KR20140115400A - Composition of scalp care for prevention of depilation and improvement of hair growth comprising Rhus javanica L. Extracts - Google Patents

Abstract

The present invention relates to a scalp care composition for preventing hair loss and promoting hair growth comprising Rhus javanica L. Extracts as an active ingredient. The scalp care composition of the present invention promotes hair follicle dermal papilla cell proliferation and hair growth factor IGF-1 secretion, as well as promotes hair growth, thereby having excellent hair loss prevention and hair growth promoting effect. In addition, since the scalp care composition of the present invention is derived from a natural plant, it is harmless to the human body and has no skin side effect, so that it can be safely applied to a pharmaceutical composition for scalp care, hair loss prevention and hair growth promotion.

Description

[0001] The present invention relates to a scalp care composition for prevention of hair loss and promoting hair growth, which comprises Rhus javanica extract as an active ingredient,

The present invention relates to a scalp care composition for preventing hair loss and promoting hair growth comprising Rhus javanica L. Extracts as an active ingredient.

Hair loss is an external factor such as internal factors such as genetic cause and the action of the male hormone, androgens, or mental stress in daily life, accumulation of lipid peroxidation in the scalp, and these factors are involved in complex It is known to exhibit hair loss symptoms. Recently, it has been estimated that hair loss is not only in male hair loss but also hair loss in young people including obesity hair loss of women and it is expected to reach almost 10 million people. Therefore, socioeconomic problems are serious.

In the case of alopecia areata, hair disappears suddenly in one or several areas of the scalp. It is known that the hair follicle is attacked by lymphocytes due to immune system modulation and abruptly stops developing. In this regard, some scientists see baldness as one of the "autoimmune diseases" such as round hair loss and suggest that the male hormone (dihydrotestosterone, DHT) causes an immune response and destroys the hair follicle. In other words, in the case of people with hair loss symptoms, white blood cells in the body as an external protein to remove hair follicles, while creating a self-antibody to attack the hair follicles. When the self-antibody is produced, the thymus is atrophied and the function of the immune system is weakened. As a result, hair follicles are shrunken and the hair becomes thinner and the density is reduced, and it is escaped. In addition, the role of sebum is also emphasized as one of the main causes of hair loss. The sebaceous secretion from the hair follicles in the growing stage is induced by active oxygen or ultraviolet rays to produce lipid peroxides in the scalp layer, which hardens, and the hardened sebum blocks the pores, thereby hindering the normal growth of the hair, Necrosis is brought to promote hair loss.

5-alpha reductase (5-α-reductase) activity is reported to be higher in tissues with hair loss than tissues with normal hair. 5-alpha reductase reduces testosterone to dehydrotestosterone (DHT). In men's secondary sex, skeletal muscle growth and spermatogenesis are involved in testosterone, increasing acne, sebum, hair loss and prostate Hypertrophy and the like are known to involve dihydrotestosterone in the tissues (J. Invest. Dermatol. 1995; 105 (2): 209-14). Therefore, many studies have been conducted to find a pharmacologically active substance for treating alopecia by inhibiting the activity of 5-alpha reductase, a biosynthetic enzyme involved in the reaction of dihydrootestosterone in testosterone. Currently, there are compounds such as finasteride as a 5-alpha-reductase inhibitor, and they are used as prostate remedy and hair loss preventing agent in different doses. The finasteride is a drug which is superior in terms of convenience and efficacy, and the reagent used in manufacturing the pinasteride is expensive or toxic, which causes a burden on the manufacturing cost or pollutes the environment. Particularly, since the removal of by-products is not easy and the purity of the objective product is lowered or the reagent or the active derivative which is easily inhibited by moisture is used, there is a problem that mass production is difficult, and the steroid hormone itself is also important for the normal physiological activity , And thus, various kinds of problems may be caused when the steroid hormone production itself is artificially blocked.

Minoxidil (USA), Propecia (USA) and the like are typical drugs used for hair loss prevention and hair restoration which are currently on the market. They have excellent effect of preventing hair loss by vasodilating action and inhibitory action of male hormone It is widely used as a preparation. However, the above-mentioned medicines have some effects to prevent hair loss, but the effects related to hair growth have been reported to be somewhat poor. In other words, these preparations are effective only in the area where the hair follicles are activated, so that the effect of preventing hair loss is significant, but the growth of the hair in the resting state for a long period of time is insignificant, It was not effective at all.

