CN102197818A - Bactericidal composition - Google Patents

Abstract

The invention discloses a composition containing bactericidal active substances of SYP-1620 and tricyclazole, and a composition containing bactericidal active substances of SYP-1620 and tricyclazole and potentiator salicylic acid or salicylhydroxamic acid. SYP-1620 and tricyclazole and in a ratio of 1:50-50:1 by weight on the basis of the active components, and the proportion of potentiator salicylic acid or salicylhydroxamic acid to bactericidal active components is 0.1-20% by weight. The composition can be prepared to preparations, such as oil suspension agent, emulsifiable solution, suspending agent, wettable powder and water dispersible granules, and applied in farmlands, orchards or warehouses for controlling plant diseases of cereals, vegetables, fruit trees, Chinese medicinal plants, lawns and tropical crops, such as rice blast, colletotrichum lagenarium, cotton fusarium wilt and the like.

Description

a bactericidal composition

technical field

The invention belongs to the field of agricultural fungicides. The invention relates to a bactericidal composition, in particular to a bactericidal composition containing active components of enoxastrobin and tricyclazole.

Background technique

Enoxastrobin is a new type of fungicide independently developed by Shenyang Research Institute of Chemical Industry. It was first disclosed in Chinese patent application CN1309897A. Its chemical name is: N-methyl-2-[2-((((1-methyl-3-( 2,6-dichlorophenyl)-2-propenyl)amino)oxy)methyl)phenyl]-2-methoxyiminoacetamide, the structural formula is as follows:

Enoxastrobin has a good control effect on various plant diseases on grains, fruit trees, vegetables and other crops, especially on powdery mildew, rust and leaf spot. It has a broad bactericidal spectrum and high activity, has preventive and therapeutic effects, and has good compatibility with environmental organisms. It has a good control effect on various plant diseases caused by fungi such as flagella, zygomycetes, ascomycetes, basidiomycetes and deuteromycetes. Enoxastrobin is a mitochondrial respiration inhibitor, which inhibits the electron transfer of mitochondria, and has a single site of action. The bacteria are prone to adaptive mutations to the drug, which reduces the control effect of the drug, or even becomes ineffective. This type of drug has already become resistant in Europe. related reports. In order to delay the emergence and development of drug resistance of pathogens, and at the same time expand the bactericidal spectrum and provide bactericidal activity, a variety of bactericidal compositions containing enoxastrobin have been developed. However, the development of agricultural production requires the continuous development of new and better products.

Contents of the invention

The object of the present invention is to develop a new fungicidal composition containing enoxistrobin and tricyclazole, which can significantly improve the control effect of crop diseases, and can expand the bactericidal spectrum and delay the development of pathogenic drug resistance. occur. The invention also proposes a technical scheme of adding synergistic substance salicylic acid or salicylic acid into the composition.

Tricyclazole is a broad-spectrum fungicide that has preventive effects against fungal diseases in a variety of crops. It can interact with glyceraldehyde-3-phosphate dehydrogenase in fungal cells, combine with the cysteine-containing protein in the enzyme body, destroy the activity of the enzyme, destroy the metabolism of fungal cells and lose their vitality. The chemical name of tricyclazole is: 5-methyl-[1,2,4]-triazolo[2,4-b]benzothiazole, the structural formula is as follows:

Tricyclazole is an inhibitor of melanin synthesis of plant pathogenic bacteria. Without melanin, pathogens cannot invade plant tissue; tricyclazole is systemic and can quickly transfer in plants, showing high bactericidal activity in actual use. Enoxastrobin belongs to methoxyacrylate fungicides with novel structure and unique mode of action. Since the two types of pesticides have different structure types and different mechanisms of action, the combination of the two has obvious synergistic effect, significantly improves the control effect on crop diseases, and can expand the bactericidal spectrum, and can be used to a greater extent. Delay the emergence and development of pathogenic drug resistance.

