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WO2021065831A1 - Récipient - Google Patents

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Publication number
WO2021065831A1
WO2021065831A1 PCT/JP2020/036731 JP2020036731W WO2021065831A1 WO 2021065831 A1 WO2021065831 A1 WO 2021065831A1 JP 2020036731 W JP2020036731 W JP 2020036731W WO 2021065831 A1 WO2021065831 A1 WO 2021065831A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
recess
guide thread
hair follicle
wall portion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2020/036731
Other languages
English (en)
Japanese (ja)
Inventor
久 坂井
恭介 岸本
悠 森
孝 辻
尚一 岡本
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyocera Corp
Organ Technologies Inc
RIKEN
Original Assignee
Kyocera Corp
Organ Technologies Inc
RIKEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyocera Corp, Organ Technologies Inc, RIKEN filed Critical Kyocera Corp
Priority to JP2021551269A priority Critical patent/JP7372621B2/ja
Publication of WO2021065831A1 publication Critical patent/WO2021065831A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus

Definitions

  • This disclosure relates to a container for storing regenerated hair follicle primordium.
  • the epithelial stem cells and the mesenchymal stem cells are separated from the hair follicles, and a guide thread is inserted into the aggregated cell population consisting of these stem cells to produce a regenerated hair follicle primordia, and the regenerated hair follicle primordia is used as a patient.
  • a treatment method for transplanting to the site of alopecia see, for example, Patent Document 1).
  • a large amount of regenerated hair follicle primordia can be produced from a small amount of sample by culturing epithelial stem cells and mesenchymal stem cells separated from the hair follicle organ.
  • the total number of hair follicles on the patient's scalp can be increased without increasing invasiveness.
  • the container according to the embodiment of the present disclosure includes a recess opened upward, a plurality of guide threads arranged in the recess, and a plurality of regenerating hair follicles arranged at the tips of the plurality of guide threads. It is characterized by including a primordium and a plurality of support portions for supporting the plurality of guide threads.
  • FIG. 5 is a cross-sectional view of the container shown in FIG. 1 in a state where a lid portion is attached to a container main body portion. It is a perspective view which shows the internal structure of the concave part of the container main body part shown in FIG. It is a top view which shows the internal structure of the concave part of the container main body part shown in FIG. It is sectional drawing of the container which concerns on 2nd Embodiment of this disclosure. It is a perspective view of the container main body part shown in FIG. It is a top view of the container body part shown in FIG.
  • a plurality of regenerated hair follicle progenitors are stored and transported in order to transport the produced regenerated hair follicle progenitor to the treatment site to be transplanted to the alopecia site of the patient.
  • the purpose of the present disclosure is to provide a container that can store and transport a plurality of regenerated hair follicle primordium.
  • a container capable of storing a plurality of regenerated hair follicle primordia, observing them, and transporting them while maintaining the quality.
  • the container 1 according to the first embodiment of the present disclosure shown in FIG. 1 has a container main body portion 10 and a lid portion 30.
  • the container body 10 and the lid 30 are, for example, transparent polystyrene, Teflon (registered trademark), polypropylene, polyvinyl chloride, polyethylene, cyclic polyolefin, polyethylene terephthalate, polycarbonate, polydimethylsiloxane, polymethylmethacrylate, polyarylate, respectively. It can be made of a synthetic resin material such as polysulfone, polyethersulfone, polyetheretherketone, polyetherimide, polymethylpentene, silicon resin, acrylic, PFA, or a transparent material such as transparent glass. , The material can be changed in various ways.
  • the container body portion 10 and the lid portion 30 may be formed of the same material as each other, or may be formed of different materials from each other.
  • the container body 10 has a recess 11 that opens upward.
  • the container main body 10 has a plurality (10) recesses 11 arranged side by side so as to be isolated from each other. Each recess 11 can accommodate the regenerated hair follicle primordium 2.
  • Regenerated hair follicle primordium 2 is arranged inside each of the plurality of recesses 11.
  • the regenerated hair follicle primordium 2 is arranged at the tip of the guide thread 3 and integrated with the guide thread 3, and is housed inside the recess 11 together with the guide thread 3.
  • the guide thread 3 penetrates the regenerated hair follicle primordium 2.
  • the regenerated hair follicle primordium 2 stored in the container 1 is composed of a cell population containing a plurality of epithelial cells 2a and a cell population containing a plurality of mesenchymal cells 2b.
  • the cell population containing the plurality of mesenchymal cells 2b is arranged on the distal end side (left side in FIG. 2) of the guide thread 3 rather than the cell population containing the plurality of epithelial cells 2a.
  • Epithelial cells means cells collected from epithelial tissue and cells obtained by culturing the collected cells, for example, cells collected from the outermost layer of the outer root sheath of the hair sac-derived bulge region (derived from the bulge region). Epithelial cells), cells collected from the epithelium of adult or fetal skin, epithelial cells derived from pluripotent stem cells, hair follicles, epithelial precursor cells that may differentiate into epithelial stem cells that make up the skin Such stem cells can be mentioned.
  • Mesenchymal cells means cells collected from mesenchymal tissue and cells obtained by culturing the collected cells, for example, cells derived from hair papilla (for example, hair papilla cells), hair matrix cells, and the like. Free of hair root sheath cells, inner hair root sheath cells, outer hair root sheath cells (excluding cells in the outermost layer), adult or fetal skin mesenchymal cells, mesenchymal cells derived from pluripotent stem cells, and blood cells Mesenchymal cells, mesenchymal cells derived from bone marrow, mesenchymal cells inside the jawbone, mesenchymal cells derived from head nerve ridge cells, or mesenchymal progenitor cells or stem cells that may differentiate into these cells, etc. be able to.
