WO2020111297A1 - Cosmetic composition comprising thyme composite extract as active ingredient for moisturizing skin - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a thyme extract, a purslane extract, an Asiatic pennywort extract, and a Houttuynia cordata extract as active ingredients for moisturizing the skin. The skin-moisturizing composition of the present invention promotes the expression of filaggrin and hyaluronic acid genes and exhibits an excellent moisturizing effect and as such, can be advantageously used for skin moisturization in the cosmetic, food, and quasi-drug fields. In addition, the composition of the present invention is very safe to the human body as well as exhibiting very remarkable stability.

Description

Cosmetic composition for moisturizing skin containing thyme complex extract as an active ingredient

The present invention relates to a cosmetic composition for moisturizing the skin, and more particularly, to a cosmetic composition for moisturizing the skin, which contains thyme extract, machihyeon extract, coriander extract, and Eoseongcho extract as active ingredients.

In general, the moisturizing cosmetic composition used for the skin maintains a certain amount of moisture on human hair or skin to make it soft and vibrant, and serves to prevent damage such as cracks and dryness. That is, the cosmetic composition for moisturizing the skin is used for the purpose of beautifying the skin or hair and keeping it healthy by supplying a certain amount of water to the skin or hair or by maintaining the water.

The skin is an organ that plays an essential role in maintaining moisture, and is known to be responsible for approximately 2/3 of the weight in controlling moisture. Therefore, in the field of cosmetics, functional studies related to supply and maintenance of moisture, that is, moisturizing, are actively being conducted. In addition, in recent years, the development of cosmetics that increase the moisturizing power of the skin by actively incorporating physiologically active substances obtained from natural substances or the like into cosmetics to further enhance the skin's own defense function has been actively progressed.

Regarding natural material technology that has a skin moisturizing effect, Patent Publication No. 10-2015-0045049,'A cosmetic composition for moisturizing skin containing a plant extract and a method for manufacturing the same' is a cypress leaf liquid extract, pine needle liquid extract, pine needle leaf The liquid extract, the green tea leaf liquid extract, and the fermented extract of the liquid extract of the medicinal herb have disclosed a skin moisturizing effect, and Patent Publication No. 10-2017-0130865,'Anti-inflammatory or skin moisturizing composition comprising a plant extract,' is a fever. It has been disclosed that a combination of pew extract, honeyweed extract and cucumber extract is effective for anti-inflammatory or moisturizing the skin. However, various physiological activity studies using the complex plant extract are still insufficient, and research on a plant complex extract having a sufficient moisturizing effect to be applicable in an industrial field is not yet performed. In addition, there have been few research results on the combination of plant extracts having a sufficiently moisturizing effect so that it can be developed as a raw material for functional cosmetics or food.

Under these backgrounds, the present inventors conducted a study on the combination of natural substances with excellent skin moisturizing effect, and identified that the complex extract combining thyme extract, machihyeon extract, centella extract, and Eoseongcho extract exhibits excellent moisturizing effect, The present invention has been completed.

Therefore, the main object of the present invention is to provide a cosmetic composition for moisturizing the skin, which includes thyme extract, machihyeon extract, centella extract, and Eoseongcho extract as active ingredients.

Another object of the present invention is to provide a food composition for moisturizing the skin, including thyme extract, machihyeon extract, centella extract, and Eoseongcho extract as active ingredients.

Another object of the present invention is to provide a quasi-drug composition for moisturizing the skin, which includes thyme extract, machihyeon extract, centella extract, and Eoseongcho extract as active ingredients.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to an aspect of the present invention, the present invention provides a cosmetic composition for moisturizing the skin, which includes thyme extract, machihyeon extract, coriander extract, and Eoseongcho extract as active ingredients.

The present inventors conducted a study on a combination of substances having excellent skin moisturizing effect, and it was confirmed that each of the thyme extract, machi extract, coriander extract, and Eoseongcho extract among various plants has an effect on moisturizing the skin. , Machihyeon extract, jujube extract, and a combination extract of Eoseongcho extract significantly increases the production of hyaluronic acid in human keratinocytes, and significantly increases the expression of the moisturizing genes Filaggrin and Hyaluronan synthase-3, It proved to have excellent moisturizing effect.