Studies have found that adding a small amount of salicylic acid or salicyxamic acid to a fungicidal composition containing erythrastrobin and tricyclazole as active components can significantly improve the bactericidal effect of the composition of erythystrobin and tricyclazole Activity, especially against rice blast and capsicum anthracnose. Salicylic acid is a phenolic substance widely present in plants. It not only participates in many physiological and biochemical processes such as plant flowering, seed germination, stomata opening and closing, heat production, membrane permeability and ion absorption, it is called a new Plant endogenous hormones are also related to plant disease resistance. Salicylic acid has no direct toxicity to pathogens, and can induce rice, tobacco, potato, cucumber, kidney bean and other crops to produce local and systemic resistance to diseases caused by fungi, bacteria, viruses, etc. through exogenous treatment. Some pathogenic fungi have constitutively or inducibly expressed bypass oxidation pathways. When the normal electron transfer pathway is inhibited, electrons can bypass the cyt bcl complex and directly transfer from coenzyme Q to O2, thereby generating a certain amount of energy. Maintain the life activities of fungi. Salicyxamic acid is an inhibitor of bypass oxidase, which can cut off the bypass oxidation pathway of bacteria. It is shown that salicyxamic acid has obvious synergistic effect on some fungicides that interfere with the respiration of bacteria. Therefore, adding a small amount of salicylic acid or salicyxamic acid in the preparation of the composition of enoxastrobin and tricyclazole, or adding a small amount of salicylic acid during use can improve the control effect of the medicament on diseases and significantly reduce the dosage of the medicament. Above-mentioned research result has realized the object of the present invention.

In the fungicidal composition of the present invention, the suitable ratio of parts by weight between the fungicidal active components enoxistrobin and tricyclazole is 1:50 to 50:1; the preferred ratio is 1:20 to 20:1. The appropriate addition amount of the synergistic substance salicylic acid or salicyxamic acid is 0.1-20% by weight of the bactericidal active component; the preferred addition amount is 1-10% by weight of the bactericidal active component. The amount of active ingredient in the composition is from 0.5% to 95% by weight of the total. The amount of active ingredient contained in the product of the composition of the present invention depends on the application rate of the compound when used alone, and also on the ratio of one compound to the other and the degree of synergistic effect, as well as the effect of the synergist. to the role.

The composition of the present invention can be formulated into various dosage forms such as emulsifiable concentrate, oil suspension, suspension concentrate, (water) emulsion, wettable powder, (water dispersible) granule, etc., and can be prepared by common processing methods, for example, active substance and liquid carrier Or after the solid carrier is mixed, add one or several surfactants such as emulsifiers, dispersants, wetting agents, adhesives or defoamers, etc. to prepare through processing. The specific operation process is well known to those skilled in the field of pesticide processing of. The composition contains at least two carriers, at least one of which is a surfactant. The product made of the composition and carrier is diluted or directly mixed with water and sprayed by users before use. The place of application is farmland, orchard, etc. It is used to prevent and control various plant diseases such as rice, grains, vegetables, fruit trees, cotton, Chinese medicinal materials, lawns and tropical crops: such as rice blast, melon anthracnose, pepper anthracnose or cotton wilt illness etc.

The liquid carrier suitable for preparing the bactericidal composition of the present invention includes water, aromatic hydrocarbons, chlorinated aromatic hydrocarbons, aliphatic hydrocarbons, chlorinated aliphatic hydrocarbons, especially polar solvents such as alcohols and their ethers or esters. There are also vegetable oils and methyl lysol. Also, mixtures of different liquids are suitable.

The solid carrier usually used for preparing fungicides can be used as the carrier for preparing the fungicidal composition of the present invention. Such as diatomaceous earth, magnesium aluminum silicate, attapulgite, kaolin, clay, gypsum, bentonite, white carbon black, light calcium carbonate, limestone, hexamethylenediamine, benzofuran resin, styrene polymer or copolymer , corn starch or soluble starch, etc.

