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WO2019233456A1 - Inhibiteur d'erk et son utilisation - Google Patents

Inhibiteur d'erk et son utilisation Download PDF

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Publication number
WO2019233456A1
WO2019233456A1 PCT/CN2019/090220 CN2019090220W WO2019233456A1 WO 2019233456 A1 WO2019233456 A1 WO 2019233456A1 CN 2019090220 W CN2019090220 W CN 2019090220W WO 2019233456 A1 WO2019233456 A1 WO 2019233456A1
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WO
WIPO (PCT)
Prior art keywords
pyridin
chloro
methyl
hydroxymethyl
pyrazine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2019/090220
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English (en)
Chinese (zh)
Inventor
吴颢
唐晓婧
谷伟
吴文茂
沈益飞
杨晓峰
王维
湛波
赵新涛
冯东杰
陈忠研
柯永新
兰宏
王家炳
丁列明
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Betta Pharmaceuticals Co Ltd
Original Assignee
Betta Pharmaceuticals Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Betta Pharmaceuticals Co Ltd filed Critical Betta Pharmaceuticals Co Ltd
Priority to CN201980031575.0A priority Critical patent/CN112204027A/zh
Publication of WO2019233456A1 publication Critical patent/WO2019233456A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A61K31/415—1,2-Diazoles
    • A61K31/4162—1,2-Diazoles condensed with heterocyclic ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/4985—Pyrazines or piperazines ortho- or peri-condensed with heterocyclic ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/55—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole
    • A61K31/551—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole having two nitrogen atoms, e.g. dilazep
    • A61K31/5513—1,4-Benzodiazepines, e.g. diazepam or clozapine
    • A61K31/5517—1,4-Benzodiazepines, e.g. diazepam or clozapine condensed with five-membered rings having nitrogen as a ring hetero atom, e.g. imidazobenzodiazepines, triazolam
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/06—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom containing only hydrogen and carbon atoms in addition to the ring nitrogen atom
    • C07D213/16—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom containing only hydrogen and carbon atoms in addition to the ring nitrogen atom containing only one pyridine ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D498/12—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains three hetero rings

Definitions

  • the invention relates to a series of compounds as extracellular signal-regulated kinase (ERK) inhibitors, a preparation method thereof, and a pharmaceutical composition.
  • ERK extracellular signal-regulated kinase
  • the present invention also relates to the use of the above compound or a pharmaceutical composition thereof in the treatment of ERK-mediated diseases.
  • MNK mitogen-activated protein kinase
  • Extracellular signal-regulated kinase is a member of the MAPK family. Its signal transmission pathway is the core of the signal network involved in regulating cell growth, development, and division. From extracellular stimulation to cells, to Corresponding biological effects of cells must pass through the three-stage kinase cascade of the MAPK signal transduction pathway, that is, upstream activation protein ⁇ MAPK kinase kinase (MAPKKK) ⁇ MAPK kinase (MAP-KK) ⁇ MAPK.
  • MAPKKK upstream activation protein
  • MAPKKK MAPK kinase kinase
  • MAPK-KK MAPK kinase
  • Ras acts as an upstream activator protein
  • Raf acts as MAPKKK
  • MAPK / ERK kinase (MEK) acts as MAPKK
  • ERK is MAPK
  • Ras-Raf-MEK-ERK pathway MAPKKK phosphorylates MAPKK's serine and threonine sites and activates them, which in turn causes MAPKK to phosphorylate threonine and serine sites of MAPK.
  • Phosphorylated activated ERK1 / 2 is translocated from the cytoplasm into the nucleus, which then mediates the activation of multiple transcription factors and genes such as Elk-1, ATF, NF- ⁇ B, Ap-1, c-fos, and c-Jun And transcription, participate in a variety of biological responses such as cell proliferation and differentiation, maintenance of cell morphology, cytoskeleton construction, apoptosis and canceration of cells.
  • ERK includes ERK1 and ERK2 and is the key to transmitting signals from surface receptors to the nucleus.
  • the MAPK kinases ERK1 and ERK2 are widely expressed and participate in the RAS-RAF-MEK-ERK signaling cascade. They both contain unique N- and C-termini that provide signal specificity.
  • the kinase domain also contains a residue of 31 amino acids, making It is functionally specific.
  • multiple mitogens or other stimuli can activate multiple subtypes of RAS (HRAS, NRAS, and KRAS), and activated RAS can recruit and activate various RAF subunits (including ARAF, BRAF, and CRAF)
  • RAS RAS
  • the cascade activates MEK1 / 2, mediates the phosphorylation of downstream ERK1 and ERK2, thereby activating ERK1 / 2, regulates the activation and transcription of hundreds of its cytoplasmic and nuclear substrates, and the occurrence of related biological effects.
  • RAF subunits including ARAF, BRAF, and CRAF
  • the RAS-RAF-MEK-ERK signaling cascade plays a key role in the occurrence and development of various diseases, including brain injury, cancer, cardiac hypertrophy, diabetes, and inflammation. Especially in cancer, about 98% of pancreatic cancer, 52% of colorectal cancer, and 32% of lung adenocarcinoma have KRAS mutations, and 28% of melanomas have NRAS gene mutations. In addition, about 40-60% of melanomas, 40% of thyroid cancers and 20% of colorectal cancers have BRAF mutations (see Vakiani E, solit DB. KRAS and BrAF; KRAS and BRAF; drug targets and predictive biomarkers; Journal of Pathology 2011, 223, 219-29).
  • ERK inhibitors currently in clinical development include BVD-523, GDC-0994, KO-947, LY-3214996, and LTT462, etc., but they are all in the early stage of clinical development of Phase I / II. There are currently no drugs on the market and they need to be developed. Effective ERK inhibitor.
  • ERK inhibitor described in the present invention should be understood as being capable of inhibiting ERK1 and / or ERK2.
  • the present invention relates to a compound as an inhibitor of extracellular signal-regulated kinase (ERK), or a pharmaceutically acceptable salt, solvate, chelate, non-covalent complex or prodrug thereof.
