WO2021080535A2

WO2021080535A2 - Memory booster essential oil mixture

Info

Publication number: WO2021080535A2
Application number: PCT/TR2020/050913
Authority: WO
Inventors: Muhammed Halis ERTAŞ
Original assignee: Talya Bitkisel Ueruenler Ticaret Ve Sanayi AS
Current assignee: Talya Bitkisel Ueruenler Ticaret Ve Sanayi AS
Priority date: 2019-10-21
Filing date: 2020-10-06
Publication date: 2021-04-29
Anticipated expiration: 2022-04-21
Also published as: TR201916207A2; WO2021080535A3

Abstract

The invention is related to an essential oil mixture comprising Orange Oil (Aurantii dulcis aetheroleum), Lemon Oil (Citrus aetheroleum), Lavender Oil (Lavandulae aetheroleum), Sage Oil (Salvia triloba aetheroleum) and Rosemary Oil (Rosmarini aetheroleum), and to the use of this mixture in strengthening memory, improving mental capacity, facilitating daily life, improving focus and concentration, and treating dementia and Alzheimer's.

Description

MEMORY BOOSTER ESSENTIAL OIL MIXTURE

Technical Field

The invention relates to a essential oil and mixture thereof comprising Orange Oil (Aurantii dulcis aetheroleum), Lemon Oil (Citrus aetheroleum), Lavender Oil (Lavandulae aetheroleum), Sage Oil (Salvia triloba aetheroleum) and Rosemary Oil (Rosmarini aetheroleum).

Prior Art

Essential oils are well-known natural ingredients and are used by means of extraction to provide odor to various preparations and formulations including perfumes, cosmetics, and foods. In topical preparations, various essential oils are used such as menthol, eucalyptus oil, camphor, peppermint for the purpose of relieving pain. Vapors from some essential oils are believed to be advantageous to remove multiple conditions, when inhaled, as in "aromatherapy". The usage of essential oils for cosmetic products known from the prior art has the principle of providing odor or flavor to the products rather than any direct effect or benefit from the essential oil itself. Although the characteristics of specific essential oils and various combinations of essential oils are substantially known, a wide range of possible combinations of essential oils have not been completely searched yet.

Various foods such as fish, coffee, blueberry, turmeric, broccoli, pumpkin seeds, bitter chocolate, dried fruits, orange, and eggs have been offered for the purpose of enhancing mental activities such as memory and concentration and maintaining brain health.

Various researches have been carried out regarding the clinical benefits of essential oils. Aromatherapy can enhance the quality of life and it can even function as a complementary treatment to reduce the negative effects of various conditions such as stress, pain, and depression.

CN109805409 (Wang) has suggested a mixture of soy lecithin, walnut oil, perilla oil, linseed oil, almond oil, sunflower seed oil, safflower oil, acerola oil, shasnong seed oil and peony seed oil as a memory booster.

CN109602010 (Xu) has referred to a nutritious mixture comprising phosphatidylserine, krill oil and galangal plants with sharp leaves. CN109497537 (Tang) has suggested a mixture of water-soluble flax powder, royal jelly, cysteamine and in addition acid grain flour, vitamin Bl, vitamin B12, ferrous salt, dextrin, antioxidants to enhance brain functions and to ease down brain atrophy and reprieve aging. CN108902937 (He) has disclosed a composition comprising, flax seed kernel, Tianma, longan, Yuanzhi, Yizhiren, Ginkgo biloba extract, grape seed extract, selenium yeast, zinc yeast, magnesium lactate, vitamin B and acetylcholine for treating senile dementia and Alzheimer's disease.

All of these are nutritious mixtures are taken by digestion.

US2019083564 (Harris) disclosed the use of a mixture of essential oils such as Vetiver, Black Pepper, Ginger, Ylang Ylang, Sandalwood, Patchouli, Cedarwood, Myrrh, Frankincense, Lavender for neurological disorders such as seizures, migraines, anxiety, and tremors. The proposed mixture to improve cognitive functions may also contain Coffee, Vanilla, Cinnamon Bark, Cloves, Dalmatian Sage, Rosemary, Blood Orange, Lemon, Grapefruit, Peppermint, Lime, Clementine, Spearmint or Coconut oil. Although the composition is specified to be administered by aromatherapy, it is essentially applied to the skin.

CN104800774 A (Xia) relates to a mixture containing 2-8 moiety lavender essential oil, 2-8 moiety tulip essential oil, 1-5 moiety rosemary essential oil and 1-5 moiety jasmine essential oil, which improves sleep quality.

US2007031343 (Bonfour) discloses a large number of fragrance oils that can be used in inhalant formulations containing an active substance, carrier agent, and effective amounts of fragrance. US20160279358 (Singer) relates to an inhalation rod comprising at least two essential oils. Currently, it is clear that there is a need for essential oil mixtures, which are especially applied by way of inhalation, that are used in strengthening the memory, improving mental capacity, facilitating daily life, increasing focus and concentration, and treating dementia and Alzheimer's disease.