The emulsifier suitable for preparing the bactericidal composition of the present invention can be anionic or nonionic emulsifier, such as alkylphenol polyoxyethylene ether, alkylaryl polyoxyethylene polyoxypropylene ether, alkylphenol Polyoxyethylene polyoxypropylene ether, benzylphenol polyoxyethylene ether, polyoxyethylene fatty acid ester, polyoxyethylene fatty alcohol ether, polyoxyethylene fatty amine and other emulsifiers. For example, Nongru 500 ^# , Nongru 600 ^# , Nongru 700 ^# , Nongru OX-635, Nongru OX-662, Nongru OX-690, Nongru OX-2511, Nongru OX-2681, Nongru OX -8686, Agricultural Milk OX-8653C, Agricultural Milk MOA-3, Agricultural Milk OP-10, Agricultural Milk 2201, Agricultural Milk CS-7, Agricultural Milk 507 ^# , Nong Milk 36 ^# , Agricultural Milk 0201B, Agricultural Milk 0203B, Agricultural Milk Milk 0203C, etc.

The dispersant suitable for preparing the fungicidal composition of the present invention can be alkylnaphthalene sulfonate, alkylamido taurate, bis(alkyl)naphthalene sulfonate formaldehyde condensate, naphtholsulfonic acid formaldehyde condensate sodium salt , Alkylphenol polyoxyethylene phosphate, Alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, Alkylphenol polyoxyethylene phosphate, Alkylphenol polyoxyethylene ether, Castor oil ethylene oxide addition substances, ethylene oxide-propylene oxide block copolymer, alkylphenol polyoxyethylene ether formaldehyde condensate, alkylphenol polyoxyethylene polyoxypropylene ether, methyl cellulose, etc. For example, sodium lignosulfonate, calcium lignosulfonate, alkylnaphthalenesulfonate formaldehyde condensate, sodium methylnaphthalenesulfonate formaldehyde condensate, naphthalenesulfonate formaldehyde condensate, sodium methylenenaphthalenesulfonate, oleic acid formaldehyde Sodium amino ethyl sulfonate, alkyl-diethylene glycol ether-sodium sulfonate, N-methyl-oleoyl-sodium taurate, epoxy polyether, p-tert-butyl ether, dibutyl naphthalene sulfonate Acid formaldehyde condensates and sodium hexametaphosphate, etc.

The wetting agent suitable for preparing the fungicidal composition of the present invention can be sodium lauryl sulfate, sodium lauryl sulfate, sodium dodecylsulfonate, sodium alkylbenzenesulfonate, sodium alkylnaphthalenesulfonate, sorrel Alcohol fatty acid ester polyoxyethylene ether, etc.

Adhesives suitable for the preparation of the fungicidal composition of the present invention may be natural or synthetic, such as xanthan gum, gelatin, gum arabic, polyvinylpyrrolidone, magnesium aluminum silicate, polyvinyl alcohol, phenolic resins, shellac, carboxyl Methylcellulose, methylcellulose and sodium alginate, etc. The defoaming agent can be foam enemy, silicone, C _{8 ~ 10} fatty alcohol, C _{10 ~ 20} saturated fatty acid (such as capric acid) and amides, etc.

The products described in the present invention can also be provided in the form of finished formulations, ie the substances of the composition have been mixed. At the same time, the active ingredients of the bactericidal composition can also be provided in the form of separate formulations, which are directly mixed in barrels (tanks) by users before use. The concentrates of the present invention are generally mixed with water to obtain the desired concentration of active substance.

The fungicidal composition of the present invention can also be used in combination with other compounds having insecticidal or fungicidal activity, and can also be used in combination with nematicides, protective agents, growth regulators, plant nutrients or soil regulators. The composition of the present invention can be applied by common methods, such as seed soaking, seed dressing, pouring, spraying, spreading and the like. The application amount of the present invention varies with weather conditions or crop status, and the application period can be before or after crops are susceptible to disease. The duration of the protective effect is usually related to the content of the individual compounds in the composition, but also to external factors, such as climate, which can be mitigated by using appropriate formulations.

The bactericidal composition of the present invention has the following advantages: one, the composition shows excellent synergistic effect within a certain proportion range, and the mixed composition has significantly improved bactericidal effect compared with its single agent, thereby reducing the dosage, While reducing the cost of pesticides for farmers, the degree of impact on the environment is reduced; especially after adding a small amount of salicylic acid to the mixture, the bactericidal activity of the agent is greatly improved. The second is that the application of the composition can delay the occurrence and development of resistance to a single agent, the structure of the varieties is quite different, and there is no cross-resistance; Mixed with the fungicide fenoxastrobin, it has positive significance for disease control and resistance management.