  • ERK extracellular signal-regulated kinase
  • the structure of the compound of the present invention is represented by formula (I):
  • X or Y is arbitrarily selected from N or CH;
  • Z or U is arbitrarily selected from N or CR 10 , and R 10 is arbitrarily selected from H or unsubstituted or optionally substituted C 1-8 alkyl;
  • V is arbitrarily selected from O or S;
  • R 1 and R 2 together with the C atom to which they are attached form a C 3-10 cycloalkyl or C 3-10 heterocyclyl, said C 3-10 cycloalkyl or C 3-10 heterocyclyl being unsubstituted or Optionally substituted with halogen, hydroxy, oxo, C 1-8 alkyl or C 1-8 alkyl-C 5-10 aryl;
  • R 3 is arbitrarily selected from H, halogen, hydroxyl, CN, C 1-8 alkyl, C 1-8 alkoxy, C 2-8 alkenyl, or C 2-8 alkynyl, said C 1-8 alkynyl Group, C 1-8 alkoxy, C 2-8 alkenyl or C 2-8 alkynyl is unsubstituted or optionally substituted with halogen, hydroxyl or C 1-8 alkyl;
  • R 5 is arbitrarily selected from H, halogen, hydroxyl, CN, C 1-8 alkyl, C 1-8 alkoxy, C 2-8 alkenyl, C 2-8 alkynyl, C 3-10 cycloalkyl , C 6-10 aryl, C 5-10 heteroaryl or C 3-10 heterocyclyl, said C 1-8 alkyl, C 1-8 alkoxy, C 2-8 alkenyl, C 2 -8 alkynyl, C 3-10 cycloalkyl, C 6-10 aryl, C 5-10 heteroaryl or C 3-10 heterocyclyl is unsubstituted or optionally substituted with halogen, hydroxyl or C 1-8 alkane Radical substitution
  • R 6 is optionally selected from halogen, hydroxy, amino, CN, C 1-8 alkyl or NR 7 R 8 ;
  • R 9 is arbitrarily selected from H, halogen, hydroxy, C 1-8 alkyl or C 1-8 alkoxy, said C 1-8 alkyl or C 1-8 alkoxy being unsubstituted or optionally halogen , Hydroxy or C 1-8 alkyl substitution;
  • heterocyclic or heteroaryl ring group optionally contains 1, 2 or 3 heteroatoms independently selected from N, O or S;
  • n is arbitrarily selected from 0, 1 or 2.
  • X and Y in formula (I) are both N.
  • Z in formula (I) is N, U is CH or Z is CH, and U is N.
  • both Z and U in formula (I) are CH.
  • V in Formula (I) is O.
  • R 1 in formula (I) is H
  • R 1 in formula (I) is H
  • R 1 in formula (I) is H
  • R 2 is
  • R 1 or R 2 in formula (I) is arbitrarily selected from halogen, hydroxyl, C 1-6 alkyl, C 1-6 alkoxy, -C 1-6 alkylhydroxy, -C 1-6 alkyl-C 1-6 alkoxy or halogen-substituted C 1-6 alkyl.
  • R 1 or R 2 in formula (I) is arbitrarily selected from F, Cl, hydroxy, methyl, methoxy, hydroxy-substituted methyl, methoxy-substituted methyl, or halogen-substituted methyl.
  • R 1 and R 2 in formula (I) together with the C atom to which they are attached form the following substituents:
  • R 3 in formula (I) is arbitrarily selected from H, hydroxy, halogen, or -C 1-6 alkyl-hydroxy.
  • R 3 in formula (I) is selected from H.
  • R 5 in formula (I) is arbitrarily selected from H, halogen, hydroxyl, amino, CN, or C 1-6 alkyl, said C 1-6 alkyl being unsubstituted or optionally substituted with halogen .
  • R 5 in formula (I) is arbitrarily selected from H, F, Cl, hydroxyl, amino, CN or C 1-3 alkyl, said C 1-3 alkyl being unsubstituted or arbitrarily Halogen substitution.
  • R 5 in formula (I) is H, F, Cl, -CF 3 , CN, or methyl.
  • R 6 in formula (I) is arbitrarily selected from halogen, hydroxy, C 1-6 alkyl, or NR 7 R 8 .
  • R 6 in formula (I) is NR 7 R 8
  • R 7 or R 8 is arbitrarily selected from H, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, Hydroxyl-substituted isobutyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, hydroxyl-substituted cyclohexyl, acetyl,
  • R 6 in formula (I) is NR 7 R 8 , R 7 is H, and R 8 is isopropyl or
  • R 9 is H or F.
  • n or n in formula (I) is arbitrarily selected from the following group:
  • the invention further provides a compound or a pharmaceutically acceptable salt thereof, wherein the compound is selected from:
  • the present invention also provides a pharmaceutical composition comprising an effective therapeutic dose of at least any one compound of the formula (I) of the present invention and at least one pharmaceutically acceptable excipient.
  • the invention further provides a pharmaceutical composition, wherein the weight ratio of the compound represented by the structural formula (I) to the excipient is 0.0001-10.
  • the invention provides an application of a compound or a pharmaceutical composition represented by the structural formula (I) in the preparation of a medicament.
  • said application is to treat, prevent, delay or prevent the occurrence or progression of cancer or cancer metastasis.
  • the application is to prepare a medicament for treating a disease mediated by ERK.
  • the disease is cancer.
  • the cancer is selected from breast cancer, multiple myeloma, bladder cancer, endometrial cancer, gastric cancer, cervical cancer, rhabdomyosarcoma, non-small cell lung cancer, small cell lung cancer, polymorphous lung cancer, ovarian cancer, and esophagus Cancer, melanoma, colorectal cancer, hepatocellular carcinoma, head and neck cancer, hepatobiliary cell carcinoma, myelodysplastic syndrome, malignant glioma, prostate cancer, thyroid cancer, Schwann cell tumor, lung squamous cell Cancer, lichenoid keratosis, synovial sarcoma, skin cancer, pancreatic cancer, testicular cancer, or liposarcoma.
  • said application is as an ERK inhibitor.
  • said use is as an ERK1 and / or ERK2 inhibitor.
  • the present invention also provides a method of administering a therapeutically effective amount of a compound or a pharmaceutical composition of at least any one of the formula (I) to a subject to treat and / or prevent a disease mediated by ERK.
