WO2024215161A1 - Cosmetic composition containing zn-porphyrin and lignin for blocking ultraviolet rays - Google Patents

Abstract

The present invention relates to a cosmetic composition containing Zn-porphyrin and lignin for blocking ultraviolet rays. The cosmetic composition for blocking ultraviolet rays, according to the present invention, can effectively block UV-A and UV-B, which are harmful to the human body and, compared to when Zn-porphyrin or lignin alone is used, has significantly increased ultraviolet blocking efficiency.

Description

Cosmetic composition for sun protection containing zinc-porphyrin and lignin

The present invention relates to a cosmetic composition for blocking ultraviolet rays comprising zinc-porphyrin and lignin.

Ultraviolet rays are light rays with a wavelength of less than 400 nm emitted from the sun, and are divided into long-wave ultraviolet rays (UV-A; 320-400 nm), medium-wave ultraviolet rays (UV-B; 280-320 nm), and short-wave ultraviolet rays (UV-C; 200-280 nm). They cause photobiological reactions in the skin, leading to sunburn, pigmentation, photoaging, skin cancer, etc., and cause various skin damages depending on the wavelength.

Short-wave ultraviolet rays (UV-C) cause sterilization and cell death, but most of them are absorbed by the ozone layer and have difficulty reaching the skin, and medium-wave ultraviolet rays (UV-B) reach the basal layer of the epidermis and the upper part of the dermis, causing persistent skin keratinization, sunburn such as erythema and blisters, and pigmentation, and inducing cell membrane damage and skin cancer. In particular, long-wave ultraviolet rays (UV-A) reach the dermis, causing immediate pigmentation and delayed erythema, phototoxicity, photoallergic reactions, chronic photoaging due to free radical generation, and denaturing elastic fibers and collagen fibers in the dermis, reducing skin elasticity and causing wrinkles and skin atrophy. Therefore, the use of sunscreen is essential to protect the skin from UV rays.

UV blockers currently used in the cosmetics industry can be largely divided into inorganic UV blockers and organic UV blockers. In general, organic UV blockers refer to substances that absorb ultraviolet rays and exhibit a blocking effect by trapping solar energy within molecules, while inorganic UV blockers refer to substances that have physical properties that reflect and disperse ultraviolet rays and act only on the surface without being absorbed into the skin. Organic UV blockers have better spreadability than inorganic UV blockers, but their UV blocking effect is minimal, so inorganic UV blockers are generally preferred.

However, inorganic UV blockers such as titanium dioxide can cause discomfort due to a sticky feeling when applied to the skin, and can also make the skin look white or cloudy and shiny due to a whitish appearance. Recently, a technology to control inorganic UV blockers into nano-sized particles has been in the spotlight to enhance the UV blocking effect. However, in this case, there is a high possibility that excessive inorganic UV blockers dispersed into nano-sized particles can penetrate the skin pores, which can cause problems with skin health and safety, and there is a disadvantage that photoactivation caused by free radicals can cause skin irritation and damage.

Accordingly, the inventors of the present invention attempted to develop a sunscreen having excellent ultraviolet absorption effects, and confirmed that the ultraviolet absorption effect over a wide spectrum was enhanced when Zn-porphyrin produced from a recombinant microorganism and lignin derived from biomass were mixed, thereby completing the present invention.

Ultraviolet rays (UV) are classified into UV-A (320–400 nm), UV-B (280–320 nm), and UVC (100–280 nm) depending on the wavelength range. Among these, UV-A is hardly absorbed by the ozone layer and mostly reaches the Earth's surface. If exposed for a long time, it can affect the skin's immune system and cause skin aging or skin damage.

UV-B is mostly absorbed by the ozone layer and only a small portion reaches the surface of the earth, but it burns the skin and sometimes penetrates the skin tissue and can even cause skin cancer in severe cases. And UV-C is completely absorbed by the ozone layer in the stratosphere. Therefore, cosmetic compositions for UV protection must effectively block UV-A and UV-B, that is, ultraviolet rays with a wavelength range of 280 to 400 nm.

