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WO2018005811A8 - Differential tagging of rna for preparation of a cell-free dna/rna sequencing library - Google Patents

Differential tagging of rna for preparation of a cell-free dna/rna sequencing library Download PDF

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Publication number
WO2018005811A8
WO2018005811A8 PCT/US2017/040027 US2017040027W WO2018005811A8 WO 2018005811 A8 WO2018005811 A8 WO 2018005811A8 US 2017040027 W US2017040027 W US 2017040027W WO 2018005811 A8 WO2018005811 A8 WO 2018005811A8
Authority
WO
WIPO (PCT)
Prior art keywords
rna
cell
preparation
free dna
sequencing library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2017/040027
Other languages
French (fr)
Other versions
WO2018005811A1 (en
Inventor
Matthew Larson
H. John KIM
Nick EATTOCK
Arash Jamshidi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Grail Inc
Original Assignee
Grail Inc
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Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=59351088&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2018005811(A8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to EP21151626.5A priority Critical patent/EP3831958B1/en
Priority to CN201780049600.9A priority patent/CN109563541A/en
Priority to CA3024984A priority patent/CA3024984C/en
Priority to AU2017290237A priority patent/AU2017290237B2/en
Priority to EP23189098.9A priority patent/EP4257701A3/en
Application filed by Grail Inc filed Critical Grail Inc
Priority to EP17740184.1A priority patent/EP3478856B1/en
Publication of WO2018005811A1 publication Critical patent/WO2018005811A1/en
Publication of WO2018005811A8 publication Critical patent/WO2018005811A8/en
Anticipated expiration legal-status Critical
Priority to AU2021200391A priority patent/AU2021200391B2/en
Priority to AU2023251444A priority patent/AU2023251444A1/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

In various aspects, the present disclosure provides methods, compositions, reactions mixtures, kits, and systems for sequencing both RNA and DNA from a single source sample. In some embodiments, RNA is treated so as to differentiate RNA sequences from DNA sequences derived from the same sample. In some embodiments, the RNA and DNA are cell-free polynucleotides.
PCT/US2017/040027 2016-06-30 2017-06-29 Differential tagging of rna for preparation of a cell-free dna/rna sequencing library Ceased WO2018005811A1 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP17740184.1A EP3478856B1 (en) 2016-06-30 2017-06-29 Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
CN201780049600.9A CN109563541A (en) 2016-06-30 2017-06-29 It is used to prepare Cell-free DNA/RNA sequencing library RNA otherness labeling method
CA3024984A CA3024984C (en) 2016-06-30 2017-06-29 Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
AU2017290237A AU2017290237B2 (en) 2016-06-30 2017-06-29 Differential tagging of RNA for preparation of a cell-free DNA/RNA sequencing library
EP23189098.9A EP4257701A3 (en) 2016-06-30 2017-06-29 Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
EP21151626.5A EP3831958B1 (en) 2016-06-30 2017-06-29 Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
AU2021200391A AU2021200391B2 (en) 2016-06-30 2021-01-21 Differential tagging of RNA for preparation of a cell-free DNA/RNA sequencing library
AU2023251444A AU2023251444A1 (en) 2016-06-30 2023-10-17 Differential Tagging Of RNA For Preparation Of A Cell-Free DNA/RNA Sequencing Library

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662357281P 2016-06-30 2016-06-30
US62/357,281 2016-06-30
US201662368025P 2016-07-28 2016-07-28
US62/368,025 2016-07-28

Publications (2)

Publication Number Publication Date
WO2018005811A1 WO2018005811A1 (en) 2018-01-04
WO2018005811A8 true WO2018005811A8 (en) 2018-04-05

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PCT/US2017/040027 Ceased WO2018005811A1 (en) 2016-06-30 2017-06-29 Differential tagging of rna for preparation of a cell-free dna/rna sequencing library

Country Status (6)

Country Link
US (3) US10144962B2 (en)
EP (3) EP3478856B1 (en)
CN (1) CN109563541A (en)
AU (3) AU2017290237B2 (en)
CA (1) CA3024984C (en)
WO (1) WO2018005811A1 (en)

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Also Published As

Publication number Publication date
CA3024984C (en) 2021-12-07
AU2021200391A1 (en) 2021-03-18
CN109563541A (en) 2019-04-02
EP4257701A2 (en) 2023-10-11
US20190062831A1 (en) 2019-02-28
AU2021200391B2 (en) 2023-08-03
US20180002749A1 (en) 2018-01-04
AU2017290237B2 (en) 2020-10-22
AU2023251444A1 (en) 2023-11-09
EP3831958B1 (en) 2023-09-06
US20220010372A1 (en) 2022-01-13
AU2017290237A1 (en) 2018-12-06
EP4257701A3 (en) 2023-12-20
EP3478856B1 (en) 2021-01-27
EP3831958A1 (en) 2021-06-09
US10144962B2 (en) 2018-12-04
WO2018005811A1 (en) 2018-01-04
CA3024984A1 (en) 2018-01-04
US11180801B2 (en) 2021-11-23
EP3478856A1 (en) 2019-05-08

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