WO2024246953A1 - Ternary fungicidal composition comprising prothioconazole and strobilurin and other fungicide - Google Patents

Abstract

The present invention provides a synergistic fungicidal compositions comprising first fungicide A, Prothioconazole and second fungicide B, selected from Cyazofamid, Cymoxanil or Dimethomorph; and third fungicide C, selected from Azoxystrobin, Fenaminstrobin, Picoxystrobin, Pyraclostrobin, Pyraoxystrobin or Trifloxystrobin; formulation thereof and to a method of controlling undesired fungi-pests in economically important crops.

Description

TERNARY FUNGICIDAL COMPOSITION COMPRISING PROTHIOCONAZOLE

ANDSTROBILURIN AND OTHER FUNGICIDE

FIELD OF THE INVENTION

The present invention relates to a synergistic fungicidal compositions comprising first fungicide A, Prothioconazole and second fungicide B, selected from Cyazofamid, Cymoxanil or Dimethomorph; and third fungicide C, selected from Azoxystrobin, Fenaminstrobin,

5 Picoxystrobin, Pyraclostrobin, Pyraoxystrobin or Trifloxystrobin; formulation thereof and to a method of controlling undesired fungi-pests in economically important crops.

BACKGROUND OF THE INVENTION

Diseases caused by fungi are a common occurrence on plants, often having a significant economic impact on yield and quality, thus managing fungal diseases is an essential component0 of production for most crops. Fungi often spoil (render unusable) crops in fields, stored fruits, vegetables, tubers, and seeds. Attempts have been made to control the fungal diseases on plants by using single or mixed fungicide active ingredients. Practical agricultural experience has shown that the repeated and exclusive application of an individual active compound in the control of harmful fungi leads in many cases to a rapid selection of those fungus strains which5 have developed natural or adapted resistance against the active compound in question. Effective control of these fungi with the active compound in question is very difficult in such cases.

To reduce the risk of the selection of resistant fungus strains, mixtures of different active compounds are nowadays conventionally employed for controlling harmful fungi. By0 combining active compounds having different mechanisms of action, it is possible to ensure successful control of fungi over a relatively long period of time.

However to minimize the cost of efficient integrated fungi-pest management programs, easy availability of fungicides and to delay the fungi-pest resistance it is necessary to develop a novel fungicidal compositions showing a broader scope of activity and a synergistic effect in5 order to avoid or to control the development of resistant strains to the active ingredients or to the mixtures of known active ingredients used by the farmer while minimising the doses of chemical products sprayed in the agriculture fields and reducing the cost of the treatment. A need also exists for synergistic compositions which shall be physico-chemically compatible formulations in the form of storage stable, safely packed and ready to use formulation. As a solution to the above mentioned problems, the inventors of the present invention surprisingly found that compositions comprising first fungicide A, Prothioconazole and second fungicide B, selected from Cyazofamid, Cymoxanil or Dimethomorph; and third fungicide C, selected from Azoxystrobin, Fenaminstrobin, Picoxystrobin, Pyraclostrobin, Pyraoxystrobin or Trifloxystrobin provides effective control of a wide spectrum of fungal diseases.

SUMMARY OF THE INVENTION

Accordingly, the present invention provides a compositions comprising first fungicide A, Prothioconazole and second fungicide B, selected from Cyazofamid, Cymoxanil or Dimethomorph; and third fungicide C, selected from Azoxystrobin, Fenaminstrobin, Picoxystrobin, Pyraclostrobin, Pyraoxystrobin or Trifloxystrobin and agriculturally acceptable excipient(s).

In one aspect, the compositions of the present invention comprises first fungicide Prothioconazole in an amount in the range from 5 to 30 % w/w; and second fungicides selected from Cyazofamid in an amount in the range from 0.1 to 10 % w/w or Cymoxanil in an amount in the range from 2 to 13 % w/w or Dimethomorph in an amount in the range from 15 to 35 % w/w; and third fungicides selected from Azoxystrobin in an amount in the range from 2 to 15% w/w or Fenaminstrobin in an amount in the range from 2 to 15% w/w or Picoxystrobin in an amount in the range from 4 to 24% w/w or Pyraclostrobin in an amount in the range from 2 to 12% w/w or Pyraoxystrobin in an amount in the range from 1 to 12% w/w or Trifloxystrobin in an amount in the range from 0.1 to 5% w/w and agriculturally acceptable excipient(s).

In one other aspect, the compositions of the present invention comprises first fungicide Prothioconazole in an amount in the range from 10 to 25 % w/w; and second fungicides selected from Cyazofamid in an amount in the range from 2 to 7% w/w or Cymoxanil in an amount the range from 5 to 10 % w/w or Dimethomorph in an amount in the range from 20 to 30 % w/w; and third fungicides selected from Azoxystrobin in an amount in the range from 5 to 10% w/w or Fenaminstrobin in an amount in the range from 5 to 10% w/w or Picoxystrobin in an amount in the range from 10 to 18% w/w or Pyraclostrobin in an amount in the range from 6 to 8% w/w or Pyraoxystrobin in an amount in the range from 4.5 to 8% w/w or Trifloxystrobin in an amount in the range from 1 to 3% w/w. In another aspect, the compositions of the present invention is selected from a group comprising: a) Prothioconazole is present in an amount in the range from 10 to 25% w/w and Cyazofamid is present in an amount in the range from 2 to 7% w/w and Azoxy strobin is present in an amount in the range from 5 to 10% w/w or Fenaminstrobin is present in an amount in the range from 5 to 10% w/w or Picoxystrobin is present in an amount in the range from 10 to 18% w/w or Pyraclostrobin is present in an amount in the range from 6 to 8% w/w or Pyraoxystrobin is present in an amount in the range from 4.5 to 8% w/w or Trifloxystrobin is present in an amount in the range from 1 to 3% w/w and agriculturally acceptable excipients; b) Prothioconazole is present in an amount in the range from 10 to 25% w/w and Cymoxanil is present in an amount in the range from 5 to 10% w/w and Azoxystrobin is present in an amount in the range from 5 to 10% w/w or Fenaminstrobin is present in an amount in the range from 5 to 10% w/w or Picoxystrobin is present in an amount in the range from 10 to 18% w/w or Pyraclostrobin is present in an amount in the range from 6 to 8% w/w or Pyraoxystrobin is present in an amount in the range from 4.5 to 8% w/w or Trifloxystrobin is present in an amount in the range from 1 to 3% w/w and agriculturally acceptable excipients; c) Prothioconazole is present in an amount in the range from 15 to 25% w/w and

Dimethomorph is present in an amount in the range from 20 to 30% w/w and

Fenaminstrobin is present in an amount in the range from 7.5 to 10% w/w or

Pyraoxystrobin is present in an amount in the range from 5 to 8% w/w and agriculturally acceptable excipients;

In another aspect, the present invention provides a process of preparation of the compositions comprising first fungicide A, Prothioconazole and second fungicide B, selected from Cyazofamid, Cymoxanil or Dimethomorph; and third fungicide C, selected from Azoxystrobin, Fenaminstrobin, Picoxystrobin, Pyraclostrobin, Pyraoxystrobin or Trifloxystrobin and agriculturally acceptable excipient(s).

In an aspect, the compositions of the present invention are formulated as Suspension concentrate (SC), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Flowable concentrate for seed treatment (FS), Oil-dispersion (OD), Water dispersible granules (WDG/WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), preferably Suspension concentrate (SC), and Wettable powder (WP).

In another aspect, the compositions of the present invention comprises one or more agriculturally acceptable excipient selected from the group comprising of dispersing agent(s), wetting agent(s), dispersing cum wetting agent(s), disintegrating agent(s), defoaming agent(s) / defoamer(s) / antifoaming agent(s) / antifoamer(s), biocide(s)/preservative(s), viscosity modifier(s)/ rheology modifier(s)/ thickener(s), anti-freeze agent(s), pH modifier(s), pH stabilizer(s), anti-caking agent(s), solvent(s), filler(s), diluent(s) or a combination thereof and other excipients as will be required in a particular type of formulation.

