multiplexed biosensing
US20090208965A1 (en) 2006-10-25 2009-08-20 Ikonisys, Inc. Automated method for detecting cancers and high grade hyperplasias
WO2008055915A2 (en) * 2006-11-06 2008-05-15 Clondiag Gmbh Device and process for assays using binding members
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
NZ579002A (en) 2007-03-02 2012-03-30 Danisco Cultures with improved phage resistance
US8145677B2 (en) 2007-03-27 2012-03-27 Faleh Jassem Al-Shameri Automated generation of metadata for mining image and text data
CA2687292C (en) 2007-04-10 2017-07-04 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
US9217151B2 (en) 2007-05-16 2015-12-22 California Institute Of Technology Versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
DK2171088T3 (en) 2007-06-19 2016-01-25 Stratos Genomics Inc Nucleic acid sequencing in a high yield by expansion
WO2009018576A1 (en) 2007-08-02 2009-02-05 Biodesic Compositions and methods for analyte detection and quantitation
WO2009046149A1 (en) 2007-10-01 2009-04-09 Applied Biosystems Inc. Chase ligation sequencing
WO2009046445A1 (en) 2007-10-04 2009-04-09 Halcyon Molecular Sequencing nucleic acid polymers with electron microscopy
US20090139311A1 (en) * 2007-10-05 2009-06-04 Applied Biosystems Inc. Biological Analysis Systems, Devices, and Methods
WO2009065093A2 (en) 2007-11-17 2009-05-22 University Of Medicine And Dentistry Of New Jersey Use of stem cells for wound healing
WO2009137366A2 (en) * 2008-05-06 2009-11-12 Kollmorgen Corporation Genetic sequencer incorporating fluorescence microscopy
CN101586150B (zh) 2008-05-23 2016-09-28 陕西佰美基因股份有限公司 检测探针、通用寡核苷酸芯片及核酸检测方法及其用途
WO2010011961A2 (en) 2008-07-25 2010-01-28 University Of Georgia Research Foundation, Inc. Prokaryotic rnai-like system and methods of use
EP2324045A4 (en) 2008-08-05 2013-04-03 Univ Cornell PHOTOVERNETIC NUCLEIC ACID HYDROGEL
ES2614810T3 (es) 2008-08-14 2017-06-02 Nanostring Technologies, Inc Nanoindicadores estables
EP2324123B1 (en) 2008-09-10 2016-07-20 Rutgers, The State University of New Jersey IMAGING INDIVIDUAL mRNA MOLECULES USING MULTIPLE SINGLY LABELED PROBES
US20100076057A1 (en) 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
US9156010B2 (en) * 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US20100087325A1 (en) * 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
WO2010054108A2 (en) 2008-11-06 2010-05-14 University Of Georgia Research Foundation, Inc. Cas6 polypeptides and methods of use
US8309306B2 (en) 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
EP3290531B1 (en) 2008-12-19 2019-07-24 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
JP4528345B2 (ja) 2009-01-28 2010-08-18 シャープ株式会社 動画再生装置、動画再生方法、動画再生方法をコンピュータで実現するためのプログラム及びそのプログラムを記録した記録媒体
JP5277082B2 (ja) 2009-06-15 2013-08-28 株式会社日立ハイテクノロジーズ 蛍光分析方法
FR2948475A1 (fr) 2009-07-24 2011-01-28 Bionext Procede de caracterisation d'objets tridimensionnels
US8431151B2 (en) 2009-08-06 2013-04-30 Syracuse University Antimicrobial nanostructured hydrogel web containing silver
JP5954876B2 (ja) 2009-10-13 2016-07-20 ナノストリング テクノロジーズ， インコーポレイテッド ナノレポーターによるタンパク質の検出
CN102712955A (zh) 2009-11-03 2012-10-03 Htg分子诊断有限公司 定量核酸酶保护测序(qNPS)
US9089512B2 (en) 2009-12-18 2015-07-28 President And Fellows Of Harvard College Active scaffolds for on-demand drug and cell delivery
US8632976B2 (en) * 2010-01-19 2014-01-21 Agdia Amplification of TRP1 for specific detection of Phytophthora ramorum
EP2516681B1 (en) 2010-02-11 2017-10-18 Nanostring Technologies, Inc Compositions and methods for the detection of preferably small rnas by bridge hybridisation and ligation
US20110257031A1 (en) 2010-02-12 2011-10-20 Life Technologies Corporation Nucleic acid, biomolecule and polymer identifier codes
WO2011112634A2 (en) 2010-03-08 2011-09-15 California Institute Of Technology Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells
