WO2020062521A1 - Bionic digestive tract and preparation method and application for bionic digestive tract - Google Patents

Abstract

A bionic digestive tract and preparation method and application for the bionic digestive tract, relating to the technical field of bionics and the technical field of biology. The bionic digestive tract is prepared by mixing one or more base materials of a silica gel, a latex, or a hydrogel and auxiliary materials of a silicone oil and a curing agent according to a certain mass ratio, i.e., the mass ratio of the base materials, the silicone oil, and the curing agent is 100 : 0.5-10 : 0.5-3.5. The bionic digestive tract has an excellent simulation performance, can stimulate real states of foods, drugs, and microorganisms and the like in a digestive system, and has an application prospect during a study process of the foods and drugs.

Description

Bionic digestive tract, preparation method and application thereof Technical field

The invention relates to a bionic digestive tract, a preparation method and application thereof, and belongs to the field of bionic technology and biotechnology.

Background technique

In the process of food and drug research, objectively and accurately assessing the digestion of food and drugs in the human body is essential to determine the components of food, drugs, and their effects on the human digestive tract.

Human digestive tract in vitro simulation devices (such as SHIME, IViDiS, TIM, DGM, HGS, etc.) are bionic simulation devices for the human digestive system and its digestive environment, and the dynamic behavior of fluids in the digestive tract, which can simulate different samples in an in vitro model The digestion process and the effect of these samples on intestinal microorganisms, so as to provide a lot of valuable data for the development of new functional foods. If it is used as a "pre-test" to predict the living test, it can completely or partially replace the living test. To achieve the purpose of reducing costs and time, improving repeatability and accuracy, and there is no theoretical limit.

Therefore, the human digestive tract in vitro simulation device is becoming more and more critical in the research of food, drugs, and even microorganisms, which provides great convenience for the research of food science and human nutrition. It is unmatched by mouse experiments or human volunteer experiments. of.

The simulated digestive tract (mainly including the stomach, small intestine, and large intestine) is the main body of a human digestive tract in vitro simulation device. The degree of simulation of the human real digestive tract is very important in the simulation performance of the entire human digestive tract in vitro simulation device.

At present, people usually use silicone, latex and other materials to make models of simulated digestive tracts, which can reproduce the digestive environment in the digestive tract well, such as non-sticky, insoluble in water, non-reactive with acid and alkali, high and low temperature resistance, etc.

However, the internal physiological structures of the existing digestive tract models made of silicone and latex are smooth and do not have the function of grinding food. They can only realistically simulate some liquid foods (drinks, milk, etc.) that do not need to be ground. Digestive status in the stomach; for solid (rice, fruit, meat, etc.) or semi-solid (congee, soup, etc.) foods, they can only reflect the function of mixed foods, and they cannot truly reflect the gastrointestinal tract grind and digest food Functions.

Moreover, these digestive tract models are often simply made into an overall structure. For example, they can be molded into a stomach with silicone or latex at one time. These stomachs cannot simulate the "backflow" function between the gastric antrum and the stomach body, resulting in The experimental measurement results are not accurate.

At the same time, the simulation performance of these digestive tract models made of silica gel and latex is actually not satisfactory in terms of hardness, swelling rate, elasticity, etc., and there is still a certain gap with the real digestive tract of humans.

Therefore, there is an urgent need for innovative upgrades of the bionic digestive tract to improve its simulation performance, enhance its digestive environment (non-sticky, insoluble in water, non-reactive with acid and alkali, high and low temperature resistance) and various parts of the digestive tract that simulate the real human digestive tract. The authenticity of the function.

Summary of the Invention

In order to solve the above problems, the present invention provides a bionic digestive tract and a preparation method and application thereof. The bionic digestive tract is based on a certain mass ratio between the substrate (one or more of silica gel, latex or hydrogel) and auxiliary materials (silicone oil and curing agent) (between the substrate, silicone oil and curing agent). The mass ratio is 100: 0.5 ～ 10: 0.5 ～ 3.5). It is prepared after mixing, and its simulation performance is superior. It has strong consistency with human real digestive tract in terms of performance, structure and function, which can simulate food. The real state of drugs, microorganisms, etc. in the digestive system has great application prospects in the research of food and drugs.

The technical solution of the present invention is as follows:

The invention provides a biomimetic material, the components of the biomimetic material include a base material and an auxiliary material; the base material includes one or more of silica gel, latex, or hydrogel; the auxiliary material includes silicone oil and curing Agent.

In one embodiment of the present invention, the mass ratio between the substrate, the silicone oil, and the curing agent is 100: (0.5 to 10): (0.5 to 3.5).

In one embodiment of the present invention, the substrate is silica gel.

In one embodiment of the present invention, the silica gel is transparent silica gel or translucent silica gel.

In one embodiment of the present invention, the curing agent comprises orthosilicate, vinyltriamine, DETA aminoethylpiperazine AE, m-phenylenediamine m-PDAMPD, diaminodiphenylmethane DDM or One or more of HT-972DEH-50.

The invention provides a bionic digestive tract, which is prepared by using a bionic material according to any one of claims 1-3; the digestive tract includes a stomach, a large intestine, an ileum, and a duodenum. , One or more of the jejunum.

In an embodiment of the present invention, when the digestive tract is the stomach, the mass ratio between the base material, the silicone oil, and the curing agent is 100: (5-10): (1-3); or the digestive tract When it is the large intestine, the mass ratio between the substrate, the silicone oil and the curing agent is 100: (3-6): (1 to 3); or when the digestive tract is the ileum, the The mass ratio is 100: (2.5 to 3.5): (0.5 to 1.5); or when the digestive tract is duodenum, the mass ratio between the substrate, the silicone oil and the curing agent is 100: (0.5 to 1.5): (2.5 to 3.5); or when the digestive tract is a jejunum, the mass ratio between the substrate, the silicone oil, and the curing agent is 100: (1.5 to 2.5): (1.5 to 2.5).

In one embodiment of the present invention, the substrate is silica gel.

In one embodiment of the present invention, the silica gel is transparent silica gel or translucent silica gel.

In one embodiment of the present invention, the curing agent comprises orthosilicate, vinyltriamine, DETA aminoethylpiperazine AE, m-phenylenediamine m-PDAMPD, diaminodiphenylmethane DDM or One or more of HT-972DEH-50.

The invention provides a method for preparing a bionic stomach. The method is to first prepare a gastric fundus, a gastric body and a gastric antrum mold having a real gastric fundus, a gastric body, and an internal physiological structure of the gastric antrum, and then bionic the stomach according to a certain thickness. The raw material is smeared on the surface of the gastric fundus, gastric body, and antrum mold and left to stand, so that the bionic gastric raw material is solidified and formed, and the mold is removed to obtain a biomimetic stomach. The gastric fundus, gastric body and gastric antrum molds are filled with plastic materials and filled with the accurate human gastric anatomical model of the medical digestive department. The plastic material is cured and molded to obtain a gastric fundus, gastric body and gastric antrum mold having a real physiological structure of the gastric fundus, gastric body, and internal antrum of the stomach; the bionic gastric material is based on a certain mass ratio, The auxiliary materials are mixed and put into a vacuum machine to evacuate the foam; the substrate comprises one or more of silica gel, latex or hydrogel; the auxiliary materials And a curing agent comprising a silicone oil.

In one embodiment of the present invention, the plastic material includes clay, paraffin, glass, or steel.

In one embodiment of the present invention, the clay is soft clay.

In one embodiment of the present invention, the preparation of the gastric fundus, gastric body, and gastric antrum molds with real physiological structures of the gastric fundus, gastric body, and internal antrum of the stomach is to put soft clay mud into and fill the medical digestive department respectively. Accurate human stomach anatomical model of the stomach base, stomach body and gastric antrum. Then remove the accurate human stomach anatomy model from the Medical Gastroenterology Department and roast the soft clay to solidify it. Cool naturally to prevent the mold from cooling quickly to produce cracks. Gastric fundus, gastric body, and gastric antrum mold with real physiological structure of the gastric fundus, gastric body, and antrum.

In one embodiment of the present invention, the accurate human stomach anatomy model in the medical digestive department is a real stomach 1: 1 hollow model.

In one embodiment of the present invention, the baking time is 15-30 minutes.

In one embodiment of the present invention, the mass ratio between the substrate, the silicone oil, and the curing agent is 100: (5 to 10): (1 to 3).

In one embodiment of the present invention, a mass ratio between the substrate, the silicone oil, and the curing agent is 100: 8: 2.

In one embodiment of the present invention, the substrate is silica gel.

In one embodiment of the present invention, the silica gel is transparent silica gel or translucent silica gel.

In one embodiment of the present invention, the curing agent comprises orthosilicate, vinyltriamine, DETA aminoethylpiperazine AE, m-phenylenediamine m-PDAMPD, diaminodiphenylmethane DDM or One or more of HT-972DEH-50.

In one embodiment of the present invention, the conditions for the vacuum evacuation and degassing are a vacuum negative pressure of -0.1 to 0.09 MPa, and a time of 5 to 10 minutes.

In one embodiment of the present invention, the conditions for the vacuum evacuation and degassing are a vacuum pressure of 0.09 MPa and a time of 7 minutes.

In an embodiment of the present invention, the thickness of the bionic gastric raw material applied on the surface of the stomach bottom, stomach body, and gastric antrum mold is 1 to 3 mm.

In one embodiment of the present invention, the thickness of the bionic gastric raw material applied on the surface of the gastric fundus, gastric body, and gastric antrum mold is 2 mm.

In one embodiment of the present invention, the standing time is 2 to 4 hours.

In one embodiment of the present invention, the standing time is 3h.

The invention provides a bionic stomach prepared by applying the above method.

The invention provides a method for preparing a bionic large intestine. The method is to first prepare a large intestine mold having a true internal physiological structure of the large intestine, and then apply the bionic large intestine raw material on the surface of the large intestine mold according to a certain thickness and leave it to rest, so that the bionic large intestine. The raw materials are solidified and formed, and the mold is removed to obtain a bionic large intestine. Among them, a large intestine mold having a true internal physiological structure of the large intestine is prepared by placing and filling a plastic material into a large intestine of a precise human anatomy model of the medical digestive department, and then removing the medical digestive department. Accurate human intestinal anatomical model of the large intestine and solidifying the plastic material to obtain a large intestine mold with a true internal physiological structure of the large intestine; the bionic large intestine material is obtained by mixing a base material and an auxiliary material for making a bionic large intestine according to a certain mass ratio It is obtained by putting into a vacuum machine to evacuate the bubble; the substrate includes one or more of silica gel, latex, or hydrogel; and the auxiliary material includes silicone oil and a curing agent.

In one embodiment of the present invention, the plastic material includes clay, paraffin, glass, or steel.

In one embodiment of the present invention, the clay is soft clay.

In one embodiment of the present invention, the preparation of a large intestine mold having a true internal physiological structure of the large intestine is to put soft clay into and fill the large intestine of the accurate human anatomy model of the medical digestive department, and then remove the medical accurate The large intestine of the human intestinal anatomical model is baked with soft clay to solidify it, and naturally cooled to prevent the mold from rapidly cooling to produce cracks, and a large intestine mold with a true internal physiological structure of the large intestine is obtained.

In one embodiment of the present invention, the accurate human intestinal anatomy model of the medical digestive department is a real 1: 1 hollow model of the intestine.

In one embodiment of the present invention, the large intestine mold has a diameter of 4 to 5 cm and a length of 20 to 30 cm.

