[go: up one dir, main page]

WO2018208135A3 - 미세구조물을 이용한 세포 및 세포 생성물의 분리, 획득, 분석 및 회수 방법 - Google Patents

미세구조물을 이용한 세포 및 세포 생성물의 분리, 획득, 분석 및 회수 방법 Download PDF

Info

Publication number
WO2018208135A3
WO2018208135A3 PCT/KR2018/005507 KR2018005507W WO2018208135A3 WO 2018208135 A3 WO2018208135 A3 WO 2018208135A3 KR 2018005507 W KR2018005507 W KR 2018005507W WO 2018208135 A3 WO2018208135 A3 WO 2018208135A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyzing
acquiring
separating
cell products
microstructure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2018/005507
Other languages
English (en)
French (fr)
Other versions
WO2018208135A2 (ko
Inventor
권성훈
김준회
장서희
김옥주
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SNU R&DB Foundation
Original Assignee
Seoul National University R&DB Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seoul National University R&DB Foundation filed Critical Seoul National University R&DB Foundation
Priority to US16/612,880 priority Critical patent/US11692191B2/en
Publication of WO2018208135A2 publication Critical patent/WO2018208135A2/ko
Publication of WO2018208135A3 publication Critical patent/WO2018208135A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/12—Apparatus specially adapted for use in combinatorial chemistry or with libraries for screening libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00644—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being present in discrete locations, e.g. gel pads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/0074—Biological products
    • B01J2219/00743—Cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Urology & Nephrology (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 유전자 형태의 라이브러리 스크리닝 기술에 대한 것으로, 항체신약개발을 위한 항체공학 (antibody engineering) 분야에 우선적으로 적용될 수 있으며, 단백질공학 (protein engineering), 대사공학 (metabolic engineering) 등의 다양한 생화학 분자의 유전형/표현형 복합 분석 분야에도 확장성 있게 적용될 수 있는 미세구조물을 이용한 세포 및 세포 생성물의 분리, 획득, 분석 및 회수 방법을 제공한다.
PCT/KR2018/005507 2017-05-12 2018-05-14 미세구조물을 이용한 세포 및 세포 생성물의 분리, 획득, 분석 및 회수 방법 Ceased WO2018208135A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/612,880 US11692191B2 (en) 2017-05-12 2018-05-14 Method for separating, capturing, analyzing and retrieving cells and cell products by using microstructure

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20170059325 2017-05-12
KR10-2017-0059325 2017-05-12

Publications (2)

Publication Number Publication Date
WO2018208135A2 WO2018208135A2 (ko) 2018-11-15
WO2018208135A3 true WO2018208135A3 (ko) 2019-03-28

Family

ID=64102742

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/005507 Ceased WO2018208135A2 (ko) 2017-05-12 2018-05-14 미세구조물을 이용한 세포 및 세포 생성물의 분리, 획득, 분석 및 회수 방법

Country Status (3)