The "time (Thymus vulgaris)" of the invention refers to a perennial plant of the dicotyledon plant currency neck Lamiaceae, and "Portulaca Oleracea (Portulaca oleracea L.)" means a purslane sentinel of the purslane, "Centella asiatica (Centella asiatica )" refers to the perennial plant of the apiaceae family of mountain birch, Ipanhwa-gun, a dicotyledonous plant, Houttuynia cordata Thunberg)" refers to the upper part of the flowering period of more than three hundred.

The above-mentioned thyme, marchi, coleoptera, and Eoseongcho of the present invention can be extracted from their natural, hybrid or varietal plants, and can also be extracted from plant tissue cultures.

The term used in the present invention, "skin moisturizing" means to increase the feeling of moisture on the skin, and to maintain a moist state. The time extract of the present invention, Machihyeon extract, Cypress extract, and Eoseongcho extract showed excellent water retention effect. From this, the composition comprising the Time extract, Machihyeon extract, Cypress extract, and Eoseongcho extract of the present invention is used for moisturizing the skin. It was confirmed that it can be used as.

As used in the present invention, the term "extract" is an extract obtained by extracting a plant, a dilution or concentrate of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof. It includes extracts of all formulations that can be formed using the extract itself and the extract. The extract of the present invention may be preferably used in a dry powder form after extraction.

In the extraction of the thyme extract of the present invention, Machihyeon extract, Cypress extract, and Eoseongcho extract, the method for extracting the extract is not particularly limited, and can be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method may include a hot water extraction method, an ultrasonic extraction method, a filtration method, and a reflux extraction method, and these may be performed alone or in combination of two or more extraction methods.

In the present invention, the type of the extraction solvent used for extracting the thyme, marchi, bottled grass, and Eoseongcho is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent is water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; And hydrocarbon-based solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture of these may be used, preferably, water, lower alcohol, 1,3-butylene glycol, ethyl acetate may be used alone or in combination of two or more.

In the present invention, the extract extracted with hot water or cold immersion is filtered to remove floating solid particles, filtered using, for example, nylon, or filtered using a freeze filtration method, or used as it is or freeze-dried or hot-air dried, It can be used by drying using spray drying or the like.

In addition, in the cosmetic composition for moisturizing the skin of the present invention, the thyme extract is 0.01 to 10 parts by weight, the machihyeon extract is 0.01 to 2.5 parts by weight, the bottle extract is 0.01 to 7.5 parts by weight, and the total composition is 100 parts by weight Eoseongcho extract may be included in 0.01 to 2.5 parts by weight.

In addition, in the cosmetic composition for moisturizing the skin of the present invention, it is preferable that the thyme extract, machihyeon extract, centella extract, and Eoseongcho extract are mixed in a weight ratio of 4:1:2-4:1, and 4:1:3: Most preferably, it is mixed in a weight ratio of 1. In the embodiment of the present invention, it was confirmed that the composition for moisturizing the skin comprising the complex extract of thyme, machihyeon, coriander and Eoseongcho has no cytotoxicity, and increases the synthesis of hyaluronic acid, thereby improving skin moisturizing effect. Particularly, as a result of confirming the skin moisturizing effect by mixing the extracts in various ratios, it was found that thyme, machihyeon, coriander, and Eoseongcho have the highest synergistic effect on skin moisturizing in the composite extract mixed in a ratio of 4:1:3:1. I could confirm.

The thyme extract of the present invention, Machihyeon extract, Cypress extract, and Eoseongcho extract have excellent skin moisturizing effect, and specifically, it is characterized by promoting the expression of Filaggrin and Hyaluronic acid genes. In addition, the composition of the present invention has the effect of promoting Hyaluronan synthase-3 (HAS-3, skin moisturizing factor) gene expression.

In the cosmetic composition for moisturizing the skin of the present invention, the composition is a solution, external ointment, cream, foam, nutrient longevity, softening longevity, mask pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen Manufactured in a formulation selected from the group consisting of creams, sun oils, suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays It can, but is not limited to this.

In addition, the cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers to be blended with general skin cosmetics, and for example, oil, water, surfactant, moisturizing agent, lower alcohol, Thickeners, chelating agents, pigments, preservatives, fragrances, etc. may be appropriately blended, but are not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures of these can be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof can be used as a carrier component, especially in the case of a spray, additional chloro And propellants such as fluorohydrocarbon, propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-Butyl glycol oil can be used, in particular cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan. have.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used.