  • the ERK includes ERK1 and / or ERK2.
  • the ERK-mediated disease is cancer.
  • the cancer is selected from the group consisting of breast cancer, multiple myeloma, bladder cancer, endometrial cancer, gastric cancer, cervical cancer, rhabdomyosarcoma, non-small cell lung cancer, small cell lung cancer, and pleomorphism.
  • Lung cancer ovarian cancer, esophageal cancer, melanoma, colorectal cancer, hepatocellular carcinoma, head and neck tumors, hepatobiliary cell carcinoma, myelodysplastic syndrome, malignant glioma, prostate cancer, thyroid cancer, Schwann cells Tumor, lung squamous cell carcinoma, lichenoid keratosis, synovial sarcoma, skin cancer, pancreatic cancer, testicular cancer, liposarcoma.
  • the present invention also provides a method for treating cancer, which comprises administering to a subject a therapeutically effective amount of at least any one compound represented by formula (I) or a pharmaceutical composition or a pharmaceutical composition, said cancer being breast cancer, multiple Myeloma, bladder cancer, endometrial cancer, gastric cancer, cervical cancer, rhabdomyosarcoma, non-small cell lung cancer, small cell lung cancer, pleomorphic lung cancer, ovarian cancer, esophageal cancer, melanoma, colorectal cancer, hepatocellular carcinoma, Head and neck cancer, hepatobiliary cell carcinoma, myelodysplastic syndrome, malignant glioma, prostate cancer, thyroid cancer, Schwann cell tumor, lung squamous cell carcinoma, lichenoid keratosis, synovial sarcoma, skin Cancer, pancreatic cancer, testicular cancer, or liposarcoma.
  • the subject to be treated is a human.
  • halogen means fluorine, chlorine, bromine or iodine.
  • Preferred halogen groups include fluorine, chlorine and bromine.
  • alkyl includes straight-chain or branched monovalent saturated hydrocarbon groups.
  • alkyl includes methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, 3- (2-methyl) butyl, 2 -Pentyl, 2-methylbutyl, neopentyl, n-hexyl, 2-hexyl, 2-methylpentyl and the like.
  • C 1-8 alkyl means comprising 7 or 8 carbon atoms, a straight-chain or branched-chain arranged in the form of Group.
  • Alkenyl and alkynyl include straight or branched chain alkenyl and alkynyl.
  • C 2-8 alkenyl and C 2-8 alkynyl refer to alkenyl groups containing 2, 3, 4, 5, 6, 7, or 8 carbon atoms arranged in a straight or branched chain. Or alkynyl.
  • Alkoxy refers to the oxyether form of the aforementioned straight or branched chain alkyl group, ie, -O-alkyl.
  • compositions comprising "a” pharmaceutically acceptable excipient can be interpreted to mean that the composition includes “one or more” pharmaceutically acceptable excipients.
  • aryl refers to an unsubstituted or substituted monocyclic or fused ring aromatic group including a carbon ring atom, unless otherwise specified.
  • Preferred aryl groups are 6 to 10 membered monocyclic or bicyclic aromatic ring groups.
  • Preferred are phenyl and naphthyl. Most preferred is phenyl.
  • heterocyclyl refers to an unsubstituted or substituted 3-8 membered stable monocyclic ring consisting of carbon atoms and 1-3 heteroatoms selected from N, O or S A system in which nitrogen or sulfur heteroatoms can be selectively oxidized and nitrogen heteroatoms can be selectively quaternized.
  • the heterocyclic group may be attached to any heteroatom or carbon atom to form a stable structure.
  • heterocyclic groups include, but are not limited to, azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, oxopiperazinyl, oxopiperidinyl, tetrahydrofuranyl, dioxolane, Tetrahydroimidazolyl, tetrahydrothiazolyl, tetrahydrooxazolyl, tetrahydropyranyl, morpholinyl, thiomorpholinyl, thiomorpholinyl sulfoxide, thiomorpholinyl sulfone, and tetrahydro Oxadiazolyl.
  • heteroaryl refers to an unsubstituted or substituted stable 5- or 6-membered monocyclic aromatic ring system or an unsubstituted or substituted 9- or 10-membered benzocondensate Heteroaromatic ring system or bicyclic heteroaromatic ring system consisting of carbon atoms and 1-4 heteroatoms selected from N, O or S, and wherein said nitrogen or sulfur heteroatoms can be selectively oxidized The nitrogen heteroatom may be selectively quaternized.
  • Heteroaryl groups can be attached to any heteroatom or carbon atom to form a stable structure.
  • heteroaryl groups include, but are not limited to, thienyl, furyl, imidazolyl, isoxazolyl, oxazolyl, pyrazolyl, pyrrolyl, thiazolyl, thiadiazolyl, triazolyl, pyridyl, pyridyl Azinyl, indolyl, azaindolyl, indazolyl, benzimidazolyl, benzofuranyl, benzothienyl, benzoisoxazolyl, benzothiazolyl, benzothiazolyl, benzene Thiathiazolyl, benzotriazolyl adenine, quinolyl or isoquinolyl.
  • cycloalkyl refers to a cyclic saturated alkyl chain having 3 to 10 carbon atoms, for example, cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl.
  • substituted means that one or more hydrogen atoms in the group are replaced with the same or different substituents, respectively.
  • the substituent is independently selected from the group consisting of -F, -Cl, -Br, -I, -OH, trifluoromethoxy, ethoxy, propoxy, isopropoxy, n-butoxy Group, isobutoxy, tert-butoxy, -SCH 3 , -SC 2 H 5 , formaldehyde, -C (OCH 3 ), cyano, nitro, -CF 3 , -OCF 3 , amino, dimethyl Amino, methylthio, sulfonyl, and acetyl groups.
  • substituted alkyl examples include, but are not limited to, 2-aminoethyl, 2-hydroxyethyl, pentachloroethyl, trifluoromethyl, methoxymethyl, pentafluoroethyl, and piperazinylmethyl.
  • substituted alkoxy examples include, but are not limited to, aminomethoxy, trifluoromethoxy, 2-diethylaminoethoxy, 2-ethoxycarbonylethoxy, 3-hydroxypropoxy.
