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WO2020201343A1 - Extrait d'alteromonadaceae et son utilisation cosmétique - Google Patents

Extrait d'alteromonadaceae et son utilisation cosmétique Download PDF

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Publication number
WO2020201343A1
WO2020201343A1 PCT/EP2020/059234 EP2020059234W WO2020201343A1 WO 2020201343 A1 WO2020201343 A1 WO 2020201343A1 EP 2020059234 W EP2020059234 W EP 2020059234W WO 2020201343 A1 WO2020201343 A1 WO 2020201343A1
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WO
WIPO (PCT)
Prior art keywords
extract
composition
skin
alteromonadaceae
exopolysaccharide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2020/059234
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English (en)
Inventor
Bethsabée COUTAZ
Aïna Laure QUEIROZ
Jean Georges GUEZENNEC
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ID Bio
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ID Bio
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Filing date
Publication date
Application filed by ID Bio filed Critical ID Bio
Publication of WO2020201343A1 publication Critical patent/WO2020201343A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Definitions

  • TITLE Alteromonadaceae extract and its cosmetic use
  • the present invention relates to a particular extract of Alteromonadaceae and a method of cosmetic treatment of the skin using this extract.
  • the present invention relates to an exopolysaccharide included in this extract.
  • Exopolysaccharides are high molecular weight polymers composed of oses and secreted by a microorganism in its immediate environment. Due to the great diversity of composition, exopolysaccharides have found many applications in various industries.
  • bacterial exopolysaccharides are advantageous because their production and quality do not depend on climatic, ecological and political risks unlike the equivalent extracted from algae or plants.
  • these polymers offer a greater degree of structural regularity and may be extracted and purified more easily.
  • the inventors discovered a new exopolysaccharide of a particularly large size, and very rich in uronic acid.
  • the use of this new exopolysaccharide makes it possible to improve the luminosity of the skin, and to standardize the complexion and the texture of the skin, thereby improving the radiance of the complexion, while promoting hydration and generating a feeling of well-being.
  • this exopolysaccharide due to its high molecular weight, gives sensory properties to the cosmetic composition and reinforces this feeling of well-being.
  • the effective concentration is lower than those conventionally described for other exopolysaccharides, which is of economic interest to the formulator.
  • This exopolysaccharide may, in particular, be obtained from Alteromonadaceae.
  • the present invention therefore relates to an Alteromonadaceae extract comprising an exopolysaccharide with a molecular weight between 3.10 6 and 5.10 6 Da which comprises the following amounts: glucose, galactose, glucuronic acid and galacturonic acid.
  • the object of the invention is also a cosmetic composition comprising an extract according to the invention.
  • the object of the invention is a cosmetic use and a method of cosmetic treatment of the skin using an extract according to the invention, or a composition according to the invention.
  • the Alteromonadaceae extract according to the invention comprises an exopolysaccharide with a molecular weight of between 3.10 6 and 5.10 6 Da, preferably between 3.5 and 4.5.10 6 Da.
  • the molecular weight may be determined by techniques well known to those skilled in the art and, in particular, by High Performance Steric Exclusion Chromatography (HPSEC).
  • the exopolysaccharide present in the extract according to the invention comprises the following forms: glucose, galactose, glucuronic acid and galacturonic acid.
  • the exopolysaccharide included in the extract comprises, in decreasing masses, galactose, then glucuronic acid, then galacturonic acid in an amount of the same order as glucose.
  • Uronic acids are chemical compounds obtained by oxidation of the last carbon of simple oses.
  • the exopolysaccharide present in the extract comprises more than 20% by weight of uronic acid, preferably more than 30% and more particularly more than 35% of uronic acid.
  • the exopolysaccharide comprises between 35 and 45% by weight of uronic acid.
  • exopolysaccharide present in the extract of the invention may carry substituents, in particular lactate or acetate substituents.
  • the exopolysaccharide present in the extract according to the invention does not include any substituents other than the lactate and acetate substituents.
  • the exopolysaccharide according to the invention does not comprise a sulfate group.
  • the osidic composition of the exopolysaccharide present in the extract of the invention was determined by gas chromatography.
  • the exopolysaccharide present in the extract according to the invention is preferably very hygroscopic.
  • the extract according to the invention comprises between 0.1 and 10% by weight of this exopolysaccharide.
  • the extract according to the invention has a polydispersity index of less than 2 and more particularly between 1 and 1 .5.
  • the polydispersity index was determined by steric exclusion chromatography.
  • the extract according to the invention comes from Alteromonas sp.
  • the extract is obtained from the microorganism of Alteromonas sp. deposited according to the Budapest Treaty, on November 6, 2018, with the National Collection of Cultures of Microorganisms ((CNCM), Paris, France) under the number CNCM I-5375). This microorganism normally associated with sea anemone was collected in the lagoon of Moorea.