Accordingly, the purpose of the present invention is to provide a cosmetic composition for blocking ultraviolet rays having an excellent ultraviolet ray blocking effect.

In addition, another object of the present invention is to provide a method for protecting skin from damage caused by ultraviolet rays, comprising the step of topically applying the composition to an object in an effective amount.

To achieve the above purpose, the present invention provides a cosmetic composition for blocking ultraviolet rays containing zinc-porphyrin and lignin as effective ingredients.

The present invention also provides a method for protecting skin from damage caused by ultraviolet rays, comprising the step of topically applying the composition to a subject in an effective amount.

The cosmetic composition for blocking ultraviolet rays according to the present invention can effectively block UV-A and UV-B rays harmful to the human body, and has the effect of significantly increasing ultraviolet ray blocking efficiency compared to the case where zinc-porphyrin or lignin is used alone.

FIG. 1 is a diagram showing the results of evaluating the UV blocking performance of a Zn-PP cream according to one embodiment of the present invention (a: results of checking the absorbance of the Zn-PP cream, b: results of checking the transmittance of the Zn-PP cream, c: results of checking the rhodamine B photodecomposition of the Zn-PP cream, d: results of checking the hydroxyl radical scavenging activity of the Zn-PP cream).

FIG. 2 is a diagram showing the results of evaluating the UV blocking performance of a cream containing Zn-PP and/or lignin according to one embodiment of the present invention (a: results of confirming absorbance of a cream containing Zn-PP and/or lignin, b: results of confirming transmittance of a cream containing Zn-PP and/or lignin).

Hereinafter, the present invention will be described in detail with reference to the attached drawings as implementation examples of the present invention. However, the following implementation examples are presented as examples of the present invention, and if it is judged that a detailed description of a technology or configuration well known to those skilled in the art may unnecessarily obscure the gist of the present invention, the detailed description thereof may be omitted, and the present invention is not limited thereby. The present invention is capable of various modifications and applications within the scope of the following claims and equivalents interpreted therefrom.

In addition, the terms used in this specification are terms used to appropriately express preferred embodiments of the present invention, and may vary depending on the intention of the user or operator, or the customs of the field to which the present invention belongs. Therefore, the definitions of these terms should be made based on the contents throughout this specification. Throughout the specification, when a part is said to “include” a certain component, this does not mean that other components are excluded, but rather that other components can be further included, unless specifically stated otherwise.

Throughout this specification, '%' used to indicate the concentration of a particular substance, unless otherwise stated, is (w/w) % for solid/solid, (w/v) % for solid/liquid, and (v/v) % for liquid/liquid.

Hereinafter, the present invention will be described in more detail.

In one aspect, the present invention relates to a cosmetic composition for blocking ultraviolet rays, comprising zinc-porphyrin and lignin as active ingredients.

In one embodiment of the present invention, the zinc-porphyrin may be included at a concentration of 1 to 12% (w/v), preferably at a concentration of 3 to 10% (w/v), and more preferably at a concentration of 9 to 10% (w/v), but is not limited thereto.

In one embodiment of the present invention, the zinc-porphyrin may be produced from recombinant Corynebacterium glutamicum in which the heme biosynthesis pathway is engineered to produce zinc-porphyrin, and preferably, Corynebacterium glutamicum in which HemA, HemL, AlaS, DtxR, HemE and HrtBA genes are overexpressed, but is not limited thereto.

The term “Zn-porphyrin” as used in the present invention means Zn-coproporphyrin III or Zn-uroporphyrin III. The molecular formula of Zn-coproporphyrin III is C ₃₆ H ₃₈ N ₄ O ₈ Zn ⁺² , the IUPAC name is zinc;3-[8,13,18-tris(2-carboxyethyl)-3,7,12,17-tetramethyl-21,24-dihydroporphy-rin-2-yl]propanoic acid, and it has a molecular weight of 720.1 g/mol. The molecular formula of Zn-uroporphyrin III is C ₄₀ H ₃₈ N ₄ O ₁₆ Zn ⁺² , the IUPAC name is zinc;3-[7,12,18-tris(2-carboxyethyl)-3,8,13,17-tetrakis(carboxymethyl)-21,24-dihydroporphyrin-2-yl]propanoic acid, and it has a molecular mass of 896.1 g/mol.