Country Link
US (1) US11692191B2 (ko)
KR (1) KR102127241B1 (ko)
WO (1) WO2018208135A2 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111676132B (zh) * 2020-06-17 2023-07-18 长春长光辰英生物科学仪器有限公司 一种用于激光诱导转移的芯片保护层及细胞分选方法
CN114073996B (zh) * 2021-11-24 2023-04-14 中山大学 一种嵌套式微井阵列芯片及其制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130042857A (ko) * 2011-10-19 2013-04-29 국립대학법인 울산과학기술대학교 산학협력단 하이드로젤을 이용한 세포 패터닝 및 전달 방법 및 이를 이용한 세포 기반 바이오센서
US20130190206A1 (en) * 2010-07-13 2013-07-25 Dublin City University Direct Clone Analysis and Selection Technology
KR20130100149A (ko) * 2010-09-10 2013-09-09 솔리더스 바이오사이언스, 인코포레이티드 마이크로어레이 세포 칩
KR20160019913A (ko) * 2012-12-07 2016-02-22 서울대학교산학협력단 마이크로어레이 기판 상의 생화학 분자들의 분리방법
KR101672654B1 (ko) * 2015-10-16 2016-11-04 서울대학교산학협력단 생체 시료의 선별적 처리방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100634505B1 (ko) 2004-06-16 2006-10-16 삼성전자주식회사 패턴화된 박막층을 갖는 마이크로어레이 기판 및마이크로어레이, 상기 마이크로어레이 기판 및마이크로어레이를 제조하는 방법
KR101575457B1 (ko) 2013-03-07 2015-12-11 서울대학교산학협력단 이종 dna 바코딩 방법
KR101831531B1 (ko) 2015-10-21 2018-02-23 서울대학교산학협력단 생체 시료의 선별적 분석방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130190206A1 (en) * 2010-07-13 2013-07-25 Dublin City University Direct Clone Analysis and Selection Technology
KR20130100149A (ko) * 2010-09-10 2013-09-09 솔리더스 바이오사이언스, 인코포레이티드 마이크로어레이 세포 칩
KR20130042857A (ko) * 2011-10-19 2013-04-29 국립대학법인 울산과학기술대학교 산학협력단 하이드로젤을 이용한 세포 패터닝 및 전달 방법 및 이를 이용한 세포 기반 바이오센서
KR20160019913A (ko) * 2012-12-07 2016-02-22 서울대학교산학협력단 마이크로어레이 기판 상의 생화학 분자들의 분리방법
KR101672654B1 (ko) * 2015-10-16 2016-11-04 서울대학교산학협력단 생체 시료의 선별적 처리방법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GAO, DAN ET AL.: "A microfluidic approach for anticancer drug analysis based on hydrogel encapsulated tumor cells", ANALYTICA CHIMICA ACTA, 14 July 2010 (2010-07-14), pages 7 - 14, XP055584907 *

Also Published As

Publication number Publication date
US20200199583A1 (en) 2020-06-25
WO2018208135A2 (ko) 2018-11-15
US11692191B2 (en) 2023-07-04
KR102127241B1 (ko) 2020-06-26
KR20180124791A (ko) 2018-11-21

Similar Documents

Publication Publication Date Title
WO2020112604A3 (en) Analysis of multiple analytes using a single assay
MX2015016968A (es) Composiciones y metodos para procesamiento de muestras.
PH12020551028A1 (en) Methods, kits and apparatus for expanding a population of cells
EP4289965A3 (en) Method for analyzing rna
CA2867508C (en) Immunofluorescence and fluorescent-based nucleic acid analysis on a single sample
WO2016115537A3 (en) Systems, methods, and apparatus for in vitro single-cell identification and recovery
EP4397767A3 (en) Microcapsule compositions and methods
EP4397774A3 (en) Methods for barcoding nucleic acid molecules from individual cells or cell populations
BR112016025352A2 (pt) métodos de produção de uma biblioteca de clones de células eucarióticas, método de produção de um repertório diverso de ligantes, método de triagem para uma célula de um fenótipo desejado, método de triagem para um ligante que reconhece um alvo, biblioteca, biblioteca in vitro de clones de células eucarióticas, recipiente contendo células eucarióticas, uso de uma nuclease específica de sítio, usos da biblioteca, método para converter as populações de anticorpo de cadeia simples (scfv) em imunoglobulina (ig) ou fragmento, e método que permite a reformatação de quaisquer dois elementos de dna unidos a um vetor
WO2014106004A3 (en) High-throughput system and method for identifying antibodies having specific antigen binding activities
EP4372084A3 (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
GB2568608A (en) Personalized genetic testing
WO2018005811A8 (en) Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
WO2018057959A3 (en) Operation of a library preparation system to perform a protocol on a biological sample
WO2016033251A3 (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
MX2016016902A (es) Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
WO2012148497A3 (en) Identification of polynucleotides associated with a sample
EP4273262A3 (en) Methods and devices for analyzing particles
EP4345460A3 (en) Assay apparatuses, methods and reagents
EA201391245A1 (ru) Интеррогативные клеточные анализы и их применение
GB2525804A (en) Methods and compositions for classification of samples
WO2017189357A3 (en) Charged surface reversed phase chromatographic materials method for analysis of glycans modified with amphipathic, strongly basic moieties
WO2013010134A3 (en) Systems, apparatus and methods for biochemical analysis
SG10201811714UA (en) Substance sealing method and target molecule detecting method
EP4512527A3 (en) Microfluidic siphoning array for nucleic acid quantification

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18797938

Country of ref document: EP

Kind code of ref document: A2