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WO2018015543A1 - Pointe de pipette destinée à un dispositif de pipetage automatique - Google Patents

Pointe de pipette destinée à un dispositif de pipetage automatique Download PDF

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Publication number
WO2018015543A1
WO2018015543A1 PCT/EP2017/068494 EP2017068494W WO2018015543A1 WO 2018015543 A1 WO2018015543 A1 WO 2018015543A1 EP 2017068494 W EP2017068494 W EP 2017068494W WO 2018015543 A1 WO2018015543 A1 WO 2018015543A1
Authority
WO
WIPO (PCT)
Prior art keywords
pipette
pipette tip
electrode
tip
sample liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2017/068494
Other languages
German (de)
English (en)
Inventor
Philipp Ott
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tecan Trading AG
Original Assignee
Tecan Trading AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CH00950/16A external-priority patent/CH712735A1/de
Priority claimed from CH00523/17A external-priority patent/CH712764A2/de
Application filed by Tecan Trading AG filed Critical Tecan Trading AG
Priority to US16/308,783 priority Critical patent/US11291988B2/en
Priority to EP17740406.8A priority patent/EP3452222B1/fr
Publication of WO2018015543A1 publication Critical patent/WO2018015543A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1016—Control of the volume dispensed or introduced
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
    • G01D5/00—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable
    • G01D5/12—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means
    • G01D5/14—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage
    • G01D5/24—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage by varying capacitance
    • G01D5/241—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage by varying capacitance by relative movement of capacitor electrodes
    • G01D5/2417—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage by varying capacitance by relative movement of capacitor electrodes by varying separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
    • G01F23/00—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm
    • G01F23/22—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water
    • G01F23/26—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields
    • G01F23/263—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields by measuring variations in capacitance of capacitors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/021—Identification, e.g. bar codes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/54—Labware with identification means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
    • G01F23/00—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm
    • G01F23/22—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water
    • G01F23/26—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields
    • G01F23/263—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields by measuring variations in capacitance of capacitors
    • G01F23/268—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields by measuring variations in capacitance of capacitors mounting arrangements of probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N2035/1025—Fluid level sensing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/103—General features of the devices using disposable tips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1011—Control of the position or alignment of the transfer device

