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WO2024209200A1 - Fibres creuses - Google Patents

Fibres creuses Download PDF

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Publication number
WO2024209200A1
WO2024209200A1 PCT/GB2024/050902 GB2024050902W WO2024209200A1 WO 2024209200 A1 WO2024209200 A1 WO 2024209200A1 GB 2024050902 W GB2024050902 W GB 2024050902W WO 2024209200 A1 WO2024209200 A1 WO 2024209200A1
Authority
WO
WIPO (PCT)
Prior art keywords
hollow fibres
acid
seq
composition
crosslinked
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/GB2024/050902
Other languages
English (en)
Inventor
Risto John MARTIN
Haydn Rhys INGRAM
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kalvotech Ltd
Original Assignee
Kalvotech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB2305209.5A external-priority patent/GB202305209D0/en
Priority claimed from GBGB2305210.3A external-priority patent/GB202305210D0/en
Priority claimed from GBGB2317377.6A external-priority patent/GB202317377D0/en
Application filed by Kalvotech Ltd filed Critical Kalvotech Ltd
Priority to AU2024250517A priority Critical patent/AU2024250517A1/en
Publication of WO2024209200A1 publication Critical patent/WO2024209200A1/fr
Priority to US18/971,697 priority patent/US12454773B2/en
Priority to US18/971,684 priority patent/US20250129521A1/en
Priority to US18/971,711 priority patent/US20250122647A1/en
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/14—Vegetable proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/14—Vegetable proteins
    • A23J3/16—Vegetable proteins from soybean
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/22—Working-up of proteins for foodstuffs by texturising
    • A23J3/225—Texturised simulated foods with high protein content
    • A23J3/227—Meat-like textured foods
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/22—Working-up of proteins for foodstuffs by texturising
    • A23J3/26—Working-up of proteins for foodstuffs by texturising using extrusion or expansion
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/02—Hollow fibre modules
    • B01D63/033—Specific distribution of fibres within one potting or tube-sheet
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01D67/0002—Organic membrane manufacture
    • B01D67/0009—Organic membrane manufacture by phase separation, sol-gel transition, evaporation or solvent quenching
    • B01D67/0011—Casting solutions therefor
    • B01D67/00113—Pretreatment of the casting solutions, e.g. thermal treatment or ageing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01D67/0002—Organic membrane manufacture
    • B01D67/0009—Organic membrane manufacture by phase separation, sol-gel transition, evaporation or solvent quenching
    • B01D67/0016—Coagulation
    • B01D67/00165—Composition of the coagulation baths
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01D67/0088—Physical treatment with compounds, e.g. swelling, coating or impregnation
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01D69/02—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01D71/06—Organic material
    • B01D71/56—Polyamides, e.g. polyester-amides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/58—Other polymers having nitrogen in the main chain, with or without oxygen or carbon only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/10—Hollow fibers or tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/10—Hollow fibers or tubes
    • C12M25/12—Hollow fibers or tubes the culture medium flowing outside the fiber or tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D1/00—Treatment of filament-forming or like material
    • D01D1/02—Preparation of spinning solutions
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D10/00—Physical treatment of artificial filaments or the like during manufacture, i.e. during a continuous production process before the filaments have been collected
    • D01D10/02—Heat treatment
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/06—Wet spinning methods
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/24—Formation of filaments, threads, or the like with a hollow structure; Spinnerette packs therefor
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F1/00—General methods for the manufacture of artificial filaments or the like
    • D01F1/02—Addition of substances to the spinning solution or to the melt
    • D01F1/08—Addition of substances to the spinning solution or to the melt for forming hollow filaments
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F1/00—General methods for the manufacture of artificial filaments or the like
    • D01F1/02—Addition of substances to the spinning solution or to the melt
    • D01F1/10—Other agents for modifying properties
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F11/00—Chemical after-treatment of artificial filaments or the like during manufacture
    • D01F11/02—Chemical after-treatment of artificial filaments or the like during manufacture of cellulose, cellulose derivatives, or proteins
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F6/00—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof
    • D01F6/02—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof from homopolymers obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • D01F6/16—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof from homopolymers obtained by reactions only involving carbon-to-carbon unsaturated bonds from polymers of unsaturated carboxylic acids or unsaturated organic esters, e.g. polyacrylic esters, polyvinyl acetate
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/22—Working-up of proteins for foodstuffs by texturising
    • A23J3/28—Working-up of proteins for foodstuffs by texturising using coagulation from or in a bath, e.g. spun fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2323/00—Details relating to membrane preparation
    • B01D2323/08—Specific temperatures applied
    • B01D2323/081—Heating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
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    • B01D2323/15—Use of additives
    • B01D2323/218—Additive materials
    • B01D2323/2181—Inorganic additives
    • B01D2323/21817—Salts
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01D2323/218—Additive materials
    • B01D2323/2182—Organic additives
    • B01D2323/21826—Acids, e.g. acetic acid
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • C—CHEMISTRY; METALLURGY
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Definitions

  • the instant disclosure relates in part to hollow fibres, as well as processes for their production and uses.
  • extruded or spun, semi- permeable, porous hollow fibres comprising covalent ester, thioester and/or amide crosslinked polypeptides are provided as well as processes for their production.
  • the hollow fibres of this disclosure may be produced from protein, protein extracts, and/or protein isolates derived from plants, animals, bacteria, algae, archaea, and/or fungi, and in certain embodiments are intended to be suitable for human and/or animal ingestion.
  • the hollow fibres of this disclosure may be designed to be used in the production of cartridges that are compatible with existing and/or novel bioreactor platforms, for harbouring cell cultures in cultured meat production.
  • United States Patent Application Publication No. US 2013/0192459 discloses “hollow fibre membranes having a supporting layer and an inner (i.e. on the lumen-facing side of the supporting layer) or an outer separating layer and to a process for manufacturing such hollow fibre membranes.”
  • PCT Application No. WO2019158494A1 discloses “an edible fiber comprising a biopolymer and a plasticiser; wherein the weight ratio of biopolymer to plasticiser is about 1 :0.25 to about 1 :3; and wherein the fiber has a diameter of about 0.5 pm to about 1 mm.”
  • EP0077098A2 discloses “hollow chitosan fibres for use in ultrafiltration and dialytic processes in general and renal dialysis in particular, and the process for their preparation.”
  • WO 2016/007879A1 discloses “a bioprotein tube having an outer diameter of about 1 mm and an inner diameter of about 0.80 may be prepared by extruding a bioprotein precursor solution comprising 50 mg/ml of alginate and gelatin at a ratio of 3:1 through an orifice into a solution of about 5 mg/ml calcium chloride and exposing the tube to a wash solution within about 10 sec.”
  • WO 2018/011805A2 discloses “a system for culturing cells which can be used, in some embodiments of the present invention, for generating edible meat”. It also states, “the culturing is performed on an edible hollow fiber cartridge”. Furthermore, “the vegetable - derived matrix is from a cereal family, legume (Fabaceae) family or a pseudocereal family”. Further still, “the legume is soy or pea”.
  • WO 2022/038241A1 discloses “a closed, continuous, semi-continuous or batch culture system for cell growth and differentiation followed by tissue growth for the production of, for example, clean meat.”
  • WO 2009/035414A1 discloses “The present invention relates to a chitosan construct and a method of preparing the same.
  • the chitosan construct may have a higher mechanical strength.
  • the present invention relates to chitosan fibre or chitosan hollow fibres”. It also states, “in particular, the construct may have a sponge-like porous structure.
  • the porosity of the construct may be up to 80 %. For example, the porosity may be about 20-80 %”.
  • WO 2022/038240A2 discloses “methods of production of structured clean meat products produced with the hollow fibers, cartridges and bioreactors”.
  • WO 2023/021213A1 discloses “[a] method of manufacture of crosslinked, edible, porous hollow fibers and sheet membranes suitable for the manufacture of clean meat products, the hollow fibers and sheet membranes made therefrom and methods of use thereof.”
  • “[t]he innovation of the present invention is to use physical crosslinking, said physical crosslinking being generated via an energy source such as one or more of heat, gamma, e-beam, beta, x- ray, or UV.”
  • JP2022072917A discloses “a cultured meat complex comprising a hollow fiber membrane or a decomposition product or lysate thereof and a cultured meat containing a cell group of an animal existing along the hollow fiber membrane or the decomposition product or lysate thereof’.
  • a cultured meat complex obtained by using the hollow fiber membrane composed of collagen may be subjected to heat treatment to gelatinize the collagen”.
  • WO 2023/152492A1 discloses “a substrate assembly for culturing cells, wherein the substrate assembly comprises: a plurality of edible fibres, wherein each fibre has an internal channel running along its length”. Wherein, “[t]he plurality of fibres may comprise alginate”. In which, “calcium chloride acts as a cross-linking agent for alginate”.
  • WO 2023/152493A1 discloses “a plurality of edible fibres, wherein each fibre has an internal channel running along its length”.
  • calcium chloride acts as a cross-linking agent for alginate
  • [t]he freeze-drying step can also be used to adjust the porosity of the final fibre material”.
  • [t]he freeze-drying step has been found to be particularly useful when the fibres comprise alginate”.
  • WO 2024/038281 A1 discloses “a method of culturing muscles cells for a comestible product, the method seeding muscle cells onto the one or more porous hollow fibres”.
  • the instant disclosure is based, at least in part, on the realization that extruded or spun, semi- permeable, porous hollow fibres comprising polycarboxylic acid derived covalent ester, thioester and/or amide crosslinked polypeptides, could enable the production of an edible high-protein cultured meat food product.
  • hollow fibres as disclosed herein can in some embodiments constitute a predominantly protein substrate, which eliminates the need to subsequently separate the cells from the substrate or scaffold on which they were grown. Consequently, the need for an additional seeding step into a tertiary scaffold, as typically required with traditional cultivation methods, could be negated.
  • the separation of cells from the extra-capillary space of existing semi-permeable hollow fibre bioreactors is a difficult step, and often requires the use of multiple enzyme washes. This separation step might not be necessary if the hollow fibres are themselves edible and could be consumed.
  • the disclosure is further based, at least in part, on the realization that extruded or spun, semi- permeable, porous hollow fibres comprising covalent ester, thioester and/or amide crosslinked polypeptides, may be used in the production of cartridges and modules that are compatible with existing and novel bioreactor platforms.
  • the hollow fibres of this disclosure could enable the realisation of high-density cell culture and the reduction of costs associated with decreased growth factor use in hollow fibre bioreactors. Collectively, these benefits could significantly decrease the cost in the production of cultured meat.
  • extruded or spun, semi-permeable, porous hollow fibres comprising covalent ester, thioester and/or amide crosslinked polypeptides, of this disclosure are in some aspects and/or embodiments edible, non-cytotoxic and/or biocompatible, and so they can be ingested and digested as food by humans and/or animals. Additionally, the hollow fibres of some aspects and embodiments of the disclosure can be formed and treated so as to enhance the texture and/or taste of the edible product.
  • Bioreactor platforms comprising cartridges and/or modules containing the hollow fibres of this disclosure may in many embodiments be used for the production of an edible food product comprising a mixture of the cultured cells and the hollow fibres of the disclosure.
  • An edible food product produced in this way may in certain embodiments be ingested and digested as food by humans and/or animals, without the risk of the inclusion of inedible substrate contaminants.
  • ester, thioester and/or amide crosslinked polypeptide derived polymers in general, is that ester, thioester and/or amide crosslinks can be broken by hydrolysis under certain conditions.
  • Beta-sheets and beta-coil secondary structures in polypeptides may prevent the hydrolysis of ester, thioester and/or amide bonds.
  • induced beta-sheets and beta-coils can revert to amorphous structures in water or upon hydration under certain conditions.
  • beta-sheets may collectively refer to both beta-sheets and beta-coil secondary structures.
  • the disclosure is also based, at least in part, on the realization that polymers comprising ester, thioester and/or amide crosslinked polypeptides with induced beta-sheet secondary structures may recrystalize upon being annealed, thereby relaxing the internal stresses within their crystal structures, such that the beta-sheet conformation becomes the stable micro-structure; a process by which is herein referred to as ‘protein annealing’. Consequently, the beta-sheet secondary structure of the crosslinked polypeptide polymer may remain intact upon rehydration and resist hydrolysis of the constituent ester, thioester and/or amide crosslinks.
  • a polymer material which includes a regular semi-crystalline polymer that includes ester, thioester and/or amide crosslinked polypeptides with a beta-sheet secondary structure - which herein, is referred to as Prokitein.
  • a ‘semi-crystalline polymer’ refers to a polymer that comprises molecular chains arranged in both crystalline and amorphous structures. In the crystalline regions, protein chains may be ordered in repeating patterns. In the amorphous regions, protein chains may be randomly orientated.
  • a ‘regular semi-crystalline polymer’ refers to a semi-crystalline polymer that comprises uniformly distributed crystal grain sizes and shapes, and/or grains that are orientated consistently relative to each other, within the crystalline phase(s) of its molecular structure.
  • the disclosure is also based, at least in part, on the realization that the resistance to hydrolysis of Prokitein could facilitate its use as materials for a plethora of applications, including, for hollow fibre production for long-term cell culture, as necessary in the production of cultivated meat products.
  • the extruded or spun, semi-permeable, porous hollow fibres comprising covalent ester, thioester and/or amide crosslinked polypeptides, of this disclosure comprise, in some aspects and/or embodiments, Prokiteins.
  • hollow fibres of many embodiments of this disclosure may overcome this shortcoming by additionally providing a process by which hollow fibres with porous structures may be formed without the use of freeze-drying.
  • It is yet another object of the disclosure to provide a food product comprising a combination of cultured cells and extruded or spun, semi-permeable, porous hollow fibres comprising covalent ester, thioester and/or amide crosslinked polypeptides.
  • the disclosure provides an edible, semi-permeable, porous hollow fibre, comprising one more polycarboxylic acid derived covalent ester, thioester and/or amide bond crosslinked polypeptides, wherein:
  • the external diameter of the hollow fibre is 50 - 6600 pM
  • the wall thickness of the hollow fibre is 20 - 800 pM
  • the lumen diameter of hollow fibre is 20 - 5000 pM
  • An edible, semi-permeable, porous hollow fibre comprising one more polycarboxylic acid derived covalent ester, thioester and/or amide bond crosslinked polypeptides, wherein:
  • the wall thickness of the hollow fibre is 20 - 800 pm;
  • the porosity of the hollow fibre is 1- 95 %.
  • the disclosure also provides a semi-permeable, porous hollow fibre, comprising covalent ester, thioester and/or amide bond crosslinked polypeptides, wherein:
  • the wall thickness of the hollow fibre is 20 - 800 pM
  • the lumen diameter of hollow fibre is 20 - 5000 pM
  • the disclosure also provides a semi-permeable, porous hollow fibre, comprising covalent ester, thioester and/or amide bond crosslinked polypeptides, wherein:
  • the wall thickness of the hollow fibre is 20 - 800 pm;
  • the porosity of the hollow fibre is 1- 95 %.
  • the disclosure provides a process for the production of a plurality of semi- permeable, porous hollow fibres, comprising covalent ester, thioester and/or amide bond crosslinked polypeptides, the process comprising the steps: a. combining: i. a first composition comprising polypeptides, with ii. a second composition comprising a solvent and one or more denaturing agents and/or reducing agents, to produce a third composition; b. incubating the third composition under conditions sufficient to denature and/or reduce at least a fraction of the polypeptides, to produce a fourth composition; c.
  • treating the covalently-crosslinked polypeptides with one of more postproduction modification processes selected from the group consisting of: i. treating the covalently-crosslinked polypeptides with a solvent to remove void inclusion elements; and ii. drying the covalently-crosslinked polypeptides; to produce to produce Prokitein.
  • cultured meat ’cultivated meat’, ‘cellular agriculture’, ‘cell-based meat’, ‘lab-grown meat’, ‘clean meat’, and ‘cultivated animal cell food product’ refer to the production of meat by culturing animal cells in vitro and are used interchangeably herein.
  • cultured meat sustainability, public health and animal welfare.
  • Bioreactors also referred to as ‘fermenters’ or ‘cultivators’, are devices or vessels that provide an environment for the growth and cultivation of cells, microorganisms, or biological molecules. Bioreactors are commonly used in biotechnology, pharmaceuticals, and biochemical engineering for the production of biological products such as cells, vaccines, enzymes and antibodies.
  • CSTRs may be fitted with various control systems to control an array of environmental conditions, such as temperature, pH, agitation speed, and the concentrations of dissolved metabolites (e.g., oxygen, carbon dioxide, glucose, lactic acid, etc.).
  • CSTRs are fitted with an energy source and mixing system as a means to control the environmental conditions.
  • FBS foetal bovine serum
  • CSTRs used for culturing cells in suspension or on micro -carriers are typically capable of producing cells at a density of 10 5 - 10 6 cells/mL (2; 3).
  • Petrochemical polymeric semi-permeable hollow fibres have been used to produce bioreactors capable of achieving and sustaining cell densities that are up to 35 times higher than that achieved in traditional tissue culture techniques (4). Additionally, the concentration of growth factors required to differentiate stem cells into their terminally differentiated state has been demonstrated to be between 2 and 8 times lower in ceramic semi-permeable hollow fibre bioreactors than in traditional adherent methods (5; 6).
  • the workflow typically adopted for cultured meat production is: (i) stem cell isolation, (ii) cultivation in suspension to a desired cell concentration; and (iii) seeding into scaffold(s).
  • the de-watering of the cell slurry between steps (ii) and (iii) is both time and energy intensive. Additionally, the final seeding step is made difficult by the fact that the design of the scaffold greatly influences cell seeding efficiency and the mouthfeel of the final product (3).
  • Hollow fibres such as those produced from petrochemical polymers, ceramics and cellulose acetate, have been used a substrate for culturing cells.
  • Successful development and commercial use of such hollow fibres has faced challenges such as the difficult separation of cultured cells from the hollow fibre substrates post-cultivation.
  • Polypeptides solubilized with acid or alkali can be used to produce hollow fibres, but this process can disrupt the primary protein structure, resulting in shorter chain lengths, lower chain entanglement, and weaker materials when crosslinked.
  • edible materials, such as polypeptides, even when crosslinked, are water soluble, and can swell significantly in an aqueous solution. Swelling may be undesirable, especially in bioreactor applications, as it can significantly disrupt the fluid and nutrient transport properties of these materials.
  • compositions and methods provided herein attempt to address and overcome some or all of these challenges.
  • the disclosure provides processes for the production of a plurality of semi-permeable, porous hollow fibres, comprising covalent ester, thioester and/or amide crosslinked polypeptides.
  • Step (a) comprises combining:
  • the first composition may comprise one or more polypeptides.
  • polypeptide and “protein” are used interchangeably herein.
  • the polypeptides of the hollow fibres and/or methods of the disclosure are obtained or derived from plants, animals, bacteria, algae, archaea, and/or fungi.
  • the polypeptides of the hollow fibres and/or methods of the disclosure are derived from one or more extracts and/or an isolates obtained from plants, animals, bacteria, algae, archaea, and/or fungi.
  • the polypeptides are plant polypeptides, wherein the term ‘plant’ includes, but is not limited to, legumes, nuts, seeds, cereals and tubers. Examples of such plant sources include, but are not limited to:
  • legumes chickpeas, lentils, kidney beans, black beans, navy beans, lima beans, pinto beans, soybeans, mung beans, adzuki beans, fava beans, black-eyed peas, green peas, snow peas, sugar snap peas, and/or split peas;
  • nuts almonds, walnuts, pistachios, cashews, brazil nuts, hazelnuts, macadamia nuts, pecans, pine nuts, peanuts, chestnuts and/or coconuts;
  • cereals wheat, rice, corn/maize, oats, barley, rye, millet, sorghum, quinoa, buckwheat, spelt, triticale, amaranth, teff, farro, kamut, freekeh, emmer, and/or fonio;
  • tubers potatoes, sweet potatoes, yams, cassava, taro, jicama, Jerusalem artichoke, water chestnut, Chinese yam, malanga, arrowroot, ginger, turmeric, turnips and/or beets.
  • the polypeptides are derived from legumes which include, but are not limited to: anasazi beans, cannellini beans, cocoa beans, coffee beans, cranberry beans, edamame, fayot (flageolet) beans, french green bean, gigante beans, great northern beans, long beans, marrowfat peas, fava beans (faba beans, broad beans), and purple string beans.
  • legumes include, but are not limited to: anasazi beans, cannellini beans, cocoa beans, coffee beans, cranberry beans, edamame, fayot (flageolet) beans, french green bean, gigante beans, great northern beans, long beans, marrowfat peas, fava beans (faba beans, broad beans), and purple string beans.
  • the polypeptides are animal polypeptides, wherein the term ‘animal’ includes, but is not limited to, mammals, marsupials, birds, fish, cephalopods, crustaceans and insects. Examples of such animal sources include, but are not limited to:
  • mammals cattle, sheep, pig, horse, goat, deer, reindeer, bison, moose, elk, camel, wild boar, wildebeest, and/or guinea pig;
  • birds chicken, turkey, duck, goose, quail, pheasant, guineafowl, ostrich, emu, pigeon, partridge, grouse, snipe, and/or woodcock;
  • cephalopods squid, octopus, cuttlefish, and/or nautilus
  • crustaceans shrimp, crab, lobster, crawfish, prawn, krill, crayfish, mussel, oyster, clam, scallop, and/or cockle;
  • insects grasshopper, cricket, mealworm, beetle, ant, termite, cicada, caterpillar, silkworm, locust, waxworm, hornworm, bamboo worm, scorpion, and/or centipede.
  • bacteria examples include, but are not limited to, Escherichia coli., Bacillus subtilis, and Pseudomonas fluorescens.
  • the polypeptides are algae polypeptides, wherein the term “algae” includes, but is not limited to, Euglenophyta, Chrysophyta, Pyrrophyta, Chlorophyta, Rhodophyta, Paeophyta, and Xanthophyta.
  • algal sources of polypeptides include, but are not limited to: Euglena gracilis, Diatoms, Dinoflagellates pyrocystis, Netrium desmid, Chlamydomonas, Spirogyra, Volvox, Ulva, Chlorella, Chara, Corallina, Gelidium, Gracilaria, Laminaria, Fucus, Sargassum, and Vaucheria.
  • polypeptides are archaea polypeptides, wherein the term “archaea” includes, but is not limited to, Euryarchaeota, Crenarchaeota and Korarchaeota.
  • archaea sources of polypeptides include, but are not limited to: Thermoproteus neutrophillus, Thermoproteus uzoniensis, Vulcanisaeta distributa, Vulcanisaeta moutnovskia, Metallosphaera cuprina, Metallosphaera sedula, Staphylothermus hellenicus, Staphylothermus marinus, Thermosphaera aggregans, Sulfolobus acidocaldarius, Sulfolobus islandicus, Desulfurococcus kamchatkensis, Hyperthermus butylicus, Thermus aqaticus, Archaeoglobus fulgidus, and Archaeoglobus veneficus
  • the polypeptides are fungi polypeptides, wherein the term “fungi” includes, but is not limited to, Ascomycota, Basidiomycota, Zygomycota, Chytridiomycota, Glomeromycota, and Deuteromycota.
  • fungi sources of polypeptides include, but are not limited to mushrooms, truffle, yeast, penicillium, aspergillus, ergot, chanterelle, morel, bracket fungi, coral fungus, stinkhorn, puffball, bird’s nest fungi, and jelly fungi.
  • Other examples include Saccharomyces cerevisiae and Pichia pastoris.
  • An extract or isolate may, for example, be an aqueous extract from a plant, animal, bacteria, algae, archaea, or fungi. This may be obtained, for example, by dissolving all or part of the organic source in an aqueous buffer (e.g., phosphate-buffered saline (PBS)), acid or alkali solution, optionally together with a surfactant, optionally with suitable mixing and/or homogenisation; and then isolating the aqueous extract or isolate from the undissolved material.
  • PBS phosphate-buffered saline
  • Solid protein may be recovered from the isolated sample through precipitation by adjusting the pH of the aqueous protein solution to the isoelectric point of the extracted protein through the addition of acid or alkali.
  • the solid protein precipitate may then be recovered through centrifugation, and subsequently washed with water and heated to sterilise the protein extract. Finally, the extracted protein may be spray dried (9).
  • the extract or isolate from a plant, animal, bacteria, algae, archaea, or fungi is a protein extract or isolate.
  • Protein extracts can be obtained, inter alia, during the production of defatted beans, seeds or nut flakes. Suitable plant materials first undergo a process of cleaning, drying, conditioning, cracking, dehulling, solvent oil extraction and flash solvent removal, and the resulting proteins are then purified via alcohol washing, acid leaching and/or water leaching (9).
  • bacteria, algae, archaea, or fungi may be genetically-modified to produce or excrete recombinant proteins.
  • the recombinant protein may be purified through precipitation of the protein at its isoelectric point.
  • proteins produced in this way include casein, lactoglobulin, and lactalbumin, which are extracted from recombinant proteins derived from bacteria such as Escherichia coir, yeasts such as Saccharomyces cerevisiae and P. pastoris and fungi such as Rhizopus (10).
  • proteins that may be used in the compositions and/or methods provided herein include, but are not limited to, soy proteins such as glycinin and beta-conglycinin; wheat proteins, such as gliadin and glutenin; mung bean proteins, such as vignins, phaseolins, and globulins; maize proteins, such as zein; milk proteins, such as whey; egg proteins, such as albumin; epidermal proteins, such as keratin, gelatin and collagen; and insect proteins, such as resilin.
  • soy proteins such as glycinin and beta-conglycinin
  • wheat proteins such as gliadin and glutenin
  • mung bean proteins such as vignins, phaseolins, and globulins
  • maize proteins such as zein
  • milk proteins such as whey
  • egg proteins such as albumin
  • epidermal proteins such as keratin, gelatin and collagen
  • insect proteins such as resilin.
  • polypeptides derived from bacteria include, but are not limited to, casein, lactoglobulin and lactalbumin, which are extracted from the recombinant proteins derived from Escherichia coli.
  • casein lactoglobulin
  • lactalbumin which are extracted from the recombinant proteins derived from Escherichia coli.
  • chicken or cattle genes encoding casein, lactoglobulin or lactalbumin polypeptides may be expressed in E. coli (10).
  • proteins derived from yeasts include, but are not limited to, casein, lactoglobulin and lactalbumin, which are extracted from recombinant proteins derived from Saccharomyces cerevisiae and P. pastoris.
