WO2025210149A1 - Fungicidal composition - Google Patents

Abstract

The present invention relates to a composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces chrestomyceticus and component (B) is a compound selected from the group consisting of prothioconazole, difenoconazole, mefentrifluconazole, hexaconazole, propiconazole, bromuconazole, fenpropidin, spiroxamine, isoprothiolane, pydiflumetofen, benzovindiflupyr, isoflucypram, isopyrazam, fluxapyroxad, cyprodinil, azoxystrobin, pyraclostrobin, metyltetraprole, proquinazid, tricyclazole, florylpicoxamid, fenpicoxamid, N-(2,4-dimethyl-1-phenylpentan-2- yl)-8-fluoroquinoline-3-carboxamide, ipflufenoquin, quinofumelin, zinc thiazole, folpet, chlorothalonil, acibenzolar-S-methyl, fosetyl- aluminium, oxolinic acid, anisiflupurin, inpyrfluxam, metalaxyl, metalaxyl-M, triticonazole, penthiopyrad, sedaxane, thiabendazole, imidacloprid, thiamethoxam, fludioxonil, fluoxastrobin, silthiofam, chlorantraniliprole, ethaboxam, ipconazole, cyantraniliprole, oxathiapiprolin, fluopyram, picarbutrazox, cyclobutrifluram, isocycloseram, and fluoxapiprolin. The invention further relates to a method of controlling or preventing phytopathogenic diseases, preferably phytopathogenic fungi, on a plant or on plant propagation material and / or on harvested food crops, which comprises applying to the plant, on plant propagation material, the locus thereof, and / or on harvested food crops the composition according to the present invention.

Description

FUNGICIDAL COMPOSITION

The present invention relates to novel compositions, to their use in agriculture or horticulture for controlling diseases caused by phytopathogens, especially phytopathogenic fungi and / or oomycetes, and to methods of controlling diseases on useful plants.

BACKGROUND

Whilst many compounds and compositions, belonging to various different chemical classes, or which are from biological origin, for instance bacterial origin, have been/are being developed for use as pesticides in crops of useful plants, crop tolerance and activity against pests, such as phytopathogenic fungi do not always satisfy the needs of agricultural practice in many respects. Therefore, there is a continuing need to find new compounds and compositions having superior biological properties for use in controlling or preventing infestation of plants by phytopathogenic pests such as phytopathogenic microorganisms. For example, compounds or compositions possessing a greater biological activity, an advantageous spectrum of activity, an increased safety profile, improved physico-chemical properties, or increased biodegradability. Or else, compositions possessing a broader spectrum of activity, improved crop tolerance, synergistic interactions or potentiating properties, or compositions which display a more rapid onset of action or which have longer lasting residual activity or which enable a reduction in the number of applications and/or a reduction in the application rate of compounds and compositions required for effective control of a phytopathogen, thereby enabling beneficial resistance-management practices, reduced environmental impact and reduced operator exposure. The use of compositions comprising mixtures of different fungicidal compounds possessing different modes of action can address some of these needs (eg, by combining fungicides with differing spectra of activity).

Fungicidal compositions from bacterial origin such as from Streptomyces are for instance disclosed in W02022/038180 and in Rahila et al, (2023) Current Microbiology, 80: 107. In US 5,356,624 a Streptomyces rimosus strain is disclosed that was found active against several wood-degrading fungi. There is a need for mixtures of a composition comprising Streptomyces and a second composition comprising another pesticide, which can solve some of the problems outlined above.

SUMMARY

The present invention relates to a composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces chrestomyceticus., and component (B) comprises a compound selected from the group consisting of prothioconazole, difenoconazole, mefentrifluconazole, hexaconazole, propiconazole, bromuconazole, fenpropidin, spiroxamine, isoprothiolane, pydiflumetofen, benzovindiflupyr, isoflucypram, isopyrazam, fluxapyroxad, cyprodinil, azoxystrobin, pyraclostrobin, metyltetraprole, proquinazid, tricyclazole, florylpicoxamid, fenpicoxamid, feneptamidoquin, N-(2,4-dimethyl-1-phenylpentan-2-yl)-8-fluoroquinoline-3-carboxamide, ipflufenoquin, quinofumelin, zinc thiazole, folpet, chlorothalonil, , fosetyl-aluminium, oxolinic acid, anisiflupurin, inpyrfluxam, metalaxyl, metalaxyl-M, triticonazole, penthiopyrad, sedaxane, thiabendazole, imidacloprid, thiamethoxam, fludioxonil, fluoxastrobin, silthiofam, chlorantraniliprole, ethaboxam, ipconazole, cyantraniliprole, oxathiapiprolin, fluopyram, picarbutrazox, cyclobutrifluram, isocycloseram, and fluoxapiprolin

In a second aspect the present invention relates to a method of controlling or preventing phytopathogenic diseases, preferably phytopathogenic fungi, on a plant or on plant propagation material and / or on harvested food crops, which comprises applying to the plant, on plant propagation material, the locus thereof, and / or on harvested food crops the composition according to the present invention.

In a third aspect the present invention relates to a plant, plant propagation material or the locus thereof or a harvested food crop comprising a composition according to the present invention.

DETAILED DESCRIPTION

The present invention relates to a composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces chrestomyceticus, and component (B) comprises, or is, a compound selected from the group consisting of prothioconazole, difenoconazole, mefentrifluconazole, hexaconazole, propiconazole, bromuconazole, fenpropidin, spiroxamine, isoprothiolane, pydiflumetofen, benzovindiflupyr, isoflucypram, isopyrazam, fluxapyroxad, cyprodinil, azoxystrobin, pyraclostrobin, metyltetraprole, proquinazid, tricyclazole, florylpicoxamid, fenpicoxamid, feneptamidoquin, N-(2,4-dimethyl-1-phenylpentan-2-yl)-8-fluoroquinoline-3-carboxamide, ipflufenoquin, quinofumelin, zinc thiazole, folpet, chlorothalonil, fosetyl-aluminium, oxolinic acid, anisiflupurin, inpyrfluxam, metalaxyl, metalaxyl-M, triticonazole, penthiopyrad, sedaxane, thiabendazole, imidacloprid, thiamethoxam, fludioxonil, fluoxastrobin, silthiofam, chlorantraniliprole, ethaboxam, ipconazole, cyantraniliprole, oxathiapiprolin, fluopyram, picarbutrazox, cyclobutrifluram, isocycloseram, and fluoxapiprolin.

In one embodiment the present invention relates to a composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces chrestomyceticus. and component (B) comprises, or is, a compound selected from the group consisting anisiflupurin, azoxystrobin, benzovindiflupyr, bromuconazole, chlorantraniliprole, chlorothalonil, cyantraniliprole, cyclobutrifluram, cyprodinil, difenoconazole, ethaboxam, fenpicoxamid, fenpropidin, florylpicoxamid, fludioxonil, fluopyram, fluoxapiprolin, fluoxastrobin, fluxapyroxad, folpet, fosetyl-Aluminium, imidacloprid, inpyrfluxam, ipconazole, ipflufenoquin, isocycloseram, isoflucypram, mefentrifluconazole, metalaxyl, metalaxyl-M, metyltetraprole, feneptamidoquin, N-(2,4-dimethyl-1-phenylpentan-2-yl)-8-fluoroquinoline-3-carboxamide, oxathiapiprolin, oxolinicacid, penthiopyrad, picarbutrazox, proquinazid, prothioconazole, pydiflumetofen, pyraclostrobin, quinofumelin, sedaxane, silthiofam, spiroxamine, thiabendazole, thiamethoxam, tricyclazole, triticonazole, and zinc thiazole.

Surprisingly, it was found that a composition according to the present invention provided effective control against phytopathogenic microorganisms, preferably against phytopathogenic fungi. It was surprisingly found that the chemical compounds of component (B) did not affect the activity of the Streptomyces chrestomyceticus in component (A). The benefits provided by certain compositions according to the invention may also include, inter alia, advantageous levels of biological activity for protecting plants against diseases that are caused by pests, for instance fungi, or superior properties for use as agricultural active ingredients (for example, greater biological activity, an advantageous spectrum of activity, an increased safety profile, improved physico-chemical properties, or increased biodegradability).

A composition according to the present invention typically is a pesticidal composition.

A composition, microorganism, metabolite or compound having “pesticidal activity” or “pesticide” as used herein means a composition, microorganism, metabolite or compound that controls, modifies, or prevents the growth of pests. The term “pesticidally effective amount” where used means the quantity of such a composition, microbial strain, compound, or metabolite that is capable of producing an effect on the growth of pests. Controlling or modifying effects include all deviation from natural development, such as killing, retardation and the like, and prevention includes barrier or other defensive formation in or on a plant to prevent pest infection. The term “pesticidal activity” or “pesticides” includes insecticidal or fungicidal activity or “insecticides” or “fungicides”. The composition according to the present invention may also have activity against oomycetes.

Component (B) comprises compounds that may further comprise insecticidal activity, for instance thiamethoxam, imidacloprid, cyantraniliprole, or isocycloseram.

Component (B) comprises compounds that may further comprise activity as plant elicitor such as acibenzolar-s-methyl.

A composition according to the present invention typically is a fungicidal composition. The term “fungicide” or “fungicidal” as used herein means a composition, metabolite or microorganism or compound that controls, modifies, or prevents the growth of fungi. The term “fungicidally effective amount” means the quantity of such a compound, metabolite, microorganism or composition that is capable of producing an effect on the growth of fungi. Controlling or modifying effects include all deviation from natural development, such as killing, retardation and the like, and prevention includes barrier or other defensive formation in or on a plant to prevent fungal infection, eg the composition according to the present invention may act as a plant growth regulator. The composition according to the present invention comprises a fungicidally effective amount of the component (A) and/or the component (B).

The composition according to the present invention typically is an agricultural-acceptable composition.

Throughout this document the expression “composition” stands for the various mixtures or combinations of components (A) and (B) (including the above-defined embodiments), for example in a single “ready-mix” form, in a combined spray mixture composed from separate formulations of the single active ingredient components, such as a “tank-mix”, and in a combined use of the single active ingredients when applied in a sequential manner, i.e. one after the other with a reasonably short period, such as a few hours or days. The order of applying the components (A) and (B) is not essential for working the present invention.

In a preferred embodiment, component (A) comprises a Streptomyces chrestomyceticus. The Streptomyces chrestomyceticus comprises a nucleotide sequence which has at least 99.8 %, preferably at least 99.9%,, preferably at least 99.91%, 99.92%, 99.93%, 99.94%, 99.95%, 99.96%, 99.97%, 99.98%, or 99.99% identity to SEQ ID NO: 1. In one embodiment the Streptomyces comprises a nucleotide sequence according to SEQ ID NO:1. SEQ ID NO: 1 comprises the 16S RNA gene of Streptomyces sp. Saigon413 deposited with the Westerdijk Institute under accession number CBS149411

In another preferred embodiment the component (A) comprises a Streptomyces chrestomyceticus which comprises a genome sequence which has at least 95% identity, preferably at least 96%, 97%, 98%, 99% identity to the whole genome of Streptomyces sp. Saigon413 deposited with the Westerdijk Institute under accession number CBS149411 . The Streptomyces may comprise a genome sequence which has at least 99.1%, 99.2%, 99.3%. 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9% identity to the whole genome of Streptomyces sp. Saigon413 deposited with the Westerdijk Institute under accession number CBS149411 . Preferably component A comprises a Streptomyces chrestomyceticus, which is Streptomyces sp. Saigon413 deposited with the Westerdijk Institute under accession number CBS149411.

As used herein, the terms "percent identity," and "percent identical" refer to the relatedness of two or more nucleotide or amino acid sequences, which may be calculated by (i) comparing two optimally aligned sequences over a window of comparison, (ii) determining the number of positions at which the identical nucleic acid base (for nucleotide sequences) or amino acid residue (for proteins) occurs in both sequences to yield the number of matched positions, (iii) dividing the number of matched positions by the total number of positions in the window of comparison, and then (iv) multiplying this quotient by 100 percent to yield the percent identity. If the "percent identity" is being calculated in relation to a reference sequence without a particular comparison window being specified, then the percent identity is determined by dividing the number of matched positions over the region of alignment by the total length of the reference sequence. Accordingly, for purposes of the present invention, when two sequences (query and subject) are optimally aligned (with allowance for gaps in their alignment), the "percent identity" for the query sequence is equal to the number of identical positions between the two sequences divided by the total number of positions in the query sequence over its length (or a comparison window), which is then multiplied by 100 percent.

The component (A) comprising Streptomyces chrestomyceticus as disclosed herein comprises a cell count of the Streptomyces from 1*10° to 1*10¹⁴ cfu / g dry weight, for instance from1*10¹ to 1*10¹³ cfu / g dry weight, 1*10² to 1*10¹³ cfu / g dry weight, for instance from 1*10³ to 1*10¹² cfu / g dry weight, from 2*10³ to 2*10¹¹ cfu / g dry weight, from 5*10³ to 5*10¹¹ cfu / g dry weight, for instance from 1*10⁴ to 1*10¹° cfu / g dry weight, from 2*10⁴ to 2*10¹° cfu / g dry weight, such as from 1*10⁵ to 1*10⁹ cfu / g dry weight, from 2*10⁵ to 2*10⁹ cfu / g dry weight, from 5*10⁵ to 5*10⁹ cfu / g dry weight, from 1 *10⁶ to 1 *10⁸ cfu / g dry weight, such as from 2*10⁶ to 2*10⁸ cfu / g dry weight.

The component (A) comprising a Streptomyces chrestomyceticus as disclosed herein comprises a fermentation broth comprising the Streptomyces chrestomyceticus, preferably a spray-dried fermentation broth or a freeze-dried fermentation broth. Spray-drying or freeze-drying of a fermentation broth is known in the art. Component (A) comprising a Streptomyces as disclosed herein also includes a formulation comprising the Streptomyces.

A Streptomyces, such as Streptomyces chrestomyceticus, may be cultivated in a suitable fermentation medium under suitable fermentation conditions, and optionally comprising a step of recovering the Streptomyces strain. Usually, a fermentation broth is produced during cultivation of or when cultivating a strain of Streptomyces. Suitable fermentation conditions for cultivating Streptomyces sp. are known to a person skilled in art.

Cultivating a strain of Streptomyces, for instance Streptomyces chrestomyceticus strain, as disclosed herein, comprises cultivating the microbial strain under aerobic conditions at a temperature of from 15 degrees Celsius to 45 degrees Celsius, preferably a temperature of from 20 to 35 degree Celsius, preferably a temperature of between 25 to 32 degrees Celsius, in the presence of a carbon source and a nitrogen source. A suitable carbon source may be molasses, such as beet or cane molasses, polysaccharides, flour, starch, sugar, or glucose. A suitable nitrogen source may be casein hydrolysate, tryptone, ammonium sulphate, ammonia, yeast extract, peptone or urea. The process for cultivating a Streptomyces as disclosed herein may be performed in a batch, fed-batch or continuous culture.

Component (A) as disclosed herein also includes a formulation comprising the Streptomyces chrestomyceticus and / or a fermentation product thereof. Formulations of microbial strains are known in the art for instance as disclosed in Croda Crop Care, the Nouryon formulator toolbox and in: Formulation of Microbial Biopesticides: Beneficial microorganisms, nematodes and seed treatments (412 p., 6 December 2012) eds. Burges H.D., Springer, ISBN 978-94-011-4926-6.

Component (A) comprising a Streptomyces chrestomyceticus as disclosed herein comprises a formulation comprising the Streptomyces chrestomyceticus, wherein the formulation is an oil dispersion (CD), a non-aqueous dispersion (NAD) or a flowable formulation.

Component (A) comprising a Streptomyces chrestomyceticus. as disclosed herein includes a fermentation broth comprising Streptomyces chrestomyceticus.

In one embodiment component (A) further comprises a metabolite. A metabolite is preferably produced by the Streptomyces chrestomyceticus disclosed herein.

In one embodiment, component (A) further comprises a metabolite, wherein the metabolite is malonomicin. Malonomicin (sometimes spelt ‘malonomycin’) is {[(2S)-2-amino-3-hydroxypropanoyl]amino} {2-[(5S)-5-(aminomethyl)-4-hydroxy-2-oxo-2,5-dihydro-1 H-pyrrol-3-yl]-2-oxoethyl}malonic acid can be produced as disclosed in Example I of W02006/078939. Malonomicin may also be prepared according to the method disclosed in Example I A and B in EP 1860939, or according to Law et al, 2018 (Nature Catalys is | VOL 1 | DECEMBER 2018 | 977-984).

Component (A) may further comprise a metabolite, such as at least one, at least two, at least three, at least four or at least five of the metabolite(s), selected from the group consisting of cyclothiazomycin C, streptimidone, an oligosaccharide compound which comprises a molecular formula according to C53H90N2O44, further characterised by the NMR spectra listed in Table 1 and Table 2, further characterized by a structural Formula I, Formula (I), a lipopeptide according to Formula II, or a salt thereof wherein R1 = CH3 or C2H5

Formula (II) and a polyene compound characterized by a molecular formula according to C67H115NO25, wherein the polyene is further characterized by a spectrum of light absorption with absorbance maxima at a wavelength of 235.5 nm, 301.1 nm, 315.8 nm, 330.9 nm and 348.3 nm when measured in an aqueous acetonitrile solution. The polyene is further characterized by the spectrum of light absorption as shown in Figure 10.