Definitions

  • Patent application is included, the priority of
  • the present invention relates to the technical field of automated fluid processing systems, and more particularly relates to pipette tips, especially disposable tips (namely, disposable tips, abbreviated "DiTis”) for pipetting, ie, aspirating and / or dispensing, of liquids. Furthermore, the present invention relates to pipetting devices with such Pipette tips, method for determining the volume of a sample liquid in such pipette tips, method for detecting such pipette tips on a
  • Pipetting device uses of such pipette tips and a set of such pipette tips.
  • Fluid handling systems designed to handle fluid volumes.
  • Liquid processing systems include in particular
  • pipette tips are designed for single use and are commonly referred to as “disposable pipette tips” or English as “disposable tips” (abbreviated to “DiTis”).
  • dispenser pipette tips or English as “disposable tips” (abbreviated to "DiTis”).
  • Pipetting device this is able to detect, if any, a pipette tip on the pipette tube
  • a pipette tip according to the invention is provided for connection to a pipette tube by a pipetting device and shaped as an elongate tube which forms a pipette body having at its one, first end an opening for aspirating and / or dispensing Liquids, and at its other end for (fluid-tight) connection to the pipette tube
  • an electrode as a volume measuring electrode of a measuring capacitor and a second electrode as immersion detection electrode, wherein the first electrode is located on an outer surface of the pipette body or in
  • Pipette body is embedded and the second
  • Electrode is at least partially located on an inner surface of the pipette body.
  • the second electrode at least partially on an inner surface of the pipette body
  • the second electrode is at least partially exposed to the interior of the pipette body.
  • the second electrode may be partially embedded in the material of the pipette body in such a way that it is still partly or partially exposed to the interior of the pipette body. It does not matter whether the second electrode is embedded in the pipette body or even at least partially along the outer surface of the
  • Pipette body runs as long as one or more sections or contact points of the second electrode are still exposed to the interior of the pipette body and thus at least partially contact the liquid in the interior of the pipette body.
  • the first electrode and second electrode are electrically conductive and at least partially contact the second electrode the liquid on the inner surface of the
  • Pipette body made of an electrically non-conductive
  • non-conductive plastic such as non-conductive polypropylene
  • the second electrode is arranged substantially outside the relevant measuring capacitor.
  • the second electrode is outside the measuring capacitor.
  • Electrode and second electrode may be arranged substantially opposite to each other.
  • the first electrode contains at least two first electrodes
  • Partial electrodes and the second electrode includes at least two second partial electrodes, wherein the first partial electrodes are arranged opposite to each other and the second partial electrodes are arranged opposite to each other.
  • the first electrode is elongated, in particular
  • Angular range between 20 ° and 270 °, preferably in an angular range of 40 °.
  • the first electrode is elongated, in particular
  • the first electrode in particular a
  • staircase-shaped width profile in the axial direction and the width of the first electrode, e.g. at the first end is the lowest, and the width in the axial direction is gradually increased.
  • the first electrode does not extend to the opening at the first end and is in particular spaced from this opening in a range of 3 mm to 6 mm, preferably in a range of 4 mm to 6 mm, particularly preferably 5 mm.
  • the second electrode is strip-shaped and extends axially along the tube, and wherein they
  • the second electrode contacts the liquid in the
  • Pipette body wherein the contacting points in an angular range to each other between 90 ° and 270 °, preferably 180 °, are arranged.
  • the contacting points in an angular range to each other between 90 ° and 270 °, preferably 180 °, are arranged.
  • the contacting points are arranged in particular at 90 ° and 270 ° when the first electrode is at 0 °.
  • the second electrode extends to the opening at the first end or the opening at the first end is at least partially formed by the second electrode.
  • Pipette tip a disposable pipette tip, which
  • a pipetting device comprises at least one
  • Capacitance measuring unit wherein the pipette tube is formed at its one, first end for fluid-tight connection of a proposed pipette tip, and is connected at its other end to the pressure generating means.
  • capacity imaging part of the complex
  • Impedance can also change the resistive component (real part of the complex impedance).
  • resistive component real part of the complex impedance
  • a first electrical contact for producing a first electrical contact in a region for connecting the pipette tip, a first electrical contact for producing a first electrical contact
  • volume measuring electrode present which is located on an outer surface of the pipette body or embedded in the pipette body.
  • a second electrical contact may be provided for establishing an electrical connection to a second electrode as a dip detection electrode located at least partially on an inner surface of the pipette body.
  • Pipetting device is the liquid in one Sample container, which is electrically conductively or capacitively coupled to a conductive work table surface which is connected to ground.
  • Pipetting device is the first electrical contact via an electrical conductor connected to the measuring unit and is the second electrical contact via the liquid and a capacitive coupling of the liquid over the
  • the measuring unit is designed to function as a function of a measured capacity of a
  • Measuring capacitor which through the first electrode and at least part of one in the pipette tip
  • absorbable sample liquid is formed as a counter electrode, a volume in the pipette tip
  • Pipetting device further comprises a detection unit for detecting whether the pipette tip at the first end of
  • Pipette tube is connected, and / or for detecting a characteristic feature of the pipette tip based on a measurement of a further capacity, wherein the further capacity is dependent on one or more of the following properties:
  • Pipette tip exists, more particularly one
  • Electrode consists; a geometry (such as a length, width, thickness
  • Measuring a capacitance of a measuring capacitor comprising a first electrode, which is located on an outer surface of the pipette body or in
  • this includes
  • a second electrode as immersion detection electrode (or as immersion contact), wherein the second electrode is at least partially on an inner surface of the pipette body.
  • Pipetting device with a pipette tube, which at its one, first end for releasably receiving a