In another aspect, the compositions of the present invention comprises; the dispersing agent, is selected from the group comprising polymethyl methacrylatepolyethylene glycol graft copolymer, acrylate copolymer liquid dispersant, condensed methyl naphthalene sulfonate sodium salt, acrylate copolymer grafted with PEG, polyoxy ethylene alkyl aryl phosphate amine salt, poly-carboxylate dispersant, sodium polycarboxylate, modified styrene acrylic polymeric dispersant, sodium lignosulphonate, sodium salt of naphthalene sulfonate condensate or a combination thereof, and present in an amount in the range from 0.1 to 20% w/w; the wetting agent, is selected from the group comprising polyoxyethylene alkyl ether, sodium alkyl naphthalene sulfonate blend, Adsee 2610 (non-ionic proprietary blend of supplier), polyalkylene oxide block copolymer, ethoxylated tristyrylphenol, sodium isopropyl naphthalene sulfonate, sodium butyl naphthalene sulfonate, sodium dioctylsulphosuccinate, or a combination thereof and present in an amount in the range from 0.1-10% w/w; the dispersing cum wetting agent, is selected from the group comprising proprietary blend of phosphate ester and sodium naphthalene sulphate formaldehyde condensate, Jeemox SCR (proprietary blend of supplier), modified styrene acrylic polymeric dispersant or a combination thereof and present in an amount in the range from 2-10% w/w; the anti-freeze agent, is selected from the group comprising glycerol, propylene glycol, diethylene glycol, monoethylene glycol or a combination thereof and present in an amount in the range from 1 to 10% w/w; the antifoamer/defoamer, is selected from the group comprising polydimethylsiloxane antifoam emulsion, silicon emulsion, polysiloxane emulsion or a combination thereof and present in an amount in the range from 0.01 to 2% w/w; the biocide/preservative, is selected from the group comprising 20% aqueous dipropylene glycol solution of 1, 2-benzisothiazolin-3-one, formaldehyde, isothiazolinone or a combination thereof and present in an amount in the range from 0.01 to 1% w/w; the thickener, is selected from the group comprising xanthan gum, welan gum, guar gum, polyvinyl alcohol, carboxymethylcellulose, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic polymer, starch derivative, polysaccharide, high purity silica, white carbon or a combination thereof and present in an amount in the range from 0.1-0.5% w/w; the pH stabilizer is citric acid and present in an amount in the range from 0.1 to 1.0% w/w; the anticaking agent is selected from the group comprising precipitated silica, organic bentonite, polyolefin particles, fumed silica or a combination thereof, and present in an amount in the range from 1 to 3% w/w; the filler, is selected from the group comprising, silica, precipitated silica, sodium bicarbonate, china clay, lactose, bentonite clay, kaolin, diatomaceous earth or a combination thereof and present in an amount quantity sufficient; and the diluent is demineralised water and present in an amount quantity sufficient.

DETAILED DESCRIPTION OF THE INVENTION

The definitions provided herein for the terminologies used in the present disclosure are for illustrative purpose only and in no manner limit the scope of the present invention disclosed in the present disclosure.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by a person of ordinary skill in the art to which the invention pertains. Although other methods and materials similar, or equivalent, to those described herein can be used in the practice of the present invention, the preferred materials and methods are described herein. It is to be noted that, as used in the specification and the appended claims, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to a composition containing “a compound” includes a mixture of two or more compounds. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise.

As used herein, the terms "comprises", "comprising", "includes", "including", “has”, “having”, “contains”, “containing”, “characterized by” or any other variation thereof, are intended to cover a non-exclusive inclusion, subject to any limitation explicitly indicated. For example, a composition, mixture, process or a method that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, mixture, process or method.

The transitional phrase “consisting of’ excludes any element, step or ingredient not specified. If in the claim, such would close the claim to the inclusion of materials other than those recited except for impurities ordinarily associated therewith. When the phrase “consisting of’ appears in a clause of the body of a claim, rather than immediately following the preamble, it limits only the element set forth in that clause; other elements are not excluded from the claim as a whole.

As used herein, the terms “composition” or “compositions” or "formulation" or "formulations" can be used interchangeably, unless stated otherwise, is meant to encompass, and are not limited to, compositions or formulations containing the combination of first fungicide A, Prothioconazole and second fungicide B, selected from Cyazofamid, Cymoxanil or Dimethomorph; and third fungicide C, selected from Azoxystrobin, Fenaminstrobin, Picoxystrobin, Pyraclostrobin, Pyraoxystrobin or Trifloxystrobin.

As used herein, the term “agriculturally acceptable excipient (s)” refers to inert substances and are auxiliary substances added to the processing or use of fungicide preparations and used to improve the physical and chemical properties of formulations. The auxiliaries themselves are essentially free of biological activity, but can affect the control effect.

The expression of various quantities in terms of “% w/w” or “%” means the percentage by weight, relative to the weight of the total solute on or composition unless otherwise specified. The term “active ingredient” (a.i.) or “active agent” used herein refers to that component of the composition responsible for control of fungi-pests in crops of useful plants.

As used herein, the term "effective amount" means the amount of the active substances in the compositions to achieve an observable effect on death, retardation, prevention, and removal, destruction, or otherwise diminishing the occurrence and activity of the target fungi-pests in an amount not significantly toxic to the crops being treated. An effective amount of the compositions will also vary according to the prevailing conditions such as desired fungicidal effect and duration, weather, target fungi-pests and species, target crops, locus, mode of application, and the like.

As used herein, the term “fungicides”, refers to any chemical substance used to destroy/kill, inhibit or otherwise adversely affect the fungi-pests.

As used herein, the term “Prothioconazole” encompasses prothioconazole or its agrochemically acceptable salt(s), derivative(s), polymorph(s) or any other modified form of prothioconazole belongs to the class of triazolinthione fungicides. It acts as a sterol biosynthesis inhibitor. It is used to control Psuedocercosporella herpotrichoid.es, Fusarium spp., Microdochium nivale, Septoria tritici, Leptosphaeria nodorum, Pyrenophora spp., Rhynchosporium secalis, Puccinia spp. and Blumeria graminis, in wheat, barley and other crops by foliar application and control of Ustilago spp., Tilletia spp., Fusarium spp., and Microdochium nivale as a seed treatment.

As used herein, the term “Cyazofamid” encompasses cyazofamid or its agrochemically acceptable salt(s), derivative(s), polymorph(s) or any other modified form of cyazofamid and belongs to the class of cyanimidazole fungicides. It is used mainly for controlling Oomycete and Plasmodiophora diseases in potatoes, tomatoes, peppers and other vegetables and downy mildew in grapes, cucurbits, melons and other crops.

As used herein, the term “Cymoxanil” encompasses cymoxanil or its agrochemically acceptable salt(s), derivative(s), polymorph(s) or any other modified form of cymoxanil and belongs to the class of cyanoacetaamide oxime fungicides. It is used mainly for controlling Peronospora, Phytophthora and Plasmopara spp. in crops such as vines, hops, potatoes, tomatoes, curcurbits and lettuce. As used herein, the term “Dimethomorph” encompasses dimethomorph or its agrochemically acceptable salt(s), derivative(s), polymorph(s) or any other modified form of dimethomorph and belongs to the class of cinnamamide fungicides. It acts by inhibition of phospholipid biosynthesis and cell wall synthesis. It is used to control of Oomycetes, especially Peronosporaceae and Phytophthora spp. in vines, potatoes, tomatoes, and other crops.

Strobilurin class of fungicides such as Azoxystrobin, Fenaminstrobin, Picoxystrobin, Pyraclostrobin, Pyraoxystrobin, Trifloxystrobin etc are widely used for controlling fungus diseases and are a part of the larger group of Qol (quinone outside inhibition) and acts as a Complex III or MET III electron transport inhibition.

As used herein, the term “Azoxystrobin” encompasses azoxystrobin or its agrochemically acceptable salt(s), derivative(s), polymorph(s) or any other modified form of azoxystrobin and belongs to the class of methoxyacrylate fungicides. It has the broadest spectrum of any antifungal treatment and effective against all four major groups of fungi - the Ascomycota, Deuteromycota, Basidiomycota and the Oomycota.

As used herein, the term “Fenaminstrobin” encompasses fenaminstrobin or its agrochemically acceptable salt(s), derivative(s), polymorph(s) or any other modified form of fenaminstrobin and belongs to class of oximinoacetamide fungicides and works as a broad-spectrum fungicide.

As used herein, the term “Picoxystrobin” encompasses picoxystrobin or its agrochemically acceptable salt(s), derivative(s), polymorph(s) or any other modified form of picoxystrobin and belongs to the class of methoxyacrylate fungicides. It is a preventive and curative fungicide and uses on canola, cereals grains, dried peas, com and soybeans.