For instance, component (A) may comprise a further metabolite wherein the metabolite is selected from the group consisting of cyclothiazomycin C, streptimidone, an oligosaccharide compound which comprises a molecular formula according to C53H90N2O44, further characterised by the NMR spectra listed in Table 1 and Table 2, further characterized by a structural Formula I, Formula (I), and a lipopeptide according to Formula II, or a salt thereof wherein R1 = CH3 or C2H5

Formula (II)

Cyclothiazomycin C is a known compound. The structure of cyclothazomycin C is disclosed on p.

3 of WO2015191789 and can be produced as disclosed in Example 4 of WO2015/191789.

Streptimidone is a known compound of Formula III

Kiyota, H. Synthesis and antifungal activity of the four stereoisomers of streptimidone, a glutarimide antibiotic from Streptomyces rimosus forma paromomycinus. Eur. J. Org. Chem. (20), 3459-3462 (2000).

The metabolites disclosed herein above are preferably produced by the Streptomyces chrestomyceticus in component (A) as disclosed herein. Suitably, component (A) comprises a composition comprising a Streptomyces chrestomyceticus and a metabolite disclosed herein, preferably a metabolite which can be produced by the Streptomyces chrestomyceticus.

The composition as disclosed herein comprises a component (B), wherein component (B) preferably comprises a compound selected from the group consisting of acibenzolar-S-methyl, azoxystrobin, benzovindiflupyr, bromuconazole, chlorothalonil, cyprodinil, difenoconazole, fenpicoxamid, fenpropidin, florylpicoxamid, folpet, isoflucypram, mefentrifluconazole, metyltetraprole, proquinazid, prothioconazole, pydiflumetofen, pyraclostrobin and spiroxamine .

Certain compositions comprising a component (A) and a component (B) as disclosed herein may show a synergistic effect. This occurs whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components. The action to be expected E for a given active ingredient combination obeys the so-called COLBY formula and can be calculated as follows (COLBY, S.R. "Calculating synergistic and antagonistic responses of herbicide combination". Weeds, Vol. 15, pages 20- 22; 1967): ppm = milligrams of active ingredient (= a.i.) per liter of spray mixture

X = % action by active ingredient A) using p ppm of active ingredient

Y = % action by active ingredient B) using q ppm of active ingredient.

According to COLBY, the expected (additive) action of active ingredients A)+B) using p+q ppm of active ingredient is:

If the action actually observed (O) is greater than the expected action (E), then the action of the combination is super-additive, i.e. there is a synergistic effect. In mathematical terms, synergism corresponds to a positive value for the difference of (O-E). In the case of purely complementary addition of activities (expected activity), said difference (O-E) is zero. A negative value of said difference (O-E) signals a loss of activity compared to the expected activity.

It was surprisingly found that a composition as disclosed herein comprising a component (B) which comprises a compound selected from the group consisting of acibenzolar-S-methyl, azoxystrobin, benzovindiflupyr, bromuconazole, chlorothalonil, cyprodinil, difenoconazole, fenpicoxamid, fenpropidin, florylpicoxamid, folpet, isoflucypram, mefentrifluconazole, metyltetraprole, proquinazid, prothioconazole, pydiflumetofen, pyraclostrobin and spiroxamine resulted in a surprising synergistic activity against phytopathogenic fungi.

A composition comprising components (A) and (B) as active ingredients according to the present invention showed synergistic activity against phytopathogenic microorganisms, such as phytopathogenic fungi, such as Zymoseptoria (syn. Septoria) tritici and Puccinia recondita f. sp. tritici (Puccinia triticina) and Pyricularia oryzae. Zymoseptoria tritici and Puccinia recondita f. sp. tritici were affected by a composition according to the present invention on wheat. Pyricularia oryzae was affected by a composition according to the present invention on rice.

Components (A) and (B) as active ingredient may be used at any suitable ratio. Preferably, a composition of the present invention comprises a component (A) and component (B) at a weight ratio of component (A) to component (B) of from 400:1 to 1 :150, for instance from 300:1 to 1 :100, for instance a weight ratio of component (A) to component (B) of from 250:1 to 1 :50, for instance a weight ratio of component (A) to component (B) of from 200:1 to 1 :25, for instance a weight ratio of component (A) to component (B) of from 100:1 to 1 :10, for instance a weight ratio of component (A) to component (B) of from 90:1 to 1 :9, for instance a weight ratio of component A to component B of from 80:1 to 1 :8, for instance a weight ratio of component (A) to component (B) of from 70:1 to 1 :7, for instance a weight ratio of component (A) to component (B) of from 60:1 to 1 :6, for instance a weight ratio of component (A) to component (B) of from 50:1 to 1 :5, for instance a weight ratio of component (A) to component (B) of from 40:1 to 1 :4, for instance a weight ratio of component (A) to component (B) of from 30:1 to 1 :3, for instance a weight ratio of component (A) to component (B) of from 20:1 to 1 :2, for instance a weight ratio of component (A) to component (B) of from 10:1 to 1 :1. A preferred ratio of component (A) to component (B) usually depends on the compounds in component (B).

In one embodiment a composition comprising components (A) and (B) as active ingredients, is a composition wherein component (A) comprises a Streptomyces chrestomyceticusas disclosed herein above, and wherein component (B) comprises prothioconazole at a weight ratio of 100:1 to 1 :50, preferably at a weight ratio of 80:1 to 1 :20, preferably at a weight ratio of 40:1 to 1 : 10. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici.

In one embodiment a composition comprising components (A) and (B) as active ingredients, is a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises difenoconazole at a weight ration of 200:1 to 1 :20, preferably a weight ratio of 150:1 to 1 : 10. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici, Puccinia recondita f. sp. tritici, and Pyricularia oryzae.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, is a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises mefentrifluconazole at a weight ratio of 200:1 to 1 :20, preferably a weight ratio of 150:1 to 1 : 10. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici.

In one embodiment a composition comprising components (A) and (B) as active ingredients, is a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises bromuconazole at a weight ration of 20:1 to 1 :20, preferably a weight ratio of 10:1 to 1 : 10, preferably a weight ratio of 5:1 to 1 :5. A composition according to this embodiment was surprisingly effective against surprisingly effective against phytopathogenic fungi, such as Pyricularia oryzae, Zymoseptoria tritici, Puccinia recondita f. sp. tritici, preferably against Zymoseptoria tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises fenpropidin at a weight ratio of 50:1 to 1 :50, preferably a weight ratio of 20:1 to 1 : 30, preferably a weight ration of 15:1 to 1 : 20. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises spiroxamine at a weight ratio of 50:1 to 1 :50, preferably a weight ratio of 30:1 to 1 : 30, preferably a weight ratio of 20:1 to 1 : 20. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises pydiflumetofen at a weight ratio of 150:1 to 1 :50, preferably a weight ratio of 100:1 to 1 : 30, preferably a weight ratio of 90:1 to 1 : 20. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises benzovindiflupyr at a weight ratio of 250:1 to 1 :20, preferably a weight ratio of 200:1 to 1 : 10, preferably a weight ratio of 180:1 to 1 :5. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici and Pyricularia oryzae.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises isoflucypram at a weight ratio of 350:1 to 1 :20, preferably a weight ratio of 300:1 to 1 : 10, preferably a weight ratio of 280:1 to 1 : 5. A composition according to this embodiment was surprisingly effective against Zymoseptoria tritici and Puccinia recondita f. sp. tritici, preferably against phytopathogenic fungi, such as Puccinia recondita f. sp. tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises cyprodinil at a weight ratio of 50:1 to 1 :50, preferably a weight ratio of 40:1 to 1 : 40, preferably a weight ratio of 30:1 to 1 : 30, preferably a weight ratio of 20:1 to 1 :20, preferably a weight ratio of 10:1 to 1 :10. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises azoxystrobin at a weight ratio of 100:1 to 1 :20, preferably a weight ratio of 80:1 to 1 :10. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici, Puccinia recondita f. sp. tritici and Pyricularia oryzae.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises pyraclostrobin at a weight ratio of 100:1 to 1 :20, preferably a weight ratio of 80:1 to 1 : 10. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises metyltetrapole at a weight ratio of 200:1 to 1 :20, preferably a weight ratio of 150:1 to 1 : 10, preferably a weight ratio of 100:1 to 1 :5 . A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises proquinazid at a weight ratio of 150:1 to 1 :20, preferably a weight ratio of 100:1 to 1 : 10. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises florylpicoxamid at a weight ratio of 150:1 to 1 :20, preferably a weight ratio of 100:1 to 1 : 10. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Puccinia recondita f. sp. tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises fenpicoxamid at a weight ratio of 150:1 to 1 :20, preferably a weight ratio of 100:1 to 1 : 10. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises folpet at a weight ratio of 50:1 to 1 :50, preferably a weight ratio of 40:1 to 1 : 40, preferably a weight ratio of 30:1 to 1 : 30, preferably a weight ratio of 20:1 to 1 :20, preferably a weight ratio of 10:1 to 1 :10. A composition according to this embodiment was surprisingly effective against phytopathogenic fungi, such as Zymoseptoria tritici and Puccinia recondita f. sp. tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises chlorothalonil at a weight ratio of 50:1 to 1 :100, preferably a weight ratio of 20:1 to 1 :50, preferably a weight ratio of 10:1 to 1 :30. A composition according to this embodiment was surprisingly effective against Zymoseptoria tritici and Puccinia recondita f. sp. tritici. In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises acibenzolar-S-methyl at a weight ratio of 150:1 to 1 :30, preferably a weight ratio of 100:1 to 1 :20, preferably a weight ratio of 80:1 to 1 :10, preferably a weight ratio of 70:1 to 1 :5. Preferably, when component (B) comprises acibenzolar-S-methyl the weight ratio of component (A) to component (B) is from 40:1 to 1 :1 , preferably from 30:1 to 1.5:1.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, may be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises feneptamidoquin at a weight ratio of 20: to 1 :20, preferably a weight ratio of 15: 1 to 1 to 15, preferably a weight ration of 1 : to 1 :10. A composition according to this embodiment was surprisingly effective against Pyricularia oryzae.

A composition according to this embodiment was surprisingly effective against Zymoseptoria tritici and Puccinia recondita f. sp. tritici, and Pyricularia oryzae.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, is a composition wherein component (A) comprises a Streptomyces chrestomyceticusas disclosed herein and wherein component (B) comprises cyclobutrifluram, for instance at a weight ratio of 10,000:1 to 100:1 , such as 8,000:1 to 200:1 , such as 5,000:1 to 500:1. A composition according to this embodiment was found surprisingly effective against phytopathogenic fungi, such as against and Fusarium graminearum.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, is a composition wherein component (A) comprises a Streptomyces chrestomyceticus and wherein component (B) comprises metalaxyl, ethaboxam, oxathiapiprolin, picarbutrazox, and / or fluoxapiprolin. A composition according to this embodiment comprising metalaxyl, ethaboxam, oxathiapiprolin, picarbutrazox, and / or fluoxapiprolin was found surprisingly effective against phytopathogenic fungi, such as Pythium ultimum.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, is a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises metalaxyl, metalaxyl-M, acibenzolar-s-methyl, triticonazole penthiopyrad, sedaxane, thiabendazole, difenoconazole, imidacloprid, thiabendazole, difenoconazole, thiamethoxam, azoxystrobin, pydiflumetofen, fludioxonil, pyraclostrobin, fluoxastrobin, silthiofam, chlorantraniliprole, ethaboxam, ipconazole, prothioconazole, cyantraniliprol, ipconazole, prothioconazole, fluxapyroxad, oxathiapiprolin, fluopyram, benzovindiflupyr, picarbutrazox, mefentrifluconazole, metyltetraprole, cyclobutrifluram, isocycloseram, fluoxapiprolin, ipflufenoquin, and / or inpyrfluxam. A composition according to this embodiment was found surprisingly effective against phytopathogenic fungi, such against Fusarium culmorum.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, is a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises metalaxyl, metalaxyl-M, acibenzolar-s-methyl, triticonazole penthiopyrad, sedaxane, thiabendazole, difenoconazole, imidacloprid, thiabendazole, difenoconazole, , thiamethoxam, azoxystrobin, pydiflumetofen, fludioxonil, pyraclostrobin, fluoxastrobin, silthiofam, chlorantraniliprole, ethaboxam, ipconazole, prothioconazole, cyantraniliprole, ipconazole, prothioconazole, fluxapyroxad, oxathiapiprolin, fluopyram, benzovindiflupyr, picarbutrazox, mefentrifluconazole, metyltetraprole, cyclobutrifluram, isocycloseram, fluoxapiprolin, ipflufenoquin, and / or inpyrfluxam. A composition according to this embodiment was found surprisingly effective against phytopathogenic fungi, such as against Monographella nivalis syn. Microdochium nivale, Fusarium nivale.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, is a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises metalaxyl, metalaxyl-M, acibenzolar-s-methyl, triticonazole, penthiopyrad, sedaxane, thiabendazole, difenoconazole, imidacloprid, thiabendazole, difenoconazole, thiamethoxam, azoxystrobin, pydiflumetofen, fludioxonil, pyraclostrobin, fluoxastrobin, silthiofam, chlorantraniliprole, ethaboxam, ipconazole, prothioconazole, cyantraniliprole, ipconazole, prothioconazole, fluxapyroxad, oxathiapiprolin, fluopyram, benzovindiflupyr, picarbutrazox, mefentrifluconazole, metyltetraprole, cyclobutrifluram, isocycloseram, fluoxapiprolin, ipflufenoquin, and / or inpyrfluxam. A composition according to this embodiment was found surprisingly effective against phytopathogenic fungi, such as against Zymoseptoria (syn. Septoria) tritici.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, is a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises metalaxyl, metalaxyl-M, acibenzolar-s-methyl, triticonazole, penthiopyrad, sedaxane, thiabendazole, difenoconazole, imidacloprid, thiabendazole, difenoconazole, thiamethoxam, azoxystrobin, pydiflumetofen, fludioxonil, pyraclostrobin, fluoxastrobin, silthiofam, chlorantraniliprole, ethaboxam, ipconazole, prothioconazole, cyantraniliprole, ipconazole, fluxapyroxad, oxathiapiprolin, fluopyram, benzovindiflupyr, picarbutrazox, mefentrifluconazole, metyltetraprole, cyclobutrifluram, isocycloseram, fluoxapiprolin, ipflufenoquin, and / or inpyrfluxam. A composition according to this embodiment was found surprisingly effective against phytopathogenic fungi, such as against Glomerella lagenarium syn. Colletotrichum lagenarium.

In one embodiment, a composition comprising components (A) and (B) as active ingredients, is a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein above, and wherein component (B) comprises metalaxyl, metalaxyl-M, acibenzolar-s-methyl, triticonazole, penthiopyrad, sedaxane, thiabendazole, difenoconazole, imidacloprid, thiabendazole, difenoconazole, thiamethoxam, azoxystrobin, pydiflumetofen, fludioxonil, pyraclostrobin, fluoxastrobin, silthiofam, chlorantraniliprole, ethaboxam, ipconazole, prothioconazole, cyantraniliprole, ipconazole, fluxapyroxad, oxathiapiprolin, fluopyram, benzovindiflupyr, picarbutrazox, mefentrifluconazole, metyltetraprole, cyclobutrifluram, isocycloseram, fluoxapiprolin, ipflufenoquin, inpyrfluxam. A composition according to this embodiment was found surprisingly effective against phytopathogenic fungi, such as against Botrytis cinerea.

A composition according to the present invention is an agriculturally acceptable composition.

In one embodiment, the composition according to the present invention further comprises an agriculturally acceptable carrier and, optionally, a surfactant and/or formulation adjuvants. Suitable agricultural adjuvants and/or carriers can be solid or liquid and are substances useful in formulation technology, e.g. natural or regenerated mineral substances, solvents, dispersants, wetting agents, tackifiers, thickeners, binders or fertilizers.

Suitable solvents and liquid carriers include, for example water, organic solvents, oils of vegetable or animal origin, cyclic and aromatic hydrocarbons, alcohols, esters, fatty acids, a glycol or any other suitable liquid carrier known in the art. The solvent or liquid carrier may be water or DMSO.

Suitable solid carriers include, for example ammonium salts, talc, titanium dioxide, pyrophyllite clay, silica, attapulgite clay, kieselguhr, chalk, diatomaxeous earth, lime, calcium carbonate, bentonite clay, fuller’s earth, cotton seed hulls, wheat flour, soybean flour, pumice, wood flour, walnut shell flour and lignin. Water is generally the carrier of choice for the dilution of concentrates. The amount of carrier may typically range from 0.9% to 99.99% by weight of the composition.

A broad range of surface-active agents are advantageously employed in both said liquid and solid compositions, especially those designed to be diluted with carrier before application. These agents, when used, normally comprise from 0.1% to 15% by weight of the formulation. They can be anionic, cationic, non-ionic or polymeric in character and can be employed as emulsifying agents, wetting agents, suspending agents or for other purposes.