  • the invention also relates to an Alteromonadaceae extract capable of being obtained by a production process comprising the following stages:
  • step (ii) centrifugation of the culture obtained in step (i) between 15,000 and 20,000 g.
  • step (v) dilution of the retentate obtained in step (iii) in water
  • the process may also comprise a step (viii) of concentration and lyophilization or atomization.
  • seawater is meant salt water comprising 5 to 40 g of salts per liter of water and having a pH of between 7.5 and 8.4.
  • the seawater used in the process is seawater reconstituted by diluting synthetic salt.
  • the seawater used in the process is seawater reconstituted by dilution of "Instant Ocean ® " synthetic salt at a concentration of 12 g/L.
  • stirring is meant a stirring speed between 50 to 300 rpm, preferably between 100 and 200 rpm.
  • ultrafiltration is meant filtration through a filter having pores with a diameter between 1 and 20 nm, preferably between 5 and 15 nm.
  • the sterilizing filtration, atomization and lyophilization steps are steps well known to those skilled in the art.
  • the extract according to the invention is stored under vacuum, or dry and cold, preferably between 0 and 25°C, more preferably between 2 and 8°C, and in particular at about 4°C.
  • the object of the invention is also a composition, in particular a cosmetic composition, comprising an extract according to the invention.
  • the invention also relates to a composition, in particular a cosmetic composition, comprising an extract capable of being obtained by the production process according to the invention.
  • the composition according to the invention comprises between 0.001 % and 5% by weight of exopolysaccharide as defined above relative to the weight of the composition.
  • the composition according to the invention comprises between 0.002 and 0.1 % by weight of exopolysaccharide as defined above relative to the weight of the composition, more particularly between 0.004 and 0.01 % by weight relative to the weight of the composition. In fact, even at very low concentrations, the exopolysaccharide retain their effectiveness.
  • the big size of the exopolysaccharide present in the composition gives it interesting sensorial properties. In particular, a feeling of softness to the touch.
  • the invention also relates to a non-therapeutic cosmetic use of an extract according to the invention or of a composition according to the invention for moisturizing the skin and/or improving the radiance of the complexion.
  • concentration of said exopolysaccharide in the composition or in the extract is between 0.001 % and 5% by weight relative to the weight of the composition or of the extract respectively.
  • Improving the radiance of the complexion is understood to mean an increase in the luminosity of the skin and/or an improvement in the texture of the skin and/or homogenization of the color of the skin.
  • skin is meant the human skin and/or the human scalp.
  • skin is meant the skin of the body, in particular of the legs, arms, hands, neck and face.
  • skin means the skin of the face.
  • the invention also relates to a non-therapeutic cosmetic use of an extract according to the invention or of a composition according to the invention, to promote a feeling of well being.
  • composition according to the invention or the extract according to the invention may be used in a subject having sensitive skin and, in particular, dry and/or aged skin.
  • the composition according to the invention or the extract according to the invention may be used in a subject having dull skin and/or with an unsatisfactory texture and/or an irregular complexion and/or colored spots.
  • composition according to the invention or the extract according to the invention may be used for a subject having a dry scalp.
  • composition used according to the invention or the extract used according to the invention is intended for topical application.
  • compositions used according to the invention preferably contain a physiologically acceptable medium, i.e. compatible with the skin.
  • This physiologically acceptable medium may consist, in particular, of water and, optionally, a physiologically acceptable organic solvent such as, for example, propanediol.
  • Said composition may, in particular, be in the form of a cream or lotion.
  • the composition may be for regular application and, in particular, for daily or bi-daily application.
  • compositions according to the invention are those generally used in the fields considered.
  • these compositions are prepared according to the usual methods.
  • the composition is used according to the invention as an active agent in an effective amount, i.e. in an amount allowing it to have the desired effect.
  • the composition may also comprise at least one adjuvant, in particular adjuvants usually used in the cosmetic field, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic additives, preservatives, antioxidants, solvents, perfumes, fillers, filters, odor absorbers and coloring matter.
  • adjuvants usually used in the cosmetic field such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic additives, preservatives, antioxidants, solvents, perfumes, fillers, filters, odor absorbers and coloring matter.
  • the amounts of these various adjuvants are those conventionally used in the cosmetic field, and may, for example, vary from approximately 0.01 % to 10% of the total weight of the composition.
  • These adjuvants depending on their nature, may be introduced into the fatty phase, into the aqueous phase, and/or into the lipid spherules.