As used herein, the term “Pyraclostrobin” encompasses pyraclostrobin or its agrochemically acceptable salt(s), derivative(s), polymorph(s) or any other modified form of pyraclostrobin and belongs to the class of methoxycarbamate fungicides. It is a fungicide with protectant, curative and translaminar properties. It is used to control plant pathogens in cereals, peanuts, soybeans, grapes, potatoes, tomatoes, vegetables, banana, citrus, turf.

As used herein, the term “Pyraoxystrobin” encompasses pyraoxystrobin or its agrochemically acceptable salt(s), derivative(s), isomer(s), polymorph(s) or any other modified form of pyraoxystrobin and belongs to the class of methoxyacrylate fungicides. It is a broad spectrum fungicide used to control a variety of diseases like downey mildew, powdery mildew, Botrytis cinerea, Bipolaris oryzae, Alternmaria solani etc. in rice, vegetables, tomatoes and tea.

As used herein, the term “Trifloxystrobin” encompasses trifloxystrobin or its agrochemically acceptable salt(s), derivative(s), polymorph(s) or any other modified form of Trifloxystrobin and belongs to class of oximinoacetate fungicides. It used as broad spectrum fungicide with preventative and curative action in cereals, soybeans, maize, rice, cotton, peanuts, sugar beet, sunflower, fruit and ornamental, turf and many vegetables.

In an embodiment, the present invention provides a compositions comprising first fungicide A, Prothioconazole and second fungicide B, selected from Cyazofamid, Cymoxanil or Dimethomorph; and third fungicide C, selected from Azoxystrobin, Fenaminstrobin, Picoxystrobin, Pyraclostrobin, Pyraoxystrobin or Trifloxystrobin and agriculturally acceptable excipient(s).

In an embodiment, the present invention provides a compositions comprises first fungicide Prothioconazole in an amount in the range from 5 to 30 % w/w; and second fungicides selected from Cyazofamid in an amount in the range from 0.1 to 10 % w/w or Cymoxanil in an amount in the range from 2 to 13 % w/w or Dimethomorph in an amount in the range from 15 to 35 % w/w; and third fungicides selected from Azoxystrobin in an amount in the range from 2 to 15% w/w or Fenaminstrobin in an amount in the range from 2 to 15% w/w or Picoxystrobin in an amount in the range from 4 to 24% w/w or Pyraclostrobin in an amount in the range from 2 to 12% w/w or Pyraoxystrobin in an amount in the range from 1 to 12% w/w or Trifloxystrobin in an amount in the range from 0.1 to 5% w/w and agriculturally acceptable excipient(s).

In an embodiment, the compositions of the present invention comprises first fungicide Prothioconazole in an amount in the range from 10 to 25 % w/w; and second fungicides selected from Cyazofamid in an amount in the range from 2 to 7% w/w or Cymoxanil in an amount the range from 5 to 10 % w/w or Dimethomorph in an amount in the range from 20 to 30 % w/w; and third fungicides selected from Azoxystrobin in an amount in the range from 5 to 10% w/w or Fenaminstrobin in an amount in the range from 5 to 10% w/w or Picoxystrobin in an amount in the range from 10 to 18% w/w or Pyraclostrobin in an amount in the range from 6 to 8% w/w or Pyraoxystrobin in an amount in the range from 4.5 to 8% w/w or Trifloxystrobin in an amount in the range from 1 to 3% w/w and agriculturally acceptable excipient(s). In an embodiment, the compositions of the present invention is selected from a group comprising: a) Prothioconazole is present in an amount in the range from 10 to 25% w/w and Cyazofamid is present in an amount in the range from 2 to 7% w/w and Azoxy strobin is present in an amount in the range from 5 to 10% w/w or Fenaminstrobin is present in an amount in the range from 5 to 10% w/w or Picoxystrobin is present in an amount in the range from 10 to 18% w/w or Pyraclostrobin is present in an amount in the range from 6 to 8% w/w or Pyraoxy strobin is present in an amount in the range from 4.5 to 8% w/w or Trifloxystrobin is present in an amount in the range from 1 to 3% w/w and agriculturally acceptable excipients; b) Prothioconazole is present in an amount in the range from 10 to 25% w/w and Cymoxanil is present in an amount in the range from 5 to 10% w/w and Azoxystrobin is present in an amount in the range from 5 to 10% w/w or Fenaminstrobin is present in an amount in the range from 5 to 10% w/w or Picoxystrobin is present in an amount in the range from 10 to 18% w/w or Pyraclostrobin is present in an amount in the range from 6 to 8% w/w or Pyraoxy strobin is present in an amount in the range from 4.5 to 8% w/w or Trifloxystrobin is present in an amount in the range from 1 to 3% w/w and agriculturally acceptable excipients; c) Prothioconazole is present in an amount in the range from 15 to 25% w/w and Dimethomorph is present in an amount in the range from 20 to 30% w/w and Fenaminstrobin is present in an amount in the range from 7.5 to 10% w/w or Pyraoxy strobin is present in an amount in the range from 5 to 8% w/w and agriculturally acceptable excipients;

In an embodiment, the compositions of the present invention is selected from a group comprising: a) Prothioconazole is present in an amount in the range from 10 to 20% w/w and Cyazofamid is present in an amount in the range from 2.5 to 6.0% w/w and Azoxystrobin is present in an amount in the range from 6 to 10% w/w and agriculturally acceptable excipients; b) Prothioconazole is present in an amount in the range from 15 to 20% w/w and Cyazofamid is present in an amount in the range from 2.0 to 6.0% w/w and Fenaminstrobin is present in an amount in the range from 5 to 9% w/w and agriculturally acceptable excipients; c) Prothioconazole is present in an amount in the range from 12 to 20% w/w and Cyazofamid is present in an amount in the range from 2.5 to 6.5% w/w and Picoxystrobin is present in an amount in the range from 10 to 15% w/w and agriculturally acceptable excipients; d) Prothioconazole is present in an amount in the range from 12 to 20% w/w and Cyazofamid is present in an amount in the range from 2 to 6% w/w and Pyraclostrobin is present in an amount in the range from 6 to 8% w/w and agriculturally acceptable excipients; e) Prothioconazole is present in an amount in the range from 15 to 25% w/w and Cyazofamid is present in an amount in the range from 2.5 to 6.5% w/w and Pyraoxystrobin is present in an amount in the range from 4.5 to 8% w/w and agriculturally acceptable excipients; f) Prothioconazole is present in an amount in the range from 15 to 25% w/w and Cyazofamid is present in an amount in the range from 3 to 7% w/w and Trifloxystrobin is present in an amount in the range from 1 to 3% w/w and agriculturally acceptable excipients; g) Prothioconazole is present in an amount in the range from 10 to 20% w/w and Cymoxanil is present in an amount in the range from 5 to 8% w/w and Azoxystrobin is present in an amount in the range from 5 to 10% w/w and agriculturally acceptable excipients; h) Prothioconazole is present in an amount in the range from 15 to 20% w/w and Cymoxanil is present in an amount in the range from 5 to 8% w/w and Fenaminstrobin is present in an amount in the range from 5 to 9% w/w and agriculturally acceptable excipients; i) Prothioconazole is present in an amount in the range from 12 to 20% w/w and Cymoxanil is present in an amount in the range from 6 to 10% w/w and Picoxystrobin is present in an amount in the range from 10 to 18% w/w and agriculturally acceptable excipients; j) Prothioconazole is present in an amount in the range from 12 to 20% w/w and Cymoxanil is present in an amount in the range from 6 to 8% w/w and Pyraclostrobin is present in an amount in the range from 6 to 8% w/w and agriculturally acceptable excipients; k) Prothioconazole is present in an amount in the range from 15 to 25% w/w and Cymoxanil is present in an amount in the range from 5.5 to 9% w/w and Pyraoxystrobin is present in an amount in the range from 5.5 to 8% w/w and agriculturally acceptable excipients; 1) Prothioconazole is present in an amount in the range from 12 to 20% w/w and Cymoxanil is present in an amount in the range from 6 to 8% w/w and Trifloxystrobin is present in an amount in the range from 1 to 3% w/w and agriculturally acceptable excipients; m) Prothioconazole is present in an amount in the range from 15 to 20% w/w and Dimethomorph is present in an amount in the range from 20 to 30% w/w and Fenaminstrobin is present in an amount in the range from 7.5 to 10% w/w and agriculturally acceptable excipients; n) Prothioconazole is present in an amount in the range from 15 to 25% w/w and Dimethomorph is present in an amount in the range from 20 to 30% w/w and Pyraoxystrobin is present in an amount in the range from 5 to 8% w/w and agriculturally acceptable excipients; In another embodiment, the compositions of the present invention are formulated as Suspension concentrate (SC), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Flowable concentrate for seed treatment (FS), Oil-dispersion (OD), Water dispersible granules (WDG/WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT) preferably Suspension concentrate (SC), and Wettable powder (WP).