When the formulation of the present invention is a soap, alkali metal salt of fatty acid, fatty acid hemiester salt, fatty acid protein hydrolyzate, isethionate, lanolin derivative, aliphatic alcohol, vegetable oil, glycerol, sugar, etc. may be used as a carrier component. Can be.

According to another aspect of the present invention, the present invention provides a food composition for moisturizing the skin, which includes thyme extract, machihyeon extract, coriander extract, and Eoseongcho extract as active ingredients. The thyme extract of the present invention, Machi Prefecture extract, Cypress extract, and Eoseongcho extract, and skin moisturizing are as described above. The food composition may be used in the form of a health functional food, but is not limited thereto.

It may be included in the form of plant extracts, fractions thereof or processed products thereof, including thyme extracts, machihyeon extracts, coriander extracts, and Eoseongcho extracts of the present invention. In addition, the composition may include a food additive that is food-pharmaceutically acceptable in addition to the active ingredient.

In the present invention, "food supplement additive" means a component that can be supplementally added to food, and is added to prepare a health functional food of each formulation, and can be appropriately selected and used by those skilled in the art. Examples of food supplements include flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners , pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonic acid used in carbonated beverages, and the like, but the types of food supplements of the present invention are not limited by the examples.

The food composition of the present invention may include a health functional food. In the present invention, "health functional food" refers to food prepared and processed in the form of tablets, capsules, powders, granules, liquids and pills, etc. using ingredients or ingredients having useful functionality for the human body. Here, the term “functional” means to obtain a useful effect for health purposes such as nutrient control or physiological action on the structure and function of the human body. The health functional food of the present invention can be manufactured by a method commonly used in the art, and in the preparation, it can be prepared by adding raw materials and ingredients that are conventionally added in the art.

In addition, the formulation of the health functional food can also be prepared without limitation as long as the formulation is recognized as a health functional food. The food composition of the present invention can be manufactured in various types of formulations, and has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material, unlike general drugs, and has excellent portability. Health functional foods can be taken as supplements to enhance the skin moisturizing effect.

There is no restriction on the form that the health functional food of the present invention can take, and may include all foods in a common sense, and can be mixed with terms known in the art, such as functional food. In addition, the health functional food of the present invention can be prepared by mixing other appropriate ingredients and known additives that may be included in the food according to the choice of those skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages and There are vitamin complexes, etc., and can be prepared by adding the compound represented by Chemical Formula 1 according to the present invention as a main component to juice, tea, jelly, and juice. It also includes food used as feed for animals.

In addition, according to another aspect of the present invention, the present invention provides a quasi-drug composition for moisturizing the skin, including thyme extract, machihyeon extract, centella extract, and Eoseongcho extract as active ingredients. The thyme extract of the present invention, machihyeon extract, jugyun extract, and Eoseongcho extract, and skin moisturizing are as described above.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient, or diluent as needed in addition to the above components. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as the effects of the present invention are not impaired, for example, fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. It can contain.

Representative examples of pharmaceutically acceptable carriers, excipients or diluents of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, acacia rubber, alginate, calcium phosphate, calcium Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil , Injectable ester, witepsol, macrogol, twin 61, cacao butter, laurid, and the like.

In addition, when used as a quasi-drug extract of the present invention, machihyeon extract, bottleweed extract, and Eoseongcho extract, it may further contain one or more active ingredients exhibiting the same or similar functions. For example, it may contain known moisturizing ingredients. The inclusion of additional moisturizing ingredients can further increase the moisturizing effect of the compositions of the present invention. When adding the above components, skin safety, ease of formulation, and stability of active ingredients according to the combined use may be considered. The quasi-drug composition is a moisturizing ingredient known in the art, retinol acid, TGF, animal placenta-derived protein, betulinic acid and chlorella extract; And one or two or more components selected from the group consisting of these derivatives and various plant extracts. Additional components may be included in 0.001% to 10% by weight relative to the total composition weight, the content range is skin safety, time extract of the present invention, Machihyeon extract, ease of the formulation of the extract, and Eoseongcho extract, etc. It can be adjusted according to the requirements of.