  • Is formed pipette tip for aspirating or dispensing a liquid and which is operatively connected at its other end to a pressure generating means, the method comprising measuring a capacity which is dependent on one or more of the following
  • Pipette tip exists, more particularly one
  • Electrode consists;
  • a geometry such as a length, width, thickness
  • a method of manufacturing comprises forming the pipette tip as an elongate tube constituting a pipette body from a first material, forming a first electrode from a second material at the pipette tip an outer surface of the
  • the second material and the third material may be the same.
  • the steps of molding by means of a Mehrkomponentensprit zgussvons in particular the same Mehrkomponentensprit zgussvons, sequentially or simultaneously with the molding of the pipette tip and the first and / or second electrode.
  • a first step the non-conductive first material for molding the
  • Pipette tip to be injected. After this is solidified (e.g., about 6 seconds), the conductive second and third materials can be injected. Of course, further manufacturing processes can be realized.
  • a further aspect of the present invention relates to a use of the proposed pipette tip for determining a volume of a sample liquid, which is located in the pipette tip, with the aid of the first electrode as Volumenmesselektrode at the pipette tip, wherein the first electrode on an outer
  • Pipette body is embedded.
  • Another aspect of the present invention relates to a use of the proposed pipette tip for detecting immersion of the pipette tip into the sample liquid by means of the second electrode
  • a set of proposed pipette tips comprises at least two types of pipette tips, wherein the at least two types differ in that
  • Fig. La is a schematic representation of a first
  • FIG. 2a Volume measuring electrode in a front view
  • Fig. 3a is a schematic representation of a fifth
  • Fig. 4a is a schematic representation of a dip
  • Fig. 5a is a schematic representation of an aspiration and dispensing process a
  • Fig. 6a is a schematic representation of a
  • FIG. 7 is a schematic representation of a
  • Fig. 8 is a schematic representation of three
  • Fig. 9 is a representation of a pipette tip according
  • Fig. La is a schematic representation of a first embodiment of a pipette tip 1 with a strip-shaped volume measuring electrode 7 and a
  • strip-shaped immersion detection electrode 10 is shown in a sectional view from the side, while in Fig. Lb) the pipette tip 1 is shown in a view from the front (on the volume measurement electrode).
  • the elongate tube, which forms a pipette body 4 has a substantially conical shape.
  • the cross section of the tube may take any shape, for example, oval, rectangular, etc.
  • the volume measuring electrode 7 is located on an outer surface of the pipette body 4 and the
  • Immersion detection electrode 10 on an inner surface of the pipette body 4.
  • the immersion detection electrode 10 is shown in phantom when viewed through the material of the pipette body 4.
  • the immersion detection electrode 10 is located within the wall of the pipette body 4 and faces the interior of the pipette body 4.
  • Sample liquid the immersion detection electrode but also partially, e.g. periodically
  • the immersion detection electrode 10 extends to the
  • volume measuring electrode 7 e.g. not to the opening 5
  • Volumenmesselektrode 7 and immersion detection electrode 10 are electrically conductive and can be arranged substantially opposite each other (this is in the Discussed in more detail below).
  • Immersion detection electrode 10 may be e.g. consist of the same material as the volume measuring electrode 7.
  • the pipette body 4 has an opening 5 for aspirating and / or dispensing liquids.
  • the other end is designed for fluid-tight connection to a pipette tube 2, the pipette tube 2 for example having a cone as an adapter for this purpose.
  • the Volumenmesselektrode 7 acts as a first electrode of a measuring capacitor, which further at least a part of a recordable in the pipette tip 1
  • Sample liquid comprises as a counter electrode.
  • the pipette body 4 forms the dielectric of this
  • the pipette body 4 may consist of an electrically non-conductive material, which forms the dielectric, in particular a non-conductive plastic, such as non-conductive polypropylene.
  • the volume measuring electrode 7 is in the example shown as a narrow strip on the outer surface of the
  • Pipette body 4 is arranged and extends axially along the pipette tip 1 (with central axis a).
  • Volume measuring electrode 7 has e.g. a width in a range of 0.8 mm to 5 mm, preferably in the range of 0.8 mm to 2 mm, particularly preferably 1 mm. At its upper end, the volume measuring electrode 7 has a
  • the immersion detection electrode 10 is also formed here as a narrow strip and extends axially along the pipette tip 1 (with central axis a). With the help of this immersion detection electrode 10 can be determined when the pipette tip 1, the surface of the
  • Immersion detection electrode 10 at least partially on the inner surface of the pipette body 4 and is the interior of the pipette body 4 at least partially
  • Immersion detection electrode 10 is partially embedded in the wall of the pipette body 4.
  • Sample liquid is made possible, especially if the sample liquid has a low conductivity. Details will be given below in connection with u.a. Fig. 6 explained in more detail.
  • Fig. Lc is a schematic representation of a second embodiment of a pipette tip 1 with a staircase-shaped Volumenmesselektrode 7 shown in a view from the front.
  • Volume measuring electrode 7 When using such Volume measuring electrode 7 occur each capacity jumps (in the volume curve, ie the volume of
  • volume measuring electrode 7 exceeds.
  • the level or the volume of the sample liquid in the pipette tip 1 largely independent of the
  • Immersion detection electrode disposed at least partially on the inner surface of the pipette body 4.
  • Figures 2a) -c) show the first and second
  • the opening 5 is in the region of the center axis of the
  • Pipette tip 1 is arranged. As can be seen, in FIGS. 2a) -c), the volume measuring electrode 7 is arranged on the outer surface of the pipette body 4, while the immersion detection electrode 10 at least partially forms or contacts the inner surface of the pipette body 4.
  • Immersion detection electrode 10 may be axially along the
  • Pipette body 4 run and in this case be strip-shaped, as shown in Fig. 2a).
  • Immersion detection electrode 10 may alternatively also be arcuate and circulate the pipette body 4 to a certain inner circumference arcuate, as shown in Fig. 2b).
  • the arcuate immersion detection electrode 10 may circulate the pipette body 4 by half of its inner circumference (180 °).
  • the arcuate immersion detection electrode may orbit the pipette body 4 inside by three quarters of its inner circumference (270 °) (not shown).
  • Another advantageous circulation angle is 220 °. As far as technically reasonable, are more
  • first partial electrodes 7T1,7T2 contain at least two first partial electrodes 7T1,7T2 and the second electrode at least two second partial electrodes 10T1,10T2 contain, wherein the first partial electrodes