In one other embodiment, the compositions of the present invention comprises one or more agriculturally acceptable excipient selected from the group comprising of dispersing agent(s), wetting agent(s), dispersing cum wetting agent(s), disintegrating agent(s), defoaming agent(s) / defoamer(s) / antifoaming agent(s) / antifoamer(s), biocide(s)/preservative(s), viscosity modifier(s)/ rheology modifier(s)/ thickener(s), anti-freeze agent(s), pH modifier(s), pH stabilizer(s), anti-caking agent(s), solvent(s), filler(s), diluent(s) or a combination thereof and other excipients as will be required in a particular type of formulation.

The compositions of present invention uses dispersing agents to prevent agglomeration of solid particles and keep them suspended in fluid. Non-limiting examples of dispersing agent that can be used in the present invention include, but not limited to polymethyl methacrylatepolyethylene glycol graft copolymer, acrylate copolymer liquid dispersant, condensed methyl naphthalene sulfonate sodium salt, acrylate copolymer grafted with PEG, polyoxy ethylene alkyl aryl phosphate amine salt, poly-carboxylate dispersant, sodium polycarboxylate, modified styrene acrylic polymeric dispersant, sodium lignosulphonate, sodium salt of naphthalene sulfonate condensate, ethoxylated polyarylphenol phosphate amine salt, polymethyl methacrylate-polyethylene oxide graft copolymer, graft copolymer of polymethyl methacrylate -polyethylene glycol, ethoxylated isodecyl alcohol, blend of acrylic graft copolymer and emulsifier, tristyrylphenol ethoxylate, anionic tristyrylphenol phosphate surfactant, non-ionic surfactant, poly alkoxy lated butyl ether dispersant, sodium alkylnaphthalene sulfonate condensate, sodium naphthalene sulphonate salt, sodium alkylnaphthalene sulfonate phenol condensate, sodium alkylnaphthalene sulfonate formaldehyde condensate, modified styrene acrylic polymeric aqueous dispersant, proprietary blend of phosphate ester and sodium naphthalene sulphate formaldehyde condensate, lignosulfonic acid dispersant, kraft ligno sulfonate with a low degree sulfonation, modified sodium ligno sulphonate, naphthalene sulfonate formaldehyde condensate, mixture of salt of naphthalene sulphonic acid and phenol, alkyl naphthalene sulfonate sodium salt, modified naphthalene sulfonate formaldehyde condensate, highly sulfonated kraft lignin, naphthalene sulfonate phenol condensate, sodium isopropyl naphthalene sulfonate. One or more dispersing agents may be used in the compositions of the present invention. Dispersing agents is present in an amount in the range from 0.1 to 20% w/w;

The compositions of present invention uses wetting agent to facilitate the process of dispersion of the granules in the liquid. Non-limiting examples of wetting agents that can be used in the present invention include, but not limited to polyoxyethylene alkyl ether, sodium alkyl naphthalene sulfonate blend, Adsee 2610 (non-ionic proprietary blend of supplier), polyalkylene oxide block copolymer, ethoxylated tristyrylphenol, sodium isopropyl naphthalene sulfonate, sodium butyl naphthalene sulfonate, sodium dioctylsulphosuccinate, branched short chain alcohol ethoxylate wetter, sodium alkyl naphthalene sulfonate with an anionic blend, polyalkylene glycol ether, EO/PO block co-polymer, sodium alkyl naphthalene sulfonate blend with a anionic blend, nonionic proprietary surfactant blend, sulphonated anionic wetter, copolymer condensate of ethylene and propylene oxide, ethoxylated isodecyl alcohol, sodium alkyl naphthalene sulfonate formaldehyde condensate, comb-type polymeric surfactant, ethoxylated polyarylphenol phosphate amine salt, dibutyl naphthalene sulfonic acid sodium salt, dialkyl naphthalene sulphonate. One or more wetting agent may be used in the compositions of the present invention. Wetting agent is present in an amount in the range from 0.1-10% w/w;

The compositions of present invention also uses dispersing cum wetting agent. Non-limiting examples of dispersing cum wetting agent that can be used in the present invention include, but not limited to proprietary blend of phosphate ester and sodium naphthalene sulphate formaldehyde condensate, Jeemox SCR (proprietary blend of supplier), modified styrene acrylic polymeric dispersant, highly sulfonation and low molecular weight kraft lignin polymer, sodium salt of aromatic ring surfactant, ammonium salt of aromatic ring surfactant, sodium salt of naphthalene sulphonic acid condensation, proprietary blend of anionic surfactant, proprietary blend of polymeric surfactant, sodium methyl oleoyl taurate, tristyrylphenol ethoxylate phosphate ester. One or more dispersing cum wetting agent may be used in the compositions of the present invention. Dispersing cum wetting agent is present in an amount in the range from 2-10% w/w;

The compositions of present invention uses anti-freeze agent which are used to prevent deleterious effects due to freezing and lyophilization (lyophilisation). Suitable anti-freeze agent used herein, but not limited to glycerol, propylene glycol, diethylene glycol, monoethylene glycol. One or more anti-freeze agent may be used in the compositions of the present invention. Anti-freeze agent is present in an amount in the range from 1 to 10% w/w.

The compositions of present invention also uses defoaming agent. The term “defoaming agent(s)” or “defoamer(s)” or “antifoaming agent(s)” “antifoamer(s)” refers to a chemical additive that reduces and hinders the formation of foam in liquid, semi- solid, or solid formulations. The terms defoaming agent and anti-foaming agent can be used interchangeably. Suitable defoamer used herein, but not limited to, polydimethylsiloxane antifoam emulsion, silicon emulsion, polysiloxane emulsion, organic fluorine compounds. One or more defoaming agent may be used in the compositions of the present invention. Defoaming agent is present in in an amount in the range from 0.01 to 2% w/w.

The compositions of present invention uses biocide/preservatives to prevent the composition from spoilage due to bacteria, yeasts and fungi or deterioration due to undesirable chemical changes. Suitable biocide/preservative used herein, but not limited to 20% aqueous dipropylene glycol solution of 1, 2- benzisothiazolin-3-one, formaldehyde, potassium sorbate, 4- hydroxybenzoic acid esters, 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin- 3-one. One or more biocide/preservatives may be used in the compositions of the present invention. Biocide/preservatives is present in an amount in the range from 0.01-1% w/w.

The compositions of present invention uses thickener which are usually polymeric material, and at a low concentration increases the viscosity of an aqueous solution and helps to stabilize the composition. Suitable thickener used herein, but not limited to, xanthan gum, welan gum, guar gum, polyvinyl alcohol, carboxymethylcellulose, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic polymer, starch derivative, polysaccharide, high purity silica, white carbon. One or more thickener may be used in the compositions of the present invention. Thickener is present in an amount in the range from 0.1-5% w/w.

The compositions of present invention uses pH stabilizer. Suitable pH stabilizer that may be used in the compositions of the present invention is citric acid and present in an amount in the range from 0.1 to 1.0% w/w.

The compositions of present invention uses anticaking agent. Suitable anticaking agent used herein, but not limited to, precipitated silica, organic bentonite, polyolefin particles, fumed silica. One or more anticaking agent may be used in the compositions of the present invention.

Anticaking agent is present in an amount in the range from 1 to 3% w/w;

The compositions of present invention uses filler. Suitable fillers used herein, but not limited to, silica, precipitated silica, sodium bicarbonate, china clay, lactose, bentonite clay, kaolin, diatomaceous earth. One or more filler may be used in the compositions of the present invention. The filler may be used in an amount of quantity sufficient to make 100% w/w formulation.