  • the “pluripotent stem cell” means a cell having the ability to differentiate into various tissues of the living body, for example, ES cell (Embryonic stem cell), ntES cell (nuclear transfer Embryonic stem cell), EG cell (Embryonic stem cell), EG cell (Embryonic stem cell), EG cell (Embryonic stem cell) cels), GS cells (germlyonic stem cells), iPS cells (induced Puripotent Stem cells).
  • the regenerated hair follicle primordium 2 may have a configuration other than the above. Further, the regenerated hair follicle primordium 2 can be produced, for example, by the method described in Patent Document 1.
  • the guide thread 3 is formed in a thread form (fibrous form) by, for example, a polymer such as nylon, a metal such as stainless steel, a chemical fiber such as carbon fiber or glass fiber, or the like.
  • the guide thread 3 is made of nylon.
  • the length of the guide thread 3 can be 3 to 10 mm, and the diameter of the guide thread 3 can be 0.02 to 1.20 mm. In the present embodiment, the length of the guide thread 3 is 5 mm, and the diameter of the guide thread 3 is 0.5 mm.
  • each recess 11 is filled with the culture solution 4, and the plurality of regenerated hair follicle primordiums 2 are immersed in the culture solution 4.
  • the culture solution 4 is a liquid prepared for culturing the regenerated hair follicle primordium 2.
  • the culture medium 4 may be a basal medium used for culturing animal cells, and examples thereof include Dulbecco's modified Eagle's medium (DMEM) and Advanced DMEM / F12 medium. Further, in order to efficiently proliferate epidermal cells or mesenchymal cells, cell growth factors such as serum, FGF (Fibroblast growth factor), and EGF (Epidermal growth factor) can be added.
  • Y27632, Noggin, SAG, or the like may be added as an additive for maintaining the function of cells, and antibiotics such as Penicillin and Streptomycin may be added as needed.
  • antibiotics such as Penicillin and Streptomycin
  • the basal medium, serum, cell growth factors, and additives are appropriately changed depending on the properties of epithelial cells or mesenchymal cells.
  • the container 1 has a holding structure 5 for holding the regenerated hair follicle primordium 2 in a predetermined position inside each recess 11.
  • the configurations of the holding structures 5 provided in the respective recesses 11 are the same as each other.
  • the shape of the recess 11 and the configuration of the holding structure 5 provided inside the recess 11 will be described below.
  • the recess 11 is partitioned by, for example, a bottom surface 11a having a rectangular outer shape and four inner wall surfaces 11b, 11c, 11d, and 11e that are perpendicularly connected to the edge of the bottom surface 11a.
  • the opening of the recess 11 has a rectangular shape in which the distance between the inner wall surface 11b and the inner wall surface 11e is larger than the distance between the inner wall surface 11c and the inner wall surface 11d. It has become.
  • the recess 11 is not limited to the above configuration as long as it can accommodate the regenerated hair follicle primordium 2.
  • the area of the bottom surface 11a may be 10 to 200 mm 2 , and the heights of the inner wall surfaces 11b, 11c, 11d, 11e may be set to 3 to 10 mm. Further, the volume of the recess 11 may be set to , for example, 30 to 2000 mm 3.
  • the direction perpendicular to the bottom surface 11a of the container body 10 and the direction from the bottom surface 11a toward the opening of the recess 11 is upward, and the opposite is downward.
  • the longitudinal direction of the guide thread 3 is parallel to the bottom surface 11a and perpendicular to the inner wall surfaces 11b and 11e, and the regenerated hair follicle primordium 2 of the guide thread 3 3a is housed inside the recess 11 in a posture in which the tip portion 3a is arranged so as to face the inner wall surface 11b.
  • the plurality of recesses 11 and the plurality of holding structures 5 are arranged side by side in the first direction orthogonal to the longitudinal direction of the guide thread 3 and parallel to the bottom surface 11a.
  • the holding structure 5 has a first wall portion 12 and a second wall portion 13.
  • the first wall portion 12 and the second wall portion 13 are respectively configured as wall portions extending upward from the bottom surface 11a, and the length of the guide thread 3 housed together with the regenerated hair follicle primordium 2 inside the recess 11. They are arranged so as to face each other so as to intersect in the direction.
  • the surface of the first wall portion 12 facing the second wall portion 13 is the stopper surface 12a, and the surface of the second wall portion 13 facing the first wall portion 12 is the stopper surface 13a.
  • the guide thread 3 can be arranged between the first wall portion 12 and the second wall portion 13 (between the stopper surface 12a and the stopper surface 13a). They are located apart.
  • the distance (interval) between the first wall portion 12 and the second wall portion 13 is the length obtained by adding the length of the guide thread 3 and the length of the regenerated hair follicle original group 2 (guide thread 3). Is longer than the length of the guide thread 3) when it penetrates the regenerated hair follicle primordia 2), and a guide extending straight between the first wall portion 12 and the second wall portion 13.
  • the thread 3 can be arranged so that one end in the longitudinal direction thereof faces the first wall portion 12 and the other end faces the second wall portion 13.
  • the first wall portion 12 and the second wall portion 13 can reduce the movement of the regenerated hair follicle primordium 2 arranged in the tip portion 3a of the guide thread 3 inside the recess 11 inside the recess 11. ..
  • the distance between the first wall portion 12 and the second wall portion 13 may be larger than the length of the guide thread 3.
  • the distance (interval) between the first wall portion 12 and the second wall portion 13 can be 3.2 to 11.0 mm. In the present embodiment, the distance (interval) is 7 mm.
  • the stopper surface 12a may be inclined inward so as to gradually approach the second wall portion 13 from the upper side to the lower side.
  • the stopper surface 13a may be inclined inward so as to gradually approach the first wall portion 12 from the upper side to the lower side.
  • the inclination angle ⁇ 1 or ⁇ 2 of the stopper surface 12a or the stopper surface 13a can be set to 2 to 50 °. In the present embodiment, the inclination angle ⁇ 1 or ⁇ 2 is 30 °. Further, the heights of the first wall portion 12 and the second wall portion 13 may be set to, for example, 2 to 10 mm.