  • casein lactoglobulin
  • lactalbumin which are extracted from recombinant proteins derived from Saccharomyces cerevisiae and P. pastoris.
  • chicken or cow genes encoding casein, lactoglobulin or lactalbumin polypeptides may be expressed in Saccharomyces cerevisiae or P. pastoris (10).
  • proteins derived from other fungi include, but are not limited to, casein, lactoglobulin and lactalbumin, which are extracted from the recombinant proteins derived from the filamentous fungus Rhizopus.
  • casein lactoglobulin
  • lactalbumin which are extracted from the recombinant proteins derived from the filamentous fungus Rhizopus.
  • chicken or cow genes encoding casein, lactoglobulin or lactalbumin polypeptides may be expressed in Rhizopus (10).
  • polypeptides are used in a purified form or in combination with one or more other polypeptides and/or other materials.
  • the first composition may additionally comprise one or more polysaccharide(s).
  • the first composition additionally comprises one or more polysaccharide(s). In some embodiments, the first composition does not comprise polysaccharide(s).
  • the first composition may additionally comprise one or more polysaccharide(s). In certain other embodiments the first composition does not include polysaccharide(s).
  • the extract or isolate from a plant, animal, bacteria, algae, archaea, and/or fungi may additionally comprise one or more polysaccharide(s).
  • Polysaccharides are polymers of monosaccharides (simple sugars) linked with glycosidic bonds.
  • polysaccharide extracts may be obtained during the production of defatted beans, seeds or nut flakes. Suitable plant materials first undergo a process of cleaning, drying, conditioning, cracking, dehulling, solvent oil extraction and flash solvent removal. Polysaccharides are subsequently obtained by removing the proteins via alcohol washing, acid leaching and/or water leaching (9).
  • polysaccharides that could be used in addition to the polypeptides in the first composition include, but are not limited to, chitin, chitosan, starches derived from wheat, rice, potato or corn, alginate, agar, hyaluronic acid, dextran, chondroitin sulphate, carrageenan, carrageenan-kappa, carrageenan-iota, pullulan, xanthan gum, gellan gum, and/or pectin.
  • the first composition may additionally comprise one or more salt derivatives of polysaccharide(s).
  • salt derivatives of polysaccharide that could be used in addition to the polypeptides in the first composition include, but are not limited to, sodium alginate, potassium alginate, sodium carrageenan, and/or potassium carrageenan.
  • the polysaccharide may be used in a purified form or in combination with one or more other polypeptides, polysaccharide(s), lipid(s), and/or other materials.
  • the first composition may additionally comprise one or more lipid(s).
  • the first composition additionally comprises one or more lipid(s).
  • the first composition does not comprise lipids.
  • the first composition may additionally comprise one or more lipid(s).
  • the extract or isolate from a plant, animal, bacteria, algae, archaea, and/or fungi may additionally comprise one or more lipid(s).
  • Lipids are class of molecule that are highly diverse in their structure and function. Lipids are typically composed of fatty acids and glycerol, as well as other hydrocarbon chains.
  • lipids that could be used in addition to the polypeptides include, but are not limited to:
  • plant oils almond, avocado, canola, coconut, corn, flaxseed, grape seed, hemp seed, jojoba, mustard, olive, palm, peanut, pumpkin seed, rice bran, safflower, sesame, soybean, sunflower, walnut
  • animal fats including milks, butters, lards, and tallows: cattle, sheep, pig, horse, goat, deer, reindeer, bison, moose, elk, camel, wild boar, guinea pig, kangaroo, chicken, turkey, duck, goose, quail, pheasant, guineafowl, ostrich, emu, pigeon, partridge, grouse, snipe, woodcock, salmon, tuna, cod, trout, sardines, haddock, tilapia, catfish, mackerel, swordfish, halibut, dolphinfish, grouper, snapper, sea bass, anchovy, carp, perch, pike, flounder, sole, eel, herring, whitefish, and/or crawfish;
  • the lipids may be used in a purified form or in combination with one or more other polypeptide(s), polysaccharide(s), lipid(s), and/or other material(s).
  • the first composition may additionally comprise one or more polyol(s) and/or polymers of polyols.
  • the first composition additionally comprises one or more polyol(s) and/or polymers of polyols.
  • the first composition does not comprise polyols and/or polymers of polyols.
  • Polyols are organic compounds characterised in having multiple hydroxyl (-OH) groups, commonly bonded to carbon atoms, in their molecular structure.
  • polyols may include, but are not limited to: Ethylene glycol, Glycerol, Erythritol, Threitol, Arabitol, Xylitol, Ribitol Mannitol, Sorbitol, Galactitol, Fucitol, Iditol, Inositol, Volemitol, Isomalt, Maltitol, Lactitol, Maltotriitol, Maltotetraitol, Polyglycitol, monoacylglycerols, diacylglycerols and/or triacylglycerols.
  • the first composition additionally comprises polymers of polyols, such as, but not limited to, polyethylene glycol.
  • the first composition does not comprise polymers of polyols, such as, but not limited to, polyethylene glycol.
  • soy protein isolate as obtained from soybeans, are used for the production of the semi-permeable, porous hollow fibres.
  • SPI soy protein isolate
  • Soy protein extract is the most highly- refined soy protein product commercially available, with some commercial products comprising more than 90 % protein (by mass). Soy protein extract is produced from defatted soybean flakes wherein the majority of fat, sugars and fibre have been removed to leave protein as the prominent remaining component.
  • SPI may in some embodiments be produced through the extraction of protein in a mild alkali solution.
  • the extract is then isolated through centrifugation and subsequently acidified to produce protein curd.
  • the curd is washed to remove soluble sugars, neutralised and finally spray-dried (9).
  • Other plant protein isolates may be produced and used in a similar manner.
  • the polypeptides of the compositions and/or methods provided herein comprise soybean polypeptides.
  • the polypeptides consist essentially of soybean polypeptides.
  • the first composition comprises, or consists essentially of, polypeptides, wherein at least 60 %, preferably at least 75 %, of the polypeptides are soybean polypeptides (e.g., extracted from soybeans).
  • Step (a) the first composition comprising polypeptides is combined with a second composition comprising a solvent and one or more denaturing agents and/or reducing agents, to produce a third composition.
  • the first composition may additionally comprise one or more polysaccharide(s), lipid(s), polyol(s), polymer(s) of polyol, and/or any combination thereof.
  • the first composition additionally comprises one or more polysaccharide(s).
  • the first composition additionally comprises one or more lipid(s).
  • the first composition additionally comprises one or more polyol(s) and/or polymers of polyols. In some embodiments, the first composition additionally comprises one or more polysaccharide(s) and lipid(s).
  • the first composition additionally comprises one or more lipid(s), and polyol(s) and/or polymers of polyols.
  • 5 % to 35 % (weight per volume) of the first composition is added to the second composition.
  • the first composition is added to the second composition to a final concentration of 5 % to 35 % (w/v of the second composition).
  • the percentage of polypeptide in the third composition ranges from 5 % to 10 %, 10 % to 20 %, or 20 % to 35 % (weight per volume).
  • the first composition is added to the second composition to a final concentration of 5 % to 60 % (w/v of the second composition).
  • the percentage of polypeptide in the third composition ranges from 35 % to 45 %, 45 % to 55 %, or 55 % to 60 % (weight per volume).
  • polysaccharide(s) are added to the first composition in a ratio that is within the range of 1 % to 10000 % (weight per weight of polypeptide).
  • lipid(s) are added to the first composition (i) in a ratio that is within the range of 1 % to 10000 % (weight per weight of polypeptide).
  • polyol(s) and/or polymer(s) of polyols are added to the first composition (i) in a ratio that is within the range of 1 % to 10000 % (weight per weight of polypeptide).
  • the second composition comprises one or more solvents and one or more denaturing agents.
  • the second composition also comprises one or more reducing agents. In some embodiments, the second composition is capable of dissolving some or most of the polypeptides in the first composition.
  • the second composition is capable of dissolving all or substantially all of the polypeptides in the first composition.
  • the second composition is capable of achieving the complete dissolution of the polypeptides (and other components, e.g., polysaccharides, lipids, polyols, polymers of polyols, and/or salts) in the first composition.
  • the aim of some embodiments is to produce a fourth composition which is a viscous, and extrudable or spinnable composition.
  • Complete dissolution refers to a process wherein a substance, sometimes referred to as a solute, is completely dissolved into another substance, sometimes referred to as a solvent.
  • Complete dissolution can be experimentally verified via visible light spectroscopy absorbance measurements. An absorbance measurement can be taken for several samples, complete dissolution can be verified if all of measurements are uniform.
  • the second composition is one which is capable of breaking disulphide bonds and/or hydrogen bonds in at least one polypeptide in the first composition.
  • solvents which may be used in the second composition include aqueous solutions and/or organic solutions.
  • aqueous solutions include, aqueous acids, aqueous alkalis and/or aqueous salt solutions.
  • organic solutions include, but are not limited to, alcohols.
  • solvents used in the second composition may include water, ethanol, acetic acid, propanol and/or formaldehyde, and/or mixtures thereof.
  • a solvent used in the second composition is used either alone or in combination with other solvents.
  • the solvent used in the second composition is water.
  • one or more salts are added to the second composition in order to aid dissolution of the polypeptides (and other components, e.g., polysaccharides, lipids, when present) in the first composition.
  • salts include, but are not limited to, sodium chloride, potassium chloride, zinc chloride, sodium carbonate, potassium carbonate, sodium bicarbonate, and/or zinc sulphate.
  • one or more salts are added to the second composition in order to aid dissolution of the polypeptides (and other components, e.g., polysaccharides, lipids, when present) in the first composition.
  • salts include, but are not limited to, lithium sulphate, sodium malonate, sodium maleate, potassium sodium tartrate, triammonium citrate, trilithium citrate, trisodium citrate, tripotassium citrate, disodium hydrogen phosphite, sodium hypophosphite, monosodium phosphate, dipotassium phosphate, sodium sulphate, sodium bisulphate, potassium sulphate, ammonium sulphate, or sodium sulphite.
  • one or more salts are added to the second composition in order to aid dissolution of the polypeptides (and other components, e.g., polysaccharides, lipids, when present) in the first composition.
  • salts include, but are not limited to, sodium chloride, potassium chloride, zinc chloride, sodium carbonate, potassium carbonate, sodium bicarbonate, zinc sulphate, lithium sulphate, sodium malonate, sodium maleate, potassium sodium tartrate, triammonium citrate, trilithium citrate, trisodium citrate, tripotassium citrate, disodium hydrogen phosphite, sodium hypophosphite, monosodium phosphate, dipotassium phosphate, sodium sulphate, sodium bisulphate, potassium sulphate, ammonium sulphate, or sodium sulphite.
  • one or more salts are added to the second composition are hydrous and/or anhydrous.
  • the concentration of each salt which is added to the second composition is within the range of 0.1 % to 70 % (weight by weight of the polypeptides and other components, e.g., polysaccharides, lipids, when present); preferably, 0.1 % to 50 %; more preferably, 0.5 % to 10 %; even more preferably, 1 % to 7 %; and most preferably 1 % to 2 %.
  • concentration of each salt which is added to the second composition is within the range of 0.1 to 1 %, 1 % to 10 %, 10 % to 25 %, 25 % to 50 % or 50 % to 70 %.
  • the second composition may also comprise one or more denaturing agents and/or one or more reducing agents to solubilise and denature the polypeptides.
  • the second composition also comprises one or more denaturing agents to denature the polypeptides of the first composition.
  • the second composition also comprises one or more reducing agents to reduce the polypeptides of the first composition.
  • the second composition also comprises one or more denaturing agents and/or one or more reducing agents to solubilise and denature the polypeptides of the first composition.
  • the second composition does not comprise reducing agents.
  • the second composition does not comprise denaturing agents. In some embodiments, the second composition does not comprise denaturing agents or reducing agents.
  • the structure of a protein may be referred to in terms of its primary, secondary, and tertiary structures.
  • the primary structure of a protein is the linear sequence of amino acids linked together via peptide bonds to form a polypeptide backbone.
  • the secondary structure of a protein refers to the local folding of the polypeptide backbone into specific shapes and patterns that are stabilized by hydrogen bonding between amino acids which are close to each other in a three-dimensional space.
  • the tertiary structure of a protein refers to three-dimensional shape of a protein which develops due to the interaction of distant amino acids in a three-dimensional space (11).
  • Beta-helices are spiral structures held together by hydrogen bonds between the amino acids in the polypeptide backbone.
  • Beta-sheets are flat structures in which hydrogen bonds between adjacent protein chains result in the formation of pleated or accordionlike structures.
  • Beta-helices are helical structures held together by hydrogen bonding between adjacent proteins.
  • Amorphous, disordered, or denatured, used interchangeably herein, are protein structures which lack a well-defined or ordered secondary structure (11).
  • the primary, secondary and tertiary structures of polypeptides may be disrupted by applying appropriate agents to denature and/or reduce those polypeptides.
  • Alkali and acid solvents have traditionally been used to solubilise and denature polypeptides, but these solvents may also disrupt the primary structure, in addition to the secondary and tertiary structures.
  • aqueous urea may solubilise polypeptides, and when used together with reducing agents may denature the secondary and tertiary structures, without disrupting the primary structure (12).
  • the disruption of the secondary and tertiary structures may enable the solvation and stabilisation of the polypeptide backbone structures.
  • the linear nature of the unfolded molecular backbones may facilitate the lamination of the molecules in solution, which decreases the viscosity of the solution.
  • the linear nature of the molecules in solutions may ensure chain entanglement and alignment to enable the realisation of appropriate mechanical properties.
  • the degree of polypeptide chain entanglement may be maximised (12).
  • solubilisation of polypeptides with acids and alkalis is non-preferred, while solubilisation and denaturation with reducing agents is preferred.
  • the denaturation and reduction of polypeptides may be achieved by treatment with a number of chemical agents.
  • Urea is widely used to denature polypeptides for proteomic and metabolomic research, as the secondary and tertiary structures may be disrupted without the destruction of the molecular backbone. Urea may solubilise polypeptides through the interaction of hydrophobic motifs on the surface of the polypeptide tertiary structure to expose and solvate hydrophilic motifs in the protein structure (13).
  • urea is used in the second composition in order to solubilise the polypeptides of the first composition in a concentration that is within the range of 0 mol/L to 8, mol/L; 1 mol/L to 8 mol/L; preferably, within the range of 4 mol/L to 8 mol/L or 6 mol/L to 8 mol/L; and most preferably, the concentration is about 8 mol/L.
  • the concentration of urea is within the range of 0 mol/L to 1 mol/L, 1 mol/L to 2 mol/L, 2 mol/L to 4 mol/L, 4 mol/L to 6 mol/L or 6 mol/L to 8 mol/L.
  • Treatment with acid or alkali may also be used to denature and solubilise polypeptides, such as those in the solvent of the second composition in Step (a).
  • acid and alkali chemical treatments may disrupt the primary structure of the treated polypeptides (14).
  • acids which may be used as denaturing agents include, but are not limited to, oxalic acid, malic acid, succinic acid, adipic acid, tartaric acid, citric acid, malonic acid, acetic acid, formic acid, sulphuric acid, nitric acid, and/or hydrochloric acid.
  • alkalis which may be used as denaturing agents include, but are not limited to, sodium hydroxide, potassium hydroxide, calcium hydroxide, and/or magnesium hydroxide.
  • suitable denaturing agents include, but are not limited to, guanidine hydrochloride, sodium dodecyl sulphate (SDS), Triton X-100, ethanol, acetone, dimethyl sulfoxide (DMSO) sophorolipids, chaotropic salts (e.g., ammonium sulphate) and enzymes. Heat may also be used.
  • Reducing agents may disrupt disulphide bonds in the polypeptides of the first composition and prevent their reformation by inhibiting their oxidation, thereby aiding the disruption of the secondary and tertiary structures and the formation of a linear molecular backbone.
  • Suitable reducing agents include, but are not limited to, N- acetyl-cysteine, L-cysteine, glutathione, ascorbic acid, citric acid, tartaric acid, malic acid, sodium borohydride, sodium sulphite, sodium bisulphite, sodium metabisulphite, sodium hypophosphite, sodium hydrosulphite, mercaptoethanol and dithiothreitol.
  • one or more denaturing agents, reducing agents, and/or combinations thereof are included with the solvent in the second composition in order to aid the solvation of the polypeptides of the first composition to form the third composition.
  • the concentration of each denaturing and/or reducing agent are within the range of 0.01 to 50 % (by mass) of the polypeptides in the first composition and are included with the solvent of the second composition.
  • the concentration of the denaturing and/or reducing agents in the second composition is within the range of 0.01 to 0.1 %, 0.1 to 1 %, 1 % to 10 %, 10 % to 25 %, or 25 % to 50 % (by mass) of the polypeptides in the first composition.
  • the concentration of each denaturing and/or reducing agent are within the range of 0.01 to 1500 % (by mass) of the polypeptides in the first composition and are included with the solvent of the second composition.
  • the concentration of the denaturing and/or reducing agents in the second composition is within the range of 50 to 75 %, 75 to 100 %, 100 % to 200 %, 200 % to 300 %, 300 % to 400 %, 400 % to 500 %, 500 % to 600 %, 600 % to 700 %, 700 % to 800 %, 800 % to 900 %, 900 % to 1000 %, or 1000 % to 1500 %, (by mass) of the polypeptides in the first composition.
  • the pH of the second composition is within the range of 5.01 to 9.99; preferably, within the range of 6.0 to 9.0; and most preferably, within the range of 6.5 to 8.5.
  • the pH of the second composition is within the range of 5.01 to 6.0; 6.0 to 7.0, 7.0 to 8.0, 8.0 to 9.0, or 9.0 to 9.99.
  • the pH of the second composition is within the range of 9.0 to 9.99, 9.1 to 9.8, 9.2 to 9.7, or 9.3 to 9.4.
  • the first composition comprises, consists essentially of, or consists of, soybean polypeptides.
  • the solvent in the second composition is water.
  • the denaturing agents and/or reducing agents in the second composition comprise urea, N-acetyl-cysteine and/or sodium sulphite.
  • the pH of the second composition is within the range of 8.0 to 8.5;
  • the pH of the second composition is within the range of 9.0 to 9.5;
  • Step (a) comprises combining:
  • a second composition comprising water, and one or more denaturing and/or reducing agents selected from urea, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.0 to 8.5; to produce a third composition.
  • Step (a) comprises combining: (i) a first composition comprising, consisting essentially of, or consisting of, soybean polypeptides with,
  • a second composition comprising water, and one or more denaturing and/or reducing agents selected from urea, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.5 to 9.5; to produce a third composition.
  • Step (a) comprises combining:
  • a second composition comprising water, and one or more salts, denaturing and/or reducing agents selected from urea, sodium bicarbonate, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.0 to 8.5; to produce a third composition.
  • Step (a) comprises combining:
  • a second composition comprising water, and one or more salts, denaturing and/or reducing agents selected from urea, sodium bicarbonate, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.5 to 9.5; to produce a third composition.
  • Step (a) comprises combining:
  • composition comprising, consisting essentially of, or consisting of, mung bean polypeptides with,
  • a second composition comprising water, and one or more salts, denaturing and/or reducing agents selected from urea, sodium bicarbonate, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.0 to 8.5; to produce a third composition.
  • Step (a) comprises combining:
  • a first composition comprising, consisting essentially of, or consisting of, mung bean polypeptides with, (ii) a second composition comprising water, and one or more salts, denaturing and/or reducing agents selected from urea, sodium bicarbonate, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.5 to 9.5; to produce a third composition.
  • Step (a) comprises combining:
  • a first composition comprising, consisting essentially of, or consisting of, chickpea polypeptides with,
  • a second composition comprising water, and one or more salts, denaturing and/or reducing agents selected from urea, sodium bicarbonate, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.0 to 8.5; to produce a third composition.
  • Step (a) comprises combining:
  • a first composition comprising, consisting essentially of, or consisting of, chickpea polypeptides with,
  • a second composition comprising water, and one or more salts, denaturing and/or reducing agents selected from urea, sodium bicarbonate, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.5 to 9.5; to produce a third composition.
  • Step (a) comprises combining:
  • composition comprising, consisting essentially of, or consisting of, sunflower seed polypeptides with,
  • a second composition comprising water, and one or more salts, denaturing and/or reducing agents selected from urea, sodium bicarbonate, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.0 to 8.5;
  • Step (a) comprises combining:
  • composition comprising, consisting essentially of, or consisting of, sunflower seed polypeptides with,
  • Step (ii) a second composition comprising water, and one or more salts, denaturing and/or reducing agents selected from urea, sodium bicarbonate, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.5 to 9.5;
  • Step (a) comprises combining:
  • a second composition comprising water, and one or more salts, denaturing and/or reducing agents selected from urea, sodium bicarbonate, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.0 to 8.5;
  • Step (a) comprises combining:
  • a second composition comprising water, and one or more salts, denaturing and/or reducing agents selected from urea, sodium bicarbonate, N-acetyl-cysteine and sodium sulphite, at a pH that is within the range of 8.5 to 9.5;
  • the first composition of any embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • the second composition of any embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • the third composition of any embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • Step (b) the third composition is incubated under conditions that may be sufficient to solubilise, and denature and/or reduce at least a fraction of the polypeptides of the first composition, to form the fourth composition.
  • the viscosity of the third composition may be decreased, and the secondary and tertiary structures of the polypeptides of the first composition may be disrupted.
  • the third composition in Step (b), is incubated under conditions that are sufficient to solubilise, and denature and/or reduce at least a fraction of the polypeptides of the first composition, to form the fourth composition.
  • the third composition is incubated for a duration that is within the range of 0 minutes to 7 days; preferably, 10 minutes to 4 days; more preferably, 15 minutes to 1 days; most preferably, 20 minutes to 6 hours.
  • the third composition is incubated for a period of time that is within the range of 0 minutes to 5 minutes, 5 minutes to 10 minutes, 10 minutes to 20 minutes, 20 min to 1 hour, 1 hour to 3 hours, 3 hours to 6 hours, 6 hours to 12 hours, 12 hours to 1 day, 1 day to 2 days, 2 days to 3 days, 3 days to 4 days, 4 days to 5 days, 5 days to 6 days, or 6 days to 7 days.
  • the third composition is incubated at a temperature that is within the range of -25 °C to 150 °C; or within the range of 15 °C to 90 °C; or within the range of 20 °C to 90 °C.
  • the third composition is incubated at a temperature that is within the range of -25 °C to 0 °C, 0 °C to 25 °C, 25 °C to 50 °C, 50 °C to 75 °C, 75 °C to 100 °C, 100 °C to 125 °C, or 125 °C to 150 °C.
  • the third composition is incubated at room temperature.
  • the pH of the third composition is within the range of 5.01 to 9.99; preferably, within the range of 6.0 to 9.0; and most preferably, within the range of 6.5 to 8.5.
  • the pH of the third composition is within the range of 5.01 to 6.0; 6.0 to 7.0, 7.0 to 8.0, 8.0 to 9.0, or 9.0 to 9.99.
  • Mixing is herein defined as a mechanical process used to combine different substances or components, typically in a liquid or a homogeneous mixture. Mixing involves the thorough blending of two or more substances to create a uniform composition, ensuring that the individual components are evenly distributed throughout the mixture.
  • the third composition may additionally be mixed.
  • the third composition may additionally be mixed with a helical ribbon impeller.
  • the third composition is mixed during the incubation step.
  • the third composition is mixed during the incubation step with a helical ribbon impeller.
  • the third composition is not mixed during the incubation step.
  • the third composition is mixed for a period of time that is within the range of 0 minutes to 5 minutes, 5 minutes to 10 minutes, 10 minutes to 20 minutes, 20 min to 1 hour, 1 hour to 3 hours, 3 hours to 6 hours, 6 hours to 12 hours, 12 hours to 1 day, 1 day to 2 days, 2 days to 3 days, 3 days to 4 days, 4 days to 5 days, 5 days to 6 days, or 6 days to 7 days.
  • the third composition is mixed throughout a portion of, the majority of, or the entirety of the incubation step.
  • the third composition is mixed at a speed that is within the range of 0 rpm to 20,000 rpm; preferably 50 rpm to 10,000 rpm, more preferably 100 rpm to 5,000 rpm, even more preferably 200 rpm to 2,500 rpm, and most preferably 400 rpm to 1 ,000 rpm.
  • the third composition is mixed at a speed that is within the range of 0 rpm to 5 rpm, 5 rpm to 50 rpm, 50 rpm to 100 rpm, 100 rpm to 150 rpm, 150 rpm to 200 rpm, 200 rpm to 300 rpm, 300 rpm to 400 rpm, 400 rpm to 500 rpm, 500 rpm to 600 rpm, 600 rpm to 700 rpm, 700 rpm to 800 rpm, 800 rpm to 900 rpm, 900 rpm to 1 ,000 rpm, 1 ,000 rpm to 1 ,100 rpm, 1 , 100 rpm to 1 ,200 rpm, 1 ,200 rpm to 1 ,300 rpm, 1 ,300 rpm to 1 ,400 rpm, 1 ,400 rpm to 1 ,500 rpm, 1 ,500 rpm to 1 ,600 rpm, 1
  • the third composition is mixed at a speed that is within the range of 100 rpm to 400 rpm.
  • Room temperature may be a temperature within the range of 15 °C to 30 °C.
  • room temperature is a temperature within the range of 15 °C to 30 °C, preferably 18 °C to 25 °C, more preferably 19 °C to 22 °C and most preferably 20 °C.
  • the third composition is mixed a speed that is within the range of 400 rpm for 20 minutes with a helical ribbon impeller.
  • the viscosity of the fourth composition in some embodiments is within the range of 100 cP to 200,000 cP, preferably 100 cP to 1000 cP, 1000 cP to 5,000 cP, 5,000 cP to 10,000 cP, 10,000 cP to 50,000 cP, 50,000 cP to 100,000 cP or 100,000 cP to 200,000 cP, 200,000 cP to 300,000 cP, 300,000 cP to 400,000 cP, 400,000 cP to 500,000 cP, 500,000 cP to 600,000 cP, 600,000 cP to 700,000 cP, 700,000 cP to 800,000 cP, 800,000 cP to 900,000 cP, 900,000 cP, 900,000 cP, 900,000 cP, 900,000 cP, 900,000 cP to 1 ,000,000 cP, 1 ,000,000 cP to 1 ,100,000 cP,
  • Brookfield rotational viscometers are the most common device used in the evaluation of sample viscosities. Brookfield rotational viscometers measure the torque required to rotate a spindle in a fluid. For a Newtonian fluid, as the rate of spindle rotation or the surface area of the spindle used increases, the torque required will increase linearly. By evaluating the viscosity of a fluid at a given shear rate and temperature, a characteristic apparent viscosity may be measured.
