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WO2022013761A1 - Système et procédé de criblage de patients infectés par le syndrome respiratoire aigu sévère du coronavirus 2 - Google Patents

Système et procédé de criblage de patients infectés par le syndrome respiratoire aigu sévère du coronavirus 2 Download PDF

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Publication number
WO2022013761A1
WO2022013761A1 PCT/IB2021/056325 IB2021056325W WO2022013761A1 WO 2022013761 A1 WO2022013761 A1 WO 2022013761A1 IB 2021056325 W IB2021056325 W IB 2021056325W WO 2022013761 A1 WO2022013761 A1 WO 2022013761A1
Authority
WO
WIPO (PCT)
Prior art keywords
cdna
sars
cov
primers
tubes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2021/056325
Other languages
English (en)
Inventor
Ruchika FERNANDO
Gayan Saranga SUMATHIPALA
Rohitha Vishvernath MUTHUGALA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Peradeniya
Original Assignee
University of Peradeniya
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Peradeniya filed Critical University of Peradeniya
Publication of WO2022013761A1 publication Critical patent/WO2022013761A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

Definitions

  • the set of one or more second primers include SEQ. ID No. 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24.
  • the SARS-CoV-2 vims infection identification in the sample is performed via Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT- LAMP).
  • RT- LAMP Reverse Transcriptase Loop-Mediated Isothermal Amplification
  • the RT-LAMP is colorimetric.
  • the sample is a tissue or a body fluid including blood, saliva, nasal drops, nasal swab, throat swab, pharyngeal swab, alveolar lavage fluid, plasma, teardrops, urine, stool, semen, or a tissue sample.
  • a "primer” refers to an oligonucleotide that can be hybridized to a complementary nucleic acid sequence and is utilized in a polymerase chain reaction for amplification of a target nucleic acid molecule.
  • Table 4 provides the composition of each 20pl reaction of the product (after adding the 5m1 of template RNA, the total reaction volume is 25m1).
  • SARS- CoV-2 RT-LAMP and the Human beta-actin RT-LAMP the same following formulation is used except for the primers.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé, un système et un kit pour détecter la présence du SRAS-CoV-2 dans un échantillon. Le procédé comprend une pluralité de premières amorces, les amorces s'hybridant au génome du SRAS-CoV-2 ou à l'ADNc correspondant. Le procédé comprend en outre une pluralité de secondes amorces, les amorces s'hybridant à l'ARNm de bêta-actine humaine ou à l'ADNc correspondant. Le système comprend une transcriptase inverse WarmStart, une ADN polymérase WarmStart Bst 2,0, une première pluralité de tubes pour doser l'ARNm du SRAS-CoV-2 ou l'ADNc correspondant ; et une seconde pluralité de tubes pour le dosage indépendant et parallèle du gène de la bêta-actine humaine ou de l'ARNm correspondant. L'invention concerne également un kit de détection du SRAS-COV-2 dans un échantillon.
PCT/IB2021/056325 2020-07-14 2021-07-14 Système et procédé de criblage de patients infectés par le syndrome respiratoire aigu sévère du coronavirus 2 Ceased WO2022013761A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
LK21222 2020-07-14
LK2122220 2020-07-14

Publications (1)

Publication Number Publication Date
WO2022013761A1 true WO2022013761A1 (fr) 2022-01-20

Family

ID=79556133

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2021/056325 Ceased WO2022013761A1 (fr) 2020-07-14 2021-07-14 Système et procédé de criblage de patients infectés par le syndrome respiratoire aigu sévère du coronavirus 2

Country Status (1)

Country Link
WO (1) WO2022013761A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115044710A (zh) * 2022-06-10 2022-09-13 广州动物园 一种检测穿山甲β冠状病毒的引物组、试剂盒和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070042350A1 (en) * 2003-07-14 2007-02-22 Ze Li Methods and compositions for detecting sars virus and other infectious agents

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070042350A1 (en) * 2003-07-14 2007-02-22 Ze Li Methods and compositions for detecting sars virus and other infectious agents

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
PARK MYUNGSUN, WON JOUNGHA, CHOI BYUNG YOON, LEE C. JUSTIN: "Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR", EXPERIMENTAL AND MOLECULAR MEDICINE, SEOUL, KR, vol. 52, no. 6, 1 June 2020 (2020-06-01), KR , pages 963 - 977, XP055851641, ISSN: 1226-3613, DOI: 10.1038/s12276-020-0452-7 *
RAFF T; VAN DER GIET M; ENDEMANN D; WIEDERHOLT T; PAUL M: "DESIGN AND TESTING OF BETA-ACTIN PRIMERS FOR RT-PCR THAT DO NOT CO-AMPLIFY PROCESSED PSEUDOGENES", BIOTECHNIQUES, INFORMA HEALTHCARE, US, vol. 23, no. 3, 1 January 1997 (1997-01-01), US , pages 456 - 458,460, XP001206667, ISSN: 0736-6205 *
WEI E. HUANG, BOON LIM, CHIA‐CHEN HSU, DAN XIONG, WEI WU, YEJIONG YU, HUIDONG JIA, YUN WANG, YIDA ZENG, MENGMENG JI, HONG CH: "RT‐LAMP for rapid diagnosis of coronavirus SARS‐CoV‐2", MICROBIAL BIOTECHNOLOGY, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 13, no. 4, 1 July 2020 (2020-07-01), GB , pages 950 - 961, XP055733462, ISSN: 1751-7915, DOI: 10.1111/1751-7915.13586 *
ZHANG YINHUA, REN GUOPING, BUSS JACKSON, BARRY ANDREW J, PATTON GREGORY C, TANNER NATHAN A: "Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride", BIOTECHNIQUES, INFORMA HEALTHCARE, US, vol. 69, no. 3, 1 September 2020 (2020-09-01), US , pages 178 - 185, XP055888013, ISSN: 0736-6205, DOI: 10.2144/btn-2020-0078 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115044710A (zh) * 2022-06-10 2022-09-13 广州动物园 一种检测穿山甲β冠状病毒的引物组、试剂盒和应用

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