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MX2023009109A - Una variante de isopropilmalato sintasa novedosa y un procedimiento de produccion de l-leucina usando la misma. - Google Patents

Una variante de isopropilmalato sintasa novedosa y un procedimiento de produccion de l-leucina usando la misma.

Info

Publication number
MX2023009109A
MX2023009109A MX2023009109A MX2023009109A MX2023009109A MX 2023009109 A MX2023009109 A MX 2023009109A MX 2023009109 A MX2023009109 A MX 2023009109A MX 2023009109 A MX2023009109 A MX 2023009109A MX 2023009109 A MX2023009109 A MX 2023009109A
Authority
MX
Mexico
Prior art keywords
leucine
novel
same
production method
isopropylmalate synthase
Prior art date
Application number
MX2023009109A
Other languages
English (en)
Inventor
Ji Hye Lee
Byeong Cheol Song
Ae Ji Jeon
Jong Hyun Kim
Hye Won Kim
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of MX2023009109A publication Critical patent/MX2023009109A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03013—2-Isopropylmalate synthase (2.3.3.13)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente divulgación se refiere a un polipéptido modificado novedoso que tiene una actividad isopropilmalato sintasa, un polinucleótido que lo codifica, un microorganismo que comprende el polipéptido y un procedimiento de producción de L-leucina mediante el cultivo del microorganismo.
MX2023009109A 2016-12-28 2019-06-27 Una variante de isopropilmalato sintasa novedosa y un procedimiento de produccion de l-leucina usando la misma. MX2023009109A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR20160181343 2016-12-28

Publications (1)

Publication Number Publication Date
MX2023009109A true MX2023009109A (es) 2023-08-09

Family

ID=62710309

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2019007782A MX2019007782A (es) 2016-12-28 2017-10-20 Una variante de isopropilmalato sintasa novedosa y un procedimiento de producción de l-leucina usando la misma.
MX2023009109A MX2023009109A (es) 2016-12-28 2019-06-27 Una variante de isopropilmalato sintasa novedosa y un procedimiento de produccion de l-leucina usando la misma.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2019007782A MX2019007782A (es) 2016-12-28 2017-10-20 Una variante de isopropilmalato sintasa novedosa y un procedimiento de producción de l-leucina usando la misma.

Country Status (12)

Country Link
US (2) US11104924B2 (es)
EP (2) EP3858987B1 (es)
JP (2) JP6848067B2 (es)
KR (2) KR102055874B1 (es)
CN (2) CN108884449B (es)
CA (2) CA3048802C (es)
ES (2) ES2896257T3 (es)
HU (2) HUE060328T2 (es)
MX (2) MX2019007782A (es)
MY (1) MY198579A (es)
PL (2) PL3858987T3 (es)
WO (1) WO2018124440A2 (es)