The quasi-drug composition of the present invention may include, but is not limited to, disinfectant cleaners, shower foams, ointments, wipes, coatings, and the like, and formulation methods, dosages, methods of use, and components of quasi-drugs are commonly known in the art. It can be appropriately selected from the technology.

In addition, the thyme extract of the present invention, Machihyeon extract, Cypress extract, and Eoseongcho extract can be used in the method of skin moisturizing, including the step of applying to the skin of an individual. The subject includes, without limitation, mammals, including rats, livestock, and humans.

The features and advantages of the present invention are summarized as follows:

(1) The present invention provides a composition for moisturizing the skin, including thyme extract, machihyeon extract, centella extract, and Eoseongcho extract as active ingredients.

(2) Since the composition for moisturizing the skin of the present invention has excellent moisturizing effect by promoting the expression of Filaggrin and Hyaluronic acid genes, it is usefully used for moisturizing skin in cosmetics, food, and quasi-drugs. It is possible.

(3) In addition, the composition of the present invention is not only very safe for the human body, but also excellent in stability.

1 is a graph showing the measurement of changes in cell viability after treating thyme extract, Machihyeon extract, Cypress extract, and Eoseongcho extract individually on human keratinocytes (HaCaT).

Figure 2 is a graph showing by measuring the change in the amount of production of hyaluronic acid after treatment with human keratinocytes, respectively, thyme extract, Machi Prefecture extract, Cypress extract, and Eoseongcho extract alone.

Figure 3 is a graph showing by measuring the change in the amount of production of hyaluronic acid after treating the complex extracts of thyme, machihyeon, yelp, and Eoseongcho with different combination ratios to human keratinocytes.

Figure 4 is a graph showing by measuring the change in the amount of production of hyaluronic acid after treating the complex extract of thyme, machihyeon, yelp, and Eoseongcho by varying the combination ratio.

5 is a graph showing the expression level of a moisturizing-related gene after treating a complex extract (4:1:3:1 weight ratio) of thyme, machihyeon, medicinal herb, and Eoseongcho to human keratinocytes.

Hereinafter, the present invention will be described in more detail through examples. Since these examples are only for illustrating the present invention, the scope of the present invention is not to be construed as being limited by these examples.

Example 1.Time, March , Bottleweed , And Preparation of Eoseongcho Extract

0.5 kg of thyme, marchi, bottleweed, and Eoseongcho was washed with purified water, dried, then pulverized and refined to obtain a powder. After adding 10 times the weight of ethanol to powdered thyme, marchi, bottled grass, and Eoseongcho, respectively, the mixture was stirred overnight, and then extracted with 50% ethanol solvent, filtered and the supernatant was lyophilized to obtain an extract.

Example 2. Time, March , Bottleweed , And verification of the cytotoxic effect of Eoseongcho extract

The effect of thyme, machihyeon, coriander, and Eoseongcho extracts on the growth of human keratinocytes (HaCaT) was confirmed by the following method.

First, after inoculating human keratinocytes at a concentration of 1×10 ⁵ in a 24 well cell culture dish in a Dulbeccos modified Eagles medium (DMEM) medium, which is a dedicated medium containing 10% fetal bovine serum (Cambrex), for 24 hours. , 37 ℃, incubated in 5% CO ₂ wet conditions. Thereafter, the medium was removed and diluted with serum-free DMEM medium to treat the thyme, marchi, coleoptera, and Eoseongcho extracts prepared in Example 1 by concentration (10, 50, and 100 μg/mL) for 24 hours. . After 24 hours, 1 mg/mL of MTT was treated and after 2 hours, formazan produced by MTT treatment was dissolved in DMSO with DMSO to measure absorbance at 570 nm.

As a result, when treated with thyme, machihyeon, coriander, and Eoseongcho extracts on human keratinocytes, it was confirmed that thyme, machihyeon, juniper, and Eoseongcho extracts do not significantly affect the cell viability of human keratinocytes. Through this, it was confirmed that the extracts of thyme, machihyeon, medicinal herb, and Eoseongcho each did not show toxicity to the human body (see FIG. 1 ).

Example 3. Hyaluronic acid ( Hyaluronan ) Synthesis promotion

3-1. Effect of single extract

The effects of thyme, marchi, coleoptera, and Eoseongcho extracts prepared according to the above examples on hyaluronic acid synthesis were tested.

