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WO2024220793A2 - Procédés de traitement de la moisissure - Google Patents

Procédés de traitement de la moisissure Download PDF

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Publication number
WO2024220793A2
WO2024220793A2 PCT/US2024/025386 US2024025386W WO2024220793A2 WO 2024220793 A2 WO2024220793 A2 WO 2024220793A2 US 2024025386 W US2024025386 W US 2024025386W WO 2024220793 A2 WO2024220793 A2 WO 2024220793A2
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WO
WIPO (PCT)
Prior art keywords
mold
space
sanitizing composition
concentration
triethylene glycol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/025386
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English (en)
Other versions
WO2024220793A3 (fr
Inventor
Emile Etienne GRIGNARD III
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Grignard Pure LLC
Original Assignee
Grignard Pure LLC
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Filing date
Publication date
Application filed by Grignard Pure LLC filed Critical Grignard Pure LLC
Publication of WO2024220793A2 publication Critical patent/WO2024220793A2/fr
Publication of WO2024220793A3 publication Critical patent/WO2024220793A3/fr
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/14—Disinfection, sterilisation or deodorisation of air using sprayed or atomised substances including air-liquid contact processes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2101/00—Chemical composition of materials used in disinfecting, sterilising or deodorising
    • A61L2101/32—Organic compounds
    • A61L2101/34—Hydroxy compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
    • A61L2209/20—Method-related aspects
    • A61L2209/21—Use of chemical compounds for treating air or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
    • A61L2209/20—Method-related aspects
    • A61L2209/22—Treatment by sorption, e.g. absorption, adsorption, chemisorption, scrubbing, wet cleaning

Definitions

  • TECHNICAL FIELD This disclosure relates to compositions and methods for treating molds or mold spores in a space (i.e., an outdoor or indoor space).
  • a space i.e., an outdoor or indoor space.
  • a mold is one of the structures certain fungi can form. The dust-like, colored appearance of molds is due to the formation of spores containing fungal secondary metabolites. The spores are the dispersal units of the fungi.
  • Mold spores are a common component of household and workplace dust. When mold spores are present in large quantities, they can present a health hazard to humans, potentially causing allergic reactions and respiratory problems. Some molds also produce mycotoxins that can pose serious health risks to humans and animals. Exposure to high levels of mycotoxins can lead to neurological problems and, in some cases, death. Prolonged exposure (e.g., daily home exposure) can be particularly harmful. Molds in the home can usually be found in damp, dark or steamy areas, such as bathrooms, kitchens, cluttered storage areas, recently flooded areas, basement areas, plumbing spaces, areas with poor ventilation. Molds can also be found outdoors in humid environments.
  • Molds caused by mold allergy are: watery, itchy eyes; a chronic cough; headaches or migraines; difficulty breathing; rashes; tiredness; sinus problems; nasal blockage; and frequent 1 Attorney’s Docket No.50908-0010WO1 sneezing. Molds can also pose a hazard to human and animal health when they are consumed following the growth of certain mold species in stored food. Thus, there is a need for improved methods and compositions for treating mold in spaces occupied by human and animals, such as homes, health care facilities, food packaging facilities, and other settings.
  • this disclosure features a method for reducing a concentration of mold spores in a space, the method including treating a space containing an active mold source with an effective amount of a sanitizing composition containing triethylene glycol, thereby reducing the concentration of the mold spores suspended in the air within the space by at least about 99%.
  • this disclosure features a method for reducing mold spore transmission, the method including contacting a mold (e.g., on a surface of an article) or a mold spore in a space in the presence of an active mold source with an effective amount of a sanitizing composition containing triethylene glycol, thereby reducing the mold spore transmission by at least about 80%.
  • this disclosure features a method for preventing mold spores from forming, the method including treating a space containing an active mold source with an effective amount of a sanitizing composition 2 Attorney’s Docket No.50908-0010WO1 containing triethylene glycol, thereby preventing generation of mold spores in the space.
  • the term “treat molds or mold spores,” “treating molds or mold spores”, or “treatment of molds or mold spores” can include inactivating molds or mold spores, reducing the amount or concentration of molds or mold spores (e.g., in the air or on a surface), reducing transmission of mold spores, reducing settling of mold spores, and/or preventing a mold from generating mold spores.