In this connection, Korean Patent Laid-Open Publication No. 2010-0119226 discloses a composition for preventing hair loss or promoting hair growth comprising extracts of seaweed extracts, which is related to prevention of hair loss and promotion of hair growth. Korean Patent Publication No. 2004-0097416 Discloses a health functional food for preventing and alleviating hair loss and seborrheic dermatitis containing extract of alopoglua extract. Korean Patent Laid-Open Publication No. 2009-0021963 discloses a hair loss prevention and hair growth promoting composition comprising at least one of 2-methyl-hept-2-ene derivative and linalool oxide as an active ingredient, No. 2012-0039384 discloses a hair and hair growth composition containing a flavonoid.

Accordingly, the inventors of the present invention have found that when treating a new substance which is excellent in the effect of preventing hair loss and promoting hair growth and high in stability and having no side effects, treatment of rha bark extract with human hair cells has cell proliferation effect, And the present invention has been completed.

It is an object of the present invention to provide a scalp care composition for preventing hair loss and promoting hair growth comprising Rhus javanica Extract as an active ingredient.

It is another object of the present invention to provide a pharmaceutical composition for preventing hair loss and promoting hair growth comprising Rhus javanica Extract as an active ingredient.

The present invention provides, in one aspect, a scalp care composition for preventing hair loss and promoting hair growth, comprising a ruskia extract as an active ingredient.

In the present invention, Rhus javanica L. is a deciduous shrub belonging to Rhus verniciflua (Lepidoptera), and is native to wild animals all over the country. There is no poisoning, but there is convergence, hemostasis, detoxification, antibacterial effect of diarrhea, varicose veins, stomach ulcer, duodenal ulcer, diarrhea, wells, stool, hematuria, stomatitis etc. In addition, the roots and leaves of the rhusi were called umbel tree, and they have been used for the purpose of fever and adox, bruise, and hemostasis. The fruit of the rhusm is round, flat and covered with white and salty white powder. It is used to put the fruit of the rhusin in water, to rub the water in place of salt, or to make tofu instead of a jug. The leaf has a thick insect house, which is called a goby or a salt.

In the present invention, the rush extract can be obtained by using an extraction method and an extraction solvent known in the art, preferably water, anhydrous or lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, ), A mixed solvent of the lower alcohol and water, acetone, ethyl acetate, chloroform or 1,3-butylene glycol as an extraction solvent.

In addition, the rhusin extract can be obtained by a conventional purification process in addition to the extraction method using the extraction solvent. For example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity) The rattan extract can also be obtained through the obtained fractions.

Therefore, the present invention includes all extracts, fractions and tablets obtained in each step of extraction, fractionation or purification, and diluted solutions, concentrates or dried products thereof.

In addition, the rhusi extract of the present invention may be a bark, root, leaf, flower, stem or fruit of a rhus major, and is preferably a leaf or stem of a rhus major, but is not limited thereto.

In the present invention, the scalp care composition comprising the extract of rhodinia rhododendron as an active ingredient promotes the secretion of growth factor IGF-1 (insulin-like growth factor-1), which regulates the human hair follicle dermal papilla cell growth function and hair growth cycle It shows the efficacy of preventing hair loss and promoting hair growth.

As a result of examining the cell proliferation rate and secretion amount of IFG-1 according to the concentration of rhusi extract using human follicular dermal papilla cells, it was found that as the concentration of rhusi extract increased, the proliferation rate of follicular dermal papilla cells and IFG- 1 was increased. Therefore, it was confirmed that Rhus verniciflua extract showed the effect of promoting hair growth and preventing hair loss. In addition, it was confirmed that the hair growth promoting effect was shown by measuring the length of hair growth after treating the human hair with rattan extract.

In another concrete practice, when the hair growth inhibitor containing the rhusi extract was applied to the hair loss area of the alopecia patients for 6 months, the new hair growth began to appear from 1 month to 2 months after the treatment, And the extracts of Rhus verniciflua showed the effect of promoting hair growth.

The scalp care composition of the present invention may contain components commonly used in scalp care compositions, in addition to the extract of rush extract as an active ingredient having hair loss prevention and hair growth promoting effect. For example, adjuvants and carriers such as antidandruff agents, hair firming agents, hair restorers, scalp protectants, refreshing agents, moisturizers, softeners, antioxidants, stabilizers, vitamins and the like may be included. These adjuvants and carriers may be formulated in suitable proportions by those skilled in the art within the scope of not impairing the object of the present invention.

In addition, the scalp care composition of the present invention may be mixed with hair restorer and depilatory agent which have been conventionally used as long as they do not impair the action of hair loss prevention and hair growth promotion by Rhus javanicus extract in addition to the rusks extract.

