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WO2023113354A1 - Kit de composition d'encre biologique pour tissu biologique tubulaire, son procédé de fabrication, procédé de construction de tissu biologique tubulaire l'utilisant, et tissu biologique tubulaire construit par ledit procédé - Google Patents

Kit de composition d'encre biologique pour tissu biologique tubulaire, son procédé de fabrication, procédé de construction de tissu biologique tubulaire l'utilisant, et tissu biologique tubulaire construit par ledit procédé Download PDF

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Publication number
WO2023113354A1
WO2023113354A1 PCT/KR2022/019736 KR2022019736W WO2023113354A1 WO 2023113354 A1 WO2023113354 A1 WO 2023113354A1 KR 2022019736 W KR2022019736 W KR 2022019736W WO 2023113354 A1 WO2023113354 A1 WO 2023113354A1
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WIPO (PCT)
Prior art keywords
tubular
hydrogel
sacrificial
biological tissue
forming
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2022/019736
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English (en)
Korean (ko)
Inventor
김민균
김서연
남상균
이희경
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Industry Foundation of Chonnam National University
Original Assignee
Industry Foundation of Chonnam National University
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Publication date
Priority claimed from KR1020220163090A external-priority patent/KR20230089541A/ko
Application filed by Industry Foundation of Chonnam National University filed Critical Industry Foundation of Chonnam National University
Publication of WO2023113354A1 publication Critical patent/WO2023113354A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/04—Hollow or tubular parts of organs, e.g. bladders, tracheae, bronchi or bile ducts
    • A61F2/06—Blood vessels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/52—Hydrogels or hydrocolloids

