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WO2024236008A3 - Nouvelles estérases et leurs utilisations - Google Patents

Nouvelles estérases et leurs utilisations Download PDF

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Publication number
WO2024236008A3
WO2024236008A3 PCT/EP2024/063319 EP2024063319W WO2024236008A3 WO 2024236008 A3 WO2024236008 A3 WO 2024236008A3 EP 2024063319 W EP2024063319 W EP 2024063319W WO 2024236008 A3 WO2024236008 A3 WO 2024236008A3
Authority
WO
WIPO (PCT)
Prior art keywords
esterase
varianten
seq
polyethylene terephthalate
esterases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2024/063319
Other languages
English (en)
Other versions
WO2024236008A2 (fr
Inventor
Isabelle ANDRÉ
Grégory ARNAL
Nicolas Chabot
Marc GUEROULT
Alain Marty
Alexandra TAUZIN
Vincent TOURNIER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carbios SA
Original Assignee
Carbios SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carbios SA filed Critical Carbios SA
Publication of WO2024236008A2 publication Critical patent/WO2024236008A2/fr
Publication of WO2024236008A3 publication Critical patent/WO2024236008A3/fr
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Sustainable Development (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

La présente invention concerne de nouvelles estérases, plus particulièrement des variants d'estérase ayant une activité améliorée et/ou une thermostabilité améliorée dans des conditions basiques par rapport à l'estérase parente selon SEQ ID NO:1 ou SEQ ID NO:2 et leurs utilisations pour dégrader un matériau contenant du polyester, tel que des produits en plastique. Les estérases de l'invention sont particulièrement appropriées pour dégrader le polyéthylène téréphtalate, et un matériau contenant du polyéthylène téréphtalate.
PCT/EP2024/063319 2023-05-15 2024-05-15 Nouvelles estérases et leurs utilisations Pending WO2024236008A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP23305772.8 2023-05-15
EP23305772 2023-05-15

Publications (2)

Publication Number Publication Date
WO2024236008A2 WO2024236008A2 (fr) 2024-11-21
WO2024236008A3 true WO2024236008A3 (fr) 2024-12-26

Family

ID=86605254

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2024/063319 Pending WO2024236008A2 (fr) 2023-05-15 2024-05-15 Nouvelles estérases et leurs utilisations

Country Status (2)

Country Link
TW (1) TW202511481A (fr)
WO (1) WO2024236008A2 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018011281A1 (fr) * 2016-07-12 2018-01-18 Carbios Nouvelles estérases et utilisations associées
WO2020021116A1 (fr) * 2018-07-27 2020-01-30 Carbios Nouvelles estérases et leurs utilisations
WO2020021117A1 (fr) * 2018-07-27 2020-01-30 Carbios Nouvelles estérases et leurs utilisations
WO2023019222A1 (fr) * 2021-08-11 2023-02-16 Biometis Technology, Inc. Dégradation enzymatique de polyéthylène téréphtalate

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2984354A1 (fr) 2011-12-20 2013-06-21 Centre Nat Rech Scient Procede de preparation d'alliage polymere/enzymes
US10124512B2 (en) 2012-11-20 2018-11-13 Carbios Method for recycling plastic products
CA2947478C (fr) 2014-05-16 2022-09-13 Carbios Procede de recyclage d'articles de plastique de polyethylene terephtalate mixtes
US10723848B2 (en) 2015-06-12 2020-07-28 Carbios Masterbatch composition comprising a high concentration of biological entities
CA3024287C (fr) 2016-05-19 2024-06-11 Carbios Procede de degradation de produits plastiques
KR102649944B1 (ko) 2016-07-12 2024-03-22 까르비오 신규한 에스테라아제 및 그의 용도
CA3073791A1 (fr) 2017-08-31 2019-03-07 Carbiolice Article en polyester biodegradable comprenant des enzymes
MX2020002290A (es) 2017-08-31 2020-07-13 Carbios Composicion liquida que comprende entidades biologicas y usos de la misma.
US11851689B2 (en) 2018-07-27 2023-12-26 Carbios Esterases and uses thereof
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
FR3088069B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de production d'acide terephtalique a l'echelle industrielle
EP3838976B1 (fr) 2019-12-19 2025-02-12 Carbios Procédé de dégradation de produits plastiques
MX2022007496A (es) 2019-12-19 2022-07-01 Carbios Proceso para degradar productos plasticos.
CN117866280A (zh) 2019-12-19 2024-04-12 卡比奥斯公司 发泡塑料组合物

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018011281A1 (fr) * 2016-07-12 2018-01-18 Carbios Nouvelles estérases et utilisations associées
WO2020021116A1 (fr) * 2018-07-27 2020-01-30 Carbios Nouvelles estérases et leurs utilisations
WO2020021117A1 (fr) * 2018-07-27 2020-01-30 Carbios Nouvelles estérases et leurs utilisations
WO2023019222A1 (fr) * 2021-08-11 2023-02-16 Biometis Technology, Inc. Dégradation enzymatique de polyéthylène téréphtalate

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE Geneseq [online] 19 March 2020 (2020-03-19), "Metagenome-derived cutinase variant (V26).", retrieved from EBI accession no. GSP:BHG01941 Database accession no. BHG01941 *
URBANEK ANETA K ET AL: "Biochemical properties and biotechnological applications of microbial enzymes involved in the degradation of polyester-type plastics", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - PROTEINS & PROTEOMICS, ELSEVIER, NETHERLANDS, vol. 1868, no. 2, 16 November 2019 (2019-11-16), XP086038547, ISSN: 1570-9639, [retrieved on 20191116], DOI: 10.1016/J.BBAPAP.2019.140315 *

Also Published As

Publication number Publication date
WO2024236008A2 (fr) 2024-11-21
TW202511481A (zh) 2025-03-16

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