  • the distance of the upper end between the first wall portion 12 and the second wall portion 13 is larger than that of the guide thread 3, and the distance of the lower end between the first wall portion 12 and the second wall portion 13 is the guide thread. It may be smaller than 3. In this case, for example, the distance of the upper end between the first wall portion 12 and the second wall portion 13 is set to 6 to 16 mm. Further, the distance of the lower end between the first wall portion 12 and the second wall portion 13 is set to 5 to 15 mm.
  • a plurality of support portions 14 and 15 are provided between the first wall portion 12 and the second wall portion 13.
  • the support portions 14 and 15 are configured as wall portions that extend upward from the bottom surface 11a of the recess 11 and are arranged along a first direction orthogonal to the longitudinal direction of the guide thread 3 housed in the recess 11.
  • the support portions 14 and 15 are arranged apart from each other in the longitudinal direction of the guide thread 3 and separated from the inner wall surfaces 11b, 11c, 11d and 11e of the recess 11. Further, the upper ends of the support portions 14 and 15 are located below the edge 11f of the opening of the recess 11.
  • a configuration may be configured in which three or more support portions are provided between the first wall portion 12 and the second wall portion 13.
  • the distance between the support portion 14 and the support portion 15, the distance between the support portion 15 and the first wall portion 12, and the distance between the support portion 14 and the second wall portion 13 become uniform. It may be arranged as follows. Alternatively, the guide thread 3 may be arranged so as to be easily grasped at the center. The distance between the center of the support portion 14 and the center of the support portion 15 in the longitudinal direction of the guide thread 3 can be 1 to 5 mm. In the present embodiment, the interval is 2 mm.
  • a groove 14a in which the guide thread 3 is arranged is provided at the upper end of the support portion 14.
  • the groove 14a has a V-shaped cross section, and the bottom portion thereof is in the longitudinal direction of the guide thread 3 (in the first direction) so as to go from one side to the other side of the first wall portion 12 and the second wall portion 13. It extends along the orthogonal second direction) and parallel to the bottom surface 11a.
  • the support portion 14 can support a portion of the groove 14a on the base end side opposite to the tip end portion 3a of the guide thread 3. Since the cross section of the groove 14a is V-shaped, there is an effect that the guide thread 3 can be easily inserted into the groove 14a and can be easily fixed at the bottom of the groove 14a.
  • the groove 14a is not limited to the V shape.
  • a groove 15a in which the guide thread 3 is arranged is provided at the upper end of the support portion 15.
  • the groove 15a has a V-shaped cross section, and the bottom portion thereof extends coaxially with the bottom portion of the groove 14a of the support portion 14.
  • the support portion 15 can support the tip portion 3a of the guide thread 3 in the groove 15a. Since the cross section of the groove 15a is V-shaped, there is an effect that the guide thread 3 can be easily inserted into the groove 15a and can be easily fixed at the bottom of the groove 15a.
  • the groove 15a is not limited to the V shape.
  • the bottom portions of the grooves 14a and 15a that support the guide thread 3 are located below the upper ends of the first wall portion 12 and the second wall portion 13, respectively.
  • the guide threads 3 arranged in the grooves 14a and 15a are restricted by the first wall portion 12 and the second wall portion 13 so that the movement in the longitudinal direction thereof is regulated by the first wall portion 12 and the second wall portion 12. It can be placed between the thirteenth.
  • the bottom portions of the grooves 14a and 15a that support the guide thread 3 are located below the height of half the height from the bottom surface 11a of the recess 11 to the edge 11f of the opening. As a result, the regenerated hair follicle primordium 2 stored in the recess 11 can be effectively immersed in the culture solution 4 stored in the recess 11.
  • the height of the support portions 14 and 15 from the bottom surface 11a is larger than the distance between the edge 11f of the opening of the recess 11 and the upper ends of the support portions 14 and 15.
  • the heights of the support portions 14 and 15 may be set to, for example, 1.5 to 10 mm.
  • the support portions 14 and 15 are provided in the plurality of recesses 11, respectively, and the plurality of guide threads 3 are supported by the support portions 14 and 15, respectively.
  • a plurality of regenerated hair follicle primordiums 2 arranged at the tip of the thread 3 can be held at positions separated upward from the bottom surface 11a.
  • the grooves 14a and 15a in which the guide threads 3 are arranged in the support portions 14 and 15 the movement of the guide threads 3 supported by the support portions 14 and 15 in the lateral direction (first direction) is restricted. Therefore, it is possible to reduce the lateral movement of the regenerated hair follicle primordium 2 arranged in the tip portion 3a of the guide thread 3 inside the recess 11.
  • the intermediate portion of the guide thread 3 is held in a state of floating from the bottom surface 11a between the two support portions 14 and 15. be able to.
  • the intermediate portion of the guide thread 3 can be easily moved between the support portion 14 and the support portion 15 by a tool such as tweezers. Can be picked up. Therefore, the work of taking out the regenerated hair follicle primordium 2 from the container 1 can be easily performed.
  • the portions of the support portions 14 and 15 above the bottom portions of the grooves 14a and 15a have a shape that becomes thinner toward the upper side, respectively. More specifically, in the portion of the support portion 14 above the bottom portion of the groove 14a, both the surface facing the side of the support portion 15 and the surface facing the side of the second wall portion 13 face upward from below. By inclining so as to approach each other, it becomes thinner upward. On the other hand, the portion of the support portion 15 above the bottom portion of the groove 15a is thinned upward because only the surface facing the side of the support portion 14 is inclined.