  • Fluids may be Newtonian, in which shear rate and viscosity are linearly proportional; shear thinning, where the apparent viscosity decreases with an increase in shear rate; shear thickening, where the apparent viscosity increases with an increase in shear rate; a Bingham plastic fluid, which requires a finite yield stress before they begin to flow but then exhibit a linear shear rate and apparent viscosity profile; or a Bingham pseudoplastic fluid, which require a finite yield stress before they flow and exhibit a non-linear shear rate and apparent viscosity profile (15).
  • Rotational viscometers produced by other manufacturers may be used to evaluate viscosity.
  • a fraction of the polypeptides in the third composition is denatured and/or reduced; preferably, this fraction is higher than 50 %; more preferably, this fraction is higher than 65 %; and most preferably, this fraction is higher than 80 %.
  • the fraction of the polypeptides in the third composition which are denatured and/or reduced is within the range of 50 % to 60 %, 60 % to 70 %, 70 % to 80 %, 80 % to 90 %, or 90 % to 99.99 %.
  • the fraction of the polypeptides in the third composition which are denatured and/or reduced is within the range of 0 % to 10 %, 10 % to 20 %, 20 % to 30 %, 30 % to 40 %, or 40 % to 50 %.
  • the denaturation and reduction of the polypeptides in the third composition may be quantified through the use of Fourier transform infrared (FTIR) spectroscopy, circular dichroism, wide- angle X-ray scattering (WAXS) or Raman spectroscopy.
  • FTIR Fourier transform infrared
  • WAXS wide- angle X-ray scattering
  • Raman spectroscopy By recording representative spectra of the starting organic species, changes in band intensity associated with disulphide bonds may be used to evaluate the degree of reduction. Similarly, changes in band intensity associated with alpha-helices and beta-sheets may be used to determine changes in protein secondary structure.
  • One or more void fraction components may be introduced into the first, second, third or fourth compositions to aid in the controlled formation of pores in extruded or spun hollow fibres.
  • Void fraction components may impart porosity by acting as pore templates. This may occur as the dope solution undergoes phase inversion and solidifies around particles of the void fraction component.
  • the extruded or spun hollow fibres may be left with a microstructure that consists of a pore morphology that is a negative cast of the original particles of the void fraction component.
  • the void inclusion elements may be in the form of a solid, liquid and/or gas.
  • the pore volume of a material may refer to the total available volume of open space or pores within the material.
  • the porosity of a material may refer to the portion of total material volume occupied by open space or pores, and may be characterised using methods such as mercury porosimetry.
  • the pore volume and/or porosity of a material may also be determined by image analysis. Images which may be used to determine the porosity of materials include those produced through scanning electron microscopy. By considering the ratio of black and white pixels in a binarized image, the porosity may be determined by one skilled in the art.
  • one or more void fraction components are introduced into the first, second, third or fourth compositions to aid in the controlled formation of pores in extruded or spun hollow fibres.
  • void inclusion elements include, but are not limited, to powdered, or ground calcium carbonate.
  • void inclusion elements include, crushed or ground ice.
  • void inclusion elements include, gases such as, but are not limited, air, nitrogen, oxygen, carbon dioxide, helium, neon, argon, xenon, argon, and/or mixtures thereof.
  • void inclusion elements include salts such as, but are not limited to, sodium chloride, and potassium chloride.
  • void inclusion elements include lipids such as, but are not limited to, plant oils (e.g., almond, avocado, canola, coconut, corn, flaxseed, grape seed, hemp seed, jojoba, mustard, olive, palm, peanut, pumpkin seed, rice bran, safflower, sesame, soybean, sunflower, walnut).
  • plant oils e.g., almond, avocado, canola, coconut, corn, flaxseed, grape seed, hemp seed, jojoba, mustard, olive, palm, peanut, pumpkin seed, rice bran, safflower, sesame, soybean, sunflower, walnut).
  • the lipids have secondary process functions that include, but are not limited to, acting as flavour enhancers and as plasticisers.
  • void inclusion elements include one or more polyols and/or polymers of polyols.
  • void inclusion elements include water soluble polymers such as, but are not limited to polyvinyl alcohol.
  • solid powdered calcium carbonate may be introduced into the second composition and/or third composition and/or fourth composition.
  • the fourth composition may then be extruded or spun into a coagulation bath.
  • Hydrogen ions in the coagulation bath donated by a dissolved acidic species (e.g., citric acid), may then react with the calcium carbonate to form calcium salts and carbon dioxide.
  • voids may be left behind where the calcium carbonate particles were previously.
  • salts such as sodium chloride
  • the fourth composition may then be extruded into an aqueous coagulation bath.
  • the excess aqueous solvent in the coagulation bath may then dissolve the salts and may leave voids in place of the salt particles.
  • a gas such as air
  • the fourth composition may then be extruded into an aqueous coagulation bath. During the coagulation process gas bubbles trapped in the third composition may then result in the formation of void space and pores.
  • pores may be formed via a process referred to as ‘emulsion templating’.
  • a lipid such as sunflower oil
  • a lipid such as sunflower oil
  • an oil-in-water emulsion or mixture may be formed, in which oil droplets are dispersed within an aqueous phase.
  • the polypeptides of the first composition may act as a surfactant to stabilise the emulsion.
  • the fourth composition may then be extruded or spun into a coagulation bath. Upon removal of the oil droplets from the resulting extruded or spun hollow fibres void spaces may be formed, which may act as pores.
  • the void fraction components are added to the first composition in Step
  • the void fraction components are added to the second composition in Step (a).
  • the void fraction components are added to the third composition before or during the incubation step in Step (b).
  • the void fraction components are added to the third composition after the incubation step in Step (b), and then mixed.
  • the void fraction components are added to the fourth composition in Step
  • the void fraction components are added to the fourth composition in Step (b).
  • void inclusion elements are added to the compositions in Step (a) or Step (b), such that the void inclusion elements comprise 1 % to 80 % of the fourth composition; preferably 20 % to 75 %; more preferably 30 % to 70 %; and most preferably 35 % to 65 % of the fourth composition.
  • void inclusion elements are added to the compositions in Step (a) or Step (b), such that the void inclusion elements comprise 1 % to 5 %, 5 % to 10 %, 10 % to 15 %, 15 % to 20 %, 20 % to 25 %, 25 % to 30 %, 30 % to 35 %, 35 % to 40 %, 40 % to 45 %, 45 % to 50 %, 50 % to 55 %, 55 % to 60 %, 60 % to 65 %, 65 % to 70 %, 70 % to 75 %, and/or 75 % to 80 % of the fourth composition.
  • one or more other components are added to the compositions in Step (a) or Step (b) to improve the flexibility and ductile properties of the semi-permeable, porous hollow fibres.
  • one or more plasticizers are added to the compositions in Step (a) and/or Step (b) to improve the flexibility and ductile properties of the semi-permeable, porous hollow fibres.
  • plasticizers may include lipids, polyols and/or polymers of polyols.
  • one or more lipid(s), polyol(s) and/or polymer(s) of polyols are added to the compositions of Step (a) and/or Step (b) as plasticisers.
  • one or more lipid(s) are added to the compositions of Step (a) and/or Step (b) as plasticisers.
  • one or more polyol(s) and/or polymer(s) of polyols are added to the compositions of Step (a) and/or Step (b) as plasticisers.
  • one or more plasticizer is added to any of the compositions in Step (a) or Step (b) at any stage. In a preferred embodiment, one or more plasticizer is added to the fourth composition in Step (b).
  • plasticisers are not added to the compositions of Step (a) and/or Step (b).
  • the plasticizers have secondary process functions that include, but are not limited to, acting as flavour enhancers and as void fraction components.
  • one or more plasticisers are added to the compositions in Step (a) and/or Step (b), such that they each comprise 1 % to 80 % of the fourth composition; preferably 20 % to 75 %; more preferably 30 % to 70 %; and most preferably 35 % to 65 % of the fourth composition.
  • one or more plasticisers are added to the compositions in Step (a) or Step (b), such that they each comprise 1 % to 5 %, 5 % to 10 %, 10 % to 15 %, 15 % to 20 %, 20 % to 25 %, 25 % to 30 %, 30 % to 35 %, 35 % to 40 %, 40 % to 45 %, 45 % to 50 %, 50 % to 55 %, 55 % to 60 %, 60 % to 65 %, 65 % to 70 %, 70 % to 75 %, and/or 75 % to 80 % of the fourth composition.
  • One or more other components may be added to the compositions of Step (a) and/or Step (b) as flavouring agents which may improve the flavour of the extruded or spun, semi-permeable, porous hollow fibres, comprising covalent ester, thioester and/or amide crosslinked polypeptides.
  • one or more other components are added to the compositions in Step (a) and/or Step (b) as flavouring agents to improve the flavour of the extruded or spun, semi- permeable, porous hollow fibres, comprising covalent ester, thioester and/or amide crosslinked polypeptides.
  • the flavouring agent(s) added to the compositions in Step (a) and/or Step (b) include, but are not limited to, one or more lipid(s), polyol(s), and/or polymer(s) of polyols.
  • flavouring agent(s) added to the compositions in Step (a) and/or Step (b) include, but are not limited to, those listed by the European Commission in (EC) No 1334/2008 established in 2012 with regulation EU 872/2012 and non-food sourced flavourings added in amendments in accordance with Regulation EU 2018/1259.
  • flavouring agents are not added to the compositions in Step (a) and/or Step (b).
  • compositions of Step (a) and/or Step (b) are mixed following the addition of at least one additive, including but not limited to, void inclusion elements, plasticisers, and/or flavourings.
  • composition(s) of Step (a) and/or Step (b) are degassed.
  • degassing also known as degasification, is defined as the partial or complete removal of gas bubbles from any of the compositions.
  • Controlled degassing may enable the removal of uncontrolled void space in the dope solution. This may ensure that pore size of the hollow fibres are controlled and uniform, which may enable the cell size exclusion partitioning of the lumen and extra-capillary space of a hollow fibre bioreactor for use in long term cell culture.
  • the third composition is degassed.
  • the fourth composition is degassed.
  • Degassing may be achieved via one or more methods which may include, but are not limited to, pressure reduction, thermal regulation, membrane degasification, ultrasonic degassing, freeze- pump-thaw cycling, and/or centrifugation. In some embodiments, degassing is achieved via centrifugation.
  • degassing is achieved via pressure reduction under vacuum.
  • degassing is achieved via ultrasonic degassing.
  • Step (b) the third composition is incubated for 2 days to 4 days.
  • Step (b) the third composition is incubated at a temperature between
  • Step (b) the third composition is incubated at room temperature.
  • Step (b) the third composition is incubated for 2 days to 4 days at a temperature between 18 °C and 25 °C.
  • Step (b) additionally comprises the step of introducing one or more void fraction components, preferably powdered calcium carbonate, into the fourth composition.
  • Step (b) additionally comprises the step of introducing one or more void fraction components, preferably a lipid, into the fourth composition.
  • Step (b) comprises: incubating the third composition for 3 days at a temperature between 18 °C and 25 °C, to produce a fourth composition; and introducing lipids into the fourth composition.
  • undissolved components of lower densities in the third composition may be removed prior to Step (c) by employing methods such as, but not limited to, centrifugal density-based separation (density gradient centrifugation) as performed via rotational centrifuge.
  • gases and undissolved components of lower densities in the third composition arise to the surface of the third composition, which may then be mechanically removed.
  • the fourth composition is mixed.
  • the fourth composition is incubated for 20 minutes and is mixed.
  • the fourth composition is degassed.
  • one or more lipid is added to the third composition to act as void inclusion element(s).
  • the third composition is mixed at 400 rpm and at room temperature with an overhead mixer equipped with a helical blade impeller for 20 minutes to form the fourth composition.
  • the fourth composition is then degassed via centrifugation.
  • one or more lipid is added to the third composition to act as void inclusion element(s).
  • the third composition is mixed at 1000 rpm and at room temperature with an overhead mixer equipped with a helical blade impeller for 20 minutes to form the fourth composition.
  • the fourth composition is then degassed via centrifugation.
  • one or more lipid is added to the third composition to act as void inclusion element(s).
  • the third composition is mixed at 2000 rpm and at room temperature with an overhead mixer equipped with a helical blade impeller for 20 minutes to form the fourth composition.
  • the fourth composition is then degassed via centrifugation.
  • the fourth composition of any embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • Step (c) comprises extruding or spinning the fourth composition through a plurality of co-axial orifices, together with a bore solution to produce a plurality of hollow fibres.
  • a bore solution is herein defined as a solution which fills the lumen of an extruded hollow fibre.
  • a bore solution may comprise an aqueous solution.
  • a die sometimes referred to as a spinneret and used interchangeably herein, consists of a one or more concentric cylinders fed by a corresponding number of inlet streams.
  • a die may be in the form of a spinneret or a plurality of co-axial orifices.
  • Extrusion may be used to create an extruded tubular cylindrical product which can be treated to produce hollow fibres of a fixed cross-sectional profile by pushing the fourth composition, together with a bore solution, through a plurality of co-axial orifices (e.g., a die) of the desired cross-section.
  • a plurality of co-axial orifices e.g., a die
  • a bore solution is a solution that is extruded in the inner hollow of the polymer solution, thereby filling and forming the lumen of hollow fibres of the disclosure.
  • a bore solution may be extruded together with the fourth composition to maintain the channel(s) in the hollow fibres.
  • a bore solution is extruded together with the fourth composition to maintain the channel(s) in the hollow fibres.
  • a bore solution may be formulated to facilitate the polycarboxylic acid derived ester, thioester or amide covalent bond crosslink mediated gelation, precipitation of the covalently-crosslinked hollow fibres, and to facilitate the formation of the extruded or spun hollow fibres, comprising covalent ester, thioester and/or amide crosslinked polypeptides.
  • the bore solution is a solution that fills the lumen of hollow fibres.
  • a bore solution is a solution that fills the lumen of hollow fibres and is formulated to facilitate the polycarboxylic acid derived ester, thioester or amide covalent bond crosslink mediated gelation, precipitation of the covalently-crosslinked hollow fibres, and to facilitate the formation of the extruded or spun hollow fibres, comprising covalent ester, thioester and/or amide crosslinked polypeptides.
  • the bore solution has the same composition as the coagulation bath solution.
  • the bore solution is drawn from the coagulation bath solution and has the same composition as the coagulation bath solution.
  • the bore solution does not have the same composition as the coagulation bath solution.
  • the bore solution comprises thickening agent(s) and/or viscosity modifier(s) to help maintain the channel(s) in the hollow fibres.
  • the thickening agent(s) and/or viscosity modifier(s) in the bore solution are polysaccharides, which include: chitin, chitosan, starches derived from wheat, rice, potato or corn, alginate, agar, hyaluronic acid, dextran, chondroitin sulphate, carrageenan, carrageenan- kappa, carrageenan-iota, pullulan, xanthan gum, gellan gum, and/or pectin.
  • polysaccharides include: chitin, chitosan, starches derived from wheat, rice, potato or corn, alginate, agar, hyaluronic acid, dextran, chondroitin sulphate, carrageenan, carrageenan- kappa, carrageenan-iota, pullulan, xanthan gum, gellan gum, and/or pectin.
  • the bore solution is removed after Step (d).
  • Step (c) comprises extruding and/or spinning the fourth composition together with a bore solution through a die comprising a plurality of co-axial orifices to produce a plurality of hollow fibres.
  • the hollow fibres may be tubular and/or cylindrical in shape.
  • the hollow fibres are tubular and/or cylindrical in shape.
  • the dimensions of the die are selected such as to produce hollow fibres having the dimensions specified herein.
  • the fourth composition is extruded through the die at a rate that is within the range of 0.1 mL/hour to 10 mL/hour; more preferably, within the range of 0.5 mL/hour to 5 mL/hour; and most preferably at 1 mL/hour.
  • the fourth composition is extruded through the die at a rate that is within the range of 0.1 mL/hour to 0.5 mL/hour, 0.5 mL/hour to 1 mL/hour, 1 mL/hour to 5 mL/hour, or 5 mL/hour to 10 mL/hour.
  • the fourth composition is extruded through the die at a rate that is within the range of 0.1 mL/hour to 100 mL/hour; preferably, within the range of 5 mL/hour to 75 mL/hour; more preferably, within the range of 10 mL/hour to 50 mL/hour; and most preferably at 12 mL/hour.
  • the fourth composition is extruded through the die at a rate that is within the range of 0.1 mL/hour to 10 mL/hour, 10 mL/hour to 20 mL/hour, 20 mL/hour to 50 mL/hour, 50 mL/hour to 75 mL/hour, or 75 mL/hour to 100 mL/hour.
  • the bore solution is extruded through the die at a rate that is within the range of 0.001 mL/hour to 10 mL/hour; more preferably, within the range of 0.05 mL/hour to 5 mL/hour; and most preferably at 1 mL/hour.
  • the bore solution is extruded through the die at a rate that is within the range of 10 mL/hour to 30 mL/hour; more preferably, within the range of 10 mL/hour to 20 mL/hour; and more preferably at 12 mL/hour.
  • the bore solution is extruded through the die at a rate that is within the range of 0.001 mL/hour to 0.05 mL/hour, 0.05 mL/hour to 0.1 mL/hour, 0.1 mL/hour to 0.5 mL/hour, 0.5 mL/hour to 1 mL/hour, 1 mL/hour to 5 mL/hour, or 5 mL/hour to 10 mL/hour.
  • the bore solution is extruded through the die at a rate that is within the range of 10 mL/hour to 15 mL/hour, 15 mL/hour to 20 mL/hour, 20 mL/hour to 25 mL/hour, or 25 mL/hour to 30 mL/hour.
  • the plurality of co-axial orifices comprises 2 to 4 orifices; preferably, 2 to 3 orifices; and most preferably, 2 orifices.
  • the plurality of co-axial orifices comprises 2 orifices, 3 orifices, or 4 orifices.
  • Each orifice of the plurality of co-axial orifices may have profiles that are circular, square, triangular, pentagonal, hexagonal and/or other polygons in shape.
  • each orifice of the plurality of co-axial orifices has a profile that is circular, square, triangular, pentagonal, hexagonal and/or other polygons in shape.
  • Dry or wet spinning systems may also be used depending on the composition of the spinning solutions that form the fourth composition.
  • Spinning, wet spinning, and/or extrusion are used interchangeably herein and refer to the production of a hollow fibre through a process in which a polymer solution may be passed through a die together with a bore solution, which may consist of a coagulation bath solution, into a coagulation bath in which the polymer solution may solidify into a hollow fibre.
  • the process may be referred to as “wet” spinning as the polymer solution is extruded into a liquid bath.
  • spinning, wet spinning, and/or extrusion may therefore refer to a process which comprises: (i) the preparation of a polymer solution; (ii) die/spinneret assembly; (iii) extrusion of the polymer solution together with a bore solution; (iv) coagulation of the extruded polymer solution; (v) drawing in which the extruded solidified hollow fibres are stretched; (vi) washing, in which residual solvents are removed; and (vii) drying in which the hollow fibres produced are dried and wound onto a spool for storage and future application.
  • the fourth composition together with a bore solution, is extruded or spun into air and/or a gaseous atmosphere to produce hollow fibres.
  • the extruded or spun hollow fibres are then directed either into a coagulation bath or into an atmosphere that coagulates, precipitates or dries the hollow fibres.
  • the fourth composition together with a bore solution, is extruded or spun into an atmosphere and then into a coagulation bath.
  • the fourth composition is extruded, togetherwith the bore solution, through a die directly into a coagulation bath.
  • Step (c) comprises extruding or spinning the fourth composition, togetherwith a bore solution comprising the coagulation bath solution, through a plurality of coaxial orifices to form one or more hollow fibres with tubular cylindrical geometry directly into a coagulation bath.
  • the fourth composition is extruded through the outer orifice and a bore solution is extruded through the inner orifice of a die with two concentric cylindrical orifices to form a hollow fibre with a tubular cylindrical geometry.
  • compositions of both the bore solution and coagulation bath solution include one or more crosslinking reagent(s), such as sodium citrate, and one or more salt(s), such as sodium hypophosphite.
  • compositions of both the bore solution and coagulation bath solution include one or more crosslinking reagent(s), such as sodium malate, and one or more salt(s), such as sodium hypophosphite.
  • compositions of both the bore solution and coagulation bath solution include one or more crosslinking reagent(s), such as sodium citrate.
  • compositions of both the bore solution and coagulation bath solution include one or more crosslinking reagent(s), such as sodium malate.
  • the pH of both the bore solution and coagulation bath solutions are within the range of 7.01 to 9.99; preferably, within the range of 8.0 to 9.0; and most preferably, within the range of 8.0 to 8.5.
  • Step (c) comprises extruding the fourth composition, together with a bore solution that has the same components of the coagulation bath solution, through a co-axial orifice, comprising two orifices of circular profile.
  • the fourth composition is extruded through the outer orifice and the bore solution through the inner orifice.
  • the fourth composition and the bore solution are both extruded directly into a coagulation bath filled with coagulation bath solution.
  • the coagulation bath solution and bore solution both comprise sodium citrate and sodium hypophosphite in an aqueous solution at a pH between 8 and 8.5.
  • Step (c) comprises extruding the fourth composition, together with a bore solution that has the same components of the coagulation bath solution, through a co-axial orifice, comprising two orifices of circular profile.
  • the fourth composition is extruded through the outer orifice and the bore solution through the inner orifice.
  • the fourth composition and the bore solution are both extruded directly into a coagulation bath filled with coagulation bath solution.
  • the coagulation bath solution and bore solution both comprise sodium malate in an aqueous solution at a pH between 8 and 8.5.
  • Step (d) comprises treating the plurality of hollow fibres, produced in Step (c), with a polycarboxylic acid crosslinking reagent which may form inter-polypeptide and/or intrapolypeptide polycarboxylic acid derived ester, thioester or amide covalent bond crosslinks within at least a fraction of the polypeptides comprising the hollow fibres, to produce a plurality of covalently-crosslinked, semi-permeable, porous hollow fibres;
  • a polycarboxylic acid crosslinking reagent which may form inter-polypeptide and/or intrapolypeptide polycarboxylic acid derived ester, thioester or amide covalent bond crosslinks within at least a fraction of the polypeptides comprising the hollow fibres
  • Step (d) comprises treating the plurality of hollow fibres with a polycarboxylic acid crosslinking reagent to form inter-polypeptide and/or intra-polypeptide polycarboxylic acid derived ester, thioester or amide covalent bond crosslinks within at least a fraction of the polypeptides comprising the hollow fibres, to produce a plurality of covalently- crosslinked, semi-permeable, porous hollow fibres;
  • Step (d) may be performed in a coagulation bath comprising a coagulation bath solution, as defined herein. In some embodiments, Step (d) is performed in a coagulation bath comprising a coagulation bath solution.
  • Step (d) may be carried out using multiple and/or consecutive coagulation baths, comprising coagulation bath solutions of the same composition and/or of different compositions.
  • Step (d) is carried out using multiple and/or consecutive coagulation baths, comprising coagulation bath solutions of the same composition and/or of different compositions.
  • thermodynamically-stable solution of the polypeptides may transform from a liquid state into a solid state in a controlled manner.
  • a polypeptide-rich solution such as the extruded or spun fourth composition from Step (c)
  • a polypeptide-lean solution such as the coagulation bath solution
  • the phase rich in polypeptides may start to solidify through processes such as gelation, crosslinking, vitrification and/or crystallisation.
  • Separation may be induced in a number of ways, including: (i) immersion precipitation, whereby liquid-liquid interactions between the solvent and non-solvent may lead to the precipitation of the polypeptide; (ii) controlled evaporation of the solvent to potentially isolate the polypeptides; and (iii) gelation, in which bond formation between the polypeptide molecules may be induced during liquid-liquid transfer between the solvent and non-solvent by applying either crosslinking agents and sometimes a catalyst, or altering the isoelectric point with salts (16) or through salting out with Hoffmeister series salts (17).
  • Covalently crosslinking polypeptides with polycarboxylic acid derived ester, thioester or amide bonds may lead to the formation of larger polypeptide aggregates.
  • the formation of larger polypeptide aggregates in the extruded or spun hollow fibres may decrease both the water solubility and elasticity of the hollow fibres and increase both their material stiffness and tensile strength (9).
  • the structure and structural integrity of hollow fibres may be altered in Step (d) as to potentially enable the hollow fibres to be used as components within a bioreactor for a prolonged period, as may be required for the cultivation of cells for the production of cultured meat.
  • the precipitation of the solid phase in the coagulation bath may form porous structures in the solid phase.
  • the principal parameters which may define the porosity of the precipitate are the concentration of the polymer in the polymer-rich phase (i.e., the concentration of the polypeptide in the fourth composition) and the rate at which the precipitate forms.
  • concentration of the polymer in the polymer-rich phase i.e., the concentration of the polypeptide in the fourth composition
  • rate at which the precipitate forms i.e., the rate at which the precipitate forms.
  • non-solvent precipitation the rapid de-mixing of a binary solvent and solute system, upon the addition of a miscible tertiary non-solvent liquid and under favourable thermodynamic conditions may lead to spinodal precipitation potentially resulting in pore formation.
  • rapid de-mixing and precipitation of the solute into the solid phase yields a more porous precipitate.
  • Such an occurrence may be characterised by a rapid colour change or onset of turbidity in the solute rich phase (16) (18).
  • void inclusion elements may be added to the polymer solution prior to extrusion, and subsequently removed from the precipitate to potentially increase the void fraction of the precipitate (19).
  • the void inclusion element is a lipid, such as sunflower oil, and is used to form an oil-in-water emulsion with the polymer solution to create controlled porous structures, the process may be referred to as ‘emulsion templating’.
  • Covalent crosslinking may be carried out through the reaction of polycarboxylic acids with the reactive groups on the polypeptide backbone, including amine (-NH 2 ), hydroxide (-OH), carboxyl (-COOH) and thiol (-SH) groups, to potentially form ester, thioester or amide covalent bond crosslinks.