The compositions of present invention uses diluents. Suitable diluents that may be used in the composition of the present invention is demineralised water and present in an amount quantity sufficient to make 100% w/w formulation.

In an embodiment, the present invention provides a process of preparation of the compositions comprising first fungicide A, Prothioconazole and second fungicide B, selected from Cyazofamid, Cymoxanil or Dimethomorph; and third fungicide C, selected from Azoxystrobin, Fenaminstrobin, Picoxystrobin, Pyraclostrobin, Pyraoxystrobin or Trifloxystrobin and agriculturally acceptable excipient(s).

In an embodiment, the present invention provides a process for the preparation of a suspension concentrate (SC) formulations which comprises: a) diluting and solubilizing a biocide, defoamer and antifreeze agent in demineralised water in a mixing vessel and adding dispersing and wetting agent under stirring to get homogeneous mixture; b) adding active ingredients one by one under stirring and mixing till homogeneous slurry obtained; c) wet grinding at suitable temperature till desired particle size obtained, and d) adding previously prepared viscosity modifier solution and stirring to obtain suspension concentrate formulation.

In an embodiment, the present invention provides a process for the preparation of a wettable powder (WP) formulation which comprises: a) weighing and mixing of required active ingredients and other ingredients; b) milling in a suitable air jet mill; c) grinding the material to obtain desired particle size (D90- <10 micron) and d) mixing homogeneously in a blender to obtain the wettable powder formulation. The compositions of this invention are useful as plant disease control agents. The present invention therefore further comprises a method for controlling plant diseases caused by fungal plant pathogens comprising applying to the plant or plants portions such as seeds, seedlings, saplings, roots, tubers, stems, leaves, stalks, foliage and fruits to be protected an effective amount of one or more of aforesaid fungicidal composition.

The compositions of the present invention can be applied by any one of the methods selected from atomization, spreading, dusting, spraying, diffusion, immersion, irrigation (chemigation), mixing, foaming, dressing, coating, blasting, fumigation, smoking, smog and painting.

The rate of application amount of the prepared compositions varies depending on, for example, the blending ratio of active ingredients, meteorological condition, dosage form, application time, application method, application place, fungi to be controlled, and a target crop, in ordinary cases.

The compositions of present invention provide controls on variety of diseases such as Fruit rot (Anthracnose), Powdery mildew, Damping off, Leaf spot, Die -back, twig blight, Wilt, Stem Rot, Early & late blight, Ring Rot, Buck eye rot, Alternaria leaf spot, Cercospora leaf spot, Blight, Black Scurf, Scab, Anthracnose, Purple blotch, Stemphylium blight, Black mold and neck rot, Collar rot, Rust, Downey mildew, Angular leafspot, Marssonina leaf blotch, Premature leaf fall, Core rot, Greasy spot, Gummosis (Foot Rot) (Phytophthora palmivora), Root rot, Crown rot, Leaf fall and Brown rot, Sooty mould, Anthracnose leaf spot, Sigatoka leaf spot, Fruit rot, Cigar end rot, Tip rot, Leaf and Fruit spots, Downy leafspot, Black Rot, Grey blight, Blister Blight, red rust, Coffee leaf rust, Leaf blotch, Rhizome rot, Sheath blight, Leaf blast, Brown Spot, Sheath rot, neck blast, False smut, Dirty panicle, Grain discoloration, Leafspot, Grey mildew, Alternaria Leaf blight, Turcicum leaf blight, Seed rot, Seedling blight, Browny stripe, Red rot, Smut, White rust, Early leafspot (Cercospora arachidicola), Late leafspot (C. personata), Loose smut, Yellow rust, Karnal bunt, Leaf blight, Stripe rust, flag smut, Myrothecium leaf spot, Fusarium root rot, Phytophthora root rot, Rhizoctonia seedling blight, Pythium seedling blight, Pod & stem blight, Charcoal rot, Frog eye leaf spot, Wilt (Fusarium oxyspor um), Root rot (Rhizoctonia spp.), Seed and Seedling rot disease, Web blight, Grain smut, Fusarium wilt, Alternaria blight, Blackspot etc. in a variety of crops such as Chilli, Okra, Tomato, Brinjal, Potato, Cucumber, Onion, Cabbage, Cauliflower, Beans, Cowpea, Guar, Pea, Cucurbits, Bitter gourd, Sugar beet, Grapes, Mango, Apple, Citrus, Guava, Banana, Pomegranate, Walnut, Ber, Watermelon, Tea, Coffee, Papaya, Turmeric, Rice, Cotton, Maize, Sugarcane, Mustard, Sunflower, Pulses, Pearl millet, Groundnut, Wheat, Soybean, Chickpea, Tobacco, Black gram/Green gram, Sorghum, Pigeon pea, Opium, Cumin, Rose and many other economically important crops.

The inventors of the present invention have surprisingly found that the compositions of the present invention are synergetic in nature. The synergetic compositions of the present invention are more effective than their individual counterparts or when two actives are taken together. The synergetic composition of the present invention also makes it possible to use markedly smaller quantities of the active ingredients as compared to their individual counterparts. This allows a substantial reduction in the application rates of each of these active ingredients, while maintaining good efficacy. The decrease in application rates reduces treatment cost to the farmer and also eases the burden on the environment both from manufacturing waste and crop protection chemical residues.

The fungicidal compositions of present invention provides a wide spectrum control of fungi- pest and minimizes the risk of development of resistance ultimately, which reflects the delays in emergence of the resistant strains hence provide effective and economical control of undesired fungi-pest.

The fungicidal compositions of the present invention provides a number of other advantages:

• Significant increased efficacy in comparison to the other formulations tested during the trials.

• Are synergistic hence denies the need of sequential application of solo fungicides to control different fungi spectra thereby reducing the environmental load of fungicides.

• Uses lesser amounts of active ingredients hence considerable reduction in cost, which would benefit farmers.

• The compositions provided one shot solution to farmers to control broad spectrum of fungi-pests.

• Are storage stable.

• Are completely safer to crops (non-phytotoxic).

• Reduction in environmental load of fungicidal residues.

• Reduction in quantity of water required for spray of the product in agriculture fields. • Reduction in generation of empty containers of product, thereby, reducing concerns of their disposal.

• Provides a broad range of product choice for effective pest management.

Thus, from the foregoing description, it will be apparent to one of the person skilled in the art that many changes and modifications can be made thereto without departing from the scope of the invention as set forth in the description. Accordingly, it is not intended that the scope of the foregoing description be limited to the description set forth above, but rather that such description be construed as encompassing such features that reside in the present invention, including all the features and embodiments that would be treated as equivalents thereof by those skilled in the relevant art.

The embodiments of the present invention are more particularly described in the following examples that are intended as illustrations only, since numerous modifications and variations within the scope of the present invention will be apparent to those of skill in the art. Unless otherwise noted, all parts, percentages and ratios reported in the following examples are on a weight basis and all reagents used in the examples were obtained or are available from the chemical suppliers.

EXAMPLES

The Fungicidal compositions of the present invention comprising first fungicide A, Prothioconazole and second fungicide B, selected from Cyazofamid, Cymoxanil or Dimethomorph; and third fungicide C, selected from Azoxystrobin, Fenaminstrobin, Picoxystrobin, Pyraclostrobin, Pyraoxystrobin or Trifloxystrobin and agriculturally acceptable excipient(s) in the form of suspension concentrate (SC) and wettable powder (WP) is provided in table 1-56. The unit of each component of the compositions are expressed in “% w/w” i.e. the percentage by weight, relative to the weight of the total solution or composition. All the compositions are prepared by following the processes as disclosed in description. All the prepared compositions of the present invention has been tested for stability/shelf life at accelerated temperature.