  • the invention also relates to a cosmetic method for moisturizing and/or improving the radiance of the skin complexion comprising the step of topical application of an effective amount of a composition of the invention or of an extract according to the invention.
  • the invention relates to a cosmetic process for promoting a feeling of well-being comprising the step of topical application of an effective amount of a composition of the invention, or of an extract according to the invention.
  • Figure 1 represents the chromatogram obtained after High Performance Steric Exclusion Chromatography (HPSEC), of the Alteromonadaceae extract obtained in example 1 .
  • HPSEC High Performance Steric Exclusion Chromatography
  • Detectors LS: Light scattering
  • UV Ultraviolet absorption spectrometer
  • DRI Differential refractometer.
  • Figure 2 represents the chromatogram obtained after gas chromatography (GC) of the Alteromonadaceae extract obtained in Example 1 .
  • FIG 3 represents a graph representing the evolution between the start of the test (at day 0, named ‘DO’ or‘JO) and the end of the test (at day 28, named‘D28’ or‘J28’) of the brightness, the color homogeneity, and the consistency of skin texture. These three parameters are used to assess the radiance of the complexion. Two compositions were tested, a so-called placebo and one comprising the extract according to the invention (abbreviated EPS) (see example 4). * : statistically significant compared to DO (p ⁇ 0.05).
  • FIG 4 represents a graph showing the evolution between the start of the test (DO) and the end of the test (D28) of skin hydration.
  • DO start of the test
  • D28 end of the test
  • EPS extract according to the invention
  • FIG 5 represents a graph showing the evolution between the start of the test (DO) and the end of the test (D28) of the frequency of the voice.
  • DO start of the test
  • D28 end of the test
  • EPS extract according to the invention
  • FIG 6 Figure 6 represents a graph showing the evolution between the start of the test (DO) and the end of the test (D28) of the volume of the voice.
  • DO start of the test
  • D28 end of the test
  • EPS extract according to the invention
  • Figure 7 represents the evolution between the start of the test (DO) and the end of the test (D28) of the words used by the volunteers to answer the question "How would you describe yourself physically?" (See example 5).
  • the ordinate axis represents the number of statistical occurrences.
  • Figure 8 represents the evolution between the start of the trial (DO) and the end of the trial (D28) of the words used by the volunteers to answer the question "What would be the first impression of someone who meet you for the first time?” (see example 5).
  • the ordinate axis represents the number of statistical occurrences.
  • Example 1 The extract obtained in Example 1 was analyzed by High Performance Steric Exclusion Chromatography (HPSEC), see Figure 1 . This figure shows that the extract obtained is of high purity. Analysis of the results made it possible to determine the polydispersity index and the weight of the molecule detected. The polydispersity index is 1 .4, which confirms the purity of the extract. The weight of the detected molecule is 4.16.10 6 Da.
  • HPSEC High Performance Steric Exclusion Chromatography
  • EPS polysaccharide
  • the inventors have also determined by Nuclear Magnetic Resonance (NMR) that the EPS comprises lactate and acetate substituents. Finally, no sulfate group was detected by Fourier transform infrared spectroscopy (FTIR).
  • NMR Nuclear Magnetic Resonance
  • FTIR Fourier transform infrared spectroscopy
  • the interaction profile of the extract was obtained by testing the capacity of this extract to inhibit the interactions between a lectin and the binding ose. 20 lectin/ose pairs (see Table 1 ) were tested at 3 different concentrations.
  • the exopolysaccharide from the extract of Example 1 had an effect on the following lectins ConA LCH, DC-Sign, BC2L-A, AIA, PNA, PA-IL, GSL-II, WGA, HPA and COR-M .
  • This shows that the exopolysaccharide comprises glucose, galactose, glucuronic acid and galacturonic acid residues and bioavailable rhamnose-like units.
  • These results confirm the glycosidic sequence deciphered in GC, and reveal a new motif, the rhamnose-like motif.
  • DC-Sign is involved in chronic skin inflammations.
  • COR-M indicates the presence of a bioavailable rhamnose-like motif and indicates that the exopolysaccharide could promote dermo-epidermal regeneration by activating the extracellular matrix and by promoting the production of gene mediating inflammation.
  • the extract provides a significantly more satisfactory result than the placebo.
  • the hydration of the skin is improved in the presence of the extract compared to the placebo solution.
  • the extract therefore hydrates the skin.
  • the extract also makes it possible to increase the luminosity of the skin, to improve the texture of the skin, and to homogenize the color of the skin, thus improving the radiance of the complexion.
  • the Emospin test was used to assess the emotional impact of using the extract.
  • the prosody of the volunteers in Example 4 was studied before the test and at the end.
  • the frequency (measured in Hertz (Hz)) and the volume (measured in decibels (dB)) of the voice of the volunteers in Example 4 were evaluated (see Figures 5 and 6).
  • the inventors observed that the frequency and the volume of the voice of the volunteers who used the extract decreased significantly compared to the volunteers who used the placebo. This decrease is a sign of relaxation and therefore improvement in the person's feeling of well-being.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Engineering & Computer Science (AREA)
  • Dermatology (AREA)
  • Cosmetics (AREA)