  • pharmaceutically acceptable salt refers to a salt prepared from a pharmaceutically acceptable non-toxic base or acid.
  • pharmaceutically acceptable non-toxic bases including inorganic bases and organic bases.
  • Salts derived from inorganic bases include salts such as aluminum, ammonium, calcium, copper (high and low prices), ferric, ferrous, lithium, magnesium, manganese (high and low prices), potassium, sodium, zinc and the like. Particularly preferred are the salts of ammonium, calcium, magnesium, potassium and sodium.
  • Non-toxic organic bases capable of deriving into salts include primary, secondary and tertiary amines, as well as cyclic amines and amines containing substituents, such as naturally occurring and synthetic amines containing substituents.
  • non-toxic organic bases capable of forming salts include ion exchange resins and arginine, betaine, caffeine, choline, N ′, N′-dibenzylethylenediamine, diethylamine, 2 -Diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, reduced glucosamine, glucosamine, histidine, halamine, isopropylamine , Lysine, methylglucosamine, morpholine, piperazine, piperidine, polyamine resin, procaine, purine, theobromine, triethylamine, trimethylamine, tripropylamine, tromethamine and the like.
  • the corresponding salt can be conveniently prepared from pharmaceutically acceptable non-toxic acids, including inorganic acids and organic acids.
  • acids include, for example, acetic acid, benzenesulfonic acid, benzoic acid, camphorsulfonic acid, citric acid, ethanesulfonic acid, isethionic acid, formic acid, fumaric acid, gluconic acid, glutamic acid, hydrobromic acid, Hydroiodic acid, perchloric acid, hydrochloric acid, isethionic acid, propionic acid, glycolic acid, lactic acid, maleic acid, malic acid, mandelic acid, methanesulfonic acid, mucic acid, nitric acid, oxalic acid, acetic acid, pantothenic acid, phosphoric acid , Succinic acid, sulfuric acid, 2-naphthalenesulfonic acid, cyclohexylaminesulfonic acid, salicylic acid, saccharin
  • the compound represented by formula (I) will be used as a medicine, it is preferable to use a certain purity, for example, at least 60% purity, a more suitable purity is at least 75%, and a particularly suitable purity is at least 98% (% is weight ratio).
  • Prodrugs of the compounds of the invention are included within the scope of the invention.
  • the prodrug refers to a functional derivative that is easily converted into the desired compound in vivo.
  • any pharmaceutically acceptable salt, ester, salt of an ester, or other derivative of a compound of the present application which is capable of directly or indirectly providing the compound of the present application or a pharmaceutically active metabolite thereof, or Residues.
  • Particularly preferred derivatives or prodrugs are those compounds that, when administered to a patient, can increase the bioavailability of the compounds of the present application (e.g., can make the oral compound more easily absorbed into the blood), or promote the parent compound to a biological organ or Those compounds delivered by the site of action, such as the brain or lymphatic system.
  • the term "administration" in the treatment method provided by the present invention refers to the administration of a compound disclosed in the present invention that can treat different diseases, or, although not explicitly disclosed, can be converted into the present disclosure in vivo after administration to a subject Compound of compounds.
  • Conventional methods for selecting and preparing suitable prodrug derivatives have been described in books such as Design of Prodrugs (ed. H. Bundgaard, Elsevier, 1985).
  • the compounds according to the invention may contain one or more asymmetric centers and may result in diastereomers and optical isomers.
  • the invention includes all possible diastereomers and their racemic mixtures, their substantially pure resolved enantiomers, all possible geometric isomers, and their pharmaceutically acceptable salts.
  • the above formula (I) does not precisely define the stereo structure of a certain position of the compound.
  • the present invention includes all stereoisomers of the compound represented by formula (I) and pharmaceutically acceptable salts thereof. Further, mixtures of stereoisomers and specific stereoisomers isolated are also included in the present invention. In the synthesis of such compounds, or in the process of racemization or epimerization known to those skilled in the art, the product obtained may be a mixture of stereoisomers.
  • the present invention includes any possible tautomer and a pharmaceutically acceptable salt thereof, and mixtures thereof.
  • the present invention includes any possible solvate and polymorph.
  • the type of the solvate-forming solvent is not particularly limited as long as the solvent is pharmaceutically acceptable.
  • water, ethanol, propanol, acetone and the like can be used.
  • composition refers to a product including a specified amount of each specified ingredient, and any product produced directly or indirectly from a combination of specified amounts of each specified ingredient. Therefore, a pharmaceutical composition containing the compound of the present invention as an active ingredient and a method for preparing the compound of the present invention are also part of the present invention.
  • some crystalline forms of the compound may exist in a polymorphic form, and this polymorphic form is included in the present invention.
  • some compounds can form solvates with water (ie, hydrates) or common organic solvents, and such solvates also fall within the scope of the present invention.
  • the pharmaceutical composition provided by the present invention comprises, as an active ingredient, a compound represented by formula (I) (or a pharmaceutically acceptable salt thereof), a pharmaceutically acceptable excipient, and other optional therapeutic components or Excipients.
  • a pharmaceutical composition of the present invention includes Pharmaceutical composition for parenteral (including subcutaneous, intramuscular, intravenous) administration.
  • the pharmaceutical composition of the present invention can be conveniently prepared in the unit dosage form known in the art and any preparation method known in the pharmaceutical field.
  • the compound represented by formula (I), or a prodrug, or a metabolite, or a pharmaceutically acceptable salt of the present invention can be mixed with a drug carrier to form a drug combination as an active ingredient.
  • a drug carrier can take a variety of forms, depending on the mode of administration desired, for example, oral or injection (including intravenous). Therefore, the pharmaceutical composition of the present invention can be used as a separate unit suitable for oral administration, such as a capsule, cachet, or tablet containing a predetermined dose of the active ingredient.
  • the pharmaceutical composition of the present invention may be in the form of a powder, granule, solution, aqueous suspension, non-aqueous liquid, oil-in-water emulsion, or water-in-oil emulsion.
  • the compound represented by formula (I) or a pharmaceutically acceptable salt thereof may also be administered by a controlled release manner and / or a delivery device.
  • the pharmaceutical composition of the present invention can be prepared by any pharmaceutical method. Generally, this method includes the step of associating the active ingredient with a carrier that makes up one or more of the necessary ingredients.