US10087431B2 (en) 2010-03-10 2018-10-02 The Regents Of The University Of California Methods of generating nucleic acid fragments
GB201004292D0 (en) 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
PT2556171E (pt) 2010-04-05 2015-12-21 Prognosys Biosciences Inc Ensaios biológicos codificados espacialmente
EA024121B9 (ru) 2010-05-10 2017-01-30 Дзе Реджентс Ов Дзе Юниверсити Ов Калифорния Композиции эндорибонуклеаз и способы их использования
WO2011143583A1 (en) 2010-05-13 2011-11-17 Illumina, Inc. Binding assays for markers
CA2804450C (en) 2010-07-09 2022-10-11 Max Jan van Min 3-d genomic region of interest sequencing strategies
US10669569B2 (en) 2010-10-15 2020-06-02 Navinci Diagnostics Ab Dynamic range methods
ES2648564T3 (es) 2010-10-21 2018-01-04 Advanced Cell Diagnostics, Inc. Método ultrasensible para la detección in situ de ácidos nucleicos
WO2012056440A1 (en) 2010-10-28 2012-05-03 Nanodoc Ltd. COMPOSITIONS AND METHODS FOR ACTIVATING EXPRESSION BY A SPECIFIC ENDOGENOUS miRNA
US20130323729A1 (en) 2010-10-29 2013-12-05 Olink Ab Proximity Ligation Technology for Western Blot Applications
WO2012058638A2 (en) 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
US8859201B2 (en) 2010-11-16 2014-10-14 Nabsys, Inc. Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
CA2827497C (en) 2011-02-15 2014-12-02 Leica Biosystems Newcastle Ltd. Method for localized in situ detection of mrna
JP5687514B2 (ja) * 2011-02-17 2015-03-18 株式会社日立ハイテクノロジーズ 核酸配列解析装置
DK2500436T3 (en) * 2011-03-17 2016-08-29 Pasteur Institut A method, probe and kit for in situ hybridization of DNA and use thereof
US20120252686A1 (en) 2011-03-31 2012-10-04 Good Start Genetics Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8946389B2 (en) 2011-04-25 2015-02-03 University Of Washington Compositions and methods for multiplex biomarker profiling
EP2705161A1 (de) 2011-05-04 2014-03-12 Genovoxx GmbH Nukleosid-triphosphat-konjugate und methoden zu deren anwendung
GB201107863D0 (en) 2011-05-11 2011-06-22 Olink Ab Method and product
GB201108678D0 (en) 2011-05-24 2011-07-06 Olink Ab Multiplexed proximity ligation assay
EP2714938B1 (en) 2011-05-27 2017-11-15 President and Fellows of Harvard College Methods of amplifying whole genome of a single cell
WO2012164565A1 (en) 2011-06-01 2012-12-06 Yeda Research And Development Co. Ltd. Compositions and methods for downregulating prokaryotic genes
PL222511B1 (pl) 2011-06-08 2016-08-31 Międzynarodowy Inst Biologii Molekularnej I Komórkowej W Warszawie Endorybonukleazy dsRNA
JP2014531908A (ja) 2011-10-14 2014-12-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 構造アッセンブリによる配列決定
ES2952728T3 (es) 2011-12-22 2023-11-03 Harvard College Métodos para la detección de analitos
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
CN104284669A (zh) 2012-02-24 2015-01-14 弗雷德哈钦森癌症研究中心 治疗血红蛋白病的组合物和方法
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
BR112014021104B1 (pt) 2012-02-29 2023-03-28 Sangamo Biosciences, Inc Proteína de fusão de ocorrência não natural compreendendo um domínio de ligação de dna de dedo de zinco manipulado que se liga a um gene htt, seu uso, método in vitro de modificação da expressão de um gene htt em uma célula, e método de geração de um sistema modelo para o estudo da doença de huntington
WO2013141680A1 (en) 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
FI3597749T3 (fi) 2012-05-25 2023-10-09 Univ California Menetelmiä ja koostumuksia rna-ohjattua kohde-dna-modifikaatiota varten ja rna-ohjattua transkription modulaatiota varten
SG196730A1 (en) 2012-07-16 2014-02-13 Agency Science Tech & Res Methods for reading data from a storage medium using a reader and storage devices
EP2878671B1 (en) 2012-07-20 2020-06-03 LSI Medience Corporation Photocoupling method using probe containing photoresponsive nucleic acids
EP2880171B1 (en) 2012-08-03 2018-10-03 The Regents of The University of California Methods and compositions for controlling gene expression by rna processing