The scalp care composition of the present invention can be used in hair cosmetics such as hair tonic, hair conditioner, hair essence, hair lotion, hair nourishing lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nourishing cream, , Hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, hair wave agent, hair bleaching agent, hair gel, hair glaze, hair dresser, hair lacquer, hair Moisturizers, hair mousses, and hair sprays, but the present invention is not limited thereto.

In the present invention, the rusks extract may be contained in an amount of 0.00001 to 15% by weight, preferably 0.0001 to 10% by weight, more preferably 0.0001 to 5% by weight based on the total weight of the composition. When the amount of the extract is less than 0.00001% by weight, the effect of preventing hair loss and promoting hair growth is weak. When the amount of the extract is more than 15% by weight, the increase of the effect of the extract is insignificant, have.

In another aspect, the present invention relates to a pharmaceutical composition for preventing hair loss and promoting hair growth, which comprises rhusin extract as an active ingredient.

The method and efficacy of the extract of rhus chinensis according to the present invention are as described above.

The pharmaceutical composition according to the present invention may contain pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the extract of rumba bark as an active ingredient having anti-hair loss and promoting hair growth. For example, excipients, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, lubricants, and flavoring agents may be included.

The pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the above-described active ingredients for administration.

The pharmaceutical form of the pharmaceutical composition may be a granule, a powder, a coated tablet, a capsule, a suppository, a liquid, a syrup, a juice, a suspension, an emulsion, a drip agent and an injectable liquid agent. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, suitable binders, lubricants, disintegrants, and coloring agents may also be included as a mixture if desired. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose and beta-lactose, corn sweeteners, acacia gum and synthetic gums and the like. Disintegrants include, but are not limited to, starch, methine cellulose, agar, bentonite, xanthan gum and the like. Acceptable pharmaceutical carriers for compositions formulated as liquid solutions include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol and mixtures of at least one of these components And other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added as needed. In addition, diluents, dispersants, surfactants, binders, and lubricants can be additionally added and formulated into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Further, it can be suitably formulated according to each disease or ingredient, using methods disclosed in Remington's Pharmaceutical Sciences (19th ed., 1995), as appropriate in the field.

The pharmaceutical composition of the present invention can be administered orally or parenterally, and is preferably applied by parenteral administration, more preferably topical application by application.

The pharmaceutical composition may be suitably used as an external preparation for skin intended for topical administration, which is applied directly to the affected area. In this case, the cream, gel, patch, spray, ointment, warning agent, lotion, liniment, Cataplasma, and the like, but the present invention is not limited thereto.

The pharmaceutical composition is administered in a pharmaceutically effective amount. In the present invention, a pharmaceutically effective amount means an amount sufficient to treat or prevent a disease at a reasonable benefit / risk ratio applicable to medical treatment or prevention. The effective dose level will depend on the type of disease and its severity, Including the age, weight, health and sex of the patient, sensitivity of the patient to the drug, time of administration of the particular extract used, route of administration and rate of release, duration of treatment, Can be determined according to factors well known in the art. Generally, it is 1.0 ml to 3.0 ml on an adult basis, and it is preferable to apply once to 5 times a day, if necessary, and continue for 1 month or longer.

The Rhus verniciflua extract may be contained in an amount of 0.00001 wt% to 15 wt%, preferably 0.0001 wt% to 10 wt%, and more preferably 0.0001 wt% to 5 wt%, based on the total weight of the pharmaceutical composition. When the amount of the extract is less than 0.00001% by weight, the effect of preventing hair loss and promoting hair growth is weak. When the amount of the extract is more than 15% by weight, the increase of the effect of the extract is insignificant, have.

The scalp care composition of the present invention comprising the extract of rush extract as an active ingredient has the effect of preventing hair loss and promoting hair growth. And can be safely applied to a pharmaceutical composition for preventing scalp care and hair loss and promoting hair growth without skin side effects.

FIG. 1 shows the proliferation rate of human hair follicular dermal papilla cells according to the concentration of the rhusi extract of the present invention.
FIG. 2 shows the ratio of IGF-1 in hair growth factor according to the concentration of rhusi extract of the present invention.
Fig. 3 shows the hair growth rate by treatment with rattan extract of the present invention.

Hereinafter, the present invention will be described in more detail with reference to examples. It is to be understood that these embodiments are provided by way of illustration only, and are not intended to limit the scope of the invention.

Example 1: Preparation of rattan extract

1kg of leaf and stem of rhusmia (Jeju Island, 2012) was washed, dried, pulverized and powdered, and then 10L of 70% ethanol was added and extracted with stirring for 24 hours. The extract solution was filtered through a filter paper (Whatman, Advantes, No. 2), concentrated under reduced pressure, and lyophilized to prepare a rattan extract.