Other adjuvants commonly utilized in agricultural compositions include crystallisation inhibitors, viscosity modifiers, suspending agents, spray droplet modifiers, pigments, antioxidants, foaming agents, anti-foaming agents, light-blocking agents, compatibilizing agents, sequestering agents, neutralising agents and buffers, corrosion inhibitors, dyes, odorants, spreading agents, penetration aids, micronutrients, emollients, lubricants and sticking agents.

The composition according to the present invention may be used in unmodified form or, preferably, together with the adjuvants conventionally employed in the art of formulation. To this end they may be conveniently formulated in known manner to emulsifiable concentrates, coatable pastes, directly sprayable or dilutable solutions or suspensions, dilute emulsions, wettable powders, soluble powders, dusts, granulates, and also encapsulations e.g. in polymeric substances. As with the type of the compositions, the methods of application, such as spraying, atomising, dusting, scattering, coating or pouring, are chosen in accordance with the intended objectives and the prevailing circumstances. The compositions may also contain further adjuvants such as stabilizers, antifoams, viscosity regulators, binders or tackifiers as well as fertilizers, micronutrient donors or other formulations for obtaining special effects.

Component (A) as disclosed herein may be a formulation, wherein Streptomyces chrestomyceticus as disclosed herein is formulated as an oil dispersion (OD), a non-aqueous dispersion (NAD) or a flowable formulation.

An oil dispersion (OD) is a solid active ingredient dispersed in oil, which is a water immiscible carrier. An oil dispersion typically comprises (in wt%) active ingredient (5-60%) non-aqueous dispersants (1-10% of solids) aqueous dispersants (1-10% of solids) emulsifier (2-10% of iol) rheology modifier (0.2- 5%), and an oil carrier to make up 100%. Suitable oils in an oil dispersion can be mineral oils, paraffinic oils, vegetable oils or methylated oils.

Suitable adjuvants, dispersants, emulsifiers and rheology modifiers in an oil dispersion depend on the type of oil and are known in the art. Suitable emulsifiers can be alcohol ethoxylates/alcoxylates, such as C16/18 ethoxylates, or C16/C18 alcoxylate, or block co-polymers. An adjuvant can be an alkyl polyglucoside. Suitable rheology modifiers can be clay, hydrogenated castor oil and derivatives, fumed silicam polyamides, polyesters or agrilan ODS.

A non-aqueous dispersion (NAD) is a liquid formulation wherein a solid ingredient is dispersed in a water-miscible carrier. The solid ingredient is uniformly suspended in the carrier but not dissolved.

Suspension concentrates are aqueous formulations in which finely divided solid particles of the active ingredient are suspended. Such formulations include anti-settling agents and dispersing agents and may further include a wetting agent to enhance pesticidal activity as well an anti-foam and a crystal growth inhibitor. In use, these concentrates are diluted in water and normally applied as a spray to the area to be treated. The amount of active ingredient may range from 0.5% to 95% of the concentrate.

Wettable powders are in the form of finely divided particles which disperse readily in water or other liquid carriers. The particles contain an active ingredient retained in a solid matrix. Typical solid matrices include fuller’s earth, kaolin clays, silicas and other readily wet organic or inorganic solids. Wettable powders normally contain from 5% to 95% of an active ingredient plus a small amount of wetting, dispersing or emulsifying agent.

Emulsifiable concentrates are homogeneous liquid compositions dispersible in water or other liquid and may consist entirely of an active ingredient a liquid or solid emulsifying agent, or may also contain a liquid carrier, such as xylene, heavy aromatic naphthas, isophorone and other non-volatile organic solvents. In use, these concentrates are dispersed in water or other liquid and normally applied as a spray to the area to be treated. The amount of active ingredient may range from 0.5% to 95% of the concentrate.

Granular formulations include both extrudates and relatively coarse particles and are usually applied without dilution to the area in which treatment is required. Typical carriers for granular formulations include sand, fuller’s earth, attapulgite clay, bentonite clays, montmorillonite clay, vermiculite, perlite, calcium carbonate, brick, pumice, pyrophyllite, kaolin, dolomite, plaster, wood flour, ground corn cobs, ground peanut hulls, sugars, sodium chloride, sodium sulphate, sodium silicate, sodium borate, magnesia, mica, iron oxide, zinc oxide, titanium oxide, antimony oxide, cryolite, gypsum, diatomaceous earth, calcium sulphate and other organic or inorganic materials which absorb or which can be coated with the active compound.

The compositions of the invention may be employed in any conventional form, for example in the form of a twin pack, a powder for dry seed treatment (DS), an emulsion for seed treatment (ES), a flowable concentrate for seed treatment (FS), a solution for seed treatment (LS), a water dispersible powder for seed treatment (WS), a capsule suspension for seed treatment (CF), a gel for seed treatment (GF), an emulsion concentrate (EC), a suspension concentrate (SC), a suspo-emulsion (SE), a capsule suspension (CS), a water dispersible granule (WG), an emulsifiable granule (EG), an emulsion, water in oil (EG), an emulsion, oil in water (EW), a micro-emulsion (ME), an oil dispersion (OD), an oil miscible flowable (OF), an oil miscible liquid (OL), a soluble concentrate (SL), an ultra-low volume suspension (SU), an ultra-low volume liquid (UL), a technical concentrate (TK), a dispersible concentrate (DC), a wettable powder (WP) or any technically feasible formulation in combination with agriculturally acceptable adjuvants.

Such compositions may be produced in conventional manner, e.g. by mixing the active ingredients with appropriate formulation inerts (diluents, solvents, fillers and optionally other formulating ingredients such as surfactants, biocides, anti-freeze, stickers, thickeners and compounds that provide adjuvancy effects). Also conventional slow release formulations may be employed where long lasting efficacy is intended. Particularly formulations to be applied in spraying forms, such as water dispersible concentrates (e.g. EC, SC, DC, OD, SE, EW, EO and the like), wettable powders and granules, may contain surfactants such as wetting and dispersing agents and other compounds that provide adjuvancy effects, e.g. the ondensation product of formaldehyde with naphthalene sulphonate, an alkylarylsulphonate, a lignin sulphonate, a fatty alkyl sulphate, and ethoxylated alkylphenol and an ethoxylated fatty alcohol.

A seed dressing formulation is applied in a manner known per se to the seeds employing the combination of the invention and a diluent in suitable seed dressing formulation form, e.g. as an aqueous suspension or in a dry powder form having good adherence to the seeds. Such seed dressing formulations are known in the art. Seed dressing formulations may contain the single active ingredients or the combination of active ingredients in encapsulated form, e.g. as slow release capsules or microcapsules.

In general, the formulations include from 0.01 to 95% by weight of active ingredients, from 0 to 20% agriculturally acceptable surfactant and 10 to 99.99% solid or liquid formulation inerts and adjuvant(s), the active ingredients consisting of at least the components (A) and (B) as defined herein, and optionally other active agents, particularly microbiocides or conservatives or the like. Concentrated forms of compositions generally contain in between about 2 and 80%, preferably between about 5 and 70% by weight of active ingredients. Application forms of formulation may for example contain from 0.01 to 20% by weight, preferably from 0.01 to 5% by weight of active ingredients. Whereas commercial products will preferably be formulated as concentrates, the end user will normally employ diluted formulations.

In one aspect the present invention relates to a method of controlling or preventing phytopathogenic diseases, preferably phytopathogenic fungi, on a plant or on plant propagation material and / or on harvested food crops, which comprises applying to the plant, on plant propagation material, the locus thereof, and / or on harvested food crops the composition according to the present invention.

Controlling or preventing means reducing infestation by phytopathogenic or spoilage microorganisms, especially fungal organisms, to such a level that an improvement is demonstrated.

A preferred method of controlling or preventing an infestation of crop plants by phytopathogenic microorganisms, especially fungal organisms, comprises the application of a composition according to the present invention is foliar application. The frequency of application and the rate of application will depend on the risk of infestation by the corresponding pathogen. However, the compounds A and/or B of the composition according to the present invention can also penetrate the plant through the roots via the soil (systemic action) by drenching the locus of the plant with a liquid formulation, or by applying said compounds in solid form to the soil, e.g. in granular form (soil application). In crops of water rice such granulates can be applied to the flooded rice field.

Components (A) and (B) of the composition according to the present invention may be applied simultaneously, such as in a ready to mix formulation, or in a sequential manner. The method according to the present invention comprises applying the composition according to the present invention, wherein the components (A) and (B) are applied in simultaneous or a sequential manner on the plant, the plant propagation material or the locus thereof.

Any phytopathogenic disease can be controlled or prevented in the method according to the present invention. The composition of the invention may be used to control plant diseases caused by a broad spectrum of plant pathogens in the Basidiomycete, Ascomycete, Oomycete and/or Deuteromycete, Blasocladiomycete, Chrytidiomycete, Glomeromycete, Mucoromycete classes, and/or Oomycete classes. These pathogens may include:

Oomycetes, including Phytophthora diseases such as those caused by Phytophthora capsici, Phytophthora infestans, Phytophthora sojae, Phytophthora fragariae, Phytophthora nicotianae, Phytophthora cinnamomi, Phytophthora citricola, Phytophthora citrophthora and Phytophthora erythroseptica; Pythium diseases such as those caused by Pythium aphanidermatum, Pythium arrhenomanes, Pythium graminicola, Pythium irregulare, Pythium sylvaticum and Pythium ultimum; diseases caused by Peronosporales such as Peronospora destructor, Peronospora parasitica, Plasmopara viticola, Plasmopara halstedii, Pseudoperonospora cubensis, Albugo Candida, Sclerophthora macrospora and Bremia lactucae' and others such as Aphanomyces spp., Labyrinthula zosterae, Peronosclerospora sorghi and Sclerospora graminicola'

Ascomycetes, including blotch, spot, blast or blight diseases and/or rots for example those caused by Pleosporales such as Stemphylium solani, Stagonospora tainanensis, Spilocaea oleaginea, Setosphaeria turcica, Pyrenochaeta lycoperisici, Pleospora herbarum, Phoma destructiva, Phaeosphaeria herpotrichoides, Phaeocryptocus gaeumannii, Ophiosphaerella graminicola, Ophiobolus graminis, Leptosphaeria maculans, Hendersonia creberrima, Helminthosporium triticirepentis, Setosphaeria turcica, Drechslera glycines, Didymella bryoniae, Cycloconium oleagineum, Corynespora cassiicola, Cochliobolus sativus, Bipolaris cactivora, Venturia inaequalis, Pyrenophora teres, Pyrenophora tritici-repentis, Alternaria alternata, Alternaria brassicicola, Alternaria solani and Alternaria tomatophila, Capnodiales such as Septoria tritici, Septoria nodorum, Septoria glycines, Cercospora arachidicola, Cercospora sojina, Cercospora zeae-maydis, Cercosporella capsellae and Cercosporella herpotrichoides, Cladosporium carpophilum, Cladosporium effusum, Passalora fulva, Cladosporium oxysporum, Dothistroma septosporum, Isariopsis clavispora, Mycosphaerella fijiensis, Mycosphaerella graminicola, Mycovellosiella koepkeii, Phaeoisariopsis bataticola, Pseudocercospora vitis, Pseudocercosporella herpotrichoides, Ramularia beticola, Ramularia collo-cygni, Magnaporthales such as Gaeumannomyces graminis, Magnaporthe grisea, Pyricularia oryzae, Diaporthales such as Anisogramma anomala, Apiognomonia errabunda, Cytospora platani, Diaporthe phaseolorum, Discula destructiva, Gnomonia fructicola, Greeneria uvicola, Melanconium juglandinum, Phomopsis viticola, Sirococcus clavigignenti-juglandacearum, Tubakia dryina, Dicarpella spp. , Valsa ceratosperma, and others such as Actinothyrium graminis, Ascochyta pisi, Aspergillus flavus, Aspergillus fumigatus, Aspergillus nidulans, Asperisporium caricae, Blumeriella jaapii, Candida spp., Capnodium ramosum, Cephaloascus spp., Cephalosporium gramineum, Ceratocystis paradoxa, Chaetomium spp., Hymenoscyphus pseudoalbidus, Coccidioides spp., Cylindrosporium padi, Diplocarpon malae, Drepanopeziza campestris, Elsinoe ampelina, Epicoccum nigrum, Epidermophyton spp., Eutypa lata, Geotrichum candidum, Gibellina cerealis, Gloeocercospora sorghi, Gloeodes pomigena, Gloeosporium perennans; Gloeotinia temulenta, Griphospaeria corticola, Kabatiella lini, Leptographium microsporum, Leptosphaerulinia crassiasca, Lophodermium seditiosum, Marssonina graminicola, Microdochium nivale, Monilinia fructicola, Monographella albescens, Monosporascus cannonballus, Naemacyclus spp., Ophiostoma novo-ulmi, Paracoccidioides brasiliensis, Penicillium expansum, Pestalotia rhododendri, Petriellidium spp., Pezicula spp., Phialophora gregata, Phyllachora pomigena, Phymatotrichum omnivora, Physalospora abdita, Plectosporium tabacinum, Polyscytalum pustulans, Pseudopeziza medicaginis, Pyrenopeziza brassicae, Ramulispora sorghi, Rhabdocline pseudotsugae, Rhynchosporium secalis, Sacrocladium oryzae, Scedosporium spp., Schizothyrium pomi, Sclerotinia sclerotiorum , Sclerotinia minor, Sclerotium spp., Typhula ishikariensis, Seimatosporium mariae, Lepteutypa cupressi, Septocyta ruborum, Sphaceloma perseae, Sporonema phacidioides, Stigmina palmivora, Tapesia yallundae, Taphrina bullata, Thielviopsis basicola, Trichoseptoria fructigena, Zygophiala jamaicensis; powdery mildew diseases for example those caused by Erysiphales such as Blumeria graminis, Erysiphe polygon!, Uncinula necator, Sphaerotheca fuligena, Podosphaera leucotricha, Podospaera macularis Golovinomyces cichoracearum , Leveillula taurica, Microsphaera diffusa, Oidiopsis gossypii, Phyllactinia guttata and Oidium arachidis; molds for example those caused by Botryosphaeriales such as Dothiorella aromatica, Diplodia seriata, Guignardia bidwellii, Botrytis cinerea, Botryotinia allii, Botryotinia fabae, Fusicoccum amygdali, Lasiodiplodia theobromae, Macrophoma theicola, Macrophomina phaseolina, Phyllosticta cucurbitacearum; anthracnoses for example those caused by Glommerelales such as Colletotrichum gloeosporioides, Colletotrichum lagenarium, Colletotrichum gossypii, Glomerella cingulata, and Colletotrichum graminicola; and wilts or blights for example those caused by Hypocreales such as Acremonium strictum, Claviceps purpurea, Fusarium culmorum, Fusarium graminearum, Fusarium virguliforme, Fusarium oxysporum, Fusarium subglutinans, Fusarium oxysporum f.sp. cubense, Gerlachia nivale, Gibberella fujikuroi, Gibberella zeae, Gliocladium spp., Myrothecium verrucaria, Nectria ramulariae, Trichoderma viride, Trichothecium roseum, and Verticillium theobromae;

Basidiomycetes, including smuts for example those caused by Ustilaginales such as Ustilaginoidea virens, Ustilago nuda, Ustilago tritici, Ustilago zeae, rusts for example those caused by Pucciniales such as Cerotelium fici, Chrysomyxa arctostaphyli, Coleosporium ipomoeae, Hemileia vastatrix, Puccinia arachidis, Puccinia cacabata, Puccinia graminis, Puccinia recondita, Puccinia sorghi, Puccinia hordei, Puccinia striiformis f.sp. Hordei, Puccinia striiformis f.sp. Secalis, Pucciniastrum coryli, or Uredinales such as Cronartium ribicola, Gymnosporangium juniperi-viginianae, Melampsora medusae, Phakopsora pachyrhizi, Phragmidium mucronatum, Physopella ampelosidis, Tranzschelia discolor and Uromyces viciae-fabae; and other rots and diseases such as those caused by Cryptococcus spp., Exobasidium vexans, Marasmiellus inoderma, Mycena spp., Sphacelotheca reiliana, Typhula ishikariensis, Urocystis agropyri, Itersonilia perplexans, Corticium invisum, Laetisaria fuciformis, Waitea circinata, Rhizoctonia solani, Thanetephorus cucurmeris, Entyloma dahliae, Entylomella microspora, Neovossia moliniae and Tilletia caries;

Blastocladiomycetes, such as Physoderma maydis;

Mucoromycetes, such as Choanephora cucurbitarum. Mucor spp.; Rhizopus arrhizus; as well as diseases caused by other species and genera closely related to those listed above.

Phytopatogenic diseases that are advantageously controlled or prevented are phytopathogenic fungi belonging to a genus belonging to Zymoseptor/a, Puccinia, Pyricularia, Fusarium, Pythium, Monographella, Microdochium, Glomerella, Colletotrichum, and / or Botrytis, preferably belonging to a species belonging to Zymoseptoria tritici, Puccinia recondita f. sp. tritici (Puccinia triticina), Pyricularia oryzae, Pythium ultimum, Fusarium culmorum, Fusarium graminearum Monographella nivalis syn. Microdochium nivale, Fusarium nivale, Septoria tritici, Glomerella lagenarium syn. Colletotrichum lagenarium (Colletotrichum orbiculare), and/ or Botrytis cinerea.