  • amine -NH 2
  • hydroxide -OH
  • carboxyl -COOH
  • thiol thiol
  • Covalent crosslinking may be carried out through the reaction of polycarboxylic acids with the reactive groups in polypeptides and polysaccharides, including amine (-NH 2 ) hydroxide (-OH), carboxyl (-COOH) and thiol (-SH) groups, to potentially form polypeptide-polypeptide, polypeptides-polysaccharide, polysaccharide-polysaccharide ester, thioester and/or amide covalent bond crosslinks.
  • polycarboxylic acids with the reactive groups in polypeptides and polysaccharides, including amine (-NH 2 ) hydroxide (-OH), carboxyl (-COOH) and thiol (-SH) groups, to potentially form polypeptide-polypeptide, polypeptides-polysaccharide, polysaccharide-polysaccharide ester, thioester and/or amide covalent bond crosslinks.
  • covalent ester, thioester and/or amide bond crosslinked polypeptide means a polypeptide having at least one or more ester, thioester and/or amide crosslinking bond. It is generally understood that any such ester, thioester and/or amide crosslinking bond would be derived from a polycarboxylic acid. As such, the term “polycarboxylic acid derived covalent ester, thioester and/or amide bond crosslinked polypeptides” encompasses any covalent ester, thioester and/or amide bond crosslinked polypeptide without the need for demonstrating that it was actually derived from a polycarboxylic acid.
  • covalent ester, thioester and/or amide bond crosslinked polypeptide and “polycarboxylic acid derived covalent ester, thioester and/or amide bond crosslinked polypeptides” are used interchangeably herein.
  • the covalent crosslinking of the polypeptides in the hollow fibres produced in Step (c) is performed via non-specific esterification, thio-esterification or amidation reactions involving the use of polycarboxylic acid salts as non-harsh chemical crosslinking agents.
  • the covalent crosslinking of polypeptides and polysaccharides in the hollow fibres produced in Step (c) is performed via non-specific esterification, thio- esterification or amidation reactions involving the use of polycarboxylic acid salts as chemical crosslinking agents.
  • non-harsh chemical refers to a chemical which is designated as GRAS.
  • the covalent crosslinking of polypeptides and polysaccharides in the hollow fibres produced in Step (c) is performed via non-specific esterification, thio- esterification or amidation reactions involving the use of polycarboxylic acid salts as non-harsh chemical crosslinking agents.
  • the polycarboxylic acid salts used to form the covalently-crosslinks in the hollow fibres, produced in Step (c), include, but are not limited to, sodium oxalate, potassium oxalate, sodium malate, potassium malate, sodium succinate, potassium succinate, sodium adipate, potassium adipate, sodium tartrate, potassium tartrate, potassium citrate, sodium citrate, sodium malonate, potassium malonate, and/or combinations thereof; and preferably, sodium citrate.
  • covalent crosslinking with polycarboxylic salts with more than one carboxyl groups is carried out under alkaline conditions leading to the formation of non-specific ester, thioester or amide -bond covalent crosslinks (20).
  • alkaline conditions may allow for the covalent crosslinking of polypeptides at low temperatures, which may increase the energy efficiency of the production process and negate the potential yellowing of materials seen when operating at higher temperatures (20).
  • polycarboxylic acid salts with more than one carboxyl group form crosslinks in a solution comprising other salts, including, but not limited to, sodium hypophosphite (22), in which sodium hypophosphite acts as a catalyst or is integrated into the crosslinks (22; 23).
  • other salts including, but not limited to, sodium hypophosphite (22), in which sodium hypophosphite acts as a catalyst or is integrated into the crosslinks (22; 23).
  • One or more polycarboxylic acid salt crosslinking agents may be used.
  • a single polycarboxylic acid salt crosslinking agents is used.
  • one or more polycarboxylic acid salt crosslinking agents are used.
  • multiple polycarboxylic acid salt crosslinking agents are used in combination.
  • the concentration of each polycarboxylic acid salt crosslinking agent in the coagulation bath solution is within the range of 0.01 % (w/v) to 50 % (w/v); and preferably, within the range of 0.05 % (w/v) to 35 % (w/v); more preferably, within the range of 0.1 % (w/v) to 30 % (w/v); even more preferably, within the range of 1 % (w/v) to 25 % (w/v); and most preferably, within the range of 5 % (w/v) to 15 % (w/v), relative to the volume of the solution.
  • the concentration of each polycarboxylic acid salt crosslinking agent in the coagulation bath solution is within the range of 0.01 % (w/v) to 1 % (w/v), 1 % (w/v) to 5 % (w/v), 5 % (w/v) to 10 % (w/v), 10 % (w/v) to 15 % (w/v), 15 % (w/v) to 20 % (w/v), 20 % (w/v) to 25 % (w/v), 25 % (w/v) to 30 % (w/v), 30 % (w/v) to 35 % (w/v), 35 % (w/v) to 40 % (w/v), 40 % (w/v) to 45 % (w/v), or 45 % (w/v) to 50 % (w/v), relative to the volume of the solution.
  • the temperature used in the covalently crosslinking step is room temperature, or at temperatures that are either higher or lower than room temperature; preferably, in the range of 5 °C to 95 °C; more preferably, in the range of 10 °C to 75 °C; even more preferably, in the range of 15 °C to 65 °C; yet even more preferably, in the range of 20 °C to 60 °C; and most preferably, in the range of 30 °C to 55 °C.
  • the temperature used in the covalently crosslinking step is within the range of 5 °C to 10 °C, 10 °C to 20 °C, 20 °C to 30 °C, 30 °C to 50 °C, 50 °C to 75 °C, or 75 °C to 95 °C .
  • the pressure used in the covalently crosslinking step could be atmospheric pressure, or at pressures that are either higher or lower than atmospheric pressure; preferably, in the range of 90000 Pa to 109000 Pa; more preferably, in the range of 96400 Pa to 108400 Pa; and most preferably, in the range of 100825 Pa to 101825 Pa.
  • the pressure used in the covalently crosslinking step is in the range of 90000 Pa to 109000 Pa, 95000 Pa to 105000 Pa, or 100000 Pa to 102000 Pa.
  • the pH used in the covalently crosslinking step could be within the range of 7.01 to 9.99; preferably, within the range of 8.0 to 9.0; and most preferably, within the range of 8.0 and 8.5.
  • the pH used in the covalently crosslinking step is within the range of 7.01 to 7.5, 7.5 to 8.0, 8.0 to 8.5, 8.5 to 9.0, 9.0 to 9.5, and 9.5 to 9.99.
  • the duration of the covalent crosslinking step is within the range of 30 seconds and 6 hours; preferably, within the range of 10 minutes and 6 hours; more preferably, within the range of 20 minutes and 3 hours; even more preferably, within the range of 30 minutes and 2 hours; and most preferably, within the range of 45 minutes and 1 hour.
  • the duration of the covalently crosslinking step is within the range of 30 seconds to 60 seconds, 1 minute to 30 minutes, 30 minutes to 60 minutes, 1 hour to 2 hours, 2 hours to 3 hours, 3 hours to 4 hours, 4 hours to 5 hours, 5 hours to 6 hours. In some other embodiments, the duration of the covalent crosslinking step is within the range of 6 hours and 7 days; preferably, within the range of 6 hours and 72 hours; and more preferably, within the range of 12 hours and 24 hours.
  • the duration of the covalently crosslinking step is within the range of 30 seconds to 7 days, 24 hours to 96 hours, 30 seconds to 6 hours, 6 hours to 12 hours, 12 hours to 24 hours, 24 hours to 48 hours, 48 hours to 72 hours, 72 hours to 96 hours, or 96 hours and/or 7 days.
  • a coagulation bath solution of Step (d) may comprise at least one polycarboxylic acid salt crosslinking agent dissolved in an aqueous solvent or in a mixture of multiple solvents.
  • a coagulation bath solution of Step (d) comprises at least one polycarboxylic acid salt crosslinking agent dissolved in an aqueous solvent or in a mixture of multiple solvents.
  • a coagulation bath solution of Step (d) contains aqueous solvents, such as water.
  • a coagulation bath solution of Step (d) additionally comprises at least one, alkali, acid, alcohol, catalyst, organic solvent, salt, and/or any combination thereof.
  • a coagulation bath solution of Step (d) contains one or more polycarboxylic acid salt crosslinking agents, including, sodium oxalate, potassium oxalate, sodium malate, potassium malate, sodium succinate, potassium succinate, sodium adipate, potassium adipate, sodium tartrate, potassium tartrate, potassium citrate, sodium citrate, sodium malonate, potassium malonate, and/or any combination thereof.
  • polycarboxylic acid salt crosslinking agents including, sodium oxalate, potassium oxalate, sodium malate, potassium malate, sodium succinate, potassium succinate, sodium adipate, potassium adipate, sodium tartrate, potassium tartrate, potassium citrate, sodium citrate, sodium malonate, potassium malonate, and/or any combination thereof.
  • the concentration of each polycarboxylic acid salt crosslinking agent within a coagulation bath solution of Step (d) is within the range of 0.1 % (w/v) to 40 % (w/v); preferably, within the range of 10 % (w/v) to 30 % (w/v); more preferably, within the range of 20 % (w/v) to 28 % (w/v); and most preferably at 25 % (w/v), relative to the volume of the solution.
  • the concentration of each polycarboxylic acid salt crosslinking agent within a coagulation bath solution of Step (d) is within the range of 0.1 % (w/v) to 1 % (w/v), 1 % (w/v) to 5 % (w/v), 5 % (w/v) to 10 % (w/v), 10 % (w/v) to 20 % (w/v), and/or % (w/v) to 40 % (w/v), relative to the volume of the solution.
  • a coagulation bath solution of Step (d) contains catalysts which include zinc sulphate, sodium sulphite, sodium bisulphite, sodium meta-bisulphite, sodium hypophosphite and/or ammonium sulphate.
  • the concentration of each catalyst within a coagulation bath solution of Step (d) is within the range of 0.1 % (w/v) to 40 % (w/v); preferably, within the range of 5 % (w/v) to 15 % (w/v) more preferably, within the range of 10 % (w/v) to 14 % (w/v); and most preferably at 12.5 % (w/v) relative to the volume of the solution.
  • the concentration of each catalyst within a coagulation bath solution of Step (d) is within the range of 0.1 % (w/v) to 1 % (w/v), 1 % (w/v) to 5 % (w/v), 5 % (w/v) to 10 % (w/v), 10 % (w/v) to 20 % (w/v), and/or % (w/v) to 40 % (w/v) relative to the volume of the solution.
  • a coagulation bath solution of Step (d) contains alkalis which include, but are not limited to sodium hydroxide, potassium hydroxide, calcium hydroxide, magnesium hydroxide and/or sodium carbonate.
  • a coagulation bath solution of Step (d) contains alkalis which include, but are not limited to, ammonium hydroxide, potassium carbonate, and/or sodium bicarbonate.
  • a coagulation bath solution of Step (d) contains acids which include, but are not limited to, ascorbic acid, acetic acid, adipic acid, citric acid, formic acid, hydrochloric acid, lactic acid, malic acid, malonic acid, oxalic acid, succinic acid, sulphuric acid and/or tartaric acid.
  • the pH of a coagulation bath solution of Step (d) is within the range of 7.01 to 9.99; preferably, within the range of 8.0 to 9.0; and most preferably, within the range of 8.0 and 8.5.
  • the pH of a coagulation bath solution of Step (d) is within the range of 7.01 to 7.5, 7.5 to 8.0, 8.0 to 8.5, 8.5 to 9.0, 9.0 to 9.5, and 9.5 to 9.99.
  • a coagulation bath solution of Step (d) contains one or more alcohols which include, but are not limited to methanol, ethanol, propanol, iso-propanol, butanol and/or iso-butanol.
  • a coagulation bath solution of Step (d) contains alcohols with a concentration of 0.5 % to 5 % (v/v), 5 % to 10 % (v/v), 10 % to 30 % (v/v), 20 % to 30 % (v/v), 30 % to 40 % (v/v), 40 % to 50 % (v/v), 50 % to 60 % (v/v), 60 % to 70 % (v/v), 70 % to 80 % (v/v), 80 % to 90 % (v/v), or 90 % to 99.99 % (v/v), relative to the total coagulation bath solution volume.
  • a coagulation bath solution of Step (d) contains one or more salts including, but not limited to, ammonium sulphate, disodium hydrogen phosphite, dipotassium phosphate, lithium sulphate, monosodium phosphate, potassium carbonate, potassium chloride, potassium sulphate, sodium bicarbonate, sodium bisulphate, sodium carbonate, sodium chloride, sodium hypophosphite, sodium malonate, sodium maleate, sodium meta-bisulphite, sodium sulphate, sodium sulphite, trilithium citrate, triammonium citrate, trisodium citrate, tripotassium citrate, zinc chloride, and/or zinc sulphate.
  • salts including, but not limited to, ammonium sulphate, disodium hydrogen phosphite, dipotassium phosphate, lithium sulphate, monosodium phosphate, potassium carbonate, potassium chloride, potassium sulphate, sodium bicarbonate, sodium bisulphate, sodium
  • a coagulation bath solution of Step (d) contains one or more salts including, but not limited to, disodium malate.
  • sodium malate, and disodium malate are used interchangeably.
  • the concentration of each salt within a coagulation bath solution of Step (d) is within the range of 0.01 % (w/v) to 40 % (w/v); preferably, within the range of 0.1 % (w/v) to 10 % (w/v) more preferably, within the range of 0.5 % (w/v) to 5 % (w/v); and most preferably, at 1 % (w/v), relative to the volume of the solution.
  • the concentration of each salt within a coagulation bath solution of Step (d) is within the range of 0.01 % (w/v) to 0.1 % (w/v), 0.1 % (w/v) to 1 % (w/v), 1 % (w/v) to 5 % (w/v), 5 % (w/v) to 10 % (w/v), 10 % (w/v) to 20 % (w/v), and/or % (w/v) to 40 % (w/v) relative to the volume of the solution.
  • the temperature of a coagulation bath solution of Step (d) is room temperature, or at temperatures that are either higher or lower than room temperature; preferably, in the range of 5 °C to 95 °C; more preferably, in the range of 10 °C to 75 °C; even more preferably, in the range of 15 °C to 65 °C; yet even more preferably, in the range of 20 °C to 60 °C; and most preferably, in the range of 20 °C to 35 °C.
  • the temperature of a coagulation bath solution of Step (d) is within the range of 5 °C to 10 °C, 10 °C to 20 °C, 20 °C to 30 °C, 30 °C to 50 °C, 50 °C to 75 °C, or 75 °C to 95 °C.
  • a coagulation bath solution of Step (d) comprises water, which also contains sodium citrate 25 % (w/v) and sodium hypophosphite 12.5 % (w/v), relative to the volume of the coagulation bath solution, and is at a pH within the range of 7.01 to 9.99 at room temperature.
  • a coagulation bath solution of Step (d) comprises water which also contains sodium malate and is at a pH within the range of 7.01 to 9.99 at room temperature.
  • a coagulation bath solution of Step (d) does not have more than 5 % (w/v) polypeptides prior to the immersion of the extruded or spun fibres.
  • a coagulation bath solution of Step (d) does not comprise more than 1 % (w/v) polypeptides prior to the immersion of the extruded or spun hollow fibres.
  • a suitable coagulation bath solution may comprise a solvent, or multiple solvents, together with one or more polycarboxylic acid salt crosslinking agent(s) in a mixture, that may also include a mixture of catalysts and/or salts, such as sodium hypophosphite.
  • the coagulation bath solution may be formulated to facilitate the polycarboxylic acid derived ester, thioester or amide covalent bond crosslink mediated gelation, precipitation of the covalently- crosslinked hollow fibres. Consequently, this may facilitate the formation of the extruded or spun hollow fibres, comprising covalent ester, thioester and/or amide crosslinked polypeptides.
  • the fraction of the polypeptides in the extruded or spun hollow fibres which are covalently-crosslinked is at least 10 %, preferably, at least 20 % more preferably, at least 40 %, even more preferably, at least 60 % and most preferably, at least 80 %.
  • the fraction of the polypeptides in the extruded or spun hollow fibres which are covalently-crosslinked ranges from 10 % to 30 %, 20 % to 30 %, 30 % to 40 %, 40 % to 50 %, 50 to 60 %, 60 to 70 %, 70 to 80 %, 80 to 90 % or 90 % to 99.99 %.
  • a covalently-crosslinked sample is run through an SDS-PAGE gel alongside a non-crosslinked sample and a channel of proteins with known masses.
  • the theoretical mass of emergent bands in the crosslinked sample and non-crosslinked sample channels may be calculated by comparing the distance of the bands along the channels relative to that of the bands in the channel with proteins of known mass. By evaluating the relative masses of the bands in the crosslinked and non-cross linked sample channels, the degree of crosslinking associated with each band may be calculated.
  • Structural changes associated with crosslinking may be evaluated by the change in band intensities associated with various functional groups as determined by FTIR or Raman spectroscopy.
  • Step (d) comprises treating the polypeptides of the hollow fibres produced in Step (c) in a coagulation bath filled with an aqueous coagulation bath solution, comprising sodium citrate, at a pH between 8.0 and 8.5, room temperature, and a pressure between 100825 Pa to 101825 Pa, for a duration of 1 hour.
  • an aqueous coagulation bath solution comprising sodium citrate, at a pH between 8.0 and 8.5, room temperature, and a pressure between 100825 Pa to 101825 Pa, for a duration of 1 hour.
  • Step (d) comprises treating the polypeptides of the hollow fibres produced in Step (c) in a coagulation bath filled with an aqueous coagulation bath solution, comprising sodium succinate and sodium hypophosphite, at a pH between 8.0 and 8.5, room temperature, and a pressure between 100825 Pa to 101825 Pa, for 1 hour and 30 minutes.
  • an aqueous coagulation bath solution comprising sodium succinate and sodium hypophosphite
  • Step (d) comprises treating the polypeptides of the hollow fibres produced in Step (c) in a coagulation bath filled with an aqueous coagulation bath solution, comprising sodium malate and sodium hypophosphite, at a pH between 8.0 and 8.5, room temperature, and a pressure between 100825 Pa to 101825 Pa, for a duration of 30 minutes.
  • an aqueous coagulation bath solution comprising sodium malate and sodium hypophosphite
  • Step (d) comprises treating the polypeptides of the hollow fibres produced in Step (c) in a coagulation bath filled with an aqueous coagulation bath solution, comprising sodium malate, at a pH between 8.0 and 8.5, room temperature, and a pressure between 100825 Pa to 101825 Pa, for a duration of 30 minutes.
  • an aqueous coagulation bath solution comprising sodium malate
  • Step (d) comprises treating the polypeptides of the hollow fibres produced in Step (c) in a coagulation bath filled with an aqueous coagulation bath solution, comprising sodium citrate and sodium hypophosphite, at a pH between 8.0 and 8.5, room temperature, and a pressure between 100825 Pa to 101825 Pa, for a duration of 1 hour.
  • an aqueous coagulation bath solution comprising sodium citrate and sodium hypophosphite
  • the coagulation bath of any embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • the polycarboxylic acid crosslinking reagent of any embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • the covalently-crosslinked polypeptides of any embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • Step (e) may comprise treating the covalently-crosslinked, semi-permeable, porous hollow fibres, produced in Step (d), with at least one post-production modification process.
  • Step (e) comprises treating the covalently-crosslinked, semi-permeable, porous hollow fibres, produced in Step (d), with at least one post-production modification process.
  • the covalently-crosslinked, semi-permeable, porous hollow fibres may be further treated as part of their production, or as a post-production modification, in order to potentially enhance one or more of the mechanical properties, fluid transport, permeability and water stability of the hollow fibres.
  • the covalently-crosslinked, semi-permeable, porous hollow fibres are further treated as part of their production, or as a post-production modification, to enhance one or more of the mechanical properties, fluid transport, permeability and water stability of the hollow fibres.
  • Step (e.i) may comprise treating the covalently-crosslinked, semi-permeable, porous hollow fibres with an organic solvent to increase the relative abundance of beta-sheets in the secondary structure of the polypeptides.
  • Step (e.i) comprises treating the covalently-crosslinked, semi- permeable, porous hollow fibres with an organic solvent to increase the relative abundance of beta-sheets in the secondary structure of the polypeptides.
  • the covalently-crosslinked, semi-permeable, porous hollow fibres may be treated with organic solvents to dehydrate them and alter the secondary macromolecular structures of the constituent polypeptides.
  • the covalently-crosslinked, semi-permeable, porous hollow fibres are treated with organic solvents to dehydrate them and alter the secondary macromolecular structures of the constituent polypeptides.
  • the aim of Step (e.i) is to potentially alter the secondary macromolecular structure of the polypeptides in the covalently-crosslinked, semi-permeable, porous hollow fibres, for the purpose of increasing the abundance of beta-sheets and beta-coils, relative to amorphous random coils and alpha-helices.
  • This change in the secondary structure may result in an increase in water stability of the hollow fibres, such that their mechanical properties may remain relatively unaltered following continuous submersion in an aqueous solution for 3 or more days (24) (25) (27; 28).
  • the alteration of the secondary macromolecular structure of the polypeptides in the covalently-crosslinked, semi-permeable, porous hollow fibres is achieved with organic solvents and, either through solution washes and/or vapour exposure.
  • the alteration of the secondary macromolecular structure of the polypeptides in the covalently-crosslinked, semi-permeable, porous hollow fibres is achieved with organic solvents in an aqueous solution.
  • the alteration of the secondary macromolecular structure of the polypeptides in the covalently-crosslinked, semi-permeable, porous hollow fibres is achieved with supercritical CO 2 .
  • the covalently-crosslinked, semi-permeable, porous hollow fibres are treated with one or more organic solvents that include, but are not limited to methanol, ethanol, propanol, iso-propanol, butanol, iso-butanol, dimethyl sulfoxide, dimethylformamide, dimethylacetamide, and/or mixtures thereof.
  • organic solvents include, but are not limited to methanol, ethanol, propanol, iso-propanol, butanol, iso-butanol, dimethyl sulfoxide, dimethylformamide, dimethylacetamide, and/or mixtures thereof.
  • the covalently-crosslinked, semi-permeable, porous hollow fibres are treated with one or more organic solvents that include acetone.
  • the covalently-crosslinked, semi-permeable, porous hollow fibres are treated with one or more polyol(s) and/or polymer(s) of polyols.
  • the alteration of the secondary macromolecular structure of the polypeptides in the covalently-crosslinked, semi-permeable, porous hollow fibres is achieved with polyol(s) and/or polymer(s) of polyols in an aqueous solution.
  • the alteration of the secondary macromolecular structure of the polypeptides in the covalently-crosslinked, semi-permeable, porous hollow fibres is achieved a solution comprising water, organic solvents, polyol(s) and/or polymer(s) of polyols.
  • the covalently-crosslinked, semi-permeable, porous hollow fibres are treated with one or more fluids including, but not limited to, supercritical CO 2 .
  • supercritical CO 2 is considered to behave akin to an organic solvent, above the critical point of CO 2 , and is referred to as such.
  • organic solvent is considered to include supercritical CO 2 .
  • the concentration of any singular component in the solvent mixture used in the formation of beta-sheets in the covalently-crosslinked, semi-permeable, porous hollow fibres is within the range of 0 % (w/v) to 100 % (w/v); preferably, within the range of 10 % (w/v) to 75 % (w/v); and most preferably, within the range of 20 % (w/v) to 60 % (w/v), relative to the total solvent mixture volume.
  • the concentration of any singular component in the solvent mixture used in the formation of beta-sheets in the covalently-crosslinked, semi-permeable, porous hollow fibres is within the range of 0 % (w/v) to 5 % (w/v), 5 % (w/v) to 10 % (w/v), 10 % (w/v) to 15 % (w/v), 15 % (w/v) to 20 % (w/v), 20 % (w/v) to 25 % (w/v), 25 % (w/v) to 30 % (w/v), 30 % (w/v) to 35 % (w/v), 35 % (w/v) to 40 % (w/v), 40 % (w/v) to 45 % (w/v), 45 % (w/v) to 50 % (w/v), 50 % (w/v) to 55 % (w/v), 55 % (w/v) to 60 % (w/v),
  • the treatment of the covalently-crosslinked, semi-permeable, porous hollow fibres with one or more organic solvents is carried out at a temperature that is within the range of 0 °C to 90 °C; preferably, within the range of 10 °C to 80 °C; more preferably, within the range of 20 °C to 70 °C; even more preferably, within the range of 30 °C to 60 °C; and most preferably, at room temperature.
  • the treatment of the covalently-crosslinked, semi-permeable, porous hollow fibres with one or more organic solvents is carried out at a temperature that is within the range of 10 °C to 20 °C, 20 °C to 30 °C, 30 °C to 40 °C, 40 °C to 50 °C, 50 °C to 60 °C, 60 °C to 70 °C, 70 °C to 80 °C, or 80 °C to 90 °C.
  • the treatment of the covalently-crosslinked, semi-permeable, porous hollow fibres with one or more solvent is carried out at a temperature that is within the range of 15 to 300 °C, preferably, within the range of 100 °C to 250 °C; more preferably, within the range of 130 °C to 200 °C; even more preferably, within the range of 150 °C to 180 °C; and most preferably, at 175 °C.
  • the treatment of the covalently-crosslinked, semi-permeable, porous hollow fibres with one or more solvent is carried out across a range of temperatures.
  • the treatment of the covalently-crosslinked, semi-permeable, porous hollow fibres with one or more organic solvents is carried out at atmospheric pressure, or at pressures that are either higher or lower than atmospheric pressure; preferably, in the range of 90000 Pa to 109000 Pa; more preferably, in the range of 96400 Pa to 108400 Pa; and most preferably, in the range of 100825 Pa to 101825 Pa.
  • the pressure used in the organic solvent wash step is in the range of 90000 Pa to 109000 Pa, 95000 Pa to 105000 Pa, or 100000 Pa to 102000 Pa.
  • the treatment of the covalently-crosslinked, semi-permeable, porous hollow fibres with one or more solvent is carried out at a pressure that is within the range of 0.1 MPa to 100 MPa; preferably, in the range of 7 MPa to 50 MPa; and more preferably, in the range of 10 MPa to 25 MPa.
  • the treatment of the covalently-crosslinked, semi-permeable, porous hollow fibres with one or more solvent is carried out in a pressure vessel. In some embodiments, the treatment of the covalently-crosslinked, semi-permeable, porous hollow fibres with one or more organic solvents is carried out over a duration that is within the range of 1 second to 10 days; preferably, within the range of 10 minutes and 3 hours; more preferably, within the range ranging from 40 and 90 minutes; and most preferably for 1 hour.