Brief Description of the Invention

The invention is related to an essential oil mixture comprising Orange Oil (Aurantii dulcis aetheroleum), Lemon Oil (Citrus aetheroleum), Lavender Oil (Lavandulae aetheroleum), Sage Oil (Salvia triloba aetheroleum) and Rosemary Oil (Rosmarini aetheroleum), and to the use of this mixture in strengthening memory, improving mental capacity, facilitating daily life, improving focus and concentration, and treating dementia and Alzheimer’s. Detailed Description of the Invention

The invention is an essential oil mixture; comprising Orange Oil (Aurantii dulcis aetheroleum), Lemon Oil (Citrus aetheroleum), Lavender Oil (Lavandulae aetheroleum), Sage Oil (Salvia triloba aetheroleum) and Rosemary Oil (Rosmarini aetheroleum). The essential oils used in the composition of the invention are compatible with each other and there is no incompatibility between them. Essential oils provide a synergistic effect in the developed formulation.

In another embodiment of the invention, the essential oil mixture comprising Orange Oil (Aurantii dulcis aetheroleum), Lemon Oil (Citrus aetheroleum), Lavender Oil (Lavandulae aetheroleum), Sage Oil (Salvia triloba aetheroleum) and Rosemary Oil (Rosmarini aetheroleum) is used for strengthening the memory, improving mental capacity, facilitating daily life, increasing focus and concentration, and treating dementia and Alzheimer's disease.

Orange oil and lemon oil used in the mixture of the present invention can be obtained by compressing fresh fruit peel; lavender oil, sage oil, and rosemary oil steam distillation.

Orange oil is a liquid with a specific weight of 0.8-1.05 g/cm and a refractive index of 1.46546- 1.47037. It contains at least 90% by weight d-limonene. Lemon oil is a liquid with a specific weight of 0.8-1.05 g/cm and a refractive index of 1.46546-1.47037. Lemon oil is obtained from the fresh peel of the fruit. It contains citral, d-limonene, pinene, campen, b-felandren and g- terpinen as terpenic compounds. Lavender oil is a yellow liquid with a specific weight of 0.878-

1.05 g/cm and a refractive index of 1.455-1.488. Sage oil is a yellow liquid with a specific weight of 0.9-1.05 g/cm and a refractive index of 1,46061-1,46546. Rosemary Oil is a yellow liquid with a specific weight of 0.8-1.05 g/cm and a refractive index of 1.46061 - 1.46546. It contains 19-25% by weight cineol.

The essential oils in the essential oil mixture of the present invention may preferably be in the proportions given below. The value stated in the invention as by weight is defined as the weight of the essential substance/the weight of the total mixture.

Essential oil mixture contains preferably 60-75% by weight, more preferably 65-70% by weight, most preferably 68% by weight Orange Oil (Aurantii dulcis aetheroleum). Essential oil mixture contains preferably 20-40% by weight, more preferably 25-35% by weight, most preferably 30% by weight Lemon Oil (Citrus aetheroleum).

Essential oil mixture contains preferably 0,5-3% by weight, more preferably 0,5-2% by weight, most preferably 1% by weight Lavender Oil (Lavandulae aetheroleum).

Essential oil mixture contains preferably 0,25-2% by weight, more preferably 0,25-1% by weight, most preferably 0,5% by weight Sage Oil (Salvia triloba aetheroleum).

Essential oil mixture contains preferably 0,25-2% by weight, more preferably 0,25-1% by weight, most preferably 0,5% by weight Rosemary Oil (Rosmarini aetheroleum).

In an embodiment of the invention, the essential oil mixture contains 65-70% by weight Orange Oil (Aurantii dulcis aetheroleum), 25-35% by weight Lemon Oil (Citrus aetheroleum), 0,5-2% by weight Lavender Oil (Lavandulae aetheroleum), 0,25-1% by weight Sage Oil (Salvia triloba aetheroleum), 0,25-1% of Rosemary Oil (Rosmarini aetheroleum).

In another embodiment of the invention, the essential oil mixture contains 68% by weight Orange Oil (Aurantii dulcis aetheroleum), 30% by weight Lemon Oil (Citrus aetheroleum), 1% by weight Lavender Oil (Lavandulae aetheroleum), 0,5% by weight Sage Oil (Salvia triloba aetheroleum), 0,5% of Rosemary Oil (Rosmarini aetheroleum).

Essential oil mixtures in all embodiments of the invention can be used to strengthen memory, improve mental capacity, facilitate daily life, improve focus and concentration, treat dementia and Alzheimer’s.

The inventive essential oil mixture can be in the form of a solution, aerosol, spray, flavor or nasal inhaler.