  • the term “inactivating molds or mold spores” refers to killing molds or mold spores or otherwise make the molds or mold spores inactive.
  • the term “active mold source” refers to a live mold capable of generating mold spores.
  • suitable spaces that can be treated by the compositions and methods described herein include those in homes, offices, schools, hotels, 3 Attorney’s Docket No.50908-0010WO1 lobbies, theaters, reception rooms, bathrooms, health care facilities (e.g., nursing homes, hospital rooms (e.g., intensive care facilities), and medical offices (e.g., dental offices)), food packaging facilities, institutional kitchens, cafeterias, restaurants, public transportation vehicles (buses, trains, subways, and airplanes), ambulances, indoor stadiums and athletic facilities, law enforcement facilities (e.g., prisons), government facilities, elevators, retail locations, and other indoor public or private spaces.
  • health care facilities e.g., nursing homes, hospital rooms (e.g., intensive care facilities), and medical offices (e.g., dental offices)
  • food packaging facilities e.g., institutional kitchens, cafeterias, restaurants, public transportation vehicles (
  • the sanitizing composition described herein can be used to treat mold or mold spores either in an unoccupied space (e.g., a space not occupied by human or animals) or an occupied space (e.g., a space occupied by human or animals).
  • an unoccupied space e.g., a space not occupied by human or animals
  • an occupied space e.g., a space occupied by human or animals
  • the molds (as well as spores produced from such molds) that can be treated by the sanitizing composition described herein can include Aremonium, Alternaria, Aspergillus, Botrytis, Cladosporium, Fusarium, Geotrichum, Manoscus, Monilia, Mortierella, Mucor, Neurospora, Oidium, Oosproa, Penicilium, Rhizopus, Stachybotrys, Thamnidium, Trichoderma, and Trichophyton.
  • this disclosure features a sanitizing composition containing (e.g., comprising, consisting essentially of, or consisting of) triethylene glycol and water (e.g., deionized water).
  • Triethylene glycol is miscible with water, has a boiling point of 286.5 ⁇ C at a pressure of 101.325 kPa, and has a relative low vapor pressure compared to water. Without wishing to be bound by theory, it is believed that triethylene glycol treats or inactivates a mold or mold spore by condensing on particles, droplets, or surfaces containing the mold or mold spore until the concentration of triethylene glycol becomes sufficiently high to denature the mold or mold spore. Further, without wishing to be bound by theory, it is believed that triethylene glycol is highly hygroscopic and can inactivate (e.g., kill) a mold or mold spore by absorbing water from the mold or mold spores.
  • triethylene glycol has very low acute or chronic toxicity when inhaled or ingested (especially at the level used in the air to treat (e.g., sanitize or disinfect) an indoor space) and therefore is safe to use in indoor spaces (occupied or unoccupied).
  • the amount of triethylene glycol in the sanitizing composition described herein is not particular limited and can vary as desired.
  • a sanitizing composition containing a relatively low amount of triethylene glycol can achieve the same disinfection effect against a mold or mold spore as a sanitizing composition containing a relatively high amount of triethylene glycol by applying the former composition in a space at a higher frequency or in a higher amount.
  • the sanitizing composition described herein can include triethylene glycol in an amount of from at least about 1% (e.g., at least about 5%, at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 52%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, or at least about 80%) by weight to at most about 99.5% (e.g., at most about 99%, at most about 95%, at most about 90%, at most about 85%, at most about 80%, at most about 75%, at most about 70%, at most about 65%, at most about 60%, or at most about 50%) by weight of the composition.
  • at least about 1% e.g., at least about 5%, at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 52%, at least about 55%, at least about 60%, at least about 65%, at least about 70%,
  • triethylene glycol can be 100% of the sanitizing composition described herein (i.e., without any other ingredient). It is believed that applying a sanitizing composition containing a relatively high amount (e.g., at least about 50% by weight) of triethylene glycol can increase the efficiency of the disinfection and reduce the frequency of the application of the composition.
  • the water in the sanitizing composition described herein is deionized water, reverse osmosis (RO) water, or ultrapure water (e.g., when used in a vaporizer).
