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WO2018000110A1 - Kit de réactif présentant une fonction d'isolation à froid, une fonction d'alarme et une fonction d'alerte d'addition d'échantillon - Google Patents

Kit de réactif présentant une fonction d'isolation à froid, une fonction d'alarme et une fonction d'alerte d'addition d'échantillon Download PDF

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Publication number
WO2018000110A1
WO2018000110A1 PCT/CN2016/085917 CN2016085917W WO2018000110A1 WO 2018000110 A1 WO2018000110 A1 WO 2018000110A1 CN 2016085917 W CN2016085917 W CN 2016085917W WO 2018000110 A1 WO2018000110 A1 WO 2018000110A1
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WO
WIPO (PCT)
Prior art keywords
reagent
function
kit
experiment
sounding device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2016/085917
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English (en)
Chinese (zh)
Inventor
彭鹏
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Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to PCT/CN2016/085917 priority Critical patent/WO2018000110A1/fr
Publication of WO2018000110A1 publication Critical patent/WO2018000110A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters

Definitions

  • the invention and the field of biotechnology relate to a kit having a cold keeping function, an alarm function and a sample reminding function.
  • kits standardizes the steps of common biological experiments, providing the experimenter with the reagents to be prepared and the optimized loading method, which greatly increases the speed of the experiment and the standardization of the results.
  • the number of reagents in the existing kits is also increasing, and the loading step is becoming more and more complicated.
  • the general kit contains Taq enzyme, buffer, chromogenic dye and sterile ultrapure water.
  • the reagents required by the experimenter include at least the upstream primer. Downstream primers and template, a total of 7 reagents together to prepare a fluorescent quantitative PCR reaction system.
  • the experimenter will have the phenomenon of missing, adding, adding, etc. during the process of adding samples. The reason is that there are many kinds of reagents, and the process of loading cannot be well identified and marked. When other urgent matters cause the ongoing experiment to be interrupted, the error is in place. Inevitably.
  • the present invention provides a kit having a cold keeping function, an alarm function, and a sample reminding function.
  • kits with a cold keeping function, an alarm function and a sample reminding function comprising a kit body and a lid, wherein the kit body is provided with a sounding device, a control unit and a small battery The sounding device, the control unit and the battery are electrically connected; the outer wall of the reagent hole is provided with a refrigeration device and a temperature control probe, and the refrigeration device, the temperature control probe and the control unit are electrically connected.
  • the inner layer of the kit body is further provided with a lining plate, and the lining plate is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicator light is further arranged between the two.
  • the lining plate is spaced apart from the opening of the casing such that the lining plate forms an open receiving space with the casing; the reagent rotating disk is provided with a plurality of reagent holes, and A power mechanism is connected.
  • the kit body is further provided with an inductor, the sensor is disposed corresponding to the bottom of the pipette tip collecting hole, and the sensor is controlled by sensing the bottom of the pipette tip collecting hole
  • the power mechanism drives the rotation of the reagent rotating disk and the color of the arrow indicator light.
  • the kit body has a two-layer structure, and the control unit, the sounding device and the small battery are disposed between the inner layer and the outer layer of the kit body.
  • the number of the reagent wells is six.
  • the sounding device is a buzzer, and the number is two.
  • control unit is a single chip microcomputer.
  • the small battery is a lithium ion battery.
  • the inductor is connected to the bottom of the pipette tip collecting hole by a spring.
  • the reagent rotating disk is provided with 8 reagent holes; the power mechanism connected thereto is a stepping motor, and is connected to the inductor and controlled by the inductor.
