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GB202219233D0 - Magnetic bead technology system for amplifying signal of nucleic acid detection based on crispr technology, and use thereof - Google Patents

Magnetic bead technology system for amplifying signal of nucleic acid detection based on crispr technology, and use thereof

Info

Publication number
GB202219233D0
GB202219233D0 GBGB2219233.0A GB202219233A GB202219233D0 GB 202219233 D0 GB202219233 D0 GB 202219233D0 GB 202219233 A GB202219233 A GB 202219233A GB 202219233 D0 GB202219233 D0 GB 202219233D0
Authority
GB
United Kingdom
Prior art keywords
technology
nucleic acid
magnetic bead
detection based
acid detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
GBGB2219233.0A
Other versions
GB2632620A (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Suzhou Vertex Biological Pharmaceutical Co Ltd
Original Assignee
Suzhou Vertex Biological Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou Vertex Biological Pharmaceutical Co Ltd filed Critical Suzhou Vertex Biological Pharmaceutical Co Ltd
Publication of GB202219233D0 publication Critical patent/GB202219233D0/en
Publication of GB2632620A publication Critical patent/GB2632620A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/143—Magnetism, e.g. magnetic label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/519—Detection characterised by immobilisation to a surface characterised by the capture moiety being a single stranded oligonucleotide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G01N2333/938—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
GB2219233.0A 2020-08-10 2021-10-09 Magnetic bead technology system for amplifying signal of nucleic acid detection based on CRISPR technology, and use thereof Pending GB2632620A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202010793936.XA CN111850097B (en) 2020-08-10 2020-08-10 Signal amplification magnetic bead technology system for nucleic acid detection based on CRISPR technology and its application
PCT/CN2021/122789 WO2022033607A2 (en) 2020-08-10 2021-10-09 Magnetic bead technology system for amplifying signal of nucleic acid detection based on crispr technology, and use thereof

Publications (2)

Publication Number Publication Date
GB202219233D0 true GB202219233D0 (en) 2023-02-01
GB2632620A GB2632620A (en) 2025-02-19

Family

ID=72972666

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2219233.0A Pending GB2632620A (en) 2020-08-10 2021-10-09 Magnetic bead technology system for amplifying signal of nucleic acid detection based on CRISPR technology, and use thereof

Country Status (3)

Country Link
CN (1) CN111850097B (en)
GB (1) GB2632620A (en)
WO (1) WO2022033607A2 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111850097B (en) * 2020-08-10 2021-04-16 苏州顶点生物医药有限公司 Signal amplification magnetic bead technology system for nucleic acid detection based on CRISPR technology and its application
KR102738301B1 (en) * 2020-11-02 2024-12-04 주식회사 이지다이아텍 Microparticle probe for nucleic acid separation and detection for multiplexed diagnosis
CN112501256A (en) * 2020-12-03 2021-03-16 台州市中心医院（台州学院附属医院） CRSPR-cas13a driven RNA rapid detection method based on double-enzyme signal amplification strategy
CN112813199B (en) * 2021-01-14 2022-07-15 青岛科技大学 Method for detecting enterovirus CVB3
CN113063936B (en) * 2021-04-02 2023-05-16 青岛农业大学 Dual signal amplification mycotoxin detection method and detection kit
CN114609104B (en) * 2022-03-11 2025-07-25 济南大学 Optical biosensor for detecting exosome based on double-rolling-ring separation
CN115141826B (en) * 2022-06-01 2024-01-05 安徽省农业科学院畜牧兽医研究所 RPA primer pair and application thereof, kit for visually detecting PCV4, application of kit and method for detecting PCV4
CN115537472B (en) * 2022-06-30 2023-07-14 华中科技大学协和深圳医院 Kit, method and application for rapidly detecting human bocavirus type 1 nucleic acid
CN116144738B (en) * 2022-08-24 2023-08-08 北京科技大学 CRISPR/Cas12a sensing system enhanced by single microsphere spatial confinement and its application
CN116179648A (en) * 2022-12-01 2023-05-30 深圳高性能医疗器械国家研究院有限公司 Reporter magnetic beads, preparation method and application thereof, detection kit and detection method
CN116024304A (en) * 2022-12-01 2023-04-28 深圳高性能医疗器械国家研究院有限公司 Reporter magnetic bead composition, preparation method and application thereof, detection kit and detection method
CN115786464A (en) * 2022-12-01 2023-03-14 深圳高性能医疗器械国家研究院有限公司 Reporter nucleic acid composition, preparation method and application thereof, detection kit and detection method
CN116004919B (en) * 2022-12-15 2025-03-21 中国科学院深圳先进技术研究院 Virus nucleic acid detection reagent, kit, preparation method and application thereof
CN116287467A (en) * 2023-03-30 2023-06-23 中国人民解放军军事科学院军事医学研究院 Electrochemical biosensor based on CRISPR/Cas and its application in nucleic acid detection
CN116660345B (en) * 2023-04-25 2024-09-06 苏州中科苏净生物技术有限公司 Electrochemical nucleic acid detection sensing element based on CRISPR/Cas and detection method
CN116287133A (en) * 2023-05-16 2023-06-23 苏州中星医疗技术有限公司 Ribonucleic acid biosensor, and preparation method and application thereof
CN116990499B (en) * 2023-08-03 2024-03-01 西安交通大学 MBs-ssDNA-hCG probe, kit and application thereof in detection of IAP
CN117947195B (en) * 2024-02-20 2024-11-22 江苏省家禽科学研究所 One-step CRISPR/Cas12b detection kit and method for detecting Salmonella
CN118374641B (en) * 2024-06-24 2024-09-20 江苏省中国科学院植物研究所 Method for detecting African swine fever by using spherical nucleic acid substrate enhanced CRISPR-Cas12a system
CN118600064B (en) * 2024-08-07 2025-02-18 南昌大学第一附属医院 Detection method of high-virulence drug-resistant klebsiella pneumoniae
CN118932096A (en) * 2024-10-12 2024-11-12 国科大杭州高等研究院 Detection method of foodborne pathogens based on acoustic convergence effect
CN119269599A (en) * 2024-11-08 2025-01-07 江苏大学 A magnetic self-assembled OPN protein detection nanoprobe and its construction method and application
CN119639902A (en) * 2025-01-09 2025-03-18 重庆医科大学附属第二医院 Probe set for detecting soluble PD-L1 in peripheral blood and application thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111118218B (en) * 2020-01-20 2024-01-23 杭州奥盛仪器有限公司 Isothermal detection primer set, kit and detection method for CRISPR-Cas12a protease of prawn iridovirus
CN111270012B (en) * 2020-03-10 2021-02-02 中国人民解放军军事科学院军事医学研究院 CRISPR nucleic acid detection kit for detecting novel coronavirus (2019-nCoV)
CN111500792A (en) * 2020-06-11 2020-08-07 亚能生物技术（深圳）有限公司 Novel coronavirus detection kit
CN111850097B (en) * 2020-08-10 2021-04-16 苏州顶点生物医药有限公司 Signal amplification magnetic bead technology system for nucleic acid detection based on CRISPR technology and its application

Also Published As

Publication number Publication date
WO2022033607A2 (en) 2022-02-17
CN111850097B (en) 2021-04-16
WO2022033607A3 (en) 2022-03-24
CN111850097A (en) 2020-10-30
GB2632620A (en) 2025-02-19

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