Abstract

La présente invention concerne un extrait particulier d'Alteromonadaceae et un procédé de traitement cosmétique de la peau à l'aide de cet extrait. En particulier, la présente invention concerne un exopolysaccharide.
PCT/EP2020/059234 2019-04-01 2020-04-01 Extrait d'alteromonadaceae et son utilisation cosmétique Ceased WO2020201343A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FRFR1903473 2019-04-01
FR1903473A FR3094225A1 (fr) 2019-04-01 2019-04-01 Extrait d’Alteromonadaceae et son utilisation cosmétique

Publications (1)

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WO2020201343A1 true WO2020201343A1 (fr) 2020-10-08

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004103265A2 (fr) * 2003-05-26 2004-12-02 Enprani Co., Ltd. Composition cosmetique de blanchiment et antioxydante renfermant du resveratrol et procede de preparation de celle-ci
FR2871476A1 (fr) * 2004-06-14 2005-12-16 Ifremer Derives depolymerises sulfates d'exopolysaccharides (eps) provenant de bacteries marines mesophiles, leur procede de preparation et leurs utilisations en regeneration tissulaire
WO2011026039A1 (fr) * 2009-08-28 2011-03-03 Mary Kay Inc. Compositions de soin de la peau
WO2012001164A1 (fr) * 2010-07-02 2012-01-05 Ifremer (Institut Français De Recherche Pour L'exploitation De La Mer) Exopolysaccharides pour la prévention et la lutte contre la formation de biofilms
WO2017015596A1 (fr) * 2015-07-23 2017-01-26 Mary Kay Inc. Formulations topiques pour la peau
WO2017019265A1 (fr) * 2015-07-30 2017-02-02 Truth Aesthetics LLC Composition topique de soin de jour pour la peau

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004103265A2 (fr) * 2003-05-26 2004-12-02 Enprani Co., Ltd. Composition cosmetique de blanchiment et antioxydante renfermant du resveratrol et procede de preparation de celle-ci
FR2871476A1 (fr) * 2004-06-14 2005-12-16 Ifremer Derives depolymerises sulfates d'exopolysaccharides (eps) provenant de bacteries marines mesophiles, leur procede de preparation et leurs utilisations en regeneration tissulaire
WO2011026039A1 (fr) * 2009-08-28 2011-03-03 Mary Kay Inc. Compositions de soin de la peau
WO2012001164A1 (fr) * 2010-07-02 2012-01-05 Ifremer (Institut Français De Recherche Pour L'exploitation De La Mer) Exopolysaccharides pour la prévention et la lutte contre la formation de biofilms
WO2017015596A1 (fr) * 2015-07-23 2017-01-26 Mary Kay Inc. Formulations topiques pour la peau
WO2017019265A1 (fr) * 2015-07-30 2017-02-02 Truth Aesthetics LLC Composition topique de soin de jour pour la peau

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"Visual evaluation in vivo of 'complexion radiance' using the CLBTTM sensory methodology", SKIN RESEARCH AND TECHNOLOGY, vol. 10, no. 1, 6 March 2004 (2004-03-06), pages 50
DATABASE GNPD [online] MINTEL; 22 September 2017 (2017-09-22), ANONYMOUS: "Perfect-Gel Foundation SPF 40 PA+++", XP055596711, retrieved from www.gnpd.com Database accession no. 5123739 *
G.H.C. RAGUENES ET AL: "Alteromonas infernus sp. nov., a new polysaccharide-producing bacterium isolated from a deep-sea hydrothermal vent", JOURNAL OF APPLIED MICROBIOLOGY, vol. 82, no. 4, 1 April 1997 (1997-04-01), pages 422 - 430, XP055034307, ISSN: 1364-5072, DOI: 10.1046/j.1365-2672.1997.00125.x *
MATA ET AL: "Characterization of exopolysaccharides produced by three moderately halophilic bacteria belonging to the family Alteromonadaceae", JOURNAL OF APPLIED MICROBIOLOGY, vol. 105, 31 December 2008 (2008-12-31), pages 521 - 528, XP002793853, ISSN: 1364-5072, DOI: 10.1111/j.1365-2672.2008.03789.x *
UCHECHUKWU NWODO ET AL: "Bacterial Exopolysaccharides: Functionality and Prospects", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 13, no. 12, 30 October 2012 (2012-10-30), pages 14002 - 14015, XP055216073, DOI: 10.3390/ijms131114002 *

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