  • the water can have a 5 Attorney’s Docket No.50908-0010WO1 resistivity of at least 17 mega Ohms, a total organic carbon content of at most about 10 ppb, a bacterial count of at most about 10 CFU/ml).
  • the water can include ions in an amount of from at most about 50 ppm (e.g., at most about 40 ppm, at most about 30 ppm, at most about 20 ppm, at most about 10 ppm, at most about 5 ppm, or at most about 1 ppm) to at least about 1 ppb (e.g., at least about 10 ppb) of the total amount of the water.
  • the water in the composition can be tap water (i.e., not deionized water, RO water, or ultrapure water).
  • the sanitizing composition described herein can include water in an amount of from at least about 1% (e.g., at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 48%, at least about 50%, at least about 60%, or at least about 70%) by weight to at most about 99% (e.g., at most about 95%, at most about 90%, at most about 85%, at most about 80%, at most about 75%, at most about 70%, at most about 65%, at most about 60%, at most about 50%, or at most about 48%) by weight of the composition.
  • deionized water, RO water, or ultrapure water can minimize clogging the nozzles (e.g., caused by deposition of minerals in water) of the system (e.g., a vaporizer) used to apply the sanitizing composition described herein and therefore can keep the system operating for an extended period of time.
  • the water in the sanitizing composition described herein can facilitate inactivation of molds or mold spores.
  • the composition can allow the composition to be readily nebulized, 6 Attorney’s Docket No.50908-0010WO1 atomized or vaporized (e.g., by reducing the vaporization temperature and/or increasing the evaporation rate when the sanitizing composition is applied by an atomizer such as a nebulizer, a humidifier, a fog/haze machine, or a smoke generator) and to form an aerosol or vapor in the atmosphere.
  • the water in the aerosol can evaporate rapidly to form fine TEG droplets, which have sanitizing effects and inactivate molds or mold spores in the air or on a surface.
  • the water in the sanitizing composition can render the composition inflammable, thereby resulting in a safer product than TEG alone (which is a flammable liquid having a flash point of 157 ⁇ C).
  • the sanitizing composition described herein can further include an optional ingredient, such as a glycol different from triethylene glycol.
  • the additional glycol can be propylene glycol. Without wishing to be bound by theory, it is believed that the additional glycol can either increase the sanitizing effect of the composition or increase the whiteness of the composition (e.g., to indicate that the sanitizing composition is present in the air).
  • the sanitizing composition described herein does not include any additional glycol or any components other than triethylene glycol and water.
  • the sanitizing composition described herein can include an additional glycol (e.g., propylene glycol (PG)) in an amount of from at least about 0.5% (e.g., at least about 1%, at least about 1.5%, at least about 2%, at least about 2.5%, at least about 3%, at least about 3.5%, at least about 4%, at least about 4.5%) by weight to at most about 99% (e.g., at most about 95%, at most about 90%, at most about 80%, at most about 70%, at most about 60%, at most about 50%, at most about 40%, at most about 30%, at most about 20%, at most about 10%, at most about 5%, at most about 4.5%, at most about 4%, at 7 Attorney’s Docket No.50908-0010WO1 most about 3.5%, at most about 3%, at most about 2.5%, at most
  • sanitizing composition described herein can optionally include a fragrance.
  • suitable fragrances can include essential oils, esters (e.g., lactones), alcohols, thiols, aldehydes, ketones, amines, hydrocarbons, and aromatic compounds.
  • the sanitizing composition described herein can include a fragrance in an amount of from at least about 0.1% (e.g., at least about 0.2%, at least about 0.3%, at least about 0.4%, or at least about 0.5%) by weight to at most about 1% (e.g., at most about 0.9%, at most about 0.8%, at most about 0.7%, at most about 0.6%, or at most about 0.5%) by weight of the composition.
  • the sanitizing composition described herein can optionally include a material generally recognized as safe (“GRAS”) as defined by the U.S. Food and Drug Administration.
  • GRAS a material generally recognized as safe
  • suitable GRAS materials include dimethyl ether, glycerin, chlorine dioxide, and hypochlorus acid.
  • the sanitizing composition described herein can include (e.g., comprise, consist essentially of, or consist of) from about 50% to about 90% by weight triethylene glycol and from about 10% to about 50% by weight water.