The inventive essential oil mixture can be applied by inhalation orally and/or nasally. It can be applied directly through the nasal route or by sniffing a napkin that is damp with the mixture that is placed on an item such as a pillow, or through inhalation. Specifically, the essential oil mixture can be dripped on the person’s dress, for example on the collar and can be applied through sniffing. Application is preferably performed 3 or 4 times a day. The essential oil mixture according to the present invention may have a refractive index of 1.4679 - 1.4704 at 25°C, a specific weight of 0,850-1,05 g/cm3 at 25°C.

According to the invention, the following exemplary combinations are possible.

Essential oils can comprise various compounds in their terpene structure. The inventive essential oil mixture that comprises Orange Oil, Lemon Oil, Lavander Oil, Sage Oil, and Rosemary Oil can comprise Limonene, a-Pinene, b-pinene, Sabinene, b-Myrcene, jfTcrpincnc, Cymene, Linalool, Valencene from the terpene class compounds.

In the inventive essential oil mixture, the amount of Limonene is preferably at least 80% by weight, more preferably at least 85% by weight, most preferably at least 90% by weight. In the inventive essential oil mixture, the amount of each a-Pinene, b-pinene, b-Myrcene, y¹ Terpinene compounds is at least 0,25% by weight, preferably at least 0,5% by weight, most preferably at least 0,1% by weight.

In the inventive essential oil mixture, the amount of each Sabinene, Cymene, Linalool, Valencene compounds is at least 0,25% by weight.

The analysis of the inventive mixture is carried out by GC/GC-MS. In the process, Gas chromatography (Agilent 7890A)- mass detector (Agilent 5975C) device or capillary column (HP InnowaxCapillary; 60.0 m x 0.25 mm x 0.25 pm) with an equivalent device or equivalent column is used. The samples are diluted with hexane with a ratio of 1:100 for analysis. In the analysis, helium having a 0,8 ml/min flow rate is used as a carrier gas. The samples are injected into the device in 1 pi with a 40:1 split ratio. The temperature of the injector is maintained at 250°C, column temperature program is adjusted to be 60°C (10 minutes), from 60°C to 220°C, 4°C/minutes and 220°C (10 minutes). In this temperature program, the total analysis time is about 60 minutes. For the mass detector, scan range (m/z) 35-450 atomic mass unit and electron bombardment ionization 70 eV are used. In the identification of the compounds of the essential oils, the data of WILEY and OIL ADAMS libraries have been taken into account. Component percentages of the results have been carried out with the FID detector, and identification of components have been conducted with the MS detector. Tests:

The tests were performed on the following sample (MO or Motivate):

a) la vitro enzyme inhibition tests In order to reveal the memory boosting effect of the essential oil mixture, in vitro enzyme inhibition tests were carried out with the sample (MO). Tested enzymes are the enzymes called acetylcholine esterase and butyrylcholine esterase which play a role in the pathogenesis of Alzheimer’s disease and nowadays, cholinesterase inhibitors are the most prescribed drug group in the treatment of disease. Therefore the product was tested against cholinesterase enzymes. In the enzyme inhibition test, the Ellman method was implemented. The results obtained are given below:

a Standard deviation (n=3), b Concentration, ^c Concentration of Reference Drug: 1 mg/ml b) In vivo tests

In order to test the memory strengthening effect of the essential oil mixture, in vivo experiments were carried out with the sample (MO). For this purpose, 4-months old, 35 Wistar-type rats with weighing 350 g (± 10 g) were used. The rats were divided into 7 experimental groups, each consisting of 5 rats and the groups were arranged as follows:

• Control group: Scopolamine (Sco, 0.7 mg/kg body weight, i.p.) were given by inhalation in essential oil mixture (MO) (1% MO + Sco and 3% MO + Sco). · MO treatment groups (1% MO and 3% MO)

• Donepezil group (positive control, 5 mg/kg body weight, i.p.)

Tests below were carried out on rats as behavioral experiment models.

Y-Maze test With the Y-maze test developed by Jackson (1943) and Postu et al. (2018), the effect of the essential oil sample (MO) given to the rats via inhalation with the ratio of 1% and 3% were measured in relation to memory enhancement, in the maze consisting of arms, each of which has 25 cm height, 35 cm length, and 10 cm width. (Figure 1) Radial (Annular) Arm-Maze Test

With the radial (annular) arm-maze test developed by Olton & Samuelson (1976) and Postu et al. (2018), it is intended to measure spatial (dimensional) memory and locomotor activity. There is food at the end of the arms numbered 1, 2, 4, 5 and 7 belonging to the maze with 8 arms having 32 cm diameter. Firstly the rats are adapted to the environment, they are taught to go to the end of arms of the maze and it has been ensured that they were attracted to the feed pellets for 5 minutes. A trial period for one day was given to the rats. The effect of the essential oil sample (MO) provided to the rats via inhalation with the ratio of 1% and 3% on memory (working memory and reference memory mistakes) were measured this way (Figure 2).