  • the arrow indicator is made of an LED, connected to the sensor, and controlled by the sensor.
  • the invention can provide the required pre-cooling environment (2-8 ° C) for the reagent by setting the sounding device, the control unit and the battery in the body of the reagent box and the refrigeration device and the temperature control probe on the outer wall of the reagent hole, and can pass the temperature control When the probe detects the temperature, once the setting range is exceeded, the sounding device immediately alarms the experimenter to reduce the probability of the experiment failure. At the same time, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding errors. The resulting experiment failed.
  • Fig. 1 is an overall configuration diagram of the present invention.
  • Fig. 2 is a structural diagram of the reagent well.
  • Figure 3 is a schematic view showing the structure of the reagent rotating disk, the arrow indicator lamp, and the pipette tip head collecting hole shown in Figure 1.
  • a kit with a cold keeping function, an alarm function and a sample reminding function includes a kit body 10 and a lid 20 , and the kit body is provided with a lining plate 30 , a sounding device 40 and a control Unit (not shown) and small battery (not shown). Electrical connection between the sounding device, the control unit and the battery;
  • the outer wall of the reagent hole is provided with a refrigerating device 381 and a temperature control probe 382, and the refrigerating device, the temperature control probe and the control unit are electrically connected.
  • the lining plate 30 is provided with a pipette tip collecting hole 32 and a reagent rotating disk 36.
  • An arrow indicator light 34 is further disposed between the two.
  • the reagent rotating disk 36 is provided with a plurality of reagent holes. 38.
  • the inductor 60 is disposed in the casing 10, the inductor 60 is disposed corresponding to the bottom of the pipette tip collecting hole 32, and the inductor 60 controls the stepping motor 70 to drive the reagent through the bottom of the inductive pipette tip collecting hole 32.
  • the rotation of the rotating disk 36 and the color of the arrow indicator light 34 are illuminated.
  • the inductor 60 is connected to the bottom of the pipette tip collecting hole 32 by the spring 50.
  • the discarded tip is driven into the inductive pipette tip head collecting hole 32, and the inductor 60 controls the stepping motor 7 through the bottom of the inductive pipette tip collecting hole 32.
  • 0 drives the color of the reagent rotating disk 36 to rotate and the arrow indicator light 34 to light up.
  • the color of the arrow indicator light 34 is in one-to-one correspondence with the reagent holes on the reagent rotating disk 36.
  • the eight colors of red, orange, yellow, green, cyan, blue, purple, and white correspond to 1-8 on the reagent rotating disk 36, respectively. Hole.
  • control stepper motor 70 drives the reagent rotary disk 36 to rotate 45° to its next reagent hole, and controls the arrow indicator. 34 lights up the color of the corresponding reagent hole.
  • the invention can provide the required pre-cooling environment (2-8 ° C) for the reagent by setting the sounding device, the control unit and the battery in the body of the reagent box and the refrigeration device and the temperature control probe on the outer wall of the reagent hole, and can pass the temperature control When the probe detects the temperature, once the setting range is exceeded, the sounding device immediately alarms the experimenter to reduce the probability of the experiment failure. At the same time, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding errors. The resulting experiment failed.
  • the invention can provide the required pre-cooling environment (2-8 ° C) for the reagent by setting the sounding device, the control unit and the battery in the body of the reagent box and the refrigeration device and the temperature control probe on the outer wall of the reagent hole, and can pass the temperature control When the probe detects the temperature, once the setting range is exceeded, the sounding device immediately alarms the experimenter to reduce the probability of the experiment failure. At the same time, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding errors. The resulting experiment failed.