In one embodiment of the present invention, the baking time is 15-30 minutes.

In one embodiment of the present invention, the mass ratio between the substrate, the silicone oil, and the curing agent is 100: (3 to 6): (1 to 3).

In an embodiment of the present invention, a mass ratio between the substrate, the silicone oil, and the curing agent is 100: 4: 2.

In one embodiment of the present invention, the substrate is silica gel.

In one embodiment of the present invention, the silica gel is transparent silica gel or translucent silica gel.

In one embodiment of the present invention, the curing agent comprises orthosilicate, vinyltriamine, DETA aminoethylpiperazine AE, m-phenylenediamine m-PDAMPD, diaminodiphenylmethane DDM or One or more of HT-972DEH-50.

In one embodiment of the present invention, the conditions for the vacuum evacuation and degassing are a vacuum negative pressure of -0.1 to 0.09 MPa, and a time of 5 to 10 minutes.

In one embodiment of the present invention, the conditions for the vacuum evacuation and degassing are a vacuum pressure of 0.09 MPa and a time of 7 minutes.

In an embodiment of the present invention, the thickness of the bionic large intestine raw material applied on the surface of the large intestine mold is 1 to 3 mm.

In one embodiment of the present invention, the thickness of the bionic large intestine raw material applied on the surface of the large intestine mold is 2 mm.

In one embodiment of the present invention, the standing time is 2 to 4 hours.

In one embodiment of the present invention, the standing time is 3h.

The invention provides a bionic large intestine prepared by applying the above method.

The invention provides a method for preparing a bionic ileum. The method is to first prepare an ileal mold having a true internal physiological structure of the ileum and retaining needles, and then apply the bionic ileal raw material to the ileal mold and the human ileum according to a certain thickness. The side where the anatomical model is in contact is left to stand, so that the raw material of the bionic ileum is solidified and formed. The needle is pulled out and the mold is removed to obtain a hollow bionic ileum with small intestinal villi and a hollow body. Among them, a real ileal internal physiological structure is prepared and retained. Needle-shaped ileum molds are filled with plastic materials and filled with accurate anatomical models of human ileum in medical digestive department, and then the human ileal anatomical models are removed, and each of the plastic materials corresponds to the small intestine villi on the human ileal anatomical model A small hole is pierced with a needle in the depression of the recess, and finally the needle is kept on the plastic material and the plastic material is cured and molded to obtain an ileal mold having a true internal ileum internal physiological structure and retaining the needle; Bionic ileal material is the basis for making bionic ileum according to a certain mass ratio. After mixing and put in a vacuum auxiliary machine vacuum bubble row obtained;

Or the method is to first prepare an ileal mold with a real internal ileal internal physiological structure and retain needles, and then apply the bionic ileal raw material on the side of the ileal mold that is in contact with the human ileal anatomical model according to a certain thickness and let it stand, The bionic ileum raw material is solidified and formed, and the needle is pulled out without taking off the mold to obtain a hollow bionic ileum with small intestinal villi, and then covering the side of the bionic ileum that is not in contact with the ileal mold. The bionic ileal raw material is applied to the side of the separator that does not contact the bionic ileum with a certain thickness and left to stand, so that the bionic ileal raw material applied on the surface of the separator is solidified and formed, and the separator is removed and the mold is removed to obtain a ileal physiology. Bionic ileum with a structure of villi and secretion of intestinal fluid; among which, an ileum mold having a real internal ileum internal physiological structure and retaining needles is prepared, and a plastic material is put in and filled with a precise human ileal anatomical model in the medical digestive department, and then removed Human ileum anatomical model, then in each of the plastic materials with the human ileum On the cutaway model, the small intestine corresponding to the villi of the small intestine punctures small holes with needles. Finally, the needles are kept on the plastic material and the plastic material is cured and shaped to obtain a true ileal internal physiological structure and retain the needle shape. The ileal mold of the object; the bionic ileal material is obtained by mixing the base material and the auxiliary material for making the bionic ileum according to a certain mass ratio and putting it in a vacuum machine to evacuate the foam;

The substrate includes one or more of silica gel, latex, or hydrogel; and the auxiliary material includes silicone oil and / or a curing agent.

In one embodiment of the present invention, the accurate human intestinal anatomy model of the medical digestive department is a real 1: 1 hollow model of the intestine.

In one embodiment of the present invention, the plastic material includes clay, paraffin, glass, or steel.

In one embodiment of the present invention, the plastic material includes clay, paraffin, glass, or steel.

In one embodiment of the present invention, the clay is soft clay.

In one embodiment of the present invention, the preparation of an ileal mold having a true internal ileal internal physiological structure and retaining needles is to place and fill a soft clay mud into a precise human ileal anatomical model in the medical digestive department, and then remove it. Anatomical model of human ileum, and then use a needle to poke out a small hole in each depression corresponding to the small intestine villi on the human ileal anatomical model. Finally, keep the needle on the soft clay and keep it soft. The clay is baked to solidify and shape, and naturally cooled to prevent the mold from rapidly cooling to generate cracks, to obtain an ileal mold that has the true internal physiological structure of the ileum and retains needles.

In one embodiment of the present invention, the baking time is 15-30 minutes.

In one embodiment of the present invention, the diameter of the small hole is 0.2 to 1 mm.

In one embodiment of the present invention, when a small hole is punctured in each of the depressions corresponding to the small intestine villi on the anatomical model of human ileum, the needles should penetrate the soft clay and make The end of the needle is at least 2 mm beyond the side where the soft clay touches the human ileal anatomical model.

In one embodiment of the present invention, the ileum mold has a diameter of 1.5 to 2.5 cm and a length of 20 to 30 cm.

In one embodiment of the present invention, the mass ratio between the substrate, the silicone oil, and the curing agent is 100: (2.5 to 3.5): (0.5 to 1.5).

In one embodiment of the present invention, the mass ratio between the substrate and the auxiliary material is 100: 3: 1.

In one embodiment of the present invention, the substrate is silica gel.

In one embodiment of the present invention, the silica gel is transparent silica gel or translucent silica gel.

In one embodiment of the present invention, the curing agent comprises orthosilicate, vinyltriamine, DETA aminoethylpiperazine AE, m-phenylenediamine m-PDAMPD, diaminodiphenylmethane DDM or One or more of HT-972DEH-50.

In one embodiment of the present invention, the conditions for the vacuum evacuation and degassing are a vacuum negative pressure of -0.1 to 0.09 MPa, and a time of 5 to 10 minutes.

In one embodiment of the present invention, the conditions for the vacuum evacuation and degassing are a vacuum pressure of 0.09 MPa and a time of 7 minutes.

In one embodiment of the present invention, the thickness of the bionic ileal raw material applied on the surface of the ileal mold is 2 to 3 mm.

In one embodiment of the present invention, the thickness of the bionic ileal raw material applied on the surface of the ileal mold is 2.5 mm.

In one embodiment of the present invention, the standing time is 2 to 4 hours.

In one embodiment of the present invention, the standing time is 3h.

In one embodiment of the present invention, the separator comprises an oily paper or a cling film.

In one embodiment of the present invention, the thickness of the bionic ileal raw material applied on the surface of the spacer is 2 to 3 mm.

In one embodiment of the present invention, the thickness of the bionic ileal raw material applied on the surface of the spacer is 2.5 mm.

The invention provides a bionic ileum prepared by applying the above method.

The invention provides a method for preparing a bionic duodenum. The method is to first prepare an ileum mold having a true internal physiological structure of the duodenum and retaining needles, and then bionic the duodenum according to a certain thickness. The intestinal raw material is smeared on the side where the duodenal mold is in contact with the human duodenal anatomical model and left to stand, so that the bionic duodenal raw material is solidified and formed. The needle is pulled out and the mold is removed to obtain the intestinal villi. And it is a hollow bionic duodenum; in which, a duodenum mold having a real internal physiological structure of the duodenum and retaining needles is prepared, and a plastic material is put in and filled with a precise human twelve in the medical digestive department. Anatomy model of the duodenum, then remove the anatomical model of the duodenum of the human body, and then use a needle to poke out a small hole in each depression of the plastic material corresponding to the small intestine villi on the human duodenal anatomical model. The needles continue to be retained on the plastic material and the plastic material is cured and formed to obtain a duodenal mold having a true internal physiological structure of the duodenum and retaining the needles. ; After the biomimetic material is a duodenum according to a certain ratio of the mass production base bionic duodenum were mixed and placed in a vacuum auxiliary machine vacuum bubble row obtained;

Or the method is to first prepare an ileum mold with the actual internal physiological structure of the duodenum and retain needles, and then apply the bionic duodenal raw material to the duodenum mold and the human duodenum according to a certain thickness. The intestinal anatomical model is in contact with the side and allowed to stand, so that the bionic duodenal raw material is solidified and formed. The needles are pulled out without taking off the mold to obtain a hollow bionic duodenum with small intestinal villi and hollow. The side of the duodenum that is not in contact with the duodenal mold is covered with a layer of spacer, and the bionic duodenal raw material is smeared on the side that does not contact the bionic duodenum according to a certain thickness. The bionic duodenum raw material applied on the surface of the spacer is solidified and formed, and the spacer is removed and the mold is removed to obtain a bionic duodenum with duodenal physiological structure villi and secretion of intestinal fluid; Among them, a duodenum mold having a true internal physiological structure of the duodenum and retaining needles is prepared by filling and filling a plastic material with accurate human duodenum anatomy in the medical digestive department Model, then remove the human duodenal anatomical model, and then poke small holes with needles in the depressions corresponding to the small intestinal villi on the human duodenal anatomical model, and finally pierce the needles Continue to remain on the plastic material and solidify and mold the plastic material to obtain an ileum mold that has the true internal physiological structure of the duodenum and retains needles; the bionic duodenal material is made according to a certain quality ratio The base material and auxiliary materials of the bionic duodenum are mixed and put into a vacuum machine to evacuate and evacuate;

The substrate includes one or more of silica gel, latex, or hydrogel; and the auxiliary material includes silicone oil and / or a curing agent.

In one embodiment of the present invention, the accurate human intestinal anatomy model of the medical digestive department is a real 1: 1 hollow model of the intestine.

In one embodiment of the present invention, the plastic material includes clay, paraffin, glass, or steel.

In one embodiment of the present invention, the plastic material includes clay, paraffin, glass, or steel.

In one embodiment of the present invention, the clay is soft clay.

In one embodiment of the present invention, the preparation of a duodenum mold having a true internal physiological structure of the duodenum and retaining needles is to place soft clay into and fill the precise human body of the medical digestive department. Anatomy model of duodenum, then remove the anatomy model of human duodenum, and then poke small holes with needles in the depressions corresponding to the small intestinal villi on the duodenum anatomy model of human body. Finally, the needles are kept on the soft clay, and the soft clay is baked to solidify and shape, and naturally cooled to prevent the mold from cooling quickly to produce cracks. The actual physiological structure of the duodenum is retained and the needles are retained. Ileum mold.

In one embodiment of the present invention, the baking time is 15-30 minutes.

In one embodiment of the present invention, the diameter of the small hole is 0.2 to 1 mm.

In one embodiment of the present invention, when a small hole is punctured in each of the depressions corresponding to the small intestinal villi on the duodenum anatomical model of the human body, the needles should penetrate the soft ceramic Mud and make the end of the needle at least 2mm beyond the side where the soft clay touches the human duodenal anatomical model.