Table 1: Examples 1 to 6: Compositions of Prothioconazole + Cyazofamid + Azoxystrobin

Table 2: Quantities of ingredients in gram charged to prepare the compositions of Eg 1 and 4 of table 1

Table 3: Stability studies of the composition of Eg 1 of table 2

Table 4: Stability studies of the composition of Eg 4 of table 2

Table 5: Examples 7 to 12: Compositions of Prothioconazole + Cyazofamid + Fenaminstrobin

Table 6: Quantities of ingredients in gram charged to prepare the compositions of Eg 7 and 10 of table 5

Table 7: Stability studies of the composition of Eg 7 of table 6

Table 8: Stability studies of the composition of Eg 10 of table 6

Table 9: Examples 13 to 18: Compositions of Prothioconazole + Cyazofamid + Picoxystrobin

Table 10: Quantities of ingredients in gram charged to prepare the compositions of Eg 13 and

16 of table 9

Table 11: Stability studies of the composition of Eg 13 of table 10

Table 12: Stability studies of the composition of Eg 16 of table 10

Table 13: Examples 19 to 24: Compositions of Prothioconazole + Cyazofamid + Pyraclostrobin

Table 14: Quantities of ingredients in gram charged to prepare the compositions of Eg 19 and 22 of table 13

Table 15: Stability studies of the composition of Eg 19 of table 14

Table 16: Stability studies of the composition of Eg 22 of table 14

Table 17: Examples 25 to 30: Compositions of Prothioconazole + Cyazofamid + Pyraoxystrobin

Table 18: Quantities of ingredients in gram charged to prepare the compositions of Eg 25 and 28 of table 17

Table 19: Stability studies of the composition of Eg 25 of table 18

Table 20: Stability studies of the composition of Eg 28 of table 18

Table 21: Examples 31 to 36: Compositions of Prothioconazole + Cyazofamid + Trifloxystrobin

Table 22: Quantities of ingredients in gram charged to prepare the compositions of Eg 31 and 34 of table 21

Table 23: Stability studies of the composition of Eg 31 of table 22

Table 24: Stability studies of the composition of Eg 34 of table 22

Table 25: Examples 37 to 42: Compositions of Prothioconazole + Cymoxanil + Azoxystrobin

Table 26: Quantities of ingredients in gram charged to prepare the compositions of Eg 37 and 40 of table 25

Table 27: Stability studies of the composition of Eg 37 of table 26

Table 28: Stability studies of the composition of Eg 40 of table 26

Table 29: Examples 43 to 48: Compositions of Prothioconazole + Cymoxanil + Fenaminstrobin

Table 30: Quantities of ingredients in gram charged to prepare the compositions of Eg 43 and 46 of table 29

Table 31: Stability studies of the composition of Eg 43 of table 30

Table 32: Stability studies of the composition of Eg 46 of table 30

Table 33: Examples 49 to 54: Compositions of Prothioconazole + Cymoxanil + Picoxystrobin

Table 34: Quantities of ingredients in gram charged to prepare the compositions of Eg 49 and 52 of table 33

Table 35: Stability studies of the composition of Eg 49 of table 34

Table 36: Stability studies of the composition of Eg 52 of table 34

Table 37: Examples 55 to 60: Compositions of Prothioconazole + Cymoxanil + Pyraclostrobin

Table 38: Quantities of ingredients in gram charged to prepare the compositions of Eg 55 and 58 of table 37

Table 39: Stability studies of the composition of Eg 55 of table 38

Table 40: Stability studies of the composition of Eg 58 of table 38

Table 41: Examples 61 to 66: Compositions of Prothioconazole + Cymoxanil + Pyraoxystrobin

Table 42 Quantities of ingredients in gram charged to prepare the compositions of Eg 61 and 64 of table 41

Table 43: Stability studies of the composition of Eg 61 of table 42

Table 44: Stability studies of the composition of Eg 64 of table 42

Table 45: Examples 67 to 72: Compositions of Prothioconazole + Cymoxanil + Trifloxystrobin

Table 46: Quantities of ingredients in gram charged to prepare the compositions of Eg 67 and 70 of table 45

Table 47: Stability studies of the composition of Eg 67 of table 46

Table 48: Stability studies of the composition of Eg 70 of table 46

Table 49: Examples 73 to 78: Compositions of Prothioconazole + Dimethomorph + Fenaminstrobin

Table 50: Quantities of ingredients in gram charged to prepare the compositions of Eg 73 and 76 of table 49

Table 51: Stability studies of the composition of Eg 73 of table 50

Table 52: Stability studies of the composition of Eg 76 of table 50

Table 53: Examples 79 to 84: Compositions of Prothioconazole + Dimethomorph + Pyraoxystrobin

Table 54: Quantities of ingredients in gram charged to prepare the compositions of Eg 79 and 82 of table 53

Table 55: Stability studies of the composition of Eg 82 of table 54

Table 56: Stability studies of the composition of Eg 79 of table 54

As evident from the foregoing tables (1-56) that all the desired compositions have been prepared successfully and has been tested for stability/shelf life at accelerated temperature.

After 14 days of storage at 54.0 ± 2 °C, there were no significant changes in active ingredient content, suspensibility, wettability, dispersibility, pH and has passes the sieve test. Therefore, it is concluded that compositions of present invention has good stability and shelf life. BIO-EFFICACY OF THE FUNGICIDAL COMPOSITIONS OF THE PRESENT INVENTION

Experimental trial were conducted to evaluate the fungicidal compositions of the present invention in India against major fungi-pests on Chilli and Potato Crop with ternary compositions of present invention and comparative solo and binary compositions as per the details provided in Table 57-84. The abbreviations used in Table 57-84 are as follows:

TN: Treatment Number; g.a.i.: gram active ingredients; ha: hectare;

O=Observed % fungi-pest reduction (%) Calculated using Abbott Formula; E=Expected % fungi-pest reduction Calculated using Colby Formula;

CR= Colby ratio;

UTC: Untreated control

Experimental design and method for Chilli Crop

The present field experiment were conducted at Baptala of Guntur district of Andhra Pradesh state during the season Rabi-2022 with twenty-two treatments replicated thrice in randomized block design. The popular and widely cultivated Guntur Chilli variety S-4 selected for this experiment was sown in a plot size of 5m X 5m for each replication with a spacing of 50 X 50cm in sandy loam soil. The crop was maintained well by adapting standard agronomic practices. Total two round of sprays were given, 1^st at 0 days and 2^nd at after 10 days. The observation were recorded on percent disease index and fruit yield. The data on disease incidence were recorded after each application and presented as mean percent PDI of two spray. The first spray of fungicide was done after the first appearances of disease symptoms. Unsprayed plots served as control (Untreated). The applications were carried out by using the hand operated aspire Knapsack sprayer pump fitted with hollow cone nozzle. The required quantity of water volume was used @ 500 litre per hectare. The severity of Chilli die-back disease were recorded on randomly tagged 5 plants per plot by using 0-9 disease rating scale and expressed as Percent Disease index (PDI) (Wheeler, 1969).

The Chilli fruits were harvested periodically and the yield per hectare was calculated and recorded as tones/ha.

Assessment of PDI (Percent Disease Index)

The percent disease index (PDI) was calculated by using the formula given by Wheeler (1969)

Sum of Numerical rating

Percent Disease Index (PDI) = -

Number of plant assessed x Maximum rating The % reduction in target pest over control was calculated by using Abbott's formula (Reference: Abbot, W.S. (1925). A method of computing the effectiveness of an insecticide. J. Econ. Entomol.; 18 : 265-267. n in T after treatment Corrected % = (1 - - ) X 100 n in Co after treatment

Where: n = Percent PDI, T = treated, Co = control

Experimental design and method for Potato Crop

The present field experiment were conducted at Aligarh district of Uttar Pradesh state during the season Rabi-2023 with twenty-two treatments replicated thrice in randomized block design. The popular and widely cultivated Potato variety Kufri Ashoka selected for this experiment was sown in a plot size of 4m X 4m for each replication with a spacing of 60 X 20cm in sandy loam soil. The crop was maintained well by adapting standard agronomic practices. Total two round of sprays were given, 1^st at 0 days and 2^nd at after 10 days. The observation were recorded on disease incidence and fruit yield. The data on disease incidence were recorded after each application and presented as mean percent PDI of two spray. The first spray of fungicide was done after the first appearances of disease symptoms. Unsprayed plots served as control (Untreated). The applications were carried out by using the hand operated aspire Knapsack sprayer pump fitted with hollow cone nozzle. The required quantity of water volume was used @ 500 litre per hectare. The disease severity of late blight of potato were recorded from randomly selected 10 plants per plot and 1 upper leaf, 1 middle leaf and 1 lower leaf of each plant and percent disease index (PDI) is calculated by using the formula given by Wheeler (1969). The % reduction in target pest over control was calculated by using Abbott's formula as given above.

The Potato tuber were harvested periodically and the yield per hectare was calculated and recorded as tones/ha.