In one embodiment of the present invention, the zinc-porphyrin may be represented by chemical formula 1 or chemical formula 2.

In one embodiment of the present invention, the lignin may be included at a concentration of 1 to 12% (w/v), preferably at a concentration of 3 to 10% (w/v), and more preferably at a concentration of 9 to 10% (w/v), but is not limited thereto.

The above lignin may be at least one selected from the group consisting of native lignin, lignosulfonate, dealkaline lignin, alkaline lignin, organosolv lignin, hydrolysis lignin, kraft lignin, soda lignin, steam explosion lignin, and dilute acid lignin, but is not limited thereto.

The term “lignosulfonate” used in the present invention, also called sulfite lignin, refers to lignin produced in the sulfite pulping process.

The term “dealkaline lignin” as used in the present invention means lignin produced from lignosulfonate produced from coniferous and deciduous trees through sodium sulfite treatment, through chemical modification such as partial desulfonation, oxidation, hydrolysis and demethylation.

The term “alkaline lignin” used in the present invention refers to the dealkalized lignin having its pH value adjusted to an alkaline range (pH8.0-10.0), and having higher water solubility than dealkalized lignin.

The term “organosolv lignin” used in the present invention refers to lignin produced through a chemical pretreatment method in the bioethanol production process. Representative methods for extracting organic solvent lignin include a method of extraction using an alcohol organic solvent process and a method of extraction using an acid organic solvent process.

The “cosmetic composition” of the present invention can be manufactured by including a cosmetically effective amount of the zinc-porphyrin and lignin of the present invention described above and a cosmetically acceptable carrier.

In this specification, “cosmetically effective amount” means an amount sufficient to achieve the UV protection efficacy of the composition of the present invention described above.

The external form of the cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. It may be provided in any formulation suitable for topical application, for example in the form of a solution, a gel, a solid, a pasty anhydrous product, an emulsion obtained by dispersing an oil phase in an aqueous phase, a suspension, a microemulsion, a microcapsule, a microgranule or a vesicular dispersion of ionic (liposomes) and nonionic type, or in the form of a cream, a skin, a lotion, a powder, an ointment, a spray or a concealer stick. These compositions can be prepared according to methods conventional in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to one embodiment of the present invention is not particularly limited in its formulation, and may be formulated as cosmetics such as, for example, a softening toner, an astringent toner, a nourishing toner, a nourishing cream, a massage cream, an essence, an eye cream, an eye essence, a cleansing cream, a cleansing foam, a cleansing water, a pack, a powder, a body lotion, a body cream, a body oil, and a body essence.

When the cosmetic composition of the present invention is in the form of a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component.

When the cosmetic composition of the present invention is in the form of a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and particularly in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether may be additionally included.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc. can be used as carrier components.

When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing, an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, methyl taurate, a sarcosinate, a fatty acid amide ether sulfate, an alkyl amidobetaine, an aliphatic alcohol, a fatty acid glyceride, a fatty acid diethanolamide, a vegetable oil, a linolenic derivative, or an ethoxylated glycerol fatty acid ester may be used as a carrier component.

The cosmetic composition of the present invention can be applied to cosmetics such as skin, lotion, cream, essence, pack, foundation, color cosmetics, sunscreen, two-way cake, face powder, compact, makeup base, skin cover, eye shadow, lipstick, lip gloss, lip fix, eyebrow pencil, toner, and cleansing agents such as shampoo and soap.

A cosmetic composition according to one embodiment of the present invention may further include functional additives and components included in general cosmetic compositions in addition to the zinc-porphyrin and lignin. The functional additives may include components selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular peptides, high molecular polysaccharides, sphingolipids, and seaweed extracts.

In addition to the functional additives, the cosmetic composition of the present invention may also contain ingredients included in general cosmetic compositions, if necessary. In addition, the contained ingredients may include oil components, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation promoters, cooling agents, antiperspirants, purified water, etc.