  • the inclination angles ⁇ 3 and ⁇ 4 of the portions above the bottom portions of the grooves 14a and 15a of the support portions 14 and 15 can be set to 30 to 88 °. In the present embodiment, their inclination angles ⁇ 3 and ⁇ 4 are 45 °.
  • the portions above the bottom portions of the grooves 14a and 15a of the support portions 14 and 15 have a shape that becomes thinner toward the upper side, respectively. Therefore, the container 1 is opened and the regenerated hair is regenerated from the inside of the recess 11.
  • a tool such as tweezers between the support portion 14 and the support portion 15, and the work of picking the guide thread 3 between the support portion 14 and the support portion 15 is further performed. Can be facilitated. Therefore, it is possible to more easily perform the work of picking the guide thread 3 with a tool such as tweezers and taking out the regenerated hair follicle primordium 2 from the container 1.
  • the regenerated hair follicle primordium 2 stored in the container 1 picks the base end portion of the guide thread 3 opposite to the tip portion 3a between the support portion 14 and the second wall portion 13 with a tool such as tweezers. It can also be taken out from the recess 11. Also in this case, since the surface of the support portion 14 facing the second wall portion 13 and the stopper surface 13a of the second wall portion 13 are inclined, between the support portion 14 and the support portion 15. Tools such as tweezers can be easily inserted. Therefore, the work of picking the base end portion of the guide thread 3 with a tool such as tweezers and taking out the regenerated hair follicle primordium 2 from the container 1 can be easily performed.
  • the lid portion 30 when the lid portion 30 has the presser foot convex portion 32 and the presser foot convex portion 33, the lid portion 30 can be easily inserted into the pedestal portion 16 and the pedestal portion 17 with which the presser convex portions 32 and 33 face each other. it can.
  • the length of the guide yarns 3 of the support portions 14 and 15 in the longitudinal direction is such that the support region supported by the support portions 14 and 15 of the guide yarn 3 is not supported by the support portions 14 and 15 of the guide yarn 3. Is also set to be small. As a result, the area that can be picked by a tool such as tweezers of the guide thread 3 stored in the container 1 is enlarged, and when the regenerated hair follicle primordium 2 is taken out from the inside of the recess 11, a tool such as tweezers is used. Therefore, the work of picking the guide thread 3 can be performed more easily.
  • a pedestal portion 16 is provided between the support portion 14 and the support portion 15 adjacent to the support portions 14 and 15. Further, a pedestal portion 17 is provided adjacent to the support portion 14 and the second wall portion 13.
  • the cradle portions 16 and 17 are configured as convex portions that project upward from the bottom surface 11a of the concave portion 11 and extend along the first direction, respectively.
  • the heights of the cradle portions 16 and 17 are lower than the bottom portions of the grooves 14a and 15a that support the guide threads 3 of the support portions 14 and 15, and the guide threads 3 and the receivers arranged in the grooves 14a and 15a are received.
  • a predetermined gap is provided between the upper surfaces of the bases 16 and 17.
  • the support portion 14 and the support portion 15 are provided in order to open the container 1 and take out the regenerated hair follicle primordium 2 from the inside of the recess 11.
  • a tool such as tweezers
  • the tip of the tool can be brought into contact with the base portion 16 to hold the guide thread 3 in a position where it can be easily picked. ..
  • a tool such as tweezers is inserted between the support portion 14 and the second wall portion 13, and the tool is used.
  • the tip of the tool can be brought into contact with the base portion 17 to hold the guide thread 3 in a position where it can be easily picked. Therefore, it is possible to more easily perform the work of picking the guide thread 3 with a tool such as tweezers and taking out the regenerated hair follicle primordium 2 from the container 1.
  • the length (width) of the pedestal portion 16 in the first direction is longer than the length (width) of the pedestal portion 17. More specifically, the length of the pedestal portion 17 in the first direction is the same as the length of the support portions 14, 15 and the second wall portion 13 in the first direction, whereas the length of the pedestal portion 16 is the same.
  • the length in one direction is longer than the length of the support portions 14, 15 and the second wall portion 13 in the first direction.
  • the holding structure 5 has a pair of side walls 18 and 19 that are integrally connected to the side portion of the support portion 15 and project from the inner wall surfaces 11c and 11d toward the inside of the recess 11.
  • the pair of side walls 18 and 19 are configured as wall portions extending upward from the bottom surface 11a of the recess 11 and parallel to the longitudinal direction of the guide thread 3 housed in the recess 11.
  • the pair of side walls 18 and 19 may be the inner wall surfaces 11c and 11d of the recess 11.
  • a storage portion 20 for storing the regenerated hair follicle primordium 2 may be provided inside the recess 11.
  • the storage portion 20 is partitioned inside the recess 11 as a region surrounded by the first wall portion 12, the support portion 15, and the pair of side walls 18 and 19.
  • the upper ends of the first wall portion 12, the support portion 15, and the pair of side walls 18 and 19 are located below the edge 11f of the opening of the recess 11, respectively, and the culture solution 4 is the culture solution 4.
  • a predetermined amount (for example, 300 ⁇ L) is stored inside the recess 11 so that the liquid level is located above the upper ends of the first wall portion 12, the support portion 15, and the pair of side walls 18 and 19.
  • the culture solution 4 stored in the recess 11 is arranged in the first wall portion 12, the support portion 15, and the storage portion 20 inside the pair of side walls 18 and 19 inside the recess 11, and inside the recess 11. It is also arranged in other areas other than the storage unit 20.
  • the culture solution 4 flows in and out of the storage portion 20 and other regions above the upper ends of the first wall portion 12, the support portion 15, and the pair of side walls 18 and 19. It is continuous as possible. Therefore, since the storage unit 20 is in a state in which a predetermined amount of the culture solution 4 is stored, the regenerated hair follicle primordium 2 is maintained in a state of being immersed in the culture solution 4.