Results:

Scopolamine injection significantly reduced short-term memory performance, as evidenced by a decrease in the percentage of spontaneous changes compared to the control group (p <0.01). Both MO doses, especially the one with higher (3%) scopolamine, reduced the percentage of spontaneous changes (p <0.001). The reference drug donepezil and both MO doses (1% and 3%) significantly increased the spontaneous alternative percentage, close to the control group, indicating its positive effects on short-term memory (Figure 1). However, the effect of MO on the development of short-term memory, could not be attributed to locomotor activity as explained in the number of maze arm entries, since no significant difference was found between the groups (Figure 2).

Both MO doses (especially 1%) showed a significant reduction in the number of scopolamine-induced working memory errors compared to rats that were administered scopolamine alone. As expected, the reference drug donepezil and both MO doses (1% and 3%) indicate positive effects on working memory, significantly affecting the number of working memory errors close to the control group (Figure 3).

In addition, according to the scopolamine (p <0.001) (Figure 4) group, which is used to significantly disrupt the reference memory; both MO doses significantly reduced the number of reference memory errors compared to rats given scopolamine (p <0.001). Finally, the reference drug donepezil and both MO doses (1% and 3%) improved reference memory compared to the control group (p <0.0001).

Experimental results obtained in vitro and in vivo; indicate that Motivate (MO), which is the essential oil mixture of the invention, has positive effects on memory and learning. Description of the figures:

Figure 1. Percentage of spontaneous change of the essential oil sample (MO) given to rats via inhalation at a rate of 1% and 3% by Y-maze test

Figure 2. Number of entry into the maze arms of the locomotor activity of the essential oil sample (MO) given to rats by inhalation at the rate of 1% and 3% in the Y-maze test

Figure 3. Measurement of essential oil (MO) given to rats by inhalation at the rate of 1% and 3%, over memory errors working in radial-maze test

Figure 4. Measurement of essential oil (MO) given to rats by inhalation at a rate of 1% and 3%, over the reference memory errors in the radial-maze test

Claims

1. An essential oil mixture; comprising Orange oil (Aurantii dulcis aetheroleum), Lemon Oil (Citrus aetheroleum), Lavander Oil (Lavandulae aetheroleum), Sage Oil (Salvia triloba aetheroleum), Rosemary Oil (Rosmarini aetheroleum).

2. An essential oil mixture according to claim 1; comprising 60-75% by weight, preferably 65- 70% by weight, more preferably 68% by weight Orange Oil (Aurantii dulcis aetheroleum).

3. An essential oil mixture according to claim 1; comprising 20-40% by weight, preferably 25- 35% by weight, more preferably 30% by weight Lemon Oil (Citrus aetheroleum).

4. An essential oil mixture according to claim 1; comprising 0,5-3% by weight, preferably 0,5-

2% by weight, more preferably 1% by weight Lavender Oil (Lavandulae aetheroleum).

5. An essential oil mixture according to claim 1; comprising 0,25-2% by weight, preferably 0,25-1% by weight, more preferably 0,5% by weight Sage Oil (Salvia triloba aetheroleum).

6. An essential oil mixture according to claim 1; comprising 0,25-2% by weight, preferably 0,25-1% by weight, more preferably 0,5% by weight Rosemary Oil (Rosmarini aetheroleum).

7. An essential oil mixture according to claim 1; comprising 65-70% by weight Orange Oil (Aurantii dulcis aetheroleum), 25-35% by weight Lemon Oil (Citrus aetheroleum), 0,5-2% by weight Lavender Oil (Lavandulae aetheroleum), 0,25-1% by weight Sage Oil (Salvia triloba aetheroleum), 0,25-1% by weight Rosemary Oil (Rosmarini aetheroleum).

8. An essential oil mixture according to claim 7; comprising approximately 68% by weight

Orange Oil (Aurantii dulcis aetheroleum), approximately 30% by weight Lemon Oil (Citrus aetheroleum), approximately 1% by weight Lavender Oil (Lavandulae aetheroleum), approximately 0,5% by weight Sage Oil (Salvia triloba aetheroleum), approximately 0,5% by weight Rosemary Oil (Rosmarini aetheroleum).

9. An essential oil mixture according to any of the preceding claims for use to boost memory, improve mental capacity, facilitate daily life, improve focus and concentration, and treat dementia and Alzheimer’s.

10. An essential oil mixture according to any of the preceding claims, wherein the mixture is applied by inhalation orally and/or nasally.

11. An essential oil mixture according to any of the preceding claims, wherein the mixture is in the form of a solution, an aerosol, spray, an aroma or a nasal inhaler.