  • Fig. 2d shows a fourth embodiment of the pipette tip 1 in a cross-sectional view from above with a coating 8 over the volume measuring electrode 7 and over the remaining pipette body 4 (i.e.
  • coating 8 can only be used in one area Volume measuring electrode 7 may be present, for example, to prevent the volume measuring electrode 7 with the
  • Pipette tip 1 is lowered too far into the sample liquid.
  • the entire pipette tip 1 may be coated with a coating 8, e.g. with a hydrophobic, oleophobic or antibacterial coating 8.
  • Fig. 3a is a schematic representation of a fourth embodiment of a pipette tip 1 with an embedded in the pipette body 4
  • volume measuring electrode 7 and a at least partially arranged on the inner surface of the pipette body 4 immersion detection electrode 10 in a sectional view from the side.
  • the volume measuring electrode 7 is from
  • Dielectric serves. The volume measuring electrode 7 and
  • Immersion detection electrode 10 are strip-shaped
  • the immersion detection electrode 10 extends to the opening 5, while the Volumenmesselektrode 7 can extend almost to the opening 5.
  • the same pipette tip 1 is shown in Fig. 3b) in a schematic view from above. In this case, the volume measuring electrode 7 is enveloped on all sides by the material of the pipette body 4 and enclosed therein. Again, this is the
  • Immersion detection electrode 10 in sections inside the pipette body 4 facing and exposed and can contact sample liquid contained therein.
  • Fig. 4a illustrates in a schematic representation a use of the proposed pipette tip 1 for immersion detection (-> cLLD), i. for the determination of
  • Sample liquid 6 which is located in a container 19, such. a tub, a sample tube or a "well” in a microplate, immersed (and
  • the immersion detection electrode 10 is
  • Immersion detection electrode 10 touches the sample liquid 6 and immersed in this, and then drops abruptly once the immersion detection electrode 10 is pulled out of the sample liquid 6.
  • Fig. 5a illustrates the use of the proposed pipette tip 1 for measuring the volume of the
  • Geometry of Volumenmesselektrode 7 due to the ever higher sample liquid column in the pipette tip 1, which forms an increasing counter electrode to Volumenmesselektrode 7 then drops linearly during dispensing and finally makes a jump down as soon as the immersion detection electrode 10 is pulled out of the sample liquid 6 ,
  • Fig. 6a illustrates schematically with a sample liquid 6 'filled pipette tip 1' according to the prior art
  • Fig. 6b schematically with a
  • Sample liquid 6 filled pipette tip 1 according to an embodiment of the invention represents.
  • Sample liquid 6,6 is assumed here a liquid with a low conductivity. It is further assumed in both cases that the
  • Immersion detection electrode 10 'on the outer surface a pipette body 4 'of the pipette tip 1' is mounted, as shown in Fig. 6a).
  • the immersion detection electrode 10 is at least partially on the inner surface of the
  • the dip detection electrode 10 may contact the liquid column in the pipette tip 1 at regular intervals (not shown), as explained in more detail below.
  • the immersion detection electrode 10 directly on the inner surface of the pipette body 4, the immersion detection electrode 10 is always on the same
  • FIG. 7 illustrates in a simplified schematic representation an embodiment of a
  • Pipetting device according to the invention. This illustration shows the pipette tube 2 with an attached
  • Pipette tip 1 which was previously removed from a receptacle 16 of a pipette tip holder 15 and now moved by a robot arm 14 on a liquid processing system 3 vertically upward and thus of the
  • Pipette tip holder 15 is removed.
  • a capacitance measuring unit 11 which with the pipette tube 2 and thus also with the pipette tip 1 as the first electrode and the work table or the work surface 17 and thus also with the pipette tip zenhalterung 15 (or a component thereof) as the second (counter) Connected electrode is, determine the (absolute) capacity of the measuring capacitor comprising these two electrodes.
  • the measured value of the capacitance will be different. For this purpose, the measured value of the capacity in one
  • Detection unit 12 compared to various reference values, each reference value is characteristic of a particular type or type of
  • the detection unit 12 then gives information about whether a pipette tip 1 at the
  • Pipette tube 2 is connected, and if so what type or type of pipette tip 1 it is, to a control unit 13 of a drive for the robot arm 14 on.
  • the robot arm 14 in this case has a first
  • horizontal movement axis (x direction, e.g., front and back), a second horizontal movement axis (y direction, e.g., left and right), and a vertical movement axis (z direction, e.g., down and up).
  • the Pipettenspit zenhalterung 15 can, for example, also
  • the pipette tip holder 15 provides a separate / dedicated one "Measuring station" for pipette tip detection within the liquid processing system 3.
  • FIGS. 8a) -c) show, by way of example, three pipette tips 1 with a respective differently wide volume measuring electrode 7.
  • the three pipette tips 1 can be a set of three
  • volume measuring electrodes 7 result, can be distinguished from each other and recognized. Additionally or alternatively, although not shown in the figures, the respective immersion detection electrodes of the three pipette tips 1 may each have different widths, whereby the three pipette tips 1 are proposed by means of the proposed one
  • Detection method based on the different capacities, which result from the different widths of the immersion detection electrodes, can be distinguished from each other and recognized.
  • Figures 9a) -c) illustrate the pipette tip 1 according to the invention in a further embodiment.
  • the first electrode 7 is as
  • Pipette body 4 is arranged.
  • the second electrode 10 as a dip detection electrode is disposed so as to be partially on the inner surface of the
  • Pipette body 4 is located. Stand out for this
  • Immersion detection electrode 10 orbits the pipette body 4 at a circulation angle of e.g. > 180 °, for example 220 °.
  • a circulation angle e.g. > 180 °, for example 220 °.
  • Contact points 20 may be arranged offset to one another.
  • the respective opposite contacting points 20 can at a fixed offset to each other
  • the front tip of the pipette body 4 in the region of the opening 5 thereof is completely circumferential with the material of the second electrode 10 in the example shown
  • the first electrode 7 is disposed in the opening part (sector) of the partially revolving second electrode 10.
  • the second electrode 10 is centrally located therein. As shown in Fig. 9c), this is
  • Electrode 7 is enabled. This allows the first
  • Electrode 7 are electrically connected to the pipette tube (see Fig. 7).
  • Control unit for controlling the movement of the drive of the pipetting robot
  • Pipetting robot e.g., forward and backward
  • Pipetting robot (for example, to the left and right) z vertical axis of movement of the pipetting robot