  • the sanitizing composition described herein can include (e.g., comprise, consist essentially of, or consist of) (1) triethylene glycol in an amount of from about 52% to about 90% by weight of the composition; (2) water in an amount of from about 5% to about 48% by weight of the composition; and (3) propylene glycol in an amount of from about 0% to about 5% (e.g., from about 0.5% to about 5%) by weight of the composition.
  • triethylene glycol in an amount of from about 52% to about 90% by weight of the composition
  • water in an amount of from about 5% to about 48% by weight of the composition
  • propylene glycol in an amount of from about 0% to about 5% (e.g., from about 0.5% to about 5%) by weight of the composition.
  • the sanitizing composition described herein can include (e.g., comprise, consist essentially of, or consist of) about 52.25% by weight triethylene glycol, about 1% by weight propylene glycol, and about 46.75% by weight water.
  • this disclosure also features a method of treating molds or mold spores in a space (i.e., an outdoor or indoor space).
  • the method can be a method for reducing a concentration of mold spores in a space.
  • such a method can include treating a space containing mold spores suspended in the air within the space by applying (e.g., dispersing, spraying, nebulizing, atomizing, or vaporizing) an effective amount of a sanitizing composition described herein into the space.
  • applying the sanitizing composition can be performed by a system that generates fog, smoke, or haze, such as a vaporizer (e.g., a smoke generator), a nebulizer (e.g., a scent dispersion unit), or an atomizer (e.g., a humidifier).
  • the system can be those known in the art, such as the fog/haze machines or smoke simulators used in emergency training or used in the lighting industry to generate theatrical effects.
  • the method can further include identifying a space in need of treatment of molds or mold spores (e.g., in need of reduction of the amount or concentration of molds or mold spores).
  • a space can be considered in need of treatment of molds or mold spores if it includes a flooded area, a damp area, a steamy area, or an area lacking ventilation.
  • a space can be considered in need treatment of molds or mold spores if its humidity is at least about 35% (e.g., at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, or at least about 80%) and/or at most about 100% (e.g., at most about 95% or at most about 90%).
  • the space to be treated can include an active mold source.
  • the sanitizing composition described herein can effectively treat molds or mold spores (e.g., reducing the concentration of mold spores, reducing mold spore transmission, or preventing mold spores from forming) for an extended period of time even in the presence of an active mold source (which can generate mold spores).
  • the method can further include vaporizing the sanitizing composition (e.g., in a humidifier, a fog/haze machine, or a smoke generator) before applying (e.g., spraying) the composition into a space.
  • vaporizing the composition can be performed by treating the composition with steam or heating.
  • the method can include delivering the composition to a heat exchanger to vaporize the composition.
  • the heated vapor can be forced through a nozzle as vapor and/or liquid droplets (or liquid particles) to form a visible or invisible aerosol, fog, smoke, or haze.
  • the sanitizing composition described herein when applied (e.g., dispersed or sprayed) into a space using a vaporizer, the vaporizer can have a liquid reservoir and can use an electric pump to propel the sanitizing composition in the liquid reservoir into a heat exchanger where the sanitizing composition is vaporized.
  • the heated vapor can be forced through a nozzle as vapor and liquid droplets (or liquid particles) that form an opaque fog, smoke, haze, or visible or non-visible aerosol.
  • an atomizer e.g., a nebulizer
  • the composition can be converted to an aerosol by pressure.
  • the composition can be pumped into a series of specific sized chambers in an 10 Attorney’s Docket No.50908-0010WO1 atomizer or nebulizer to increase the pressure and velocity of the composition in order to convert it from a liquid to an aerosol.
  • ultrasonic and/or vibrating mesh technology can be used to apply the composition into a space through an atomizer or a nebulizer.
  • applying the sanitizing composition can form vapor and/or liquid droplets (or liquid particles) that contain triethylene glycol.
  • the liquid droplets can form an aerosol that contains triethylene glycol.
  • applying the composition can form an aerosol, a vapor, or a mixture thereof.
  • the aerosol liquid droplets can have an average diameter of from at least about 10 nm (e.g., at least about 20 nm, at least about 50 nm, at least about 100 nm, at least about 200 nm, at least about 500 nm, at least about 1 ⁇ m, at least about 2 ⁇ m, or at least about 5 ⁇ m) to at most about 10 ⁇ m (e.g., at most about 8 ⁇ m, at most about 6 ⁇ m, at most about 5 ⁇ m, at most about 4 ⁇ m, at most about 2 ⁇ m, at most about 1 ⁇ m).