In one embodiment of the present invention, the duodenum mold has a diameter of 3 to 4 cm and a length of 20 to 30 cm.

In one embodiment of the present invention, a mass ratio between the substrate, the silicone oil, and the curing agent is 100: (0.5 to 1.5): (2.5 to 3.5).

In one embodiment of the present invention, the mass ratio between the substrate and the auxiliary material is 100: 1: 3.

In one embodiment of the present invention, the substrate is silica gel.

In one embodiment of the present invention, the silica gel is transparent silica gel or translucent silica gel.

In one embodiment of the present invention, the curing agent comprises orthosilicate, vinyltriamine, DETA aminoethylpiperazine AE, m-phenylenediamine m-PDAMPD, diaminodiphenylmethane DDM or One or more of HT-972DEH-50.

In one embodiment of the present invention, the conditions for the vacuum evacuation and degassing are a vacuum negative pressure of -0.1 to 0.09 MPa, and a time of 5 to 10 minutes.

In one embodiment of the present invention, the conditions for the vacuum evacuation and degassing are a vacuum pressure of 0.09 MPa and a time of 7 minutes.

In an embodiment of the present invention, the thickness of the bionic duodenal raw material applied on the surface of the duodenal mold is 1 to 2 mm.

In one embodiment of the present invention, the thickness of the bionic duodenal raw material applied on the surface of the duodenal mold is 1.5 mm.

In one embodiment of the present invention, the standing time is 2 to 4 hours.

In one embodiment of the present invention, the standing time is 3h.

In one embodiment of the present invention, the separator comprises an oily paper or a cling film.

In one embodiment of the present invention, the thickness of the bionic duodenal raw material applied on the surface of the spacer is 1 to 5 mm.

In one embodiment of the present invention, the thickness of the bionic duodenal raw material applied on the surface of the spacer is 1.5 mm.

The invention provides a bionic duodenum prepared by applying the above method.

The invention provides a method for preparing a bionic jejunum. The method is to first prepare a jejunum mold that has the actual physiological structure of the jejunum and retains needles, and then apply the bionic jejunum raw material to the jejunum mold and the human jejunum according to a certain thickness. The contacting side of the anatomical model is left to stand to solidify the raw material of the bionic jejunum. The needle is pulled out and the mold is removed to obtain a hollow bionic jejunum with small intestinal villi and hollow; among which, the real jejunum internal physiological structure is prepared and retained. Needle-shaped jejunum mold is to put plastic material into and fill the precise human jejunum anatomical model of medical digestive department, then remove the human jejunum anatomical model, and then each of the plastic material corresponds to the small intestine villi on the human jejunum anatomical model A small hole is pierced with a needle in the depression of the hollow, and finally the needle is kept on the plastic material and the plastic material is cured and formed to obtain a jejunum mold having a real jejunum internal physiological structure and retaining the needle; The bionic jejunum material is based on a certain quality ratio. After mixing and put in a vacuum auxiliary machine vacuum bubble row obtained;

Or the method is to first prepare a jejunum mold that has the actual physiological structure of the jejunum and retains needles, and then apply the bionic jejunum raw material on the side of the jejunum mold that is in contact with the jejunum anatomical model of the human body according to a certain thickness and let it stand. The bionic jejunum raw material is solidified and shaped, the needle is pulled out without taking off the mold, and a bionic jejunum with small intestinal villi and hollow is obtained, and then the side of the bionic jejunum that is not in contact with the jejunum mold is covered with a layer of spacers. The bionic jejunum raw material is applied to the side where the spacer is not in contact with the bionic jejunum with a certain thickness and left to stand, so that the bionic jejunal raw material applied on the surface of the spacer is solidified and formed, the spacer is removed, and the mold is removed to obtain a jejunum physiology A bionic jejunum with a structure of villi and secretion of intestinal fluid. Among them, a jejunum mold having a real physiological structure of the jejunum and retaining needles is prepared, and a plastic material is put in and filled with a precise human jejunum anatomical model of the medical digestive department, and then removed Human jejunum anatomical model, and then in each of the plastic materials with the human jejunum On the anatomical model, the depressions corresponding to the small intestinal villi are used to poke small holes with needles. Finally, the needles are kept on the plastic material and the plastic material is cured and shaped to obtain the true physiological structure of the jejunum and the needle shape. The jejunum mold of the object; the bionic jejunum material is obtained by mixing the base material and the auxiliary material for making the bionic jejunum according to a certain mass ratio and putting it in a vacuum machine to evacuate the bubble;

The substrate includes one or more of silica gel, latex, or hydrogel; and the auxiliary material includes silicone oil and / or a curing agent.

In one embodiment of the present invention, the accurate human intestinal anatomy model of the medical digestive department is a real 1: 1 hollow model of the intestine.

In one embodiment of the present invention, the plastic material includes clay, paraffin, glass, or steel.

In one embodiment of the present invention, the plastic material includes clay, paraffin, glass, or steel.

In one embodiment of the present invention, the clay is soft clay.

In one embodiment of the present invention, the preparation of the jejunum mold having the real internal structure of the jejunum and retaining the needles is to place and fill the soft clay mud with a precise human jejunum anatomical model in the medical digestive department, and then remove it Anatomical model of human jejunum, and then use needles to poke small holes in each depression of soft terracotta clay that corresponds to the small intestine villi on the anatomical model of human jejunum. Finally, keep the needles on the soft clay and soften the clay The clay is baked to solidify and mold, and naturally cooled to prevent the mold from rapidly cooling to produce cracks, to obtain a jejunum mold that has the real physiological structure of the jejunum and retains needles.

In one embodiment of the present invention, the baking time is 15-30 minutes.

In one embodiment of the present invention, the diameter of the small hole is 0.2 to 1 mm.

In an embodiment of the present invention, when a small hole is punctured in each of the depressions corresponding to the small intestine villi on the jejunum anatomical model of the human body, the needles should penetrate the soft clay and make The end of the needle is at least 2 mm beyond the side where the soft clay and the human jejunum anatomical model are in contact.

In one embodiment of the present invention, the jejunum mold has a diameter of 2 to 3 cm and a length of 20 to 30 cm.

In one embodiment of the present invention, the mass ratio between the substrate, the silicone oil, and the curing agent is 100: (1.5 to 2.5): (1.5 to 2.5).

In one embodiment of the present invention, the mass ratio between the substrate and the auxiliary material is 100: 2: 2.

In one embodiment of the present invention, the substrate is silica gel.

In one embodiment of the present invention, the silica gel is transparent silica gel or translucent silica gel.

In one embodiment of the present invention, the curing agent comprises orthosilicate, vinyltriamine, DETA aminoethylpiperazine AE, m-phenylenediamine m-PDAMPD, diaminodiphenylmethane DDM or One or more of HT-972DEH-50.

In one embodiment of the present invention, the conditions for the vacuum evacuation and degassing are a vacuum negative pressure of -0.1 to 0.09 MPa, and a time of 5 to 10 minutes.

In one embodiment of the present invention, the conditions for the vacuum evacuation and degassing are a vacuum pressure of 0.09 MPa and a time of 7 minutes.

In one embodiment of the present invention, the thickness of the bionic jejunum raw material applied on the surface of the jejunum mold is 1.5 to 2.5 mm.

In an embodiment of the present invention, the thickness of the bionic jejunum raw material applied on the surface of the jejunum mold is 2 mm.

In one embodiment of the present invention, the standing time is 2 to 4 hours.

In one embodiment of the present invention, the standing time is 3h.

In one embodiment of the present invention, the separator comprises an oily paper or a cling film.

In one embodiment of the present invention, the thickness of the bionic jejunum raw material applied on the surface of the spacer is 1.5 to 2.5 mm.

In one embodiment of the present invention, the thickness of the bionic jejunum raw material applied on the surface of the spacer is 2 mm.

The invention provides a bionic jejunum prepared by applying the above method.

Beneficial effects:

(1) The biomimetic digestive tract prepared by using the biomimetic material of the present invention has superior performance, has strong consistency in performance, structure, and function with the human real digestive tract, and can simulate food, medicine, microorganisms, etc. The true state of the digestive system has great application prospects in the research of food and medicine.

(2) The bionic digestive tract prepared by using the bionic material of the present invention can be transparent or translucent, and can realize the real-time and real-time observation of the internal digestive state of the digestive tract;

(3) The bionic digestive tract prepared by using the bionic material of the present invention has the advantages of no stickiness, insolubility in water, non-reaction with acid and alkali, high and low temperature resistance, etc., and can well reproduce the digestive and absorbing environment in the digestive tract;

(4) The simulation performance of the bionic stomach of the present invention is superior (Shore A hardness is 68 ± 5, expansion rate is 36 ± 5%, elasticity is 550 ± 50%), and it has the real physiological structure of the real inner wall of the human stomach, which can realize the stomach The function of the ground food has strong consistency in performance, structure, and function with the human real stomach; and the bionic stomach of the present invention separates the bionic gastric antrum and the gastric body, and separates the separated gastric antrum and By giving certain pressure to the gastric body, the function of "backspray" between the gastric body and gastric antrum can be realized;

(5) The bionic large intestine of the present invention has superior simulation performance (Shore A hardness of 60 ± 5, expansion rate of 31 ± 5%, and elasticity of 460 ± 50%). It has the morphology and physiological structure of a human large intestine, which can be real Simulates the digestive environment in the human large intestine. For example, the bionic large intestine has circular folds inside it, which can increase the surface area of the intestine, so that the bacterial flora in the large intestine can better digest and absorb food;

(6) The simulation performance of the bionic ileum, duodenum and jejunum of the present invention is superior (Ileum: Shore A hardness 32 ± 5, expansion rate 28 ± 5%, elasticity 220 ± 50%; duodenum: (Shore A hardness is 40 ± 5, expansion rate is 22 ± 5%, elasticity is 250 ± 50%; jejunum: Shore A hardness is 36 ± 5, expansion ratio is 25 ± 5%, elasticity is 250 ± 50%) The morphology and physiological structure of the human intestinal tract can realistically simulate the digestive environment in the human intestinal tract; the bionic ileum, duodenum and jejunum of the present invention have circular folds inside, which can increase the surface area of the intestine, which can in turn For the microbial flora in the ileum to better digest and absorb food; the bionic ileum, duodenum, and jejunum of the present invention have small intestinal villi and the small intestinal villi can have a double-layered hollow structure, and the intestinal fluid can be filled into the hollow portion. The small intestine villi automatically secrete intestinal fluid during peristalsis, which greatly reproduces the function of digestion and absorption in the intestine.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1: Photograph of gastric fundus of the present invention;

Figure 2: Photo of the stomach body of the present invention;

Figure 3: Photo of gastric antrum of the present invention;

Figure 4: Overall picture of the stomach of the present invention;

Figure 5: Frontal photos of bionic ileum, duodenum and jejunum of the present invention;

Figure 6: Photographs of the reverse side of the bionic ileum, duodenum and jejunum of the present invention;

Figure 7: Photographs of villi of the small intestine of the bionic ileum, duodenum and jejunum of the present invention.

detailed description

The detection methods involved in the following examples are as follows:

Intragastric area detection method:

S = 2π (r ₁ h ₁ + r ₂ h ₂ + r ₃ h ₃ ) + n ₁ (π-1) r ₁ h ₁ + n ₂ (π-1) r ₂ h ₂ + n ₃ (π-1 ) r ₃ h ₃ ;

Among them, S is the area of the stomach, r ₁ is the radius of the gastric fundus, h ₁ is the height of the gastric fundus, n ₁ is the number of the gastric wall, r ₂ is the radius of the gastric body, h ₂ is the height of the gastric body, and n ₂ is the number of the gastric wall , R ₃ is the radius of the gastric antrum, h ₃ is the height of the gastric antrum, and n ₃ is the number of gastric antrum.