Human keratinocytes were inoculated at a concentration of about 1×10 ^{5 in} a 1 ml culture solution in a 24 well cell culture dish, and cultured for 24 hours in a 37° C., 5% CO ₂ environment. Subsequently, the medium prepared in Example 1 was replaced with a medium containing thyme, marchi string, coriander, and Eoseongcho extracts (10, 50, and 100 μg/ml) and cultured for 24 hours. Thereafter, the culture solution was harvested and centrifuged for 5 minutes at 4°C and 7,500 rpm for 5 minutes, and then the expression level of Hyaluronan (Hyaluronan kit, R&D Systems, Inc., Minneapolis, MN, USA) was measured using an ELISA method. Was confirmed.

Hyaluronic acid (HA, Hyaluronan, Hyaluronic acid) is mainly synthesized by epidermal keratinocytes and dermal fibroblasts, and is a substance that plays an important role in creating an environment in which cells can function normally by combining with moisture. The reduction of hyaluronic acid in the skin is one of the direct causes of decreased skin elasticity and decreased water content. Therefore, in this experimental example, the amount of hyaluronic acid produced in human keratinocytes was confirmed. As a result, it was confirmed that the production amount of hyaluronic acid (Hyaluronan) was increased in a concentration-dependent manner in the treatment group of thyme, marchi, medicinal herb, and Eoseongcho extracts prepared according to the method of Example 1 compared to the sample-untreated group (Nor). It was confirmed that the ability to promote hyaluronic acid (Hyaluronan) production was excellent in the order of thyme, machihyeon, beeful, and Eoseongcho (see FIG. 2).

3-2. Effects of complex extracts

Hyaluronic acid (Hyaluronan) synthesis changes were measured in the same manner as in Example 3-1, but the effect of these complex extracts was verified by combining thyme, machihyeon, beeful, and eoseongcho.

The complex extracts were used at concentrations of 10, 50, and 100 µg/ml, and the median ratio between the used plant extracts is shown in Table 1 below.

As a result of the experiment, as shown in FIGS. 3 and 4, it was shown that the composite extract of thyme, machihyeon, coriander, and Eoseongcho showed high hyaluronic acid production promoting ability. In particular, excellent hyaluronic acid (Hyaluronan) production promoting ability was observed in the composite extracts of Experimental Examples 5 to 7 in which thyme, marchi string, medicinal herb, and Eoseongcho were mixed at a ratio of 4:1:2 to 4:1, and thyme, marchi string, The best effect was observed in the complex extract of Experimental Example 6 in which the medicinal herb and Eoseongcho were mixed in a ratio of 4:1:3:1.

That is, it shows a synergistic effect of synergistic hyaluronic acid (Hyaluronan) production when using a mixture of thyme, machihyeon, bottled grass, and Eoseongcho extract at a constant ratio as in the present invention than when each extract was used separately. Was confirmed.

Example 4. Filaggrin And Hyaluronan synthase -3 gene expression analysis

In Example 3, the composite extract of Experimental Example 6, which showed that hyaluronic acid (Hyaluronan) production promoting effect was the best, was obtained by Filaggrin (FLG, stratum corneum forming factor) and Hyaluronan synthase-3 (HAS-3, skin moisturizing factor). The effect on gene expression was tested.

First, human keratinocytes were inoculated at a concentration of 1×10 ⁶ cells in DMEM medium containing 10% FBS in a 100 mm cell culture dish, and cultured in a 37° C., 5% CO ₂ incubator for 24 hours. Incubated for 24 hours, the extracts of Experimental Example 6 were diluted in DMEM medium so as to have concentrations of 50 μg/mL and 100 μg/mL, respectively, and the diluted solution prepared was added and cultured for 24 hours under the same conditions. Thereafter, cells were recovered using Trizol reagent (invitrogen, USA) to isolate RNA. Using the RT-PCR method, mRNA expression levels of Filaggrin and hyaluronan synthase-3 were confirmed. The primers used were:

-Filaggrin: Forward 5'-AAG CTT CAT GGT GAT GCG AC-3', Reverse 5'-TCA AGC AGA AGA GGA AGG CA-3'

-Hyaluronan synthase-3: Forward 5′-CCC AGC CAG ATT TGT TGA TG-3′, Reverse 5′-AGT GGT CAC GGG TTT CTT CC-3′

-GAPDH: Forward 5'-ACC ACA GTC CAT GCC ATC AC-3', Reverse 5'-CCA CCA CCC TGT TGC TGT AG-3'.