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  • Physics & Mathematics (AREA)
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  • Engineering & Computer Science (AREA)
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  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
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  • Pathology (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Analytical Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Electromagnetism (AREA)
  • Thermal Sciences (AREA)
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  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

La présente invention concerne des pointes de pipette (1) destinées à être reliées à un tube de pipette (2) d'un dispositif de pipetage qui sert à prélever et à émettre des liquides. La pointe de pipette (1) selon l'invention prend la forme d'un tube allongé qui forme un corps de pipette (4) qui présente à l'une de ses extrémités une ouverture (5) et est conçu à son autre extrémité pour être raccordé au tube de pipette (2), la pointe de pipette (1) présentant une première électrode (7) en tant qu'électrode de mesure de volume d'un condensateur de mesure et une deuxième électrode (10) en tant qu'électrode de détection d'immersion, la première électrode (7) se trouvant sur une surface extérieure du corps de pipette (4) ou étant intégrée au corps de pipette (4) et la deuxième électrode (10) se trouvant au moins en partie sur la surface intérieure du corps de pipette (4). L'invention concerne par ailleurs des dispositifs de pipetage dotés d'une pointe de pipette (1) de ce type, des procédés pour déterminer le volume d'un échantillon de liquide dans une pointe de pipette (1) de ce type, des procédés pour reconnaître une pointe de pipette (1) sur un dispositif de pipetage, des procédés pour réaliser une pointe de pipette (1) de ce type, des utilisations d'une pointe de pipette (1) de ce type et un ensemble de pointes de pipette (1) de ce type.
PCT/EP2017/068494 2016-07-22 2017-07-21 Pointe de pipette destinée à un dispositif de pipetage automatique Ceased WO2018015543A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US16/308,783 US11291988B2 (en) 2016-07-22 2017-07-21 Pipette tip for an automated pipetting device
EP17740406.8A EP3452222B1 (fr) 2016-07-22 2017-07-21 Pointe de pipette destinée à un dispositif de pipetage automatique