Intestinal area detection method:

S = 2πr ₁ h ₁ + n (π-1) r ₂ h ₂ ;

Among them, S is intestinal area, r ₁ is intestinal radius, h ₁ is intestinal height, n is intestinal wall number, r ₂ is intestinal wall radius, and h ₂ is intestinal wall height.

Food grinding function testing method:

Fix the obtained bionic gastric fundus, the bionic gastric body, and the bionic gastric antrum / bionic stomach as a whole through the flange interface of the pipe to the gastric body visualization biomimetic reactor (see the invention patent publication number CN108088966A for gastric body visualization biomimetic reactor), Take 100g of raw rice with a particle size of about 1mm, add it to the bionic stomach, run the reactor for 10min, take out the rice, and observe whether the particle size becomes smaller.

Back spray function detection method:

The bionic stomach is fixed to the gastric body visualization bionic reactor (see the invention patent for publication number CN108088966A for gastric body visualization bionic reactor), 100 ml of milk is added, and the water flow solenoid valve of the reactor is opened by operating the reactor to give pressure to the bionic gastric antrum To see if the "backspray function" of milk in the gastric antrum can be sprayed back to the stomach body.

Transparency detection method:

Use visual inspection to observe whether the silica gel is transparent and translucent, with bubbles, and stretch the silica gel to see if it is whitish and whitish is opaque. Put food such as rice and corn in the silica gel to see if it can be clearly seen The appearance of the food.

If the surface of the food can be clearly seen, the transparency is good; if the outline of the food can be seen, the transparency is moderate; if the object of the food cannot be seen, the transparency is poor.

Hardness (Shore hardness) detection method:

The measurement was performed with a Shore hardness tester, and the pressure needle of the hardness tester was pressed against the surface of the stomach, and the depth of the pressure needle pressed into the surface of the stomach was measured.

Expansion rate detection method:

Pe = (W * m / A) × 100%;

Among them, Pe is the expansion rate, W is the total equilibrium load, A is the area of the silica gel, and m is the leverage ratio of the pressurized equipment.

Elasticity detection method:

A tensile test was performed on the silicone rubber on a tensile tester, and the axial and lateral elongation of the silicone rubber was measured until the silicone rubber broke.

C = (D-d) × 100% / d;

Among them, C is the elastic modulus, D is the elongation amount, and d is the initial length of the silica gel.

Function test of villi secreting intestinal fluid:

The obtained bionic duodenum was fixed in the intestinal visualization bionic reactor through the flange interface of the pipeline (see the intestinal visualization bionic reactor for the invention patent CN108318625A), and 100 ml of pancreatic juice was added to the silicone bionic duodenum During operation, the reactor was run for 10 minutes, and the volume of secreted pancreatic juice was observed.

Example 1.1: Preparation of bionic stomach

(1) Preparation of clay clay: repeatedly kneading the soft clay clay to obtain soft clay clay with good ductility;

(2) Stomach bottom mold pressing: Put the kneaded soft clay into the accurate human stomach anatomical model of the medical digestive department, squeeze it hard, and remove the human stomach anatomical model to obtain the real internal gastric structure. Soft clay mud bottom mold;

(3) Baking the stomach bottom mold: Put the stomach bottom mold in the oven and bake for 15 minutes to solidify the stomach bottom mold and cool it naturally to obtain a bionic stomach bottom mold;

(4) Stomach body mold compression: Put the kneaded soft clay into the stomach of the accurate human anatomical model of human stomach in the medical digestive department, squeeze it hard, remove the human anatomical model of the human stomach, and obtain Soft clay mud stomach mold;

(5) Stomach body mold roasting and molding: Put the stomach body mold into the oven and bake for 15 minutes, solidify the stomach body mold, cool naturally, and obtain a bionic stomach body mold;

(6) Stomach antrum mold compression: Put the kneaded soft clay into the accurate human gastric anatomical model of gastrointestinal medicine, and squeeze it strongly to remove the human gastric anatomical model to obtain a true internal antrum physiological structure. Soft clay mud antrum mold;

(7) Stomach antrum mold baking molding: Put the antrum mold into the oven and bake for 15 minutes, solidify the antrum mold, cool naturally, and obtain a bionic antrum mold;

(8) Preparation of silica gel: Put transparent silica gel, silicone oil and curing agent orthosilicate according to 100: 8: 2 in a beaker, stir thoroughly and mix well;

(9) Put the evenly mixed silica gel into a vacuum machine, with a negative pressure of 0.09 MPa, and evacuate the foam for 5 minutes to obtain a bubble-free silica gel;

(10) Apply the above-mentioned bionic gastric fundus, gastric body, and gastric antrum mold surface evenly with bubble-free silica gel to a thickness of about 1 mm;

(11) The three uniformly applied molds are allowed to stand for 2 hours to completely solidify and mold. After removing the molds, they are subjected to a certain trimming treatment to obtain a silicone bionic gastric fundus, a silicone bionic gastric body and a silicone bionic gastric antrum.

Example 1.2: Preparation of bionic stomach

Based on Example 1.1, the mold baking time in steps (3), (5), and (7) was changed to 20 minutes; the curing agent in step (8) was changed to diaminodiphenylmethane DDM At the same time, change the mass ratio of transparent silicone, silicone oil and curing agent to 100: 7: 1.5; change the defoaming time in step (9) to 10 minutes; change the smear thickness in step (10) to 2mm; The standing time in step (11) becomes 4 hours.

Example 1.3: Preparation of bionic stomach

Based on Example 1.1, the mold baking time in steps (3), (5), and (7) was changed to 30 minutes; the curing agent in step (8) was changed to vinyltriamine, and at the same time The mass ratio of the transparent silica gel, the silicone oil, and the curing agent becomes 100: 9: 3; the coating thickness in step (10) is changed to 3 mm; and the standing time in step (11) is changed to 1 hour.

Comparative Example 1.1: Preparation of a bionic stomach (no internal biological structure of the stomach)

Based on Example 1.1, the step (2) is changed to: making the kneaded soft clay into a shape of the gastric fundus with a smooth surface to obtain a gastric fundus mold; and the step (4) is changed to: kneading the softened clay The clay body is made into a smooth stomach shape to obtain a stomach body mold. Step (6) is changed to: the kneaded soft clay is made into a smooth gastric antrum shape to obtain a gastric antrum mold. Step (3), (5), (7) The mold baking time is changed to 30 minutes; the mass ratio of the transparent silicone, silicone oil and curing agent in step (8) is changed to 100: 5: 2; The application thickness becomes 2mm.

Comparative Example 1.2: Preparation of a bionic stomach (whole stomach model)

Based on Example 1.1, the steps (2) to (7) are changed to: first make the kneaded soft clay into a smooth stomach shape to obtain a stomach bottom mold, and then place the stomach mold in the oven for baking In 30 minutes, the stomach mold was solidified and formed to cool naturally to obtain a stomach mold; the mass ratio of the transparent silicone, the silicone oil and the curing agent in step (8) was changed to 100: 6: 1; and the thickness of the application in step (10) was changed. It becomes 2mm.

Comparative Example 1.3: Preparation of bionic stomach (silica gel is not evacuated after mixing)

Based on Example 1.1, the mold baking time in steps (3), (5), and (7) was changed to 30 minutes; the mass ratio of transparent silicone, silicone oil, and curing agent in step (8) was changed to 100: 10: 3; omit step (9); change the coating thickness in step (10) to 3 mm; change the standing time in step (11) to 1 hour.

Comparative Example 1.4: Preparation of bionic stomach (increased thickness of smeared silica gel)

Based on Example 1.1, the mold baking time in steps (3), (5), and (7) was changed to 30 minutes; the mass ratio of transparent silicone, silicone oil, and curing agent in step (8) was changed to 100: 8: 2; omit step (9); change the smear thickness in step (10) to 6mm; change the standing time in step (11) to 1 hour.

Comparative Example 1.5: Preparation of bionic stomach (without adding silicone oil)

Based on Example 1.1, the mold baking time in steps (3), (5), and (7) was changed to 30 minutes; the silicone oil in step (8) was omitted, and the transparent silicone and curing agent The mass ratio becomes 100: 3; the smear thickness in step (10) is 2 mm; and the standing time in step (11) is 1 hour.

Comparative Example 1.6: Preparation of bionic stomach (add glycerol)

Based on Example 1.1, the mold baking time in steps (3), (5), and (7) was changed to 30 minutes; the silicone oil in step (8) was replaced with glycerin; and the step (10) The thickness of the coating was changed to 2 mm; the standing time in step (11) was changed to 1 hour.

Example 1.4: Detection of bionic stomach

The human stomach anatomy model, the bionic stomach prepared in Examples 1.1-1.3, the bionic stomach prepared in Comparative Examples 1.1-1.6, and similar foreign products (Fast 10-type silicone bionic stomach purchased from Dragon Skin Company in the United States) were performed in the stomach. The area, food grinding function, back-jet function, transparency, hardness, expansion rate and elasticity are tested. The test results are shown in Table 1.

As can be seen from Table 1, the index comparison between the bionic stomach of the present invention and the real stomach is relatively close, and the index is superior to similar foreign products. Therefore, the bionic stomach of the present invention can replace the real stomach to predict a living test, completely or partially. In vivo tests can achieve the purpose of reducing cost and time, improving repeatability and accuracy, and there is no theoretical limit.

Table 1 Performance test results

Example 2.1: Preparation of bionic large intestine

(1) Preparation of clay clay: repeatedly kneading the soft clay clay to obtain soft clay clay with good ductility;

(2) Large intestine mold compression: Put the kneaded soft clay into the precise human anatomy model of large intestine in medical digestive department, squeeze it hard, remove the human large intestine anatomy model, and obtain the soft clay intestine with the true internal physiological structure of the large intestine. Mold, mold diameter 4cm, length 25cm;

(3) Large intestine mold roasting molding: Put the large intestine mold into the oven at 140 ° C and bake for 15 minutes, solidify the large intestine mold, cool naturally, and obtain a bionic large intestine mold;

(4) Preparation of silica gel: Put transparent silica gel, silicone oil and curing agent ethyl orthosilicate according to 100: 5: 2 in a beaker, stir well and mix well;

(5) Put the evenly mixed silica gel into a vacuum machine, with a negative pressure of 0.09 MPa, and evacuate the foam for 5 minutes to obtain a bubble-free silica gel;

(6) smear the non-bubble silica gel evenly on the surface of the bionic large intestine mold obtained above, with a thickness of about 1 mm;

(7) Allow the uniformly applied large intestine mold to stand for 2 hours to completely solidify and mold;

(8) After the silicone is removed from the large intestine mold, and then subjected to a certain trimming treatment, a bionic large intestine silicone model with intestinal wall folds of the large intestine physiological structure can be obtained.

Example 2.2: Preparation of bionic large intestine

Based on Example 2.1, the mold diameter in step (2) was changed to 4.5 cm; the curing agent in step (4) was replaced with vinyl triamine, and the mass ratio of transparent silicone, silicone oil and curing agent was simultaneously Becomes 100: 4: 1; the thickness of the application in step (6) is changed to 2 mm; and the standing time in step (7) is changed to 4 hours.