  • the method described herein can generate from at least about 2000 (e.g., at least about 3000, at least about 4000, at least about 5000, at least about 6000, at least about 8000, or at least about 10,000) to at most about 100,000 (e.g., at most about 50,000 or at most about 25,000) liquid droplets per cm 3 of the space (i.e., an outdoor or indoor space).
  • applying e.g., dispersing or spraying
  • the sanitizing composition can be performed intermittently (e.g., either at a constant interval or at irregular intervals).
  • the frequency of the application can vary as desired depending on factors such as the concentration of triethylene glycol in the composition, the temperature and humidity of the space, the size of the space, the desired concentration of the composition in the space, and the air exchange rates of the space.
  • the preferred temperature 11 Attorney’s Docket No.50908-0010WO1 of the space can range from about 5 ⁇ C to about 50 ⁇ C (e.g., from about 10 ⁇ C to about 30 ⁇ C or from about 15 ⁇ C to about 30 ⁇ C).
  • the preferred relative humidity of the space can range from about 5% to about 75% (e.g., from about 15% to about 70%, from about 30% to about 65%, or from about 45% to about 60%).
  • the time period between two applications of the composition can be from at least about 10 seconds (e.g., at least about 30 seconds, at least about 1 minute, at least about 1.5 minutes, at least about 2 minutes, at least about 5 minutes, at least about 10 minutes, at least about 30 minutes, or at least about 1 hour) to at most about 2 hours (e.g., at most about 1 hour, at most about 30 minutes, at most about 10 minutes, or at most about 5 minutes).
  • the concertation of the sanitizing composition in an aerosol form e.g., the concentration of the liquid droplets containing the sanitizing composition
  • the concertation of the sanitizing composition in an aerosol form can be from about 0.02 mg/m 3 to about 1.6 mg/m 3 (e.g., from about 0.04 mg/m 3 to about 1 mg/m 3 ).
  • the total concentration of the TEG (including TEG in the aerosol and TEG in the vapor) or glycol (e.g., all glycols in the aerosol and in the vapor) in a space can be from at least about 0.01 mg/m 3 (e.g., at least about 0.02 mg/m 3 , at least about 0.04 mg/m 3 , at least about 0.05 mg/m 3 , at least about 0.1 mg/m 3 , at least about 0.2 mg/m 3 , at least about 0.3 mg/m 3 , at least about 0.4 mg/m 3 , at least about 0.5 mg/m 3 , at least about 0.6 mg/m 3 , at least about 0.8 mg/m 3 , at least about 1 mg/m 3 , at least about 1.5 mg/m 3 , at least about 2 mg/m 3 , at least about 2.5 mg/m 3 , at least about 3 mg/m 3 , at least about 3.5
  • the total concertation of the TEG (including TEG in the aerosol and TEG in the vapor) or glycol (including all glycols in the aerosol and in the vapor) in a space can be from about 0.01 mg/m 3 to about 3 mg/m 3 (e.g., e.g., from about 0.5 mg/m 3 to about 3 mg/m 3 or from about 0.5 mg/m 3 to about 1 mg/m 3 ).
  • the method includes maintaining the concentration of the sanitizing composition in an aerosol form (e.g., in the form of aerosol particles) or the concentration of TEG within the above ranges for a period of time from at least about 30 minutes (e.g., at least about 1 hour, at least about 2 hours, at least about 4 hours, at least about 5 hours, at least about 6 hours, at least about 9 hours, or at least about 12 hours) to at most about 24 hours (e.g., at most about 21 hours, at most about 18 hours, at most about 15 hours, at most about 12 hours, at most about 9 hours, or at most about 6 hours) per day.
  • at least about 30 minutes e.g., at least about 1 hour, at least about 2 hours, at least about 4 hours, at least about 5 hours, at least about 6 hours, at least about 9 hours, or at least about 12 hours
  • at most about 24 hours e.g., at most about 21 hours, at most about 18 hours, at most about 15 hours, at most about 12 hours, at most about 9 hours
  • the method includes treating an indoor space with the 13 Attorney’s Docket No.50908-0010WO1 sanitizing composition described herein for at least two days (e.g., at least three days, at least four days, at least five days, at least six days, at least eight days, at least ten days, at least 15 days, at least 20 days, at least 30 days, at least one month, at least two months, at least three months, or at least six months) or continuously until the mold is believed to be effectively treated or treatment is no longer needed.