  • the pharmaceutical composition is prepared by uniform and close mixing of the active ingredient with a liquid carrier or a finely divided solid carrier or a mixture of the two.
  • the product can be easily prepared into a desired appearance.
  • the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier and a compound represented by formula (I) or a stereoisomer, tautomer, polymorph, solvate, pharmaceutically acceptable Salt, its prodrug. Combinations of a compound represented by formula (I) or a pharmaceutically acceptable salt thereof with one or more other compounds having therapeutic activity are also included in the pharmaceutical composition of the present invention.
  • the pharmaceutical carrier used in the present invention may be, for example, a solid carrier, a liquid carrier, or a gas carrier.
  • Solid carriers include, but are not limited to, lactose, gypsum powder, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, and stearic acid.
  • Liquid carriers include, but are not limited to, syrup, peanut oil, olive oil, and water.
  • Gas carriers including but not limited to carbon dioxide and nitrogen.
  • water, ethylene glycol, oils, alcohols, flavor enhancers, preservatives, colorants and the like can be used for oral liquid preparations such as suspensions, elixirs and solutions; and carriers such as starch, sugars, Microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents and the like can be used for oral solid preparations such as powders, capsules and tablets.
  • oral solid preparations such as powders, capsules and tablets.
  • the tablet coating may use standard aqueous or non-aqueous formulation techniques.
  • the tablets containing the compound or pharmaceutical composition of the present invention can be formed by compression or molding, and optionally, can be made into tablets together with one or more auxiliary components or adjuvants.
  • the active ingredients are mixed in a free-flowing form, such as powder or granules, with a binder, lubricant, inert diluent, surfactant or dispersant, and compressed tablets can be made by compression in a suitable machine.
  • a powdered compound or pharmaceutical composition is wetted with an inert liquid diluent and then molded in a suitable machine by molding.
  • each tablet contains about 0.05 mg to 5 g of active ingredient
  • each cachet or capsule contains about 0.05 mg to 5 g of active ingredient.
  • a formulation intended for oral administration in humans contains from about 0.5 mg to about 5 g of active ingredient, compounded with a suitable and conveniently metered auxiliary material, the auxiliary material accounting for about 5% to 95% of the total pharmaceutical composition.
  • Unit dosage forms generally contain about 1 mg to about 2 g of active ingredient, typically 25 mg, 50 mg, 100 mg, 200 mg, 300 mg, 400 mg, 500 mg, 600 mg, 800 mg, or 1000 mg.
  • the pharmaceutical composition suitable for parenteral administration provided by the present invention can be prepared by adding the active ingredient to water to prepare an aqueous solution or suspension.
  • a suitable surfactant such as hydroxypropyl cellulose may be included.
  • Dispersions can also be made in glycerol, liquid polyethylene glycols, and their mixtures in oil. Further, a preservative may be included in the pharmaceutical composition of the present invention to prevent the growth of harmful microorganisms.
  • the invention provides pharmaceutical compositions suitable for injection, including sterile aqueous solutions or dispersions.
  • the above pharmaceutical composition can be prepared into a sterile powder form for instant preparation of a sterile injection solution or dispersion.
  • the final injection form must be sterile and, for easy injection, it must be easy to flow.
  • the pharmaceutical composition must be stable during preparation and storage. Therefore, preferably, the pharmaceutical composition is stored under conditions that are resistant to contamination by microorganisms such as bacteria and fungi.
  • the carrier can be a solvent or dispersion medium, for example, water, ethanol, polyol (such as glycerol, propylene glycol, and liquid polyethylene glycol), vegetable oils, and suitable mixtures thereof.
  • the pharmaceutical composition provided by the present invention may be in a form suitable for topical application, for example, an aerosol, an emulsion, an ointment, a lotion, dusting powder, or other similar dosage forms. Further, the pharmaceutical composition provided by the present invention may be in a form suitable for use in a transdermal drug delivery device.
  • These preparations can be prepared by a conventional processing method using the compound represented by formula (I) of the present invention, or a pharmaceutically acceptable salt thereof.
  • an emulsion or ointment is prepared by adding about 5 to 10% by weight of a hydrophilic material and water to produce an emulsion or ointment having a desired consistency.
  • the pharmaceutical composition provided by the present invention can be in a form suitable for rectal administration with a solid as a carrier.
  • Unit-dose suppositories are the most typical dosage form. Suitable excipients include cocoa butter and other materials commonly used in the art. Suppositories can be conveniently prepared by first mixing the pharmaceutical composition with softened or melted excipients, then cooling and mold forming.
  • the above formulation may also include, as appropriate, one or more additional excipient components such as a diluent, a buffer, a flavoring agent, a binder, a surfactant, a Thickeners, lubricants and preservatives (including antioxidants).
  • additional excipient components such as a diluent, a buffer, a flavoring agent, a binder, a surfactant, a Thickeners, lubricants and preservatives (including antioxidants).
  • other adjuvants may also include penetration enhancers that regulate the isotonic pressure of the drug and blood.
  • a pharmaceutical composition containing a compound represented by formula (I), or a pharmaceutically acceptable salt thereof can be prepared in the form of a powder or a concentrated solution.
  • the dosage level of the drug is about 0.01 mg / kg body weight to 150 mg / kg body weight per day, or 0.5 mg to 7 g per patient per day.
  • inflammation, cancer, psoriasis, allergies / asthma, diseases and discomforts of the immune system, diseases and discomforts of the central nervous system (CNS) effective dosages of drugs for treatment from 0.01 mg / kg body weight to 50 mg / kg body weight per day, or 0.5mg to 3.5g per patient per day.