Example 2.3: Preparation of bionic large intestine

Based on Example 2.1, the mold diameter in step (2) was changed to 5 cm; the curing agent in step (4) was replaced with diaminodiphenylmethane DDM, and the transparent silicone, silicone oil and curing agent were The mass ratio becomes 100: 6: 3; the smear thickness in step (6) becomes 3 mm.

Comparative Example 2.1: Preparation of bionic large intestine (no wrinkles)

Based on Example 2.1, step (2) is changed to: knead the soft clay clay into a long cylinder with a smooth surface, the mold diameter is 4cm, and the length is 30cm; the baking conditions in step (3) are changed Bake at 130 ° C for 30 minutes; change the mass ratio of transparent silica gel, silicone oil, and curing agent in step (4) to 100: 5: 3; change the coating thickness in step (6) to 2mm; change step (7) The standing time in the middle becomes 1 hour.

Comparative Example 2.2: Preparation of bionic large intestine (silica gel is not evacuated after mixing)

Based on Example 2.1, the mold diameter in step (2) was changed to 4.5 cm; the baking conditions in step (3) were changed to 130 ° C for 30 minutes; and the transparent silica gel in step (4), The mass ratio of the silicone oil and the curing agent becomes 100: 6: 2; step (5) is omitted; and the application thickness in step (6) is changed to 2 mm.

Comparative Example 2.3: Preparation of Bionic Large Intestine

Based on Example 2.1, change the diameter of the mold in step (2) to 5cm; change the baking conditions in step (3) to 130 ° C for 30 minutes; replace the curing agent in step (4) Is a vinyl triamine, and the mass ratio of the transparent silica gel, the silicone oil, and the curing agent is changed to 100: 3: 3; and the coating thickness in step (6) is changed to 8 mm.

Comparative Example 2.4: Preparation of bionic large intestine (without adding silicone oil)

Based on Example 2.1, the diameter of the mold in step (2) was changed to 5 cm; the baking conditions in step (3) were changed to 150 ° C for 15 minutes; and the silicone oil in step (4) was omitted. At the same time, the mass ratio of the transparent silicone curing agent was changed to 100: 3; the thickness of the coating in step (6) was changed to 2 mm.

Comparative Example 2.5: Preparation of bionic large intestine (add glycerol)

Based on Example 2.1, change the diameter of the mold in step (2) to 5cm; change the baking conditions in step (3) to 150 ° C for 15 minutes; replace the silicone oil in step (4) with Glycerin; change the smear thickness in step (6) to 2 mm.

Example 2.4: Detection of bionic large intestine

Anatomical models of human large intestine of the same length, the bionic large intestine prepared in Examples 2.1-2.3, the bionic large intestine prepared in Comparative Examples 2.1-2.5, and similar foreign products (Fast 6-type silicone bionic large intestine purchased from Dragon Skin Company in the United States) The intestinal area, transparency, hardness, expansion rate and elasticity were tested. The test results are shown in Table 2.

It can be known from Table 2 that the index comparison of the bionic large intestine of the present invention is close to that of the real large intestine. Compared with similar foreign products, the index is better. Therefore, the bionic large intestine of the present invention can replace the real large intestine to predict living tests, completely or partially. In vivo tests can achieve the purpose of reducing cost and time, improving repeatability and accuracy, and there is no theoretical limit.

Table 2 Performance test results

Zh Bowel area Transparency effect hardness Expansion rate elasticity Human large intestine anatomy model ＞ 600cm ² opaque 61 30% 420% Example 1 ＞ 600cm ² it is good 60 31% 460% Example 2 ＞ 600cm ² it is good 58 29% 430% Example 3 ＞ 600cm ² it is good 63 31% 470% Comparative Example 1 <200cm ² it is good 64 30% 490% Comparative Example 2 ＞ 600cm ² Poor with bubbles 59 33% 430% Comparative Example 3 ＞ 600cm ² difference 63 28% 420% Comparative Example 4 ＞ 600cm ² it is good 71 10% 520% Comparative Example 5 ＞ 600cm ² it is good 58 20% 550% Foreign similar products <200cm ² it is good 30 10% 1000%

Example 3.1: Preparation of bionic ileum

(1) Preparation of clay clay: repeatedly kneading the soft clay clay to obtain soft clay clay with good ductility;

(2) Ileum mold compression: Put the kneaded soft clay into the accurate human ileum anatomical model in the medical digestive department, squeeze it hard, remove the human ileal anatomical model, and get the soft terra clay ileum with the real internal physiological structure of the ileum. Mold, mold diameter 1.5cm, length 25cm;

(3) Production of hollow fluff: Take a few needles with a diameter of 0.5mm, pierce each mark on the mold, and poke small holes with a diameter of 1mm and a depth of 5mm. The needles will remain inside the mold.

(4) Baking of ileal mold: Put the ileal mold in the oven at 140 ° C for 15 minutes, solidify the ileal mold, cool naturally, and obtain a bionic ileal mold;

(5) Preparation of silica gel: Put transparent silica gel, silicone oil and ethyl orthosilicate according to 100: 3: 1 in a beaker, stir thoroughly and mix well

(6) Put the evenly mixed silica gel into a vacuum machine, with a negative pressure of 0.09 MPa, and evacuate the foam for 5 minutes to obtain a bubble-free silica gel;

(7) Apply the bubble-free silica gel evenly on the surface of the bionic ileal mold obtained above, with a thickness of about 2 mm;

(8) The uniformly applied ileal mold is allowed to stand for 2 hours to completely solidify and mold, and then the needle outside the mold is pulled out to obtain a hollow ileal silica gel model with small intestinal villi;

(9) Cover the surface of the ileal mold without the silicone gel with a piece of oily paper, and place a hollow cylinder with a diameter of 2cm and a height of 4cm on the outside.

(10) Uniformly apply air-free silica gel to the space between the oily paper and the cylinder of the ileum mold, and let it stand for 2 hours until it is completely solidified;

(11) Place the solidified silicone mold in water for 10 minutes, and after the oily paper is softened by water, remove the oily paper from the hollow cylinder;

(12) After removing the silicone from the ileum mold, and then subjecting it to a certain trimming process, a bionic ileal silica gel model with intestinal wall folds and villi, and villi can secrete intestinal fluid can be obtained.

Example 3.2: Preparation of bionic ileum

Based on Example 3.1, the diameter of the mold in step (2) was changed to 2 cm; the diameter of the needle in step (3) was changed to 0.3 mm, and the diameter of the small hole was changed to 0.5 mm; The baking conditions were changed to 150 ° C for 20 minutes; the curing agent in step (5) was changed to vinyltriamine, and the mass ratio of transparent silica gel, silicone oil and curing agent was changed to 100: 3: 1.5; The coating thickness in (7) is 2.5 mm; the standing time in step (8) is 4 hours; and the standing time in step (10) is 1 hour.

Example 3.3: Preparation of bionic ileum

Based on Example 3.1, change the diameter of the mold in step (2) to 2.5 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.1 mm, and the diameter of the small hole to 0.2 mm; Change the baking conditions in step (4) to 130 ° C for 30 minutes; change the curing agent in step (5) to diaminodiphenylmethane DDM, and change the mass ratio of transparent silica gel, silicone oil, and curing agent It is 100: 2.5: 1; the smear thickness in step (7) is changed to 3 mm; the standing time in step (8) is changed to 1 hour; and the standing time in step (10) is changed to 1 hour.

Comparative Example 3.1: Preparation of bionic ileum (no villi)

Based on Example 3.1, change the diameter of the mold in step (2) to 2.5cm and length to 30cm; omit step (3); change the baking conditions in step (4) to 130 ° C for 30 minutes ; Change the mass ratio of transparent silica gel, silicone oil and curing agent in step (5) to 100: 3.5: 0.5; change the thickness of the application in step (7) to 2.5mm; change step (8) to: uniformly apply The ileum mold was left for 1 hour to completely solidify and mold; steps (9)-(11) were omitted.

Comparative Example 3.2: Preparation of bionic ileum (silica gel is not evacuated after mixing)

Based on Example 3.1, change the diameter of the mold in step (2) to 2 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.2 mm and the diameter of the small hole to 0.5 mm; The baking conditions in step (4) were changed to 140 ° C for 20 minutes; the mass ratio of transparent silicone, silicone oil and curing agent in step (5) was changed to 100: 3: 0.5; step (6) was omitted; and step ( The coating thickness in 7) becomes 2.5 mm.

Comparative Example 3.3: Preparation of bionic ileum (increased thickness of smeared silicone)

Based on Example 3.1, change the diameter of the mold in step (2) to 2 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.4 mm and the diameter of the small hole to 0.6 mm; The baking condition in step (4) was changed to 130 ° C for 30 minutes; the mass ratio of transparent silicone, silicone oil and curing agent in step (5) was changed to 100: 2.5: 1.5; and the thickness of the coating in step (7) was changed. It becomes 6mm.

Comparative Example 3.4: Preparation of bionic ileum (without adding silicone oil)

Based on Example 3.1, change the diameter of the mold in step (2) to 2 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.4 mm and the diameter of the small hole to 0.6 mm; The baking conditions in step (4) were changed to 130 ° C for 30 minutes; the silicone oil in step (5) was omitted.

Comparative Example 3.5: Preparation of bionic ileum (add glycerol)

Based on Example 3.1, change the diameter of the mold in step (2) to 2 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.4 mm and the diameter of the small hole to 0.6 mm; The baking conditions in step (4) were changed to 130 ° C for 30 minutes; the silicone oil in step (5) was replaced with glycerin.

Example 3.4: Detection of bionic ileum

Anatomical models of human ileum of the same length, the bionic ileum prepared in Examples 3.1-3.3, the bionic ileum prepared in Comparative Examples 3.1-3.5, and similar foreign products (Fast Model 3 silicone bionic ileum purchased from Dragon Skin Company in the United States) The intestinal area, transparency, hardness, swelling rate, elasticity test and villi function test were performed. The test results are shown in Table 3.

As can be seen from Table 3, the index comparison between the bionic ileum of the present invention and the real ileum is relatively close, and the index is superior to similar foreign products. Therefore, the bionic ileum of the present invention can replace the real ileum to predict living tests, completely or partially. In vivo tests can achieve the purpose of reducing cost and time, improving repeatability and accuracy, and there is no theoretical limit.

Table 3 Performance test results

Example 4.1: Preparation of bionic duodenum

(1) Preparation of clay clay: repeatedly kneading the soft clay clay to obtain soft clay clay with good ductility;

(2) Duodenum mold compression: Put the kneaded soft clay into a precise human duodenum anatomical model in the medical digestive department, squeeze it hard, and remove the human duodenal anatomical model to get a real Soft clay mud duodenum mold with internal physiological structure of duodenum, mold diameter 3cm, length 25cm;

(3) Production of hollow fluff: Take a few needles with a diameter of 0.5mm, pierce each mark on the mold, and poke small holes with a diameter of 1mm and a depth of 5mm. The needles will remain inside the mold.

