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WO2019013573A3 - Method for preparing 2-hydroxy-gamma-butyrolactone or 2,4-dihydroxy-butyrate - Google Patents

Method for preparing 2-hydroxy-gamma-butyrolactone or 2,4-dihydroxy-butyrate Download PDF

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Publication number
WO2019013573A3
WO2019013573A3 PCT/KR2018/007923 KR2018007923W WO2019013573A3 WO 2019013573 A3 WO2019013573 A3 WO 2019013573A3 KR 2018007923 W KR2018007923 W KR 2018007923W WO 2019013573 A3 WO2019013573 A3 WO 2019013573A3
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WO
WIPO (PCT)
Prior art keywords
hydroxy
gamma
butyrolactone
butyrate
dihydroxy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2018/007923
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French (fr)
Korean (ko)
Other versions
WO2019013573A2 (en
WO2019013573A9 (en
Inventor
박성훈
김용환
이성국
심증엽
유태현
수만라마
응웬하이남
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UNIST Academy Industry Research Corp
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UNIST Academy Industry Research Corp
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Filing date
Publication date
Application filed by UNIST Academy Industry Research Corp filed Critical UNIST Academy Industry Research Corp
Publication of WO2019013573A2 publication Critical patent/WO2019013573A2/en
Publication of WO2019013573A3 publication Critical patent/WO2019013573A3/en
Publication of WO2019013573A9 publication Critical patent/WO2019013573A9/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01046—Homoserine O-succinyltransferase (2.3.1.46)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01039—Homoserine kinase (2.7.1.39)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
    • C12Y207/02004—Aspartate kinase (2.7.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/08—Phosphoric triester hydrolases (3.1.8)
    • C12Y301/08001—Aryldialkylphosphatase (3.1.8.1), i.e. paraoxonase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/0102—Diaminopimelate decarboxylase (4.1.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01031—Phosphoenolpyruvate carboxylase (4.1.1.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01001—Acetate-CoA ligase (6.2.1.1)

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Furan Compounds (AREA)

Abstract

본 발명은 2-hydroxy gamma butyrol actone (HGBL) 및 이의 전구체인 2， 4-dihydroxybutanoic acid 제조를 위하여 homoserine 생산을 중간단계로 하는 새롭게 제시된 생물합성 경로 및 이 경로를 가지는 유전자 재조합 균주를 제시한다. 2번 위치에 수산화기가 치환된 HGBL의 경우 포토레지스트를 위한 수지，의약품 원료 및 금속 표면의 코팅을 위한 재료로 이용될 수 있는 중요한 중간체로 사용할 수 있다.The present invention proposes a newly proposed biosynthetic pathway and homologous recombinant strains having homoserine production as an intermediate stage for the production of 2-hydroxy gamma butyrol actone (HGBL) and its precursor 2,4-dihydroxybutanoic acid. In the case of HGBL in which hydroxyl group is substituted at position 2, it can be used as an important intermediate which can be used as a resin for photoresist, a raw material for medicines and a material for coating a metal surface.

PCT/KR2018/007923 2017-07-12 2018-07-12 Method for preparing 2-hydroxy-gamma-butyrolactone or 2,4-dihydroxy-butyrate Ceased WO2019013573A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20170088465 2017-07-12
KR10-2017-0088465 2017-07-12

Publications (3)

Publication Number Publication Date
WO2019013573A2 WO2019013573A2 (en) 2019-01-17
WO2019013573A3 true WO2019013573A3 (en) 2019-03-28
WO2019013573A9 WO2019013573A9 (en) 2019-05-02

Family

ID=65002668

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/007923 Ceased WO2019013573A2 (en) 2017-07-12 2018-07-12 Method for preparing 2-hydroxy-gamma-butyrolactone or 2,4-dihydroxy-butyrate

Country Status (2)

Country Link
KR (1) KR102149044B1 (en)
WO (1) WO2019013573A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102547254B1 (en) * 2020-11-06 2023-06-30 주식회사 씨원바이오 Method for Preparing 2-hydroxy-r-butyrolactone Using Enzyme
DE102021101004B3 (en) 2021-01-19 2022-03-10 Technische Universität Dresden, Körperschaft des öffentlichen Rechts Process for the production of 2,4-dihydroxybutyrate or L-threonine using a microbial pathway
CN115948482B (en) * 2023-02-07 2024-02-09 中国科学院天津工业生物技术研究所 Construction method and application of 2, 4-dihydroxybutyric acid biosynthesis pathway

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011100601A1 (en) * 2010-02-11 2011-08-18 Metabolix, Inc. Process for gamma-butyrolactone production
US20140296466A1 (en) * 2013-03-20 2014-10-02 Cj Cheiljedang Corporation Method for Producing Bio-Based Homoserine Lactone and Bio-Based Organic Acid from O-Acyl Homoserine Produced by Microorganisms
US20150147793A1 (en) * 2012-07-11 2015-05-28 Adisseo France S.A.S. Method for the preparation of 2,4-dihydroxybutyrate
WO2016162442A1 (en) * 2015-04-07 2016-10-13 Metabolic Explorer A modified microorganism for the optimized production of 2,4-dihydroxyburyrate with enhanced 2,4-dihydroxybutyrate efflux

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011100601A1 (en) * 2010-02-11 2011-08-18 Metabolix, Inc. Process for gamma-butyrolactone production
US20150147793A1 (en) * 2012-07-11 2015-05-28 Adisseo France S.A.S. Method for the preparation of 2,4-dihydroxybutyrate
US20140296466A1 (en) * 2013-03-20 2014-10-02 Cj Cheiljedang Corporation Method for Producing Bio-Based Homoserine Lactone and Bio-Based Organic Acid from O-Acyl Homoserine Produced by Microorganisms
WO2016162442A1 (en) * 2015-04-07 2016-10-13 Metabolic Explorer A modified microorganism for the optimized production of 2,4-dihydroxyburyrate with enhanced 2,4-dihydroxybutyrate efflux

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE GenPept 19 January 2004 (2004-01-19), Database accession no. AAR95986.1 *
DATABASE Protein 3 June 2015 (2015-06-03), "lactate dehydrogenase [Cupriavidus necator]", XP055585124, retrieved from ncbi Database accession no. WP_011614641.1 *
DATABASE Protein 3 June 2015 (2015-06-03), "lactate dehydrogenase [Lactobacillus delbrueckii]", XP055585127, retrieved from ncbi Database accession no. WP_011543503.1 *
DATABASE Protein 31 May 2015 (2015-05-31), "MULTISPECIES: aminotransferase MtnE [Bacillus]", XP055585120, retrieved from ncbi Database accession no. WP_003244774.1 *

Also Published As

Publication number Publication date
WO2019013573A2 (en) 2019-01-17
WO2019013573A9 (en) 2019-05-02
KR20190007403A (en) 2019-01-22
KR102149044B1 (en) 2020-08-28

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