RT-PCR was performed using the primers described above, and the results are summarized in FIG. 5.

Filaggrin exists in the form of profilaggrin, a protein that forms keratohialin granules in the epidermal granular layer, and then forms a keratinocyte membrane after it is degraded into filaggrin in the final differentiation process of keratinocytes. When the keratin filaments are aggregated to form a hard and flat structure of keratinocytes, it acts as a brick in the skin barrier.

In addition, hyaluronic acid (HA, Hyaluronan, Hyaluronic acid) is a component in the form of a carbohydrate and plays an important role in creating an environment in which cells can function normally by combining with moisture in the dermis and epidermis. Hyaluronic acid, one of the bioactive substances present in the dermal layer and epidermis, is a substance synthesized in fibroblasts and produced by hyaluronic acid synthase.

Therefore, in this example, mRNA expression of pilargreen and hyaluronic acid synthase was confirmed in human keratinocytes directly related to skin moisturization. As a result, compared to the sample-untreated group, in the complex extract-treated group of Experimental Example 6, in which the thyme, marchi string, coleoptera, and Eoseongcho were mixed at a ratio of 4:1:3:1, the mRNA expression levels of filaggrin were respectively It was increased by 389.5% (50 μg/mL) and 607.1% (100 μg/mL), and mRNA expression levels of hyaluronan synthase-3 increased by 30.5% (50 μg/mL) and 46.2% (100 μg/mL), respectively. Was observed.

In addition, even when compared with the thyme single extract, which was observed to be the most effective among the single extracts, in the complex extract treatment group of Experimental Example 6 in which the thyme, marchi string, perilla, and Eoseongcho were mixed in a ratio of 4:1:3:1. The amount of mRNA expression of pilarggrin increased by 23.2% (50 μg/mL) and 76.4% (100 μg/mL), respectively, and the amount of mRNA expression of hyaluronan synthase-3 was 47.8% (50 μg/mL), respectively. ) And 22.0% (100 μg/mL) were observed (see FIG. 5 ).

Through the results as described above, the composition for moisturizing the skin comprising the extract of thyme, marchi string, coriander, and Eoseongcho of the present invention has no cytotoxicity and has an effect of improving skin moisturizing by increasing keratin formation and synthesis of hyaluronic acid. In particular, it was confirmed that the highest synergistic effect on moisturizing the skin was found in a complex extract in which thyme, machihyeon, yelp, and Eoseongcho were mixed in a ratio of 4:1:3:1.

Since the specific parts of the present invention have been described in detail above, it is clear that for those skilled in the art, this specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto.

Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

A cosmetic composition for moisturizing the skin, which includes thyme extract, machihyeon extract, centella extract, and Eoseongcho extract as active ingredients. According to claim 1, The thyme extract, compared to 100 parts by weight of the total composition, is characterized in that 0.01 to 10 parts by weight, the Machihyeon extract is 0.01 to 2.5 parts by weight, the centella extract is 0.01 to 7.5 parts by weight, and the fish vinegar extract is 0.01 to 2.5 parts by weight Cosmetic composition for moisturizing skin. According to claim 1, The thyme extract, Machi Prefecture extract, Centella extract, and Eoseongcho extract are cosmetic compositions for moisturizing the skin, characterized in that they are in a weight ratio of 4:1:2-4:1. According to claim 1, The composition is a cosmetic composition for moisturizing the skin, characterized in that it promotes the expression of filament green (Filaggrin) and hyaluronic acid (Hyaluronic acid) genes. The method according to any one of claims 1 to 4, The composition is a solution, ointment for ointment, cream, foam, nutrient makeup, softening makeup, mask pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension, emulsion, A cosmetic composition for moisturizing skin comprising a paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray. Food composition for moisturizing the skin, including thyme extract, machihyeon extract, coriander extract, and Eoseongcho extract as active ingredients. A quasi-drug composition for moisturizing the skin, including thyme extract, machihyeon extract, centella extract, and Eoseongcho extract as active ingredients.

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