(4) Duodenal mold roasting and molding: Put the duodenal mold in the oven at 140 ° C for 15 minutes, solidify the duodenal mold, cool naturally, and obtain a bionic duodenal mold;

(5) Preparation of silica gel: Put transparent silica gel, silicone oil and curing agent ethyl orthosilicate according to 100: 1: 3 in a beaker, stir thoroughly and mix well;

(6) Put the evenly mixed silica gel into a vacuum machine, with a negative pressure of 0.09 MPa, and evacuate the foam for 5 minutes to obtain a bubble-free silica gel;

(7) Apply the bubble-free silica gel evenly on the surface of the bionic duodenum mold obtained above, with a thickness of about 1 mm;

(8) The duodenum mold evenly applied is left for 2 hours to completely solidify, and then the needle outside the mold is pulled out to obtain a hollow duodenum silicone model with small intestinal villi;

(9) Cover the surface of the duodenum mold without the silicone with a needle, and cover it with a piece of oily paper, and place a hollow cylinder with a diameter of 2cm and a height of 4cm on the outside and fix it well;

(10) Apply even air-free silica gel to the space between the oily paper of the duodenum mold and the cylinder, and let it stand for 2 hours until it is completely solidified;

(11) Place the solidified silicone mold in water for 10 minutes. After the oily paper is softened by water, remove the oily paper from the hollow cylinder;

(12) After removing the duodenum mold from the silicone, and then subjecting it to a certain trimming process, a bionic duodenum silicone model with intestinal wall folds and villi, which can secrete intestinal fluid, can be obtained. .

Example 4.2: Preparation of bionic duodenum

Based on Example 4.1, the mold diameter in step (2) was changed to 3.5 cm; the diameter of the needle in step (3) was changed to 0.3 mm, and the diameter of the small hole was changed to 0.5 mm; and step (4) The baking conditions were changed to 150 ° C for 20 minutes; the curing agent in step (5) was changed to vinyltriamine, and the mass ratio of transparent silica gel, silicone oil and curing agent was changed to 100: 1.5: 3; The thickness of the application in step (7) is 1.5 mm; the standing time in step (8) is 4 hours; and the standing time in step (10) is 1 hour.

Example 4.3: Preparation of bionic duodenum

Based on Example 4.1, change the diameter of the mold in step (2) to 4 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.1 mm, and the diameter of the small hole to 0.2 mm; The baking conditions in step (4) were changed to 130 ° C for 30 minutes; the curing agent in step (5) was changed to diaminodiphenylmethane DDM, and the mass ratio of transparent silicone, silicone oil and curing agent was changed to 100: 1: 2.5; change the smear thickness in step (7) to 2mm; change the rest time in step (8) to 1 hour; change the rest time in step (10) to 1 hour.

Comparative Example 4.1: Preparation of bionic duodenum (no villi)

Based on Example 4.1, the mold length in step (2) was changed to 30 cm; step (3) was omitted; the baking conditions in step (4) were changed to 130 ° C for 30 minutes; and step (5) The mass ratio of medium transparent silicone, silicone oil and curing agent becomes 100: 1: 3; the thickness of the application in step (7) becomes 1.5mm; and the step (8) becomes: the ileal mold applied evenly is allowed to stand 1 Hours, it is completely solidified; steps (9)-(11) are omitted.

Comparative Example 4.2: Preparation of bionic duodenum (silica gel is not evacuated after mixing)

Based on Example 4.1, change the diameter of the mold in step (2) to 3.5 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.2 mm, and the diameter of the small hole to 0.5 mm; Change the baking conditions in step (4) to 140 ° C for 20 minutes; change the mass ratio of transparent silica gel, silicone oil to curing agent in step (5) to 100: 0.5: 2.5; omit step (6); change step The coating thickness in (7) becomes 1.5 mm.

Comparative Example 4.3: Preparation of bionic duodenum (increased thickness of smeared silica gel)

Based on Example 4.1, change the diameter of the mold in step (2) to 4 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.4 mm and the diameter of the small hole to 0.6 mm; The baking condition in step (4) was changed to 130 ° C for 30 minutes; the mass ratio of transparent silicone, silicone oil and curing agent in step (5) was changed to 100: 1.5: 3.5; and the coating thickness in step (7) was changed. It becomes 6mm.

Comparative Example 4.4: Preparation of bionic duodenum (without adding silicone oil)

Based on Example 4.1, change the diameter of the mold in step (2) to 4 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.4 mm and the diameter of the small hole to 0.6 mm; The baking conditions in step (4) were changed to 130 ° C for 30 minutes; the silicone oil in step (5) was omitted.

Comparative example 4.5: Preparation of bionic duodenum (add glycerol)

Based on Example 4.1, change the diameter of the mold in step (2) to 4 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.4 mm and the diameter of the small hole to 0.6 mm; The baking conditions in step (4) were changed to 130 ° C for 30 minutes; the silicone oil in step (5) was replaced with glycerin.

Example 4.4: Detection of bionic duodenum

An anatomy model of human duodenum of the same length, a bionic duodenum prepared in Examples 4.1-4.3, a bionic duodenum prepared in Comparative Examples 4.1-4.5, and similar foreign products (purchased from Dragon Skin Company, USA) Fast bionic duodenum of type 8) was tested for intestinal area, transparency, hardness, expansion rate, elasticity and villous function. The test results are shown in Table 4.

It can be known from Table 4 that the index of the bionic duodenum of the present invention is close to that of the real duodenum. Compared with similar foreign products, the index is better. Therefore, the bionic duodenum of the present invention can replace the real duodenum. The intestine is used to predict a living test, completely or partially replacing a living test, to achieve the purpose of reducing costs and time, improving repeatability and accuracy, and there is no theoretical limit.

Table 4 Performance test results

Example 5.1: Preparation of bionic jejunum

(1) Preparation of clay clay: repeatedly kneading the soft clay clay to obtain soft clay clay with good ductility;

(2) Jejunum mold compression: Put the kneaded soft clay into the precise human jejunum anatomical model of the medical digestive department, and squeeze it vigorously to remove the human jejunum anatomical model to obtain the soft ceramic mud jejunum with the internal physiological structure of the real jejunum Mold, mold diameter 2cm, length 25cm;

(3) Production of hollow fluff: Take a few needles with a diameter of 0.5mm, pierce each mark on the mold, and poke small holes with a diameter of 1mm and a depth of 5mm. The needles will remain inside the mold.

(4) Baking the jejunum mold: Put the jejunum mold in the oven at 140 ° C for 15 minutes, solidify the jejunum mold, cool naturally, and obtain a bionic jejunum mold;

(5) Preparation of silica gel: Put transparent silica gel, silicone oil and curing agent ethyl orthosilicate according to 100: 2: 2 in a beaker, stir well and mix well;

(6) Put the evenly mixed silica gel into a vacuum machine, with a negative pressure of 0.09 MPa, and evacuate the foam for 5 minutes to obtain a bubble-free silica gel;

(7) Apply the bubble-free silica gel evenly on the surface of the bionic jejunum mold obtained above, with a thickness of about 1.5 mm;

(8) The uniformly applied jejunum mold is allowed to stand for 2 hours to completely solidify, and then the needle outside the mold is pulled out to obtain a hollow jejunal silica gel model with small intestinal villi;

(9) Cover the surface of the jejunum mold without silicone with a piece of oily paper with the needle just pulled out, and place a hollow cylinder with a diameter of 2cm and a height of 4cm on the outside and fix it well;

(10) Uniformly apply air-free silica gel to the space between the oily paper and the cylinder of the jejunum mold, and let it stand for 2 hours until it is completely solidified;

(11) Place the solidified silicone mold in water for 10 minutes, and after the oily paper is softened by water, remove the oily paper from the hollow cylinder;

(12) After the silica gel is removed from the jejunum mold, and after a certain trimming treatment, a bionic jejunum silica gel model with jejunal physiological structure intestinal wall folds and villi, and villi can secrete intestinal fluid can be obtained.

Example 5.2: Preparation of bionic jejunum

Based on Example 5.1, change the diameter of the mold in step (2) to 2.5 cm; change the diameter of the needle in step (3) to 0.3 mm, and the diameter of the small hole to 0.5 mm; change step (4) The baking conditions were changed to 150 ° C for 20 minutes; the curing agent in step (5) was changed to vinyltriamine, and the mass ratio of transparent silicone, silicone oil and curing agent was changed to 100: 1.5: 2; The thickness of the application in step (7) was changed to 2 mm; the standing time in step (8) was changed to 4 hours; and the standing time in step (10) was changed to 1 hour.

Example 5.3: Preparation of bionic jejunum

Based on Example 5.1, change the diameter of the mold in step (2) to 3 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.1 mm and the diameter of the small hole to 0.2 mm; The baking conditions in step (4) were changed to 130 ° C for 30 minutes; the curing agent in step (5) was changed to diaminodiphenylmethane DDM, and the mass ratio of transparent silicone, silicone oil and curing agent was changed to 100: 2: 2.5; change the smear thickness in step (7) to 2.5 mm; change the rest time in step (8) to 1 hour; change the rest time in step (10) to 1 hour.

Comparative Example 5.1: Preparation of bionic jejunum (no fluff)

Based on Example 5.1, change the diameter of the mold in step (2) to 2.5cm and length to 30cm; omit step (3); change the baking conditions in step (4) to 130 ° C for 30 minutes ; Change the mass ratio of transparent silicone, silicone oil and curing agent in step (5) to 100: 1.5: 2.5; change the thickness of the application in step (7) to 2mm; change step (8) to: uniformly apply The ileum mold was allowed to stand for 1 hour to completely solidify and mold; steps (9)-(11) were omitted.

Comparative Example 5.2: Preparation of bionic jejunum (silica gel is not evacuated after mixing)

Based on Example 5.1, change the diameter of the mold in step (2) to 2.5 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.2 mm, and the diameter of the small hole to 0.5 mm; Change the baking conditions in step (4) to 140 ° C for 20 minutes; change the mass ratio of transparent silicone, silicone oil and curing agent in step (5) to 100: 2.5: 1.5; omit step (6); change step The coating thickness in (7) becomes 2 mm.

Comparative Example 5.3: Preparation of bionic jejunum (increase the thickness of silicone gel)

Based on Example 5.1, change the diameter of the mold in step (2) to 2.5 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.4 mm, and the diameter of the small hole to 0.6 mm; Change the baking conditions in step (4) to 130 ° C for 30 minutes; change the mass ratio of transparent silicone, silicone oil, and curing agent in step (5) to 100: 2: 2; and apply the coating in step (7) The thickness becomes 6 mm.

Comparative Example 5.4: Preparation of bionic jejunum (without adding silicone oil)

Based on Example 5.1, change the diameter of the mold in step (2) to 2.5 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.4 mm, and the diameter of the small hole to 0.6 mm; The baking condition in step (4) was changed to 130 ° C for 30 minutes; the silicone oil in step (5) was omitted.

Comparative Example 5.5: Preparation of bionic jejunum (add glycerol)

Based on Example 5.1, change the diameter of the mold in step (2) to 2.5 cm and length to 30 cm; change the diameter of the needle in step (3) to 0.4 mm, and the diameter of the small hole to 0.6 mm; The baking conditions in step (4) were changed to 130 ° C for 30 minutes; the silicone oil in step (5) was replaced with glycerin.

Example 5.4: Detection of bionic jejunum

Anatomical models of human jejunum of the same length, the bionic jejunum prepared in Examples 5.1-5.3, the bionic jejunum prepared in Comparative Examples 5.1-5.5, and similar foreign products (Fast Model 4 silicone bionic jejunum purchased from Dragon Skin Company in the United States) The intestinal area, transparency, hardness, swelling rate, elasticity test and villous function test were performed. The test results are shown in Table 5.