The composition according to the present invention can be used in the agricultural sector and related fields of use for controlling plant pests or on non-living materials for control of spoilage microorganisms or organisms potentially harmful to man. The composition can be used to inhibit or destroy the pests that occur on plants or parts of plants (fruit, blossoms, leaves, stems, tubers, roots) of different crops of useful plants, while at the same time protecting also those parts of the plants that grow later e.g. from phytopathogenic microorganisms.

In the scope of the present invention, a plant includes a useful plant and/or crop.

The term “locus” as used herein means fields in or on which plants are growing, or where seeds of cultivated plants are sown, or where seed will be placed into the soil. It includes soil, seeds, and seedlings, as well as established vegetation.

The term “plant propagation material” is understood to denote generative parts of the plant, such as seeds, which can be used for the multiplication of the latter, and vegetative material, such as cuttings or tubers, for example potatoes. There may be mentioned for example seeds (in the strict sense), roots, fruits, tubers, bulbs, rhizomes and parts of plants. Germinated plants and young plants which are to be transplanted after germination or after emergence from the soil, may also be mentioned. These young plants may be protected before transplantation by a total or partial treatment by immersion. Preferably “plant propagation material” is understood to denote seeds.

Useful plants and / or target crops in accordance with the invention include conventional as well as genetically enhanced or engineered varieties such as, for example, insect resistant (e.g. Bt. and VIP varieties) as well as disease resistant, herbicide tolerant (e.g. glyphosate- and glufosinate-resistant maize varieties) and nematode tolerant varieties. Useful plants include plants transformed by the use of recombinant DNA techniques, for example, to be capable of synthesising one or more selectively acting toxins, such as are known, for example, from toxin-producing bacteria. Examples of toxins which can be expressed include 8-endotoxins, vegetative insecticidal proteins (Vip), insecticidal proteins of bacteria colonising nematodes, and toxins produced by scorpions, arachnids, wasps and fungi.

Plants, including target crops and/or useful plants to be protected in a method of the invention typically comprise perennial and annual crops, such as berry plants for example blackberries, blueberries, cranberries, raspberries and strawberries; cereals for example barley, maize (corn), millet, oats, rice, rye, sorghum triticale and wheat; fibre plants for example cotton, flax, hemp, jute and sisal; field crops for example sugar and fodder beet, coffee, hops, mustard, oilseed rape (canola), poppy, sugar cane, sunflower, tea and tobacco; fruit trees for example apple, apricot, avocado, banana, cherry, citrus, nectarine, peach, pear and plum; grasses for example Bermuda grass, bluegrass, bentgrass, centipede grass, fescue, ryegrass, St. Augustine grass and Zoysia grass; herbs such as basil, borage, chives, coriander, lavender, lovage, mint, oregano, parsley, rosemary, sage and thyme; legumes for example beans, lentils, peas and soya beans; nuts for example almond, cashew, ground nut, hazelnut, peanut, pecan, pistachio and walnut; palms for example oil palm; ornamentals for example flowers, shrubs and trees; other trees, for example cacao, coconut, olive and rubber; vegetables for example asparagus, aubergine, broccoli, cabbage, carrot, cucumber, garlic, lettuce, marrow, melon, okra, onion, pepper, potato, pumpkin, rhubarb, spinach and tomato; and vines for example grapes.

A plant in a method according to the present invention preferably comprises wheat, barley, rice, corn, soya, sugar beet, banana, tomato, cucumber, and / or groundnut. Preferably the plant comprises wheat and / or rice.

The composition comprising components (A) and (B) as defined herein may also be applied to seeds (coating) by impregnating the seeds or tubers either with a liquid formulation of the composition of the invention or coating them with a solid formulation.

A method of controlling or preventing a phytopathogenic disease includes spraying, atomizing, dusting, brushing on, dressing, scattering or pouring - which are to be selected to suit the intended aims of the prevailing circumstances. Typical rates of concentration are between 0.1 and 1000 ppm, preferably between 0.1 and 500 ppm, of active ingredient. The rate of application per hectare is preferably 1 g to 2000 g of active ingredient per hectare, more preferably 10 to 1000 g/ha, most preferably 10 to 600 g/ha. When used as seed drenching agent, convenient dosages are from 10mg to 1g of active substance per kg of seeds. When the composition of the present invention is used for treating seed, rates of 0.001 to 50 g of the composition per kg of seed, preferably from 0.01 to 10g per kg of seed are generally sufficient.

In one embodiment, the method comprises applying an effective amount of component A comprising Streptomyces as disclosed herein above, wherein the effective amount comprises from 2*10² to 5*10¹⁷ , from 3*10² to 5*10¹⁶, from 5*10² to 5*10¹⁵ , from 2*10² to 5*10¹⁴, from 2*10² to 5*10¹³, preferably 5*10² to 5*10¹², 1*10® to 5*10¹¹ , from 5*10³ to 1*10¹¹ , from 1*10⁴ to 5*10¹⁰, from 5*10⁴ to 1*10¹° , from 1*10⁵ to 5*10⁹, from 5*10⁵ to 1*10⁹ , from 1*10⁶ to 5*10⁸, from 5*10® to 1*10⁸ colony forming unit (cfu) of the Streptomyces such as Streptomyces chrestomyceticus, per hectare.

An effective amount of component A comprising Streptomyces as disclosed herein above, comprises from 1 g to 10 kg / per hectare (ha), such as from 5 g to 5 kg, such as from 10 g to 1 kg / ha, such as from 50 g to 800 g / ha, such as from 100 g to 700 g / ha, such as from 200 to 600 g / ha. The weight in g and kg is dry weight of the Streptomyces.

In one embodiment the method according to the present invention comprises treating plant propagation material wherein the plant propagation material is seed and the effective amount comprises 5x10² to 5x10¹⁵, 2x10³ to 5x10¹⁴, from 5x10³ to 5x10¹³, ^from 2x10⁵to 5x10¹², preferably 5*10² to 5*10¹², 1*10³ to 5*10¹¹ , from 5*10³ to 1 *10¹¹ , from 1 *10⁴ to 5*10¹°, from 5*10⁴ to 1 *10¹° , from 1 *10⁵ to 5*10⁹, from 5*10⁵ to 1*10⁹ , from 1*10® to 5*10®, from 5*10® to 1*10® colony forming unit (cfu) of the Streptomyces in component A, such as Streptomyces chrestomyceticus as disclosed herein per kg of seed.

When the plant propagation material is seed, an effective amount of component (A) comprising Streptomyces chrestomyceticus as disclosed herein above, may also comprise from 0.001 g to 100 g / per kg of seeds, such as from 0.005 g to 80 g / kg seeds, such as from 0.01 g to 50 g / kg seeds, such as from 0.5 g to 10 g / kg seeds, The weight in g dry weight of component A comprising Streptomyces per kg dry weight of seeds.

Suitably, the composition according to the present invention can be applied either preventative, meaning prior to disease development or curative, meaning after disease development.

In one further aspect the present invention relates to a plant, plant propagation material, the locus thereof and/or a harvested food crop comprising a composition as disclosed herein. The description related to the features disclosed herein above are also applicable to this aspect of the invention.

A composition comprising components (A) and (B) as active ingredients, can be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein, such as a Streptomyces sp. Saigon413, deposited at the Westerdijk institute under accession number CBS149411 , and wherein component (B) comprises one or more of the following components:

(4E,10Z)-tetradeca-4, 10-dienyl acetate, (7E,9Z)-dodeca-7,9-dien-1-yl acetate, (E)-6-methylhept-2- en-4-ol, (E)-dec-5-en-1 -yl acetate with (E)-dec-5-en-1-ol, (E)-tridec-4-en-1 -yl acetate, (S)-bioallethrin, (Z)- dodec-7-en-1-yl acetate, (Z)-hexadec-l 1-en-1-yl acetate, (Z)-hexadec-11-enal, (Z)-hexadec-l 3-en-11-yn-

1-yl acetate, (Z)-icos-13-en-10-one, (Z)-tetradec-7-en-1-al, (Z)-tetradec-9-en-1-ol, (Z)-tetradec-9-en-1-yl acetate, 1 ,1-bis(4-chlorophenyl)-2-ethoxyethanol, 1-(2-chlorophenyl)-3,3-dimethyl-2-(1 ,2,4-triazol-1 - ylmethyl)butan-2-ol, 1-(5-bromo-2-pyridyl)-2-(2,4-difluorophenyl)-1 , 1 -difluoro-3-(1 ,2,4-triazol-1-yl)propan-