Definitions

  • the present invention relates to a tubular biological tissue such as a blood vessel or a nerve conduit and a method for manufacturing the same, and more specifically, to form a tubular biological tissue, two solutions having different physical properties by having different components are formed, i.e., a core portion, which is an inner core.
  • a bioink composition kit composed of a sacrificial solution to form a tubular structure surrounding the core and a hydrogel forming a tube, and use the bioink composition kit to print two heads provided in a 3D printer under different process conditions.
  • It relates to a bioink composition kit for tubular biological tissues that can be easily produced by simultaneous operation to produce tubular biological tissues having various shapes, a method for manufacturing the same, a method for manufacturing tubular biological tissues using the same, and a tubular biological tissue produced by the method .
  • Tubular biological tissues have different diameters, lengths, shapes, curvatures, changes in diameters, and constituent cells depending on the organ site, and there are various types such as tubular structures composed of multi-layered tissue structures such as 2 and 3 layers as well as single-layer structures. .
  • the conventional coaxial nozzle-based tubular biological tissue manufacturing method has poor self-standing ability due to weak mechanical properties of the hydrogel after printing and curing. Such a decrease in shape-retaining ability causes difficulty in maintaining a circular tubular cross-sectional shape, yield reduction when manufacturing a curved pipe shape, and difficulty in connecting a perfusate for culturing tubular biological tissue.
  • the present inventors have completed the present invention by developing a tubular biological tissue manufacturing technology with improved mechanical properties as a result of numerous studies.
  • an object of the present invention is to provide bioink in which two solutions having different physical properties due to different components, that is, a sacrificial solution for forming a core portion, which is the inner center, and a tube-forming hydrogel forming a tubular structure surrounding the core portion are separated and packaged. It is to provide a composition kit and a manufacturing method thereof.
  • Another object of the present invention is to simultaneously drive two heads provided in a 3D printer under different process conditions using a bioink composition kit to easily form tubular biological tissues having various shapes with a 3D printer, thereby enabling various bends. It is to provide a method for manufacturing tubular biological tissue using 3D printing, which can increase the yield when manufacturing the shape.
  • Another object of the present invention is to improve self-standing ability by increasing mechanical properties, so that it is easy to maintain a circular tubular cross-sectional shape after printing and curing, and it is easy to connect perfusate for culturing tubular biological tissue. It is to provide a tubular biological tissue.
  • the present invention is LithiumPhenyl (2,4,6-trimethylbenzoyl) phosphinate (LAP) 0.03 to 0.07% by weight, Gelatin methacrylate (GelMA) 5 to 15% by weight and alginate 2 to Conduit-forming hydrogel containing 3% by weight; and a sacrificial hydrogel containing 0.05 to 0.15N calcium chloride and 30 to 50% by weight of pluronic F-127.
  • LAP LithiumPhenyl (2,4,6-trimethylbenzoyl) phosphinate
  • Gelatin methacrylate Gelatin methacrylate
  • alginate 2 to Conduit-forming hydrogel containing 3% by weight
  • a sacrificial hydrogel containing 0.05 to 0.15N calcium chloride and 30 to 50% by weight of pluronic F-127.
  • the conduit-forming hydrogel contains phosphate buffered saline (PBS).
  • PBS phosphate buffered saline
  • conduit-forming hydrogel and the sacrificial-forming hydrogel are separately packaged and stored in a refrigerator.
  • the present invention is a conduit-forming hydrogel preparation step; And a sacrificial formation hydrogel preparation step; provides a method for manufacturing a bioink composition kit for tubular biological tissues independently performed.
  • the conduit-forming hydrogel preparation step is to prepare a 0.05 to 0.15% by weight LAP solution by dissolving LithiumPhenyl (2,4,6-trimethyl benzoyl) phosphinate (LAP) in PBS; Preparing 15 to 25% by weight of GelMA hydrogel by dissolving Gelatin methacrylate (GelMA) in the LAP solution; Preparing an alginate hydrogel of 4 to 5.5% by weight by dissolving alginate in distilled water; and preparing a conduit-forming hydrogel by mixing the GelMA hydrogel and the alginate hydrogel at a volume ratio of 1:1.
  • LAP LithiumPhenyl (2,4,6-trimethyl benzoyl) phosphinate
  • the dissolution is performed by selectively repeating one or more of vortexing, storage in a water bath at 37 ⁇ 0.5 ° C, and water bath at 180 ⁇ 0.5 ° C.
  • the mixing of the GelMA hydrogel and the alginate hydrogel is performed by repeatedly performing 37 ⁇ 0.5 ° C water bath storage and vortexing.
  • the step of preparing the sacrificial hydrogel may include preparing a 0.05 to 0.15N calcium chloride solution by adding calcium chloride to distilled water; and adding 30 to 50% by weight of pluronic F-127 to the 0.05 to 0.15N calcium chloride solution and mixing.
  • the mixing step is performed by repeatedly performing low-temperature storage and vortexing.
  • the present invention is a 3D printer of the duct-forming hydrogel and the sacrificial hydrogel of any one of the above-described bioink composition kits for tubular biological tissues or the bioink composition kit for tubular biological tissues prepared by any one of the above-described manufacturing methods.
  • loading into A pipeline structure formed by simultaneously driving the two heads provided in the 3D printer under different process conditions to form a sacrificial body, which is a core portion, with the sacrificial hydrogel, and forming a tubular structure surrounding the core portion with the conduit-forming hydrogel. outputting; Curing the tubular structure by UV irradiating the surface of the pipeline structure; and cutting both ends of the cured pipeline structure and then removing the sacrificial material to form a hollow core portion.
  • the process conditions of the head for outputting the tubular structure with the tube-forming hydrogel in the outputting step are 26 ⁇ 0.5° C. and 220 to 230 kPa, and the head for outputting the sacrificial material with the sacrificial hydrogel.
  • the process conditions are 35 ⁇ 0.5°C and 120 ⁇ 130kPa.
  • the UV irradiation is performed for 150 seconds to 210 seconds so that the amount of light reaching the surface of the pipeline structure is 65 to 75 mW/cm 2 .
  • the step of removing the sacrificial material comprises dissolving the sacrificial material by injecting PBS or distilled water into one end of the cut pipeline structure; and inhaling the liquid present in the hollow of the pipeline structure.