  • the treatment of the covalently-crosslinked, semi-permeable, porous hollow fibres with one or more organic solvents is carried out over a duration that is within the range of 10 minutes and 30 minutes, 30 minutes and 45 minutes, 45 minutes and 1 hour, 1 hour and 2 hours, 2 hours and 3 hours, 3 hours and 4 hours, 4 hours and 5 hours, 5 hours and 6 hours, 6 hours and 7 hours, 7 hours and 8 hours, 8 hours and 9 hours, 9 hours and 10 hours, 10 hours and 11 hours, 11 hours and 12 hours, 12 hours and 13 hours, 13 hours and
  • Changes in the secondary macromolecular structure of the polypeptides in the covalently- crosslinked hollow fibres may be determined by evaluating the changes in intensity of peaks associated with amorphous random coils, alpha-helices, beta-helices, beta-sheets and disordered structures. This type of analysis may be performed by a person of skill in the art using methods that include Fourier transform infrared (FTIR) spectroscopy, circular dichroism, wide-angle X-ray scattering (WAXS), and Raman spectroscopy.
  • FTIR Fourier transform infrared
  • WAXS wide-angle X-ray scattering
  • Raman spectroscopy Raman spectroscopy
  • Step (e.i) comprises treating the covalently- crosslinked, semi-permeable, porous hollow fibres produced in Step (d) in a liquid solvent bath comprising at least 40 % ethanol at room temperature and atmospheric pressure for one hour.
  • a coagulation bath solution of Step (d) comprises organic solvents, such that Step (d) is effectively combined with Step (e.i).
  • betasheet formation occurs concurrent to the coagulation, solidification, and cross-linking of the polypeptides.
  • the organic solvents used in of any the embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • Step (e.ii) may comprise annealing the covalently-crosslinked, semi-permeable, porous hollow fibres.
  • the annealing process typically allow crystalline structures to rearrange into more stable forms which may be used to decrease internal stresses in the covalently-crosslinked, semi-permeable, porous hollow fibres. More specifically, the annealing process may allow the beta-sheets formed in Step (e.i) to become the stable micro-structure state of the hollow fibres. Consequently, the beta-sheet micro-structure may remain intact upon rehydration of the hollow fibres and prevent the hydrolysis of the ester crosslinks formed in Step (d).
  • the consecutive covalent ester crosslinking, beta-sheet formation and annealing steps may grant the hollow fibres longterm water stability, enabling their use for long-term cell culture, as necessary in the production of cultivated meat products.
  • the annealing process allows crystalline structures to rearrange into more stable forms to decrease internal stresses in the covalently-crosslinked, semi-permeable, porous hollow fibres. More specifically, the annealing process results in the beta-sheets formed in Step (e.i) to become the stable micro-structure state of the hollow fibres. Consequently, the beta-sheet micro-structure remains intact upon rehydration of the hollow fibres and prevents the hydrolysis of the ester crosslinks formed in Step (d).
  • the consecutive covalent ester crosslinking, beta-sheet formation and annealing steps grant the hollow fibres long-term water stability, enabling their use for long-term cell culture, as necessary in the production of cultivated meat products.
  • the treated covalently-crosslinked, semi-permeable, porous hollow fibres may be partially or completely dried prior to annealing, in a process herein referred to as “pre-drying”.
  • the treated covalently-crosslinked, semi-permeable, porous hollow fibres are partially dried prior to annealing, such that some water still remains within the microstructure of the hollow fibres.
  • the treated covalently-crosslinked, semi-permeable, porous hollow fibres are completely dried prior to annealing, such that, ostensibly, all of the water is removed from the microstructure of the hollow fibres.
  • the treated covalently-crosslinked, semi-permeable, porous hollow fibres are not dried prior to annealing.
  • the pre-drying process is carried out at temperatures that range from 5 to 90 °C; preferably, within the range of 15 °C to 80 °C; more preferably, within the range of 40 °C to 70 °C; and most preferably, within the range of 50 °C to 70 °C.
  • the pre-drying process is carried out at temperatures that range from 5 °C to 10 °C, 10 °C to 15 °C, 15 °C to 20 °C, 20 °C to 25 °C, 25 °C to 30 °C, 30 °C to 35 °C, 35 °C to 40 °C, 40 °C to 45 °C, 45 °C to 50 °C, 50°C to 55 °C, 55 °C to 60 °C, 60 °C to 65 °C, 65 °C to 70 °C, 70 °C to 75 °C, 75 °C to 80 °C, 80 °C to 85 °C, and/or 85 °C to 90 °C.
  • the treated covalently-crosslinked, semi-permeable, porous hollow fibres are dried prior to annealing with freeze-drying.
  • the treated covalently-crosslinked, semi-permeable, porous hollow fibres are not dried prior to annealing with freeze-drying.
  • the pre-drying process is carried out at temperatures that range from 5 to -90 °C; preferably, within the range of -15 °C to -90 °C; more preferably, within the range of -40 °C to -90 °C; even more preferably, within the range of -60 °C to -90 °C; and most preferably, within the range of -80 °C to -86 °C.
  • the pre-drying process is carried out at temperatures that range from 5 °C to 0 °C, 0 °C to -10 °C, -10 °C to -20 °C, -20 °C to -30 °C, -30 °C to -40 °C, -40 °C to -50 °C, -50 °C to -60 °C, -60 °C to -70 °C, -70 °C to -80 °C, -80 °C to -86 °C, or -80 °C to -90 °C.
  • the treated covalently-crosslinked, semi-permeable, porous hollow fibres are dried under conditions below the Triple point of water.
  • the pre-drying process is carried out over a duration within the range of 0 minutes to 7 days; preferably, 20 minutes to 5 days; more preferably, 40 minutes to 3 days; even more preferably, 1 hour to 2 days; and most preferably, within the range of 2 hours to 1 day.
  • the pre-drying process is carried out over a duration within the range 0 minutes to 5 minutes, 5 minutes to 10 minutes, 10 minutes to 15 minutes, 15 minutes to 20 minutes, 20 minutes to 25 minutes, 25 minutes to 30 minutes, 30 minutes to 35 minutes, 35 minutes to 40 minutes, 40 minutes to 45 minutes, 45 minutes to 50 minutes, 50 minutes to 55 minutes, 55 minutes to 1 hour, 1 hour to 2 hours, 2 hours to 3 hours, 3 hours to 4 hours, 4 hours to 5 hours, 5 hours to 6 hours, 6 hours to 12 hours, 12 hours to 18 hours, 18 hours to 24 hours, 24 hours to 30 hours, 30 hours to 36 hours, 36 hours to 42 hours, 42 hours to 48 hours, 48 hours to 54 hours, 54 hours to 60 hours, 60 hours to 66 hours, 66 hours to 3 days, 3 days to 4 days, 4 days to 5 days, 5 days to 6 days, or 6 days to 7 days.
  • the pre-drying process may be performed with convection, an infra-red heat source, and/or on a heated surface.
  • the pre-drying process is performed with convection, radiation, microwave, an infra-red heat source, and/or on a heated surface.
  • the annealing step may be performed in different atmospheres, liquids and/or mixtures thereof.
  • the annealing step is performed in different atmospheres, liquids and/or mixtures thereof.
  • the atmospheres used in the annealing step may include, but are not limited to, air, carbon dioxide, steam, inert gases (such as nitrogen, helium, neon, argon, and xenon), vapours (such as ethanol, plant oils, water) and/or a mixture thereof.
  • the atmospheres used in the annealing step include, but are not limited to, air, carbon dioxide, steam, inert gases (such as nitrogen, helium, neon, argon, and xenon), vapours (such as ethanol, plant oils, water) and/or a mixture thereof.
  • inert gases such as nitrogen, helium, neon, argon, and xenon
  • vapours such as ethanol, plant oils, water
  • the liquids used in the annealing step may include, but are not limited to, ethanol, plant oils, and/or a mixture thereof.
  • the fluids used in the annealing step may include, but are not limited to, alcohols such as, methanol, ethanol, propanol, iso-propanol, butanol, and/or a mixture thereof.
  • the fluids used in the annealing step may include, but are not limited to, supercritical CO2.
  • the liquids used in the annealing step include, but are not limited to, ethanol, plant oils, and/or a mixture thereof.
  • the fluids used in the annealing step include, but are not limited to, alcohols such as, methanol, ethanol, propanol, iso-propanol, butanol, and/or a mixture thereof.
  • the fluids used in the annealing step include, but are not limited to, supercritical CO2.
  • the liquids used in the annealing step do not include polyols.
  • the hollow fibres are annealed by baking them in a convection oven, steaming them in an autoclave or pressure cooker, and/or submerging them in a hot oil bath.
  • the annealing step is conducted in an atmosphere of air. In some embodiments, the annealing step is carried out at a temperature that is within the range of 50 °C to 180 °C; preferably, within the range of 75 °C to 160 °C; more preferably, within the range of 110 °C to 140 °C; and most preferably, within the range of 120 °C to 130 °C.
  • the annealing step is carried out at temperatures that range from 50 °C to 80 °C, 80 °C to 100 °C, 100 °C to 120 °C, 120 °C to 140 °C, 140 °C to 160 °C, or 160 °C to 180 °C.
  • the annealing step is carried out at a temperature that is within the range of 180 °C to 300 °C; preferably, within the range of 200 °C to 280 °C; more preferably, within the range of 220 °C to 260 °C; and most preferably, within the range of 240 °C to 250 °C.
  • the annealing step is carried out at temperatures that range from 180 °C to 200 °C, 200 °C to 220 °C, 220 °C to 240 °C, 240 °C to 260 °C, 260 °C to 280 °C, or 280 °C to 300 °C.
  • the annealing step is performed over a duration that is within the range of 30 minutes to 6 hours; preferably, within the range of 10 minutes and 4 hours; more preferably, within the range of 30 minutes and 3 hours, even more preferably, within the range of 45 hours and 2 hours; and most preferably, within the range of 1 hours and 1.5 hours.
  • the annealing step is performed over a duration that is within the range of 5 minutes to 15 minutes, 15 minutes to 30 minutes, 30 minutes to 45 minutes, 45 minutes to 1 hour, 1 hour to 1.25 hours, 1 .25 hours to 1 .5 hours, 1 .5 hours to 1 .75 hours, 1.75 hours to 2 hours, 2 hours to 2.25 hours, 2.25 hours to 2.5 hours, 2.5 hours to 2.75 hours, 2.75 hours to 3 hours, 3 hours to 3.25 hours, 3.25 hours to 3.5 hours, 3.5 hours to 3.75 hours, 3.75 hours to 4 hours, 4 hours to 4.25 hours, 4.25 hours to 4.5 hours, 4.5 hours to 4.75 hours, 4.75 hours to 5 hours, 5 hours to 5.25 hours, 5.25 hours to 5.5 hours, 5.5 hours to 5.75 hours, and/or
  • the annealed covalently-crosslinked, semi-permeable, porous hollow fibres may be rehydrated in water and/or an aqueous solution, in a process herein referred to as rehydration.
  • the annealed covalently-crosslinked, semi-permeable, porous hollow fibres are rehydrated in water and/or an aqueous solution.
  • the annealed covalently-crosslinked, semi-permeable, porous hollow fibres are not rehydrated in water and/or an aqueous solution.
  • components in the aqueous solution for rehydration include, but are not limited to, water, alcohols, polyols, acids, alkalis, salts and/or combinations thereof.
  • the aqueous rehydration solution comprises polyols.
  • Step (e.ii) comprises annealing the covalently-crosslinked, semi-permeable, porous hollow fibres at 130 °C in air for two hours.
  • Step (e.ii) comprises drying the covalently- crosslinked, semi-permeable, porous hollow fibres at 60 °C with convection for 2 hours, followed by annealing at 130 °C in air for 1 hour, and subsequent rehydration in an aqueous solution containing 20 % glycerol (v/v).
  • the pre-drying conditions used in any of the embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • the annealing conditions used in any of the embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • the process by which Prokitein is produced comprises protein annealing.
  • Prokitein is annealed as to alter and/or tailor its material properties.
  • Prokitein is annealed as to alter and/or tailor its Youngs’ modulus.
  • Prokitein is annealed as to alter and/or tailor its ultimate tensile stress. In some other embodiments, Prokitein is annealed as to alter and/or tailor its ultimate tensile strain.
  • Step (e.iii) may comprise treating the covalently-crosslinked, semi-permeable, porous hollow fibres with one or more solvents to remove void inclusion elements in the hollow fibres, and may impart porosity.
  • Step (e.iii) comprises treating the covalently-crosslinked, semi- permeable, porous hollow fibres with one or more solvents to remove void inclusion elements in the hollow fibres, thereby, imparting porosity.
  • solvents which are used to remove void inclusion elements include aqueous solutions and/or organic solutions.
  • supercritical fluids are used to remove void inclusion elements.
  • supercritical CO 2 is used to remove void inclusion elements.
  • aqueous solutions comprise, water, acids, alkalis and/or salt solutions.
  • one of the solvents is water.
  • one or more of the solvents is an aqueous solution comprising acids that include at least one of oxalic acid, malic acid, succinic acid, adipic acid, tartaric acid, citric acid, malonic acid, acetic acid, or formic acid; preferably citric acid and/or malic acid.
  • the plurality of covalently-crosslinked semi-permeable, porous hollow fibres are treated with an aqueous acid solution, such as citric acid.
  • Hydrogen ions react with the calcium carbonate embedded in the walls of the hollow fibres, to form calcium salts and carbon dioxide.
  • the carbon dioxide is released and the salts dissolve, voids are created in the walls of the hollow fibres, in the spaces previously occupied by calcium carbonate; thereby, increasing the void fraction in the hollow fibres.
  • the organic solution comprises at least one of ethanol, propanol, isopropanol, butanol, iso-butanol, dimethyl sulfoxide, dimethylformamide, and/or dimethylacetamide; preferably ethanol.
  • the organic solution comprises acetone.
  • the organic solution comprises polyol(s) and/or polymer(s) of polyols.
  • aqueous solutions and organic solutions may be used in combination to form a solvent mixture.
  • the plurality of hollow fibres is exposed to an organic solvent, such as ethanol.
  • an organic solvent such as ethanol.
  • voids are created in the walls of the hollow fibres in the spaces previously occupied by the oil droplets; thereby, imparting porosity onto the hollow fibres.
  • the concentration of any singular component in the solvent mixture used to remove the void inclusion elements from the covalently-crosslinked, semi-permeable, porous hollow fibres is within the range of 0 % (w/v) to 100 % (w/v); preferably, within the range of 10 % (w/v) to 75 % (w/v); and most preferably, within the range of 20 % (w/v) to 60 % (w/v), relative to the total solvent mixture volume.
  • the concentration of any singular component in the solvent mixture used to remove the void inclusion elements from the covalently-crosslinked, semi-permeable, porous hollow fibres is within the range of 0 % (w/v) to 5 % (w/v), 5 % (w/v) to 10 % (w/v), 10 % (w/v) to 15 % (w/v), 15 % (w/v) to 20 % (w/v), 20 % (w/v) to 25 % (w/v), 25 % (w/v) to 30 % (w/v), 30 % (w/v) to 35 % (w/v), 35 % (w/v) to 40 % (w/v), 40 % (w/v) to 45 % (w/v), 45 % (w/v) to 50 % (w/v), 50 % (w/v) to 55 % (w/v), 55 % (w/v) to 60 % (w/v), 60
  • Step (e.iii) comprises subjecting the covalently-crosslinked, semi-permeable, porous hollow fibres to pyrolysis-induced pore formation.
  • pyrolysis-induced pore formation refers to a process of thermal decomposition of one or more void inclusion elements in the wall of the hollow fibre to remove said void inclusion elements, thereby, imparting porosity.
  • Step (e.iii) comprises treating the covalently- crosslinked, semi-permeable, porous hollow fibres, comprising powdered calcium carbonate, with 25 % (w/v) citric acid in an aqueous solvent at room temperature for 30 minutes.
  • Step (e.iii) comprises treating the covalently- crosslinked, semi-permeable, porous hollow fibres, comprising lipids, with a 40 % ethanol solution at room temperature for 30 minutes.
  • Step (e.iii) comprises treating the covalently- crosslinked, semi-permeable, porous hollow fibres, comprising lipids, with iso-propanol at room temperature for 30 minutes.
  • Step (e.iii) comprises treating the covalently- crosslinked, semi-permeable, porous hollow fibres, comprising lipids, with supercritical CO 2 .
  • one or more void inclusion elements may be removed from the hollow fibres in processing steps that are separate to Step (e.iii), including, but are not limited to, Step (d), Step (e.i), and/or Step (e.iv).
  • Step (d) water-soluble void inclusion elements could dissolve in a coagulation bath solution of Step (d).
  • Step (d) a coagulation bath solution of Step (d)
  • alcohol-soluble void inclusion elements could dissolve in a coagulation bath solution of Step (d) comprising aqueous solvents and one or more alcohols.
  • one or more void inclusion elements are removed from the hollow fibres in processing steps that are separate to Step (e.iii), including, but are not limited to, Step (d), Step (e.i), and/or Step (e.iv).
  • the solvent used to remove void inclusion elements in any of the embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • Step (e.iv) may comprise washing the covalently-crosslinked, semi-permeable, porous hollow fibres with one or more acid(s), alkali(s) and/or buffer solution(s) to potentially decrease at least one of, the Young’s modulus, ultimate tensile strength and/or ultimate tensile strain of the hollow fibres.
  • Step (e.iv) comprises washing the covalently-crosslinked, semi- permeable, porous hollow fibres with one or more acid(s), alkali(s) and/or buffer solution(s) to decrease at least one of, the Young’s modulus, ultimate tensile strength and/or ultimate tensile strain of the hollow fibres.
  • the acids used in Step (e.iv) include, but are not limited to, ascorbic acid, acetic acid, adipic acid, citric acid, formic acid, hydrochloric acid, lactic acid, malic acid, malonic acid, oxalic acid, succinic acid, sulphuric acid and/or tartaric acid.
  • the alkalis used in Step (e.iv) include, but are not limited to, sodium hydroxide, potassium hydroxide, calcium hydroxide, magnesium hydroxide and/or sodium carbonate.
  • the covalently-crosslinked, semi-permeable, porous hollow fibres are washed in Step (e.iv) with a buffer solution, buffered to a pH of 10 or higher.
  • the covalently-crosslinked, semi-permeable, porous hollow fibres are washed in Step (e.iv) with a sodium carbonate buffer solution, buffered to a pH of 10 or higher.
  • Step (e.iv) lasts for a duration that is within the range of, 10 minutes to 3 hours; preferably, 15 minutes and 2 hours; and most preferably, from 30 minutes to 1 hour. In some other embodiments, Step (e.iv) lasts for a duration that is within the range of 10 minutes and 15 minutes, 15 minutes and 30 minutes, 30 minutes and 45 minutes, 45 minutes and 1 hour, 1 hour and 2 hours, or 2 hours and 3 hours.
  • Step (e.iv) is performed at a temperature that is within the range of 10 °C to 80 °C; preferably, within the range of 20 °C and 60 °C; more preferably, within the range of 30 °C and 50 °C; and most preferably, at room temperature.
  • Step (e.iv) is performed at a temperature that is within the range of 10 °C to 20 °C, 20 °C to 30 °C, 30 °C to 40 °C, 40 °C to 50 °C, 50 °C to 60 °C, 60 °C to 70 °C, or 70 °C to 80 °C.
  • Step (e.iv) comprises washing the covalently- crosslinked, semi-permeable, porous hollow fibres with an aqueous solution of sodium carbonate buffer with a pH of 11.0 at 37 °C for 60 minutes.
  • Step (e.v) may comprise coating the covalently-crosslinked semi-permeable, porous hollow fibres.
  • Step (e.v) comprises coating the covalently-crosslinked semi-permeable, porous hollow fibres.
  • Coatings are widely used in conjunction with traditional cell culture techniques to aid cell attachment and/or proliferation.
  • the coatings which may be used include, but are not limited to, protein complexes, cell attachment peptide sequences (CAPs), and/or growth factors (30; 31).
  • Protein complexes may be used to enhance cell attachment to covalently-crosslinked, semi- permeable, porous hollow fibres. These, include, but are not limited to, collagen, gelatine, fibrinogen, fibronectin and laminin.
  • protein complexes are used to enhance cell attachment to covalently- crosslinked, semi-permeable, porous hollow fibres. In some embodiments, protein complexes are not used to enhance cell attachment to covalently-crosslinked, semi-permeable, porous hollow fibres.
  • CAPs are short sequences of amino acids that embody the minimum motif required for cell attachment (30).
  • CAPs which may be used to enhance cell attachment to covalently-crosslinked, semi- permeable, porous hollow fibres include, but are not limited to, AELDVP (SEQ ID NO: 1), CGGNGEPRGDTYRAY (SEQ ID NO: 2), CFALRGDNP (SEQ ID NO: 3), CKKQRFRHRNRKG (SEQ ID NO: 4), CNYYSNS (SEQ ID NO: 5), CSVTCG (SEQ ID NO: 6), DGEA (SEQ ID NO: 7), ELVTDFPTDLPAT (SEQ ID NO: 8), FHRRIKA (SEQ ID NO: 9), FQGVLQNVRFVF (SEQ ID NO: 10), GACRGDCLGA (cyclic) (SEQ ID NO: 11), GFOGER (SEQ ID NO: 12), GFRGDGQ (SEQ ID NO: 13), GRGDS (SEQ ID NO: 14), GRGDAC (SEQ ID NO: 15), GTFALRGDNGQ (SEQ ID NO: 16), IDAPS
  • CAPs are used to enhance cell attachment to covalently-crosslinked, semi-permeable, porous hollow fibres.
  • CAPs which are used to enhance cell attachment to covalently- crosslinked, semi-permeable, porous hollow fibres include, but are not limited to, AELDVP (SEQ ID NO: 1), CGGNGEPRGDTYRAY (SEQ ID NO: 2), CFALRGDNP (SEQ ID NO: 3), CKKQRFRHRNRKG (SEQ ID NO: 4), CNYYSNS (SEQ ID NO: 5), CSVTCG (SEQ ID NO: 6), DGEA (SEQ ID NO: 7), ELVTDFPTDLPAT (SEQ ID NO: 8), FHRRIKA (SEQ ID NO: 9), FQGVLQNVRFVF (SEQ ID NO: 10), GACRGDCLGA (cyclic) (SEQ ID NO: 11), GFOGER (SEQ ID NO: 12), GFRGDGQ (SEQ ID NO: 13), GRGDS (SEQ ID NO: 14), GRGDAC (SEQ ID NO: 15), GTFALRGDNGQ (SEQ ID NO: 16
  • CAPs are not used to enhance cell attachment to covalently-crosslinked, semi-permeable, porous hollow fibres.
  • Growth factors may be used to enhance cell attachment to covalently-crosslinked, semi- permeable, porous hollow fibres. These include, but are not limited to, basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), insulin-like growth factor (IGF), epidermal growth factor (EGF), and/or vascular endothelial growth factor (VEGF).
  • bFGF basic fibroblast growth factor
  • HGF hepatocyte growth factor
  • IGF insulin-like growth factor
  • EGF epidermal growth factor
  • VEGF vascular endothelial growth factor
  • growth factors are used to enhance cell attachment to covalently- crosslinked, semi-permeable, porous hollow fibres.
  • growth factors are used to enhance cell attachment to covalently- crosslinked, semi-permeable, porous hollow fibres.
  • growth factors include, but are not limited to, basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), insulin-like growth factor (IGF), epidermal growth factor (EGF), and/or vascular endothelial growth factor (VEGF).
  • bFGF basic fibroblast growth factor
  • HGF hepatocyte growth factor
  • IGF insulin-like growth factor
  • EGF epidermal growth factor
  • VEGF vascular endothelial growth factor
  • growth factors are not used to enhance cell attachment to covalently- crosslinked, semi-permeable, porous hollow fibres.
  • protein complexes, CAPs and/or growth factors are used to enhance cell attachment to covalently-crosslinked, semi-permeable, porous hollow fibres.
  • Chemical methods, such as grafting may be used to coat the surface of the covalently- crosslinked, semi-permeable, porous hollow fibres with protein complexes, CAPS and/or growth factors to enhance cell attachment to the hollow fibres.
  • chemical methods are used to coat the surface of the covalently- crosslinked, semi-permeable, porous hollow fibres with protein complexes, CAPS and/or growth factors to enhance cell attachment to the hollow fibres.
  • chemical methods are not used to coat the surface of the covalently- crosslinked, semi-permeable, porous hollow fibres with protein complexes CAPS and/or growth factors to enhance cell attachment to the hollow fibres.
  • Step (e.v) comprises leaving the covalently- crosslinked, semi-permeable, porous hollow fibres uncoated.
  • Step (e.vi) may comprise modifying the surface topography of the covalently-crosslinked, semi- permeable, porous hollow fibres to aid cell attachment and/or cell alignment.
  • Step (e.vi) comprises modifying the surface topography of the covalently-crosslinked, semi-permeable, porous hollow fibres to aid cell attachment and/or cell alignment.
  • Step (e.vi) may comprise stretching the covalently-crosslinked, semi-permeable, porous hollow fibres to create tears in the microstructure, forming porous structures.
  • Step (e.vi) comprises stretching the covalently-crosslinked, semi- permeable, porous hollow fibres to create tears in the microstructure, forming porous structures.
  • the surface topography of the outer wall of the covalently-crosslinked, semi-permeable, porous hollow fibres may be modified. In some embodiments, the surface topography of the outer wall of the covalently-crosslinked, semi-permeable, porous hollow fibres is modified.
  • the surface topography of the outer wall of the covalently-crosslinked, semi-permeable, porous hollow fibres is not modified.
  • Methods which may be used to modify the surface include, but are not limited to, physical methods such as plasma treatment, physical vapour deposition, ultra-sonification and mechanical etching.
  • methods which are used to modify the surface include, but are not limited to, physical methods such as plasma treatment, physical vapour deposition, ultra- sonification and mechanical etching.
  • Mechanical etching may be used to impart striations along the surface of the covalently- crosslinked, semi-permeable, porous hollow fibres.
  • mechanical etching is used to impart striations along the surface of the covalently-crosslinked, semi-permeable, porous hollow fibres.
  • the die or spinneret orifice geometry, used in Step (c), may be used to impart striations along the surface of the covalently-crosslinked, semi-permeable, porous hollow fibres to aid the alignment of cells along the longitudinal axis of the hollow fibres.