  • ATP adenosine triphosphate
  • BINAP binaphthyl diphenylphosphine
  • Boc tert-butoxycarbonyl
  • Bpd bis (pinacol) diboron
  • DBU 1,8-diazabicycloundec-7-ene
  • DIAD diisopropyl azodicarboxylate
  • DIBAl-H diisobutylaluminum hydride
  • DIEA or DIPEA N, N-diisopropylethylamine
  • DME ethylene glycol dimethyl ether
  • DMSO dimethyl sulfoxide
  • EA ethyl acrylate
  • EDCI.HCl 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride
  • HATU 2- (7-benzotriazole) -N, N, N ′, N′-tetramethylurea hexafluorophosphate
  • HOBt 1-hydroxybenzotriazole
  • iprMgCl isopropyl magnesium chloride
  • LAH lithium tetrahydroaluminum
  • MeMgBr methyl magnesium bromide
  • MOMCl chloromethyl methyl ether
  • NEt 3 triethylamine
  • NMM N-methylmorpholine
  • PBD 2- (4-biphenyl) -5-phenyloxadiazole
  • PCC pyridinium chlorochromate
  • Pd 2 dba 3 or Pd 2 (dba) 3 tris (dibenzylideneacetone) dipalladium;
  • Pd (dppf) Cl 2 1,1′-bisdiphenylphosphinoferrocene palladium dichloride
  • Pd (PPh 3 ) 4 tetrakis (triphenylphosphine) palladium
  • Pd (PPh 3 ) 2 Cl 2 dichlorobis (triphenylphosphine) palladium;
  • PE petroleum ether
  • Pin 2 B 2 pinacol biborate
  • TBAI tetrabutylammonium iodide
  • TBDPSCl tert-butyldiphenylchlorosilane
  • TBSCl tert-butyldimethylchlorosilane
  • TFA trifluoroacetic acid
  • TMSCF 2 Br triethyl (bromodifluoromethyl) silane
  • xantphos 4,5-bisdiphenylphosphine-9,9-dimethylxanthene
  • XPhos or Xphos 2-dicyclohexylphosphine-2,4,6-triisopropylbiphenyl.
  • the filter cake Transfer to a 100 mL Erlenmeyer flask, add 10 mL of methanol to dissolve, dry with anhydrous sodium sulfate, filter, and concentrate under reduced pressure to obtain 0.700 g of off-white solid. That is M1.
  • Example 1 Compound 1 (8- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (3-chlorobenzyl) -4,4-difluoro-2,3 , 4,5-Tetrahydro-1H-pyrrolo [1,2-a] [1,4] diazalide-1-one)
  • Example 2 Compound 2 (7- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (3-chlorobenzyl) -3- (hydroxymethyl) -3, Preparation of 4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one)
  • Example 3 Compound 3 (8 ′-(5-chloro-2- (isopropylamino) pyridin-4-yl) -2 ′-(2- (hydroxymethyl) benzyl) -2 ′, 3 ′ -Dihydro-1′H, 5′H-spiro [oxetane-3,4′-pyrrolo [1,2-a] [1,4] diazepine] -1′-one) Preparation
  • Example 4 Compound 4 (8 ′-(5-chloro-2- (isopropylamino) pyridin-4-yl) -2 ′-(3-chlorobenzyl) -2 ′, 3′-dihydro- Preparation of 1′H, 5′H-spiro [azetidin-3,4′-pyrrolo [1,2-a] [1,4] diazalide] -1′-one)
  • Example 5 Compound 5 (8- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (3-chlorobenzyl) -4,4-bis (hydroxymethyl) -2,3,4,5-tetrahydro-1H-pyrrolo [1,2-a] [1,4] diazalide-1-one)
  • Example 6 Compound 6 (2 ′-(5-chloro-2- (isopropylamino) pyridin-4-yl) -5 ′-(2- (hydroxymethyl) benzyl) -5 ′, 6, -Dihydro-4′H, 8′H-spiro [oxetane-3,7′-pyrazolo [1,5-a] [1,4] diazepine] -4′-one ) Preparation
  • Example 7 Compound 7 (2- (5-chloro-2- (isopropylamino) pyridin-4-yl) -7- (chloromethyl) -7- (hydroxymethyl) -5- (2- Preparation of (hydroxymethyl) benzyl) -5,6,7,8-tetrahydro-4H-pyrazolo [1,5-a] [1,4] diazalide-4-one)
  • Example 8 Compound 8 (2- (5-chloro-2- (isopropylamino) pyridin-4-yl) -8- (3-chlorobenzyl) -7,8-dihydro-5H, 9H- Preparation of spiro [imidazo [1,2-a] [1,4] diazepine-6,3′-oxetane] -9-one)
  • Example 9 Compound 9 (8 ′-(5-chloro-2- (isopropylamino) pyridin-4-yl) -2 ′-(3-chlorobenzyl) -1- (2,4-dimethyl (Oxybenzyl) -2 ', 3'-dihydro-1'H, 5'H-spiro [azetidin-3,4'-pyrrolo [1,2-a] [1,4] Preparation of diazepine] -1′-one)
  • Example 10 Compound 10 ((S) -8 ′-(5-chloro-2- (isopropylamino) pyridin-4-yl) -2 ′-(1- (3-chlorophenyl) -2- (Hydroxyethyl) -2 ′, 3′-dihydro-1′H, 5′H-spiro [oxetane-3,4′-pyrrolo [1,2-a] [1,4] di Preparation of azepine] -1′-one)
  • Example 35 Compound 35 (8 ′-(5-chloro-2- (isopropylamino) pyridin-4-yl) -2 ′-(2- (hydroxymethyl) benzyl) -1-methyl- 2 ′, 3′-dihydro-1′H, 5′H-spiro [azetidine-3,4′-pyrrolo [1,2-a] [1,4] diazepine] -1 ′ -Keto) Preparation
  • Example 36 Compound 36 ((R) -7- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (5-fluoro-2- (hydroxymethyl) benzyl) Preparation of 3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one)
  • Example 37 Compound 37 (8- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (5-fluoro-2- (hydroxymethyl) benzyl) -4- ( (Hydroxymethyl) -2,3,4,5-tetrahydro-1H-pyrrolo [1,2-a] [1,4] diazalide-1-one)
  • Example 38 Compound 38 (8- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (5-fluoro-2- (hydroxymethyl) benzyl) -2,3 -Dihydro-1H, 5H-spiro [pyrrolo [1,2-a] [1,4] diazepine-4,5 '-[1,3] dioxane] -1-one)
  • Example 39 Compound 39 (8- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (5-fluoro-2- (hydroxymethyl) benzyl) -4,4 -Preparation of bis (methoxymethyl) -2,3,4,5-tetrahydro-1H-pyrrolo [1,2-a] [1,4] diazalide-1-one)
  • Example 40 Compound 40 ((R) -7- (5-chloro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2- (5- Preparation of fluoro-2- (hydroxymethyl) benzyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one)
  • the organic phases were separated, dried over anhydrous sodium sulfate, filtered, and concentrated. 20.0g of crude.