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
CH00950/16 2016-07-22
CH00950/16A CH712735A1 (de) 2016-07-22 2016-07-22 Pipettiervorrichtung mit einem Flüssigkeitsvolumensensor und Flüssigkeitsbearbeitungssystem.
CH00159/17A CH712761A2 (de) 2016-07-22 2017-02-10 Pipettiervorrichtung mit einer Pipettenspitzenerkennungseinheit und Verfahren zum Erkennen einer Pipettenspitze an einer Pipettiervorrichtung.
CH00159/17 2017-02-10
CH00523/17 2017-04-19
CH00523/17A CH712764A2 (de) 2016-07-22 2017-04-19 Pipettenspitze aufweisend eine Volumenmesselektrode sowie Verfahren zu deren Herstellung und Pipettiervorrichtung.

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WO2018015543A1 true WO2018015543A1 (fr) 2018-01-25

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PCT/EP2017/068200 Ceased WO2018015419A1 (fr) 2016-07-22 2017-07-19 Pointe de pipette pour dispositif de pipetage automatique et procédé pour la réaliser
PCT/EP2017/068495 Ceased WO2018015544A1 (fr) 2016-07-22 2017-07-21 Dispositif de pipettage, système de traitement de liquide et procédé de fonctionnement d'un système de traitement de liquide
PCT/EP2017/068494 Ceased WO2018015543A1 (fr) 2016-07-22 2017-07-21 Pointe de pipette destinée à un dispositif de pipetage automatique
PCT/EP2017/068497 Ceased WO2018015545A1 (fr) 2016-07-22 2017-07-21 Procédé pour déterminer la position d'un bras robotisé dans un système de manipulation de liquide et système de manipulation de liquide correspondant

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PCT/EP2017/068495 Ceased WO2018015544A1 (fr) 2016-07-22 2017-07-21 Dispositif de pipettage, système de traitement de liquide et procédé de fonctionnement d'un système de traitement de liquide

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