WO2018080039A1 - Composition for preventing hair loss or improving hair growth, containing yellow-colored soybean leaf extract - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss or improving hair growth, containing a yellow-colored soybean leaf extract and, more specifically, to a composition having an effect of preventing hair loss or improving hair growth by containing an extract extracted from soybean leaves having turned yellow during soybean growth. According to the present invention, when the composition containing a yellow-colored soybean leaf extract is used, there is an effect of preventing hair loss or improving hair growth by promoting the activity of the Wnt/β-catenin signaling system in tissue, associated with hair loss, such as dermal papilla cells.

Description

Hair loss prevention or hair growth composition containing maple leaf extract

The present invention relates to a composition for preventing hair loss or hair growth containing maple leaf extract, and more specifically, by containing an extract extracted from soybean leaves in which the color of the bean leaf has changed yellow during the growth of soybean. It relates to a composition.

The scalp is composed of epidermis, dermis and subcutaneous tissue. The outermost epidermis functions to protect sensitive tissues of the body from damage. However, environmental factors affecting the scalp, such as changes in temperature, humidity, and stress and lack of influence due to external stimuli such as exposure to pollutants, can lead to loss of scalp function and affect hair loss. .

Tissues associated with hair loss include sebaceous gland, keratinocytes of the epidermis and hair follicles, dermal papilla cells (dermal papilla cells), sweat gland, and root sheath of the scalp hair. foll icle).

In male expression, testosterone is involved in skeletal muscle increase and sperm formation, and dihydrotestosterone (DHT) is known to be involved in tissues such as acne, sebum increase, hair loss and prostatic hyperplasia (DHT). Thiboutot et al., J. Invest.Dermatol., 105 (2): 209-14, 1995).

Androgenetic alopecia is caused by a male hormone called testosterone, which is converted into a more powerful hormone, dihydrotestosterone, by an enzyme called 5-alpha reductase (5 alpha-reductase). It acts on the hair follicles and induces hair follicles from the growth stage to the degenerative stage and causes hair loss. Therefore, a method of inhibiting the production of DHT by 5 alpha-reductase is mainly used to treat androgenetic alopecia.

Meanwhile, the 5-alpha reductase inhibitor finasteride has been shown to improve hair loss by reducing the hair loss hormone DHT in alopecia patients (Van Neste et al. , 2000) . As an important target for hair loss treatment, it has been used to develop hair loss treatments. Researchers looking for pharmacologically active substances to treat hair loss by inhibiting the activity of 5-alpha reductase, a biological enzyme involved in the reaction of dihydrotestosterone in testosterone, have been studied. A lot is done.

Gynecologic alopecia is mainly caused by a decrease in the amount of estrogen after menopause. Minoxidil or estrogen is mainly used as a treatment for female alopecia. Alopecia areata is caused by autoimmune diseases, mental stress, or genetic predisposition. This alopecia areata is fundamentally different from androgenetic alopecia, and the treatment is also different, using a method of treating corticosteroids, or applying minoxidil to the affected area or artificially causing irritation in the affected area.

As for the causes of complex hair loss, ingredients for the purpose of promoting blood circulation, inhibiting the action of testosterone, and strengthening the hair root function, etc. are sold as products on the market, but there are still no clear effects and problems of side effects. May be raised. For example, minoxidil has been reported to cause sticky feeling and irritation to the skin. Finasteride is currently used as an oral preparation, but side effects such as sexual dysfunction have been reported according to its intake. The effect can be expected only by oral administration, there was a lot of inconvenience in use.

For the prevention and treatment of hair loss, hormone replacement therapy is used as the most effective method. However, in the case of long-term administration of hormones such as estrogen, there is a risk of carcinogenesis such as breast cancer and uterine cancer, and there are problems such as side effects such as headache and weight gain.

Meanwhile, isoflavone of soybean, an phytoestrogen extracted from plants, is commonly used for hair loss improvement, but issues regarding effects and safety have been continuously raised. Therefore, there is an urgent need for the development of a hair loss treatment agent using a safer and more effective natural derived material.

Selective estrogen receptor modulators (SERMs) are selective estrogen receptor modulators, which are substances that bind to estrogen receptors and act as estrogen agonists in some tissues and as estrogen antagonists in other tissues. By using the characteristics of SERM, development of SERM-containing drugs that are safe in tissues such as uterus and breast, which are highly likely to be carcinogenic, and effective in improving menopausal syndrome are being progressed.

Apigenin is a plant polyphenol that is widely distributed in fruits and vegetables, especially in parsley, celery, garlic and cabbage, and is known to have anti-inflammatory, anti-cancer and free radical scavenging properties. Kim et al., Prostag . Leukotr . Ess . , 58: 17-24, 1998).

Coumestrol is known to be extremely trace or rarely present in soy or legumes. Compared to isoflavones, cumestrol's estrogen activity is 30 to 100 times higher, which is very useful in relieving uterine cancer, bone marrow cancer, breast cancer or brain cancer and menopausal symptoms.

Caffeine stimulates the metabolism of dermal papilla cells to promote hair follicle growth (Kren et al, Zygote, 12 (1): 31-38, 2004; Tsianakas A et al., Arch. Dermatol . Res., 296: 450 , 2005), which has been reported to inhibit the action of dihydrotestosterone, an active male hormone that shrinks hair follicles (Fischer et al., Int J Dermatol. , 46 (1): 27-35, 2007). However, too much caffeine may affect the dermal papilla cells and cause cell death (Hashimoto et al., Cancer Res. , 64 (9): 3344-3349, 2004). There is also research. In other words, if caffeine reaches hair follicles in an amount sufficient to prevent hair loss and hair growth, 60 cups of caffeine-containing coffee or tea should be drunk daily, or a high concentration of caffeine can be applied to the scalp. Due to the high efficacy and safe hair loss prevention agent development is insufficient.

Ginseng (人蔘, Panax ginseng CA Mey. 1909) is a perennial herb that belongs to the arboraceae family and is used as a medicinal herb. The efficacy of ginseng on the active ingredient began to be revealed by Brekhman (1957) as a study on ginsenosides or saponins. Ginsenosides are known to have many effects such as cancer cell proliferation inhibitory effect, hypertension improvement, antioxidant effect, antistress and the like.

Green tea has been a perennial evergreen plant (eg Camellia sinensis ) belonging to the tea tree family, and has been drinking since the Three Kingdoms period.In recent years, its value has been recognised as the functionalities of green tea are gradually revealed. have. Green tea contains high amounts of useful ingredients such as polyphenols (eg catechins), caffeine, amino acids, and vitamin C. It has been shown to be excellent for hangover and nicotine detoxification, fatigue recovery, cardiovascular action and skin beauty. Antioxidant action, anti-cholesterol action, hypoglycemic action, anti-tumor action, and platelet aggregation inhibitory effects on polyphenols, which are contained the most, have been attracting much attention.

Catechin, the main physiologically active substance of green tea, is a polyphenol containing about 100 mg in a cup of green tea, mainly epigallocatechin gallate (hereinafter referred to as "EGCG") and epicatechin gallate (epicatechin gallate). Gallate catechin (hereinafter referred to as "GC"), other epicatechin (hereinafter referred to as "EC") and epigallocatechin (hereinafter referred to as "ECC"), and epigallocatechin (hereinafter referred to as "EGC"). It is composed of various kinds of catechins, and these ingredients are food materials having various bioregulatory functions such as anti-caries, antibacterial, antioxidant, deodorant action, blood glucose level and blood cholesterol elevation suppression, anti-tumor, and lipid peroxide production inhibitory action. As a practical application, it is widely used in the field of processed foods. These catechins are known to have three to four times the antioxidant effect of vitamin C and vitamin E.