  • the crude product was pulped with petroleum ether to methyl tert-butyl ether (4: 1) in volume ratio to obtain 14.7 g of pure solid.
  • the product was 18.9 g, which was compound 36j.
  • reaction solution was filtered through diatomaceous earth, the filter cake was washed with ethyl acetate (100mL * 3), and the filtrate was washed with water (100mL * 3).
  • the organic phase was separated, dried, concentrated, and passed through a column (first with pure The dichloromethane was washed away, and then petroleum ether to ethyl acetate (2: 1 to 1: 2) was used to obtain 16.5 g of the product, which is compound 40e.
  • Example 41 Compound 41 ((R) -2- (5-chloro-2- (isopropylamino) pyridin-4-yl) -7- (5-fluoro-2- (hydroxymethyl) benzyl) -6- (methoxymethyl) -6,7-dihydroimidazo [1,2-a] pyrazine-8- (5H) -one)
  • the compound 41 was synthesized following the procedure for synthesizing similar compounds in the compound of the example.
  • Example 42 Compound 42 ((R) -7- (5-chloro-3-fluoro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2 -(5-fluoro-2- (hydroxymethyl) benzyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H)- Ketone)
  • Example 43 Compound 43 ((3R) -7- (5-chloro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2- (5- Fluoro-2- (1-hydroxyethyl) benzyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one) Preparation
  • Example 44 Compound 44 ((R) -7- (5-chloro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2- (5- Fluoro-2- (2-hydroxypropyl-2-yl) benzyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H ) -Keto) Preparation
  • Example 45 Compound 45 ((R) -7- (5-chloro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2- (5- Preparation of fluoro-2- (fluoromethyl) benzyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one)
  • Example 46 Compound 46 ((R) -7- (5-chloro-2-((4-fluoro-1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl)- 2- (5-fluoro-2- (hydroxymethyl) benzyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -Keto) Preparation
  • Compound 46 was synthesized by following the procedures for synthesizing similar compounds in the examples.
  • Example 47 Compound 47 ((R) -7- (5-chloro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2- (3- Preparation of hydroxyphenyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one)
  • Example 48 Compound 48 ((R) -7- (5-chloro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2- (2- Preparation of hydroxyphenyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one)
  • Compound 48 was synthesized according to the above synthetic route with reference to the synthetic steps of similar compounds in the compounds of the examples.
  • Example 49 Compound 49 ((R) -7- (5-chloro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2-((2 -Hydroxypyridin-3-yl) methyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one)
  • Compound 49e was synthesized according to the above synthetic route with reference to the synthetic steps of similar compounds in the compound of the example.
  • Example 50 Compound 50 ((R) -7- (5-chloro-2- (isopropylamino) pyridin-4-yl) -3- (methoxymethyl) -2- (2-(( Preparation of methylsulfonyl) methyl) benzyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one)
  • Example 51 Compound 51 (8- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (2- (1-hydroxycyclobutyl) benzyl) -4,4- Preparation of bis (hydroxymethyl) -2,3,4,5-tetrahydro-1H-pyrrolo [1,2-a] [1,4] diazalide-1-one)
  • Example 52 Compound 52 (8- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (5-fluoro-2-methoxybenzyl) -4,4-bis ( (Hydroxymethyl) -2,3,4,5-tetrahydro-1H-pyrrolo [1,2-a] [1,4] diazepine-1-one)
  • Example 53 Compound 53 (8- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (2-((difluoromethoxy) methyl) -5-fluorobenzyl ) -4,4-bis (hydroxymethyl) -2,3,4,5-tetrahydro-1H-pyrrolo [1,2-a] [1,4] diazalide-1-one) Preparation
  • Compound 53 was synthesized according to the above synthetic route with reference to the synthetic steps of similar compounds in the compounds of the examples.
  • Example 54 Compound 54 ((R) -7- (5-chloro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2- (5- Fluoro-2- (hydroxymethyl) benzyl) -3-((methoxymethoxy) methyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -Keto) Preparation
  • Compound 54 was synthesized by following the synthetic procedures of similar compounds in the example compounds.
  • Example 55 Compound 55 ((R) -7- (5-chloro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2- (2- Preparation of (1-hydroxycyclopropyl) benzyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one)
  • Example 56 Compound 56 ((R) -7- (5-chloro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2- (5- Fluoro-2-((methoxymethoxy) methyl) benzyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 ( 2H) -Ketone) Preparation
  • Example 57 Compound 57 ((R) -7- (5-chloro-2-((1- (difluoromethyl) -1H-pyrazol-5-yl) amino) pyridin-4-yl) -2 -(5-fluoro-2- (hydroxymethyl) benzyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H)- Ketone)
  • Example 58 Compound 58 ((R) -7- (5-chloro-2-((1-ethyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -2- (5- Preparation of fluoro-2- (hydroxymethyl) benzyl) -3- (methoxymethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one)
  • reaction solution was filtered through diatomaceous earth, the filter cake was washed with ethyl acetate (5 mL * 2), the filtrate was concentrated, and the product was purified by climbing the plate to obtain 29 mg, which is compound 58.
  • Example 149 Compound 148 ((R) -2-((7- (5-chloro-2-((1-methyl-1H-pyrazol-5-yl) amino) pyridin-4-yl) -3 -(Methoxymethyl) -1-oxo 3,4-dihydropyrrolo [1,2-a] pyrazine-2 (1H) -yl) methyl) -4-fluorobenzaldehyde)
  • Step 1 Preparation of Compound 149a
  • reaction mixture was cooled to room temperature and filtered; the filtrate was collected and added to water (2000 mL), and off-white solid particles precipitated; then filtered, the solid particles were collected and dissolved with dichloromethane (400 mL); dried over anhydrous sodium sulfate, filtered, It was concentrated under reduced pressure, and the residue was separated and purified by column chromatography to obtain 27.5 g of a pale yellow foamy solid, that is, 149b.
