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WO2024238989A3 - Recombinant terminal deoxynucleotidyl transferase enzyme - Google Patents

Recombinant terminal deoxynucleotidyl transferase enzyme Download PDF

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Publication number
WO2024238989A3
WO2024238989A3 PCT/US2024/030092 US2024030092W WO2024238989A3 WO 2024238989 A3 WO2024238989 A3 WO 2024238989A3 US 2024030092 W US2024030092 W US 2024030092W WO 2024238989 A3 WO2024238989 A3 WO 2024238989A3
Authority
WO
WIPO (PCT)
Prior art keywords
terminal deoxynucleotidyl
deoxynucleotidyl transferase
tdt
peptides
transferase enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/030092
Other languages
French (fr)
Other versions
WO2024238989A2 (en
Inventor
Javier Ignacio CÁCERES MOLINA
Andrea Magdalena CHANIQUE SALLUSTI
Jennifer Hsin-wen HSIEH
Cynthia Tamara SANHUEZA RIVERA
Daniela Julia CONTADOR ZANFORLÍN
Julián Esteban SÁEZ ARDURA
Tamara Lissette AKENTJEW FAURE
Javier Andrés GIMPEL HENRÍQUEZ
Marisol Andrea MORALES SOTO
Manuel Rozas Andreu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kura Biotech Inc
Original Assignee
Kura Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kura Biotech Inc filed Critical Kura Biotech Inc
Publication of WO2024238989A2 publication Critical patent/WO2024238989A2/en
Publication of WO2024238989A3 publication Critical patent/WO2024238989A3/en
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Provided herein are, inter alia, are terminal deoxynucleotidyl transferase (TdT) peptides. These TdT peptides are capable of synthesizing oligonucleotides using nucleoside triphosphate or 3'-O-blocked nucleoside triphosphates with high fidelity and efficiency. Also provided herein are, inter alia, compositions, kits, and methods of using the TdT peptides provided herein including embodiments thereof. The compositions, kits, and methods provided herein are a novel and efficient alternative for the synthesis of oligonucleotides in various biotechnological applications.
PCT/US2024/030092 2023-05-17 2024-05-17 Recombinant terminal deoxynucleotidyl transferase enzyme Pending WO2024238989A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CL202301425 2023-05-17
CL2023001425 2023-05-17

Publications (2)

Publication Number Publication Date
WO2024238989A2 WO2024238989A2 (en) 2024-11-21
WO2024238989A3 true WO2024238989A3 (en) 2025-04-24

Family

ID=93519903

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/030092 Pending WO2024238989A2 (en) 2023-05-17 2024-05-17 Recombinant terminal deoxynucleotidyl transferase enzyme

Country Status (1)

Country Link
WO (1) WO2024238989A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190112627A1 (en) * 2016-06-24 2019-04-18 The Regents Of The University Of California Nucleic Acid Synthesis and Sequencing Using Tethered Nucleoside Triphosphates
US20190211315A1 (en) * 2018-01-08 2019-07-11 Dna Script Variants of Terminal Deoxynucleotidyl Transferase and Uses Thereof
US20220119781A1 (en) * 2019-02-04 2022-04-21 Nuclera Nucleics Ltd. Modified terminal deoxynucleotidyl transferase (tdt) enzymes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190112627A1 (en) * 2016-06-24 2019-04-18 The Regents Of The University Of California Nucleic Acid Synthesis and Sequencing Using Tethered Nucleoside Triphosphates
US20190211315A1 (en) * 2018-01-08 2019-07-11 Dna Script Variants of Terminal Deoxynucleotidyl Transferase and Uses Thereof
US20220119781A1 (en) * 2019-02-04 2022-04-21 Nuclera Nucleics Ltd. Modified terminal deoxynucleotidyl transferase (tdt) enzymes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE GenBank 26 February 2021 (2021-02-26), ANONYMOUS: "TDT nucleotidylexotransferase, partial [Amia calva] ", XP093308397, Database accession no. MBN3304422.1 *
DATABASE Protein 18 October 2024 (2024-10-18), ANONYMOUS: "DNA nucleotidylexotransferase isoform X1 [Lepisosteus oculatus] - Protein - NCBI", XP093308401, Database accession no. XP-015202194.1 *

Also Published As

Publication number Publication date
WO2024238989A2 (en) 2024-11-21

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