It can be known from Table 5 that the index comparison between the bionic jejunum and the jejunum of the present invention is relatively close, and the index is superior to similar foreign products. Therefore, the bionic jejunum of the present invention can replace the real jejunum to predict a living test, completely or partially Instead of living test, the purpose of reducing cost and time, improving repeatability and accuracy is achieved, and there is no theoretical limit.

Table 5 Performance test results

Although the present invention has been disclosed as above with the preferred embodiments, it is not intended to limit the present invention. Any person familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. The protection scope of the present invention shall be defined by the claims.

Claims (18)

A bionic digestive tract, wherein the components of the bionic digestive tract include a substrate and an auxiliary material; the substrate includes one or more of silica gel, latex, or hydrogel; and the auxiliary material includes silicone oil And curing agents; The digestive tract contains one or more of the stomach, large intestine, ileum, duodenum, and jejunum; When the digestive tract is the stomach, the mass ratio between the substrate, the silicone oil, and the curing agent is 100: (5 to 10): (1 to 3); or when the digestive tract is the large intestine, the substrate, silicone oil, and curing The mass ratio between the agents is 100: (3 to 6): (1 to 3); or when the digestive tract is the ileum, the mass ratio between the substrate, the silicone oil, and the curing agent is 100: (2.5 to 3.5) : (0.5 to 1.5); or when the digestive tract is duodenum, the mass ratio between the substrate, the silicone oil and the curing agent is 100: (0.5 to 1.5): (2.5 to 3.5); or the digestion When the tract is a jejunum, the mass ratio between the substrate, the silicone oil, and the curing agent is 100: (1.5 to 2.5): (1.5 to 2.5). The bionic digestive tract according to claim 1, wherein the substrate is silica gel. The bionic digestive tract according to claim 2, wherein the silica gel is transparent silica gel or translucent silica gel. The bionic digestive tract according to any one of claims 1 to 3, wherein the curing agent comprises ethyl orthosilicate, vinyltriamine, DETA aminoethylpiperazine AE, m-phenylenediamine m -One or more of PDAMPD, diaminodiphenylmethane DDM or HT-972DEH-50. The bionic digestive tract according to any one of claims 1 to 4, wherein when the digestive tract is a stomach, a method for preparing a bionic stomach is to first prepare a physiological structure with a true gastric fundus, a gastric body, and an internal antrum of the stomach Of the gastric fundus, gastric body and gastric antrum mold, and then apply the bionic gastric raw material on the surface of the gastric fundus, gastric body and gastric antrum mold according to a certain thickness and leave it to rest, so that the biomimetic gastric raw material is solidified and formed, and the mold is removed to obtain bionic Stomach; of which, the gastric fundus, gastric body and gastric antrum molds with the actual physiological structure of the gastric fundus, gastric body, and internal antrum are prepared. Plastic materials are placed in and filled with the accurate gastric anatomical model of the human body, Stomach body and gastric antrum, then remove the accurate human stomach anatomy model from Medical Gastroenterology Department and solidify the plastic material to obtain the gastric fundus, gastric body and gastric antrum mold with real gastric fundus, gastric body and internal structure of gastric antrum; The bionic gastric material is obtained by mixing a base material and an auxiliary material for making a bionic stomach according to a certain mass ratio, and putting the bionic gastric material in a vacuum machine to evacuate the foam; When the digestive tract is a large intestine, a method for preparing a bionic large intestine is to first prepare a large intestine mold having a true internal physiological structure of the large intestine, and then apply the bionic large intestine raw material on the surface of the large intestine mold and leave it in a certain thickness to solidify the bionic large intestine Forming and removing the mold to obtain a bionic large intestine; of which, a large intestine mold having a real internal physiological structure of the large intestine is prepared by placing a plastic material and filling the large intestine of a precise human anatomy model of the medical digestive department, and then removing the accurate human body of the medical digestive department The large intestine of the intestinal anatomical model and the plastic material are cured and shaped to obtain a large intestine mold with the true internal physiological structure of the large intestine; the bionic large intestine material is a mixture of a base material and an auxiliary material for making a bionic large intestine according to a certain quality ratio Evacuated in a vacuum machine; When the digestive tract is the ileum, a method for preparing a bionic ileum is to first prepare an ileal mold having a real internal physiological structure of the ileum and retaining needles, and then apply the bionic ileal raw material to the ileal mold and the human ileal anatomical model according to a certain thickness. The contacting side is left to stand, so that the bionic ileal raw material is solidified and formed. The needle is pulled out and the mold is removed to obtain a hollow bionic ileum with small intestinal villi and a hollow; in which, a real ileal internal physiological structure is prepared and the needle is retained. The ileal mold of the object is filled with a plastic material and filled with an accurate anatomical model of the human ileum in the medical digestive department. Then, the ileal anatomical model of the human body is removed, and then the depressions corresponding to the small intestine villi on the human ileal anatomical model Use a needle to poke out a small hole, and finally keep the needle on the plastic material and solidify and mold the plastic material to obtain an ileal mold that has the actual internal ileum internal physiological structure and retains the needle; the bionic ileum The material is based on a certain quality ratio. After the materials are mixed, they are put into a vacuum machine and evacuated. Or when the digestive tract is the ileum, a method for preparing a bionic ileum is to first prepare an ileal mold having a true internal physiological structure of the ileum and retaining needles, and then apply the bionic ileal raw material to the ileal mold and human ileum anatomy according to a certain thickness. The contacting side of the model is left to stand, so that the bionic ileal raw material is solidified and formed. The needle is pulled out without taking off the mold to obtain a hollow bionic ileum with small intestinal villi and a hollow bionic ileum. Cover that side with a layer of spacer, and apply the bionic ileal material on the side that does not contact the bionic ileum according to a certain thickness and let it stand, so that the bionic ileal material applied on the surface of the spacer solidifies and removes the isolation. And remove the mold to obtain a bionic ileum with physiological structure of the ileum and secretion of intestinal fluid. Among them, an ileum mold having a true internal ileum physiological structure and retaining needles is prepared by placing plastic materials and filling it with medical digestion Accurate human ileal anatomical model, then remove the human ileal anatomical model, and then Each depression of the plastic material corresponding to the small intestinal villi on the anatomical model of the human ileum is punctured with small needles. Finally, the needles are kept on the plastic material and the plastic material is solidified to obtain a true ileum. The internal ileum mold with an internal physiological structure and a needle-shaped object is retained; the bionic ileal material is obtained by mixing a base material and an auxiliary material for making a bionic ileum according to a certain mass ratio and putting them in a vacuum machine to evacuate and evacuate; When the digestive tract is the duodenum, a method for preparing a bionic duodenum is to first prepare an ileum mold having a true internal physiological structure of the duodenum and retaining needles, and then bionic the duodenum according to a certain thickness The duodenal raw material is smeared on the side where the duodenal mold is in contact with the human duodenal anatomical model and left to stand, so that the bionic duodenal raw material is solidified and formed. The needle is pulled out and the mold is removed to obtain a small intestine. Villous and hollow bionic duodenum; in which, a duodenum mold having a true internal physiological structure of the duodenum and retaining needles is prepared, and a plastic material is put into and filled with a precise human body in the medical digestive department. Duodenum anatomical model, then remove the human duodenum anatomical model, and then poke small holes with needles in the depressions corresponding to the small intestinal villi on the human duodenal anatomical model. The needles are kept on the plastic material and the plastic material is cured and formed to obtain a duodenum mold having a real internal duodenal physiological structure and retaining the needles. The duodenum biomimetic material is prepared after mixing ratio bionic duodenum substrate in accordance with certain auxiliary mass and placed in a vacuum machine vacuum bubble row obtained; Or when the digestive tract is the duodenum, the method for preparing the bionic duodenum is to first prepare an ileum mold that has the true internal physiological structure of the duodenum and retains needles, and then bionic ten in accordance with a certain thickness The duodenal raw material is smeared on the side of the duodenal mold that is in contact with the human duodenal anatomical model and left to stand, so that the bionic duodenal raw material is solidified and formed. The needle is pulled out without removing the mold to obtain Hollow bionic duodenum with small intestinal villi, and then covering the bionic duodenum with a layer of spacers on the side that does not contact the duodenal mold, and bionic duodenal raw materials according to a certain thickness Apply on the side where the spacer is not in contact with the bionic duodenum and let it stand, so that the raw material of the bionic duodenum applied on the surface of the spacer is solidified and formed, the spacer is removed and the mold is removed, and the duodenum is obtained. Intestinal physiological structure is villi and can secrete intestinal fluid. The bionic duodenum is prepared. The ileum mold with the actual internal duodenal internal physiological structure and retaining needles is prepared. Full Medicine Gastroenterology Department accurate human duodenum anatomical model, then remove the human duodenum anatomical model, and then use needle-shaped depressions in each of the plastic materials corresponding to the small intestinal villi on the human duodenal anatomical model The object poke out a small hole, and finally the needles are kept on the plastic material and the plastic material is cured and formed to obtain a duodenum mold having a true internal duodenal physiological structure and retaining the needles; said The bionic duodenal material is obtained by mixing the base material and auxiliary materials for making the bionic duodenum according to a certain mass ratio, and then putting the material into a vacuum machine to evacuate the bubbles; When the digestive tract is a jejunum, a method for preparing a bionic jejunum is to first prepare a jejunum mold having a real internal physiological structure of the jejunum and retaining needles, and then apply the bionic jejunum raw material on the jejunum mold and the human jejunum anatomical model according to a certain thickness. The contacted side is left to stand to solidify the bionic jejunum raw material, pull out the needle and remove the mold to obtain a hollow bionic jejunum with small intestinal villi and a hollow; in which, a real jejunum internal physiological structure is prepared and the needle is retained The jejunum mold of the object is a plastic material placed in and filled with a precise human jejunum anatomical model in medical digestive medicine, and then the human jejunum anatomical model is removed, and then each of the plastic material is corresponding to the depression of the small intestine villi on the human jejunum anatomical model. Use a needle to poke out a small hole, and finally keep the needle on the plastic material and solidify the plastic material to obtain a jejunum mold that has the actual physiological structure of the jejunum and retains the needle; the bionic jejunum The material is based on a certain quality ratio. After mixing into a vacuum machine vacuum bubble row obtained; Or when the digestive tract is the jejunum, the method for preparing the bionic jejunum is to first prepare a jejunum mold that has the actual physiological structure of the jejunum and retains needles, and then apply the bionic jejunum raw material on the jejunum mold and the human jejunum anatomy according to a certain thickness The contacting side of the model was left to stand to solidify the raw material of the bionic jejunum. The needles were pulled out without taking off the mold to obtain a hollow bionic jejunum with small intestinal villi and a hollow bionic jejunum. Cover that side with a layer of spacer, and apply the bionic jejunum raw material on the side that does not contact the bionic jejunum and leave it in a certain thickness, so that the bionic jejunal raw material applied on the surface of the spacer is solidified to form the isolation. And remove the mold to obtain a bionic jejunum with a jejunum physiological structure and secretion of intestinal fluid. Among them, a jejunum mold having a real jejunum internal physiological structure and retaining needles is prepared by placing a plastic material and filling it with medical digestion Accurate human jejunum anatomical model, then remove the human jejunum anatomical model, and then Use a needle to poke out a small hole in each depression of the plastic material that corresponds to the small intestine villi on the anatomical model of the human jejunum. Finally, keep the needle on the plastic material and solidify the plastic material to obtain a true The jejunum mold with the internal structure of the jejunum and retaining needles; the bionic jejunum