  • the regenerated hair follicle primordium 2 arranged in the tip portion 3a of the guide thread 3 is held inside the storage portion 20, and the side walls 18, 19 It is possible to reduce the contact with the regenerated hair follicle primordium 2 and reduce the damage to the regenerated hair follicle primordium 2.
  • the distance from the bottom surface 20a of the storage portion 20 to the bottom portion of the groove 15a of the support portion 15 is along the direction perpendicular to the longitudinal direction of the guide thread 3 of the regenerated hair follicle primordium 2 arranged in the storage portion 20. It is larger than half the diameter (half the maximum width in the direction perpendicular to the longitudinal direction of the guide thread 3).
  • the height may be set to, for example, 0.5 to 2 mm.
  • the nutrient of the culture solution 4 is distributed to the regenerated hair follicle primordium 2 arranged in the storage portion 20. Therefore, it is possible to reduce the deterioration of the quality of the regenerated hair follicle primordium 2.
  • the lid portion 30 includes an elongated rectangular plate-shaped lid main body 31 corresponding to the outer shape of the container main body portion 10.
  • the lid body 31 covers all the openings of the plurality of recesses 11 and the container body portion 10 is closed.
  • the lid main body 31 may be liquid-tightly fixed to the entire circumference of the edge 11f of the opening of each recess 11 by means such as welding or adhesion to seal the opening of each recess 11.
  • the lid portion 30 may further include at least one pressing convex portion arranged on the lid main body 31.
  • the lid main body 31 may be provided with at least one pressing convex portion extending downward from the lower surface of the lid main body 31.
  • a pair of pressing protrusions 32, 33 are provided on the lower surface of the lid main body 31.
  • the pressing protrusions 32 and 33 are arranged in parallel with each other at intervals in the longitudinal direction of the guide thread 3.
  • the pressing portion convex portion 32 is located in the region between the supporting portion 14 and the supporting portion 15, and the pressing portion convex portion 33 includes the supporting portion 14 and the second wall portion 13. It is designed to be located in the area between.
  • the lid main body 31 may have a configuration in which only one pressing convex portion is provided. Further, the lid main body 31 may be configured to be provided with three or more pressing protrusions.
  • the length of the presser foot convex portion 32 along the longitudinal direction of the guide thread 3 may be smaller than the length of the pedestal portion 16 along the longitudinal direction of the guide thread 3, thereby supporting the presser foot convex portion 32. It can be arranged in the area between the 14 and the support portion 15.
  • the length of the presser foot convex portion 33 along the longitudinal direction of the guide thread 3 may be smaller than the length of the pedestal portion 17 along the longitudinal direction of the guide thread 3, thereby causing the presser foot convex portion 33 to be formed. It can be arranged in the area between the support portion 14 and the second wall portion 13.
  • the length of the presser foot convex portion 32 along the longitudinal direction of the guide thread 3 may be smaller than the length of the guide thread 3 between the support portion 14 and the support portion 15 along the longitudinal direction. , The pressing convex portion 32 can be easily inserted between the supporting portion 14 and the supporting portion 15.
  • the length of the presser foot convex portion 33 along the longitudinal direction of the guide thread 3 may be smaller than the length of the support portions 14 and 15 along the longitudinal direction of the guide thread 3, whereby the support portion 14 may be formed. The pressing convex portion 33 can be easily inserted between the and the second wall portion 13.
  • the lower ends of the pressing protrusions 32 and 33 can be configured to be located below the upper ends of the first wall portion 12 and the second wall portion 13, respectively.
  • the guide thread 3 is positioned between the first wall portion 12 and the second wall portion 13 in a state where the regenerated hair follicle primordium 2 is stored in the storage portion 20 inside the recess 11. It can be positioned in the vertical direction.
  • the lower ends of the pressing convex portions 32 and 33 can be configured to be located above the bottom portions of the grooves 14a and 15a that support the guide threads 3 of the supporting portions 14 and 15, respectively.
  • the movement of the guide thread 3 arranged in the grooves 14a and 15a of the support portions 14 and 15 is restricted by the convex portions 32 and 33, and the guide thread 3 is positioned in the vertical direction. Can be done.
  • the storage portion 20 is visible from the outside of the recess 11.
  • the container body 10 and the lid 30 are made of a transparent material as a whole, and the storage portion 20 can be visually recognized from an arbitrary direction outside the recess 11.
  • the regenerated hair follicle primordium 2 stored in the recess 11 can be observed from the outside through the flat surface 22a of the container 1 and its state can be confirmed. This makes it possible to appropriately check and manage the state of the regenerated hair follicle primordium 2 stored in the container 1.
  • a recess 21 is provided in the container body 10 so that the regenerated hair follicle primordium 2 stored in the storage portion 20 can be observed more effectively.
  • the recessed portion 21 is provided at a position corresponding to the storage portion 20 of the container main body portion 10, and is directed upward from the outer bottom surface 11 g of the recessed portion 11 so as to open to the outside of the recessed portion 11. It is dented.
  • the bottom surface 20a of the storage 20 is located above the bottom 11a of the recess 11, and the storage 20 is vertically oriented by that amount. It is getting narrower.
  • the bottom wall of the container body 10 in the portion where the recess 21 is provided is a flat window wall 22, and the flat surface 22a facing the outside of the window wall 22 is located above the outer bottom surface 11g of the recess 11. There is.
