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MX2016002616A - Microorganismo para la produccion de metionina con actividad de metionina sintasa y eflujo de metionina mejorados. - Google Patents

Microorganismo para la produccion de metionina con actividad de metionina sintasa y eflujo de metionina mejorados.

Info

Publication number
MX2016002616A
MX2016002616A MX2016002616A MX2016002616A MX2016002616A MX 2016002616 A MX2016002616 A MX 2016002616A MX 2016002616 A MX2016002616 A MX 2016002616A MX 2016002616 A MX2016002616 A MX 2016002616A MX 2016002616 A MX2016002616 A MX 2016002616A
Authority
MX
Mexico
Prior art keywords
methionine
efflux
microorganism
production
improved
Prior art date
Application number
MX2016002616A
Other languages
English (en)
Other versions
MX374056B (es
Inventor
Rainer Figge
Wanda Dischert
Perrine Vasseur
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of MX2016002616A publication Critical patent/MX2016002616A/es
Publication of MX374056B publication Critical patent/MX374056B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01013—Methionine synthase (2.1.1.13)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención está relacionada a un microorganismo recombinante optimizado para la producción de metionina y/o sus derivados, en donde en dicho microorganismo recombinante, la actividad de la metionina sintasa dependiente de cobalamina y el eflujo de metionina son mejorados; la solicitud también está relacionada a un método para optimizar la producción fermentativa de metionina y/o sus derivados, que comprende los pasos de: c. cultivar un microorganismo recombinante en donde en dicho microorganismo, la actividad de la metionina sintasa dependiente de cobalamina y el eflujo de metionina son mejoradas, en un medio de cultivo apropiado que comprende una fuente de carbono fermentable y una fuente de azufre, y d. recuperar la metionina y/o sus derivados del medio de cultivo.
MX2016002616A 2013-08-30 2014-09-01 Microorganismo para la produccion de metionina con actividad de metionina sintasa y eflujo de metionina mejorados. MX374056B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13306185 2013-08-30
PCT/EP2014/068539 WO2015028674A1 (en) 2013-08-30 2014-09-01 Microorganism for methionine production with improved methionine synthase activity and methionine efflux

Publications (2)

Publication Number Publication Date
MX2016002616A true MX2016002616A (es) 2016-06-06
MX374056B MX374056B (es) 2020-07-27

Family

ID=49162096

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016002616A MX374056B (es) 2013-08-30 2014-09-01 Microorganismo para la produccion de metionina con actividad de metionina sintasa y eflujo de metionina mejorados.

Country Status (12)

Country Link
US (1) US10196658B2 (es)
EP (1) EP3039153B1 (es)
JP (1) JP2016529899A (es)
KR (1) KR102270626B1 (es)
CN (1) CN105658803B (es)
BR (1) BR112016004367B1 (es)
CA (1) CA2922597A1 (es)
ES (1) ES2693789T3 (es)
MX (1) MX374056B (es)
PL (1) PL3039153T3 (es)
RU (1) RU2016110763A (es)
WO (1) WO2015028674A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017525379A (ja) * 2014-09-01 2017-09-07 メタボリック エクスプローラー メチオニン排出を改善した発酵によるメチオニン産生の方法及び微生物
CN108026516A (zh) 2015-08-07 2018-05-11 赢创德固赛有限公司 通过发酵的蛋白硫代羧化物依赖性l-甲硫氨酸生产
CN108291243A (zh) 2015-11-27 2018-07-17 赢创德固赛有限公司 生产l-甲硫氨酸的方法
US20180355390A1 (en) 2016-01-08 2018-12-13 Evonik Degussa Gmbh Method to produce L-methionine by a fermentative production
EP3296404A1 (en) 2016-09-15 2018-03-21 Evonik Degussa GmbH Modified microorganism for production of methionine
CN109055289B (zh) * 2018-07-27 2020-10-27 浙江工业大学 一种高产l-甲硫氨酸的重组大肠杆菌及其应用
CN112961807B (zh) * 2021-03-30 2023-01-20 中国科学院成都生物研究所 一种微生物组合物及在促进青稞种子发芽和生长上的应用