  • the die or spinneret orifice geometry, used in Step (c), is used to impart striations along the surface of the covalently-crosslinked, semi-permeable, porous hollow fibres to aid the alignment of cells along the longitudinal axis of the hollow fibres.
  • Step (e.vi) comprises leaving the surface topography of the covalently-crosslinked, semi-permeable, porous hollow fibres unmodified.
  • Step (e) may be performed in any combination and in any sequence. In some embodiments, Step (e.i) is performed.
  • Step (e.i) is not performed.
  • Step (e.ii) is performed.
  • Step (e.ii) is not performed.
  • Step (e.iii) is performed.
  • Step (e.iii) is not performed
  • Step (e.iv) is performed.
  • Step (e.iv) is not performed.
  • Step (e.v) is performed.
  • Step (e.v) is not performed.
  • Step (e.vi) is performed.
  • Step (e.vi) is not performed.
  • Step (e.i) and Step (e.ii) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii) and Step (e.iii) are performed sequentially in the order specified.
  • Step (e.i), Step (e.iii) and Step (e.ii) are performed sequentially in the order specified. In some embodiments, one or more of any of Step (e.i), to Step (e.vi) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii), Step (e.iii) and Step (e.iv) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii), Step (e.iii), Step (e.iv) and Step (e.v) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii), Step (e.iii), Step (e.iv), Step (e.v) and Step (e.vi) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii), Step (e.iv), Step (e.v) and Step (e.vi) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii), Step (e.iii), Step (e.v) and Step (e.vi) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii), Step (e.iii), Step (e.iv), and Step (e.vi) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii), Step (e.iii), Step (e.iv), and Step (e.v) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii) and Step (e.iii) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii), and Step (e.iv) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii), and Step (e.v) are performed sequentially in the order specified. In some embodiments, Step (e.i), Step (e.ii), and Step (e.vi) are performed sequentially in the order specified.
  • Step (e.i), Step (e.ii) and Step (e.iii) are performed within a single process step.
  • Step (f) may comprise drying the treated covalently-crosslinked, semi-permeable, porous hollow fibres, to produce dried, treated covalently-crosslinked, semi-permeable, porous hollow fibres, and subsequently storing them in a low moisture atmosphere.
  • Step (f) comprises drying the treated covalently-crosslinked, semi- permeable, porous hollow fibres, to produce dried, treated covalently-crosslinked, semi- permeable, porous hollow fibres, and subsequently storing them in a low moisture atmosphere.
  • the treated covalently-crosslinked, semi-permeable, porous hollow fibres are partially dried; and preferably completely dried.
  • the treated covalently-crosslinked, semi-permeable, porous hollow fibres are not dried.
  • the drying process is performed with convection, radiation, freeze- drying, a microwave source, an infra-red heat source, and/or on a heated surface.
  • Freeze-drying may aid in the formation of pores and/or modify the microstructure of the covalently-crosslinked, semi-permeable, porous hollow fibres.
  • freeze-drying modifies the porosity of the covalently-crosslinked, semi-permeable, porous hollow fibres.
  • freeze-drying is additionally used modify the porosity of the covalently- crosslinked, semi-permeable, porous hollow fibres. In some embodiments, freeze-drying is not used modify the porosity of the covalently- crosslinked, semi-permeable, porous hollow fibres.
  • freeze-drying is used modify the porosity of the covalently-crosslinked, semi-permeable, porous hollow fibres; preferably, additionally used; and most preferably, not used.
  • the covalently-crosslinked, semi-permeable, porous hollow fibres are partially or completely dried at temperatures that range from 5 to 90 °C; preferably, within the range of 15 °C to 80 °C; more preferably, within the range of 40 °C to 70 °C; and most preferably, within the range of 50 °C to 70 °C.
  • the covalently-crosslinked, semi-permeable, porous hollow fibres are partially or completely dried at temperatures that range from 5 °C to 10 °C, 10 °C to 15 °C,
  • the covalently-crosslinked, semi-permeable, porous hollow fibres are dried using conditions applicable to the pre-drying of Step (e.ii).
  • the dried treated covalently-crosslinked, semi-permeable, porous hollow fibres may be stored in air, inert gases (such as nitrogen, helium, neon, argon, and xenon), CO 2 , under vacuum and/or in a PBS buffers solution, water, an aqueous solution, organic solution, and/or a mixture thereof.
  • inert gases such as nitrogen, helium, neon, argon, and xenon
  • CO 2 under vacuum and/or in a PBS buffers solution, water, an aqueous solution, organic solution, and/or a mixture thereof.
  • the dried treated covalently-crosslinked, semi-permeable, porous hollow fibres are stored under vacuum.
  • the dried treated covalently-crosslinked, semi-permeable, porous hollow fibres are stored in PBS buffer solution.
  • the dried treated covalently-crosslinked, semi-permeable, porous hollow fibres are stored in water and/or an aqueous solution.
  • Suitable components which are present within the aqueous solution could include, but are not limited to, water, alcohols, polyols, acids, alkalis, salts and/or combinations thereof.
  • the aqueous solution used to store the dried treated covalently-crosslinked, semi-permeable, porous hollow fibres comprises polyols.
  • the dried treated covalently-crosslinked, semi-permeable, porous hollow fibres may be stored in an organic solvent.
  • the dried treated covalently-crosslinked, semi-permeable, porous hollow fibres may be stored in a mixture of organic and aqueous solvents, such as, but not limited to, water and ethanol.
  • the dried treated covalently-crosslinked, semi-permeable, porous hollow fibres may be stored in refrigerated conditions, at temperatures between 8 and 0 °C.
  • the dried treated covalently-crosslinked, semi-permeable, porous hollow fibres may be stored in frozen conditions, at temperatures between 0 and -90 °C.
  • Step (f) comprises partially drying the treated covalently- crosslinked, semi-permeable, porous hollow fibres through exposure to heat, to produce partially dried, treated covalently-crosslinked, semi-permeable, porous hollow fibres, and subsequently storing them in air.
  • Step (f) comprises partially drying the treated covalently- crosslinked, semi-permeable, porous hollow fibres through exposure to heat, to produce partially dried, treated covalently-crosslinked, semi-permeable, porous hollow fibres, and subsequently storing them in an aqueous 40 % ethanol (v/v) solution.
  • Step (f) comprises partially drying the treated covalently- crosslinked, semi-permeable, porous hollow fibres through exposure to heat, to produce partially dried, treated covalently-crosslinked, semi-permeable, porous hollow fibres, and subsequently rehydrating them in an aqueous 20 % glycerol solution, and then storing them in air.
  • Step (f) comprises partially drying the treated covalently- crosslinked, semi-permeable, porous hollow fibres through exposure to heat, to produce partially dried, treated covalently-crosslinked, semi-permeable, porous hollow fibres, and subsequently storing them in an aqueous 20 % glycerol (v/v) solution.
  • process steps are carried out (one after the other) in the (a)-(f) order specified.
  • the process for the production of a plurality of semi-permeable, porous hollow fibres, comprising covalent ester, thioester and/or amide bond crosslinked polypeptides comprises sequentially carrying out Step (a), Step (b), Step (c), Step (d), Step (e.i), Step (e.ii) and Step (e.iii) in the order specified.
  • the process for the production of a plurality of semi-permeable, porous hollow fibres, comprising covalent ester, thioester and/or amide bond crosslinked polypeptides comprises sequentially carrying out Step (a), Step (b), Step (c), Step (d), Step (e.i), Step (e.iii) and Step (e.ii) in the order specified.
  • the Applicant also provides a plurality of semi-permeable, porous hollow fibres comprising covalent ester, thioester and/or amide crosslinked polypeptides, that are obtained or are obtainable by a process of the application.
  • drying conditions used in any of the embodiments of this disclosure can be used in the production of Prokitein and/or hollow fibres of this disclosure.
  • a semi-permeable, porous hollow fibre comprising one more polycarboxylic acid derived covalent ester, thioester and/or amide bond crosslinked polypeptides, wherein:
  • the external diameter of the hollow fibre is 50 - 6600 pM
  • the wall thickness of the hollow fibre is 20 - 800 pM
  • the lumen diameter of hollow fibre is 20 - 5000 pM; and (d) the pore volume of the hollow fibre is 1- 95 %.
  • a semi-permeable, porous hollow fibre comprising one more polycarboxylic acid derived covalent ester, thioester and/or amide bond crosslinked polypeptides, wherein:
  • the wall thickness of the hollow fibre is 20 - 800 pm;
  • the porosity of the hollow fibre is 1- 95 %.
  • the hollow fibres of this disclosure may comprise covalent ester, thioester and/or amide crosslinked polypeptides, and in general may each comprise a cylindrical tubular structure (e.g., a tube), having a wall, an outer surface, an inner surface, and a lumen.
  • a cylindrical tubular structure e.g., a tube
  • the lumen, inner wall and outer wall are labelled as 3006, 3005 and 3004, respectively.
  • the hollow fibres of this disclosure comprise covalent ester, thioester and/or amide crosslinked polypeptides, will each comprise a cylindrical tubular structure (e.g., a tube), having a wall, an outer surface, an inner surface, and a lumen.
  • a cylindrical tubular structure e.g., a tube
  • each hollow fibre of this disclosure may have a long axis, as labelled 3003 in Figure 3.
  • the cylindrical tubular structure of each hollow fibre of this disclosure has a long axis, as labelled 3003 in Figure 3.
  • each fibre of this disclosure may consist of a single tube that is preferably circular, although other shapes, such as, but not limited to, ovals, squares and rectangles may be used.
  • a schematic diagram of one example of a plurality of fibres in a bundle may be seen in Figure 4.
  • One example of a single fibre with a circular cylindrical tubular structure is labelled 4001 in Figure 4.
  • the cross-section of each fibre of this disclosure consists of a single tube that is preferably circular, although other shapes, such as, but not limited to, ovals, squares and rectangles can be used.
  • each fibre of this disclosure may comprise concentric shapes, such as, but not limited to, circles, ovals, squares and rectangles, or a combination thereof.
  • the cross-section of each fibre of this disclosure comprises concentric shapes, such as, but not limited to, circles, ovals, squares and rectangles, or a combination thereof.
  • the hollow fibres of this disclosure may be in the form of a tubular cylinder, preferably a circular tubular cylinder.
  • the hollow fibres of this disclosure are in the form of a tubular cylinder, preferably a circular tubular cylinder.
  • the hollow fibres of this disclosure may each have a lumen (i.e. , an orifice) that runs along their long axis, such that fluid may flow through the lumen.
  • a lumen i.e. , an orifice
  • the hollow fibres of this disclosure each have a lumen (i.e., an orifice) that runs along their long axis, such that fluid may flow through the lumen.
  • a lumen i.e., an orifice
  • the lumen of each fibre of this application is open at one or both ends.
  • each fibre of this application is open at both ends.
  • the hollow fibres of this disclosure may be of any desired length.
  • the hollow fibres of this disclosure are of any desired length.
  • the length of the hollow fibres is within the range of, but not limited to, 2 mm and 2 m; preferably, within the range of 1 cm and 1 m; and most preferably, within the range of 2.5 cm and 10 cm. In some embodiments, the length of the hollow fibres is within the range of, but not limited to, 2 mm and 5 m; preferably, within the range of 1 cm and 1 m; and most preferably, within the range of 2.5 cm and 10 cm.
  • the length of the hollow fibres of this disclosure are within the range of 2 mm and 1 cm, 1 cm and 5 cm, 5 cm and 10 cm, 10 cm and 50 cm, 50 cm and 1 m, or 1 m and 2 m.
  • the length of the hollow fibres of this disclosure are within the range of 2 mm and 1 cm, 1 cm and 5 cm, 5 cm and 10 cm, 10 cm and 50 cm, 50 cm and 1 m, 1 m and 2 m, 2 m and 3 m, 3 m and 4 m, or 4 m and 5 m.
  • the hollow fibres of this disclosure may be classified by their cross-sectional structure as being either: (i) symmetric (isotropic), in which the cross-sectional structure is constant; or (ii) asymmetric (non-symmetric) (anisotropic), in which the cross-sectional structure is not constant.
  • the hollow fibres of this disclosure are symmetric.
  • the hollow fibres of this disclosure are either symmetric, asymmetric, non-symmetric, or any combination thereof.
  • Each asymmetric fibre of this disclosure may comprise either: (i) a composite of two materials (asymmetric composite); or (ii) be of the same material (asymmetric integrally-skinned).
  • the hollow fibres of this disclosure consist solely of asymmetric composites, wherein each fibre comprise a composite of two materials.
  • the hollow fibres of this disclosure are all asymmetric integrally- skinned, wherein each fibre consists of the same material.
  • a plurality of fibres of this disclosure which comprises a mixture or at least one of:
  • the external diameter of a hollow fibre of this disclosure is within the range of 50 pm and 6600 pm; preferably, within the range or 100 pm to 1500 pm; and most preferably, within the range of 200 pm to 900 pm.
  • the external diameter of hollow fibres of this disclosure is within the range of range 50 pm to 100 pm, 100 pm to 200 pm, 200 pm to 300 pm, 300 pm to 400 pm, 400 pm to 500 pm, 500 pm to 600 pm, 600 pm to 700 pm, 700 pm to 800 pm, 800 pm to 900 pm, 900 pm to 1000 pm, 1000 pm to 1100 pm, 1100 pm to 1200 pm, 1200 pm to 1300 pm, 1300 pm to 1400 pm, 1400 pm to 1500 pm, 1500 pm to 1600 pm, 1600 pm to 1700 pm, 1700 pm to 1800 pm, 1800 pm to 1900 pm, 1900 pm to 2000 pm, 2000 pm to 2100 pm, 2100 pm to 2200 pm, 2200 pm to 2300 pm, 2300 pm to 2400 pm, 2400 pm to 2500 pm, 2500 pm to 2600 pm, 2600 pm to 2700 pm, 2700 pm to 2800 pm, 2800 pm to 2900 pm, 2900 pm to 3000 pm, 3000 pm, 3000 pm, 3000 pm to 3100 pm, 3100 pm to 3200 pm, 3200 pm
  • the wall thickness of each fibre of this application is within the range of 1 pm to 800 pm; preferably, within the range of 50 pm to 800 pm; more preferably, within the range of 70 pm to 500 pm; and most preferably, within the range of 75 pm to 150 pm.
  • the wall thickness of each fibre of this application is within the range of
  • the lumen diameter of hollow fibres of the disclosure is within the range of 20 m to 5000 pm; preferably, within the range of 50 pm to 1000 pm; more preferably, within the range of 100 pm to 850 pm; and most preferably, within the range of 150 pm to 500 pm.
  • the lumen diameter of a hollow fibre of the disclosure is within the range of from 20 pm to 50 pm, 50 pm to 100 pm, 100 pm to 200 pm, 200 pm to 500 pm, 500 pm to 1000 pm, 1000 pm to 2500 pm, or 2500 pm to 5000 pm.
  • Scanning electron microscopy may be used to produce images of a material with a wide range of magnifications from 10 to more than 500,000 times by scanning the surface of the material with a beam of electrons.
  • SEM image analysis can be used by a person of skill in the art to quantify various structural characteristics of the hollow fibres of this disclosure, including lumen diameter, wall thickness, surface pore diameter, and inner structure (32; 33).
  • the hollow fibres of this disclosure are semi-permeable.
  • the hollow fibres of this disclosure are semi-permeable, such that fluids and some solutes, including, but not limited to, nutrients, can pass through, but soluble growth factors, proteins and cells cannot.
  • the hollow fibres of this disclosure are semi-permeable, such that fluids and some solutes, including, but not limited to, nutrients, soluble growth factors and proteins can pass through, but cells cannot.
  • the hollow fibres of this disclosure are permeable, such that fluids, nutrients, soluble growth factors, proteins and cells can pass through.
  • Soluble growth factors that may be of particular interest in cellular agriculture include, but are not limited to, basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), insulin-like growth factor (IGF), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF) (34).
  • bFGF basic fibroblast growth factor
  • HGF hepatocyte growth factor
  • IGF insulin-like growth factor
  • EGF epidermal growth factor
  • VEGF vascular endothelial growth factor
  • Soluble growth factors may vary in size.
  • bFGF ranges between 18-34 kDa, depending on its form.
  • HGF is cleaved to form two active polypeptide chains, which are 69 kDa and 34 kDa in size.
  • IGF-1 is 7.6 kDa
  • EGF is 6 kDa
  • VEGF has 17 isoforms which range in size from 16-45 kDa.
  • the hollow fibres of this disclosure are semi-permeable such that fluids and some solutes smaller than 7 kDa may pass through, but some solutes larger than 7 kDa may not.
  • the hollow fibres of this disclosure are porous, semi-permeable such that fluids and solutes of sizes within the range 7 kDa to 12 kDa, 12 kDa to 17 kDa, 17 kDa to 22 kDa, 22 kDa to 27 kDa, 27 kDa to 32 kDa, 32 kDa to 37 kDa, 37 kDa to 42 kDa, 42 kDa to 47 kDa, 47 kDa to 52 kDa, 52 kDa to 57 kDa, 57 kDa to 62 kDa, 62 kDa to 67 kDa, 67 kDa to 72 kDa, 72 kDa to 77 kDa, or larger than 77 kDa can pass through.
  • the hollow fibres of this disclosure are semi-permeable such that fluids and solutes of sizes within the range 7 kDa to 12 kDa, 12 kDa to 17 kDa, 17 kDa to 22 kDa, 22 kDa to 27 kDa, 27 kDa to 32 kDa, 32 kDa to 37 kDa, 37 kDa to 42 kDa, 42 kDa to 47 kDa, 47 kDa to 52 kDa, 52 kDa to 57 kDa, 57 kDa to 62 kDa, 62 kDa to 67 kDa, 67 kDa to 72 kDa, 72 kDa to 77 kDa, or larger than 77 kDa can pass through.
  • Materials may be classified as being macroporous (> 50 nm), mesoporous (2 - 50 nm) or microporous ( ⁇ 2 nm) depending on the range of the pore sizes in the material.
  • Microporous materials may also be referred to as nanoporous.
  • a material is classified by the largest pore size present. For example, a porous material comprising pores which are smaller than 50 nm, as well as pores which are larger than 50 nm, would be classified as macroporous.
  • the hollow fibres of this disclosure are macroporous, mesoporous, or microporous.
  • the pore diameter of the pores in the walls of each hollow fibre of this disclosure is within the range of 1 nm to 100 pm; preferably, within the range of 5 nm to 15 pm; more preferably, within the range of 10 nm to 5 pm; even more preferably, within the range of 50 nm to 3 pm; and most preferably, within the range of 100 nm to 2 pm.
  • the pore diameter of each hollow fibre of this disclosure is within the range of 1 nm to 10 nm, 10 nm to 50 nm, 50 nm to 100 nm,100 nm to 500 nm, 500 nm to 1 pm, 1 pm to 5 pm, 5 pm to 10 pm, 10 pm to 50 pm, and/or 50 pm to 100 pm.
  • a combination of hollow fibres of this disclosure which comprises:
  • the porosity of the walls of each hollow fibre of this disclosure is within the range of 1 % to 95 %; preferably, within the range of 50 % to 90 %; and most preferably, within the range of 60 % to 80 %.
  • the porosity of the walls of each hollow fibre of this disclosure is within the range of 1 % to 20 %, 20 % to 40 %, 40 % to 60 %, 60 % to 80 %, or 80 % to 90 %.
  • the porosity of the walls of each hollow fibre of this disclosure is below 1 %.
  • Mercury porosimetry is the gold standard for the measurement of pore volume, porosity and pore size of semi-permeable porous hollow fibres. Based on the capillary law for small pores, porosity may be determined by the total penetrating volume of a non-wetting fluid per unit volume of sample. Pore size may be calculated from the Washburn equation. Modern mercury porosimetry may measure pore diameters ranging between 900 pm to 0.005 pm (35).
  • surface pore diameter may be measured through SEM image analysis (32; 33). Such analytical methods may be applied to the hollow fibres of this disclosure, by someone of skill in the art.
  • the molecular cut-off weight is defined as the lowest molecular weight at which more than 90 % of the solute with a known molecular weight is retained by hollow fibre.
  • MWCO molecular cut-off weight
  • polyethylene glycols, polyethylene oxide) or bovine serum albumin are used as marker molecules to determine the MWCO (36).
  • the change in abundance of these marker molecules in the feed and permeate streams can be measured with refractive index signal intensity separated by size exclusion chromatography with a high-performance liquid chromatography analyser. This method may be used by someone skilled in the art to determine the molecular cut off weight of the hollow fibres of this disclosure.
  • the MWCO of each hollow fibre of this disclosure is within the range of 5 kDa to 100 kDa; preferably, 5 kDa to 7 kDa; more preferably, 7 kDa and 10 kDa; even more preferably, 10 kDa and 20 kDa; and most preferably, 20 kDa and 30 kDa.
  • the MWCO of each hollow fibre of this disclosure is within the range of 5 kDa to 10 kDa, 10 kDa to 15 kDa, 15 kDa to 20 kDa, 20 kDa to 25 kDa, 25 kDa to 30 kDa, 30 kDa to 35 kDa, 35 kDa to 40 kDa, 40 kDa to 45 kDa, 45 kDa to 50 kDa, 50 kDa to 55 kDa, 55 kDa to 60 kDa, 60 kDa to 65 kDa, 65 kDa to 70 kDa, 70 kDa to 75 kDa, 75 kDa to 80 kDa, 80 kDa to 85 kDa, 85 kDa to 90 kDa, 90 kDa to 95 kDa, or 95 kDa to 100 kDa.
  • the trans-wall pure water permeability (PWP) of a hollow fibre of the disclosure is within the range of 10 L/(m 2 h bar) and 1 ,000 L/(m 2 h bar); preferably, 10 L/(m 2 h bar) to 200 L/(m 2 h bar); and most preferably, 0.5 L/(m 2 h bar) to 30 L/(m 2 h bar).
  • the PWP of the hollow fibres of the disclosure is defined as the volume (L) of pure water that passes through the fibre walls per unit area (m 2 ) of the hollow fibre, per unit of time (h), and per unit of trans-wall pressure (bar).
  • a person of skill in the art may be able to determine the PWP of the hollow fibres of this disclosure using a hydraulic filtration system, such as that illustrated in Figure 7.
  • pressure gauges located at the inlet and outlet of cartridge continuously measure the upstream and downstream pressures. Pure water is pumped from a reservoir through the lumen of the hollow fibres in the cartridge, via a positive displacement pump. Alternatively, a gear pump could be used. Pure water may either permeate through the walls of the hollow fibres into the extra capillary space on the shell side or flow through the lumen. The outlet on the shell side of the hollow fibres, transfers liquid into a permeate reservoir. The mass of the reservoirs of feed water and shell side permeate are continuously recorded.
  • the PWP may be determined by measuring the change in mass of water collected on the permeate side of the wall throughout the operation (37; 36).
  • the PWP remains within a suitable range over a typical culture period. Such a period may last at least 3 days.
  • the particle rejection size is defined as being the smallest particle size at which more than 90 % of particles in a standard test slurry of a given uniform particle size is retained by a separation device, such as the hollow fibres of this disclosure, (36).
  • the PRS rejection size of each fibre of this disclosure is within the range of 0.1 to 100 pm; preferably, at least 100 pm; more preferably, at least 50 pm; even more preferably, at least 25 pm; yet more preferably, at least 10 pm; yet even more preferably, at least 5 pm; more preferably still, at least 2.5 pm; even more preferably still, at least 1 pm; yet even more preferably still, at least 0.5 pm; and most preferably, at least 0.1 pm.
  • the PRS of each fibre of this disclosure is within the range of 0.1 pm to 1 pm, 1 pm to 5 pm, 5 pm to 10 pm, 10 pm to 15 pm, 15 pm to 20 pm, 20 pm to 25 pm, 25 pm to 30 pm, 30 pm to 35 pm, 35 pm to 40 pm, 40 pm to 45 pm, 45 pm to 50 pm, 50 pm to 55 pm, 55 pm to 60 pm, 60 pm to 65 pm, 65 pm to 70 pm, 70 pm to 75 pm, 75 pm to 80 pm, 80 pm to 85 pm, 85 pm to 90 pm, 90 pm to 95 pm, or 95 pm to 100 pm
  • a person of skill in the art may be able to determine the PRS of the hollow fibres of this disclosure using a multi-sizer that evaluates the size of particles in slurries comprising a suspension of different-sized polystyrene balls. Once a test slurry is passed through the hollow fibre, the difference in abundance of polystyrene ball sizes in the test slurry and the permeate may then used to calculate the PRS of the hollow fibres of this disclosure (6).
  • the hollow fibres of this disclosure comprise one or more covalent ester, thioester and/or amide crosslinked polypeptides.
  • Prokitein is used for the production of hollow fibres.
  • the hollow fibres comprise Prokitein.
  • the covalent ester, thioester and/or amide crosslinked polypeptides may be multimers, such that they comprise multiple chains of those polypeptides.
  • polypeptides in the hollow fibres of this disclosure are partially covalently-crosslinked, substantially covalently-crosslinked or entirely covalently-crosslinked.
  • the hollow fibres of this disclosure comprise at least 50 % (by weight) covalently-crosslinked polypeptides.
  • the hollow fibres of this disclosure comprise at least 1 %, 5 %, 10 %, 20 %, 30 %, 40 %, 50 %, 60 %, 70 %, 80 %, 90 %, 95 %, 99 % or 99.99 % (by weight) covalently-crosslinked polypeptides.
  • the hollow fibres of this disclosure comprise between 1 % to 5 %, 5 % to 10 %, 10 % to 20 %, 20 % to 30 %, 30 % to 40 %, 40 % to 50 %, 50 % to 60 %, 60 % to 70 %, 70 % to 80 %, 80 % to 90 %, or 90 % to 99.99 % (by weight) covalently-crosslinked polypeptides.
  • the hollow fibres of this disclosure additionally comprise polysaccharides, that are each present in a mass ratio that is within the range of 1 % to 10000 % (weight per weight of polypeptide).
  • the hollow fibres of this disclosure additionally comprise lipids, that are each present in a mass ratio that is within the range of 1 % to 10000 % (weight per weight of polypeptide).
  • the hollow fibres of this disclosure additionally comprise polyols, that are each present in a mass ratio that is within the range of 1 % to 10000 % (weight per weight of polypeptide).