  • the size of the hollow in the pipeline structure and the thickness of the tubular structure are determined according to the diameter of the nozzle used in the 3D printing.
  • the present invention provides a tubular biological tissue produced by the above-described manufacturing method.
  • bioink composition kit of the present invention two solutions having different physical properties due to different components, that is, a sacrificial solution for forming the core part, which is the inner center, and a tube-forming hydrogel for forming a tubular structure surrounding the core part are separated and packaged. Therefore, it maintains its physical properties well and is convenient to use.
  • the method for producing tubular biological tissue using 3D printing of the present invention simultaneously drives two heads provided in a 3D printer under different process conditions using a bioink composition kit to 3D print tubular biological tissues having various shapes. It can be easily formed, so the yield can be increased when manufacturing various bends.
  • the mechanical properties are increased and the self-standing ability is excellent, so it is easy to maintain the circular tubular cross-sectional shape after printing and curing, and the perfusate for culturing the tubular biological tissue Easy to connect.
  • FIG. 1a to 1d are photographs showing one embodiment of the step of preparing a LAP solution performed during the preparation step of the hydrogel to form a conduit in the method for manufacturing a bioink composition kit according to various embodiments of the present invention.
  • FIGS. 2a to 2d are photographs showing one embodiment of a step of preparing a GelMA solution.
  • 3a to 3c are photographs showing one embodiment of the step of preparing an alginate solution.
  • FIGS. 4a to 4c are photographs showing one embodiment of a step of preparing a tubular hydrogel.
  • FIG. 5 is a photograph showing one embodiment of the sacrificial material formation hydrogel preparation step in the bioink composition kit manufacturing method according to various embodiments of the present invention.
  • Figure 6a is a photograph showing that the conduit-forming hydrogel prepared according to Figs. 4a to 4c is cured by UV irradiation
  • Fig. 6b is a photograph showing that it is not cured without UV irradiation.
  • FIG. 7 is a schematic diagram showing the implementation principle of a method for manufacturing tubular biological tissue using 3D printing according to various embodiments of the present invention.
  • Figure 8a is a photograph showing the durability of the output produced by the manufacturing method of the present invention can be picked up with tweezers
  • Figs. 8b to 8d are photographs showing various types of tubular biological tissues manufactured by the manufacturing method of the present invention.
  • 9a and 9b are photographs of the result of evaluating the perfusion performance of the straight conduit manufactured by the manufacturing method of the present invention.
  • 10a to 10c are photographs of the results of evaluating the perfusion performance of the U-shaped conduit manufactured by the manufacturing method of the present invention.
  • first and second may be used to describe various components, but the components should not be limited by the terms. These terms are only used for the purpose of distinguishing one component from another. For example, a first element may be termed a second element, and similarly, a second element may be termed a first element, without departing from the scope of the present invention.
  • temporal precedence relationship for example, when a temporal precedence relationship is described as 'after', 'continue to', 'after ⁇ ', 'before', etc., 'immediately' or 'directly' Including non-consecutive cases unless ' is used.
  • the technical feature of the present invention is a bioink composition kit that maintains physical properties and is convenient to use because the sacrificial solution forming the core part, which is the inner center, and the tube-forming hydrogel forming the tubular structure surrounding the core part are separated and packaged.
  • Tubular biological tissues having various shapes can be easily formed with a 3D printer by simultaneously driving the two heads provided in the 3D printer under different process conditions, thereby increasing the yield when producing various bends using a 3D printer.
  • the present invention is a bioink composition of a new composition that can compensate for the disadvantages of the existing coaxial nozzle-based tubular biological tissue manufacturing method, which has poor self-standing ability due to weak output and mechanical properties of the hydrogel after curing. Because the kit was developed.
  • the bioink composition kit for tubular living tissue of the present invention contains 0.03 to 0.07% by weight of LithiumPhenyl (2,4,6-trimethylbenzoyl)phosphinate (LAP), 5 to 15% by weight of Gelatin methacrylate (GelMA), and 2 to 3% by weight of alginate.
  • Conduit-forming hydrogel containing % and the remaining amount of solvent; and a sacrificial hydrogel containing a 0.05 to 0.15N calcium chloride solution and a 30 to 50 wt % pluronic F-127 aqueous solution.
  • alginate or the like included in the conduit-forming hydrogel enhances the viscosity before curing.
  • alginate can be cured to create a small-sized egg-box structure by adding ions, an interpenetrated network (IPN) in which the two materials cross each other during the subsequent photocuring reaction of GelMA, a photoreactive biomaterial By forming, it is possible to further increase the mechanical strength.
  • IPN interpenetrated network
  • the duct-forming hydrogel may contain phosphate buffered saline (PBS), which may be an aqueous salt solution containing disodium hydrogen phosphate and sodium chloride of known composition, or an aqueous salt solution containing potassium chloride and potassium dihydrogen phosphate. there is.
  • PBS phosphate buffered saline
  • the sacrificial hydrogel can be printed with a 3D printer and must have properties that can be dissolved in an aqueous solvent for a short time in the printed state, it is implemented to include 0.05 to 0.15N calcium chloride solution and 30 to 50% by weight of pluronic F-127. did In particular, calcium chloride (CaCl 2 ) contained in the sacrificial hydrogel can immediately cause ionic curing of alginate contained in the conduit-forming hydrogel.
  • conduit-forming hydrogel and the sacrificial-forming hydrogel may be separately packaged and refrigerated.
  • the method for manufacturing a bioink composition kit for tubular biological tissues of the present invention includes the preparation step of a hydrogel forming a conduit; and sacrificial solution preparation step; performed independently.
  • the conduit-forming hydrogel preparation step is to prepare a 0.05 to 0.15% by weight LAP solution by dissolving LithiumPhenyl (2,4,6-trimethyl benzoyl) phosphinate (LAP) in PBS; Preparing 15 to 25% by weight of GelMA hydrogel by dissolving Gelatin methacrylate (GelMA) in the LAP solution; Preparing an alginate hydrogel of 4 to 5.5% by weight by dissolving alginate in distilled water; and preparing a conduit-forming hydrogel by mixing the GelMA hydrogel and the alginate hydrogel at a volume ratio of 1:1.