  • the 13 Attorney’s Docket No.50908-0010WO1 sanitizing composition described herein for at least two days (e.g., at least three days, at least four days, at least five days, at least six days, at least eight days, at least ten days, at least 15 days, at least 20 days, at least 30 days, at least one month, at least two months, at least three months, or at least six months) or continuously until the mold is believed to be effectively treated or treatment is no longer
  • the treatment days can be consecutive, in a certain frequency (e.g., every other day), or random within a period of time (e.g., a week, a month, three months, or a year).
  • a period of time e.g., a week, a month, three months, or a year.
  • maintaining the sanitizing composition or the TEG at a concentration within the above ranges for a relatively short period of time e.g., about 6 hours
  • the concentration reduction of mold spores refers to their gross concentration reduction, which includes both the concentration reduction by natural die-off of the mold spores and the concentration reduction of the mold spores caused by the sanitizing composition described herein. Without wishing to be bound by theory, it is believed that the concentration reduction of mold spores in the air by natural settling in an indoor space containing a viable mold may not reach 96% net reduction if the space is not sanitized as the mold can continue to generate mold spores.
  • the airborne mold spore concentration will be reduced by at least 96% for a relatively long period of time.
  • At least about 0.1 gram e.g., at least about 0.5 gram, at least about 1 gram, at least about 2 grams, at least about 3 grams, or at least about 4 grams
  • at most about 5 grams of the sanitizing composition can be used 14 Attorney’s Docket No.50908-0010WO1 in a space having a volume of 1000 cubic feet every 6 hours (e.g., every 4 hours, every 2 hours, or every 1 hour).
  • this disclosure features a method for reducing mold spore transmission.
  • the method can include contacting a mold (e.g., a mold on a surface of an article in the space) or a mold spore (e.g., a mold spore in the air of the space) with an effective amount of a sanitizing composition containing triethylene glycol described herein, thereby reducing the mold spore transmission.
  • a mold e.g., a mold on a surface of an article in the space
  • a mold spore e.g., a mold spore in the air of the space
  • the method can reduce mold transmission by at least about 80% (e.g., at least about 85%, at least about 90%, at least about 95%, at least about 98%, at least about 99%, at least about 99.5, or at least about 99.9%) or about 100%.
  • the method for reducing mold spore transmission can be performed under conditions (e.g., treatment time, aerosol concentration, aerosol particle size, and TEG concentration) or in a manner similar to those described above with respect to the method for reducing a concentration of mold spores.
  • the above method can reduce mold spore transmission by inactivating the mold within the space (which would prevent the mold from producing mold spores) or by inactivating the mold spores in the air of the space directly.
  • the method can reduce settling of mold spores on a surface by at least about 80% (e.g., at least about 85%, at least about 90%, at least about 95%, at least about 98%, at least about 99%, at least about 99.5, or at least about 99.9%) or about 100%.
  • the surface can be any surface of an article in the space exposed to the sanitizing composition described herein.
  • the surface can be a porous surface or a non-porous surface (e.g., a hard surface).
  • this disclosure features a method for preventing mold spore from forming.
  • the method can include treating a space with an effective amount of a sanitizing composition containing triethylene glycol described herein, thereby preventing generation of mold spores in the space.
  • the space includes a mold.
  • the method can prevent the existing mold from producing mold spores.
  • the space does not include a mold.
  • the method can prevent a viable mold or mold spore from being transmitted into the space.
  • the method for preventing mold spore from forming can be performed under conditions (e.g., treatment time, aerosol concentration, aerosol particle size, and TEG concentration) or in a manner similar to those described above with respect to the method for reducing a concentration of mold spores.
  • conditions e.g., treatment time, aerosol concentration, aerosol particle size, and TEG concentration
  • the above method can prevent mold spore from forming by inactivating the mold within the space (which would prevent the mold from producing mold spores).