In addition, catechins are water-soluble, but tea leaves also contain fat-soluble components such as vitamin E and β-carotene. These components are used to remove free radicals and active oxygen-derived products that adversely affect immune response or cancer in vivo. It is known to be involved, and at the same time, it can be expected that synergistic effects can be expected when ingested with catechins, which also have antioxidant activity.

Wnt / β-catenin signaling system promotes hair follicle formation (Andl T, et al ., Dev Cell, 2 (5): 643-653, 2002) and maintains and activates genes that are expressed during the growth phase of the hair cycle. Plays an important role (Kishimoto J, et al., Genes & Dev , 14: 1181-1185, 2000) and promotes the differentiation of stem cells from keratinocytes (Huelsken J, et al., Cell 105 (4) : 533-545, 2001).

The activity of the Wnt / β-catenin signaling system is known to act on skin production and hair follicle development, and hair growth is inhibited in mice knocked down the Wnt gene. Thus, substances that promote the activation of the Wnt / β-catenin signaling system present in hair follicle stem cells can promote hair growth.

Minoxidil is one of the hair growth promoting drugs approved by the US Food and Drug Administration (FDA) .It was originally developed as a vasodilator for the treatment of hypertension. It is becoming. The specific mechanism of the minoxidil's hair growth effect is not yet clear, but K _ATP -channel opening, increased nutrient supply through vasodilation and activation of β-catenin pathway in human dermal papilla cells The back is thought to induce hair growth. Human dermal papilla cells are mesoderm-derived fibroblasts, which produce hair cycles through the secretion of proteins such as insulin-like growth factor-1 (IGF-1), hepatocyte growth factor (HGF), and fibroblast growth factor-7 (FGF-7). Minoxidil is known to have an effect of proliferating the growth of human dermal papilla cells through lowering of apoptosis of human dermal papilla cells.

Therefore, measuring the effects of inhibiting 5-alpha reductase activity, NIH3T3 fibroblast proliferation, and growth proliferation of human dermal papilla cells of any substance will help to effectively treat hair loss and improve hair growth. It can be a valid indicator to assess whether it can.

On the other hand, according to the prior art, Patent Document 1 below discloses the skin inflammation, allergy and atopic improvement effect and skin moisturizing effect of the composition comprising a soybean leaf extract, Patent Document 2 below contains a composition containing a ginseng extract and green tea extract Efficacy of inhibiting hair loss, hair loss and dandruff of the invention is disclosed, but containing the extract extracted from the soybean leaves (ie, maple soybean) that the color of the soybean leaves turned yellow during the growth of the beans as an active ingredient, the composition for promoting hair growth and improving hair loss symptoms Is not disclosed.

Under these technical backgrounds, the present inventors have developed a composition showing the effect of promoting hair growth and improving hair loss by containing an extract extracted from the soybean leaf extract, ie, the soybean leaf whose color has changed yellow during soybean growth. It was.

[Preceding technical literature]

[Patent Documents]

(Patent Document 1) Korean Unexamined Patent Publication No. 10-2014-0034964 (2014.03.21.)

(Patent Document 2) Korean Unexamined Patent Publication No. 10-2015-0030095 (2015.03.19.)

Accordingly, the present inventors have made diligent efforts to develop a composition containing a plant-derived SERM (phytoSERM) effective for preventing hair loss or improving hair growth, and thus extracted from a bean leaf (ie, maple leaf) in which the color of the bean leaf has changed yellow during the growth of the bean. It was confirmed that the extract exhibits the effect of promoting hair growth and improving hair loss symptoms by promoting the activity of the Wnt / β-catenin signaling system of dermal papilla cells, thereby completing the present invention.

Therefore, the main purpose of the present invention is to provide a composition containing as an active ingredient maple leaf extract excellent in promoting hair growth and preventing hair loss.

Another object of the present invention is a composition excellent in promoting hair growth and preventing hair loss, comprising mixing at least one substance selected from the group consisting of apigenin, cumestrol, caffeine, ginseng extract and green tea extract with maple leaf extract It is to provide a composition containing a mixture as an active ingredient.

In order to achieve the above object, the present invention is a composition for preventing hair loss or hair growth containing maple leaf extract as an active ingredient, preferably a pharmaceutical composition for hair loss prevention or hair growth containing maple leaf extract as an active ingredient, health function Provided are food compositions and cosmetic compositions.

The present invention also prevents hair loss or improves hair growth, which contains a mixture of at least one substance selected from the group consisting of apigenin, cumestrol, caffeine, ginseng extract, and green tea extract as an active ingredient. It provides a composition for preventing hair loss or improving hair growth, preferably a nutraceutical composition and cosmetic composition containing the mixture as an active ingredient.

The present invention also provides the use of maple leaf extract as a hair loss preventing or hair growth improving agent in the manufacture of a pharmaceutical composition or medicine for preventing hair loss or hair growth.

The present invention also, in the manufacture of a pharmaceutical composition or medicament for preventing hair loss or hair growth, at least any one selected from the group consisting of apigenin, cumestrol, caffeine, ginseng extract and green tea extract and maple leaf It provides a use of the mixture of the extract as a hair loss preventing agent or hair growth improving agent.

The present invention also provides a use of maple leaf extract as a hair loss preventing agent or hair growth improving agent in the manufacture of a health food composition for preventing hair loss or hair growth.

The present invention also, in the production of a health food composition for preventing hair loss or hair growth, apigenin, cumestrol, caffeine, ginseng extract and green tea extract and at least one selected from the group consisting of maple leaf extract It provides a use of the mixture of the mixture as a hair loss preventing agent or hair growth improving agent.

The present invention also provides the use of maple leaf extract as a hair loss preventing agent or hair growth improving agent in the production of a cosmetic composition for preventing hair loss or hair growth.

The present invention also, in the manufacture of a cosmetic composition for preventing hair loss or hair growth, mixing at least one substance selected from the group consisting of apigenin, cumestrol, caffeine, ginseng extract and green tea extract and maple leaf extract Provided is the use of a mixture as a hair loss preventing agent or hair growth improving agent.

When the composition containing the maple leaf extract according to the present invention is used, hair loss prevention or hair growth improvement effect is achieved by promoting the activity of the Wnt / β-catenin signaling system in tissues associated with hair loss, such as dermal papilla cells.

1 is a graph showing the results of HPLC analysis of soybean leaf extract for each growth stage of soybean (bean leaf).

Figure 2 is a graph showing the HPLC analysis results of the maple leaf extract and soybean extract according to the present invention.

Figure 3 is a graph showing the degree of response according to the activity of the Wnt / β-catenin signaling system (Wnt / β-catenin signaling cascade) of the human dermal papilla cells treated with maple bean leaf extract or soybean extract according to the present invention.

Figure 4 is a graph showing the hair growth length of the human hair follicle organ treated with maple leaf extract or soybean extract according to the present invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art.

The term "about" as used herein to express volume, time (period), concentration, content, area, etc., means that there is a maximum of 10% tolerance within that value or range of values.