Detailed ways

The following specific examples are used to illustrate the present invention in detail, but the present invention is by no means limited to these examples. The percentages in the formulations of all preparations are percentages by weight, and the treatment doses of biologically active parts are all active ingredient contents.

recipe example

Preparation of emulsifiable concentrate: According to the formula requirement, add solvent, original drug and emulsifier respectively, mix evenly, heat and dissolve with hot water bath if necessary, and obtain transparent emulsifiable concentrate. As example 1, example 2 formula.

Example 110% emulsifiable concentrate

Enoxastrobin 8%, tricyclazole 2%, Nongru OX-2681 9.5%, Nongru OX-8686 5.5, dimethylformamide 10%, and xylene supplemented to 100%.

Example 2 15% EC

Enoxastrobin 5%, Tricyclazole 10%, Salicylic Acid 0.2%, Nongru OX-2681 6.5%, Nongru OX-8686 3.0, Nongru 600#2.0%, Dimethylformamide 8%, Dimethylformamide Toluene made up to 100%.

Preparation of wettable powder: According to the formula requirements, the original drug, various additives and carriers are fully mixed, and after being pulverized by an ultra-fine pulverizer, the processed product is obtained. As example 3, example 4 formula.

Example 3 20% wettable powder

Enoxastrobin 4%, Tricyclazole 16%, Salicyxamic Acid 1%, Sodium Lauryl Sulfate 1.5%, Sodium Naphthalene Sulfonate Formaldehyde Condensate 4%, Sodium Lignosulfonate 4%, White Carbon Black 3%, light calcium carbonate topped up to 100%.

Example 4 50% wettable powder

Enoxastrobin 40%, Tricyclazole 10%, Sodium Lauryl Sulfate 2.0%, Sodium Salt of Alkyl Naphthalene Sulfonic Acid Condensation Polymer 2%, Sodium Lignosulfonate 5%, Detergent LS 2%, White carbon black 5%, light calcium carbonate make up to 100%.

Preparation of water dispersible granules: According to the formula requirements, after mixing the pesticide technical, dispersant, wetting agent and carrier evenly, after pulverizing by a jet mill, add a certain amount of aqueous solution containing a binder, kneading, Then add it to a granulator equipped with a certain size screen for extrusion and granulation, and then dry and sieve (according to the range of the screen) to obtain a granular product. As example 5, example 6 formula.

Example 5 30% water dispersible granules

Enoxastrobin 15%, tricyclazole 15%, sodium methylene naphthalene sulfonate 5%, epoxy polyether 5%, soluble starch 10%, sodium sulfate 15%, gypsum supplemented to 100%.

Example 6 50% water dispersible granules

Enoxastrobin 10%, tricyclazole 40%, salicylic acid 1%, sodium salt of alkylnaphthalene sulfonic acid condensation polymer 5%, sodium alkylaryl sulfonate mixture 2%, ammonium sulfate 10%, formazan Base cellulose 3%, gypsum make up to 100%.

Preparation of suspension

According to the formula requirements, using water as the medium, mix the original drug, dispersant, suspending agent and antifreeze, put it into the sand mill, and grind it finely. The particle size distribution of the solid in the feed liquid is detected by a particle size distribution instrument. After passing the test, the material is discharged to obtain a suspending agent. As example 7, example 8 formula.

Example 7 20% suspending agent

Enoxastrobin 5%, Tricyclazole 15%, Nongru 0201B 2.5%, Sodium Naphthalene Sulfonate Formaldehyde Condensate 2.5%, Calcium Lignosulfonate 2.5%, White Carbon Black 0.1%, Ethylene Glycol 4%, Disinfectant Add a little foaming agent and make up to 100% with water.