  • the vertical distance from the flat surface 22a to the bottom surface 20a of the storage unit 20 is smaller than the vertical distance from the outer bottom surface 11g of the recess 11 to the bottom surface 20a of the storage unit 20.
  • the distance between the flat surface 22a of the container main body portion 10 and the bottom surface 20a of the storage portion 20 of the recess 11 is set to the recessed portion. It can be made smaller than the case where 21 is not provided. Therefore, the regenerated hair follicle primordium 2 stored in the storage unit 20 can be observed from a closer position through the window wall 22 from the plane 22a side. The observation can be performed using, for example, a microscope.
  • the container body 10 and the lid 30 are made transparent as a whole, but if at least the window wall 22 of the container body 10 is transparent, the other parts may be opaque. It may be transparent.
  • FIG. 5 is a cross-sectional view of the container 100 according to the second embodiment of the present disclosure
  • FIG. 6 is a perspective view of the container main body 10 shown in FIG. 5
  • FIG. 7 is a perspective view of the container main body 10 shown in FIG. It is a plan view.
  • the members corresponding to the above-mentioned members are designated by the same reference numerals, and the description thereof will be omitted again.
  • the container 100 according to the second embodiment of the present disclosure shown in FIGS. 5 to 7 has a lid portion having a lid main body 31 provided with a pair of pressing convex portions 32, 33 as a member for closing the container main body portion 10.
  • a lid 40 formed in a thin and flexible sheet shape made of a synthetic resin material, an aluminum alloy, or the like is used.
  • the lid 40 is liquid-tightly fixed to the entire circumference of the edge 11f of the opening of each recess 11 by means of welding, adhesion, or the like, thereby covering and sealing all the openings of each recess 11.
  • the upper ends of the first wall portion 12 and the second wall portion 13 provided in the container main body portion 10 are located at the same height as the edge 11f of the opening of the recess 11, and the lid 40 is the first wall portion. It is in contact with the upper ends of 12 and the second wall portion 13.
  • the upper ends of the pair of support portions 14 and 15 provided on the container body 10 are also located at the same height as the edge 11f of the opening of the recess 11, and the lid 40 is located on the upper ends of the pair of support portions 14 and 15. Are also in contact.
  • the lid 40 By making the lid 40 in contact with the upper ends of the pair of support portions 14 and 15, the openings of the grooves 14a and 15a can be closed by the lid 40. Therefore, the guide thread 3 can be held inside the grooves 14a and 15a without providing the lid 40 with the portions corresponding to the pressing protrusions 32 and 33.
  • the guide thread 3 is provided along the grooves 14a and 15a of the pair of supporting portions 14 and 15 and the upper ends of the grooves 14a and 15a. May move to the side.
  • the culture solution 4 fills the entire inside of the recess 11, and the liquid level thereof is located at the same height as the edge 11f of the opening of the recess 11. Therefore, even if the guide thread 3 moves to the upper end side of the grooves 14a and 15a, the regenerated hair follicle primordium 2 is maintained in a state of being immersed in the culture solution 4.
  • the guide thread 3 moves to the upper end side of the grooves 14a and 15a.
  • the guide thread 3 can be positioned in the longitudinal direction by the first wall portion 12 and the second wall portion 13.
  • the grooves 14a and 15a of the support portions 14 and 15 have a depth directed downward from the upper ends of the support portions 14 and 15 along a direction perpendicular to the longitudinal direction of the guide thread 3 of the regenerated hair follicle primordium 2, respectively. It can be configured to be deeper than the diameter (half of the maximum width in the direction perpendicular to the longitudinal direction of the guide thread 3). Thereby, it is possible to reduce the contact of the regenerated hair follicle primordium 2 with the lid 40 in a state where the guide thread 3 is supported by the grooves 14a and 15a.
  • the upper ends of the first wall portion 12 and the second wall portion 13 and the upper ends of the pair of support portions 14 and 15 may be fixed to the lid 40 by means such as welding and adhesion, respectively.
  • FIG. 8 is a perspective view of the container 200 according to the third embodiment of the present disclosure, in a state where the lid portion 30 is removed from the container main body portion 10.
  • the members corresponding to the above-mentioned members are designated by the same reference numerals, and the description thereof will be omitted again.
  • the container 1 shown in FIG. 1 has a configuration in which a plurality of (10) recesses 11 are provided in the container main body 10 side by side in the first direction.
  • the container main body 10 is provided with a plurality of (10) recesses 11 arranged in the first direction, and a row of recesses 11 composed of the plurality of recesses 11 is arranged in the second direction to form a plurality of rows (5).
  • Column It is also possible to provide a configuration. That is, in the container 200 according to the third embodiment, the container main body 10 has a configuration in which a plurality of (50) recesses 11 and a holding structure 5 are arranged in a grid pattern.
  • the lid portion 30 As the lid portion 30, a larger one that matches the outer shape of the container main body portion 10 is used, and all the recesses 11 are closed by the lid portion 30.
  • the container 200 according to the third embodiment having such a configuration since the regenerated hair follicle primordium 2 can be stored together with the guide thread 3 in each recess 11, more hair follicle regenerating primordium 2 can be stored. Can be stored and transported while reducing its quality deterioration.
  • FIG. 9 is a perspective view of the container 300 according to the fourth embodiment of the present disclosure, in a state where the lid portion 30 is removed from the container main body portion 10.
  • the members corresponding to the above-mentioned members are designated by the same reference numerals, and the description thereof will be omitted again.
  • a plurality of (10) recesses 11 are provided in the container body 10 in the first direction, and a holding structure 5 is provided in each recess 11, and the regenerated hair follicle primordium 2 and the guide thread 3 are provided.