EP4163617A1 (en) 2012-08-09 2023-04-12 The Board of Trustees of the Leland Stanford Junior University Methods and compositions for preparing biological specimens for microscopic analysis
WO2014028538A2 (en) 2012-08-13 2014-02-20 William Marsh Rice University Multiplexed in situ molecular analyses and programmable molecular probes for regulated signal amplification
US9709791B2 (en) * 2012-08-15 2017-07-18 Lucid, Inc. Systems and methods for imaging tissue
CN102908119A (zh) 2012-09-26 2013-02-06 温州医学院眼视光研究院 一种共焦扫描成像系统及其像差控制方法
KR101656236B1 (ko) 2012-10-23 2016-09-12 주식회사 툴젠 표적 DNA에 특이적인 가이드 RNA 및 Cas 단백질을 암호화하는 핵산 또는 Cas 단백질을 포함하는, 표적 DNA를 절단하기 위한 조성물 및 이의 용도
PT3363902T (pt) 2012-12-06 2019-12-19 Sigma Aldrich Co Llc Modificação e regulação de genoma baseadas em crispr
EP2931898B1 (en) 2012-12-12 2016-03-09 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
EP4234696A3 (en) 2012-12-12 2023-09-06 The Broad Institute Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
KR20150105956A (ko) 2012-12-12 2015-09-18 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작 및 치료적 적용을 위한 시스템, 방법 및 조성물의 전달, 유전자 조작 및 최적화
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
PT2784162E (pt) 2012-12-12 2015-08-27 Broad Inst Inc Engenharia de sistemas, métodos e composições guia otimizadas para a manipulação de sequências
EP4282970A3 (en) 2012-12-17 2024-01-17 President and Fellows of Harvard College Rna-guided human genome engineering
EP3919505B1 (en) 2013-01-16 2023-08-30 Emory University Uses of cas9-nucleic acid complexes
US9411930B2 (en) * 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
AU2014235794A1 (en) 2013-03-14 2015-10-22 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
US9330295B2 (en) 2013-03-15 2016-05-03 Brown University Spatial sequencing/gene expression camera
US20140273230A1 (en) 2013-03-15 2014-09-18 Sigma-Aldrich Co., Llc Crispr-based genome modification and regulation
KR102874079B1 (ko) 2013-03-15 2025-10-22 더 제너럴 하스피탈 코포레이션 Rna-안내 게놈 편집을 위해 특이성을 증가시키기 위한 절단된 안내 rna(tru-grnas)의 이용
US10510435B2 (en) 2013-04-30 2019-12-17 California Institute Of Technology Error correction of multiplex imaging analysis by sequential hybridization
CN110669826B (zh) 2013-04-30 2025-01-07 加州理工学院 通过顺序杂交编条形码的分子多重标记
EP3004349B1 (en) 2013-05-29 2018-03-28 Cellectis S.A. A method for producing precise dna cleavage using cas9 nickase activity
RU2690935C2 (ru) 2013-06-04 2019-06-06 Президент Энд Фэллоуз Оф Харвард Коллидж Направляемая рнк регуляция транскрипции
US20140356956A1 (en) 2013-06-04 2014-12-04 President And Fellows Of Harvard College RNA-Guided Transcriptional Regulation
CN105492611A (zh) 2013-06-17 2016-04-13 布罗德研究所有限公司 用于序列操纵的优化的crispr-cas双切口酶系统、方法以及组合物
US10539772B2 (en) * 2013-10-09 2020-01-21 Howard Hughes Medical Institute Multiview light-sheet microscopy
US10655173B2 (en) * 2013-10-18 2020-05-19 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
JP6286183B2 (ja) * 2013-11-07 2018-02-28 株式会社日立ハイテクノロジーズ 分析装置
EP3080259B1 (en) 2013-12-12 2023-02-01 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
GB201401885D0 (en) 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
JP6456969B2 (ja) 2014-02-21 2019-01-23 マサチューセッツ インスティテュート オブ テクノロジー 膨張顕微鏡法
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
CN113403373B (zh) 2014-11-21 2025-04-11 布鲁克空间生物学公司 无酶且无扩增的测序
CN107209934B (zh) 2014-12-03 2021-03-30 文塔纳医疗系统公司 用于定量分析异质生物标志物分布的方法、系统和装置
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP4119677B1 (en) * 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US9607375B2 (en) 2015-06-03 2017-03-28 Eileen B. Gallagher Biological data annotation and visualization
JP6946292B2 (ja) 2015-08-06 2021-10-06 エイアールシー バイオ リミテッド ライアビリティ カンパニー ゲノム分析のためのシステムおよび方法
CN108474029B (zh) 2015-08-07 2021-07-23 麻省理工学院 通过扩展显微法的蛋白质和核酸的纳米级成像