Example 8 45% suspension concentrate

Enoxastrobin 5%, Tricyclazole 40%, Salicylic Acid 0.8%, Sodium Naphthalene Sulfonate Formaldehyde Condensate 2%, Sodium Salt of Alkyl Naphthalene Sulfonate Condensate 2%, Polyoxyethylene Sorbitan Monolaurate Ester 0.5%, ethylene glycol 4.5%, a little defoamer, water to make up to 100%.

Preparation of oil suspension

According to the formula requirements, using methylated soybean oil as the medium, mix the original drug and dispersant, put it into a sand mill, and grind it finely. The particle size distribution of the solid in the feed liquid is detected by a particle size distribution instrument. After passing the test, After discharging, the oil floating agent can be obtained. As example 9, example 10 formula.

Example 9 14% oil suspension

Enoxastrobin 4%, Tricyclazole 10%, Salicylic Acid 0.3%, Sorbitan Monooleate 1%, Agricultural Milk NP-7-Phosphate 0.5%, Agricultural Milk 0201B 2.0%, Agricultural Milk OX-65 61.5%, Methylated soybean oil complemented to 100%.

Example 10 20% oil suspension

Enoxastrobin 5%, Tricyclazole 15%, Salicylic Acid 0.5%, Sorbitan Monooleate 1.5%, Nongru NP-7-Phosphate 0.5%, Nongru 0201B 3.0%, C ₁₂ Fat Alcohol polyoxyethylene (3) ether 1.0%, methylated soybean oil supplemented to 100%.

Examples of Indoor Bioactivity

The synergistic effect of example 11 oxastrobin and tricyclazole

There are usually three results after mixing two or more drugs, namely: synergism, addition and antagonism. Synergy is the expected result.

Mix enoxastrobin and tricyclazole according to the weight ratio of 16:1, 8:1, 4:1, 2:1, 1:1, 1:2, 1:4, 1:8, 1:16 After mixing, set 5 concentrations and use a crop sprayer to spray rice seedlings with 2 leaves and 1 heart, and place them in a ventilated hood to dry. After 24 hours, the rice blast (Pyricularia grisea Sacc) spore suspension was inoculated, and then moved to an artificial climate chamber for moisture cultivation. After 6 days, the control effect was investigated, and the control effect was calculated by the disease index, and the toxicity regression equation and EC ₉₀ were obtained. According to the Wadley fungicide mixture combined effect evaluation method, the interaction ratio of the two single agents was calculated.

Wadley mixture joint action evaluation method:

The interaction level can be expressed by the interaction ratio R, which is calculated as follows:

R=EC ₉₀ (theoretical value)/EC ₉₀ (observed value)

Evaluation: When 0.5<R<1.5, the two compounds have an additive effect after mixing; when R≥1.5, it means that there is a synergistic effect; when R≤0.5, it means that there is an antagonistic effect.

The theoretical reaction EC ₉₀ (theoretical value) to the mixture containing two components can be calculated according to the following equation:

A and B represent two components respectively, and a and b are the ratios of the contents of each component in the mixture.

EC ₉₀ (A) is the actual observed response data of a single agent of A component (that is, the effective concentration when producing 90% preventive effect); EC ₉₀ (B) is the actual observed response data of a single agent of B component ( Effective concentration when producing 90% preventive effect); EC ₉₀ (observed value) is the actually observed reaction data after component A+B (effective concentration when producing 90% preventive effect).

The inventor has found through a large number of screening tests that after the mixing of enoxastrobin and tricyclazole (4: 1, 2: 1, 1: 1, 1: 2, 1: 4 , 1:8, 1:16) ratio, there is an extremely significant synergistic effect (see Table 1), not just the simple additive effect of the two agents.

Table 1 Synergistic effect result of erythoxystrobin and tricyclazole (rice blast)

The synergistic effect of example 12 salicylic acid and salicyxamic acid

Rice blast: mix enoxastrobin and tricyclazole according to the weight ratio of 1:2 and 1:3, and then make it into a formula containing 0.5mg/L salicylic acid, 1mg/L salicylic acid and no salicylic acid The concentration of the mixture of enoxastrobin and tricyclazole was 2 mg/L liquid, and a single-dose control of the same treatment and a blank control without spraying were set up. Paddy rice seedlings with 2 leaves and 1 heart are selected to be sprayed on a crop sprayer, and placed in a ventilated hood after spraying to dry. After 24 hours, the rice blast (Pyricularia grisea Sacc) spore suspension was inoculated, and then moved to an artificial climate chamber for moisture cultivation. After 6 days, the control effect was investigated, and the control effect was calculated by the disease index.

