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WO2025017327A3 - Réactifs d'expression de protéines - Google Patents

Réactifs d'expression de protéines

Info

Publication number
WO2025017327A3
WO2025017327A3 PCT/GB2024/051915 GB2024051915W WO2025017327A3 WO 2025017327 A3 WO2025017327 A3 WO 2025017327A3 GB 2024051915 W GB2024051915 W GB 2024051915W WO 2025017327 A3 WO2025017327 A3 WO 2025017327A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein expression
methods
reagents
cfps
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/GB2024/051915
Other languages
English (en)
Other versions
WO2025017327A2 (fr
Inventor
Thomas A. GUILLIAM
Tobias William Barr Ost
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nuclera Ltd
Original Assignee
Nuclera Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB2311184.2A external-priority patent/GB202311184D0/en
Priority claimed from GBGB2311792.2A external-priority patent/GB202311792D0/en
Priority claimed from GBGB2317339.6A external-priority patent/GB202317339D0/en
Application filed by Nuclera Ltd filed Critical Nuclera Ltd
Publication of WO2025017327A2 publication Critical patent/WO2025017327A2/fr
Publication of WO2025017327A3 publication Critical patent/WO2025017327A3/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des procédés de synthèse de protéines acellulaires, des réactifs de synthèse de protéines acellulaires (CFPS) optimisés, et des procédés d'optimisation de réactifs CFPS pour augmenter les rendements d'expression de protéines à l'aide de NTP supplémentaires et/ou d'une source d'énergie supplémentaire ajoutés à un modèle d'acide nucléique. Les procédés sont applicables à l'expression de protéines sur un dispositif microfluidique ayant des surfaces hydrophobes par fusion de gouttelettes sur le dispositif afin de cribler une sélection de compositions d'expression en parallèle.
PCT/GB2024/051915 2023-07-20 2024-07-22 Réactifs d'expression de protéines Pending WO2025017327A2 (fr)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
GBGB2311184.2A GB202311184D0 (en) 2023-07-20 2023-07-20 Protein expression reagents
GB2311184.2 2023-07-20
GB2311792.2 2023-08-01
GBGB2311792.2A GB202311792D0 (en) 2023-08-01 2023-08-01 Protein expression reagents
GBGB2317339.6A GB202317339D0 (en) 2023-11-13 2023-11-13 Protein expression reagents
GB2317339.6 2023-11-13
GB202402250 2024-02-16
GB2402250.1 2024-02-16

Publications (2)

Publication Number Publication Date
WO2025017327A2 WO2025017327A2 (fr) 2025-01-23
WO2025017327A3 true WO2025017327A3 (fr) 2025-10-23

Family

ID=92212804

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2024/051915 Pending WO2025017327A2 (fr) 2023-07-20 2024-07-22 Réactifs d'expression de protéines

Country Status (1)

Country Link
WO (1) WO2025017327A2 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080153128A1 (en) * 2006-10-23 2008-06-26 Isu Abxis Co., Ltd. Cell-Free Protein Synthesis
JP2020127365A (ja) * 2017-04-28 2020-08-27 Ｓｐｉｂｅｒ株式会社 無細胞タンパク質合成用反応混合物、これを用いた無細胞タンパク質合成方法、及び無細胞タンパク質合成用キット
WO2023278593A1 (fr) * 2021-06-30 2023-01-05 Corteva Agriscience Llc Systèmes d'expression acellulaire à base de soja et de maïs
US20230092310A1 (en) * 2020-02-14 2023-03-23 Nuclera Nucleics Ltd. Methods for cell-free protein expression

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI691361B (zh) 2017-10-18 2020-04-21 美商電子墨水股份有限公司 包含具薄膜電晶體及電容感測之雙基板的數位微流體裝置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080153128A1 (en) * 2006-10-23 2008-06-26 Isu Abxis Co., Ltd. Cell-Free Protein Synthesis
JP2020127365A (ja) * 2017-04-28 2020-08-27 Ｓｐｉｂｅｒ株式会社 無細胞タンパク質合成用反応混合物、これを用いた無細胞タンパク質合成方法、及び無細胞タンパク質合成用キット
US20230092310A1 (en) * 2020-02-14 2023-03-23 Nuclera Nucleics Ltd. Methods for cell-free protein expression
WO2023278593A1 (fr) * 2021-06-30 2023-01-05 Corteva Agriscience Llc Systèmes d'expression acellulaire à base de soja et de maïs

Also Published As

Publication number Publication date
WO2025017327A2 (fr) 2025-01-23

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