Assessment of PDI (Percent Disease Index)

Rating scale for the assessment of late blight severity on potato leaves

Evaluation of synergistic effect

A synergistic effect exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components. Synergism was calculated by using Colby’s method, Weeds, vol. 15 No. l(Jan 1967), pp. 20-2.

The synergistic action expected for a given combination of two active components can be calculated as follows: g=(X+Y) — — —

100

The synergistic action expected for a given combination of three active components can be calculated as follows:

(XY+YZ+XZ) XYZ

E=(X+Y+Z) - + -

100 10000

Where:

E represents expected percentage of control for the combination of the three active ingredients at defined doses (for example equal to x, y and z respectively),

X is the percentage of control observed by the compound (I) at a defined dose (equal to x),

Y is the percentage of control observed by the compound (II) at a defined dose (equal to y),

Z is the percentage of control observed by the compound (III) at a defined dose (equal to z).

If observed control of the combination > Expected control, the combination is synergistic (Colby ratio > 1)

If observed control of the combination < Expected control, the combination is antagonistic (Colby ratio < 1) If observed control of the combination = Expected control, the combination is additive (Colby ratio =1)

Table 57: Efficacy of Fungicidal Compositions of Prothioconazole + Cyazofamid + Azoxystrobin in Potato Crop against Late blight

Table 58: Effect on yield of Fungicidal Compositions of table 57

Table 59: Efficacy of Fungicidal Compositions of Prothioconazole + Cyazofamid + Fenaminstrobin in Potato Crop against Fate blight

Table 61: Efficacy of Fungicidal Compositions of Prothioconazole + Cyazofamid + Picoxystrobin in Potato Crop against Late blight

Table 62: Effect on yield of Fungicidal Compositions of table 61

Table 63: Efficacy of Fungicidal Compositions of Prothioconazole + Cyazofamid + Pyraclostrobin in Potato Crop against Late blight

Table 64: Effect on yield of Fungicidal Compositions of table 63

Table 65: Efficacy of Fungicidal Compositions of Prothioconazole + Cyazofamid + Pyraoxystrobin in Potato Crop against Late blight

Table 66: Effect on yield of Fungicidal Compositions of table 65

Table 67: Efficacy of Fungicidal Compositions of Prothioconazole + Cyazofamid + Trifloxystrobin in Potato Crop against Late blight

Table 68: Effect on yield of Fungicidal Compositions of table 67

Table 69: Efficacy of Fungicidal Compositions of Prothioconazole + Cymoxanil + Azoxystrobin in Chilli Crop against Die back

Table 70: Effect on yield of Fungicidal Compositions of table 69

Table 71: Efficacy of Fungicidal Compositions of Prothioconazole + Cymoxanil + Fenaminstrobin in Chilli Crop against Die back

Table 72: Effect on yield of Fungicidal Compositions of table 71

Table 73: Efficacy of Fungicidal Compositions of Prothioconazole + Cymoxanil + Picoxystrobin in Chilli Crop against Die back

Table 74: Effect on yield of Fungicidal Compositions of table 73

Table 75: Efficacy of Fungicidal Compositions of Prothioconazole + Cymoxanil + Pyraclostrobin in Chilli Crop against Die back

Table 76: Effect on yield of Fungicidal Compositions of table 75

Table 77: Efficacy of Fungicidal Compositions of Prothioconazole + Cymoxanil + Pyraoxystrobin in Chilli Crop against Die back

Table 78: Effect on yield of Fungicidal Compositions of table 77

Table 79: Efficacy of Fungicidal Compositions of Prothioconazole + Cymoxanil + Pyraoxystrobin in Chilli Crop against Die back

Table 80: Effect on yield of Fungicidal Compositions of table 79

Table 81: Efficacy of Fungicidal Compositions of Prothioconazole + Dimethomorph + Fenaminstrobin in Chilli Crop against Die back

| T22 | Untreated Control | - | - | - | 44.00 |

Table 82: Effect on yield of Fungicidal Compositions of table 81

Table 83: Efficacy of Fungicidal Compositions of Prothioconazole + Dimethomorph + Pyraoxystrobin in Chilli Crop against Die back

Table 84: Effect on yield of Fungicidal Compositions of table 83

It is evident from the above tables 57-84 that the fungicidal compositions of the present invention gave excellent control on crops against tested fungi-pests wherein all the prepared fungicidal compositions found synergistic as compared to the reference products (solo or binary compositions). It has also been found that use of the compositions of present invention not only gave better efficiency by using low a.i., content compared to binary and solo composition but also broaden the spectrum of compositions as the solo and binary compositions are not providing sufficient fungi-pests control in given duration. Phytotoxicity Observations: Fungicidal compositions of the present invention were found completely safe (non-phy to toxic) on tested crops. In the above field trials, phytotoxicity was assessed for the synergistic composition of the present invention. For phytotoxicity evaluation on Potatoes and Chilli, following observations were made by observing temporary or long lasting damage to the leaves if any viz., leaf injury on tips and leaf surface, wilting, vein clearing, necrosis, epinasty and hyponasty after 10 days of spray of the synergistic fungicidal compositions of the present invention. Crop injury was observed on visual rating from 1-10 scale as given in Table 85. Table 86 provides phytotoxic effect of the synergistic compositions of the present invention on Potatoes and Chilli.

Table 85:

Table 86: Phytotoxic effect of synergistic compositions of the present invention on Potatoes and Chilli crop

It is evident from the above tables 86 that the use of fungicidal compositions of the present invention did not produce any phytotoxic symptoms on the crops. From the foregoing it will be observed that numerous modifications and variations can be effectuated without departing from the true spirit and scope of the novel concepts of the present invention. It is to be understood that no limitations with respect to the specific embodiments illustrated is intended or should be inferred. It should be understood that all such modifications and improvements have been deleted herein for the sake of conciseness and readability but are properly within the scope of the following claims.

Claims

Claim:

1. A synergistic fungicidal compositions comprising: a) first fungicide A, Prothioconazole; b) second fungicide B, selected from Cyazofamid, Cymoxanil or Dimethomorph; c) third fungicide C, selected from Azoxystrobin, Fenaminstrobin, Picoxystrobin, Pyraclostrobin, Pyraoxystrobin or Trifloxystrobin; and d) agriculturally acceptable excipients.

2. The synergistic fungicidal compositions as claimed in claim 1, wherein, a) Prothioconazole is present in an amount in the range from 5 to 30 % w/w; b) Cyazofamid is present in an amount in the range from 0.1 to 10 % w/w or Cymoxanil is present in an amount in the range from 2 to 13 % w/w or Dimethomorph is present in an amount in the range from 15 to 35 % w/w; c) Azoxystrobin is present in an amount in the range from 2 to 15% w/w or Fenaminstrobin is present in an amount in the range from 2 to 15% w/w or Picoxystrobin is present in an amount in the range from 4 to 24% w/w or Pyraclostrobin is present in an amount in the range from 2 to 12% w/w or Pyraoxystrobin is present in an amount in the range from 1 to 12% w/w or Trifloxystrobin is present in an amount in the range from 0.1 to 5% w/w.

3. The synergistic fungicidal compositions as claimed in claim 1, wherein, a) Prothioconazole is present in an amount in the range from 10 to 25 % w/w; b) Cyazofamid is present in an amount in the range from 2 to 7% w/w or Cymoxanil is present in an amount the range from 5 to 10 % w/w or Dimethomorph is present in an amount in the range from 20 to 30 % w/w; c) Azoxystrobin is present in an amount in the range from 5 to 10% w/w or Fenaminstrobin is present in an amount in the range from 5 to 10% w/w or Picoxystrobin is present in an amount in the range from 10 to 18% w/w or Pyraclostrobin is present in an amount in the range from 6 to 8% w/w or Pyraoxystrobin is present in an amount in the range from 4.5 to 8% w/w or Trifloxystrobin is present in an amount in the range from 1 to 3% w/w.

