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WO2025196169A3 - Criblage d'expression de protéine à haut débit - Google Patents

Criblage d'expression de protéine à haut débit

Info

Publication number
WO2025196169A3
WO2025196169A3 PCT/EP2025/057590 EP2025057590W WO2025196169A3 WO 2025196169 A3 WO2025196169 A3 WO 2025196169A3 EP 2025057590 W EP2025057590 W EP 2025057590W WO 2025196169 A3 WO2025196169 A3 WO 2025196169A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
cfps
reagents
protein expression
high throughput
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2025/057590
Other languages
English (en)
Other versions
WO2025196169A2 (fr
Inventor
Tobias William Barr Ost
Richard J. PAOLINI. JR.
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nuclera Ltd
Original Assignee
Nuclera Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB2404024.8A external-priority patent/GB202404024D0/en
Priority claimed from GBGB2404261.6A external-priority patent/GB202404261D0/en
Application filed by Nuclera Ltd filed Critical Nuclera Ltd
Publication of WO2025196169A2 publication Critical patent/WO2025196169A2/fr
Publication of WO2025196169A3 publication Critical patent/WO2025196169A3/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des procédés de synthèse de protéines acellulaires, des réactifs de synthèse de protéines acellulaires (CFPS) optimisés, et des procédés d'optimisation de réactifs CFPS pour augmenter les rendements d'expression de protéines par criblage à haut débit d'une sélection de compositions d'expression en parallèle à l'aide de faibles volumes de réactifs. Les procédés sont applicables à l'expression de protéines dans des tubes ou des plaques à l'aide d'une couche de fluide hydrophobe pour empêcher l'évaporation.
PCT/EP2025/057590 2024-03-20 2025-03-20 Criblage d'expression de protéine à haut débit Pending WO2025196169A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB2404024.8A GB202404024D0 (en) 2024-03-20 2024-03-20 High throughput protein expression screening
GB2404024.8 2024-03-20
GB2404261.6 2024-03-25
GBGB2404261.6A GB202404261D0 (en) 2024-03-25 2024-03-25 High throughput protein expression screening

Publications (2)

Publication Number Publication Date
WO2025196169A2 WO2025196169A2 (fr) 2025-09-25
WO2025196169A3 true WO2025196169A3 (fr) 2025-10-30

Family

ID=95066129

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2025/057590 Pending WO2025196169A2 (fr) 2024-03-20 2025-03-20 Criblage d'expression de protéine à haut débit

Country Status (1)

Country Link
WO (1) WO2025196169A2 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008112980A2 (fr) * 2007-03-15 2008-09-18 Incitor, Llc Procédé et système pour l'assemblage de macromolécules et de nanostructures
WO2023152518A1 (fr) * 2022-02-14 2023-08-17 Nuclera Ltd Procédés pour optimiser des réactifs de synthèse de protéines acellulaires
WO2024013487A1 (fr) * 2022-07-11 2024-01-18 Nuclera Ltd Protéines fluorescentes améliorées

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008112980A2 (fr) * 2007-03-15 2008-09-18 Incitor, Llc Procédé et système pour l'assemblage de macromolécules et de nanostructures
WO2023152518A1 (fr) * 2022-02-14 2023-08-17 Nuclera Ltd Procédés pour optimiser des réactifs de synthèse de protéines acellulaires
WO2024013487A1 (fr) * 2022-07-11 2024-01-18 Nuclera Ltd Protéines fluorescentes améliorées

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ALBERTI SIMON ET AL: "A User's Guide for Phase Separation Assays with Purified Proteins", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 430, no. 23, 23 June 2018 (2018-06-23), pages 4806 - 4820, XP085513322, ISSN: 0022-2836, DOI: 10.1016/J.JMB.2018.06.038 *
ANGENENDT P ET AL: "CELL-FREE PROTEIN EXPRESSION AND FUNCTIONAL ASSAY IN NANOWELL CHIP FORMAT", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 76, no. 7, 1 April 2004 (2004-04-01), pages 1844 - 1849, XP001196723, ISSN: 0003-2700, DOI: 10.1021/AC035114I *
PHAN CHI M. ET AL: "Can Water Float on Oil?", LANGMUIR, vol. 28, no. 10, 28 February 2012 (2012-02-28), US, pages 4609 - 4613, XP093216601, ISSN: 0743-7463, DOI: 10.1021/la204820a *
SUR KUNAL ET AL: "Immiscible Phase Nucleic Acid Purification Eliminates PCR Inhibitors with a Single Pass of Paramagnetic Particles through a Hydrophobic Liquid", THE JOURNAL OF MOLECULAR DIAGNOSTICS, AMERICAN SOCIETY FOR INVESTIGATIVE PATHOLOGY AND THE ASSOCIATION FOR MOLECULAR PATHOLOGY, vol. 12, no. 5, 1 September 2010 (2010-09-01), pages 620 - 628, XP009169239, ISSN: 1525-1578, DOI: 10.2353/JMOLDX.2010.090190 *

Also Published As

Publication number Publication date
WO2025196169A2 (fr) 2025-09-25

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