In the present specification, the "foliage bean leaf" refers to the bean leaf color of the bean leaf yellow during the growth of the beans, such beans leaf during the growth stage of the beans 1) soybean pods and soybean turns yellow (time R7); From 2) with the leaves falling soybean pods and beans completely yellow (time R8); It means a bean leaf in. Specifically, the "foliage bean leaf" refers to a bean leaf in which a green or green bean leaf is in a yellow state (that is, a bean leaf in a partially yellow state) or a bean leaf in a completely yellow state. The soybean leaves in the state where the soybean leaves are turned yellow are about 1/10 or more, preferably about 1/5 or more, more preferably about 1/3 or more, and particularly more preferably about 1/10 or more based on the total area of the soybean leaves. More than one half, most preferably about four fifths being yellow.

In an aspect of the present invention, maple bean leaf extract may be extracted from the soybean leaves of R7 to R8 step of the growth of the following beans. In this specification, the growth stage of the beans is as follows.

VE stage: 1 to 2 weeks after planting seeds, the cotyledon rises from the soil

VC stage: cotyledon blooms, a word grows over it, and leaves

Step V1: A word is produced from the first outer leaf, resulting in three leaves

Stage V2: In the stage V1, one more word is created, resulting in three leaves

Phase V3: In the V2 phase, one more word is generated, resulting in three leaves

V4 stage: In the V3 stage, one more word is created, creating three leaves

V5 stage: An additional word in V4 stage creates 3 leaves

R2 stage: Beanweed in full bloom

Step R4: Completed pod creation

Step R5: Soybeans inside the pod

Step R6: Green Beans Completed in the Pods

R7 stage: Soybean pods and beans turning yellow

R8 stage: Soybean pods and soybeans are completely yellow with leaves falling

In one embodiment of the present invention, the activity change of the Wnt / β-catenin signaling system of the hair follicle stem cells according to the maple leaf extract treatment was measured. As a result, as shown in Figure 3, when treated with maple leaf extract (Example 4) at a concentration of 10ppm or more, 150% or more Wnt / β-catenin luciferase reaction degree (that is, activity of the Wnt / β-catenin signaling system ), And the degree of reaction increased depending on the concentration of the maple leaf extract (Example 4). Therefore, the maple leaf extract was found to have a hair growth promoting effect by promoting the activity of the Wnt / β-catenin signaling system, a hair growth promoting signal of hair follicle stem cells (see Test Example 2).

In another embodiment of the present invention, the hair growth effect of the human hair follicle organ according to the maple leaf extract treatment was confirmed. As a result, as shown in Figure 4, the maple leaf extract (Example 4) showed a hair growth effect equivalent to or greater than the positive control group in the concentration range of 1, 10 and 50ppm, especially hair length at 10ppm concentration Growth was the best. Therefore, it was confirmed that the maple leaf extract is effective in growing hair of human hair follicle organs (see Test Example 3).

In another embodiment of the present invention, in the treatment of the maple leaf extract or a mixture comprising any one substance selected from the group consisting of apigenin, cumestrol, caffeine, ginseng extract and green tea extract and maple leaf extract The proliferative effect of human dermal papilla cells was confirmed. As a result, as shown in Table 2, compared to human hair papilla cells treated with DMSO (negative control), human hair papilla cells treated with 1, 10 or 50 ppm maple leaf extract (Example 4) increased cell proliferation rate. In particular, the cell proliferation rate of human dermal papilla cells treated with 10 ppm maple leaf extract (Example 4) was the highest, indicating that they had the same level of cell proliferation effect as the human dermal papilla cells (positive control) treated with minoxidil. Confirmed. In addition, any one selected from the group consisting of apigenin, cumestrol, caffeine, ginseng extract (Comparative Example 1) and green tea extract (Comparative Example 2) and 10ppm maple leaf extract (Example 4) Treatment of one mixture with human dermal papilla cells resulted in a significant increase in cell proliferation of human dermal papilla cells. Therefore, it was confirmed that there is a synergistic effect of further promoting the hair growth effect when the maple bean leaf extract and the substance are mixed and treated to human hair papilla cells (see Test Example 4).

Substances that promote hair growth through the regulation of the activity of the Wnt / β-catenin signaling system are not involved in promoting hair growth through the regulation of testosterone and / or blood circulation, thus inhibiting testosterone or promoting blood circulation. The method will improve severe hair loss and gynecomastia that have not been resolved.

Accordingly, the present invention relates to a hair loss preventing or hair growth pharmaceutical composition, health functional food composition or cosmetic composition containing maple leaf extract as an active ingredient in one aspect, wherein the maple leaf extract is preferably a part or The whole may be extracted from the soybean leaves when turned yellow, and more preferably may be characterized in that extracted from the soybean leaves of R7 to R8 stage of the growth stage of the beans.

In another aspect, the present invention is to prevent hair loss comprising at least one selected from the group consisting of apigenin (apigenin), cumestrol (Coumestrol), caffeine, ginseng extract and green tea extract and maple leaf extract in a specific mixing ratio or It relates to a composition for improving hair growth.

In the present invention, the 'bean' is not limited in kind, for example, Seoritae ( Glycin max MERR), Seomoktae (Seomoktae, Rhynchosia Nolubilis ), Black soybean, Glycine max (L.) Merr., Blue bean, Glycime max MERR, Yellow bean, Glycime max MERR, Field bean, Vicia faba ), Kidney bean ( Phaseolus) vulgaris ), pinto bean, Phaseolus vulgaris L., small red bean, Vigna angularis ), small black bean, Phaseolus angularis WF WIGHT., bean sprouts (sprouting bean, Glycine max (L.) Merr.) and soybeans (soybean, Glycine max ) It is not limited to this.

In one aspect of the present invention, the "foliage leaf extract" includes any material obtained by extracting the components of the maple leaf, regardless of the extraction method, extraction solvent, extracted component or form of the extract and extract the components It includes the material obtained by the extraction method including the process of treatment with heat, acid, base, enzyme, etc. in the process, and extracts the material obtained by extracting the components of the maple leaf and then processed by another method or It is a broad concept that includes all of the materials that can be obtained by treatment. Specifically, the processing or treatment may be to perform fermentation or enzyme treatment, such as maple leaf extract. Thus, in one aspect of the invention, the maple bean leaf extract may be a fermentation product.

In one aspect of the present invention, the "foliage bean leaf" may be in the form of an extract, or raw (bean) raw maple leaf, the pulverized product of the herbal medicine, dried product of the herbal medicine, dry ground product of the herbal medicine, fermented product of the maple bean leaf, but It is not limited. In addition, the maple bean leaf used in the present specification is not limited to the acquisition method, may be grown and used or purchased commercially available, it may be used part or all of the ground or root portion of the herb. More specifically, one or more selected from the group consisting of leaves, stems, roots, and flowers of maple leaf herb may be used, and more specifically, flowers, leaves, and stems may be used. In one aspect of the present invention, the maple bean leaf is not necessarily produced through drying and is not limited as long as it is a raw material of a form suitable for extracting the active ingredient of the maple bean leaf.

In one aspect of the invention, the maple leaf extract may be one or more extracts selected from the group consisting of water, organic solvents and mixtures thereof.