2-ol, 1 -hydroxy-1 H-pyridine-2-thione, 1 -methylcyclopropene, 1 -naphthaleneacetamide, 1 -naphthylacetic acid, 2,2-dichlorovinyl 2-ethylsulfinylethyl methyl phosphate, 2,4-D, 2,4-DB, 2,6-dichloro-N-(4- trifluormethylbenzyl)benzamide, 2-(1 ,3-dithiolan-2-yl)phenyl dimethylcarbamate, 2-(2-butoxyethoxy)ethyl piperonylate, 2-(4,5-dimethyl-1 ,3-dioxolan-2-yl)phenyl methylcarbamate, 2-(difluoromethyl)-N-((3R)-1 ,1 ,3- trimethylindan-4-yl) pyridine-3-carboxamide, 2-(octylthio)ethanol, 2-bromo-2-bromomethyl-pentanedinitrile, 2-chlorovinyl diethyl phosphate, 2-imidazolidone, 2-methyl(prop-2-ynyl)aminophenyl methylcarbamate, 2- thiocyanatoethyl laurate, 3-(4-chlorophenyl)-5-methylrhodanine, 3-(difluoromethyl)-1-methyl-N-[1 ,1 ,3- trimethylindan-4-yl]pyrazole-4-carboxamide, 3-(difluoromethyl)-N-(7-fluoro-1 ,1 ,3,3-tetramethyl-indan-4-yl)- 1-methyl-pyrazole-4-carboxamide, 3-(difluoromethyl)-N-(7-fluoro-1 ,1 ,3,3-tetramethyl-indan-4-yl)-1-methyl- pyrazole-4-carboxamide, 3-chloro-6-methyl-5-phenyl-4-(2,4,6-trifluorophenyl)pyridazine, 3-methyl-1- phenylpyrazol-5-yl dimethylcarbamate, 3-phenylphenol, 4,5-dichlorodithiol-3-one, 4-(2,6-difluorophenyl)-6- methyl-5-phenyl-pyridazine-3-carbonitrile, 4-(2-bromo-4-fluoro-phenyl)-N-(2-chloro-6-fluoro-phenyl)-2,5- dimethyl-pyrazol-3-amine, 4-(quinoxalin-2-ylamino)benzenesulfonamide, 4-[[6-[2-(2,4-difluorophenyl)-1 ,1- difluoro-2-hydroxy-3-(1 ,2,4-triazol-1-yl)propyl]-3-pyridyl]oxy]benzonitrile, 4-chloro-2-(2-chloro-2-methyl- propyl)-5-[(6-iodo-3-pyridyl)methoxy]pyridazin-3-one, 4-CPA, 4-methyl(prop-2-ynyl)amino-3,5-xylyl methylcarbamate, 4-methylnonan-5-ol with 4-methylnonan-5-one, 4-phenylphenol, 5-(1 ,3-benzodioxol-5- yl)-3-hexylcyclohex-2-enone, 5-amino-1 ,3,4-thiadiazole-2-thiol, 5-fluoro-2-(p-tolylmethoxy)pyrimidin-4- amine, 5-hydroxy-6-methyl-4-(((E)-pyridin-3-ylmethylene)amino)-4,5-dihydro-1 ,2,4-triazin-3(2H)-one, 5- methyl-6-thioxo-1 ,3,5-thiadiazinan-3-ylacetic acid, 8-hydroxyquinoline sulfate, 11-ethyl-10,12-dioxo-2,5,8- trithia-4, 11 -diazatricyclo[7.3.0.03,7]dodeca-1 (9),3,6-triene-6-carbonitrile, 14-methyloctadec-1 -ene, [(9Z,11 E)-tetradeca-9, 11 -dienyl] acetate, [(Z)-dodec-9-enyl] acetate, abamectin, acephate, acequinocyl, acetamiprid, acethion, acetoprole, acibenzolar, acibenzolar-S-methyl, acrinathrin, Adoxophyes orana GV, Agrobacterium radiobacter, alanycarb, albendazole, aldicarb, allethrin, allosamidin, allyl alcohol, allyxycarb, alpha-ecdysone, alpha-multistriatin, Amblyseius spp., ametoctradin, amidithion, amidoflumet, amidothioate, aminocarb, amisulbrom, amiton, amiton hydrogen oxalate, amitraz, anabasine, Anagrapha falcifera NPV, Anagrus atomus, ancymidol, anilazine, anisiflupurin, anthraquinone, antu, Aphelinus abdominalis, Aphidius colemani, Aphidoletes aphidimyza, athidathion, aureofungin, Autographa californica NPV, avermectin B1 a, azaconazole, azadirachtin A, azafenidin, azamethiphos, azinphos-ethyl, azinphos- methyl, azithiram, azoxystrobin, Bacillus sphaericus (Neide), Bacillus thuringiensis, Bacillus thuringiensis delta endotoxin, Bacillus thuringiensis ssp. aizawai, baculovirus, barthrin, Beauveria brongniartii, benalaxyl, benalaxyl-M, benazepril, benclothiaz, benfuracarb, benomyl, bensultap, benthiavalicarb, benzalkonium chloride, benzamorf, benzothiostrobin, benzovindiflupyr, beta-cyfluthrin, beta-cy permethrin, bethoxazin, bifemetstrobin, bifenazate, bifenthrin, binapacryl, bioallethrin, bioethanomethrin, bio permethrin, bioresmethrin, bisthiosemi, bistrifluron, bitertanol, bixafen, blasticidin-S, borax, bordeaux mixture, boscalid, brodifacoum, brofenvalerate, brofluthrinate, bromadiolone, bromfenvinfos, bromophos, bromophos-ethyl, bromuconazole, bufencarb, bupirimate, buprofezin, buserelin, busulfan, but-3-ynyl N-[6-[[(Z)-[(1- methyltetrazol-5-yl)-phenyl-methylene]amino]oxymethyl]-2-pyridyl]carbamate, butacarb, butathiofos, butocarboxim, butonate, butopyronoxyl, butoxy(polypropylene glycol), butoxycarboxim, butylamine, cadusafos, calciferol, calcium phosphate, calcium polysulfide, cambendazole, captafol, captan, carbanolate, carbaryl, carbendazim, carbendazim hydrochloride, carbofuran, carbosulfan, carboxin, carprofen, carpropamid, cartap, cartap hydrochloride, cefalexin, cefovecin, cefquinome, ceftiour, cestex, cevadine, chinomethionat, chitosan, chlobenthiazone, chloralose, chlorantraniliprole, chlorbenside, chlordimeform, chlorethephon, chlorethoxyfos, chlorfenapyr, chlorfenazole, chlorfentazine, chlorfenvinphos, chlorfluazuron, chlormephos, chlormequat, chlorodimeform hydrochloride, chloroinconazide, chloromebuform, chloromethiuron, chloroneb, chlorothalonil, chlorphoxim, chlorprazophos, chlorpyrifos, chlorpyrifos-methyl, chlortetracycline, chlorthiophos, chlozolinate, cholecalciferol, chromafenozide, Chrysoperla carnea, cinerin, cinerin I, cinerin II, cis-jasmone, cis- resmethrin, cismethrin, clenbuterol, climbazole, cloethocarb, clofencet, clorsulon, clothianidin, clozylacon (acetamide), codlelure, copper acetate, copper hydroxide, copper naphthenate, copper octanoate, copper oleate, copper oxide, copper oxychloride, copper silicate, copper sulfate, copper(ll) carbonate, coumachlor, coumafene, coumafuryl, Coumatetralyl, coumethoxystrobin (jiaxiangjunzhi), coumithoate, coumoxystrobin, cryolite, Cryptolaemus montrouzieri, cuelure, cufraneb, cuprous(l) oxide, cyanofenphos, cyanthoate, cyazofamid, cybutryne, cyclafuramid, cyclethrin, cyclobutrifluram, Cydia pomonella GV, cyenopyrafen, cyflufenamid, cyflumetofen, cyfluthrin, cyhalothrin, cymiazole, cymoxanil, cypermethrin (alphametrin), cyphenothrin, cyproconazole, cyprodinil, cyprodinil, cyromazine, cytokinins, D-tetramethrin, Dacnusa sibirica, DAEP, dazomet, DCPM, debacarb, decarbofuran, deltamethrin, demephion-O, demephion-S, demeton-O, demeton-S, demeton-S-methyl, demeton-S-methylsulphon, diafenthiuron, dialifos, diazinon, dibutyl adipate, dibutyl phthalate, dibutyl succinate, dicapthon, dichlobentiazox, dichlofluanid, dichlone, dichlorprop, dichlorvos, dichlozoline, diclocymet, diclomezine, dicloran, dicofol, dicresyl, dicrotophos, dicyclanil, dicyclopentadiene, diethofencarb, diethyltoluamide, difenacoum, difenoconazole, difenzoquat, difethialone, diflovidazin, diflubenzuron, diflumetorim, Diglyphus isaea, dimatif, dimefluthrin, dimetan, dimethachlon, dimethipin, dimethirimol, dimethoate, dimethomorph, dimethrin, dimethyl disulfide, dimethyl phthalate, dimetilan, dimoxystrobin, dinactin, diniconazole, diniconazole-M, dinobuton, dinocap, dinocton, dinoseb, dinotefuran, diofenolan, dioxabenzofos, diphenylamine, dipyrithione, disparlure, disulfiram, disulfoton, ditalimfos, dithianon, dithicrofos, dithiocarbamate, dodec-8-en-1-yl acetate, dodemorph, dodicin, dodine, dofenapyn, dominicalure, doramectin, drazoxolon, DSP, ecdysterone, edifenphos, emamectin benzoate, EMPC, empenthrin, Encarsia formosa, endosulfan, endothal, endothion, enestroburin (enoxastrobin), enrofloxacin, entomopathogenic bacteria, entomopathogenic fungi, entomopathogenic virus, EPBP, epoxiconazole, eprinomectin, Eretmocerus eremicus, esfenvalerate, etaconazole, ethaboxam, ethiofencarb, ethion, ethiprole, ethirimol, ethoate-methyl, ethoprophos, ethoxyquin, ethyl 4- methyloctanoate, ethyl formate, ethyl hexanediol, etofenprox, etoxazole, etridiazole, etrimfos, eugenol, eurax, EXD, exo-brevicomin, famoxadone, famphur, farnesol with nerolidol, febantel, fenamidone, fenaminstrobin, fenamiphos, fenarimol, fenazaquin, fenbendazole, fenbuconazole, fenbutatin oxide, feneptamidoquin, fenethacarb, fenfluthrin, fenfuram, fenhexamid, fenitrothion, fenobucarb, fenopyramid, fenothiocarb, fenoxacrim, fenoxanil, fenoxycarb, fenpiclonil, fenpicoxamid, fenpirithrin, fenpropathrin, fenpropidin, fenpropimorph, fenpyrazamine, fenpyroximate, fensulfothion, fenthion, fenthion-ethyl, fentin, fentin acetate, fentin chloride, fentin hydroxide, fenvalerate, ferbam, ferimzone, ferric phosphate, fipronil, flocoumafen, flonicamid, florfenicol, florylpicoxamid, fluacrypyrim, fluazinam, fluazuron, flubendazole, flubendiamide, flubeneteram, flubenzimin, flucycloxuron, flucycloxuron, flucythrinate, fludioxonil, fluenetil, flufenerim, flufenoxuron, flufenoxystrobin, flufenprox, fluindapyr, flumetralin, flumetylsulforim, flumorph, fluopicolide, fluopimomide, fluopyram, fluoroimide, fluoxapiprolin, fluoxastrobin, fluoxytioconazole, flupyrazofos, fluquinconazole, flusilazole, flusulfamide, flutianil, flutolanil, flutriafol, fluxapyroxad, folpet, fonofos, forchlorfenuron, formaldehyde, formetanate, formetanate hydrochloride, formothion, formparanate, fosetyl, fosetyl-aluminium, fosmethilan, fosthiazate, fosthietan, frontalin, fuberidazole, furalaxyl, furametpyr, furathiocarb, furethrin, furfural, gibberellic acid, glyodin, glyphosate, grandlure I, grandlure II, grandlure III, grandlure IV, guazatine triacetate, halfenprox, halofenozide, hemel, heptenophos, Heterorhabditis bacteriophora and H. megidis, hexaconazole, hexadecyl cyclopropanecarboxylate, hexaflumuron, hexalure, hexamide, hexythiazox, Hippodamia convergens, huanjunzuo (rac-(1 S,2S)-1-(4-chlorophenyl)-2-(1 ,2,4-triazol-1-yl)cycloheptanol), hydramethylnon, hydrated lime (calcium hydroxide), hymexazol, hyquincarb, icaridin, imanin (hypericin), imazalil, imazalil sulfate, imibenconazole, imidacloprid, iminoctadine, indoxacarb, inpyrfluxam, iodocarb, ipconazole, ipfentrifluconazole, ipflufenoquin, iprobenfos (IBP), iprodione, iprovalicarb, ipsdienol, ipsenol, IPSP, isamidofos, isazofos, isocarbophos, isofetamid, isoflucypram, isolan, isoprocarb, isoprothiolane, isopyrazam, isothioate, isotianil, isoxathion, ivermectin, japonilure, jasmolin I, jasmolin II, juvenile hormone I, juvenile hormone II, juvenile hormone III, kadethrin, kanamycin, kasugamycin, kasugamycin hydrochloride hydrate, kinetin, kinoprene, kresoxim-methyl, kurstaki, lambda-cyhalothrin, Leptomastix dactylopii, leptophos, levamisole, lineatin, lirimfos, looplure, lufenuron, Ivbenmixianan, lythidathion, m- cumenyl methylcarbamate, Macrolophus caliginosus, magnesium phosphide, malathion, maleic hydrazide, malonoben, Mamestra brassicae NPV, mancopper, mancozeb, mandestrobin, mandipropamid, maneb, mazidox, mebendazole, mecarbam, mecarphon, medlure, mefentrifluconazole, megatomoic acid, meloxicam, menazon, mepanipyrim, meperfluthrin, mephosfolan, mepiquat, mepronil, meptyldinocap, mesulfenfos, metaflumizone, metalaxyl, metalaxyl-M, metaldehyde, metam, metam-potassium, metam- sodium, Metaphycus helvolus, Metarhizium anisopliae var. acridum, Metarhizium anisopliae var. anisopliae, metarylpicoxamid, metconazole, methacrifos, methamidophos, methasulfocarb, methidathion, methiocarb, methiotepa, methocrotophos, methomyl, methoprene, methoquin-butyl, methothrin, methoxyfenozide, methyl apholate, methyl eugenol, methyl iodide, methyl neodecanamide, metiram, metofluthrin, metolcarb, metominostrobin, metoxadiazone, metrafenone, metyltetraprole, mevinphos, mexacarbate, MGK 264, milbemycin, milbemycin oxime, monocrotophos, morantel tartrate, morzid, moxidectin, muscalure, myclobutanil, myclozolin, Myrothecium verrucaria composition, N-[2-[2,4-dichloro-phenoxy]phenyl]-3- (difluoromethyl)-1-methyl-pyrazole-4-carboxamide, N-cyclopropyl-3-(difluoromethyl)-5-fluoro-N-[(2- isopropylphenyl)methyl]-1-methyl-pyrazole-4-carboxamide, nabam, naled, NC-170, nemadectin, Neodiprion sertifer NPV and N. lecontei NPV, niclosamide-olamine, nicotine, nicotine sulfate, nikkomycins, nitenpyram, nithiazine, nitrapyrin, nitrilacarb, nitrothal-isopropyl, norbormide, nornicotine, novaluron, noviflumuron, nuarimol, O,O,O',O'-tetrapropyl dithiopyrophosphate, octadeca-2,13-dien-1-yl acetate, octadeca-2,13-dien-1-yl acetate, octhilinone, ofurace, oleic acid, omethoate, orfralure, Orius spp., orysastrobin, osthol, ostramone, oxadixyl, oxamate, oxamyl, oxantel pamoate, oxasulfuron, oxathiapiprolin, oxfendazole, oxibendazole, oxine copper, oxolinic acid, oxpoconazole, oxycarboxin, oxydemeton-methyl, oxydeprofos, oxydisulfoton, oxytetracycline, oxytetracycline dihydrate, paclobutrazol, Paecilomyces fumosoroseus, paraoxon, parathion, parathion-methyl, parbendazole, pefurazoate, penconazole, pencycuron, penethamate, penflufen, penthiopyrad, permethrin, petroleum oils, PH 60-38, phenamacril, phenthoate, phorate, phosacetim, phosalone, phosfolan, phosglycin, phosmet, phosnichlor, phosphamidon, phosphocarb, phosphonic acid, phosphorus, phoxim, phoxim-methyl, phthalide, Phytoseiulus persimilis, picarbutrazox, picoxystrobin, pimobendan, pindone, piperalin, piperonyl butoxide, piprotal, pirimetaphos, pirimicarb, pirimiphos-methyl, polycarbamate, polynactin, polyoxin B, polyoxin d, potassium ethylxanthate, potassium hydroxyquinoline sulfate, praziquantel, precocene I, precocene II, precocene III, primidophos, probenazole, prochloraz, procymidone, profenofos, profluthrin, prohexadione, prohexadione-calcium, promacyl, promecarb, propamidine, propamocarb, propaphos, propargite, propetamphos, propiconazole, propineb, propionic acid, propoxur, propyl isomer, proquinazid, prothidathion, prothioconazole, prothiofos, prothoate, protrifenbute, pydiflumetofen, pymetrozine, pyraclofos, pyraclostrobin, pyrafluprole, pyrametostrobin, pyrantel pamoate, pyraoxystrobin, pyrapropoyne, pyraziflumid, pyrazophos, pyrazoxone, pyresmethrin, pyrethrin I, pyrethrin II, pyrethrins (natural products), pyrethroids (natural products), pyribencarb, pyridaben, pyridachlometyl, pyridalyl, pyridaphenthion, pyridin- 4-amine, pyrifenox, pyrifluquinazon, pyrimethanil, pyrimidifen, pyrimorph, pyriofenone, pyriprole, pyriproxyfen, pyrisoxazole, pyroquilon, quassia, quinalphos, quinalphos-methyl, quinoclamine, quinofumelin, quinonamid, quinothion, quinoxyfen, quintozene, R-1492, R-metalaxyl, Reynoutria sachalinensis extract, ribavirin, rotenone, ryanodine (ryania), sabadilla, schradan, scilliroside, seboctylamine, sedaxane, selamectin, sesamex, sesamolin, siglure, silafluofen, silthiofam, simeconazole, sodium tetrathiocarbonate, sophamide, sordidin, spinetoram, spinosad, spirodiclofen, spiromesifen, spirotetramat, spiroxamine, Steinernema bibionis, Steinernema carpocapsae, Steinernema feltiae, Steinernema glaseri, Steinernema riobrave, Steinernema riobravis, Steinernema scapterisci, Steinernema spp., streptomycin, streptomycin sesquisulfate, sulcatol, sulcofuron, sulfiram, sulfur, sulprofos, tar oils, tau- fluvalinate, TCMTB, TDE, tebuconazole, tebufenozide, tebufenpyrad, tebufloquin, tebupirimfos, tecnazene, teflubenzuron, tefluthrin, temephos, terallethrin, terbam, terbufos, tetrachlorvinphos, tetraconazole, tetradec-11-en-1-yl acetate, tetradifon, tetramethrin, tetramethylfluthrin, tetranactin, thiabendazole, thiacloprid, thiadiazole copper, thiamethoxam, thiapronil, thicrofos, thicyofen, thidiazuron, thifluzamide, thiocarboxime, thiocyclam, thiocyclam hydrogen oxalate, thiodicarb, thiofanox, thiometon, thiophanate, thiophanate-methyl, thioquinox, thiosultap, thiosultap-disodium, thiram, thuringiensin, tiadinil, tiamulin, tioxymid, tolclofos-methyl, tolfenpyrad, tolprocarb, tolylfluanid, tralomethrin, transpermethrin, tretamine, triadimefon, triadimenol, triarathene, triazamate, triazophos, triazoxide, tribufos, trichlorfon, trichlormetaphos-3, trichloronat, Trichogramma spp., triclabendazole, triclopyricarb, tricyclazole, tridemorph, trifenmorph, trifloxystrobin, triflumizole, triflumuron, triforine, trimedlure, trimedlure A, trimedlure B1 , trimedlure B2, trimedlure C, trimethacarb, trinactin, trinexapac, trinexapac-ethyl, trioxyflanilide, triprene, triticonazole, trunc-call, tulathromycin, Typhlodromus occidentalis, uniconazole, uredepa, validamycin, valifenalate, vamidothion, vaniliprole, veratridine, veratrine, verbutin, Verticillium lecanii, vinclozolin, XMC, xylenols, zeatin, zeta-cypermethrin, zhongshengmycin, zinc naphthenate, zinc thiazole, zineb, ziram, zolaprofos, and / or zoxamide.

A composition comprising components (A) and (B) as active ingredients can be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as discosed herein, such as a Streptomyces sp. Saigon413, deposited at the Westerdijk institute under accession number CBS149411 , and component (B) comprisea one or more of the following components:

2-(difluoromethyl)-N-[(3S)-3-ethyl-1 ,1-dimethyl-indan-4-yl]pyridine-3-carboxamide (this compound may be prepared from the methods described in WO 2014/095675), methyl 3-[(4-chlorophenyl)methyl]-2- hydroxy-1-methyl-2-(1 ,2,4-triazol-1-ylmethyl)cyclopentanecarboxylate (this compound may be prepared from the methods described in WO 2019/093522), methyl (2R)-2-[2-chloro-4-(4-chlorophenoxy)phenyl]-2- hydroxy-3-(1 ,2,4-triazol-1-yl)propanoate (this compound may be prepared from the methods described in WO 2019/093522), 5-[5-(difluoromethyl)-1 ,3,4-oxadiazol-2-yl]-N-[1-(2,6- difluorophenyl)cyclopropyl]pyrimidin-2-amine (this compound may be prepared from the methods described in WO 2021/255093), aminopyrifen (this compound may be prepared from the methods described in WO 2014/006945), dipymetitrone (this compound may be prepared from the methods described in WO 2011/138281), 1-[6-(difluoromethyl)-5-methyl-3-pyridyl]-4,4-difluoro-3,3-dimethyl-isoquinoline (this compound may be prepared from the methods described in WO 2017/016915), 1-[4-(difluoromethoxy)-2- methyl-phenyl]-2-(1 ,2,4-triazol-1-yl)-1-[1-(trifluoromethyl)cyclopropyl]ethanol (this compound may be prepared from the methods described in WO 2021/249800), 1-[2-chloro-4-(difluoromethoxy)phenyl]-2- (1 ,2,4-triazol-1-yl)-1-[1-(trifluoromethyl)cyclopropyl]ethanol (this compound may be prepared from the methods described in WO 2021/249800), 1-(5,6-dimethyl-3-pyridyl)-4,4-difluoro-3,3-dimethyl-isoquinoline (this compound may be prepared from the methods described in WO 2017/016915), N-methyl-4-[5- (trifluoromethyl)-l ,2,4-oxadiazol-3-yl]benzenecarbothioamide (this compound may be prepared from the methods described in WO 2015/185485), 2,2-difluoro-N-methyl-2-[4-[5-(trifluoromethyl)-1 ,2,4-oxadiazol-3- yl]phenyl]acetamide (this compound may be prepared from the methods described in WO 2017/178245), flufenoxadiazam, N-methyl-4-[5-(trifluoromethyl)-1 ,2,4-oxadiazol-3-yl]benzamide (this compound may be prepared from the methods described in WO 2015/185485), (Z,2E)-5-[1-(2,4-dichlorophenyl)pyrazol-3- yl]oxy-2-methoxyimino-N,3-dimethyl-pent-3-enamide (this compound may be prepared from the methods described in WO 2018/153707), (Z,2E)-5-[1-(4-chlorophenyl)pyrazol-3-yl]oxy-2-methoxyimino-N,3- dimethyl-pent-3-enamide (this compound may be prepared from the methods described in WO 2013/092224), methyl (2E)-2-methoxyimino-2-[3-methyl-2-[[(E)-1 -[4-(trifluoromethyl)-2- pyridyl]ethylideneamino]oxymethyl]phenyl]acetate (this compound may be prepared from the methods described in WO 2022/033906), (2E)-2-methoxyimino-N-methyl-2-[3-methyl-2-[[(E)-1-[4-(trifluoromethyl)-2- pyridyl]ethylideneamino]oxymethyl]phenyl]acetamide (this compound may be prepared from the methods described in WO 2022/033906), (2E)-2-[2-[[(E)-[3-(4-fluorophenyl)-1-methyl-prop-2- ynylidene]amino]oxymethyl]-3-methyl-phenyl]-2-methoxyimino-N-methyl-acetamide (this compound may be prepared from the methods described in WO 2021/249928), methyl (2E)-2-[2-[[(E)-[3-(4-fluorophenyl)-

1-methyl-prop-2-ynylidene]amino]oxymethyl]-3-methyl-phenyl]-2-methoxyimino-acetate (this compound may be prepared from the methods described in WO 2021/249928), 3-[2-(1-chlorocyclopropyl)-3-(3-chloro-