Cucumber anthracnose: mix erythrastrobin and tricyclazole according to the weight ratio of 1:1 and 4:1, and then make it into a mixture containing 1mg/L salicyxamic acid, 2mg/L salicyxamic acid and not The concentration of the mixture of enoxastrobin and tricyclazole containing salicylic acid was 4 mg/L liquid solution, and a single agent control of the same treatment and a blank control without spraying were set up. Select the cucumber seedlings of 2 true leaves to carry out spraying treatment on the crop sprayer, and place the ventilation hood after spraying to dry. After 24 hours, the spore suspension of Colletrichum lagenarium (Pass.) Ell. et. Halstt was inoculated, and then moved to an artificial climate chamber for moisture cultivation. After 6 days, the control effect was investigated, and the control effect was calculated by the disease index.

Adding a small amount of salicylic acid or salicyxamic acid to the composition of enoxastrobin and tricyclazole can significantly improve the activity of the composition of enoxastrobin and tricyclazole. The application examples in Table 2 fully illustrate up to this point.

Table 2 Synergistic effect of salicylic acid or salicyxamic acid

Examples of control effects in field plots

The experiment was carried out under natural conditions in the field, and the experimental crops were cultivated and managed according to the conventional requirements of agricultural production. In the field plot test, 3 concentrations of the tested chemicals were set, and a blank control without the drug was set up. The method of application is leaf spraying, and the application equipment is knapsack-type manual sprayer. All the trials in plots are natural disease, and the result investigation is carried out when the blank control without pesticides is fully diseased. The disease classification and control effect calculation methods are all in accordance with the national standard of the People's Republic of China "Guidelines for Field Efficacy Tests of Pesticides".

(1) Investigation method and grading standard of rice blast (panicle blast):

The five-point sampling method was used to sample, 50 ears were investigated at each point, and a total of 250 ears were investigated in each plot, and the total number of surveyed leaves and the number of diseased leaves at all levels were recorded.

Grading standards are as follows:

Grade 0: no disease; Grade 1: loss of less than 5% per ear (individual branch stalk disease); 3 grade: loss of 6-20% per ear (about one-third of branch stem disease); 5 grade: loss of 21 per ear -50% (ear stalk or spindle disease, grain half-shrunk); 7th grade: loss of 51-70% per ear (ear stalk disease, most shriveled grain); 9 grade: loss of more than 70% per ear (ear stalk disease , causing white spikes).

(2) Investigation method and grading standard of chili anthracnose:

The five-point sampling method was adopted for sampling, and 50 fruits were surveyed at each point, and graded according to the percentage of lesion area in the whole fruit area.

Grading standards are as follows:

Level 0: no disease class; level 1: lesion area accounts for less than 2% of the fruit area; level 3: lesion area accounts for 3-8% of the fruit area; level 5: lesion area accounts for 9-15% of the fruit area; level 7 : The lesion area accounts for 16-25% of the fruit area; Grade 9: The lesion area accounts for more than 25% of the fruit area.

(3) Calculation method of efficacy

The synergistic effect of example 13 control rice blast

The processing preparation (that is, formulation example 1, 2, 4, 5, 7, 8, 9, 10) mixed with tricyclazole and tricyclazole is mixed according to the concentration of 200mg/L, 100mg/L and 50mg/L respectively Water, spray once at the peak tillering stage of rice and at the end of booting stage of rice. A single dose control of 75% tricyclazole wettable powder and 5% oxastrobin emulsifiable concentrate of the same concentration, and a blank control sprayed with water were set.

The test results show that the mixture of fenoxastrobin and tricyclazole also shows a good synergistic effect in the field, and the drug effect after mixing is significantly higher than that of a single agent, and it has a very good control effect on rice blast; it contains salicylic acid Acid, enoxastrobin and tricyclazole mixture (embodiments 2, 8, 10) have a more prominent control effect on rice blast, and a higher control effect can be obtained with less dosage. The results are shown in Table 3.