  • Step 5 Preparation of the compound 149
  • Comparative Example 1 Comparative compound 1 (7- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (2-hydroxy-1- (3- (trifluoromethyl) benzene (Ethyl) ethyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one)
  • Comparative Compound 2 (7- (5-chloro-2- (isopropylamino) pyridin-4-yl) -2- (3-fluorobenzyl) -3,4-dihydropyrrolo [1,2-a] pyrazine-1 (2H) -one, D2).
  • the compound was diluted to the desired concentration with DMSO, 100 ⁇ L was transferred to a 96-well plate, and gradient dilution was performed. Take 10 ⁇ L of compound from each well and mix it with 90 ⁇ L of kinase buffer, and then transfer 5 ⁇ L from each well to a 384-well plate. Add kinase and FAM-labeled peptide and ATP to 1x kinase alkaline buffer to obtain 2.5x enzyme solution and 2.5x peptide solution. Add 10 ⁇ L of 2.5x enzyme solution to the 384-well assay plate. After incubating for 10 minutes at room temperature, add 10 ⁇ L of 2.5x peptide solution to the 384-well plate. After reacting at 28 ° C for a specific time, add 25 ⁇ L of stop buffer to stop the reaction. . After reading the collected data on Caliper, convert the data to percent inhibition:
  • Percent inhibition (maximum value-conversion value) / (maximum value-minimum value) * 100.
  • Example compounds have excellent inhibition on ERK1 and ERK2, most of the compound to inhibit ERK1 and ERK2 IC 50 ⁇ 20nM, embodiments of the compound in Example IC 50 data for inhibition of ERK1 and ERK2 in Table 3.
  • COLO205 cells were plated in a 96-well plate at 2000 cells at 135 ⁇ L / well. After overnight incubation, a compound solution with a gradient concentration was prepared, and 15 ⁇ L of each concentration of the test compound DMSO solution was added to each well cell. 0 nM (all final DMSO concentrations were 0.5%). Incubate at 37 ° C, 5% CO 2 for 120 h. Add 50 ⁇ L of Cell-titer Glo working solution to each well, mix by shaking and incubate at room temperature for 10 minutes, read the luminescence luminescence value with a multi-functional microplate reader, and convert the luminescence value reading to the percent inhibition:
  • Percent inhibition (maximum-reading) / (maximum-minimum) * 100.
  • the “maximum” is the DMSO control; the “minimum” is the acellular control group.
  • Example C Cell proliferation test (HCT 116)
  • HCT 116 cells were plated in a 96-well plate at 1200 cells, 160 ⁇ L / well. After overnight incubation, a compound solution with a gradient concentration was prepared, and 40 ⁇ L of each concentration of the test compound DMSO solution was added to each well cell. The final compound concentration was 30000, 10000, 3333.3, 1111.1, 370.4, 123.5, 41.2, 13.7, 4.6, 0 nM (all final DMSO concentrations were 0.5%). Incubate at 37 ° C, 5% CO 2 for 120 hrs.
  • Percent inhibition (maximum-reading) / (maximum-minimum) * 100.
  • the “maximum” is the DMSO control; the “minimum” is the acellular control group.
  • All the compounds of the examples have good inhibition on HCT 116 cells. Most of the compounds inhibit the IC 50 of HCT 116 cells by less than 100 nM. See Table 5 for the IC 50 data of the compounds of the examples and the inhibition of HCT 116 cells by AZD0364.

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Abstract

La présente invention concerne un composé (tel que représenté dans la formule I) en tant qu'inhibiteur de kinase régulée par un signal extracellulaire (ERK), une composition pharmaceutique associée, son procédé de préparation et son utilisation dans le traitement de maladies médiées par l'ERK. Ledit composé joue un rôle par la régulation d'une pluralité de processus tels que la prolifération cellulaire, l'apoptose, la migration et l'angiogenèse.
PCT/CN2019/090220 2018-06-08 2019-06-06 Inhibiteur d'erk et son utilisation Ceased WO2019233456A1 (fr)

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CN115884966A (zh) * 2020-06-11 2023-03-31 贝达药业股份有限公司 双环化合物及其应用
US12030875B2 (en) 2018-09-07 2024-07-09 PIC Therapeutics, Inc. EIF4E inhibitors and uses thereof
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US12157732B2 (en) 2021-08-25 2024-12-03 PIC Therapeutics, Inc. eIF4E inhibitors and uses thereof
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US12234231B2 (en) 2021-08-25 2025-02-25 PIC Therapeutics, Inc. EIF4E inhibitors and uses thereof
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WO2025255438A1 (fr) 2024-06-07 2025-12-11 Revolution Medicines, Inc. Procédés de traitement d'une maladie ou d'un trouble lié à la protéine ras

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US12157731B2 (en) 2020-03-03 2024-12-03 PIC Therapeutics, Inc. EIF4E inhibitors and uses thereof
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CN114057747A (zh) * 2020-08-07 2022-02-18 贝达药业股份有限公司 双抑制剂及其应用
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US12234231B2 (en) 2021-08-25 2025-02-25 PIC Therapeutics, Inc. EIF4E inhibitors and uses thereof
WO2024229406A1 (fr) 2023-05-04 2024-11-07 Revolution Medicines, Inc. Polythérapie pour une maladie ou un trouble lié à ras
WO2025034702A1 (fr) 2023-08-07 2025-02-13 Revolution Medicines, Inc. Rmc-6291 destiné à être utilisé dans le traitement d'une maladie ou d'un trouble lié à une protéine ras
WO2025080946A2 (fr) 2023-10-12 2025-04-17 Revolution Medicines, Inc. Inhibiteurs de ras
WO2025171296A1 (fr) 2024-02-09 2025-08-14 Revolution Medicines, Inc. Inhibiteurs de ras
WO2025240847A1 (fr) 2024-05-17 2025-11-20 Revolution Medicines, Inc. Inhibiteurs de ras
WO2025255438A1 (fr) 2024-06-07 2025-12-11 Revolution Medicines, Inc. Procédés de traitement d'une maladie ou d'un trouble lié à la protéine ras

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