2-fluoro-phenyl)-2-hydroxy-propyl]imidazole-4-carbonitrile (this compound may be prepared from the methods described in WO 2016/156290), 3-[2-(1-chlorocyclopropyl)-3-(2-fluorophenyl)-2-hydroxy- propyl]imidazole-4-carbonitrile (this compound may be prepared from the methods described in WO 2016/156290), 2-[6-(4-bromophenoxy)-2-(trifluoromethyl)-3-pyridyl]-1-(1 ,2,4-triazol-1-yl)propan-2-ol (this compound may be prepared from the methods described in WO 2017/029179), 2-[6-(4-chlorophenoxy)-2- (trifluoromethyl)-3-pyridyl]-1-(1 ,2,4-triazol-1-yl)propan-2-ol (this compound may be prepared from the methods described in WO 2017/029179), 5-[5-(difluoromethyl)-1 ,3,4-oxadiazol-2-yl]-N-[1-(2- fluorophenyl)ethyl]pyrimidin-2-amine (this compound may be prepared from the methods described in WO 2021/255093), 5-[5-(difluoromethyl)-1 ,3,4-oxadiazol-2-yl]-N-[1-(3,5-difluorophenyl)ethyl]pyrimidin-2-amine (this compound may be prepared from the methods described in WO 2021/255093), N-[1-(2- fluorophenyl)cyclopropyl]-5-[5-(trifluoromethyl)-1 ,3,4-oxadiazol-2-yl]pyrimidin-2-amine (this compound may be prepared from the methods described in WO 2021/255093), 5-[5-(difluoromethyl)-1 ,3,4-oxadiazol-2-yl]- N-[1-(2,6-difluorophenyl)ethyl]pyrimidin-2-amine (this compound may be prepared from the methods described in WO 2021/255093), 2-(difluoromethyl)-5-[2-[1-(2,6-difluorophenyl)cyclopropoxy]pyrimidin-5-yl]- 1 ,3,4-oxadiazole (this compound may be prepared from the methods described in WO 2021/255093), 5-[5- (difluoromethyl)-l ,3,4-oxadiazol-2-yl]-N-[1-(2-fluorophenyl)cyclopropyl]pyrimidin-2-amine (this compound may be prepared from the methods described in WO 2021/255093), 5-[(4-bromo-2-methyl-phenyl)methyl]-

3-[3-(3-chloro-2-fluoro-phenoxy)-6-methyl-pyridazin-4-yl]-5,6-dihydro-4H-1 ,2,4-oxadiazine (this compound may be prepared from the methods described in WO 2021/255070), 3-[3-(3-cyclopropyl-2-fluoro-phenoxy)- 6-methyl-pyridazin-4-yl]-5-[(2,4-dimethylphenyl)methyl]-5,6-dihydro-4H-1 ,2,4-oxadiazine (this compound may be prepared from the methods described in WO 2021/255070), N-(2,2,2-trifluoroethyl)-2-[[4-[5- (trifluoromethyl)-l ,2,4-oxadiazol-3-yl]phenyl]methyl]oxazole-4-carboxamide (this compound may be prepared from the methods described in WO 2022/133114), ethyl 1-[[4-[5-(trifluoromethyl)-1 ,2,4-oxadiazol- 3-yl]phenoxy]methyl]pyrazole-4-carboxylate (this compound may be prepared from the methods described in WO 2022/133114), ethyl 1-[[4-[(Z)-2-ethoxy-3,3,3-trifluoro-prop-1-enoxy]phenyl]methyl]pyrazole-4- carboxylate (this compound may be prepared from the methods described in WO 2020/056090 and WO 2021/183707), ethyl 1-[[4-[[2-(trifluoromethyl)-1 ,3-dioxolan-2-yl]methoxy]phenyl]methyl]pyrazole-4- carboxylate (this compound may be prepared from the methods described in WO 2020/056090 and WO 2021/183707), methyl N-[[5-[4-(2,4-dimethylphenyl)triazol-2-yl]-2-methyl-phenyl]methyl]carbamate (this compound may be prepared from the methods described in WO 2020/097012), methyl N-[[5-[1-(2,6- difluoro-4-isopropyl-phenyl)pyrazol-3-yl]-2-methyl-phenyl]methyl]carbamate (this compound may be prepared from the methods described in WO 2020/097012), methyl N-[[5-[1-(4-cyclopropyl-2,6-difluoro- phenyl)pyrazol-3-yl]-2-methyl-phenyl]methyl]carbamate (this compound may be prepared from the methods described in WO 2020/097012), methyl 2-[2-chloro-4-(4-chlorophenoxy)phenyl]-2-hydroxy-3- (1 ,2,4-triazol-1-yl)propanoate (this compound may be prepared from the methods described in WO 2019/093522), 4,4,5-trifluoro-3,3-dimethyl-1-(3-quinolyl)isoquinoline, 5-fluoro-3,3,4,4-tetramethyl-1-(3- quinolyl)isoquinoline, 2-methoxy-N-[methoxy-[5-[5-(trifluoromethyl)-1 ,2,4-oxadiazol-3-yl]-2- thienyl]methyl]acetamide (this compound may be prepared from the methods described in WO 2020/256113), N-[methoxy-[5-[5-(trifluoromethyl)-1 ,2,4-oxadiazol-3-yl]-2-thienyl]methyl]-2-methyl- propanamide (this compound may be prepared from the methods described in WO 2020/256113), N- [methoxy-[5-[5-(trifluoromethyl)-1 ,2,4-oxadiazol-3-yl]-2-thienyl]methyl]butanamide (this compound may be prepared from the methods described in WO 2020/256113), 2-(difluoromethyl)-N-[(3R)-3-ethyl-1 ,1- dimethyl-indan-4-yl]pyridine-3-carboxamide (this compound may be prepared from the methods described in WO 2014/095675), 2-(difluoromethyl)-N-(3-ethyl-1 ,1-dimethyl-indan-4-yl)pyridine-3-carboxamide (this compound may be prepared from the methods described in WO 2014/095675), 2-(difluoromethyl)-N-(1 ,1 ,3- trimethylindan-4-yl)pyridine-3-carboxamide, (5R)-3-[3-(3-chloro-2-fluoro-phenoxy)-6-methyl-pyridazin-4- yl]-5-[(2-chloro-4-methyl-phenyl)methyl]-5,6-dihydro-4H-1 ,2,4-oxadiazine (this compound may be prepared from the methods described in WO 2020/127780, WO 2021/255070), (5S)-3-[3-(3-chloro-2-fluoro- phenoxy)-6-methyl-pyridazin-4-yl]-5-[(2-chloro-4-methyl-phenyl)methyl]-5,6-dihydro-4H-1 ,2,4-oxadiazine (this compound may be prepared from the methods described in WO 2020/127780, WO 2021/255070), 3- [3-(3-chloro-2-fluoro-phenoxy)-6-methyl-pyridazin-4-yl]-5-[(2-chloro-4-methyl-phenyl)methyl]-5,6-dihydro- 4H-1 ,2,4-oxadiazine (this compound may be prepared from the methods described in WO 2020/127780, WO 2021/255070).

A composition comprising components (A) and (B) as active ingredients, can be a composition wherein component (A) comprises a Streptomyces chrestomyceticus as disclosed herein, such as a Streptomyces sp. Saigon413, deposited at the Westerdijk institute under accession number CBS149411 , and component (B) comprises one or more of the following components: 2-[(2,6-difluoro-4-pyridyl)-(2-methylpropanoyl)amino]-N-(2,2-dimethylcyclobutyl)-5-methyl- thiazole-4-carboxamide, or the (R) or (S) enantiomer or mixtures thereof (this compound may be prepared from the methods described in WO2017207362A1 , WO2019105933A1 , W02020109511A1 , WO2021244952A1); 2-[(2,6-difluoro-4-pyridyl)-(tetrahydropyran-4-carbonyl)amino]-N-(2,2- dimethylcyclobutyl)-5-methyl-thiazole-4-carboxamide, or the (R) or (S) enantiomer or mixtures thereof (this compound may be prepared from the methods described in WO2017207362A1 , WO2019105933A1 , W02020109511A1 , WO2021244952A1); 2-[cyano-(2,6-difluoro-4-pyridyl)amino]-5-methyl-N- spiro[3.4]octan-3-yl-thiazole-4-carboxamide, or the (R) or (S) enantiomer or mixtures thereof (this compound may be prepared from the methods described in WO2017207362A1 , WO2019105933A1 , W02020109509A1); 2-[cyano-(2,6-difluoro-4-pyridyl)amino]-N-(2,2-dimethylcyclobutyl)-5-methyl-thiazole- 4-carboxamide, or the (R) or (S) enantiomer or mixtures thereof (this compound may be prepared from the methods described in WO2017207362A1 , WO2019105933A1 , W02020109509A1); 2-[(2,6-difluoro-4- pyridyl)-(2-methoxyacetyl)amino]-N-(2,2-dimethylcyclobutyl)-5-methyl-thiazole-4-carboxamide, or the (/?) or (S) enantiomer or mixtures thereof (this compound may be prepared from the methods described in WO2017207362A1 , WO2019105933A1 , W02020109511A1 , WO2021244952A1); 2-[acetyl-(2,6-difluoro- 4-pyridyl)amino]-5-methyl-N-spiro[3.4]octan-3-yl-thiazole-4-carboxamide, or the (/?) or (S) enantiomer or mixtures thereof (this compound may be prepared from the methods described in WO2017207362A1 , WO2019105933A1 , W02020109511A1 , WO2021244952A1); N-methoxy-N-[[4-[5-(trifluoromethyl)-1 ,2,4- oxadiazol-3-yl]phenyl]methyl]cyclopropanecarboxamide (this compound may be prepared from the methods described in WO 2017/055473); methyl (Z)-2-(5-cyclopentyl-2-methyl-phenoxy)-3-methoxy-prop- 2-enoate (this compound may be prepared from the methods described in W02020/193387); methyl (Z)-2- (5-cyclohexyl-2-methyl-phenoxy)-3-methoxy-prop-2-enoate (this compound may be prepared from the methods described in W02020/193387); N-[(1 R)-1-benzyl-1 ,3-dimethylbutyl]-8-fluoroquinoline-3- carboxamide (this compound may be prepared from the methods described in WO2017/153380); N-[(1 S)- 1-benzyl-1 ,3-dimethylbutyl]-8-fluoroquinoline-3-carboxamide (this compound may be prepared from the methods described in WO2017/153380); 2-[(2,6-difluoro-4-pyridyl)-(oxetane-3-carbonyl)amino]-N-(2,2- dimethylcyclobutyl)-5-methyl-thiazole-4-carboxamide, or the (/?) or (S) enantiomer or mixtures thereof (this compound may be prepared from the methods described in WO2017207362A1 , WO2019105933A1 , W02020109511A1 , WO2021244952A1); 2-[acetyl-(2,6-difluoro-4-pyridyl)amino]-N-(2,2- dimethylcyclobutyl)-5-methyl-thiazole-4-carboxamide, or the (/?) or (S) enantiomer or mixtures thereof (this compound may be prepared from the methods described in WO2017207362A1 , WO2019105933A1 , W02020109511A1 , WO2021244952A1); N,2-dimethoxy-N-[[4-[5-(trifluoromethyl)-1 ,2,4-oxadiazol-3- yl]phenyl]methyl]propanamide, or the (/?) or (S) enantiomer or mixtures thereof (this compound may be prepared from the methods described in WO 2017/055473).

FIGURES

Figure 1. 1 D ¹H NMR spectrum of the compound I in D2O at 600 MHz

Figure 2. 1 D ¹³C NMR spectrum of the compound I in D2O at 600 MHz

Figure 3. 2D Dept Edited 1 H-13C HSQC NMR Spectrum of the compound I in D2O.at 600 MHz showing the positive (CH) signals

Figure 4. 2D Dept Edited 1 H-13C HSQC NMR Spectrum of the compound I in D2O.at 600 MHz showing the negative (CH2) signals

Figure 5. Spectrum of light absorption (UV-VIS) 200-400nm of a lipopeptide according to Formula II (a), Formula II (b) or Lipopeptin A

Figure 6. LC-ESI-MS/MS spectrum of precursor 1204.6 m/z (M+H)⁺ for a lipopeptide of Formula ll(a) depicting fragment peaks consistent with amino acids: aspartic acid, hydroxy-glutamine, serine, methylasparagine, methyl-phenylalanine Figure 7. LC-ESI-MS/MS/MS spectrum of precursor 294.2 m/z for a lipoeptide of Formula 11 (a) depicting peaks consistent with the molecule C14H25-OH2-C4H5ON

Figure 8. The upfield region of the 1 D ¹H NMR spectrum of a lipopeptide according to Formula ll(a) in CDsOD at 600 MHz

Figure 9. The downfield region of the 1 D ¹H NMR spectrum of a lipopeptide according to Formula 11 (a) in CD3OD at 600 MHz

Figure 10. Spectrum of light absorption (UV-VIS) 200-500nm of polyene compound

EXAMPLES

Example 1.1. Source and fermentation

Fermentation of Streptomyces sp.

Streptomyces sp. Saigon413, isolated in Vietnam before 1961 , was deposited at the Westerdijk institute, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands under accession number CBS149411 . The deposit was made by Syngenta Ltd., Jealott’s Hill Research International Centre, Bracknell, Berkshire, RG42 6EY, UK under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure.

The Streptomyces was cultivated in Erlenmeyer flasks with a liquid medium consisting of (g / I) casein hydrolysate 10, glucose 40, K2HPO4 1.25, soytone 2, tryptone, 8 and incubated at 28°C in an incubator shaking 150 rpm with 25 mm throw for 4 days.

Large scale fermentations

For large scale production, standard procedures were applied for cultivating Streptomyces chrestomyceticus CBS149411 to high cell density using fed-batch fermentation. After harvesting, the broth was spray dried or freeze dried according to methods known to a person skilled in the art.

The final product (TGAI) after spray- or freeze drying had a cell count of 1*10⁵ to 1*10¹³ CFU/g dry mass. TGAI: technical grade active ingredient (unformulated product).

For the in vitro tests (Example 2) with a second compound, spray-dried or freeze-dried S. chrestomyceticus CBS149411 (Streptomyces sp. Saigon 413) TGAI was used.

Example 1.2. OD formulation

For foliar treatments of diseases on different crops (Example 3), an oil dispersion (OD) of spray-dried or freeze dried fermentation broth Streptomyces Saigon 413 TGAI was prepared.

An OD40% w/w was prepared of 40% dried fermentation broth in a vegetable oil containing an emulsifier, such as a surfactant, using general formulation technology as disclosed on Croda Crop Care, the Nouryon formulator toolbox and in: Formulation of Microbial Biopesticides: Beneficial microorganisms, nematodes and seed treatments (412 p., 6 December 2012) eds. Burges H.D., Springer, ISBN 978-94-011-4926-6.

Example 1.3. Isolation of 16S rDNA, genomic DNA and species identification

Genomic DNA was isolated from Streptomyces sp. Saigon413 using the method described in Kutchma et al. (1998) Biotechniques 24(3):452-457. The 16S rRNA gene was amplified using universal 16S primers and sequenced using Sanger sequencing. The 16S rRNA of Streptomyces sp. Saigon413 is shown in SEQ ID NO: 1.

Whole genome sequencing, using the genomic DNA from Streptomyces sp. Saigon413, was completed using both Pacific Biosciences and Illumina sequencing technologies. The genome was assembled using HFAP4 and polished with Pilon using the Illumina reads.

Example 1.4. Analysis of Streptimidone, cyclothiazomycin C and malonomicin

The structure of cyclothiazomycin C is disclosed on p. 3 of WO2015191789 and can be extracted and analysed according to the method discosed in Wang et al. (2010) Appl. Environmental Microbiology, Vol. 76, No. 7 p.2336. Malonomicin can be extracted and analysed according to the method disclosed in Example I (B) of W02006/078939. Streptimidone can be extracted and isolated according to the method as disclosed in Lee et al. J. of Antibiotics (2020) 73: p. 184-188, including the supplementary information.

Example 1.5. Oligosaccharide compound I

Purification of the oligosaccharide compound I

Whole broth Streptomyces chrestomyceticus CBS149411 (Streptomyces sp. Saigon413), was centrifuged to produce an aqueous extract and a pellet. The aqueous extract was freeze dried. The material was resuspended in a minimal volume of water and partitioned with ethyl acetate to remove lipophilic components. The aqueous suspension was retained and freeze dried and resuspended in a minimal volume of water before being applied to an activated charcoal column.

The column was washed with water and eluted with water:acetone (50:50).

The compound I was further purified by Hydrophilic Interaction Liquid Chromatography (HILIC) using Mass guided fractionation and ELSD detector. Using for example Waters XBridge Amide, 5 micron, 30x100mm using a gradient of acetonitrile and 10mM Ammonium Acetate.

Characterisation of oligosaccharide compound I

The compound I was determined in the purified fermentation broth according to the methods disclosed below.

Molecular composition and total molecular mass The molecular composition and total molecular mass was C53H90N2O44, and 1458.487 g, respectively which were determined using MS-MS and NMR spectroscopy as disclosed below.

Solubility

The solubility of the oligosaccharide compound I in water, pH 7.01 , was >10’000 ppm, and in DMSO it was > 9772 ppm.