  • a plurality of holding structures 5 may be provided side by side in one direction, and a plurality of regenerated hair follicle primordiums 2 and guide threads 3 may be arranged by holding the holding structures 5 in each holding structure 5. That is, the container 300 according to the fourth embodiment has a configuration in which the partition wall that isolates the adjacent recesses 11 is removed from the container 1 according to the first embodiment.
  • the container 300 according to the fourth embodiment having such a configuration all the regenerated hair follicle primordiums 2 and guides held in the respective holding structures 5 are filled in the culture solution 4 filled in one recess 11.
  • the thread 3 is immersed. Therefore, the work of filling the recess 11 with the culture solution 4 can be facilitated, and the culture solution 4 is discharged from the recess 11 and regenerated before the regenerated hair follicle primordium 2 is transplanted to the alopecia site of the patient.
  • the work of filling the recess 11 with a liquid for cleaning the hair follicle primordium 2 can be easily performed.
  • FIG. 10 is a perspective view of the container 400 according to the fifth embodiment of the present disclosure in a state where the lid portion 30 is removed from the container main body portion 10, and
  • FIG. 11 is a perspective view of the sub-container 10B shown in FIG. ..
  • the members corresponding to the above-mentioned members are designated by the same reference numerals, and the description thereof will be omitted again.
  • the container main body 10 has a main container 10A and a plurality of sub-containers 10B.
  • the plurality of sub-containers 10B can be attached to and detached from the main container 10A, respectively.
  • the main container 10A has one main recess 11A constituting the recess 11, and a plurality of (10) sub-containers 10B are arranged in the first direction inside the main recess 11A. It is arranged.
  • Each of the sub-containers 10B is arranged at a distance from the inner peripheral wall of the main recess 11A, and is slightly spaced between adjacent sub-containers 10B.
  • each sub-container 10B is a sub-recess 11B constituting the recess 11 together with the main recess 11A.
  • a holding structure 5 similar to that in the container 1 of the first embodiment is provided, and is held by these holding structures 5 to be held in each sub-container.
  • one guide thread 3 and one regenerated hair follicle primordium 2 arranged at the tip of the guide thread 3 are arranged.
  • the sub-recess 11B corresponds to the recess 11 in the container 1 of the first embodiment.
  • the opening of the main recess 11A and the opening of the sub recess 11B have the same height.
  • Each sub-container 10B is provided with a through hole 50 penetrating the sub-container 10B.
  • each of the sub-containers 10B is provided with two through holes 50 penetrating the peripheral wall portion at the inner wall surface 11c and 11d. These through holes 50 are located below the openings of the main recess 11A and the sub recess 11B, and are open to the inside of the main recess 11A and the inside of the sub recess 11B. Therefore, the main recess 11A and the sub recess 11B are communicated with each other by the through hole 50.
  • the culture solution 4 is filled in the main recess 11A and the sub-recess 11B of each sub-container 10B. As described above, since the main recess 11A and the sub-recess 11B are communicated with each other by the through hole 50, the sub-recess 11B of each sub-container 10B communicates with each other via the main recess 11A.
  • the container 400 according to the fifth embodiment having such a configuration, it is possible to facilitate the work of filling the sub-recess 11B of each sub-container 10B with the culture solution 4, and the regenerated hair follicle primordia.
  • the culture solution 4 is discharged from the sub-recess 11B of each sub-container 10B, and the liquid for washing the regenerated hair follicle primordia 2 is sub-container 10B.
  • the work of filling the recess 11B can be easily performed.
  • the regenerated hair follicle primordium 2 is attached to the alopecia site of the patient.
  • the sub-containers 10B can be taken out one by one from the main container 10A and the work can be performed. This makes it possible to easily perform the work of transplanting the regenerated hair follicle primordium 2 to the alopecia site of the patient.
  • the holding structure 5 includes a plurality of support portions 14, 15 for supporting the plurality of guide threads 3, the structure or shape thereof may be different.
  • the holding structure 5 may not include the first wall portion 12, the second wall portion 13, and the cradle portions 16 and 17.
  • the holding structure 5 may have a configuration in which only one support portion is provided in each recess 11.
  • the first direction in which the holding structures 5 are arranged side by side is set to the direction orthogonal to the longitudinal direction of the guide thread 3, but the longitudinal direction of the guide thread 3 is defined as the direction orthogonal to the longitudinal direction of the guide thread 3.
  • the directions are not limited to orthogonal directions.
  • a through hole may be provided in the partition wall that isolates the adjacent recesses 11, and the adjacent recesses 11 may be communicated with each other through the through holes. ..
  • the containers 300 and 400 shown in FIGS. 9 and 10 also have a configuration in which a plurality of recesses 11 or sub-containers 10B (sub-containers 11B) arranged in a grid pattern are provided as in the container 200 shown in FIG. You can also do it.
  • the sub-container 10B may be integrally configured with the main container 10A so that the sub-container 10B cannot be attached to or detached from the main container 10A.
  • Container 2 Regenerated hair wrap original 3 Guide thread 4 Culture solution 5 Retention structure 10
  • Container body 10A Main container 10B Sub container 11 Recess 11A Main recess 11B Sub recess 11a Bottom 11b Inner wall surface 11c Inner wall surface 11d Inner wall surface 11e Inner wall surface 11f Edge of opening 12 1st wall part 12a Stopper surface 13 2nd wall part 13a Stopper surface 14 Support part 14a Groove 15 Support part 15a Groove 16 Receiving part 17 Receiving part 18 Side wall 19 Side wall 20 Storage part 20a Bottom surface 21 Recessed part 22 Window 22a Flat 30 Lid 31 Lid body 32 Convex 33 Convex 40 Lid 50 Through hole 100 Container 200 Container 300 Container 400 Container 400 Container