  • the hollow fibres of this disclosure additionally comprise at least one of polysaccharides and lipids, that are each present in a mass ratio that is within the range of 1 % to 10000 % (weight per weight of polypeptide). In some further embodiments, the hollow fibres of this disclosure additionally comprise at least one of polysaccharides, lipids, and polyols, that are each present in a mass ratio that is within the range of 1 % to 10000 % (weight per weight of polypeptide).
  • the polypeptides in the hollow fibres of this disclosure are crosslinked with a combination of intramolecular (within individual polypeptides) and intermolecular (between multiple polypeptides) covalent ester, thioester or amide bonds.
  • polypeptides and polysaccharides in the hollow fibres of this disclosure are crosslinked with a combination of intramolecular and intermolecular to form polypeptidepolypeptide, polypeptides-polysaccharide, polysaccharide-polysaccharide ester, thioester and/or amide covalent bond crosslinks.
  • the covalent crosslinking may be between all or substantially all of polypeptides of the hollow fibres.
  • the covalent crosslinking may be between all or substantially all of the types of polypeptides of the hollow fibres.
  • the covalent crosslinking may be between all, or substantially all, of the types of polypeptides and/or polysaccharides of the hollow fibres.
  • the polypeptides in the hollow fibres of this disclosure are at least 20 % (by weight of polypeptide) covalently-crosslinked; preferably, at least 50 % to 95 %; more preferably, at least 55 % to 90 %; even more preferably, at least 65 % to 85 %; and most preferably, at least 70 % to 80 % crosslinked.
  • the polypeptides in the hollow fibres of this disclosure are at least 20 %, 30 %, 40 %, 50 %, 60 %, 70 %, 80 %, 90 %, 95 %, 99 % or 99.99 % (by weight of polypeptide) covalently-crosslinked.
  • the range of polypeptides in the hollow fibres of this disclosure that are covalently-crosslinked is between 20 % and 30 %, 30 % and 40 %, 40 % and 50 %, 50 % and 60 %, 60 % and 70 %, 70 % and 80 %, 80 % and 90 %, or 90 % and 99.99 % (by weight of polypeptide).
  • Prokitein comprises polypeptides obtained from a single source (e.g. soybeans or faba beans).
  • the disclosure is also based, at least in part, on the realization that Prokitein comprising polypeptides derived from multiple sources (e.g., soybeans and faba beans) may have different properties to Prokitein comprising polypeptides derived from just a single source.
  • sources e.g., soybeans and faba beans
  • a “Prokitein alloy” refers to a Prokitein comprising polypeptides derived from multiple different sources.
  • polypeptides of Prokitein, Prokitein alloy, and/or methods of the disclosure are obtained or derived from plants, animals, bacteria, algae, archaea, and/or fungi.
  • a Prokitein alloy may comprise polypeptides derived from two, three, four, five, six or more than six sources.
  • a Prokitein alloy comprises polypeptides derived from two sources; three sources; four sources; five sources; six sources; or more than six sources.
  • Prokitein comprises a Prokitein alloy.
  • Prokitein may additionally comprise polysaccharides, lipids, polyols, and/or any combination thereof.
  • Prokitein comprises polysaccharides.
  • Prokitein comprises lipids. In some embodiments, Prokitein comprises polyols.
  • Prokitein comprises polysaccharides and lipids.
  • Prokitein comprises lipids and polyols.
  • Prokitein comprises polysaccharides and polyols.
  • Prokitein comprises polysaccharides, lipids and polyols.
  • the polypeptides in the hollow fibres may be crosslinked through esterification, thioesterification and/or amidation reactions with polycarboxylic acids.
  • the polypeptides in the hollow fibres may be crosslinked through esterification, thioesterification and/or amidation reactions with polycarboxylic acid salts that include, but are not limited to, sodium oxalate, potassium oxalate, sodium malate, potassium malate, sodium succinate, potassium succinate, sodium adipate, potassium adipate, sodium tartrate, potassium tartrate, potassium citrate, sodium citrate, sodium malonate, and/or potassium malonate; and preferably, sodium citrate and/or sodium malate.
  • polycarboxylic acid salts include, but are not limited to, sodium oxalate, potassium oxalate, sodium malate, potassium malate, sodium succinate, potassium succinate, sodium adipate, potassium adipate, sodium tartrate, potassium tartrate, potassium citrate, sodium citrate, sodium malonate, and/or potassium malonate; and preferably, sodium citrate and/or sodium malate.
  • Polycarboxylic acids with more than one carboxyl groups may be used to form inter-polypeptide (i.e. , in between two polypeptide chains) and/or intra-polypeptide (i.e. , in between two locations along the one polypeptide chain) covalent ester, thioester or amide crosslinks (20).
  • polycarboxylic acids with more than one carboxyl groups are used to form inter-polypeptide and/or intra-polypeptide covalent ester, thioester or amide crosslinks.
  • Polycarboxylic acids with more than one carboxyl groups may be used to form inter-polypeptide and/or intra-polypeptide covalent ester crosslinks in the presence of a secondary catalyst, such as sodium hypophosphite.
  • the two carboxyl groups may initially form a cyclic anhydride at high temperatures (38; 39).
  • the cyclic anhydride may then react with one of the amine (-NH 2 ), hydroxide (-OH), or thiol (-SH) functional groups present on the polypeptide chain to potentially form a polypeptide ester, thioester or amide.
  • a secondary addition reaction between the polycarboxyl derivative and sodium hypophosphite may then form an intermediate which may react with a second polypeptide ester, thioester or amide thereby potentially creating a crosslink.
  • Other reaction pathways with bi-functional polycarboxylic acids may be possible, and catalysts may also be used (23; 40).
  • polycarboxylic acids with more than one carboxyl groups are used to form inter-polypeptide and/or intra-polypeptide covalent ester crosslinks in the presence of a secondary catalyst, such as sodium hypophosphite.
  • Polycarboxylic acids with more than two carboxyl groups may be used to form inter-polypeptide and/or intra-polypeptide covalent ester crosslinks.
  • initially two of the carboxyl groups on the polycarboxylic acid may be dehydrated to form a cyclic anhydride.
  • the cyclic anhydride may then react with one of the amine (-NH 2 ), hydroxide (-OH) or thiol (-SH) functional groups present on the polypeptide chain to potentially form a polypeptide ester, thioester or amide with two or more carboxyl groups.
  • polypeptide ester Two of the carboxyl groups on the polypeptide ester, thioester or amide may then be dehydrated again to potentially form a cyclic anhydride.
  • polypeptide ester with a cyclic anhydride may then react with a functional group on a second polypeptide chain to potentially form a polycarboxylic acid derived ester, thioester or amide crosslinked polypeptide (20; 41).
  • polycarboxylic acid salts with more than two carboxyl groups are used to form inter-polypeptide and/or intra-polypeptide covalent ester, thioester or amide crosslinks.
  • polycarboxylic acid salts with more than two carboxyl groups are used to form inter-polypeptide and/or intra-polypeptide covalent ester, thioester or amide crosslinks in the presence of a catalyst.
  • Polycarboxylic acids with more than two carboxyl groups may be used to form inter-polypeptide and/or intra-polypeptide covalent ester crosslinks in the presence of a secondary catalyst, such as sodium hypophosphite.
  • a secondary catalyst such as sodium hypophosphite.
  • the two carboxyl groups may initially be dehydrated to potentially form a cyclic anhydride. Subsequent acylation between the polycarboxylic acid derived anhydride and sodium hypophosphite may result in the formation of an intermediate.
  • Nucleophilic substitution of the anhydride and sodium hypophosphite intermediate with one of the amine (-NH 2 ) hydroxide (-OH), and thiol (-SH) functional groups present on the polypeptide chain may then produce a polypeptide ester, thioester or amide with two or more carboxyl groups. Two of the carboxyl groups on the polypeptide polycarboxylic acid derived ester, thioester or amide may then be dehydrated again to potentially form a polypeptide ester, thioester or amide cyclic anhydride intermediate.
  • catalysts used to catalyse the formation of polycarboxylic acid derived inter-polypeptide and/or intra-polypeptide covalent ester, thioester or amide crosslinks include, but are not limited to, sodium sulphite, sodium bisulphite, sodium metabisulphite, and/or sodium hypophosphite.
  • a person of skill in the art may be able determine the chemical composition and structure of the polypeptides in the hollow fibres of this disclosure as well as the polypeptides from which they are derived. Methods which may be used include mass spectrometry (MS), SDS-PAGE, Fourier Transform Infrared (FTIR) Spectroscopy, circular dichroism, Wide-Angle X-ray Scattering (WAXS) and Raman Spectroscopy.
  • MS mass spectrometry
  • SDS-PAGE SDS-PAGE
  • FTIR Fourier Transform Infrared
  • WAXS Wide-Angle X-ray Scattering
  • Raman Spectroscopy Raman Spectroscopy.
  • a person of skill in the art may be able determine the chemical composition and structure of the polypeptides in Prokitein as well as the polypeptides from which they are derived, using the same methods applicable to the hollow fibres of this disclosure. Methods which may be used include mass spectrometry (MS), SDS-PAGE, Fourier Transform Infrared (FTIR) Spectroscopy, circular dichroism, Wide-Angle X-ray Scattering (WAXS) and Raman Spectroscopy.
  • MS mass spectrometry
  • SDS-PAGE SDS-PAGE
  • FTIR Fourier Transform Infrared
  • WAXS Wide-Angle X-ray Scattering
  • Raman Spectroscopy Raman Spectroscopy.
  • MS analysis is generally performed on protein samples that are either fully intact (top-down) or have been initially digested into fragments (bottom-up).
  • samples are initially converted into an ionised gas, through methods such as electrospray ionization or matrix-assisted laser desorption/ionization (MADLI).
  • MADLI matrix-assisted laser desorption/ionization
  • the mass(es) of the ionised sample or sample fragments may then be determined with a time of flight (TOF) detector.
  • TOF time of flight
  • Peptide sequences may then be determined by comparison of recorded sample fragments and predicted peptide sequence masses.
  • methods such as SDS-PAGE, high-performance liquid chromatography (HPLC), or gas chromatography (GC) may be used to separate protein mixtures prior to analysis (42).
  • SDS-PAGE Sodium dodecyl sulphate-polyacrylamide gel electrophoresis
  • SDS acts to denature and unfold most protein structures to eliminate differences in the secondary and tertiary structure of polypeptides, while minimising the effect of their surface charge differences. Consequently, since the 1970s, SDS-PAGE has been one of the most widely used techniques for protein separation and characterisation.
  • the mass of unknown bands may be estimated. Cross-referencing these estimated masses with MS or western blot data from the literature may then be used for the identification of bands isolated in a sample (43).
  • the degree of crosslinking in the crosslinked polypeptide sample may be estimated by the multiplicity of mass of the known bands associated with the bands in the known polypeptide sample.
  • SDS-PAGE has been used in the identification of polypeptides present in soy protein, which may be separated into four major groups designated as 2S, 7S, 11S, and 15S.
  • 7S globulin beta-conglycinin
  • 11 S globulin globulin
  • alpha alpha
  • alpha alpha
  • beta beta
  • Glycinin ( ⁇ 350 kDa) is a hexamer composed of five major subunits with two subunits being basic ( ⁇ 19.6 kDa) or acidic ( ⁇ 34.8 kDa) (44; 45).
  • the degree of cross linking in a soy polypeptide derived crosslinked sample may be estimated. Such methods may be used to analyse polypeptides of other origins.
  • the mass of bands identified may be estimated. These bands can then be identified through comparison with known masses from the literature in order to determine the components in the starting organic material. Bands with masses which do not correlate with those in literature may be identified with mass spectrometry.
  • the mass of bands identified may be estimated. These bands can then be identified through comparison with known masses from the literature in order to determine the components in the starting organic material. Bands with masses which do not correlate with those in literature may be identified with mass spectrometry.
  • FTIR Fourier Transform infrared
  • Secondary structure is commonly determined from these bands via one of two methods: (1) fitting component bands to the amide I band; or (2) decomposing the amide I band into basis spectra determined from calibration proteins (46).
  • FTIR has been used to evaluate the change in the secondary structure of silk proteins upon exposure to organic solvents.
  • the components of the secondary structure of silk proteins were quantified from the peak areas of their assigned wavelengths: beta-sheets (1616-1637 cm -1 and 1697-1703 cm -1 ), random coils (1638-1655 cm’ 1 ), alpha helices (1656-1662 cm’ 1 ) and beta turns (1663- 1696 cm’ 1 ), (27; 28).
  • FTIR may be used to evaluate changes in the secondary structure of the polypeptides of the hollow fibres of this disclosure.
  • Circular dichroism is an inexpensive and rapid method for the evaluation of protein secondary structures that is based on the differential adsorption of left- and right-handed polarized light.
  • Various structural elements in polypeptide backbones interact with light such that while in specific conformations they yield a characteristic CD spectrum.
  • alphahelical proteins have negative bands at 222 nm and 208 nm and a positive band at 193 nm.
  • Proteins with beta-helices have negative bands at 218 nm and positive bands at 195 nm, while disordered polypeptides have very low ellipticity above 210 nm and negative bands near 195 nm.
  • CD may be used to evaluate changes in the secondary structure of the polypeptides of the hollow fibres of this disclosure.
  • X-ray diffraction is a well-established technique used in the characterisation of the crystalline structure of materials, including polypeptides (48). Wide-angle X-ray scattering can be used in the determination of protein secondary structure, through the association of peaks at 9° and 20° being associated with changes in alpha-helices and beta-sheets respectively (49). Hence, XRD may be used to evaluate changes in the secondary structure of the polypeptides of the hollow fibres of this disclosure.
  • Raman spectroscopy may be used for the analysis of polypeptide structures through the use of several vibrational modes. By considering the vibrational bands associated with amides A, B, and I to VII, different polypeptide secondary structure conformations may be identified. The relative abundance of alpha-helix and beta-sheets in polypeptide samples may be determined from the positions of amides I and III. Specifically, the average wavelengths for alpha-helices are: 1662-1655 and 1272-1264 cm -1 , while for beta-sheet structures are 1674-1672 and 1242- 1227 cm -1 , respectively, for amide I and amide III modes (50). Hence, Raman spectroscopy may be used to evaluate changes in the secondary structure of the polypeptides of the hollow fibres of this disclosure.
  • FTIR, CD, XRD and Raman spectroscopy may also be used to evaluate changes in the secondary structure of the polypeptides of Prokiteins.
  • DSC Differential Scanning Calorimetry
  • AH C annealing peak
  • AH f melting peak
  • T m melting point
  • Annealing may be used to induce crystal formation in Prokitein and the hollow fibres of this disclosure. During annealing, samples are bought up to a temperature between the glass transition temperature and the melting point, which may enable molecular chains to rearrange into more stable forms with reduced internal stresses. Upon subsequent cooling, the molecular chains may be frozen in place.
  • Samples which have been annealed, when analysed with DSC, may exhibit an increase in melting temperature, an increase in crystallisation temperature, a sharper and more pronounced melting peak, and/or a reduction in the number of melting peaks.
  • re-annealing a sample which has already been annealed may result in only limited changes to the thermal properties of the sample, as the crystal structure has already been stabilised to a degree (i.e. , with reduced internal stresses). Therefore, one of ordinary skill in the art may be able to use DSC to assess if a polymer, such as Prokitein or a hollow fibre of this disclosure, has been annealed.
  • XRD can also be used by one of ordinary skill in the art to assess the degree of crystallinity of Prokitein and/or the hollow fibres of this disclosure.
  • crystalline regions typically produce sharp peaks, while amorphous regions usually contribute to a broad, diffuse background.
  • the degree of crystallinity may be assessed by integrating the relative magnitude of the peaks in an XRD spectra.
  • a semi-crystalline polymer, such as Prokitein may yield an XRD spectra that comprises the peaks and broad, diffuse background associated with crystalline and amorphous regions, respectively.
  • the internal stresses of Prokitein and hollow fibres of this disclosure may be assessed by analysis of XRD spectra using methods comprising Bragg’s Law, Analysis of Peak shifts, Sin 2 ip method associated with tilt angles, and/or whole powder pattern fitting. Furthermore, the uniform distribution of crystal grain size and shapes may be determined through the analysis of peaks within a XRD spectra by one of ordinary skill in the art.
  • the XRD spectra of Prokitein comprises sharp peaks and a broad diffuse background.
  • Prokitein is almost entirely crystalline.
  • Prokitein is almost entirely amorphous.
  • the crystallinity of Prokitein is higher than 0 %.
  • the crystallinity of Prokitein is higher than 5 %. In some embodiments, the crystallinity of Prokitein is higher than 10 %.
  • the crystallinity of Prokitein is higher than 20 %.
  • the crystallinity of Prokitein is higher than 30 %.
  • the crystallinity of Prokitein is higher than 40 %.
  • the crystallinity of Prokitein is higher than 50 %.
  • the crystallinity of Prokitein is higher than 60 %.
  • the crystallinity of Prokitein is higher than 70 %.
  • the crystallinity of Prokitein is higher than 80 %.
  • the crystallinity of Prokitein is higher than 90 %.
  • the crystallinity of Prokitein is higher than 95 %.
  • the crystallinity of Prokitein is within the range of 0.01 % to 10 %, 10 % to 20 %, 20 % to 30 %, 30 % to 40 %, 40 % to 50 %, 50 % to 60 %, 60 % to 70 %, 70 % to
  • the ratio of crystalline to amorphous regions within Prokitein may be altered such as to tailor its material properties. Crystalline materials typically are stiff, hard, and brittle, while amorphous are flexible. The ratio of crystalline to amorphous regions within Prokitein may be tailored by the temperature and duration by which it is annealed.
  • the ratio of crystalline to amorphous regions within Prokitein is altered and/or tailored by protein annealing. FUNCTIONAL CHARACTERISTICS OF THE COVALENTLY-CROSSLINKED, POROUS, SEMI-PERMEABLE HOLLOW FIBRES
  • the hollow fibres of the disclosure remain mechanically stable whilst submerged in an aqueous solution for at least a day, and preferably for at least 3 days.
  • Step (d) and Step (e.i) promotes the formation of betasheet structures within the polypeptides of the hollow fibres of this disclosure.
  • the formation of beta-sheets in the secondary structure of the polypeptides confers a degree of water stability of the hollow fibres produced in this disclosure.
  • Step (e.i) may promote the formation of beta-sheets within the secondary protein structure of the crosslinked polypeptides of the hollow fibres of this disclosure, thereby potentially granting them a degree of water stability.
  • Step (e.ii) anneals the covalently crosslinked hollow fibres, thereby potentially relaxing internal stresses in the crosslinked material, and may allow the beta-sheet configuration to remain intact upon submersion in aqueous solvents, which may prevent the hydrolytic hydrolysis of the covalent ester crosslinks.
  • the hollow fibres of this disclosure may maintain good mechanical properties after being continuously submerged in an aqueous solution.
  • the hollow fibres of this disclosure maintain good mechanical properties after being continuously submerged in an aqueous solution.
  • at least one of the Young’s modulus, ultimate tensile strength, and/or ultimate tensile strain of the hollow fibres of this disclosure do not decrease by more than 60 % after being submerged in an aqueous solution, such as PBS, at a temperature between 18 °C and 38 °C and with a pH between 7.0 and 8.0 for a period of 3 days; preferably after a period of 5 days; more preferably after a period of 10 days; even more preferably after a period of 15 days; and most preferably after a period of 30 days.
  • the hollow fibres of this disclosure maintain good structural properties after being continuously submerged in an aqueous solution.
  • the wall thickness, pore diameter, and pore void fraction, as measured with SEM and/or mercury porosimetry, of the hollow fibres produced in this disclosure do not change by more than 30 % after being submerged in an aqueous solution, such as PBS, at a temperature between 18 °C and 38 °C and with a pH between 7.0 and 8.0 for a period of 3 days; preferably after a period of 5 days; more preferably after a period of 10 days; even more preferably after a period of 15 days; and most preferably after a period of 30 days.
  • the resulting displacement may be recorded as a function of the force applied until sample failure with the use of universal uni-axial tensile testing.
  • the mechanical characteristics of a material may be calculated from the measured relationship between the tensile force applied to the sample, and the resulting displacement. Knowing the cross-sectional area of the material tested, as measured with SEM, characteristics which may be calculated include the yield point, ultimate tensile strength, ultimate tensile strain, and the Young’s modulus.
  • the ultimate tensile strength is the maximum force applied to the material before failure.
  • the ultimate tensile strain is the material elongation at the point of failure.
  • the Young’s modulus is the ratio of the change in stress over the change in strain before the yield point.
  • the yield point is the point at which the relationship between the stress and strain is no longer directly proportional.
  • the yield point, ultimate tensile strength, ultimate tensile strain, and the Young’s modulus of Prokiteins may also be evaluated by universal uni-axial tensile testing.
  • the hollow fibres of this disclosure maintain good aqueous fluid transport characteristics after being submerged in aqueous solution.
  • the PWP of the hollow fibres produced in this disclosure does not decrease by more than 60 %, nor increase by more than 20 %, after being submerged in an aqueous solution, such as phosphate buffer solution (PBS), at a temperature between 18 °C and 38 °C and with a pH between 7.0 and 8.0 for a period of 3 days; preferably for a period of 5 days; more preferably for a period of 10 days; even preferably after a period of 15 days; and most preferably after a period of 30 days.
  • PBS phosphate buffer solution
  • the hollow fibres of this disclosure maintain good mass transport characteristics after being submerged in aqueous solution.
  • both the molecular cut-off weight and particle retention size of the hollow fibres produced in this disclosure do not decrease by more than 60 %, nor increase by more than 20 % after being submerged in an aqueous solution, such as phosphate buffer solution (PBS), at a temperature between 18 °C and 38 °C and with a pH between 7.0 and 8.0 for a period of 3 days; preferably for a period of 5 days; more preferably for a period of 10 days; even preferably after a period of 15 days; and most preferably after a period of 30 days.
  • PBS phosphate buffer solution
  • the treatment of the hollow fibres to alter their mechanical properties may be carried out either before and/or after the culture of cells on the hollow fibres.
  • the treatment of the hollow fibres to alter their mechanical properties are carried out before the culture of cells on the hollow fibres.
  • the treatment of the hollow fibres to alter their mechanical properties are carried out after the culture of cells on the hollow fibres.
  • the treatment of the hollow fibres to alter their mechanical properties is not carried out.
  • the hollow fibres of this disclosure are stable whilst submerged in an aqueous solution, such as phosphate buffer solution (PBS), at a temperature between 18 °C and 38 °C and with a pH between 7.0 and 8.0 for a period of 3 days, such that the hollow fibres maintain good mechanical properties, structural characteristics, aqueous fluid transport properties, and mass transport properties for a period of at least 3 days; preferably for a period of 5 days; more preferably for a period of 10 days; even preferably after a period of 15 days; and most preferably after a period of 30 days.
  • PBS phosphate buffer solution
  • Prokitein remains mechanically stable whilst submerged in an aqueous solution for at least a day, preferably for at least 3 days; more preferably, for at least 5 days; even more preferably, for at least 10 days; yet even more preferably, for at least 15 days; and most preferably for at least 30 days.
  • Prokitein remains mechanically stable whilst submerged in an aqueous solution for at least 1 month, preferably for at least 3 months; and most preferably for at least 6 months.
  • Prokitein maintains good mechanical properties after being continuously submerged in an aqueous solution.
  • at least one of the Young’s modulus, ultimate tensile strength, and/or ultimate tensile strain of Prokitein, as measured directly after removal from aqueous solutions does not decrease by more than 60 % after being submerged in an aqueous solution, such as PBS, at a temperature between 18 °C and 38 °C and with a pH between 7.0 and 8.0, for a period of 3 days; preferably after a period of 5 days; more preferably after a period of 10 days; even more preferably after a period of 15 days; and most preferably after a period of 30 days.
  • the hollow fibres of this disclosure are edible.
  • Prokitein of this disclosure is edible.
  • the hollow fibres of this disclosure are suitable for human and/or animal ingestion.
  • Prokitein of this disclosure is suitable for human and/or animal ingestion.
  • the hollow fibres of this disclosure are GRAS.
  • the hollow fibres of this disclosure are produced solely from components which are GRAS.
  • the Prokitein of this disclosure is produced solely from components which are GRAS. In some embodiments, the hollow fibres of this disclosure are produced in part from components which are GRAS.
  • the Prokitein of this disclosure is produced in part from components which are GRAS.
  • the hollow fibres of this disclosure are palatable.
  • the Prokitein of this disclosure is palatable.
  • the hollow fibres of this disclosure constitute a food product.
  • the Prokitein of this disclosure constitute a food product.
  • the hollow fibres of this disclosure are a food product.
  • the Prokitein of this disclosure is a food product.
  • the hollow fibres of this disclosure as an ingredient in a food product.
  • the Prokitein of this disclosure as an ingredient in a food product.
  • the hollow fibres of this disclosure are biodegradable.
  • the Prokitein of this disclosure is biodegradable.
  • the term “edible” is defined as a material which is fit for human and/or animal consumption.
  • the term “edible” refers to a material which is fit for human and/or animal consumption and is capable of being designated as food. While it cannot be ensured that a particular item of food will never pose a risk to a consumer, various risk management frameworks exist to control the potential risks. This is embodied as pre-market authorisation decisions by food safety authorities.
  • the European Food Safety Authority EFSA
  • Novel Food Regulation EU 2015/2283.
  • a particular food is considered to be “novel” if it falls within a number of defined categories, and was not significantly consumed within the EU prior to 15 May 1997.
  • the defined categories include: food with a new or intentionally modified molecular structure, where that structure was not used as, or in, food within the EU before 15 May 1997.
  • the term “edible” refers to material that qualifies as food according to the European Food Safety Authority.
  • the term “edible” refers to material that qualifies as food according to the European Food Safety Authority as of 1 st January 2023.
  • the United States Food and Drug Administration designates foods or food additives as being ‘Generally Recognised as Safe’ (GRAS) for consumption based on consensus among qualified experts. There are two ways in which this designation is be given, either through: (i) long-standing use, in which the food has been widely consumed since before 1958 and there are no reports of harm; or (ii) a series of scientific safety studies and subsequent review of the generated data by the FDA.
  • GRAS Generally Recognised as Safe
  • GRAS are used interchangeably and mean a material which is fit for human or animal consumption.
  • GRAS means a material that qualifies as GRAS pursuant to the United States Food and Drug Administration.
  • GRAS means a material that qualifies as GRAS pursuant to the United States Food and Drug Administration as 1 st January 2023.
  • the term “edible” means a material that is GRAS.