In one aspect, the present invention provides a method for protecting skin from damage caused by ultraviolet radiation, comprising the step of topically applying the composition to a subject in an effective amount.

Hereinafter, embodiments of the present invention will be described in more detail with reference to the attached drawings. However, the following embodiments are only intended to concretize the contents of the present invention, and the present invention is not limited thereby.

<Example 1> Production of Zn-porphyrin

1-1. Culture conditions of strains

Culturing of E. coli DH5α for replication was performed using LB medium at 37°C and 200 rpm. Corynebacterium glutamicum ATCC 14067 was pre-cultured in 20 ml of BHISG medium (medium containing 37 g BHI, 91 g sorbitol, and 2 g glucose per L) to secure the cell number. After pre-culture for 12-16 h at 30°C and 200 rpm, the culture was centrifuged at 4,000 rpm for 10 min to separate the cells, and the separated cells were pre-cultured once more in 200 ml of BHISG medium to secure the cell number for 2 L of the main culture. Flask cultures were performed in CGXY10 medium (42 g/L 3-morpholinopropanesulfonic acid (MOPS), 40 g/L glucose, 20 g/L ( _NH4 ) _{2SO4 ,} 5 g/L urea, 10 g/L _yeast extract, 1 g/L _KH2PO4 , 1 g/L _K2HPO4 , 250 mg/L _MgSO4 · _7H2O , 10 mg/L FeSO4·7H2O, 10 _mg /L _CaCl2 , 1 _mg /L ZnSO4· _7H2O , 0.31 mg/ _{L CuSO4} · _5H2O , 200 μg/L biotin, 100 μg/L MnSO4 _{· H2O} , and 20 μg/L NiCl2· 6H ₂ O) was used at 30°C, 200 rpm for 24 or 48 h. Fed-batch fermentation was performed as follows. All wild-type and recombinant Corynebacterium stored at cryogenic temperature were streaked on BHISG agar plates and cultured at 30°C for 24 h. One colony was then inoculated into 20 mL of BHISG medium and pre-cultured at 30°C, 200 rpm for 16 h. The first pre-cultured cell was inoculated into a 1000 mL shake flask containing 200 mL of BHISG medium and pre-cultured at 30°C, 200 rpm for 16 h. The second pre-cultured cells were inoculated into a 6-L fermentor containing CGXY10 medium excluding 2-L 3-morpholinopropanesulfonic acid (MOPS) at an optical density at 600 nm (OD 2). Fed-batch fermentation was performed in a 6-L fermentor at 30°C, 250–600 rpm, and pH was maintained at 7.0. Isopropyl β-d-1-thiogalactopyranoside (1 mM) and ethambutol at a concentration of 25 mg/L were added after 0 h of flask culture and 6 h of fed-batch fermentation. For E. coli culture, 25 mg/L kanamycin and 50 mg/L chloramphenicol were added to the LB medium, and for C. glutamicum culture, 25 mg/L kanamycin and 10 mg/L chloramphenicol were supplemented to the BHISG medium and CGXY10 medium. The cell culture of Corynebacterium glutamicum ATCC 14067 was centrifuged at 4,000 rpm for 10 minutes to separate the cells, and the supernatant containing zinc porphyrin was collected for the purification of zinc-porphyrin.

1-2. Purification of Zn-porphyrin

Zinc-porphyrin (Zn-PPs) was purified using preparative HPLC from Agela Technologies. Zinc porphyrin in the supernatant was purified using HP-20 Column (1500 g + 220 g) as the stationary phase and distilled water and 100% methanol as the mobile phase. The purification was performed at a flow rate of 100 ml/min for 220 minutes, and 75% purity zinc porphyrin was obtained under these conditions.

<Example 2> UV blocking performance test

To test the UV protection performance, 100 μL of purified Zn-PPs solutions containing 20, 40, and 80 mg/mL were mixed with 100 mg of base cream (Nivea, Germany) to prepare creams containing 1, 2, and 4% (w/v) of Zn-PPs, respectively.