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cosmetics (AREA)

Abstract

L'invention concerne un récipient (1, 100, 200, 300, 400) caractérisé en ce qu'il comporte : des évidements (11) ouverts vers le haut ; une pluralité de fils de guidage (3) disposés à l'intérieur des évidements (11) ; une pluralité de germes de follicules pileux régénératifs (2) disposés au niveau de parties d'extrémité (3a) de la pluralité de fils de guidage (3) ; et une pluralité de parties de support (14, 15) pour supporter la pluralité de fils de guidage (3).
PCT/JP2020/036731 2019-09-30 2020-09-28 Récipient Ceased WO2021065831A1 (fr)

Priority Applications (1)

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JP2021551269A JP7372621B2 (ja) 2019-09-30 2020-09-28 容器

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JP2019180898 2019-09-30
JP2019-180898 2019-09-30

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WO2021065831A1 true WO2021065831A1 (fr) 2021-04-08

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012108069A1 (fr) * 2011-02-09 2012-08-16 株式会社オーガンテクノロジーズ Procédé pour produire une ébauche d'organe régénératrice pourvue d'un guide pour transplantation, composition contenant une ébauche d'organe régénératrice pourvue d'un guide pour transplantation produite par celui-ci, et procédé pour transplanter une ébauche d'organe régénératrice pourvue d'un guide pour transplantation
JP2019135947A (ja) * 2018-02-07 2019-08-22 国立大学法人横浜国立大学 毛包上皮幹細胞の培養方法及び培養キット

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012108069A1 (fr) * 2011-02-09 2012-08-16 株式会社オーガンテクノロジーズ Procédé pour produire une ébauche d'organe régénératrice pourvue d'un guide pour transplantation, composition contenant une ébauche d'organe régénératrice pourvue d'un guide pour transplantation produite par celui-ci, et procédé pour transplanter une ébauche d'organe régénératrice pourvue d'un guide pour transplantation
JP2019135947A (ja) * 2018-02-07 2019-08-22 国立大学法人横浜国立大学 毛包上皮幹細胞の培養方法及び培養キット

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OGAWA, MIHO ET AL.: "Future regenerative medicine that regenerates hair from ''seed", CHEMISTRY TODAY, 1 April 2019 (2019-04-01), pages 26 - 29 *

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JP7372621B2 (ja) 2023-11-01

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