In one aspect of the invention, the water comprises distilled or purified water, the organic solvent is one or more selected from the group consisting of C1 ~ C6 lower alcohol, acetone, ether, ethyl acetate, diethyl ether, ethyl methyl ketone and chloroform It includes, but is not limited to. Specifically, the alcohol may be methanol or ethanol.

As used herein, the term "improve" or "alleviate" refers to the reduction or elimination of conditions, diseases, disorders or phenotypes, including abnormalities or symptoms, syndromes. Here, improving or alleviating hair loss refers to preventing, ameliorating or alleviating alopecia diseases by promoting the activity of the Wnt / β-catenin signaling system.

As used herein, "treatment" refers to any activity in which the symptoms of alopecia disease improve or cure, and more specifically, to reverse the disease or condition to which the term applies, or one or more symptoms of the disease or condition. Means to mitigate, mitigate, inhibit, or prevent the progression of.

Maple bean leaf extract of the present invention may be characterized by containing a phyto-SERM (phyto-selective estrogen receptor modulator). phyto-SERM is a combination of "phyto" meaning plant-derived and "selective estrogen receptor modulator" (SERM). Selective estrogen receptor modulators refer to substances that bind to the estrogen receptor and act as estrogen agonists in some tissues and as estrogen antagonists in other tissues. The composition containing the soybean leaf extract of the present invention acts as an antagonist to estrogen receptors in tissues such as the uterus and breast, which is highly likely to be carcinogenic, while lowering the risk of carcinogenesis such as uterine cancer and breast cancer, and more preferably the skin, preferably the scalp, more preferably. Hair follicles are expected to contain phyto-SERM, which acts as an agent on human dermal papilla cells and is effective for promoting hair growth and preventing hair loss.

In the present invention, the maple leaf extract may be characterized in that it contains 1 to 60% by weight, preferably 5 to 50% by weight, more preferably 10 to 30% by weight based on the total weight of the composition have. If the content of the maple leaf extract is less than 1% by weight, it is difficult to expect hair growth promoting and hair loss prevention effects, and when the content of the content exceeds 60% by weight, the stability of the formulation is lowered, which affects the component stability of the extract. It is expected.

In the present invention, the composition may further comprise at least one selected from the group consisting of apigenin (apigenin), cumestrol (Coumestrol), caffeine, ginseng extract and green tea extract.

When the composition contains a mixture of maple leaf extract and apigenin, the mixture is 1-10: 1-10, preferably 1-2: 1-2, of maple leaf extract and apigenin on a ppm concentration basis. More preferably, it may contain a mixing ratio of 2: 1.

When the composition contains a mixture of maple leaf extract and cumestrol, the mixture is 1-10: 1-10, preferably 1-2: 1 ~ maple leaf extract and cumestrol on a ppm concentration basis. 2, More preferably, it is characterized by containing in a mixing ratio of 2: 1.

When the composition contains a mixture of maple leaf extract and caffeine, the mixture is 1-10: 1-10, preferably 1-2: 1-2, more preferably, on a ppm concentration basis, maple leaf extract and caffeine It may be characterized by containing in a mixing ratio of 2: 1.

When the composition contains a mixture of maple leaf extract and ginseng extract, the mixture is based on the ppm concentration, the maple leaf extract and ginseng extract 1 ~ 10: 1 ~ 10, preferably 1 ~ 2: 1 ~ 2, More preferably, it may contain a mixing ratio of 2: 1.

When the composition contains a mixture of maple leaf extract and green tea extract, the mixture is based on the ppm concentration, 1 ~ 10: 1 ~ 10, preferably 1 ~ 2: 1 ~ 2, More preferably, it may contain a mixing ratio of 2: 1.

The mixture of the maple leaf extract and apigenin, the mixture of maple leaf extract and cumestrol, the mixture of maple leaf extract and caffeine, the mixture of maple leaf extract and ginseng extract, or the mixture of maple leaf extract and green tea extract are in the ppm concentration. A mixture of apigenin, cumestrol, caffeine, ginseng extract and green tea extract may be mixed with a mixture ratio of the corresponding weight ratio (wt%) and maple bean leaf extract.

In the present invention, the soybean leaf extract may promote the activity of the Wnt / β-catenin signaling system, preferably promote the activity of the Wnt / β-catenin signaling system of dermal papilla cells, More preferably, it promotes the activity of the Wnt / β-catenin signaling system of human dermal papilla cells.

In the present invention, the bean leaf extract may be characterized by growing hair of the hair follicle organ.

In the present invention, the soybean leaf extract may be characterized by having the effect of promoting hair growth and / or hair loss symptoms by promoting the activity of the Wnt / β-catenin signaling system of the dermal papilla cells.

The pharmaceutical composition containing the maple leaf extract of the present invention is a preservative, stabilizer, hydrating or emulsifying accelerator, pharmaceutical adjuvants such as salts and / or buffers for the control of osmotic pressure and other therapeutically useful substances (carriers, excipients, diluents, etc.) ), And may be formulated in various oral or parenteral dosage forms according to conventional methods.

The term "carrier" is defined as a compound that facilitates the addition of a compound into a cell or tissue. For example, dimethyl sulfoxide (DMSO) is a commonly used carrier that facilitates the incorporation of many organic compounds into cells or tissues of an organism.

The term "excipient" is a substance that is added to make it easier to take medicine or to have a certain color and form when the amount of the main medicine is small in the process of preparation of tablets or pills, such as lactose or starch. do.

The term "diluent" is defined as a compound that not only stabilizes the biologically active form of the compound of interest, but also is diluted in water to dissolve the compound. Salts dissolved in buffer solutions are used as diluents in the art. A commonly used buffer solution is phosphate buffered saline, because it mimics the salt state of human solutions. Because buffer salts can control the pH of a solution at low concentrations, buffer diluents rarely modify the biological activity of a compound.

Compositions containing the Maple Leaf extract as used herein may be administered to a human patient as such or as a pharmaceutical composition mixed with other active ingredients as in combination therapy or with a suitable carrier or excipient.

Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The oral dosage forms include, for example, tablets, pills, hard and soft capsules, liquids, suspensions, emulsifiers, syrups, powders, powders, fine granules, granules, pellets, and the like, and these formulations include surfactants in addition to active ingredients. , Diluents such as lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and glycine, and glidants such as silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycols. . Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally starch, agar, alginic acid or its sodium salt Pharmaceutical additives such as disintegrants, absorbents, colorants, flavors, and sweeteners. The tablets can be prepared by conventional mixing, granulating or coating methods.

In addition, the parenteral administration agent may be, for example, formulations such as injections, drops, ointments, lotions, gels, creams, sprays, suspensions, emulsions, suppositories, patches, and the like. It is not.

The pharmaceutical composition according to an embodiment of the present invention may be administered orally, parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous. Pharmaceutical compositions according to one embodiment of the invention may be administered topically to the scalp, for example.

In addition, the pharmaceutically acceptable dose, ie dosage, of the active ingredient depends on the age, sex, and weight of the subject to be treated, the specific disease or pathology to be treated, the severity of the disease or pathology, the route of administration and the judgment of the prescriber Will be different. Dosage determination based on these factors is within the level of skill in the art. The dosage is, for example, 1 to several times so as to be administered in an amount of 0.01 to 5000 mg, preferably 0.1 to 2000 mg, more preferably 0.5 to 500 mg, and most preferably 1 to 100 mg per kg of body weight per day. It can be administered in divided, but the dosage is not intended to limit the scope of the invention in any way.