  • a system described herein e.g., a vaporizer or an atomizer such as a nebulizer
  • multiple systems can be used at appropriate places to ensure even distribution of the sanitizing composition.
  • the sanitizing composition described herein can be applied from the system(s) into the indoor space until a desired sanitization (or disinfection) level is achieved.
  • the space (e.g., the indoor space) to be treated can include a mold spore suspending in the air and the sanitizing method described herein is capable of inactivating (e.g., killing) the mold spore in the air.
  • the space can include a mold or mold spore on a surface (e.g., either a hard or soft surface, or either a non-porous or a porous surface) and the sanitizing method described herein is capable of inactivating (e.g., killing) the mold or mold spore on the surface.
  • the surface can be any surface in an indoor space, such as a surface of a wall, a floor, a desk, a chair, a computer, a rug, or a drape.
  • a surface of a wall such as a surface of a wall, a floor, a desk, a chair, a computer, a rug, or a drape.
  • triethylene glycol can adhere to the mold or mold spore either in the air or on a surface to inactivate the mold or mold spore by dessication
  • this disclosure features a packaged product that includes a container (e.g., a can or a bottle), and the sanitizing composition described herein in the container.
  • the packaged product can be either pressurized or non-pressurized.
  • the following examples are illustrative and not intended to be limiting.
  • the bioaerosol reduction testing was conducted by aerosolizing mold off of an active mold source and sampling the chamber air to characterize the mold concentration in the chamber. This sampling occurred before and after each daily treatment.
  • the mold deposition testing was conducted by exposing glass slides to the active mold for 24 hours at a time to evaluate the effect of the treatment of Sanitizing Compound #1 on the amount of mold deposited. Six glass slides were placed in the test chamber and one slide was removed each day during the 6-day testing period. These tests were performed twice under the same conditions as Trial 1 and Trial 2. Chamber Preparation Prior to the initiation of testing, and between each trial, the chamber was wiped clean with 95% Isopropyl alcohol using a non-fibrous towel.
  • the purified bulk powder was retrieved from the freezer prior to each trial.
  • the spore stock was diluted to 1x10 4 cfu/mL in PBS.
  • Using this diluted spore stock six 19 Attorney’s Docket No.50908-0010WO1 (6) tryptic soy agar plates were coated with 750 ⁇ l of spore solution. The 750 ⁇ l was spread evenly across the entire plate before being allowed to dry. After drying, the 6 spore plates were placed into a sealable bag and moved into the incubator at 30°C. The mold plates were allowed to grow for 5 days before use in the test trial. After 5 days, the bag of plates was moved from the incubator into the 1 m 3 chamber.
  • the control was run to account for any natural die off of the organism over time in the chamber.
  • blank glass slides were placed in the chamber to assess the amount of settling yielded by the microorganism.
  • 1” x 2” glass slides were placed on the opposite side of the chamber from the mold plates and 2 ft. from the Aura diffuser.
  • To prepare the Aura diffuser for testing with the Sanitizing Composition #1 solution the reservoir of the Aura diffuser was filled with the Sanitizing Composition #1 solution.
  • the Aura diffuser was adjusted to settings determined in the concentration matching section of the protocol. Before being placed into the chamber, the Aura diffuser was primed. Priming the Aura diffuser consisted of running it outside of the chamber for 20 minutes.
  • the chamber air was sampled for 10 minutes.
  • the Aura diffuser was turned on.
  • the 21 Attorney’s Docket No.50908-0010WO1 Aura diffuser was set to maintain the concentration of Sanitizing Composition #1 in the chamber at 0.1 mg/m 3 throughout each treatment.
  • Each day the Aura diffuser was set to treat the chamber for 6 hours on a timed schedule. After the treatment, the sampling procedure was repeated to produce a post treatment concentration. Aerosol Collection Samples were collected using AGI-30 impingers or viable cascade bio impactors.
  • the AGI-30 impingers were filled with 20 mL of sterile PBS with the addition of 0.005% Tween 80.
  • Tween 80 was used to increase the impinger collection efficiency and for the de-agglomeration of all microorganisms. Sampling was done from opposite corners of the chamber simultaneously. After sampling, the impingers were pooled and collected in conical tubes for plating. The impinger sampling rate was 12.5 l/min using suction from the vacuum pumps. Each impinger used in the study was flow characterized prior to the initiation of the study. When using the viable cascade bio impactor, an agar plate was loaded into the impactor prior to beginning sampling. After sampling, the plates were covered and moved directly into the incubator. Aerosol samples were collected before and after treatment with Sanitizing Composition #1 each day.