Table 3 embodiment is to the control effect of rice blast

*: The new multiple range method is used for significance analysis, significance at the 5% level; a, b, c, and d with the same letter mark indicate no significant difference among treatments, and different letter marks indicate significant difference among treatments sexual difference.

The effect of example 14 on increasing the yield of rice

Mix formula examples 8, 9, and 10 with water at concentrations of 200 mg/L, 100 mg/L, and 50 mg/L, respectively, and spray once at the peak tillering stage of rice and at the end of the booting stage of rice. A single dose control of 75% tricyclazole wettable powder and 5% oxastrobin emulsifiable concentrate of the same concentration, and a blank control sprayed with water were set. After the rice matured, each plot was single harvested and harvested to measure the yield. Record the yield of plots, and apply the new multiple range method to analyze the significance of the yields of different treatments. At the same time, calculate the average yield of the 3 plot yields.

The results show that: the mixture of erythystrobin and tricyclazole shows a good yield-increasing effect in the field, and the yield of paddy rice in plots treated with the medicament is obviously improved, and the yield increase rate of rice in Example 10 exceeds 15% under the treatment concentration of 200mg/L. %. The results are shown in Table 4.

The embodiment of table 4 affects rice yield

blank control Shimizu / 27.83 / 515.41h

*: The area of the plot is 36 square meters, and the output is the average of the output of the 3 plots.

**: The new multiple range method is used for significance analysis, significance at the 5% level; when a, b, c, d, e, f, g, and h are marked with the same letter, it means that there is no significant difference between treatments, and there is no significant difference between treatments. The same letters indicate significant differences among treatments.

Example 15 Control effect of oxastrobin and tricyclazole mixed on pepper anthracnose

Mix formula examples 3, 4, and 6 with water at concentrations of 200 mg/L, 100 mg/L, and 50 mg/L respectively, and start spraying at the young pepper stage, and spray 3 times continuously. A single dose control of 75% tricyclazole wettable powder and 5% oxastrobin emulsifiable concentrate of the same concentration, and a blank control sprayed with water were set. 8 days after the last spraying, the control effect was investigated.

The result shows: the effect of salicyxamic acid on significantly improving the effect of enoxastrobin, under the same treatment dosage, the mixed compound containing enoxastrobin, tricyclazole and salicyxamic acid has the best control effect in the field (implementation Example 3, 6). The results are shown in Table 5.

The control effect of table 5 embodiment to capsicum anthracnose

*: The new multiple range method was used for significance analysis, significance at the 5% level; a, b, and c marked with the same letter indicate no significant difference among treatments, and different letter marks indicate significant difference among treatments .

Claims (7)

1. bactericidal composition is characterized in that: contain two kinds of active components of alkene oxime amine and tricyclazole, the ratio of weight and number between two kinds of active components is 1: 50 to 50: 1; Active component weight percentage in the composition is 0.5-95%.

2. bactericidal composition according to claim 1 is characterized in that: the ratio of weight and number between two kinds of active components of alkene oxime amine and tricyclazole is 1: 20 to 20: 1.

3. bactericidal composition according to claim 2 is characterized in that: the ratio of weight and number between two kinds of active components of alkene oxime amine and tricyclazole is 1: 16 to 4: 1.

4. bactericidal composition according to claim 1 is characterized in that: also contain synergistic matter salicylic acid or the acid of bigcatkin willow oxime in the composition, addition is the 0.1-20% of bactericidal active constituent weight.

5. bactericidal composition according to claim 4 is characterized in that: the synergistic matter addition is the 1-10% of bactericidal active constituent weight.

6. according to claim 1 or 4 described bactericidal compositions, it is characterized in that: said composition can be mixed with missible oil, oil suspending agent, suspending agent, emulsion, aqueous emulsion, wetting powder, granula or water dispersible granules.

7. the purposes of bactericidal composition according to claim 6 is characterized in that: be used to prevent and treat rice blast, melon anthracnose, pepper anthracnose or cotton wilt.