Soybean leaf extract or maple soybean leaf extract of the present invention is a natural substance so there is no toxicity at all can be continuously used as a pharmaceutical.

Pharmaceutical compositions suitable for use in the present invention include compositions in which the active ingredients, including maple leaf extract, are contained in an amount effective to achieve their intended purpose. More specifically, a therapeutically effective amount means an amount of a compound effective to prolong the survival of the subject to be treated or to prevent, alleviate or alleviate the symptoms of a disease. Determination of a therapeutically effective amount is within the capabilities of those skilled in the art, in particular in terms of the detailed disclosure provided herein.

A therapeutically effective amount for maple leaf extract used in the methods of the invention, and compositions (compounds) containing the same as an active ingredient, can be measured initially from cell culture assays. For example, dose can be calculated in an animal model to obtain a range of circulating concentrations that includes an IC ₅₀ (half maximal inhibitory concentration) or EC ₅₀ (half maximal effective concentration) determined in cell culture. Such information can be used to more accurately determine useful doses in humans.

Toxicity and therapeutic efficiency of the Maple Leaf Extract described herein, or compositions (compounds) containing it as an active ingredient, are described, for example, LD ₅₀ (fatal to 50% of the population), ED ₅₀ ( ₅₀ of the population). To determine the dose with a therapeutic effect on%), IC ₅₀ (the dose with a therapeutic inhibitory effect on 50% of the population), it can be estimated by means of surface pharmaceutical procedures in cell culture or laboratory animals. The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio between LD ₅₀ and ED ₅₀ (or IC ₅₀ ). Compounds showing high therapeutic indices are preferred. The data obtained from these cell culture assays can be used to estimate the range of doses used in humans. The dosage or dosage of such compounds is preferably in the range of circulating concentrations including ED ₅₀ (or IC ₅₀ ) in the absence or little toxicity.

"Functional food" or "functional food" in the present invention means a food that improves the functionality of the general food by adding the maple bean leaf extract of the present invention to the general food.

Functionality can be roughly divided into physical properties and physiological functions. When the extract of the present invention is added to general foods, the physical properties and physiological functions of general foods will be improved, and the present invention provides a food product of such an improved function as a comprehensive 'functional food. (Health functional food) "or" functional food (health functional food) ".

In the present invention, "health functional food" refers to a food manufactured and processed using raw materials or ingredients having functional properties useful for the human body according to the Health Functional Food Act No. 10213, and nutrients for the structure and function of the human body. It is meant to be consumed for the purpose of regulating or obtaining a useful effect for health use such as physiological action.

The health functional food containing the maple bean leaf extract of the present invention may contain other ingredients and the like which can give a synergistic effect to the main effect within a range that does not impair the main effect of the present invention. For example, it may further include additives such as perfumes, pigments, fungicides, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, emulsifiers and synthetic polymer materials to improve physical properties. In addition, supplementary ingredients such as water soluble vitamins, oil soluble vitamins, polymer peptides, polymer polysaccharides and seaweed extract may be further included. The components may be appropriately selected and blended by those skilled in the art according to the formulation or purpose of use, and the amount of the additives may be selected within a range that does not impair the object and effect of the present invention. In addition, the compositions of the present invention can be used in the form of additives for other foods.

The formulation of the health functional food containing the maple leaf extract of the present invention is not particularly limited, but is formulated in various forms such as tablets, granules, drinks, beverages, solutions, emulsions, viscous mixtures, tablets, powders, and the like. Can be. In addition, the health functional food administration may be administered by a variety of methods, such as simple drinking, injection, spray or squeeze method.

According to an embodiment of the present invention, the composition of the present invention may be formulated into a cosmetic formulation and used in various ways, such as hair cosmetics having a hair growth promoting effect.

The cosmetic composition containing the maple bean leaf extract of the present invention is not particularly limited in formulation, and may be appropriately selected as desired. For example, softening cream (skin lotion and milk lotion), nourishing cream, essence, nourishing cream, massage cream, pack, gel, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, Body Lotion, Body Cream, Body Oil, Body Essence, Hair Tonic, Hair Conditioner, Hair Essence, Hair Lotion, Hair Nutrition Lotion, Hair Shampoo, Hair Rinse, Hair Treatment, Hair Cream, Hair Nutrition Cream, Hair Moisture Cream, Hair Massage Cream, Hair Wax, Hair Aerosol, Hair Pack, Hair Nutrition Pack, Hair Soap, Hair Cleansing Foam, Hair Oil, Hair Dryer, Hair Preservative, Hair Dye, Hair Wave, Hair Decolorant, Hair Gel, Hair Glaze, Hairdressing Hair , Hair lacquer, hair moisturizer, hair mousse and hair spray may be prepared in any one or more formulations selected from the group consisting of, but is not limited thereto. Preferably hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nourishment cream, hair moisturizer cream, hair massage cream, hair wax, hair aerosol, Hair Pack, Hair Nutrition Pack, Hair Soap, Hair Cleansing Foam, Hair Oil, Hair Dryer, Hair Preservative, Hair Dye, Hair Wave Agent, Hair Bleach, Hair Gel, Hair Glaze, Hair Dresser, Hair Lacquer, Hair Moisturizer, Hair It may be formulated as a mousse or hairspray.

When the composition of the present invention is formulated in a cosmetic formulation, the composition of the present invention may include all components commonly used in cosmetics in addition to the extract, such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo It may comprise a component selected from the group consisting of lipid and seaweed extract.

The polymer peptide can be selected as long as it can be blended into cosmetics, but preferably collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be selected. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

As the polymer polysaccharide, any one can be selected as long as it can be blended into cosmetics, but preferably hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be selected. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

As the sphingolipid, any one can be selected as long as it can be blended into a cosmetic, but preferably ceramide, phytosphingosine, sphingosaccharide lipid and the like can be selected. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The cosmetic of the present invention may be blended with the above essential components, if necessary, with other ingredients normally blended into the cosmetic. At this time, the compounding ingredients include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavors, blood circulation accelerators , Cooling agent, limiting agent, purified water and the like can be used.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, 0.001-50 weight% based on the total weight of a composition. Preferably it is 0.01-5 weight%, More preferably, it is mix | blended in 0.01-3 weight%.

Ingredients included in the composition formulated in the cosmetic formulation of the present invention may include components commonly used in cosmetics in addition to the extract as an active ingredient, for example, stabilizers, solubilizers, vitamins, pigments and flavors and Such conventional adjuvants and carriers.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

[ Example  1-4] Preparation of Soybean Leaf Extract by Growth Stage

Soybean leaves (V5 stage) about 45 days after sowing Glycine max (L.) Merrill (National Species Registration No. 01-0003-134), and soybean leaves (R2 ~~ 78 days) Step 3), about 107 days of soybean leaves (R6 stage), about 139 days of soybean leaves (R8 stage) were washed with purified water, dried and then granulated. 100 g of each powdered soybean leaf was put in 1 liter of an ethanol aqueous solution, extracted at room temperature (25 ° C.) for about 24 hours, and filtered through a 300 mesh filter cloth. The filtrate was then concentrated to powder. Thus, the powder of the soybean leaf extract of Example V5 (Example 1), the soybean leaf extract of Example R2-3 (Example 2), the soybean leaf extract of Example R6 (Example 3), and the soybean leaf extract of Example R8 (Example 4) About 10.3 g of each was obtained.