US20190155835A1 (en) 2015-11-03 2019-05-23 President And Fellows Of Harvard College Systems and Methods for Processing Spatially Related Sequence Data Received from a Sequencing Device
JP6882282B2 (ja) * 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
US10386300B2 (en) 2015-12-21 2019-08-20 Verily Life Sciences Llc Spectrally and spatially multiplexed fluorescent probes for in situ cell labeling
WO2017161251A1 (en) 2016-03-17 2017-09-21 President And Fellows Of Harvard College Methods for detecting and identifying genomic nucleic acids
EP4613756A3 (en) 2016-04-25 2025-11-12 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
IL290679B2 (en) 2016-07-05 2023-10-01 California Inst Of Techn Hybridization chain reaction based on a minimal initiator
CN116064727A (zh) 2016-07-27 2023-05-05 斯坦福大学托管董事会 高度复用荧光成像
WO2018183876A1 (en) 2017-03-31 2018-10-04 Ultivue, Inc. Dna-antigen exchange and amplification

Also Published As

Publication number Publication date
GB2559526B (en) 2021-02-17
EP3371329A4 (en) 2019-06-19
US20230279484A1 (en) 2023-09-07
US20210363579A1 (en) 2021-11-25
WO2017079406A1 (en) 2017-05-11
CN108474022A (zh) 2018-08-31
US10266888B2 (en) 2019-04-23
AU2016349288A1 (en) 2018-05-31
US20180251833A1 (en) 2018-09-06
AU2023202795A1 (en) 2023-05-25
JP6882282B2 (ja) 2021-06-02
CA3004285A1 (en) 2017-05-11
US11118220B2 (en) 2021-09-14
JP2019501635A (ja) 2019-01-24
HK1252878A1 (zh) 2019-06-06
JP2021090439A (ja) 2021-06-17
US20190241950A1 (en) 2019-08-08
GB2559526A (en) 2018-08-08
EP3371329A1 (en) 2018-09-12
US11542554B2 (en) 2023-01-03
JP2023073304A (ja) 2023-05-25
GB201809029D0 (en) 2018-07-18

Similar Documents

Publication Publication Date Title
MX2018005611A (es) Metodo y aparato para la formacion de imagenes volumetricas de una matriz tridimensional que contiene acido nucleico.
ZA202101720B (en) System and method for secondary analysis of nucleotide sequencing data
SG11201803646YA (en) Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
LT3263715T (lt) Pavienių ląstelių transkriptomo analizės būdas
GB201904349D0 (en) Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
EP3277294A4 (en) Method of identifying human compatible t cell receptors specific for an antigenic target
EP3428269A4 (en) MAGNETIC BEADS BASED NUCLEIC ACID EXTRACTION DEVICE AND USE METHOD
EP3564389A4 (en) METHOD FOR OBTAINING NUCLEIC ACIDS FROM FETAL CELLS
EP3317844A4 (en) SYSTEM AND METHOD FOR MONITORING THE PROGRESS OF A LEARNING THROUGH AN EMPIRICAL LEARNING CYCLE
EP3099820A4 (en) Methods of preparing nucleic acids for sequencing
EP3191630A4 (en) Method for constructing sequencing library based on blood sample and usage thereof in determining fetal genetic abnormality
EP3279310A4 (en) Cell culture apparatus and cell culture method
PT3194612T (pt) Métodos para análise genética quantitativa de adn livre de células
EP3249040A4 (en) Cell culture apparatus and cell culture method
EP3093348A4 (en) METHOD FOR IDENTIFYING DESIRED CELL TYPE USING miRNA EXPRESSION AS AN INDICATOR
SG11201802922QA (en) Promoter system inducing expression by 3-hydroxypropionic acid and method for biological production of 3-hydroxypropionic acid using same
SG11201608463WA (en) Method of preparing cells for 3d tissue culture
GB201608060D0 (en) Method of culturing T cells
EP3196293A4 (en) Three-dimensional cell culture system and cell culture method using same
EP2883961B8 (en) A nucleic acid analysis method and apparatus
EP3770272B8 (en) An analysis method and system for analyzing a nucleic acid amplification reaction
EP3248006A4 (en) Method for preparing a biological sample for use in an immunolabeling process
EP3314006A4 (en) Method for assaying for loss of an organism in an aqueous liquid
GB201600176D0 (en) Method of identifying novel protein aggregation inhibitors based on chemical kinetics
HUE067465T2 (hu) Eljárás elõre meghatározott forrásból származó sejtmentes nukleinsavak arányának meghatározására biológiai mintában