4. The synergistic fungicidal compositions as claimed in claim 1, wherein the composition is one of: a) Prothioconazole is present in an amount in the range from 10 to 25% w/w and Cyazofamid is present in an amount in the range from 2 to 7% w/w and Azoxy strobin is present in an amount in the range from 5 to 10% w/w or Fenaminstrobin is present in an amount in the range from 5 to 10% w/w or Picoxystrobin is present in an amount in the range from 10 to 18% w/w or Pyraclostrobin is present in an amount in the range from 6 to 8% w/w or Pyraoxystrobin is present in an amount in the range from 4.5 to 8% w/w or Trifloxystrobin is present in an amount in the range from 1 to 3% w/w and agriculturally acceptable excipients; b) Prothioconazole is present in an amount in the range from 10 to 25% w/w and Cymoxanil is present in an amount in the range from 5 to 10% w/w and Azoxystrobin is present in an amount in the range from 5 to 10% w/w or Fenaminstrobin is present in an amount in the range from 5 to 10% w/w or Picoxystrobin is present in an amount in the range from 10 to 18% w/w or Pyraclostrobin is present in an amount in the range from 6 to 8% w/w or Pyraoxystrobin is present in an amount in the range from 4.5 to 8% w/w or Trifloxystrobin is present in an amount in the range from 1 to 3% w/w and agriculturally acceptable excipients; c) Prothioconazole is present in an amount in the range from 15 to 25% w/w and Dimethomorph is present in an amount in the range from 20 to 30% w/w and Fenaminstrobin is present in an amount in the range from 7.5 to 10% w/w or Pyraoxystrobin is present in an amount in the range from 5 to 8% w/w and agriculturally acceptable excipients;

5. The synergistic fungicidal compositions as claimed in claim 1, wherein the compositions is one of: a) Prothioconazole is present in an amount in the range from 10 to 20% w/w and Cyazofamid is present in an amount in the range from 2.5 to 6.0% w/w and Azoxystrobin is present in an amount in the range from 6 to 10% w/w and agriculturally acceptable excipients; b) Prothioconazole is present in an amount in the range from 15 to 20% w/w and

Cyazofamid is present in an amount in the range from 2.0 to 6.0% w/w and

Fenaminstrobin is present in an amount in the range from 5 to 9% w/w and agriculturally acceptable excipients; c) Prothioconazole is present in an amount in the range from 12 to 20% w/w and

Cyazofamid is present in an amount in the range from 2.5 to 6.5% w/w and

Picoxystrobin is present in an amount in the range from 10 to 15% w/w and agriculturally acceptable excipients; d) Prothioconazole is present in an amount in the range from 12 to 20% w/w and Cyazofamid is present in an amount in the range from 2 to 6% w/w and Pyraclostrobin is present in an amount in the range from 6 to 8% w/w and agriculturally acceptable excipients; e) Prothioconazole is present in an amount in the range from 15 to 25% w/w and Cyazofamid is present in an amount in the range from 2.5 to 6.5% w/w and Pyraoxystrobin is present in an amount in the range from 4.5 to 8% w/w and agriculturally acceptable excipients; f) Prothioconazole is present in an amount in the range from 15 to 25% w/w and Cyazofamid is present in an amount in the range from 3 to 7% w/w and Trifloxystrobin is present in an amount in the range from 1 to 3% w/w and agriculturally acceptable excipients; g) Prothioconazole is present in an amount in the range from 10 to 20% w/w and Cymoxanil is present in an amount in the range from 5 to 8% w/w and Azoxystrobin is present in an amount in the range from 5 to 10% w/w and agriculturally acceptable excipients; h) Prothioconazole is present in an amount in the range from 15 to 20% w/w and Cymoxanil is present in an amount in the range from 5 to 8% w/w and Fenaminstrobin is present in an amount in the range from 5 to 9% w/w and agriculturally acceptable excipients; i) Prothioconazole is present in an amount in the range from 12 to 20% w/w and Cymoxanil is present in an amount in the range from 6 to 10% w/w and Picoxystrobin is present in an amount in the range from 10 to 18% w/w and agriculturally acceptable excipients; j) Prothioconazole is present in an amount in the range from 12 to 20% w/w and Cymoxanil is present in an amount in the range from 6 to 8% w/w and Pyraclostrobin is present in an amount in the range from 6 to 8% w/w and agriculturally acceptable excipients; k) Prothioconazole is present in an amount in the range from 15 to 25% w/w and Cymoxanil is present in an amount in the range from 5.5 to 9% w/w and Pyraoxystrobin is present in an amount in the range from 5.5 to 8% w/w and agriculturally acceptable excipients; l) Prothioconazole is present in an amount in the range from 12 to 20% w/w and Cymoxanil is present in an amount in the range from 6 to 8% w/w and Trifloxystrobin is present in an amount in the range from 1 to 3% w/w and agriculturally acceptable excipients; m) Prothioconazole is present in an amount in the range from 15 to 20% w/w and

Dimethomorph is present in an amount in the range from 20 to 30% w/w and

Fenaminstrobin is present in an amount in the range from 7.5 to 10% w/w and agriculturally acceptable excipients; n) Prothioconazole is present in an amount in the range from 15 to 25% w/w and

Dimethomorph is present in an amount in the range from 20 to 30% w/w and

Pyraoxystrobin is present in an amount in the range from 5 to 8% w/w and agriculturally acceptable excipients;

6. The synergistic fungicidal compositions as claimed in claim 1, wherein the compositions are formulated as Suspension concentrate (SC), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Flowable concentrate for seed treatment (FS), Oil-dispersion (OD), Water dispersible granules (WDG/WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), preferably Suspension concentrate (SC), and Wettable powder (WP).

7. The synergistic fungicidal compositions, as claimed in claim 1, wherein the agriculturally acceptable excipient is selected from the group comprising dispersing agent, wetting agent, dispersing cum wetting agent, anti-freezing agent, defoamer, biocide, thickener, pH stabilizer, anti-caking agent, diluent or filler.

8. The synergistic fungicidal compositions as claimed in claim 7, wherein, a) the dispersing agent is selected from the group comprising polymethyl methacrylatepolyethylene glycol graft copolymer, acrylate copolymer liquid dispersant, condensed methyl naphthalene sulfonate sodium salt, acrylate copolymer grafted with PEG, polyoxy ethylene alkyl aryl phosphate amine salt, poly-carboxylate dispersant, sodium polycarboxylate, modified styrene acrylic polymeric dispersant, sodium lignosulphonate, sodium salt of naphthalene sulfonate condensate or a combination thereof, and present in an amount in the range from 0.1 to 20% w/w; b) the wetting agent is selected from the group comprising polyoxyethylene alkyl ether, sodium alkyl naphthalene sulfonate blend, Adsee 2610 (non-ionic proprietary blend of supplier), polyalkylene oxide block copolymer, ethoxylated tristyrylphenol, sodium isopropyl naphthalene sulfonate, sodium butyl naphthalene sulfonate, sodium dioctylsulphosuccinate or a combination thereof, and present in an amount in the range from 0.1 to 10% w/w; c) the dispersing cum wetting agent is selected from the group comprising proprietary blend of phosphate ester and sodium naphthalene sulphate formaldehyde condensate, Jeemox SCR (proprietary blend of supplier), modified styrene acrylic polymeric dispersant or a combination thereof, and present in an amount in the range from 2 to 10% w/w; d) the anti-freezing agent is selected from the group comprising glycerol, propylene glycol, diethylene glycol, monoethylene glycol or a combinations thereof, and present in an amount in the range from 1 to 10% w/w; e) the defoamer is selected from the group comprising polydimethylsiloxane antifoam emulsion, silicon emulsion, polysiloxane emulsion or a combination thereof, and present in an amount in the range from 0.01 to 2% w/w; f) the biocide is selected from the group comprising 20% aqueous dipropylene glycol solution of 1, 2-benzisothiazolin-3-one, formaldehyde, isothiazolinone or a combinations thereof, and present in an amount in the range from 0.01 to 1% w/w; g) the thickener is selected from the group comprising xanthan gum, welan gum, guar gum, polyvinyl alcohol, carboxymethylcellulose, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic polymer, starch derivative, polysaccharide, high purity silica, white carbon or a combination thereof, and present in an amount in the range from 0.1 to 0.5% w/w; h) the pH stabilizer is citric acid and present in an amount in the range from 0.1 to 1.0% w/w; i) the anticaking agent is selected from the group comprising precipitated silica, organic bentonite, polyolefin particles, fumed silica or a combination thereof, and present in an amount in the range from 1 to 3% w/w; j) the filler is selected from the group comprising, silica, precipitated silica, sodium bicarbonate, china clay, lactose, bentonite clay, kaolin, diatomaceous earth or a combination thereof, and present in an amount quantity sufficient; and k) the diluents is demineralized water and present in an amount quantity sufficient.