[ Comparative example  1] Preparation of Ginseng Extract

Raw ginseng root was dried by daylight drying or hot air to prepare ginseng root drying material. About 3L of water was added to about 1 kg of the ginseng root dry material, and the mixture was extracted under reflux, filtered and concentrated under reduced pressure at 40 to 45 ° C. to obtain about 300 g of ginseng root extract.

[ Comparative example  2] Preparation of Green Tea Extract

To about 300g of green tea was added about 4.5L of water heated to a temperature of 85-95 ℃ and then treated for about 2-3 hours while maintaining the temperature, then filtered and the green tea extract at 65-75 ℃ under reduced pressure About 82.7 g were obtained.

[ Comparative example  3] Preparation of Soybean Extract

Xinhua beans, the same varieties as the beans used in the preparation of Examples 1-4, were washed with purified water, dried, and then semalized. About 100 g of the granulated soybean powder was added to 1 liter of an ethanol aqueous solution, and extracted at room temperature (25 ° C.) for about 24 hours, followed by filtration with a 300 mesh filter cloth. The filtrate was then concentrated to powder. Thus, about 10.3 g of a powder of soybean extract was obtained.

In addition, any one species selected from the group consisting of apigenin (Sigma, USA), cumestrol (Sigma, USA), caffeine (Sigma, USA), ginseng extract (Comparative Example 1) and green tea extract (Comparative Example 2) A mixture of a substance and maple leaf extract (Example 4) was prepared as in Examples 5 to 9 (see Table 2 below).

[ Example  5]

A mixture consisting of about 10 ppm maple leaf extract (Example 4) and about 5 ppm apigenin (ppm concentration basis, 2: 1) was prepared.

[ Example  6]

A mixture consisting of about 10 ppm maple leaf extract (Example 4) and about 5 ppm cumestrol (2: 1 based on ppm concentration) was prepared.

[ Example  7]

A mixture consisting of about 10 ppm maple leaf extract (Example 4) and about 5 ppm caffeine (based on ppm concentration, 2: 1) was prepared.

[ Example  8]

A mixture consisting of about 10 ppm maple leaf extract (Example 4) and about 5 ppm ginseng extract (2: 1 based on ppm concentration) was prepared.

[ Example  9]

A mixture consisting of about 10 ppm maple leaf extract (Example 4) and about 5 ppm green tea extract (ppm concentration basis, 2: 1) was prepared.

[ Test Example  1] Comparative Analysis of Components of Soybean Leaf Extract by Growth Stage

The components of the soybean leaf extract (Examples 1 to 4) and the soybean extract (comparative example 3) for each growth stage were analyzed. Each of the extracts was dissolved in 70% ethanol to make a 10,000 ppm solution, followed by component analysis (Waters, 2996 PDA detector) using HPLC (Waters, 2695 model). The stationary phase was Mightysil RP-18 GP 250-4.6 (5 μm) column of Kanto Chemical, and the mobile phase used the composition ratio shown in Table 1 below.

Minutes water Acetonitrile 0 95 5 65 62 38 70 38 62 75 95 5 80 95 5

As a result, as shown in the HPLC analysis results of Figure 1, about 139 days after sowing soybean leaf (R8 step) extract (Example 4), about 45 days after sowing soybean leaf (V5 step) extract (Example 1), a new peak not found in soybean leaves (R2 ~ 3 stage) extract (Example 2) about 78 days after sowing and soybean leaf (R6 stage) extract (Example 3) about 107 days after sowing ) Can be seen. Therefore, it could be confirmed that the composition of the bean leaf extract varies depending on the growth stage of the bean leaf.

In addition, as shown in the HPLC analysis results of Figure 2, about 139 days after sowing soybean leaf (R8 step) extract (Example 4) isoflavones and derivatives thereof compared to the soybean seed extract (Comparative Example 3) ( Daidzein, Genistein, Daidzin, Genistin, Malonyl-Daidzin, Malonyl-Genistin) were found to have different levels of content, in particular high content of cumestrol and other unknown components.

[ Test Example  2] of Hair Follicle Stem Cells Treated with Maple Bean Leaf Extract Wnt / β- Catenin  Change in activity of signaling system

Seeds of NIH3T3 (Wnt), a hair follicle stem cell expressing luciferase, a reporter protein capable of measuring activity changes of the Wnt / β-catenin signaling system, were seeded at 10000 cells / well in 96-well plates (Nunc, Wiesbaden, Germany). seeding) and incubated for about 24 hours under conditions of 5% CO ₂ , 37 ℃. The maple leaf extract (Example 4) was treated in 1, 10, or 50 ppm of concentration in NIH3T3 (Wnt) cells incubated as described above and then cultured for about 24 hours (negative control: DMSO (Dimethyl Sulfoxide); positive control: Lithium chloride (LiCl) at 50 mM; Comparative example: Soybean Leaf Extract. Then, 100 μL of ONE-Glo reagent (Promega, Madison, WI, USA) was added to each well of the 96 well plate and reacted for 3 minutes. After the reaction, NIH3T3 was prepared using a luminometer Victor (PerkinElmer, Waltham, Massachusetts, USA). Wnt / β-catenin luciferase response (activity of the Wnt / β-catenin signaling system) of (Wnt) cells was measured (Wnt / β-catenin luciferase assay).

As a result, as shown in Figure 3, when treated with maple leaf extract (Example 4) at a concentration of 10ppm or more, 150% or more Wnt / β-catenin luciferase reaction degree (that is, activity of the Wnt / β-catenin signaling system ), And the degree of reaction increased depending on the concentration of the maple leaf extract (Example 4). On the other hand, the general bean leaf extract of Comparative Example showed almost no Wnt / β-catenin luciferase reaction (generally, when the degree of reaction of Wnt / β-catenin luciferase exceeds 150%, it is judged to have a hair growth promoting effect).

Therefore, the maple leaf extract was found to have a hair growth promoting effect by promoting the activity of the Wnt / β-catenin signaling system, a hair growth promoting signal of hair follicle stem cells.

[ Test Example  3] Hair Growth Effect of Human Hair Follicle Organs Treated with Maple Bean Leaf Extract

Human hair follicle organs were separated from the remaining tissues after hair transplantation and William 'E medium (L-glutamine (2 mM), insulin (10 μg / mL), hydrocortisone (40 ng / mL), antibiotics (1% w / v), and antifungal agents (1% w / v) containing medium). After treating maple leaf extract (Example 4) at a concentration of 1, 10 or 50 ppm (negative control group: DMSO; positive control group: 50 μM minoxidil), the length of hair grown from hair follicle organs was measured on the 3rd and 6th day of culture. .

As a result, as shown in Figure 4, the maple leaf extract (Example 4) showed a hair growth effect equivalent to or greater than the positive control group in the concentration range of 1, 10 and 50ppm, especially hair length at 10ppm concentration Growth was the best.

Therefore, it was confirmed that the maple leaf extract is effective in growing hair of human hair follicle organs.