  • “edible” refers to a material that both qualifies as food according to the European Food Safety Authority and is GRAS.
  • the EFSA has published guidance for the assessment of food safety under the Novel Food Regulation (EU) 2015/2283. These regulations mandate scientific data on the kinetics and toxicity of the proposed novel food, and includes listings of the identity of the novel food, a detailed description of the production process, compositional information, stability data, absorption, distribution, metabolism and excretion (ADME) data, as well as nutritional, and toxicological information. Toxicological data should include detailing of the anticipated use, kinetic data, toxicological data of the constitutive materials, human studies data and available relevant information.
  • Petrochemical polymer and ceramic hollow fibres are not and have not been widely consumed in the EU since before 1997, nor have they been consumed in the US since before 1958. They lack special designation by the EFSA as novel foods or by the FDA as GRAS. These materials may not be safely ingested.
  • the hollow fibres of this disclosure are non-cytotoxic.
  • Edible extruded or spun, fibres of this disclosure may comprise covalent ester, thioester and/or amide crosslinked polypeptides, and may be used as a substrate for cell growth in cell culture or within a bioreactor. To ensure that the edible hollow fibres produced in this disclosure are suitable for cell culture, they are in certain embodiments non-cytotoxic.
  • the International Organization for Standardization (ISO) standard ISO 10993-1 :2018 has been ratified for the selection of biological materials by specifying testing procedures to demonstrate carcinogenicity, genotoxicity, hemocompatibility, cytotoxicity, implantation, sensitization, irritation, acute, sub-acute, sub-chronic and chronic toxicity. Cytotoxic effects may be determined by either qualitative or quantitative methods. However, quantitative methods are preferable and qualitative methods should be used for screening purposes only. Materials produced in this disclosure may, therefore, in some embodiments be classified as non-cytotoxic as quantified by one of the methods outlined in Annexes A-D in ISO 10993-5 (Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity).
  • the outer or inner surfaces of the hollow fibres of this disclosure are capable of acting as a substrate for the adherence and growth of cells.
  • the hollow fibres of this disclosure are able to sustain a cell culture for at least one day; preferably, for at least 3 days; more preferably, for at least 5 days; more even preferably, for at least 10 days; even more preferably, for at least 15 days; and most preferably, for at least 30 days.
  • the hollow fibres of this disclosure are able to sustain a cell culture for a duration that is within the range of 1 second to 1 minute, 1 minute to 1 hour, 1 hour to 6 hours, 6 hours to 1 day, 1 day to 2 days, 2 days to 3 days, 3 days to 4 days, 4 days to 5 days, 5 days to 6 days, 6 days to 7 days, 7 days to 8 days, 8 days to 9 days, 9 days to 10 days, 10 days to 11 days, 11 days to 12 days, 12 days to 13 days, 13 days to 14 days, 14 days to 15 days, 15 days to 16 days, 16 days to 17 days, 17 days to 18 days, 18 days to 19 days, 19 days to 20 days, 20 days to 21 days, 21 days to 22 days, 22 days to 23 days, 23 days to 24 days, 24 days to 25 days, 25 days to 26 days, 26 days to l days, 27 days to 28 days, 28 days to 29 days, 29 days to 30 days, or more than 30 days.
  • Substrates may be appropriate for cell growth as characterised in terms of their cytotoxicity.
  • ISO 10993 ratifies standardised methods for determining the cytotoxicity of materials and the means by which they should be tested according to their structure. Materials may only be considered non-cytotoxic if they satisfy the test protocols as dictated by ISO 10993 (52).
  • Someone of skill in the art may evaluate the cytotoxicity of the extruded or spun, fibres of this disclosure comprising covalent ester, thioester and/or amide crosslinked polypeptides, using the methods outlined in ISO 10993.
  • the hollow fibres of this disclosure are non-cytotoxic, as determined by methods described in ISO 10993.
  • Cell proliferation is defined as the increase in the number of cells as a result of cellular growth and division.
  • the number of cells in a given sample may be directly and/or indirectly quantified by someone of skill in the art using a number of on-line and off-line methods.
  • Direct cell counts are most commonly taken, performed under a microscope either manually or with a haemocytometer. Other direct methods may include flow cytometry, and spectrophotometry.
  • indirect measures may be employed, such as recording the trends in concentrations of metabolites (e.g., biomass, glucose, ethanol, lactic acid, acetic acid, succinic acid, and citric acid) and dissolved gases (e.g., oxygen, carbon dioxide, and ammonia).
  • metabolites e.g., biomass, glucose, ethanol, lactic acid, acetic acid, succinic acid, and citric acid
  • dissolved gases e.g., oxygen, carbon dioxide, and ammonia
  • Cell number may also be indirectly measured with metabolic assays such as Alamar blue or CellTiter Gio. Furthermore, samples may be frozen to get a snapshot into the culture performance at a given time point. These samples may then be imaged with techniques such as confocal microscopy, SEM, or histology. Such methods may be applied to assess the proliferation of cells grown on the hollow fibres of this disclosure.
  • metabolic assays such as Alamar blue or CellTiter Gio.
  • samples may be frozen to get a snapshot into the culture performance at a given time point. These samples may then be imaged with techniques such as confocal microscopy, SEM, or histology. Such methods may be applied to assess the proliferation of cells grown on the hollow fibres of this disclosure.
  • cell number may also be indirectly measured with metabolic assays utilising PrestoBlue, PrestoBlue High-Sensitivity and/or solutions comprising of resazurin salt.
  • hollow fibres of this disclosure are suitable for use as components in a bioreactor that is intended for use in cell culture.
  • the hollow fibres of this disclosure are suitable for use as components in a multimodal, mechanically dynamic bioreactor that is intended for use in cell culture.
  • the hollow fibres of this disclosure are suitable for use as components in hydrostatic cell culture, such as in well plates or flasks.
  • multimodal herein refers to the provision of chemical, electrical and/or mechanical stimuli to the cultured cells.
  • mechanically dynamic herein refers to the ability of the bioreactor to apply controlled mechanical forces to the cultured cells.
  • Such a conceived multimodal, mechanically dynamic bioreactor may be used to under dynamic fluid conditions and/or mechanically dynamic conditions, in which the bioreactor platform and/or the cell substrate is either stretched or compressed.
  • the hollow fibres of this disclosure are suitable for being consumed alone or in combination with a product intended for human and/or animal ingestion.
  • the hollow fibres of this disclosure are non-cytotoxic and can be used to sustain cell growth for at least three days. In some other embodiments, the hollow fibres of this disclosure are suitable for use in the production of one or more cultivated meat products intended for human and/or animal consumption.
  • the fibres of this disclosure are suitable for use as components within a bioreactor.
  • one or more of the hollow fibres of this disclosure are used in a bioreactor.
  • the disclosure provides a bioreactor comprising one or more of the hollow fibres of this disclosure.
  • a bioreactor comprising one or more of the hollow fibres of this disclosure is suitable for facilitating cell cultivation.
  • a bioreactor comprising one or more of the hollow fibres of this disclosure is suitable for use in the production of one or more cultivated meat products intended for human and/or animal consumption.
  • the hollow fibres of this disclosure are suitable for use as components in a bioreactor cartridge, wherein each cartridge comprises one or a plurality of the hollow fibres of this disclosure.
  • the cartridges of this disclosure may comprise one or a plurality of the hollow fibres of this disclosure aligned in parallel along the long axis of the hollow fibre, and bounded by an outer wall, with an inlet port and an outlet port.
  • a schematic diagram of a quarter cut out of a section of such a cartridge may be seen in Figure 5.
  • typical components of a cartridge are labelled, including the inlet to the cartridge (5001), the cartridge cap media dissipation cut (5002), cap of the cartridge (5003), the hollow fibre potting layer (5004), a bundle of a hollow fibres (5005) and the outer shell of the cartridge (5006).
  • an intra-capillary (IC) space may be present within the lumen of the hollow fibres of this disclosure, and an extra-capillary (EC) space may be present surrounding the hollow fibres and bounded by the outer shell of the cartridge.
  • IC intra-capillary
  • EC extra-capillary
  • an intra-capillary (IC) space is present within the lumen of the hollow fibres of this disclosure, and an extra-capillary (EC) space is present surrounding the hollow fibres and bounded by the outer shell of the cartridge.
  • IC intra-capillary
  • EC extra-capillary
  • the Krogh cylinder model is often used to model the distance between capillaries in vivo before oxygen concentrations limit cell growth (53).
  • the Krogh cylinder model suggests that hollow fibres should bundled such that there is a distance of no more than 100 pm (54).
  • the shortest distance between any two adjacent fibres of this disclosure in any particular cartridge bundle is within the range of 0 pm to 150 pm; preferably, within the range of 20 pm to 200 pm; more preferably, within the range of 50 pm and 130 pm; even more preferably; within the range of 80 pm and 120 pm; and most preferably, within the range of 50 pm and 130 pm;
  • the shortest distance between any two adjacent fibres of this disclosure, in any particular cartridge bundle is within the range of 0 pm to 10 pm; 10 pm to 20 pm; 20 pm to 30 pm; 30 pm to 40 pm; 40 pm to 50 pm; 50 pm to 60 pm; 60 pm to 70 pm; 70 pm to 80 pm; 80 pm to 90 pm; 90 pm to 100 pm; 110 pm to 120 pm; 120 pm to 130 pm; 130 pm to 140 pm; 140 pm to 150 pm; 150 pm to 160 pm; 160 pm to 170 pm; 170 pm to 180 pm; 180 pm to 190 pm; and/or 190 pm to 200 pm;
  • the shortest distance between any two adjacent fibres of this disclosure, in any particular cartridge bundle is within the range of 200 pm to 250 pm; 250 pm to 300 pm; 300 pm to 350 pm; 350 pm to 400 pm; 400 pm to 450 pm; 450 pm to 500 pm; 500 pm to 550 pm; 550 pm to 600 pm; 600 pm to 650 pm; 650 pm to 700 pm; 700 pm to 750 pm; 750 pm to 800 pm; 800 pm to 850 pm; 850 pm to 900 pm; 900 pm to 950 pm; and/or 950 pm to 1000 pm.
  • the cartridges of this disclosure comprise a plurality of the hollow fibres of this disclosure arranged into one or more bundles that have densities that are within the range of 1 hollow fibres/cm 2 to 1000 hollow fibres/cm 2 ; preferably, 60 hollow fibres/cm 2 to 500 hollow fibres/cm 2 ; and most preferably, 80 hollow fibres/cm 2 to 300 hollow fibres/cm 2 .
  • the cartridges of this disclosure comprise a plurality of the hollow fibres of this disclosure arranged into one or more bundles that have densities that are within the range of 25 hollow fibres/cm 2 to 150 hollow fibres/cm 2 ; preferably, 50 hollow fibres/cm 2 to 125 hollow fibres/cm 2 ; more preferably, 75 hollow fibres/cm 2 to 105 hollow fibres/cm 2 ; and most preferably, 70 hollow fibres/cm 2 to 95 hollow fibres/cm 2 ;
  • the cartridges of this disclosure comprise a plurality of the hollow fibres of this disclosure, that are arranged into one or more bundles that have densities that are within the range of 40 hollow fibres/cm 2 to 60 hollow fibres/cm 2 , 60 hollow fibres/cm 2 to 80 hollow fibres/cm 2 , 80 hollow fibres/cm 2 to 100 hollow fibres/cm 2 , 100 hollow fibres/cm 2 to 120 hollow fibres/cm 2 , 120 hollow fibres/cm 2 to 180 hollow fibres/cm 2 , 180 hollow fibres/cm 2 to 220 hollow fibres/cm 2 , 220 hollow fibres/cm 2 to 250 hollow fibres/cm 2 , 250 hollow fibres/cm 2 to 300 hollow fibres/cm 2 , 300 hollow fibres/cm 2 to 350 hollow fibres/cm 2 , 350 hollow fibres/cm 2 to 400 hollow fibres/cm 2 , 400 hollow fibres/cm 2 to 450 hollow fibres/c
  • the hollow fibres may be secured within a non-cytotoxic matrix through a process referred to as potting.
  • Potting agents which may be used to ensure bundle integrity include, but are not limited to, epoxy, plaster, gypsum plaster, silicon rubber, and polytetrafluoroethylene.
  • potting agents which are used to ensure bundle integrity include crosslinked polypeptides and/or polysaccharides.
  • potting agents which are used to ensure bundle integrity include crosslinked polypeptides and/or polysaccharides that include, covalent ester, thioester and/or amide crosslinks.
  • the cartridges of this disclosure comprise hollow fibres of this disclosure which have been potted with a potting agent.
  • Potting is the process in which the ends of hollow fibres may be encapsulated in a matrix to create a tight fluid seal between the hollow fibres and the outer wall of the bounding capsule. This process is typically required to prevent fluid from bypassing the hollow fibres and flowing into the extra-capillary space, but instead forces the fluid flow into the intra-capillary space.
  • Potting agents are the encapsulating matrix used in the potting process.
  • cartridges of hollow fibres are assembled by reversibly encasing one or multiple bundles of the hollow fibres of this disclosure within an outer shell, and adding suitable end-caps.
  • the cartridges are able to be easily disassembled.
  • individual, multiple and/or bundles of the hollow fibres of this disclosure are removable from a cartridge assembly.
  • individual, multiple or bundles of the hollow fibres of this disclosure are not removable from a cartridge assembly.
  • individual, multiple or bundles of the hollow fibres of this disclosure are removable from a cartridge assembly with cells attached.
  • a cultivated meat product which contains the hollow fibres of this disclosure, is removable from the cartridge assembly.
  • a cultivated meat product which contains the hollow fibres of this disclosure can be further treated to improve, preserve and/or alter the palatability, mouthfeel, taste and/or texture of the cultivated meat product before being removed from the cartridge assembly.
  • the inner diameter of the outer shell of each bioreactor cartridge of this disclosure may be within the range of 1 cm and 5 m.
  • the inner diameter of the outer shell of each bioreactor cartridge of this disclosure is within the range of 1 cm to 50 cm; preferably, the inner diameter is 10 cm; more preferably, the inner diameter is 3 cm; and most preferably, the inner diameter is 7.5 cm.
  • the inner diameter of the outer shell of each bioreactor cartridge of this disclosure is 1 cm, 2 cm, 3 cm, 4 cm, 5 cm, 6 cm, 7 cm, 8 cm, 9 cm, 10 cm, 11 cm, 12 cm, 13 cm, 14 cm, 15 cm, 16 cm, 17 cm, 18 cm, 19cm, 20 cm, 25 cm, 30 cm, 35 cm, 40 cm, 45 cm, 50 cm, or any integer and decimal value in between.
  • the inner diameter of the outer shell of each bioreactor cartridge of this disclosure is within the range of 1 cm to 500 cm; preferably, 10 cm and 300 cm; more preferably, 30 cm; and 200 cm; and most preferably, 50 cm and 100 cm.
  • the inner diameter of the outer shell of each bioreactor cartridge of this disclosure is within the range of 1cm to 5 cm, 5 cm to 10 cm, 10 cm to 15 cm, 15 cm to 20 cm, 20 cm to 25 cm, 25 cm to 30 cm, 30 cm to 35 cm, 35 cm to 40 cm, 40 cm to 45 cm, 45 cm to 50 cm, 50 cm to 55 cm, 55 cm to 60 cm, 60 cm to 65 cm, 65 cm to 70 cm, 70 cm to 75 cm, 75 cm to 80 cm, 80 cm to 85 cm, 85 cm to 90 cm, 90 cm to 95 cm, 95 cm to 100 cm, 100 cm to 105 cm, 105 cm to 110 cm, 110 cm to 115 cm, 115 cm to 120 cm, 120 cm to 125 cm, 125 cm to 130 cm, 130 cm to 135 cm, 135 cm to 140 cm, 140 cm to 145 cm, 145 cm to 150 cm, 150 cm to 155 cm, 155 cm to 160 cm, 160 cm to 165 cm, 165 cm to 170 cm, 170 cm to 175 cm,
  • Cartridges of assemblies for petrochemical polymer hollow fibres are well known to the art as disclosed in patents US 6, 001 , 585, A and US 2012/0308531.
  • hollow fibres of this disclosure or cartridges thereof are not limited in their use to any specific bioreactor configuration.
  • media supply and process control are adequate to enable confluent cell cultivation.
  • cartridges of the hollow fibres of this disclosure can be used with existing commercial reactor platforms that include, but are not limited to the Terumo BCT Quantum®, FiberCell® Systems Duet Pump, Sartorius Sartoflow®, and systems by Cell Culture Company (HF PrimerTM, AlutovaxID®, AcuSyst-Maximizer®, or AcuSyst-XcelleratorTM).
  • existing commercial reactor platforms that include, but are not limited to the Terumo BCT Quantum®, FiberCell® Systems Duet Pump, Sartorius Sartoflow®, and systems by Cell Culture Company (HF PrimerTM, AlutovaxID®, AcuSyst-Maximizer®, or AcuSyst-XcelleratorTM).
  • cartridges of the hollow fibres of this disclosure can be used in a bioreactor platform that support a single cartridge.
  • cartridges of the hollow fibres of this disclosure can be used in a bioreactor platform that support multiple cartridges.
  • a bioreactor platform capable of supporting cell cultivation with the hollow fibres of this disclosure or cartridges made thereof may have an enclosure of a size in which a single or multiple cartridges may be enclosed.
  • the platform may regulate environmental conditions within the enclosure, such that the temperature, humidity, and gas composition of the enclosed atmosphere are controlled.
  • the cartridges within the platform may be connected with an inlet feed of culture media.
  • the inlet feed line may pass through a pump, such that the rate of the inlet feed may be regulated.
  • the outlet of the cartridge may be connected to a waste reservoir. Alternatively, the outlet of the cartridge may be connected the inlet, such that a recycle of media is established.
  • the platform may be fitted with sensors to detect and measure various cultivation process parameters including, but not limited to, temperature, pH, flow rates, system weights, and the concentration of dissolved metabolites (e.g., oxygen, carbon dioxide, glucose, lactic acid, etc.,).
  • a control system may be fitted to automate the operation of the bioreactor platform and be connected to the sensor array.
  • Such a control system may be controlled locally via the use of a human machine interface (HMI) and/or remotely by installation into a wider control and monitoring network (e.g, SCADA or DeltaV).
  • the bioreactor platform may comprise one or more ports through materials may be inserted or withdrawn.
  • a bioreactor platform capable of supporting cell cultivation with the hollow fibres of this disclosure, or cartridges made thereof has an enclosure of a size in which a single or multiple cartridges may be enclosed.
  • the bioreactor platform regulates environmental conditions within the enclosure, such that the temperature, humidity, and gas composition of the enclosed atmosphere are controlled.
  • each cartridge is connected to one or more inlet feeds of culture media.
  • the inlet feed line(s) pass(es) through one or more pumps, such that the rate of the inlet feed may be regulated.
  • each cartridge is connected to one or more inlets fed from one or more fresh culture media reservoirs.
  • the outlet of each cartridge is connected to one or more waste reservoirs.
  • the outlet of each cartridge is connected to the inlet of the same cartridge, such that a recycle of media is established.
  • the outlet of each cartridge is connected one or more waste reservoirs and to the inlet of the same cartridge, such that a partial recycle of media is established.
  • the outlet of each cartridge is connected to one or more waste reservoirs and intermediate reservoirs.
  • the proportion of outlet flow from the cartridge to the intermediate reservoir(s) is dictated by a recycle ratio.
  • the cartridges are connected to one or more inlets fed from one or more intermediate culture media reservoirs. Each inlet feed is fed with a mixture of fresh and recycled media, with proportions dictated by one or more recycle ratios.
  • the bioreactor platform is fitted with sensors to detect and measure temperature.
  • the bioreactor platform is fitted with sensors to detect and measure pH.
  • the bioreactor platform is fitted with sensors to detect and measure flow rates.
  • the bioreactor platform is fitted with sensors to detect and measure system weights.
  • the bioreactor platform is fitted with sensors to detect and measure the concentration(s) of dissolved metabolite(s) (e.g., oxygen, carbon dioxide, glucose, lactic acid, etc.,).
  • concentration(s) of dissolved metabolite(s) e.g., oxygen, carbon dioxide, glucose, lactic acid, etc.
  • the bioreactor platform is fitted with a control system.
  • the bioreactor platform is fitted with a control system to partially automate the operation of the bioreactor platform.
  • the bioreactor platform is fitted with a control system to automate the operation of the bioreactor platform.
  • the bioreactor platform is fitted with a control system with a sensor array.
  • the bioreactor platform is fitted with a control system that can be controlled locally via the use of a human machine interface (HMI).
  • HMI human machine interface
  • the bioreactor platform is fitted with a control system that can be controlled locally via the use of a human machine interface (HMI) and/or remotely by installation into a wider control and monitoring network (e.g, SCADA or DeltaV).
  • SCADA human machine interface
  • the bioreactor platform is connected to a cell retention device, such as, but not limited to, the XCell® ATF 6.
  • the bioreactor platform comprises one or more ports through materials may be inserted or withdrawn.
  • the inner and/or outer surfaces of hollow fibres of this disclosure are capable of acting as substrates for the adherence and growth of cells, particularly in the production of a cultured meat product.
  • the type of cells cultivated on the hollow fibres of this disclosure include, but are not limited to, satellite cells, mesenchymal stem cells, induced pluripotent stem cells, myocytes, fibroblasts, adipocytes or engineered cells.
  • cultured cells which are suitable for use in the production of cultured meat products include, but are not limited to, satellite cells, mesenchymal stem cells, induced pluripotent stem cells, myocytes, fibroblasts, adipocytes or engineered cells derived from animal sources.
  • animal sources include, but is not limited to:
  • mammals cattle, sheep, pig, horse, goat, deer, reindeer, bison, alpaca, llama, moose, elk, camel, wild boar, buffalo, wildebeest, whale, dolphin and/or guinea pig;
  • birds chicken, turkey, duck, goose, quail, peacock, pheasant, guineafowl, ostrich, emu, pigeon, partridge, grouse, snipe, and/or woodcock;
  • cephalopods squid, octopus, cuttlefish, and/or nautilus
  • crustaceans shrimp, crab, lobster, crawfish, prawn, krill, crayfish, mussel, oyster, clam, scallop, and/or cockle;
  • reptiles snakes, turtles, alligators, crocodiles, iguanas, and/or lizards
  • insects grasshopper, cricket, mealworm, beetle, ant, termite, cicada, caterpillar, silkworm, locust, waxworm, hornworm, bamboo worm, scorpion, and/or centipede.
  • engineered cells derived from the DNA of animals which have since become extinct are cultivated on the hollow fibres of this disclosure.
  • animal include, but are not limited to, mammoths (Mammuthus), and/or dodos (Raphus).
  • the cells cultivated on the hollow fibres of this disclosure are derived from mammals including, but not limited to, human, primate, canine, feline and/or murine sources.
  • the cells cultivated on the hollow fibres of this disclosure are not for use in the production of cultured meat products.
  • the type of cells cultivated on the hollow fibres of this disclosure are all the same, thereby constituting a homo-culture.
  • the type of cells cultivated on the hollow fibres of this disclosure are different, thereby constituting a co-culture.
  • a co-culture of cells is embodied by the concurrent culture of more than one cell type in a single bioreactor.
  • cells cultivated on the hollow fibres of this disclosure are derived from multiple animal sources.
  • cells cultivated on the hollow fibres of this disclosure are derived from multiple sources.
  • the combinations of cell types that can be cultivated together on the hollow fibres of this disclosure in co-cultures include, but are not limited to: myocytes and fibroblasts, myocytes and engineered cells, adipocytes and fibroblasts, adipocytes and engineered cells, myocytes, fibroblasts and adipocytes, myocytes, fibroblasts and engineered cells, myocytes, adipocytes and engineered cells, fibroblasts, adipocytes and engineered cells, or myocytes, fibroblasts, adipocytes and engineered cells.
  • cells derived from non-animal protein sources such as, but not limited to, plants, bacteria, fungi, algae and/or archaea, may be suitably cultivated in bioreactors comprising the hollow fibres of this disclosure and/or cartridges thereof.
  • cells cultivated on the hollow fibres of this disclosure are derived from multiple sources including animals, plants, bacteria, fungi, algae and/or archaea.
  • Edible cells including satellite cells, myocytes and adipocytes, may be sensitive to fluid shear stress; the mechanical force brought about by the friction of fluids flow against the exterior of cell membranes.
  • fluid shear stresses may be high to ensure efficient mass transfer and uniform mixing.
  • hollow fibre bioreactors may be able to maintain very low fluid shear stresses within the extra-capillary spaces, relative to CSTRs (55).
  • Edible cells including satellite cells, myocytes and adipocytes, are typically adherent cell types by nature. During the cultivation of adherent edible cells in CSTRs, cells may be grown on either the surfaces of the bioreactor or upon micro-carriers present within the contained suspension. Alternatively, edible cells may be selectively bred such that they may be cultured in suspension (without microcarriers) or in spheroid aggregates. However, selective breeding programs are typically slow and expensive. Furthermore, the highest cell concentrations achieved in suspension are still usually lower than cell concentrations achieved in hollow fibre bioreactors (55) (4) (2; 3).
  • this disclosure also provides semi-permeable, porous hollow fibres, comprising covalent ester, thioester and/or amide crosslinked polypeptides, to which cells are adhered.
  • this disclosure also provides semi-permeable, porous hollow fibres, comprising covalent ester, thioester and/or amide crosslinked polypeptides, on which cells are adhered and cultured.
  • This disclosure may also provide a food product comprising:

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Abstract

La présente divulgation concerne des fibres creuses poreuses, semi-perméables, extrudées ou filées comprenant des polypeptides réticulés d'ester, de thioester et/ou d'amide covalents ainsi que des procédés de production de celles-ci. Les fibres creuses peuvent être produites à partir de protéines, d'extraits de protéines et/ou d'isolats de protéines dérivés de plantes, d'animaux, de bactéries, d'algues, d'archées et/ou de champignons, et dans certains modes de réalisation, sont destinées à être appropriées pour une ingestion humaine et/ou animale. Dans certains modes de réalisation, les fibres creuses peuvent être conçues pour être utilisées dans la production de cartouches qui sont compatibles avec des plateformes de bioréacteur existantes et/ou nouvelles, destinées à abriter des cultures cellulaires dans la production de viande de culture.
PCT/GB2024/050902 2023-04-06 2024-04-02 Fibres creuses Pending WO2024209200A1 (fr)

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