material is obtained by mixing the base material and auxiliary materials for making the bionic jejunum according to a certain mass ratio, and putting the material into a vacuum machine to evacuate and evacuate. A method for preparing a bionic stomach, which is characterized in that the method firstly prepares the bottom, stomach, and antrum molds with the actual physiological structure of the bottom, stomach, and internal structure of the antrum, and then the bionic stomach is formed according to a certain thickness. The raw material is smeared on the surface of the gastric fundus, gastric body, and antrum mold and left to stand, so that the bionic gastric raw material is solidified and formed, and the mold is removed to obtain a biomimetic stomach. The gastric fundus, gastric body and gastric antrum molds are filled with plastic materials and filled with the accurate human gastric anatomical model of the medical digestive department. The plastic material is cured and molded to obtain a gastric fundus, gastric body and gastric antrum mold having a real physiological structure of the gastric fundus, gastric body, and internal antrum of the stomach; the bionic gastric material is based on a certain mass ratio, The auxiliary materials are mixed and put into a vacuum machine to evacuate the foam; the substrate comprises one or more of silica gel, latex or hydrogel; the auxiliary materials And a curing agent comprising a silicone oil. The bionic stomach prepared by applying the method of claim 6. A method for preparing a bionic large intestine, which is characterized in that the method firstly prepares a large intestine mold having a true internal physiological structure of the large intestine, and then applies the bionic large intestine raw material on the surface of the large intestine mold according to a certain thickness and leaves it to stand, so that the bionic large intestine The raw materials are solidified and formed, and the mold is removed to obtain a bionic large intestine. Among them, a large intestine mold having a true internal physiological structure of the large intestine is prepared by placing and filling a plastic material into a large intestine of a precise human anatomy model of the medical digestive department, and then removing the medical digestive department. Accurate human intestinal anatomical model of the large intestine and solidifying the plastic material to obtain a large intestine mold with a true internal physiological structure of the large intestine; the bionic large intestine material is obtained by mixing a base material and an auxiliary material for making a bionic large intestine according to a certain mass ratio It is obtained by putting into a vacuum machine to evacuate the bubble; the substrate includes one or more of silica gel, latex, or hydrogel; and the auxiliary material includes silicone oil and a curing agent. The bionic large intestine prepared by applying the method of claim 8. A method for preparing a bionic ileum, which is characterized in that the method firstly prepares an ileal mold having a real internal physiological structure of the ileum and retains needles, and then applies the bionic ileal raw material to the ileal mold and the human ileum according to a certain thickness. The side where the anatomical model is in contact is left to stand, so that the raw material of the bionic ileum is solidified and formed. The needle is pulled out and the mold is removed to obtain a hollow bionic ileum with small intestinal villi and a hollow body. Among them, a real ileal internal physiological structure is prepared and retained Needle-shaped ileum molds are filled with plastic materials and filled with accurate anatomical models of human ileum in medical digestive department, and then the human ileal anatomical models are removed, and each of the plastic materials corresponds to the small intestine villi on the human ileal anatomical model. A small hole is pierced with a needle in the depression of the recess, and finally the needle is kept on the plastic material and the plastic material is cured and molded to obtain an ileal mold having a true internal ileum internal physiological structure and retaining the needle; Bionic ileal material is the basis for making bionic ileum according to a certain mass ratio. The materials and auxiliary materials are mixed and put into a vacuum machine to evacuate the foam; Or the method is to first prepare an ileal mold with a real internal ileal internal physiological structure and retain needles, and then apply the bionic ileal raw material on the side of the ileal mold that is in contact with the human ileal anatomical model according to a certain thickness and let it stand, The bionic ileum raw material is solidified and formed, and the needle is pulled out without taking off the mold to obtain a hollow bionic ileum with small intestinal villi, and then covering the side of the bionic ileum that is not in contact with the ileal mold. The bionic ileal raw material is applied to the side of the separator that does not contact the bionic ileum with a certain thickness and left to stand, so that the bionic ileal raw material applied on the surface of the separator is solidified and formed, and the separator is removed and the mold is removed to obtain a ileal physiology. Bionic ileum with a structure of villi and secretion of intestinal fluid; among which, an ileum mold having a real internal ileum internal physiological structure and retaining needles is prepared, and a plastic material is put in and filled with a precise human ileal anatomical model in the medical digestive department, and then removed Human ileum anatomical model, then in each of the plastic materials with the human ileum On the cutaway model, the small intestine corresponding to the villi of the small intestine punctures small holes with needles. Finally, the needles are kept on the plastic material and the plastic material is cured and shaped to obtain a true ileal internal physiological structure and retain the needle shape. The ileal mold of the object; the bionic ileal material is obtained by mixing the base material and the auxiliary material for making the bionic ileum according to a certain mass ratio and putting it in a vacuum machine to evacuate the foam; The substrate includes one or more of silica gel, latex, or hydrogel; and the auxiliary material includes silicone oil and / or a curing agent. The bionic ileum prepared by applying the method of claim 10. A method for preparing bionic duodenum, which is characterized in that the method firstly prepares an ileum mold having a true internal physiological structure of the duodenum and retaining needles, and then bionic duodenum according to a certain thickness The intestinal raw material is smeared on the side where the duodenal mold is in contact with the human duodenal anatomical model and left to stand, so that the bionic duodenal raw material is solidified and formed. The needle is pulled out and the mold is removed to obtain the intestinal villi. And it is a hollow bionic duodenum; in which, a duodenum mold having a real internal physiological structure of the duodenum and retaining needles is prepared, and a plastic material is put in and filled with a precise human twelve in the medical digestive department. Anatomy model of the duodenum, then remove the anatomical model of the duodenum of the human body, and then use a needle to poke out a small hole in each depression of the plastic material corresponding to the small intestine villi on the human duodenal anatomical model. The needles continue to be retained on the plastic material and the plastic material is cured and formed to obtain a duodenal mold having a true internal physiological structure of the duodenum and retaining the needles. ; After the biomimetic material is a duodenum according to a certain ratio of the mass production base bionic duodenum were mixed and placed in a vacuum auxiliary machine vacuum bubble row obtained; Or the method is to first prepare an ileum mold with the actual internal physiological structure of the duodenum and retain needles, and then apply the bionic duodenal raw material to the duodenum mold and the human duodenum according to a certain thickness. The intestinal anatomical model is in contact with the side and allowed to stand, so that the bionic duodenal raw material is solidified and formed. The needles are pulled out without taking off the mold to obtain a hollow bionic duodenum with small intestinal villi and hollow. The side of the duodenum that is not in contact with the duodenal mold is covered with a layer of spacer, and the bionic duodenal raw material is smeared on the side that does not contact the bionic duodenum according to a certain thickness. The bionic duodenum raw material applied on the surface of the spacer is solidified and formed, and the spacer is removed and the mold is removed to obtain a bionic duodenum with duodenal physiological structure villi and secretion of intestinal fluid; Among them, a duodenum mold having a true internal physiological structure of the duodenum and retaining needles is prepared by filling and filling a plastic material with accurate human duodenum anatomy in the medical digestive department Model, then remove the human duodenal anatomical model, and then poke small holes with needles in the depressions corresponding to the small intestinal villi on the human duodenal anatomical model, and finally pierce the needles Continue to remain on the plastic material and solidify and mold the plastic material to obtain an ileum mold that has the true internal physiological structure of the duodenum and retains needles; the bionic duodenal material is made according to a certain quality ratio The base material and auxiliary materials of the bionic duodenum are mixed and put into a vacuum machine to evacuate and evacuate; The substrate includes one or more of silica gel, latex, or hydrogel; and the auxiliary material includes silicone oil and / or a curing agent. The bionic duodenum prepared by applying the method of claim 12. A method for preparing a bionic jejunum is characterized in that the method firstly prepares a jejunum mold having a real internal structure of the jejunum and retains needles, and then applies the bionic jejunum raw material on the jejunum mold and the human jejunum according to a certain thickness. The contacting side of the anatomical model is left to stand to solidify the raw material of the bionic jejunum. The needle is pulled out and the mold is removed to obtain a hollow bionic jejunum with small intestinal villi and hollow; among which, the real jejunum internal physiological structure is prepared and retained. Needle-shaped jejunum mold is to put plastic material into and fill the precise human jejunum anatomical model of medical digestive department, then remove the human jejunum anatomical model, and then each of the plastic material corresponds to the small intestine villi on the human jejunum anatomical model A small hole is pierced with a needle in the depression of the hollow, and finally the needle is kept on the plastic material and the plastic material is cured and formed to obtain a jejunum mold having a real jejunum internal physiological structure and retaining the needle; The bionic jejunum material is based on a certain quality ratio. After mixing and put in a vacuum auxiliary machine vacuum bubble row obtained; Or the method is to first prepare a jejunum mold that has the actual physiological structure of the jejunum and retains needles, and then apply the bionic jejunum raw material on the side of the jejunum mold that is in contact with the jejunum anatomical model of the human body according to a certain thickness and let it stand. The bionic jejunum raw material is solidified and shaped, the needle is pulled out without taking off the mold, and a bionic jejunum with small intestinal villi and hollow is obtained, and then the side of the bionic jejunum that is not in contact with the jejunum mold is covered with a layer of spacers. The bionic jejunum raw material is applied to the side where the spacer is not in contact with the bionic jejunum with a certain thickness and left to stand, so that the bionic jejunal raw material applied on the surface of the spacer is solidified and formed, the spacer is removed, and the mold is removed to obtain a jejunum physiology A bionic jejunum with a structure of villi and secretion of intestinal fluid. Among them, a jejunum mold having a real physiological structure of the jejunum and retaining needles is prepared, and a plastic material is put in and filled with a precise human jejunum anatomical model of the medical digestive department, and then removed Human jejunum anatomical model, and then in each of the plastic materials with the human jejunum On the cutaway model, the small intestine corresponding to the villi of the small intestine punctures small holes with needles. Finally, the needles are kept on the plastic material and the plastic material is cured and shaped to obtain a true jejunum internal physiological structure and retain the needle shape. The jejunum mold of the object; the bionic jejunum material is obtained by mixing the base material and the auxiliary material for making the bionic jejunum according to a certain mass ratio and putting it in a vacuum machine to evacuate the bubble; The substrate includes one or more of silica gel, latex, or hydrogel; and the auxiliary material includes silicone oil and / or a curing agent. The bionic jejunum prepared by applying the method of claim 14. A bionic material, characterized in that the components of the bionic material include a substrate and an auxiliary material; the substrate includes one or more of silica gel, latex, or hydrogel; the auxiliary material includes silicone oil and curing Agent. The biomimetic material according to claim 16, wherein the mass ratio between the substrate, the silicone oil, and the curing agent is 100: (0.5 to 10): (0.5 to 3.5). The bionic digestive tract according to any one of claims 1 to 5 or the preparation method according to claim 6 or the bionic stomach according to claim 7 or the preparation method according to claim 8 or the bionic large intestine according to claim 9 or The preparation method according to claim 10 or the bionic ileum according to claim 11 or the preparation method according to claim 12 or the bionic duodenum according to claim 13 or the preparation method or claim 14 Application of the bionic jejunum of 15 or the bionic material of claim 16 or 17 in simulating the actual digestive system of a human body.

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