  • LAP LithiumPhenyl (2,4,6-trimethyl benzoyl) phosphinate
  • Dissolution performed in each step can be achieved by selectively repeating one or more of vortexing, storage in a water bath at 37 ⁇ 0.5 ° C, and hot water at 180 ⁇ 0.5 ° C. That is, as shown in Figures 1a to 3c, as one embodiment, the step of preparing the LAP solution is performed only vortexing, and the step of preparing the GelMA hydrogel is vortexing and 37 ⁇ 0.5 °C water bath storage alternately GelMA It can be repeated until it is completely dissolved, and the step of preparing the alginate hydrogel can be performed with water bath at 180 ⁇ 0.5 ° C. after vortexing.
  • the mixing of the GelMA hydrogel and the alginate hydrogel can be performed by repeatedly performing 37 ⁇ 0.5 ° C water bath storage and vortexing, as shown in FIGS. 4a to 4c.
  • the sacrificial material formation hydrogel preparation step is to prepare a 0.05 to 0.15N calcium chloride solution by adding calcium chloride to distilled water; and adding 30 to 50% by weight of pluronic F-127 to the 0.05 to 0.15N calcium chloride solution and mixing.
  • the mixing step may be performed by repeatedly performing low temperature storage and vortexing as shown in FIG. 5 .
  • the method for producing tubular biological tissues using 3D printing of the present invention is a conduit of any one of the above-described bioink composition kits for tubular biological tissues or a bioink composition kit for tubular biological tissues manufactured by any one of the above-described manufacturing methods.
  • the tube-forming hydrogel and the sacrificial-forming hydrogel are injected into two syringes, and a coaxial nozzle is inserted into the syringe into which the sacrificial-forming hydrogel is placed. It can be performed by attaching a plastic nozzle to a syringe into which hydrogel is injected and then attaching each to a 3D printer head.
  • the output step is a step of outputting a pipeline structure composed of a core portion and a tubular structure through a coaxial nozzle connected by simultaneously driving two heads provided in the 3D printer, which is a process of a head that outputs a tubular structure with a conduit-forming hydrogel Conditions are 26 ⁇ 0.5 °C, 220 ⁇ 230 kPa, process conditions of the head outputting the sacrificial material to the sacrificial hydrogel may be 35 ⁇ 0.5 °C, 120 ⁇ 130 kPa.
  • UV irradiation may be performed for 150 seconds to 210 seconds so that the amount of light reaching the surface of the pipeline structure is 65 to 75 mW/cm 2 .
  • the step of removing the sacrificial object may include dissolving the sacrificial object by injecting PBS or distilled water into one end of the cut pipeline structure; and inhaling the liquid present in the hollow of the pipeline structure.
  • FIG. 3 The implementation principle of the method for manufacturing a tubular biological tissue using 3D printing according to various embodiments of the present invention having the above configuration is shown in FIG. 3 .
  • FIG. 7 it is possible to print a long cylindrical tube with different inner and outer materials by simultaneously using the two heads provided in the 3D printer at different air pressures and different temperatures.
  • alginate and Ca ions meet Using the property of curing, it can be seen that the inner wall of the conduit is cured by CPF-127 (Pluronic F127/CaCl2) and the outer wall of the conduit is cured by UV.
  • the tubular biological tissue of the present invention is manufactured by the above-described manufacturing method, as can be seen through the experimental examples described later, the mechanical properties are increased and the self-standing ability is excellent, so that after printing and curing, it has a circular shape. It is easy to maintain the tubular cross-sectional shape, and it is easy to connect the perfusate for culturing the tubular biological tissue.
  • GelMA hydrogel was prepared by repeating the above process until GelMA was completely dissolved.
  • alginate hydrogel After putting 20mL of DW and 0.96g of alginate in a 50mL conical tube and vortexing, a 4.8% by weight alginate hydrogel was prepared by heating in a hot plate at 180 ° C for about 4 hours.
  • Calcium chloride was diluted in DW to prepare 0.1N CaCl2.
  • 40% Pluronic F-127 was added to 0.1N CaCl2 solution and vortexed.
  • a hydrogel for sacrificial formation was prepared by storing at a low temperature in a refrigerator and repeating vortexing until completely mixed. If necessary, a rotater can be used during the mixing process.
  • 3 ⁇ 3/16 Silicon tubes were cut by 9 cm and stored in a 50mL conical tube with 70% ethanol, and 17/21G coaxial nozzles and 15G plastic nozzles were prepared.
  • a 3D bioprinter from TNR Biofab was used, and a prepared silicon tube was installed by connecting it between the side protrusion of the coaxial nozzle and the plastic nozzle.
  • the hydrogel for conduit formation and the hydrogel for sacrificial formation prepared in Example 1 were loaded on the two heads provided in the 3D printer, respectively.
  • GelMA included in the hydrogel for forming a conduit is in a liquid state at high temperature and hardens close to solid when stored in a refrigerator, and the hydrogel for forming a sacrificial material exists in a liquid state at a low temperature, so each hydrogel contained in a syringe is a printable jelly. It took about 30 to 1 hour to adapt to the print head temperature until it changed to the same state.
  • the process conditions of the head loaded with the hydrogel for formation of the conduit were 26° C. and 220 to 230 kPa, and the process conditions of the head loaded with the hydrogel for formation of the sacrificial body were adjusted to 35° C. and 120 to 130 kPa. After that, the 3D printer was driven to output the pipeline structure.
  • UV irradiation was performed for 180 seconds so that the amount of light reaching the surface of the output pipeline structure was 70 mW/cm 2 .
  • a conduit having a desired shape can be obtained as a printing output, and it can be seen that it has sufficient durability to be picked up with tweezers after curing.
  • tubular biological tissue such as the straight conduit shown in FIG. 9A as well as the tubular biological tissue having the structure shown in FIGS. 8B to 8D.
  • Example 2 In order to check the UV curing of the hydrogel for tube formation obtained in Example 1, the hydrogel for tube formation was placed in two conical tubes, and UV irradiation was performed for 180 seconds so that the light intensity of one was 70 mW / cm 2, and the other One did not undergo UV irradiation.
  • Example 2 The perfusion performance of the tubular biological tissue prepared in Example 2 was evaluated using a syringe pump as follows, and the results are shown in FIGS. 9a to 10c.
  • a nozzle connected to a syringe pump was connected to the end of the hollow conduit, and the colored liquid was flowed at a constant flow rate.