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4110641B2 (ja) 1998-11-17 2008-07-02 味の素株式会社 発酵法によるｌ−メチオニンの製造法
JP2000157267A (ja) 1998-11-24 2000-06-13 Ajinomoto Co Inc 変異型ｍｅｔＪ遺伝子及びＬ−メチオニンの製造法
DE19943587A1 (de) 1999-09-11 2001-03-15 Degussa Neue für das dapF-Gen codierende Nukleotidsequenzen
WO2002010209A1 (en) 2000-08-02 2002-02-07 Degussa Ag Nucleotide sequences which code for the meth gene
BR0200350B1 (pt) * 2001-02-13 2014-10-14 Ajinomoto Kk Bactéria transgênica produtora de l-aminoácido pertencente ao gênero escherichia, e, método para produzir l-aminoácido
DE10126164A1 (de) 2001-05-30 2002-12-05 Degussa Für das metD-gen kodierende Nukleotidsequenzen
EP1567549A1 (en) 2002-11-29 2005-08-31 Basf Aktiengesellschaft Methods for the production of methionine
JP2006517796A (ja) 2003-02-18 2006-08-03 メタボリック エクスプローラー 代謝経路の生成または改変を可能とする進化した微生物の生産方法
EP1656454A4 (en) 2003-07-08 2011-06-22 Novus Int Inc PROCESS FOR METHIONINAL RECOVERY
DE10359668A1 (de) 2003-12-18 2005-07-14 Basf Ag Verfahren zur Herstellung von Methionin
DE10359594A1 (de) 2003-12-18 2005-07-28 Basf Ag PEF-TU-Expressionseinheiten
DE102004009454A1 (de) 2004-02-27 2005-09-15 Degussa Ag Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung von rekombinanten Mikroorganismen
WO2005111202A1 (en) 2004-05-12 2005-11-24 Metabolic Explorer Recombinant enzyme with altered feedback sensitivity
KR100651220B1 (ko) 2004-06-29 2006-11-29 씨제이 주식회사 Ｌ-메씨오닌 생산 균주 및 상기 균주를 이용한ｌ-메씨오닌의 생산방법
DE102004035065A1 (de) 2004-07-20 2006-02-16 Basf Ag P-ET-TS-Expressionseinheiten
KR20080028940A (ko) 2005-06-17 2008-04-02 마이크로비아 인코포레이티드 아미노산 및 대사산물의 개선된 생합성 방법
JP4061557B2 (ja) 2005-07-11 2008-03-19 三菱マテリアル株式会社 相変化膜形成用スパッタリングターゲットおよびその製造方法。
JP2009501548A (ja) 2005-07-18 2009-01-22 ビーエーエスエフ ソシエタス・ヨーロピア 微生物におけるメチオニン生産のためのジメチルジスルフィドの使用
BRPI0613662A2 (pt) 2005-07-18 2017-05-09 Basf Ag microorganismo recombinante, e, método para produzir metionina
PL1969130T3 (pl) 2006-01-04 2014-08-29 Evonik Degussa Gmbh Sposób wytwarzania metioniny i jej prekursorów lub sukcynylo homoseryny przy zastosowaniu mikroorganizmów
WO2007135188A2 (en) 2006-05-24 2007-11-29 Evonik Degussa Gmbh Process for the preparation of l-methionine
KR20080061801A (ko) 2006-12-28 2008-07-03 씨제이제일제당 (주) L-메티오닌 생산능을 향상시키는 폴리펩타이드, 상기 폴리펩타이드를 과발현하는 미생물 및 상기 미생물을 이용한 l- 메티오닌 생산방법
BRPI0720967A2 (pt) 2006-12-29 2014-03-11 Evonik Degussa Gmbh Metionina sintases com inibição reduzida de produto.
KR101140906B1 (ko) * 2007-04-11 2012-07-13 씨제이제일제당 (주) 조성물 및 메티오닌 생산방법
WO2009043372A1 (en) 2007-10-02 2009-04-09 Metabolic Explorer Increasing methionine yield
US8163532B2 (en) * 2008-05-28 2012-04-24 Evonik Degussa Gmbh Microorganisms with a reactivation system for cob(I)alamin-dependent methionine synthase
WO2010020290A1 (en) 2008-08-22 2010-02-25 Metabolic Explorer Producing methionine without n-acetyl methionine
WO2011073738A1 (en) 2009-12-14 2011-06-23 Metabolic Explorer Use of inducible promoters in the production of methionine
AR083468A1 (es) 2010-10-25 2013-02-27 Metabolic Explorer Sa Aumento de la disponibilidad de nadph para la produccion de metionina
WO2012090021A1 (en) * 2010-12-30 2012-07-05 Metabolic Explorer Recombinant microorganism for the fermentative production of methionine
EP2479279A1 (de) 2011-01-20 2012-07-25 Evonik Degussa GmbH Verfahren zur fermentativen Herstellung schwefelhaltiger Aminosäuren
AR086790A1 (es) 2011-06-29 2014-01-22 Metabolic Explorer Sa Un microorganismo para la produccion de metionina con importacion de glucosa mejorada
KR101944150B1 (ko) 2012-06-18 2019-01-30 에보니크 데구사 게엠베하 메티오닌의 발효적 생산을 위한 재조합 미생물

Also Published As

Publication number Publication date
MX374056B (es) 2020-07-27
RU2016110763A (ru) 2017-10-05
ES2693789T3 (es) 2018-12-13
EP3039153A1 (en) 2016-07-06
CN105658803B (zh) 2020-06-23
WO2015028674A1 (en) 2015-03-05
BR112016004367B1 (pt) 2022-06-14
US20160177352A1 (en) 2016-06-23
US10196658B2 (en) 2019-02-05
PL3039153T3 (pl) 2019-02-28
EP3039153B1 (en) 2018-08-22
JP2016529899A (ja) 2016-09-29
CA2922597A1 (en) 2015-03-05
BR112016004367A2 (pt) 2017-10-17
KR20160044037A (ko) 2016-04-22
KR102270626B1 (ko) 2021-06-30
CN105658803A (zh) 2016-06-08

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Owner name: EVONIK DEGUSSA GMBH.*

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