  • Sample Plating For sample plating, 1.5 mL micro centrifuge tubes were filled with 900 ⁇ L of sterile PBS for dilution of the samples. The number of tubes needed was based on the number of samples to be plated and the predicted dilution ranges needed to plate the samples. Using the set of dilution tubes, serial dilutions were performed with the test samples. For this study, a standard small drop plaque assay technique was instituted for plating. The countable range for this assay was 5-50 plaques.
  • Counts >50 cfu were labeled as too numerous to count (TNTC).
  • the samples were plated in their respective dilution ranges in triplicate on pre-labeled culture plates. The plates were allowed to dry, then placed in the incubator set at 20-25°C for 48 hours.
  • 23 Attorney’s Docket No.50908-0010WO1 Incubation and Observation The plates were allowed to incubated at 20-25°C for 48 hours before being removed for enumeration. Plate counts were recorded in lab notebooks at the time of enumeration and transferred to Microsoft Excel spreadsheets for analysis. Study Controls Control Count: The A. brasiliensis nebulization stock yielded acceptable starting concentration counts of >1.0 x 10 7 cfu/ml.
  • Sterility Control The media sterility check revealed no growth of a contaminant.
  • Culture Purity The host culture showed no signs of contamination when being streak plated on a culture plate.
  • Neutralization Control Neutralizer yielded no signs of contaminant growth after being plated. Data Analysis Calculation of % Reduction and Log10 Reduction The starting bioaerosol concentrations of the trials were characterized in the first T-0 sample before any treatment with Sanitizing Composition #1 had taken place. To determine the log reduction of the trial at each time point, the percent of viable mold airborne in the chamber after the aerosolization period was compared to that initial T-0 bioaerosol concentration.
  • V p is viable particles in cfu per liter of air as calculated above. 25 Attorney’s Docket No.50908-0010WO1
  • C I C Imp ⁇ vol a ⁇ t Q imp •
  • the aerosol efficiency (expressed as %) is: E fficiency ⁇ C a ⁇ 100 V All runs were and standard deviation for each trial. Total viable biological concentrations along with reductions over time (i.e., log reductions of aerosolized biologicals) were calculated and graphed for each trial.
  • Test Results The results of bioaerosol reduction testing in the two trials mentioned above are summarized in Tables 1 and 2 below.
  • Sanitizing Composition #1 unexpectedly exhibited a net Log10 reduction of at least 2.64 after 102 hours against the spores of Aspergillus brasiliensis in the aerosol. In other words, the composition was able to kill more than 99.7% of the spores of Aspergillus brasiliensis in the aerosol after 102 hours.
  • the above results suggest that Sanitizing Composition #1 would be effective in killing mold spores in the aerosol form in a space in the presence of an active mold source.
  • the mold deposition test showed that the daily log10 reductions of Aspergillus brasiliensis settling on a glass slide ranged from 1.30 to 1.93 in Trial 1 and ranged from 1.00 to 1.93 in Trial 2 compared to a control experiment (where Sanitizing Composition #1 was not introduced into the test chamber).
  • the results showed that Sanitizing Composition #1 was able to reduce the settling of the spores of Aspergillus brasiliensis onto a glass slide by at least 90% each day.
  • the above results suggest that Sanitizing Composition #1 would be effective in preventing and reducing mold spore transmission and in preventing generation of mold spores from an active mold source.
  • Other embodiments are in the following claims. 28

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Abstract

La présente invention concerne un procédé de réduction d'une concentration de spores de moisissure dans un espace. Le procédé comprend le traitement d'un espace contenant des spores de moisissure en suspension dans l'air à l'intérieur de l'espace avec une quantité efficace d'une composition désinfectante contenant du triéthylène glycol, ce qui permet de réduire la concentration des spores de moisissure en suspension dans l'air dans cet espace.
PCT/US2024/025386 2023-04-20 2024-04-19 Procédés de traitement de la moisissure Pending WO2024220793A2 (fr)

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