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WO2020131182A3 - Compounds, compositions, and methods for improving assays - Google Patents

Compounds, compositions, and methods for improving assays Download PDF

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Publication number
WO2020131182A3
WO2020131182A3 PCT/US2019/052622 US2019052622W WO2020131182A3 WO 2020131182 A3 WO2020131182 A3 WO 2020131182A3 US 2019052622 W US2019052622 W US 2019052622W WO 2020131182 A3 WO2020131182 A3 WO 2020131182A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
solid support
detection reagent
compositions
compounds
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2019/052622
Other languages
French (fr)
Other versions
WO2020131182A2 (en
Inventor
William Hampton HENLEY
Elizabeth Ann DETHOFF
John Michael Ramsey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of North Carolina at Chapel Hill
Original Assignee
University of North Carolina at Chapel Hill
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of North Carolina at Chapel Hill filed Critical University of North Carolina at Chapel Hill
Priority to US17/048,115 priority Critical patent/US20210214776A1/en
Priority to JP2020558006A priority patent/JP2022501005A/en
Priority to EP19898938.6A priority patent/EP3857228A4/en
Priority to CN202411394208.6A priority patent/CN119492871A/en
Priority to CN201980036497.3A priority patent/CN112189139B/en
Priority to CN202411394209.0A priority patent/CN119224296A/en
Publication of WO2020131182A2 publication Critical patent/WO2020131182A2/en
Publication of WO2020131182A3 publication Critical patent/WO2020131182A3/en
Anticipated expiration legal-status Critical
Priority to JP2024224640A priority patent/JP2025041759A/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided are compounds, compositions, kits, systems, devices, and methods for improving an assay such as, for example, a multiplexed PCR assay (e.g., a multiplexed immuno-PCR assay). A solid support (e.g., a bead) may be provided according to some embodiments of the present invention. The solid support may comprise an encoding agent (e.g., a dye), a nucleic acid sequence (e.g., an oligonucleotide and/or primer); and a molecular recognition element (e.g., an antibody). A detection reagent may be provided according to some embodiments of the present invention. The detection reagent may comprise a molecular recognition element (e.g., an antibody) and a nucleic acid tag. In some embodiments, at least a portion of the nucleic acid sequence of the solid support and at least a portion of the nucleic acid tag of the detection reagent are configured to participate in a nucleic acid amplification process. A solid support and detection reagent may bind to the same target, thereby forming a reagent pair.
PCT/US2019/052622 2018-09-26 2019-09-24 Compounds, compositions, and methods for improving assays Ceased WO2020131182A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
US17/048,115 US20210214776A1 (en) 2018-09-26 2019-09-24 Compounds, compositions, and methods for improving assays
JP2020558006A JP2022501005A (en) 2018-09-26 2019-09-24 Compounds, compositions, and methods for improving the assay
EP19898938.6A EP3857228A4 (en) 2018-09-26 2019-09-24 COMPOUNDS, COMPOSITIONS AND METHODS FOR IMPROVING DOSAGE
CN202411394208.6A CN119492871A (en) 2018-09-26 2019-09-24 Compounds, compositions and methods for improving assays
CN201980036497.3A CN112189139B (en) 2018-09-26 2019-09-24 Compounds, compositions and methods for improving assays
CN202411394209.0A CN119224296A (en) 2018-09-26 2019-09-24 Compounds, compositions and methods for improving assays
JP2024224640A JP2025041759A (en) 2018-09-26 2024-12-20 Compounds, compositions, and methods for improving assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862736525P 2018-09-26 2018-09-26
US62/736,525 2018-09-26

Publications (2)

Publication Number Publication Date
WO2020131182A2 WO2020131182A2 (en) 2020-06-25
WO2020131182A3 true WO2020131182A3 (en) 2020-08-20

Family

ID=71100749

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2019/052622 Ceased WO2020131182A2 (en) 2018-09-26 2019-09-24 Compounds, compositions, and methods for improving assays

Country Status (5)

Country Link
US (1) US20210214776A1 (en)
EP (1) EP3857228A4 (en)
JP (2) JP2022501005A (en)
CN (3) CN119224296A (en)
WO (1) WO2020131182A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9617589B2 (en) 2012-05-25 2017-04-11 The University Of North Carolina At Chapel Hill Microfluidic devices, solid supports for reagents and related methods
EP3957996B1 (en) 2015-10-05 2025-09-17 The University of North Carolina at Chapel Hill Fluidic devices, kits, and solid supports for decoding methods for multiplexing assays
US12234377B2 (en) 2018-05-18 2025-02-25 The University Of North Carolina At Chapel Hill Compositions, devices, and methods for improving a surface property of a substrate
EP3880364B1 (en) 2018-11-13 2024-04-17 The University of North Carolina at Chapel Hill Analysis system for microfluidic devices
CN114994324B (en) * 2021-03-02 2025-06-06 上海泌码生命科学有限公司 Composition, kit, detection method and application for immunoassay
CN115948518A (en) * 2022-07-28 2023-04-11 广州合一生物科技有限公司 Coded microbead for single cell detection and preparation method thereof
CN117405878B (en) * 2023-10-12 2024-06-11 上海领检科技有限公司 A silicon dioxide microsphere composite and its preparation method and use
CN118091110B (en) * 2024-04-03 2025-03-25 中国科学院过程工程研究所 A biological probe for single-molecule immunoassay and detection method and use thereof
CN118006730B (en) * 2024-04-10 2024-08-13 江西省转化医学研究院 Alzheimer disease marker protein immune PCR detection reagent and kit
CN120193049A (en) * 2025-02-27 2025-06-24 上海交通大学医学院附属上海儿童医学中心 A ZO-1 protein quantitative kit and its application

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US20050239108A1 (en) * 2004-02-23 2005-10-27 University Of Maryland, Baltimore Immuno-PCR method for the detection of a biomolecule in a test sample
US20070059690A1 (en) * 2002-05-31 2007-03-15 Amirul Islam "Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands"
WO2014182835A1 (en) * 2013-05-09 2014-11-13 Bio-Rad Laboratories, Inc. Magnetic immuno digital pcr assay

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US20070059690A1 (en) * 2002-05-31 2007-03-15 Amirul Islam "Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands"
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Also Published As

Publication number Publication date
EP3857228A2 (en) 2021-08-04
CN119492871A (en) 2025-02-21
CN119224296A (en) 2024-12-31
WO2020131182A2 (en) 2020-06-25
US20210214776A1 (en) 2021-07-15
JP2022501005A (en) 2022-01-06
CN112189139A (en) 2021-01-05
CN112189139B (en) 2024-10-29
JP2025041759A (en) 2025-03-26
EP3857228A4 (en) 2022-07-06

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