UV protection performance test was conducted by applying each cream evenly to a glass slide (Marienfeld-SupeRior, Germany) attached with a transfer tape (3M corporation, Germany). After drying the sample in a dark room for 30 minutes, UV absorbance and transmittance were measured using a Cary Series UV-VIS-NIR spectrophotometer 5000 (Agilent Technologies, USA) at 1 nm intervals in the wavelength range of 290–400 nm, and the in vitro SPF value was calculated using the following formula:

E _λ : CIE red spectral effect

S _λ : solar spectral irradiance

T _λ : spectral transmittance of the sample

The critical wavelength (λc) is the wavelength at which the absorbance integral curve first reaches a ratio (R) greater than 0.9, and R was calculated using the following formula:

The UV-A/UV-B ratio was calculated using the following formula:

A _λ : absorbance value for each wavelength

The UV blocking properties were determined by evaluating the degree of decomposition of rhodamine B under UV irradiation. The residual rhodamine B (%) was calculated using the following equation:

A _o : Absorbance of the initial solution at 554 nm

A _t : Absorbance of the solution after exposure at 554 nm

Hydroxyl radical scavenging activity was measured using a modified 2-deoxyribose oxidation method.

First, 10 mM EDTA solution, 10 mM FeSO ₄ -7H ₂ O, 10 mM 2-deoxyribose, 10 mM hydrogen peroxide, 100 μL of 100 mM phosphate buffer (pH 7.4), and 20 μL of Zn-PPs sample were mixed and incubated at 37°C for 4 h. Then, 100 μL of 1% trichloroacetic acid and 2.8% thiobarbituric acid solution was injected and boiled for 10 min. After cooling on ice, the absorbance was measured at 520 nm. The hydroxyl radical scavenging activity was calculated using the following equation:

A _s : Absorbance of the sample at 520 nm

A _o : Absorbance of blank at 520 nm

A _c : Absorbance of the control group at 520 nm

The absorbance and transmittance of creams containing 1% (w/v), 2% (w/v), and 4% (w/v) of zinc porphyrin were determined, as shown in Fig. 1, showing that the higher the concentration of zinc porphyrin, the more UV rays were absorbed (Fig. 1a). In particular, the Zn-PP cream with a concentration of 4% (w/v) showed a transmittance of less than 50%, especially in the UV-A region (Fig. 1b), and the SPF value was 3.28 ± 0.68, which was 3.22 times higher than that of the base cream (Table 1). In addition, the critical wavelength of the Zn-PPS cream is 392.0 nm, which fully satisfies the requirements of a broad-spectrum UV blocker. The Zn-PP cream showed greater absorption in the UV-A region with a UV-A/UV-B ratio of 1.17 ± 0.04. As evidence, the Zn-PP cream also showed excellent ability to prevent photodegradation of rhodamine B under UV light (Fig. 1c). The Zn-PP cream at a concentration of 4% (w/v) was found to have the highest photoprotective effect among the samples with more than 60% of rhodamine B remaining. In addition, all concentrations of the Zn-PP cream showed hydroxyl radical scavenging activity, and the hydroxyl radical scavenging activity increased as the concentration increased (Fig. 1d).

Cream Nivea 1% Zn-PPs 2% Zn-PPs 4% Zn-PPs In vitro SPF 1.02 ± 0.03 1.33 ± 0.11 1.56 ± 0.25 3.28 ± 0.68 Critical wavelength (nm) - 393.0 ± 0.0 392.0 ± 0.0 392.0 ± 0.0 UV-A/UV-B Ratio - 1.25 ± 0.01 1.27 ± 0.01 1.17 ± 0.04

Next, to confirm the synergistic effect of zinc-porphyrin and lignin on broad-spectrum protection, 8% (w/v) alkaline lignin (AL, Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), 8% (w/v) dealkalizable lignin (DL, Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), and 8% (w/v) organosolv lignin (OL, Sugaren, Yongin, Korea) were mixed into 8% (w/v) Zn-PPs cream, respectively, to prepare ALZP, DLZP, and OLZP creams, and their UV protection performances were tested by the above method.