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  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne un kit de réactif ayant une fonction d'isolation à froid, une fonction d'alarme et une fonction d'alerte d'addition d'échantillon, comprenant un corps (10) de kit et un couvercle (20) de kit, le corps (10) de kit étant pourvu d'un dispositif sonore (40), d'une unité de commande, d'une batterie de stockage miniaturisée, d'un capteur (60), d'une ouverture (32) de collecte de tête de pointe de pipette, d'un disque de rotation (36) de réactif et d'un mécanisme d'alimentation. Par la disposition du dispositif sonore (40), de l'unité de commande et de la batterie de stockage sur le corps (10) de kit et la disposition d'un dispositif de réfrigération (381) et d'une sonde (382) de régulation de température sur une paroi extérieure d'une ouverture de réactif, on peut obtenir un environnement de pré-refroidissement requis (2-8°C) pour un réactif et la température peut être détectée via la sonde (382) de régulation de température et lorsque la température dépasse une plage définie, le dispositif sonore (40) émet immédiatement une alarme pour alerter le personnel chargé de l'expérience du dispositif, réduisant ainsi le taux de défaillance d'une expérience. De plus, au moyen du disque de rotation (36) de réactif entraînant la rotation de l'orifice de réactif, le personnel chargé de l'expérience peut être alerté pendant l'addition d'échantillon, ce qui évite une défaillance d'expérience provoquée par une addition incorrecte d'échantillon.
PCT/CN2016/085917 2016-07-01 2016-07-01 Kit de réactif présentant une fonction d'isolation à froid, une fonction d'alarme et une fonction d'alerte d'addition d'échantillon Ceased WO2018000110A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/085917 WO2018000110A1 (fr) 2016-07-01 2016-07-01 Kit de réactif présentant une fonction d'isolation à froid, une fonction d'alarme et une fonction d'alerte d'addition d'échantillon

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/085917 WO2018000110A1 (fr) 2016-07-01 2016-07-01 Kit de réactif présentant une fonction d'isolation à froid, une fonction d'alarme et une fonction d'alerte d'addition d'échantillon

Publications (1)

Publication Number Publication Date
WO2018000110A1 true WO2018000110A1 (fr) 2018-01-04

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PCT/CN2016/085917 Ceased WO2018000110A1 (fr) 2016-07-01 2016-07-01 Kit de réactif présentant une fonction d'isolation à froid, une fonction d'alarme et une fonction d'alerte d'addition d'échantillon

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WO (1) WO2018000110A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115248327A (zh) * 2021-04-26 2022-10-28 深圳市帝迈生物技术有限公司 Poct血细胞分析仪、试剂盒
CN115992039A (zh) * 2023-03-21 2023-04-21 深圳市美德瑞生物科技有限公司 一种下部磁吸自动核酸提取装置

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007106833A2 (fr) * 2006-03-13 2007-09-20 Sage Science, Inc. Reactif de laboratoire et ensemble echantillon, gestion et traitement
CN204489596U (zh) * 2015-01-23 2015-07-22 新疆维吾尔自治区产品质量监督检验研究院 一种便携式温控试剂箱
CN204989193U (zh) * 2015-07-16 2016-01-20 武汉塞力斯生物技术有限公司 一种类风湿因子测定试剂盒
CN205049580U (zh) * 2015-09-08 2016-02-24 天津金虹生物科技开发有限公司 一种高尔基体蛋白gp73的定量测定试剂盒
CN205329041U (zh) * 2015-12-17 2016-06-22 深圳精准医疗科技有限公司 慢病毒试剂盒的盒子

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007106833A2 (fr) * 2006-03-13 2007-09-20 Sage Science, Inc. Reactif de laboratoire et ensemble echantillon, gestion et traitement
CN204489596U (zh) * 2015-01-23 2015-07-22 新疆维吾尔自治区产品质量监督检验研究院 一种便携式温控试剂箱
CN204989193U (zh) * 2015-07-16 2016-01-20 武汉塞力斯生物技术有限公司 一种类风湿因子测定试剂盒
CN205049580U (zh) * 2015-09-08 2016-02-24 天津金虹生物科技开发有限公司 一种高尔基体蛋白gp73的定量测定试剂盒
CN205329041U (zh) * 2015-12-17 2016-06-22 深圳精准医疗科技有限公司 慢病毒试剂盒的盒子

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115248327A (zh) * 2021-04-26 2022-10-28 深圳市帝迈生物技术有限公司 Poct血细胞分析仪、试剂盒
CN115992039A (zh) * 2023-03-21 2023-04-21 深圳市美德瑞生物科技有限公司 一种下部磁吸自动核酸提取装置

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