[go: up one dir, main page]

WO2024026287A3 - Synthesis of substoichiometric chemically modified mrnas by in vitro transcription - Google Patents

Synthesis of substoichiometric chemically modified mrnas by in vitro transcription Download PDF

Info

Publication number
WO2024026287A3
WO2024026287A3 PCT/US2023/070903 US2023070903W WO2024026287A3 WO 2024026287 A3 WO2024026287 A3 WO 2024026287A3 US 2023070903 W US2023070903 W US 2023070903W WO 2024026287 A3 WO2024026287 A3 WO 2024026287A3
Authority
WO
WIPO (PCT)
Prior art keywords
ψtp
chemically modified
triphosphate
substoichiometric
synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2023/070903
Other languages
French (fr)
Other versions
WO2024026287A2 (en
Inventor
Cynthia J. Burrows
Aaron M. Fleming
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Utah Research Foundation Inc
Original Assignee
University of Utah Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Utah Research Foundation Inc filed Critical University of Utah Research Foundation Inc
Publication of WO2024026287A2 publication Critical patent/WO2024026287A2/en
Publication of WO2024026287A3 publication Critical patent/WO2024026287A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Described herein are systems and methods of synthesizing a chemically modified RNA polynucleotide having a defined and stoichiometric amount of a modified uridine nucleotide analog. In some embodiments, the systems and methods may comprise modified uridine triphosphates selected from the group consisting of pseudouridine triphosphate (ΨTP), N1-methylpseudouridine triphosphate (m1 ΨTP), N1-ethylpseudouridine triphosphate (e1 ΨTP), and N1-propylpseudouridine triphosphate (p1 ΨTP). In some embodiments, the systems and methods may comprise a DNA-dependent RNA polymerase.
PCT/US2023/070903 2022-07-25 2023-07-25 Synthesis of substoichiometric chemically modified mrnas by in vitro transcription Ceased WO2024026287A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US202263391851P 2022-07-25 2022-07-25
US63/391,851 2022-07-25
US202263425958P 2022-11-16 2022-11-16
US63/425,958 2022-11-16
US202263476547P 2022-12-21 2022-12-21
US63/476,547 2022-12-21

Publications (2)

Publication Number Publication Date
WO2024026287A2 WO2024026287A2 (en) 2024-02-01
WO2024026287A3 true WO2024026287A3 (en) 2024-04-18

Family

ID=89707588

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/070903 Ceased WO2024026287A2 (en) 2022-07-25 2023-07-25 Synthesis of substoichiometric chemically modified mrnas by in vitro transcription

Country Status (1)

Country Link
WO (1) WO2024026287A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250305019A1 (en) * 2024-03-29 2025-10-02 New England Biolabs, Inc. Compositions, kits, and methods for in vitro transcription

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190309337A1 (en) * 2017-08-18 2019-10-10 Modernatx, Inc. Rna polymerase variants
WO2021214204A1 (en) * 2020-04-22 2021-10-28 BioNTech SE Rna constructs and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190309337A1 (en) * 2017-08-18 2019-10-10 Modernatx, Inc. Rna polymerase variants
WO2021214204A1 (en) * 2020-04-22 2021-10-28 BioNTech SE Rna constructs and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
AARON M FLEMING: "Nanopore sequencing for N1-methylpseudouridine in RNA reveals sequence-dependent discrimination of the modified nucleotide triphosphate during transcription", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 51, no. 4, 28 February 2023 (2023-02-28), GB , pages 1914 - 1926, XP093164031, ISSN: 0305-1048, DOI: 10.1093/nar/gkad044 *

Also Published As

Publication number Publication date
WO2024026287A2 (en) 2024-02-01

Similar Documents

Publication Publication Date Title
US7888018B2 (en) Methods and kits for nucleic acid amplification
US9752172B2 (en) Methods and kits for sense RNA synthesis
EP4464778A3 (en) Direct oligonucleotide synthesis on cdna
WO2009145828A3 (en) Two slow-step polymerase enzyme systems and methods
WO2020077227A3 (en) Enzymatic rna synthesis
WO2006081222A3 (en) Isothermal dna amplification
MX2024011205A (en) Polymerases, compositions, and methods of use
WO2022256440A3 (en) Gene editing systems comprising a crispr nuclease and uses thereof
US9441264B2 (en) Whole transcriptome sequencing
WO2024026287A3 (en) Synthesis of substoichiometric chemically modified mrnas by in vitro transcription
EP1917362A1 (en) Methods and kits for sense rna synthesis
EP4397772A3 (en) Homopolymer primers for amplification of polynucleotides created by enzymatic synthesis
WO2006061425A3 (en) Production of beta-lactam antibiotics by genetically modified non-naturally producing microorganisms
US8802867B2 (en) Method for producing a sense RNA molecule
WO2023245126A3 (en) Products and compositions
JP2012050393A (en) Nucleic acid isothermal amplification method
LT2017523A (en) N4-modified cytidine nucleotides and their use
CA2061443A1 (en) Potato plant producing essentially amylose-free starch
WO2024192269A3 (en) Enzymatic methods and compositions for polymerization of phosphoramidate and thiophosphoramidate-linked dna
WO2022115822A3 (en) Methods for full-length reverse transcription pcr of long rnas containing modified ribonucleosides using a thermostable reverse transcriptase
AU2004239283A1 (en) Methods for amplification of nucleic acid sequences using staggered ligation
WO2024097674A3 (en) Modified short interfering nucleic acid (sina) molecules and uses thereof
CY1109285T1 (en) RECOVERY OF DNA
WO2005095591A3 (en) Novel genes from a biosynthesis gene cluster for aminoglycoside antibiotic synthesis and the thus obtained novel aminoglycoside antibiotics
TW200508394A (en) Method of detecting micrometastasis

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 05/06/2025)