As a result, as shown in Fig. 2 and Table 2, the SPF values of the AL, DL, and OL creams at a concentration of 4% (w/v) containing only lignin were 2.04±0.13, 2.48±0.18, and 2.79±0.77, respectively, which were lower than that of the Zn-PP cream. The critical wavelengths were estimated to be 379.67±0.58, 382.0±1.0, and 386.33±2.08, respectively, indicating that they were broad-spectrum UV blockers. In addition, the AL, DL, and OL creams had relatively lower UV-A/UV-B ratios of 0.46±0.02, 0.49±0.02, and 0.71±0.01, respectively, compared to the Zn-PP cream, and showed higher absorption and lower transmittance in the UV-B region than in the UV-A region (Fig. 6). On the other hand, ALZP, DLZP, and OLZP creams all showed complementarity in the UV-B region (Fig. 2a), and the lowest transmittance was observed (Fig. 2b). In particular, compared to creams containing only Zn-PP or lignin, the SPF values of ALZP, DLZP, and OLZP all increased by more than 1.5 times, and among them, OLZP cream showed the highest SPF value. The critical wavelengths of ALZP, DLZP, and OLZP were similar to that of the Zn-PPs cream, and the UV-A/UV-B ratios of ALZP, DLZP, and OLZP creams were 0.93±0.02, 0.89±0.02, and 0.88±0.04, which were 2.02, 1.81, and 1.24 times higher than those of AL, DL, and OL creams, respectively.

Cream Nivea 4% Zn-
PPs 4% AL 4% DL 4% OL ALZP DLZP OLZP In vitro SPF 1.02 ± 0.03 3.28 ± 0.68 2.04 ± 0.13 2.48 ± 0.18 2.79 ± 0.77 5.04 ± 0.4 5.16 ± 0.67 6.21 ± 0.69 Critical wavelength (nm) - 392 ± 0.0 379.67 ± 0.58 382 ± 1.0 386.33 ± 2.08 390 ± 0 390.33 ± 0.58 390.67 ± 0.58 UV-A/UV-B Ratio - 1.17 ± 0.04 0.46 ± 0.02 0.49 ± 0.02 0.71 ± 0.01 0.93 ± 0.02 0.89 ± 0.02 0.88 ± 0.04

These results show that when zinc-porphyrin is mixed with lignin to manufacture a sunscreen, it exhibits superior UV blocking performance compared to sunscreens containing either alone, and in particular, among combinations of zinc-porphyrin and various types of lignin, it was confirmed that the OLZP cream UV blocking performance, which is a mixture of zinc-porphyrin and organosolv lignin, is the best.

As described above, specific embodiments of the present invention have been described in detail; however, those skilled in the art who understand the spirit of the present invention will be able to easily suggest other backward inventions or other embodiments included within the scope of the spirit of the present invention by adding, changing, deleting, etc. other components within the scope of the same spirit. Therefore, it should be understood that the embodiments described above are exemplary and not restrictive in all respects. The scope of the present invention is indicated by the scope of the claims described below rather than the detailed description described above, and all changes or modifications derived from the meaning and scope of the claims and the equivalent concept should be interpreted as being included in the scope of the present invention.

Claims (6)

A cosmetic composition for blocking ultraviolet rays, comprising zinc porphyrin and lignin as active ingredients. In the first paragraph, A cosmetic composition for blocking ultraviolet rays, characterized in that the zinc porphyrin is contained in a concentration of 1 to 12% (w/v). In the first paragraph, A cosmetic composition for blocking ultraviolet rays, characterized in that the lignin is contained in a concentration of 1 to 12% (w/v) In the first paragraph, A cosmetic composition for blocking ultraviolet rays, characterized in that the lignin is at least one selected from the group consisting of native lignin, lignosulfonate, dealkaline lignin, alkaline lignin, organosolv lignin, hydrolysis lignin, kraft lignin, soda lignin, steam explosion lignin, and dilute acid lignin. In the first paragraph, The composition is a cosmetic composition for blocking ultraviolet rays, characterized in that the composition has a formulation selected from the group consisting of skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser. A method for protecting skin from damage caused by ultraviolet rays, comprising the step of topically applying the composition of claim 1 to an object in an effective amount.

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