[ Test Example  4] human of maple leaf extract, and mixture containing same Breast milk  Proliferative effect of cells

Human dermal papilla cells were seeded at 5000 cells / well in 96-well plates, incubated for about 24 hours under conditions of 5% CO ₂ , 37 ° C, and then (i) maple leaf extract (Example 4: 1, 10 or 50 ppm), 0.1% DMSO (negative control), 50 μM minoxidil (positive control), respectively, to human dermal papilla cells, or (ii) apigenin, cumestrol, caffeine, ginseng extract (Comparative Example 1) or One test substance of green tea extract (Comparative Example 2) was mixed with maple leaf extract (Example 4: 10 ppm) at a concentration of 5 ppm, and treated with human dermal papilla cells, and cultured for about 72 hours, followed by MTT assay (3 Cell proliferation was confirmed by measuring the degree of cell proliferation by-(4,5-Dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazolium bromide tetrazolium reduction assay.

sample Cell proliferation rate (%) Standard Deviation(%) 0.1% DMSO (Voice Control) 100.0 3.4 50 μM minoxidil (positive control) 112.4 9.0 Example 4: 1 ppm  Maple Bean Leaf Extract 105.7 4.5 Example 4: 10 ppm  Maple Bean Leaf Extract 114.1 3.6 Example 4: 50ppm  Maple Bean Leaf Extract 107.9 11.4 5 ppm apigenin 112.2 5.8 5 ppm Kumestrol 109.6 4.4 5 ppm caffeine 127.6 2.5 Comparative Example 1: 5 ppm ginseng extract 124.9 8.7 Comparative Example 2: 5 ppm green tea extract 106.5 5.1 Example 5: 10 ppm  Maple Bean Leaf Extract + 5ppm Apigenin 131.1 6.4 Example 6: 10ppm  Maple Bean Leaf Extract + 5ppm Kumestrol 129.3 5.5 Example 7: 10ppm  Maple Bean Leaf Extract + 5ppm Caffeine 138.8 4.7 Example 8: 10ppm  Maple Bean Leaf Extract + 5ppm Ginseng Extract 140.2 10.4 Example 9: 10ppm Maple Leaf Extract + 5ppm Green Tea Extract 133.9 8.3

As a result, as shown in Table 2, compared to human hair papilla cells treated with DMSO (negative control), human hair papilla cells treated with 1, 10 or 50 ppm maple leaf extract (Example 4) increased cell proliferation rate. In particular, the cell proliferation rate of human dermal papilla cells treated with 10 ppm maple leaf extract (Example 4) was the highest, which was confirmed to have the same level of cell proliferation effect as human dermal papilla cells (positive control) treated with minoxidil. It was.

On the other hand, apigenin, cumestrol, caffeine, ginseng extract (Comparative Example 1) or green tea extract (Comparative Example 2) were treated to human dermal papilla cells at a concentration of 5 ppm, respectively, and the cell proliferation rate was increased.

Apigenin, cumestrol, caffeine, ginseng extract (Comparative Example 1) and green tea extract (Comparative Example 2) of any one selected from the group consisting of 5ppm each 10ppm maple leaf extract (Example 4) Treatment with human dermal papilla cells resulted in a significant increase in cell proliferation of human dermal papilla cells.

Therefore, it was confirmed that there is a synergistic effect of further promoting the hair growth effect when the maple bean leaf extract and the material are mixed with the human hair papilla cells.

 The pharmaceutical, food and cosmetic composition containing the maple leaf extract of the present invention as an active ingredient may be commercialized (medicine, food, cosmetics) in various formulations of Formulation Examples 1 to 10, but is not limited thereto. In consideration of functionality, cost and other conditions, the present invention is applicable to various formulations and formulations containing an appropriate amount of maple leaf extract. The following formulation also includes pharmaceutical, food and cosmetics containing a mixture of maple leaf extract and at least one substance selected from the group consisting of apigenin, cumestrol, caffeine, ginseng extract and green tea extract as an active ingredient. It may also be prepared in the form of a composition.

[ Formulation example  1] Preparation of Soft Capsule

Maple bean leaf extract 80mg, vitamin E 9mg, vitamin C 9mg, palm oil 2mg, vegetable hardened oil 8mg, lead 4mg and lecithin 9mg were mixed and mixed according to a conventional method to prepare a soft capsule filling solution. 400 mg per capsule was filled to prepare a soft capsule. In addition, a soft capsule sheet was prepared at a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerine, and 10 parts by weight of sorbitol solution and filled with the filler to prepare a soft capsule containing 400 mg of the composition according to the present invention.

[ Formulation example  2] Preparation of Tablet

80 mg of maple leaf extract, 9 mg of vitamin E, 9 mg of vitamin C, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose were mixed and granulated using a fluidized bed dryer, and 6 mg of sugar ester was added thereto. Tablets were prepared by compression of 504 mg of these compositions in a conventional manner.

[ Formulation example  3] Preparation of drink

Maple bean leaf extract 80mg, vitamin E 9mg, vitamin C 9mg, glucose 10g, citric acid 0.6g, and liquid oligosaccharide 25g was mixed, and then 300ml of purified water was added to each bottle to 200ml. After filling the bottle sterilized for 4 to 5 seconds at 130 ℃ to prepare a beverage.

[ Formulation example  4] Preparation of Granules

80 mg of maple leaf extract, 9 mg of vitamin E, 9 mg of vitamin C, 250 mg of anhydrous glucose, and 550 mg of starch were mixed, molded into granules by using a fluidized bed granulator, and then filled into fabrics.

[ Formulation example  5] Nutritional Cosmetics ( Milk Croissant Manufacturing

The nutrient cosmetics containing the maple leaf extract of the present invention was prepared by mixing the components of Table 3 including an oil phase component and an aqueous phase component.

Ingredient Content (% by weight) Purified water Remaining amount Maple Bean Leaf Extract 0.1 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Montana 202 (Manufacturer: Seppic) 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

[ Formulation example  6] Preparation of Massage Cream

Massage cream containing the maple leaf extract of the present invention was prepared by mixing the components of Table 4 including the oil phase component and the water phase component.

Ingredient Content (% by weight) Purified water Remaining amount Maple Bean Leaf Extract 0.1 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Montana 202 (Manufacturer: Seppic) 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

[ Formulation example  7] Manufacture of Pack

The pack containing the maple leaf extract of the present invention was prepared by mixing the components of Table 5 including the oil phase component and the water phase component.

Ingredient Content (% by weight) Purified water Remaining amount Maple Bean Leaf Extract 0.1 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Pingzi-12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, flavoring Quantity

[ Formulation example  8] Preparation of Hair Tonic

The hair tonic containing the maple leaf extract of the present invention was prepared by mixing the components of Table 6 including an oil phase component and an aqueous phase component.

Ingredient Content (% by weight) Purified water Remaining amount Maple Bean Leaf Extract 5.0 Castor Oil 5.0 Resorcinol 0.1 menthol 0.05 Panthenol 0.2 Salicylic acid 0.1 Tocopheryl acetate 0.1 Pyridoxine hydrochloride 0.1 ethanol Quantity Preservative, coloring, flavoring Quantity

[ Formulation example  9] Hair shampoo  Produce

The hair shampoo containing the maple leaf extract of the present invention was prepared by mixing the components of Table 7 including the oil phase component and the water phase component.

