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WO2021066464A3 - 핵산 검출 시스템 - Google Patents

핵산 검출 시스템 Download PDF

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Publication number
WO2021066464A3
WO2021066464A3 PCT/KR2020/013234 KR2020013234W WO2021066464A3 WO 2021066464 A3 WO2021066464 A3 WO 2021066464A3 KR 2020013234 W KR2020013234 W KR 2020013234W WO 2021066464 A3 WO2021066464 A3 WO 2021066464A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
target nucleic
detection system
acid detection
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2020/013234
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English (en)
French (fr)
Other versions
WO2021066464A2 (ko
Inventor
엄숭호
육지수
김정훈
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Progeneer Inc
Original Assignee
Progeneer Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Progeneer Inc filed Critical Progeneer Inc
Publication of WO2021066464A2 publication Critical patent/WO2021066464A2/ko
Publication of WO2021066464A3 publication Critical patent/WO2021066464A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/155—Particles of a defined size, e.g. nanoparticles

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 표적 핵산을 단일화된 특정 서열을 갖는 유니버셜 코드로 변환하여 검출 및 증폭하는 방법에 관한 것으로, 본 발명에 따르면, 무효소 반응으로 표적 핵산을 검출하거나 검출 신호를 증폭할 수 있으며, 다양한 표적 핵산을 미리 지정해둔 유니버셜 코드로 전환/단일화할 수 있으므로, 표적 핵산 각각에 대한 프로브를 매번 새로 디자인할 필요가 없어, 복잡한 구조의 검출 시스템에 유용하게 적용할 수 있다.
PCT/KR2020/013234 2019-10-04 2020-09-28 핵산 검출 시스템 Ceased WO2021066464A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20190122875 2019-10-04
KR10-2019-0122875 2019-10-04

Publications (2)

Publication Number Publication Date
WO2021066464A2 WO2021066464A2 (ko) 2021-04-08
WO2021066464A3 true WO2021066464A3 (ko) 2021-05-27

Family

ID=75338337

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2020/013234 Ceased WO2021066464A2 (ko) 2019-10-04 2020-09-28 핵산 검출 시스템

Country Status (2)

Country Link
KR (1) KR102375106B1 (ko)
WO (1) WO2021066464A2 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102821861B1 (ko) * 2021-11-05 2025-06-17 서울대학교산학협력단 나노입자를 이용한 표적물질의 증폭 또는 검출
JP2024540274A (ja) * 2021-11-05 2024-10-31 ソウル大学校産学協力団 ナノ粒子を利用した標的物質の増幅または検出方法
KR102632594B1 (ko) * 2022-08-04 2024-02-01 한국생명공학연구원 유전자 검출 신호 증폭이 가능한 신개념 다중 유전자 진단 기술
CN116328744B (zh) * 2023-05-15 2024-04-26 哈尔滨工程大学 一种高吸附选择性抗污损型氧化石墨烯铀吸附材料及其制备方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150055016A (ko) * 2012-09-11 2015-05-20 유니센스 다이아그노스틱스 앱스 가닥 치환 교환 반응을 이용한 비-핵산 피분석물의 검출
KR20190081363A (ko) * 2017-12-29 2019-07-09 인천대학교 산학협력단 금 나노입자 결합 핵산 나노구조체를 이용한 신호증폭 및 응용 방법
KR20190086259A (ko) * 2018-01-12 2019-07-22 성균관대학교산학협력단 핵산 검출용 형광핵산나노구조체-그래핀 바이오센서

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US20110136116A1 (en) 2008-04-08 2011-06-09 Cornell University Detection of target nucleic acid sequences using fluorescence resonance energy transfer
US8940487B2 (en) 2010-02-09 2015-01-27 UmiTaq Bio Methods and compositions for universal detection of nucleic acids
CN109715825B (zh) * 2016-05-16 2023-01-06 纳米线科技公司 用于检测样品中目标核酸的方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
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KR20150055016A (ko) * 2012-09-11 2015-05-20 유니센스 다이아그노스틱스 앱스 가닥 치환 교환 반응을 이용한 비-핵산 피분석물의 검출
KR20190081363A (ko) * 2017-12-29 2019-07-09 인천대학교 산학협력단 금 나노입자 결합 핵산 나노구조체를 이용한 신호증폭 및 응용 방법
KR20190086259A (ko) * 2018-01-12 2019-07-22 성균관대학교산학협력단 핵산 검출용 형광핵산나노구조체-그래핀 바이오센서

Non-Patent Citations (2)

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Title
HU MUREN, MAO DONGSHENG, LIU XIAOHAO, REN LINGJIE, ZHOU MENGRU, CHEN XIAOXIA, ZHU XIAOLI: "An all-in-one homogeneous DNA walking nanomachine and its application for intracellular analysis of miRNA", THERANOSTICS, IVYSPRING INTERNATIONAL PUBLISHER, AU, vol. 9, no. 20, 1 January 2019 (2019-01-01), AU, pages 5914 - 5923, XP055813569, ISSN: 1838-7640, DOI: 10.7150/thno.36081 *
QIONG LI, ZHI LIU, DANHUA ZHOU, JIAFENG PAN, CHENGSHUAI LIU, JUNHUA CHEN: "A cascade toehold-mediated strand displacement strategy for label-free and sensitive non-enzymatic recycling amplification detection of the HIV-1 gene", ANALYST, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 144, no. 6, 11 March 2019 (2019-03-11), UK, pages 2173 - 2178, XP055645131, ISSN: 0003-2654, DOI: 10.1039/C8AN02340A *

Also Published As

Publication number Publication date
WO2021066464A2 (ko) 2021-04-08
KR20210040791A (ko) 2021-04-14
KR102375106B1 (ko) 2022-03-17

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