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CN108884449B (zh) * 2016-12-28 2022-01-25 Cj第一制糖株式会社 新型异丙基苹果酸合酶变异体及使用其生产l-亮氨酸的方法
CN109456987B (zh) * 2018-10-26 2021-05-25 天津科技大学 高产l-亮氨酸的相关基因及工程菌构建方法与应用
KR102134418B1 (ko) * 2019-06-17 2020-07-16 씨제이제일제당 주식회사 L-타이로신을 생산하는 미생물 및 이를 이용한 l-타이로신 생산 방법
CN110540976B (zh) * 2019-08-29 2020-07-24 天津科技大学 一种异丙基苹果酸合成酶及其应用
WO2021037190A1 (zh) 2019-08-29 2021-03-04 天津科技大学 一种2-异丙基苹果酸合成酶及其工程菌与应用
KR102153534B1 (ko) 2019-09-02 2020-09-09 씨제이제일제당 주식회사 신규한 프로모터 및 이를 이용한 아미노산 생산 방법
KR102143964B1 (ko) * 2019-12-06 2020-08-12 씨제이제일제당 주식회사 신규한 분지쇄 아미노산 아미노트랜스퍼라제 변이체 및 이를 이용한 류신 생산방법
KR102207867B1 (ko) 2020-01-21 2021-01-26 씨제이제일제당 주식회사 Nadp 의존적 글리세르알데하이드-3-포스페이트 디하이드로지나제를 포함하는 미생물을 이용하여 ｌ-아미노산을 생산하는 방법
KR102285951B1 (ko) * 2020-01-30 2021-08-04 씨제이제일제당 주식회사 시트레이트 신타아제의 활성이 약화된 신규한 변이형 폴리펩티드 및 이를 이용한 l-아미노산 생산 방법
KR102360900B1 (ko) * 2020-05-20 2022-02-09 씨제이제일제당 (주) 신규한 폴리펩티드 및 이를 이용한 l-류신의 생산 방법
KR102495918B1 (ko) 2021-01-26 2023-02-06 씨제이제일제당 주식회사 aroG 알돌라아제 (Phospho-2-dehydro-3-deoxyheptonate aldolase) 변이체 및 이를 이용한 분지쇄 아미노산 생산 방법
KR102527096B1 (ko) 2021-02-01 2023-04-28 씨제이제일제당 주식회사 프리페네이트 탈수 효소 (Prephenate dehydratase) 변이체 및 이를 이용한 분지쇄 아미노산 생산 방법
KR102527102B1 (ko) * 2021-03-05 2023-04-28 씨제이제일제당 주식회사 이소프로필말레이트 신타제 변이체 및 이를 이용한 l-류신의 생산 방법
JP2024075803A (ja) * 2021-03-29 2024-06-05 ＧｒｅｅｎＥａｒｔｈＩｎｓｔｉｔｕｔｅ株式会社 改変型α－イソプロピルマレートシンターゼ
KR102281370B1 (ko) * 2021-04-07 2021-07-22 씨제이제일제당 (주) 신규한 2-이소프로필말레이트합성효소 변이체 및 이를 이용한 l-발린 생산 방법
KR20240139382A (ko) 2023-03-14 2024-09-23 씨제이제일제당 (주) 여과 유속이 개선된 아미노산의 제조방법

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KR101796830B1 (ko) * 2015-08-25 2017-11-13 씨제이제일제당 (주) Ｌ-류신 생산능을 가지는 미생물 및 이를 이용한 ｌ-류신의 제조방법
CN106190921B (zh) * 2016-08-08 2019-07-26 天津科技大学 一种谷氨酸棒状杆菌与应用
CN108884449B (zh) 2016-12-28 2022-01-25 Cj第一制糖株式会社 新型异丙基苹果酸合酶变异体及使用其生产l-亮氨酸的方法
CN106754807B (zh) * 2016-12-29 2020-10-30 廊坊梅花生物技术开发有限公司 生产l-亮氨酸菌株和生产l-亮氨酸的方法

Also Published As

Publication number Publication date
KR102055874B1 (ko) 2019-12-13
PL3564366T3 (pl) 2022-01-03
CA3048802A1 (en) 2018-07-05
JP2020503045A (ja) 2020-01-30
ES2896257T3 (es) 2022-02-24
HUE056931T2 (hu) 2022-03-28
US20200032305A1 (en) 2020-01-30
CA3048802C (en) 2021-10-19
BR112019013521A2 (pt) 2020-02-04
EP3858987A1 (en) 2021-08-04
US11104924B2 (en) 2021-08-31
ES2932086T3 (es) 2023-01-11
CN114085800A (zh) 2022-02-25
JP2021019596A (ja) 2021-02-18
EP3858987B1 (en) 2022-11-09
JP6848067B2 (ja) 2021-03-24
CN108884449A (zh) 2018-11-23
PL3858987T3 (pl) 2023-05-22
MY198579A (en) 2023-09-06
WO2018124440A2 (ko) 2018-07-05
KR20180077008A (ko) 2018-07-06
WO2018124440A3 (ko) 2018-08-23
US20210254111A1 (en) 2021-08-19
KR20190058413A (ko) 2019-05-29
EP3564366B1 (en) 2021-09-29
JP7217250B2 (ja) 2023-02-02
EP3564366A4 (en) 2020-08-05
CA3095659A1 (en) 2018-07-05
MX2019007782A (es) 2019-08-29
CN108884449B (zh) 2022-01-25
CA3095659C (en) 2022-12-06
KR102094875B1 (ko) 2020-03-31
HK1258202A1 (zh) 2019-11-08
US11905539B2 (en) 2024-02-20
EP3564366A2 (en) 2019-11-06
HUE060328T2 (hu) 2023-02-28

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