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Abstract

La présente invention concerne une technologie de construction d'un tissu biologique tubulaire, tel qu'un vaisseau sanguin, un conduit nerveux et similaire et, plus spécifiquement, un kit de composition d'encre biologique pour un tissu biologique tubulaire, son procédé de fabrication, un procédé de construction d'un tissu biologique tubulaire, et un tissu biologique tubulaire construit par le procédé, un kit de composition d'encre biologique composé de deux solutions ayant des propriétés physiques différentes l'une par rapport à l'autre en raison de leurs différents composants, et des tissus biologiques tubulaires ayant diverses formes pouvant être facilement construits à l'aide de la composition d'encre biologique ayant différentes propriétés physiques dans la coque et le noyau de la buse coaxiale.
PCT/KR2022/019736 2021-12-13 2022-12-06 Kit de composition d'encre biologique pour tissu biologique tubulaire, son procédé de fabrication, procédé de construction de tissu biologique tubulaire l'utilisant, et tissu biologique tubulaire construit par ledit procédé Ceased WO2023113354A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2021-0177849 2021-12-13
KR20210177849 2021-12-13
KR1020220163090A KR20230089541A (ko) 2021-12-13 2022-11-29 관형 생체조직용 바이오잉크조성물키트, 그 제조방법, 이를 이용한 관형 생체조직 제조방법 및 그 방법으로 제조된 관형 생체조직
KR10-2022-0163090 2022-11-29

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CN119405905A (zh) * 2025-01-03 2025-02-11 宁波大学附属第一医院 一种3d同轴打印明胶复合水凝胶墨水及其制备方法和应用

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KR102180865B1 (ko) * 2019-09-30 2020-11-19 한림대학교 산학협력단 전기전도성을 갖는 광가교 바이오 잉크 조성물 및 이의 제조방법
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KR20210128785A (ko) * 2020-04-17 2021-10-27 서강대학교산학협력단 연골 모사체 제조용 바이오 잉크 조성물 및 이를 이용한 연골 모사체의 제조방법
EP3932437A1 (fr) * 2020-07-03 2022-01-05 Fundació Institut de Bioenginyeria de Catalunya (IBEC) Système d'impression permettant d'obtenir des fibres biologiques

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