MS-MS Spectrometry and liquid chromatrography

Spectra were recorded on an Orbitrap ID-X Tribrid Mass Spectrometer from Thermo Scientific equipped with an OptaMax NG Heated Electrospray Source (Spray Voltage: Static, Polarity Ion (V): 3400 (Positive ion mode) & 2400 (Negative ion mode), Sheath Gas (Arb): 40, Aux Gas (Arb): 5, Sweep Gas (Arb): 1 , Ion Transfer Tube Temperature: 350 °C, Vaporizer Temperature: 350 °C). The Scan Parameters were as follows;

Experiment 1 : MS OT (Orbitrap Resolution: 60,000, Scan Range (m/z): 200 to 2000, RF Lens (%): 60, AGO Target: Standard, Maximum Injection Time Mode: Auto, Microscans: 1 , Data Type: Profile, Polarity: Both), Experiment 2: tMS2 OT CID (MSn Level (n): 2, Isolation Window (m/z): 1.6, Activation Type: CID, CID Collision Energy (%): 30, Detector Type: Orbitrap, Orbitrap Resolution: 30,000, RF Lens (%): 60, Polarity: Negative). The mass spectrometer was connected to a Vanquish Flex UHPLC from Thermo Scientific using a Vanquish Split Sampler FT, Vanquish Binary Pump F, Vanquish Column Compartment H, Vanquish Diode Array Detector FG and Vanquish Charged Aerosol Detector. Liquid Chromatography Conditions included: Thermo Scientific Hypercarb™ Porous Graphitic Carbon column 5pm 4.6x50mm, P.N. 35005-054630. Temp: 40°C, DAD wavelength range: 250 to 260nm, Solvent gradient: Solvent A: H2O with 0.1% formic acid, Solvent B: CH3CN with 0.1% formic acid, gradient: 0 min 1% B, 99% A; 4. OOmin 50% B, 50% A; 4.25min 100% B; 4.50min 100% B; 4.95min 1% B, 99% A; 6. OOmin 1% B, 99% A, Flow rate: 1.0ml/min, Injection volume: 2 uL, Total run time: 6.0min.

NMR Spectroscopy

NMR spectra were recorded on a Bruker AVIII 600 NMR spectrometer, equipped with a 5 mm Bruker (¹H/¹⁹F)/¹³C/¹⁵N TCI cryoprobe fitted with Z gradients, using standard Bruker pulse sequences. Samples were dissolved in D2O, and the spectra were recorded at 300° K and referenced to acetone at 2.225 ppm for ¹H and 31 .07 ppm for ¹³C. Figures 1 to 4 show NMR spectra of the compound of the present invention. The one bond ¹H-¹³C correlation spectrum contains peaks corresponding to 1 methyl (CH3) and 40 methine (CH) groups (listed in Table 1) and 9 methylene (CH2) groups (listed in Table 2).

In addition, the 1 D ¹³C spectrum contains signals from 3 quaternary carbons at 104.7, 159.3 and 175.2 ppm (± 0.1). Table 1. Methyl and methine signals in the one bond ¹H-¹³C correlation spectrum of the oligosaccharide compound I together with multiplicity information for protons resolved in the 1 D ¹H spectrum.

Table 2. Methylene signals in the one bond ¹H-¹³C correlation spectrum of the compound I together with multiplicity information for protons resolved in the 1 D ¹H spectrum.

Example 1.6. Lipopeptide compound of Formula II Purification of a lipopeptide according to Formula II (Formula ll(a) and Formula ll(b))

The mycelia from fermentation broth from Streptomyces sp. Saigon 413, was separated via centrifugation and the supernatant was treated with butanol. The butanol was removed and the extract partitioned between water and ethyl acetate. The lipopeptides were purified from the ethyl acetate fraction by preparative reverse phase (C18) HPLC. The lipopeptides were relatively aploar and elute in the higher organic fraction in a gradient system with 0.1% formic acid and acetonirile (0.1% formic acid). A gradient of 60% Aqueous to 40% Aqueous with the above solvents allowed separation of a lipopeptide compound according to Formula 11 (a) and Formula I l(b).

Compounds were detected by UV-VIS (Figure 5), mass spectrometry (Figures 6 and 7) and NMR spectrometry (Figures 8 ad 9).

Characterisation of a lipopeptide of Formula II

Liquid Chromatography and High-Resolution Mass Spectrometry

Spectra were recorded on an Orbitrap ID-X Tribrid Mass Spectrometer from Thermo Scientific equipped with an OptaMax NG Heated Electrospray Source (Spray Voltage: Static, Polarity Ion (V): 3400 (Positive ion mode) & 2400 (Negative ion mode), Sheath Gas (Arb): 40, Aux Gas (Arb): 5, Sweep Gas (Arb): 1 , Ion Transfer Tube Temperature: 350 °C, Vaporizer Temperature: 350 °C). The Scan Parameters were as follows;

Experiment 1 : MS OT (Orbitrap Resolution: 50,000, Scan Range (m/z): 200 to 2000, RF Lens (%): 60, AGO Target: Standard, Maximum Injection Time Mode: Auto, Microscans: 1 , Data Type: Profile, Polarity: Both), Experiment 2: tMS2 OT CID (MSn Level (n): 2, Isolation Window (m/z): 1.0, Activation Type: CID, CID Collision Energy (%): 30, Detector Type: Orbitrap, Orbitrap Resolution: 30,000, RF Lens (%): 60, Polarity: Positive),

Experiment 3: tMS2 OT HCD (MSn Level (n): 2, Isolation Window (m/z): 1 .0, Activation Type: HCD, HCD Collision Energy (%): 30, Detector Type: Orbitrap, Orbitrap Resolution: 30,000, RF Lens (%): 60, Polarity: Positive), Experiment 4: tMS3 OT HCD (MSn Level (n): 3, Isolation Window (m/z): 1.6, Activation Type: HCD, HCD Collision Energy (%): 30, MS2 Isolation Window (m/z): 2, MS2 Activation Type: HCD, MS2 HCD Collision Energy (%): 30, Detector Type: Orbitrap, Orbitrap Resolution: 30,000, RF Lens (%): 60, Polarity: Positive). The mass spectrometer was connected to a Vanquish Flex UHPLC from Thermo Scientific using a Vanquish Split Sampler FT, Vanquish Binary Pump F, Vanquish Column Compartment H, Vanquish Diode Array Detector FG and Vanquish Charged Aerosol Detector.

Liquid Chromatography Conditions included: Waters ACQUITY UPLC C18 column 1 .7pm 3.0x50mm, P.N. 186004660. Temp: 40°C, DAD wavelength range: 250 to 260nm, Solvent gradient: Solvent A: H2O with 0.1 % formic acid, Solvent B: CH3CN with 0.1 % formic acid, gradient: 0 min 10% B, 90% A; 4. OOmin 90% B, 10% A; 4.25min 90% B, 10% A; 4.50min 10% B, 90% A; 5.00min 10% B, 90% A, Flow rate: 1.0ml/min, Injection volume: 2 uL, Total run time: 5.0min. A purified fermentation broth as described above was injected. Figures 6 and 7 show LC-ESI-MS/MS/MS spectra of a compound of Formula II (a).

NMR Spectroscopy

NMR spectra were recorded on a Bruker AVIII 600 NMR spectrometer, equipped with a 5 mm Bruker (¹H/¹⁹F)/¹³C/¹⁵N TCI cryoprobe fitted with Z gradients, using standard Bruker pulse sequences. Samples were dissolved in CDsOD, and the spectra were recorded at 300° K and referenced to the residual solvent signal at 3.31 ppm for ¹H. Figures 8 and 9 each cover half of the 1 H NMR spectrum of a compound according to Formula I l(a).

Molecular composition and mass

The molecular composition and mass of a lipopeptide according to Formula I l(a) and Formula I l(b) was determined using the results of liquid chromatography and high-resolution mass spectrometry as disclosed above. The lipopeptide compounds of Formula 11 (a) and 11 (b) have the following composition.

Formula II (a) Lipopeptide 1204: Molecular composition C55H85N11 O19 and exact mass of 1203.602. Formula II (b) Lipopeptide 1218: Molecular composition C56H87N11 O19 and exact mass of 1217.618.

Solubility

The solubility of a compound of Formula ll(a), Formula II (b) and Lipopeptin A was determined in water and

DMSO:

Compound solvent (pH) solubility (ppm)

Formula II (a) Lipopeptide 1204 water (2.08) 21 .4

Formula II (a) Lipopeptide 1204 water (5.55) >10’000

Formula II (a) Lipopeptide 1204 DMSO >10’000

Formula II (b) Lipopeptide 1218 DMSO >10’000

Example 1.7. Polyene compound

Purification of polyene compound

A spray dried sample from a culture of S. chrestomycetiucs CBS149411 (Streptomyces sp. Saigon 413) was washed with water. The solid residue was extracted twice with isopropanol and the isopropanol was removed. The resulting solid was purified by preparative reverse phase (C18) HPLC using an acetonitrile:water gradient. Further purification was conducted by preparative reverse phase HPLC using a Zorbax C8 column and eluting with an acetonitrile:water gradient.

The compound was detected by UV-VIS (Figure 10).

Characterisation of polyene compound

Liquid Chromatography and High-Resolution Mass Spectrometry Spectra were recorded on an Orbitrap ID-X Tribrid Mass Spectrometer from Thermo Scientific equipped with an OptaMax NG Heated Electrospray Source (Spray Voltage: Static, Polarity Ion (V): 3400 (Positive ion mode) & 2400 (Negative ion mode), Sheath Gas (Arb): 40, Aux Gas (Arb): 5, Sweep Gas (Arb): 1 , Ion Transfer Tube Temperature: 350 °C, Vaporizer Temperature: 350 °C). The Scan Parameters were as follows;

Experiment 1 : MS OT (Orbitrap Resolution: 50,000, Scan Range (m/z): 200 to 2000, RF Lens (%): 60, AGO Target: Standard, Maximum Injection Time Mode: Auto, Microscans: 1 , Data Type: Profile, Polarity: Both), Experiment 2: tMS2 OT CID (MSn Level (n): 2, Isolation Window (m/z): 1.0, Activation Type: CID, CID Collision Energy (%): 30, Detector Type: Orbitrap, Orbitrap Resolution: 30,000, RF Lens (%): 60, Polarity: Positive),

Experiment 3: tMS2 OT HCD (MSn Level (n): 2, Isolation Window (m/z): 1 .0, Activation Type: HCD, HCD Collision Energy (%): 30, Detector Type: Orbitrap, Orbitrap Resolution: 30,000, RF Lens (%): 60, Polarity: Positive), Experiment 4: tMS3 OT HCD (MSn Level (n): 3, Isolation Window (m/z): 1.6, Activation Type: HCD, HCD Collision Energy (%): 30, MS2 Isolation Window (m/z): 2, MS2 Activation Type: HCD, MS2 HCD Collision Energy (%): 30, Detector Type: Orbitrap, Orbitrap Resolution: 30,000, RF Lens (%): 60, Polarity: Positive).

The mass spectrometer was connected to a Vanquish Flex UHPLC from Thermo Scientific using a Vanquish Split Sampler FT, Vanquish Binary Pump F, Vanquish Column Compartment H, Vanquish Diode Array Detector FG and Vanquish Charged Aerosol Detector.

Liquid Chromatography Conditions included: Waters ACQUITY UPLC C18 column 1 .7pm 3.0x50mm, P.N. 186004660. Temp: 40°C, DAD wavelength range: 250 to 260nm, Solvent gradient: Solvent A: H2O with 0.1 % formic acid, Solvent B: CH3CN with 0.1 % formic acid, gradient: 0 min 10% B, 90% A; 4. OOmin 90% B, 10% A; 4.25min 90% B, 10% A; 4.50min 10% B, 90% A; 5.00min 10% B, 90% A, Flow rate: 1.0ml/min, Injection volume: 2 uL, Total run time: 5.0min.

A purified fermentation broth as described under section 1.8.1 was injected.

The key peaks observed were:

Negative ion: C67H114NO25 [M-H]’ Expected: 1332.7685, Observed: 1332.7679

Positive ion: C67H114NO24 [M-H2O+H]⁺ Expected: 1316.7725, Observed: 1316.7709

Positive ion: C67H115NO24 [M-H2O+2H]²⁺ Expected: 658.8899, Observed: 658.8895

Positive ion: C67H113NO23 [M-2(H2O)+2H]²⁺ Expected: 649.8846, Observed: 649.8843

Positive ion: C67H111 NO22 [M-3(H2O)+2H]²⁺ Expected: 640.8793, Observed: 640.8790

In a second experiment, Liquid Chromatography Conditions included: Kinetex Polar C18 column 100A 4.6x100mm, P.N. H17-055453. Temp: 40°C, DAD wavelength range: 250 to 260nm, Solvent gradient: Solvent A: H2O with 0.1 % formic acid, Solvent B: CH3CN with 0.1 % formic acid, gradient: Omin 10% B, 90% A; 1min 10% B, 90% A; 6.50min 95% B, 5% A; 8.00min 95% B, 5% A; 9.00min 10% B, 90% A; 10. OOmin 10% B, 90% A, Flow rate: 1.0ml/min, Injection volume: 5 uL, Total run time: 10. Omin. Under these conditions, the polyene compound had a retention time of 5.55-5.57 minutes.

The observed mass of the polyene compound was the same as in the previous Liquid chromatography run (results not shown).

Molecular composition and mass

The molecular composition and mass of the polyene compound was determined using the results of liquid chromatography and high-resolution mass spectrometry as disclosed above. The polyene compound has a molecular composition C67H115NO25 and exact mass of 1333.7758.

Solubility

The solubility of a compounds was determined in DMSO:

Compound solvent (pH) solubility (ppm)

Polyene compound DMSO >10’000

Example 2. In vitro efficacy of a mixture of Streptomyces chrestomyceticus CBS149411 and a second compound

Example 2.1 In vitro efficacy of a mixture of Streptomyces chrestomyceticus CBS149411 and a second compound against Pythium ultimum, Fusarium culmorum, Monographella nivalis syn. Microdochium nivale, Fusarium nivale, Septoria tritici, Glomerella lagenarium syn. Colletotrichum lagenarium, Botrytis cinerea

The following examples show the efficacy of a mixture of spray dried or freeze dried material of Streptomyces chrestomyceticus CBS149411 (compound A) and a second compound (compound B) against Pythium ultimum, Fusarium culmorum, Monographella nivalis syn. Microdochium nivale, Fusarium nivale, Zymoseptoria (Septoria tritici), Glomerella lagenarium syn. Colletotrichum lagenarium, and Botrytis cinerea.

The chemical compounds were from Syngenta or supplied by other commercial vendors.

An efficacy of 0 means that the growth level of the pathogen corresponds to that of the untreated control; an efficacy of 100 means that the growth level of the pathogen was fully inhibited.

Efficacy against Pythium ultimum

Mycelial fragments of the pathogen, prepared from a fresh liquid culture, were directly mixed into nutrient broth (potato dextrose broth). After placing a (DMSO) solution of the test compounds into a microtiter plate (96-well format), the nutrient broth containing the mycelial fragments was added. The test plates were incubated at 24°C and the inhibition of growth was determined after 48 hrs.

The following mixture compositions (A:B) at the reported concentration (in ppm) gave at least 80% efficacy in this test:

Table 3.

Efficacy against Fusarium culmorum

Conidia of the fungus from cryogenic storage were directly mixed into nutrient broth (potato dextrose broth). A DMSO solution of the test compounds was placed into a microtiter plate (96-well format) and the nutrient broth containing the fungal spores was added to it. The test plates were incubated at 24°C and the inhibition of growth was determined after 48 hrs.

The following mixture compositions (A:B) at the reported concentration (in ppm) gave at least 80% efficacy in this test: Table 4.

Efficacy against Monographella nivalis syn. Microdochium nivale, Fusarium nivale

Conidia of the fungus from cryogenic storage were directly mixed into nutrient broth (potato dextrose broth). A DMSO solution of the test compounds was placed into a microtiter plate (96-well format) and the nutrient broth containing the fungal spores was added to it. The test plates were incubated at 24°C and the inhibition of growth was determined after 72 hrs.

The following mixture compositions (A:B) at the reported concentration (in ppm) gave at least 80% efficacy in this test: Table 5.

Efficacy against Septoria tritici

Conidia of the fungus from cryogenic storage were directly mixed into nutrient broth (potato dextrose broth). A DMSO solution of the test compounds was placed into a microtiter plate (96-well format) and the nutrient broth containing the fungal spores was added to it. The test plates were incubated at 24°C and the inhibition of growth was determined after 72 hrs.

The following mixture compositions (A:B) at the reported concentration (in ppm) gave at least 80% efficacy in this test:

Table 6

Efficacy against Glomerella lagenarium svn. Colletotrichum lagenarium

Conidia of the fungus from cryogenic storage were directly mixed into nutrient broth (potato dextrose broth). A DMSO solution of the test compounds was placed into a microtiter plate (96-well format) and the nutrient broth containing the fungal spores was added to it. The test plates were incubated at 24°C and the inhibition of growth was determined after 72 hrs.

The following mixture compositions (A:B) at the reported concentration (in ppm) gave at least 80% efficacy in this test: Table ?.

Efficacy against Botrytis cinerea

Conidia of the fungus from cryogenic storage were directly mixed into nutrient broth (Vogel’s minimal media). A DMSO solution of the test compounds was placed into a microtiter plate (96-well format) and the nutrient broth containing the fungal spores was added to it. The test plates were incubated at 24°C and the inhibition of growth was determined after 72 hrs.

The following mixture compositions (A:B) at the reported concentration (in ppm) gave at least 80% efficacy in this test:

Table 8.

Example 2.2. In vitro efficacy of a mixture of Streptomyces CBS149411 and cyclobutrifluram against Fusarium graminearum

Fusarium graminearum strain K-6102 fresh colonies were produced on PDA containing LB medium + 2% agar. Agar plugs were taken from Fusarium graminearum grown colonies with autoclaved Pasteur pipettes’ bottoms (0 6mm) and one plug was placed in the center of agar plates containing a composition of spray dried or freeze dried TGAI of S. chrestomycticus CBS149411 and / or cyclobutrifluram. After incubation at 22°C for 5 days, the mycelium growth (diameter) was measured with a ruler and % efficacy was calculated. The expected efficacy (E exp) of the mixture was calculated using the Colby formula (1): E exp = A + B - (AxB/100), A and B are the control levels given by the solo treatments.