Ingredient Content (% by weight) Purified water Remaining amount Maple Bean Leaf Extract 5.0 Ammonium Lauryl Sulfate (ALS) 20 Ammonium Laures Sulfate (ALES) 20 Cocamide Mipa Quantity Disodium ID 0.03 Carbomer Quantity ethanol Quantity Cocamide Profile Betaine 6.0 Dimethicone (50%) 2.0 Dimethicone (70%) 2.0 DL-panthenol Quantity Tocopheryl Acetate Quantity Triethanolamine Quantity menthol Quantity Methylchloroisothiazolinone Methylisothiazolinone mixture (MC / IMI) 0.03 Methylparaben Quantity Preservative, coloring, flavoring Quantity

[ Formulation example  10] Of hair conditioner  Produce

The hair conditioner containing the maple leaf extract of the present invention was prepared by mixing the components of Table 8 including the oil phase component and the water phase component.

Ingredient Content (% by weight) Purified water Remaining amount Maple Bean Leaf Extract 5.0 ethanol 3.5 Self-emulsifying glycerin monostearate 1.5 Propylene glycol 2.5 Stearylmethylbenzyl Ammonium Chloride (25%) 7.0 Methyl paraoxybenzoate 0.3 Preservative, coloring, flavoring Quantity

The specific parts of the present invention have been described in detail above, and it is apparent to those skilled in the art that such specific descriptions are merely preferred embodiments, and thus the scope of the present invention is not limited thereto. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (14)

Hair loss prevention or hair growth composition containing maple leaf extract as an active ingredient. The composition of claim 1, wherein the maple leaf extract is extracted from the soybean leaves when some or all of the soybean leaves turn yellow. According to claim 2, wherein the maple leaf extract is characterized in that the extract from the soybean leaves of R7 to R8 step of the growth of the following beans. VE stage: 1 to 2 weeks after planting seeds, the cotyledon rises from the soil VC stage: cotyledon blooms, a word grows over it, and leaves Step V1: A word is produced from the first outer leaf, resulting in three leaves Stage V2: In the stage V1, one more word is created, resulting in three leaves Phase V3: In the V2 phase, one more word is generated, resulting in three leaves V4 stage: In the V3 stage, one more word is created, creating three leaves V5 stage: An additional word in V4 stage creates 3 leaves R2 stage: Beanweed in full bloom Step R4: Completed pod creation Step R5: Soybeans inside the pod Step R6: Green Beans Completed in the Pods R7 stage: Soybean pods and beans turning yellow R8 stage: Soybean pods and soybeans are completely yellow with leaves falling The composition of claim 1, further comprising at least one selected from apigenin, coumestrol, caffeine, ginseng extract and green tea extract. The method of claim 4, wherein When the composition contains a mixture of maple leaf extract and apigenin, the mixture contains a maple leaf extract and apigenin in a mixing ratio of 1 to 10: 1 to 10 on a ppm concentration basis, When the composition contains a mixture of maple leaf extract and cumestrol, the mixture contains a maple leaf extract and cumestrol in a mixing ratio of 1 to 10: 1 to 10, on a ppm concentration basis, When the composition contains a mixture of maple leaf extract and caffeine, the mixture contains a maple leaf extract and caffeine in a mixing ratio of 1 to 10: 1 to 10, on a ppm concentration basis, When the composition contains a mixture of Maple Bean Leaf Extract and Ginseng Extract, the mixture contains a Maple Bean Leaf Extract and Ginseng Extract in a mixing ratio of 1 to 10: 1 to 10 on a ppm concentration basis, When the composition contains a mixture of maple leaf extract and green tea extract, the mixture is characterized in that it contains maple leaf extract and green tea extract in a mixing ratio of 1 to 10: 1 to 10 on a ppm concentration basis. The composition of claim 1, wherein the content of the maple leaf extract is 1 to 60% by weight based on the total weight of the composition. The composition of claim 1, wherein the maple leaf extract promotes the activity of the Wnt / β-catenin signaling system. The composition of any one of claims 1 to 7, wherein the composition is a pharmaceutical composition, nutraceutical composition or cosmetic composition. In the manufacture of a pharmaceutical composition or medicament for preventing hair loss or hair growth, the use of maple leaf extract as a hair loss preventing agent or hair growth improving agent, The maple leaf extract is used, characterized in that extracted from the soybean leaves of R7 to R8 step of the growth of the following beans. VE stage: 1 to 2 weeks after planting seeds, the cotyledon rises from the soil VC stage: cotyledon blooms, a word grows over it, and leaves Step V1: A word is produced from the first outer leaf, resulting in three leaves Stage V2: In the stage V1, one more word is created, resulting in three leaves Phase V3: In the V2 phase, one more word is generated, resulting in three leaves V4 stage: In the V3 stage, one more word is created, creating three leaves V5 stage: An additional word in V4 stage creates 3 leaves R2 stage: Beanweed in full bloom Step R4: Completed pod creation Step R5: Soybeans inside the pod Step R6: Green Beans Completed in the Pods R7 stage: Soybean pods and beans turning yellow R8 stage: Soybean pods and soybeans are completely yellow with leaves falling The method of claim 9, wherein the pharmaceutical composition or medicament further comprises at least any one selected from apigenin (apigenin), cumestrol (Coumestrol), caffeine, ginseng extract and green tea extract , Usage. In the manufacture of a health functional food composition for preventing hair loss or hair growth, the use of maple leaf extract as a hair loss preventing agent or hair growth improving agent, The maple leaf extract is used, characterized in that extracted from the soybean leaves of R7 to R8 step of the growth of the following beans. VE stage: 1 to 2 weeks after planting seeds, the cotyledon rises from the soil VC stage: cotyledon blooms, a word grows over it, and leaves Step V1: A word is produced from the first outer leaf, resulting in three leaves Stage V2: In the stage V1, one more word is created, resulting in three leaves Phase V3: In the V2 phase, one more word is generated, resulting in three leaves V4 stage: In the V3 stage, one more word is created, creating three leaves V5 stage: An additional word in V4 stage creates 3 leaves R2 stage: Beanweed in full bloom Step R4: Completed pod creation Step R5: Soybeans inside the pod Step R6: Green Beans Completed in the Pods R7 stage: Soybean pods and beans turning yellow R8 stage: Soybean pods and soybeans are completely yellow with leaves falling According to claim 11, The dietary supplement composition is characterized in that it further contains at least one selected from apigenin (apigenin), cumestrol (Coumestrol), caffeine, ginseng extract and green tea extract, Usage. In the manufacture of a cosmetic composition for preventing hair loss or hair growth, the use of maple leaf extract as a hair loss preventing agent or hair growth improving agent, The maple leaf extract is used, characterized in that extracted from the soybean leaves of R7 to R8 step of the growth of the following beans. VE stage: 1 to 2 weeks after planting seeds, the cotyledon rises from the soil VC stage: cotyledon blooms, a word grows over it, and leaves Step V1: A word is produced from the first outer leaf, resulting in three leaves Stage V2: In the stage V1, one more word is created, resulting in three leaves Phase V3: In the V2 phase, one more word is generated, resulting in three leaves V4 stage: In the V3 stage, one more word is created, creating three leaves V5 stage: An additional word in V4 stage creates 3 leaves R2 stage: Beanweed in full bloom Step R4: Completed pod creation Step R5: Soybeans inside the pod Step R6: Green Beans Completed in the Pods R7 stage: Soybean pods and beans turning yellow R8 stage: Soybean pods and soybeans are completely yellow with leaves falling The method according to claim 13, wherein the cosmetic composition further contains at least one selected from apigenin, coumestrol, caffeine, ginseng extract, and green tea extract.

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