For synergy calculation, the ratio (SF, synergy factor) between the observed experimental efficacy of the mixture (E obs) and the expected efficacy of the mixture (E exp) was calculated as follows: SF = E obs-^-E exp. If SF > 1 , this indicates synergism.

Efficacy of cyclobutrifluram and S. chrestomyceticus CBS 149411 against Fusarium graminearum

Efficacy of mixtures of cyclobutrifluram and S. chrestomycticus CBS149411 against Fusarium graminearum

The results above show synergistic interaction between cyclobutrifluram and a composition comprising S. chrestomycticus CBS149411 as mixture partners against Fusarium graminearum using an in vitro bioassay. Example 3.

Control of foliar diseases with Streptomyces chrestomyceticus CBS 149411 TGAI in mixture with chemical products against Zymoseptoria tritici, Puccinia recondita sp. tritici and Pyricularia oryzae on planta.

Wheat or rice seedlings 14days old were sprayed with S. chrestomyceticus CBS149411 formulated as OD40% alone, or mixed with a second product before spraying the plants and subsequent infection with a fungal pathogen.

The efficacy of the mixture was also compared to the efficacy of the solo second compound product. The mixture of the two components was done in water before the mixture was loaded into the tank of the spray equipment. The spray volume was 200L/ha, the application was executed with a boom sprayer-type equipment in a glasshouse setting. Both components were mixed at different use rates, resulting in a multitude of different mixture ratios. For examples, CBS149411 formulated as OD40% was sprayed at a use rate of 500g/ha (active ingredient), and a second product at a use rate of 100g/ha (active ingredient), resulting in a ratio of 5:1 (CBS 149411 : 2nd compound). Mixtures resulting in 30% or more reduction of disease symptoms were seen as effective to provide a means for disease control or disease reduction.

Mixtures were also analyzed for the presence of a synergistic interaction between the two components to control the target pathogen.

Synergy of two products was assumed when the observed disease control was at least 30%, and higher than the expected disease control from the same mixture based on the prediction using efficacy of solo components and the equation from COLBY.

Compounds tested in mixtures with Streptomyces sp. Saigon413, CBS149411 formulated as OD40% were chosen to cover a large diversity of different mode of action. The FRAC Code List 2023 provides a comprehensive overview of different mode of action used to control fungal and bacterial diseases on crop plants (FRAC: Fungicide resistance action committee, is a specialist technical group of Crop Life international).

Chemical compounds listed in Table 9 were purchased from commercial vendors such as the following: Adama: ADAMA Agricultural Solutions Ltd., Golan Street Airport City, 7015103 ISRAEL BASF: BASF SE, 67056 Ludwigshafen, GERMANY

Bayer: Bayer AG, Crop Science Division, Alfred-Nobel-Str. 50, 40789 Monheim am Rhein, GERMANY Corteva: Corteva agriscience, 9330 Zionsville Road, Indianapolis, IN 46268, USA

Mitsui Chemicals: Mitsui Chemicals, Inc, Tokyo Midtown Yaesu, Yaesu Central Tower, 2-2-1 Yaesu, Chuo-ku, Tokyo 104-0028, JAPAN

Nippon Soda: Nippon Soda Co., Ltd., 2-7-2, Marunouchi, Chiyoda-ku, Tokyo 100-7010, JAPAN Sumitomo: Sumitomo chemical company, limited, Tokyo Nihombashi Tower, 2-7-1 , Nihonbashi, Chuo-ku, Tokyo 103-6020, JAPAN Syngenta: Syngenta Crop Protection AG, Rosentalstr. 67, 4058 Basel, SWITZERLAND

Zhejiang Xinnong Chemical: Zhejiang XinNong Chemical Co., Ltd, 11 F , Poly Center, No.277 Xintang Road, Jianggan District, Hangzhou, Zhejiang, P.R. CHINA

Where available, active ingredients are also identified by a CAS number (CAS: Chemical Abstracts Service, a division of the American Chemical Society (2540 Olentangy River Road, Columbus, Ohio 43202 USA).

Table 9. List of compounds tested in mixture with Streptomyces sp. Saigon 413, CBS149411 . a) N-(2,4-dimethyl-1-phenylpentan-2-yl)-8-fluoroquinoline-3-carboxamide (CAS 2132414-04-9) was produced as disclosed in WO2017/153380 b) Feneptamidoquin, is defined as a composition comprising N-[(2RS)-2,4-dimethyl-l- phenylpentan-2-yl]-8-fluoroquinoline-3-carboxamide, containing 80-100% of the R-enantiomer, or N-[(lRS)-l,3-dimethyl-l-(phenylmethyl)butyl)]-8-fluoro-3-quinolinecarboxamide, containing

80-100% of the R-enantiomer.

Methods:

Puccinia recondita (EPPO code: PUCCRE) 14 days old wheat plants variety Arina were inoculated by spraying them with a spore suspension one day after application (spore suspension at 80,000 spores per ml in water supplemented with Tween20 at 0.1%). After an incubation period of 1 day at 20° C and 95% relative humidity, the inoculated test plants were kept at 20° C and 60% relative humidity in a greenhouse. The percentage leaf area covered by disease was assessed visually when an appropriate level of disease appeared on untreated check plants (9 - 12 days after infection).

Zymoseptoria tritici (EPPO code: SEPTTR): 14 days old wheat plants variety Riband were inoculated by spraying a spore suspension on them one day after application (1 .5 Mio spores per ml in water supplemented with 0.01 % Tween20). After an incubation period of 4 days at 22°C/21 °C (day/night) and 95% relative humidity, the inoculated test plants were kept at 22°C/21°C (day/night) and 70% relative humidity in a greenhouse. Efficacy was assessed directly when an appropriate level of disease appeared on untreated check plants (14 - 19 days after application). The isolate used is a recent field isolate expressing tolerance to DMI fungicides, SDHI fungicides and Qol fungicides.

Pyricularia oryzae (EPPO code: PYRIOR): 3 week old rice plants cv. Balilla were treated with the formulated test compound in a spray chamber. Two days after application rice plants were inoculated by spraying a spore suspension (1 x 10⁵ conidia/ml) on the test plants. After an incubation period of 6 days at 25° C and 95% relative humidity the disease incidence was assessed.

In a second experiment, four field trials were conducted in rice crop on farmers conditions. Applications with the selected compounds (see Table 31 b) was conducted 2 times with an interval of 7 days in between applications. Water volume used was 200 L/ha. Crops were showing the first sign of disease occurrence at the first application timing and the infection of the crop was in natural conditions (no inoculation).

Results:

The results of foliar experiments of a mixture of Streptomyces chrestomyceticus CBS 149411 TGAI and chemical compounds are shown in Tables 10 to 38. Treatment of the mixtures is compared with the treatments by a composition of S. chrestomyceticus CBS 149411 alone and the chemical compound alone.

The chemical compound (2^nd product) used in the experiments of Tables 10 to 38 is indicated in the title of the Tables.

Table 10: Disease control by S. chrestomyceticus CBS 149411 mixed with Fenpropidin

Table 11 : Disease control by S. chrestomyceticus CBS 149411 mixed with Difenoconazole

Table 12: Disease control by S. chrestomyceticus CBS 149411 mixed with Benzovindiflupyr

Table 13: Disease control by S. chrestomyceticus CBS 149411 mixed with Azoxystrobin,

Table 14: Disease control by S. chrestomyceticus CBS 149411 mixed with Bromuconazole

Table 15: Disease control by S. chrestomyceticus CBS 149411 mixed with Chlorothalonil Table 16: Disease control by S. chrestomyceticus CBS 149411 mixed with Pydiflumetofen, CAS 1228284-64-7 (Emulsifiable concentrate) Table 17. Disease control by S. chrestomyceticus CBS 149411 mixed with Prothioconazole,

Table 18: Disease control by S. chrestomyceticus CBS 149411 mixed with Folpet Table 19: Disease control by S. chrestomyceticus CBS 149411 mixed with Pydiflumetofen (Miravis Bold) Table 20: Disease control by S. chrestomyceticus CBS 149411 mixed with Pyraclostrobin

Table 21 : Disease control by S. chrestomyceticus CBS 149411 mixed with Metyltetraprole

Table 22: Disease control by S. chrestomyceticus CBS 149411 mixed with Isoflucypram

Table 23: Disease control by S. chrestomyceticus CBS 149411 mixed with Proquinazid Table 24: Disease control by S. chrestomyceticus CBS 149411 mixed with Mefentrifluconazole

Table 25: Disease control by S. chrestomyceticus CBS 149411 mixed with Cyprodinil Table 26: Disease control by S. chrestomyceticus CBS 149411 mixed with Fenpicoxamid,

Table 27: Disease control by S. chrestomyceticus CBS 149411 mixed with acibenzolar-S-methyl,

Table 28: Disease control by S. chrestomyceticus CBS 149411 mixed with Spiroxamine

Table 29: Disease control by S. chrestomyceticus CBS 149411 mixed with Florylpicoxamid, Table 30: Disease control by S. chrestomyceticus CBS 149411 mixed with compound disclosed in WO 2017/153380, CAS :2132414-04-9, product: suspension concentrate

Table 31 : Disease control by S. chrestomyceticus CBS 149411 mixed with compound disclosed in WO 2017/153380, CAS :2132414-04-9, product: emulsion concentrate

Table 31 b: Disease control by S. chrestomyceticus CBS 149411 mixed with feneptamidoquin (defined as a composition comprising N-[(2RS)-2,4-dimethyl-l-phenylpentan-2-yl]-8-fluoroqiiinoline-3-carboxamide, containing 80-100% of the R-enantiomer, or N-[(lRS)-l,3-dimethyl-l-(phenylmethyl)butyl)]-8-fluoro-3- quinolinecarboxamide, containing 80-100% of the R-enantiomer). Table 32: Disease control by S. chrestomyceticus CBS 149411 mixed with zinc thiazole

Table 33: Disease control by S. chrestomyceticus CBS 149411 mixed with Fosetyl-aluminium,

Table 34: Disease control by S. chrestomyceticus CBS 149411 mixed with oxolinic acid,

Table 35: Disease control by S. chrestomyceticus CBS 149411 mixed with Anisiflupurin,

Table 36: Disease control by S. chrestomyceticus CBS 149411 mixed with Ipflufenoquin,

Table 37: Disease control by S. chrestomyceticus CBS 149411 mixed with Quinofumelin,

Table 38: Disease control by S. chrestomyceticus CBS 149411 mixed with Tricyclazole, Table 39: Disease control by S. chrestomyceticus CBS 149411 mixed with Fluxapyroxad,

Conclusions

The results in Tables 3 to 8 and 10 to 39 show that mixtures of a formulation based on Streptomyces sp. Saigon413 CBS149411 and several chemical products are effective against several fungal diseases.

The chemical mixing products cover a range of different groups and mode of action, including DMI fungicides, Amines, SDHI fungicides, AP-fungicides, Qol-fungicides, Azanaphthalenes, MBI-R, Qil- fungicides, carboxylic acids, DHODHI fungicides, dithiocarbamates, phthalimides, chloronitriles, Benzothiadiazole and phosphonates. The mixtures shown in Tables 10 to 39 show that these are a means for disease control or disease reduction in wheat and rice. All mixtures effective for the control of Puccinia recondita, Pyricularia oryzae or Zymoseptoria tritici provided at least 30% disease control, in at least one mixture ratio. The disease control level for a majority of the mixtures indicated a synergistic interaction of Streptomyces sp. isolate CBS149411 with the mixture partner. In particular, synergistic interaction is suggested for the mixtures with acibenzolar-S-methyl, azoxystrobin, benzovindiflupyr, bromuconazole, chlorothalonil, cyprodinil, difenoconazole, fenpicoxamid, fenpropidin, florylpicoxamid, folpet, isoflucypram, mefentrifluconazole, metyltetraprole, proquinazid, prothioconazole, pydiflumetofen, pyraclostrobin, spiroxamine and cyclobutri fluram. (Original in Electronic Form) (This sheet is not part of and does not count as a sheet of the international application)

FOR RECEIVING OFFICE USE ONLY

FOR INTERNATIONAL BUREAU USE ONLY

Claims

Claims

1. A composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces chrestomyceticus, and component (B) comprises a compound selected from the group consisting of prothioconazole, difenoconazole, mefentrifluconazole, hexaconazole, propiconazole, bromuconazole, fenpropidin, spiroxamine, isoprothiolane, pydiflumetofen, benzovindiflupyr, isoflucypram, isopyrazam, fluxapyroxad, cyprodinil, azoxystrobin, pyraclostrobin, metyltetraprole, proquinazid, tricyclazole, florylpicoxamid, fenpicoxamid, feneptamidoquin, N-(2,4- dimethyl-1-phenylpentan-2-yl)-8-fluoroquinoline-3-carboxamide, ipflufenoquin, quinofumelin, zinc thiazole, folpet, chlorothalonil, fosetyl-aluminium, oxolinic acid, anisiflupurin, inpyrfluxam, metalaxyl, metalaxyl-M, triticonazole, penthiopyrad, sedaxane, thiabendazole, imidacloprid, thiamethoxam, fludioxonil, fluoxastrobin, silthiofam, chlorantraniliprole, ethaboxam, ipconazole, cyantraniliprole, oxathiapiprolin, fluopyram, picarbutrazox, cyclobutrifluram, isocycloseram, and fluoxapiprolin.

2. The composition according to claim 1 , wherein the Streptomyces chrestomyceticus, comprises a nucleotide sequence which has at least 99.8 % identity to SEQ ID NO: 1 .

3. The composition according to claim 1 or 2, wherein the Streptomyces chrestomyceticus comprises a genome sequence which has at least 95% identity to the whole genome of Streptomyces sp. Saigon413 deposited with the Westerdijk Institute under accession number CBS149411.

4. The composition according to any one of the claims 1 to 3, wherein the component (A) comprises a cell count of the Streptomyces of 10° to 10¹⁴ cfu / g dry weight.

5. The composition according to any one of the claims 1 to 4, wherein component (A) further comprises a metabolite, preferably wherein the metabolite comprises malonomicin.

6. The composition according to claim 5, wherein component (A) comprises at least one further metabolite selected from the group consisting of cyclothiazomycin C, streptimidone, an oligosaccharide compound which comprises a molecular formula according to C53H90N2O44, further characterised by the NMR spectra listed in Table 1 and Table 2, and further characterized by a structural Formula I, a lipopeptide according to Formula II, or a salt thereof wherein R1 = CH3 or C2H5

Formula (II) and a polyene compound characterized by a molecular formula according to C67H115NO25, wherein the polyene is further characterized by the spectrum of light absorption as shown in Figure 10.

7. The composition according to any one of the claims 1 to 6, wherein component (B) comprises a compound selected from the group consisting of azoxy stro bin, benzovindiflupyr, bromuconazole, chlorothalonil, cyprodinil, difenoconazole, feneptamidoquin, fenpicoxamid fenpropidin, florylpicoxamid, folpet, isoflucypram, mefentrifluconazole, metyltetraprole, proquinazid, prothioconazole, pydiflumetofen, pyraclostrobin, and spiroxamine.

8. The composition according to any one of the claims 1 to 7, wherein the weight ratio of component (A) to component (B) is from 300: 1 to 1 : 100.

9. The composition according to any one of the claims 1 to 8, wherein the weight ratio of compound (A) to component (B) is from 200:1 to 1 : 50.

10. The composition according to any one of the claims 1 to 9, wherein component (B) comprises acibenzolar-S-methyl and wherein the weight ratio of component (A) to component (B) is from 150:1 to 1 :30.

11. The composition according to any one of the preceding claims, wherein the composition further comprises an agriculturally acceptable carrier and, optionally, a surfactant and/or formulation adjuvants.

12. A method of controlling or preventing phytopathogenic diseases, preferably phytopathogenic fungi, on a plant or on plant propagation material and / or on harvested food crops, which comprises applying to the plant, on plant propagation material, the locus thereof, and / or on harvested food crops the composition as defined in any one of claims 1 to 11 .

13. The method according to claim 12, wherein the phytopathogenic fungi belong to a genus belonging to Zymoseptoria, Puccinia, Pyricularia, Fusarium, Pythium, Monographella, Microdochium, Glomerella, Colletotrichum, and / or Botrytis, preferably the phytopathogenic fungi belong to a species belonging to Zymoseptoria tritici, Puccinia recondita f. sp. tritici, Pyricularia oryzae, Pythium ultimum, Fusarium culmorum, Monographella nivalis syn. Microdochium nivale, Fusarium nivale, Fusarium graminearum, Septoria tritici, Glomerella lagenarium syn. Colletotrichum lagenarium, and/ or Botrytis cinerea.

14. The method according to any one of the claims 11 to 13, wherein the plant comprises wheat, barley, rice, corn, soya, sugar beet banana, tomato, cucumber, and / or groundnut.

15. A plant, plant propagation material, the locus thereof and/or harvested food crops comprising a composition according to any one of the claims 1 to 11 .