Example  2: In - vitro  Prevention of hair loss and promotion of hair growth in rattan extract

2-1. Identification of human hair follicle dermis papillary cell proliferation rate of rattan extract

Human hair follicle dermal papillary cell (Application, Inc. USA) was inoculated into a 6-well microplate containing DMEM medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) ^Five cells were inoculated and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours. The next day, the cells were washed once with the serum-free culture medium, and then replaced with the culture medium in which the serum was removed. Then, rattan extracts were treated at 1, 10, and 50 ppm and cultured for 72 hours. Then, the cells were washed once with the serum-free culture medium, replaced with serum-free medium containing 10% MTT, reacted for 3 hours, and the absorbance (OD) value was measured and converted into a percentage. The results are shown in Fig.

The results showed that the number of human hair follicle dermal papilla cells increased as the concentration of rusks extract increased.

2-2. Measurement of IGF-1 secretion promoting effect of Rhus javanicus extract

The human hair dermal papilla cells were inoculated into a 24-well microplate at 7.5 × 10 ⁴ cells and cultured at 37 ° C. for 24 hours in a 5% CO ₂ incubator. The culture medium was replaced with DMEM without antibiotics and incubated for 16 hours. Each well was treated with 1, 10, and 50 ppm of rusk extract and cultured again for 48 hours, and then only the cell culture was collected.

Secretion of insulin-like growth factor-1 (IGF-1) was measured using the growth factor IGF-1 assay kit (DG100, R & D systems, USA). The results are shown in Fig.

As a result, IGF-1 production was increased in human dermal papilla cells as the concentration of rhusi extract increased.

Example 3: Ex-vivo  Prevention of hair loss and promotion of hair growth in rattan extract

The hair is separated from the human scalp and transferred to a 48-well microplate and cultured in Philpott's medium at 37 ° C and 5% CO ₂ . The Philpott medium was supplemented with 10 μg / ml insulin (Sigma, USA), 10 ng / ml hydrocortisone (Sigma, USA), 10 μg / ml transferrin (Sigma, USA) ml sodium ascleic acid (Sigma, USA) and 50 U / ml gentamicin (Welgene, Korea). Then, the wells were treated with 50 ppm rattan extract and cultured for 7 days while changing the culture every 2 days.

The length of hair growth was calculated by subtracting hair length from the hair length measured on the last day of culturing by subtracting hair length from the length of hair on day 1 after treatment with rattan extract. The results are shown in Fig.

As a result, the hair growth of the hair tissue treated with rhusin extract showed better hair growth promotion than that of the rattan extract treated group.

Example 4: In-vivo  Prevention of hair loss and promotion of hair growth in rattan extract

4-1. Production of a hair growth agent containing Rhus verniciflua extract

Table 1 < tb >< tb >< tb >< tb >< tb > Specifically, the distilled water as the water phase was heated to 70 占 폚, and the oil-based preservative and thickeners were heated to 70 占 폚 and stirred with a homomixer (Tokushu Kika, Japan) to emulsify. After the emulsification was completed, the solution was cooled to 45 캜, and the rattan extract was added and dispersed in an amount of 0.1 and 1.0% by weight based on the total weight of the composition, followed by cooling to 30 캜. Test groups 1 and 2 are each a hair growth promoter containing Rhus verniciflua extract.

content(%) ingredient Test group 1 Test group 2 Rattan extract 0.1 1.0 Preservative (Gramben) 0.5 0.5 Xanthan-Gum < / RTI > 0.5 0.5 Distilled water 98.9 98 Gross weight 100 100

4-2. Prevention of Hair Loss and Promotion of Hair Growing Effect of Hair Growing Agent Containing Rhus javanica Extract

Forty patients, ranging from 40s to late 60s, were divided into four groups, each with 10 follicles per group, including hairy scalp hairy scalp hair follicles, typical male alopecia, and acute alopecia areata. The barnyardgrass containing Rhodiola extract prepared in 5-1 above was applied to hair loss sites of alopecia of each group twice a day, 3cc each for 6 months. Hair loss and hair growth were observed on a monthly basis. As a comparative group, commercially available Moxidil (Hanmi Pharm) was used, and only 50% ethanol was used as a control group. The results are shown in Table 2.

The criteria are as follows:

4: High Effective = Newborn Eye

3: moderate effect = newborn eye (fluffy)

2: slightly effective = reduced number of hair loss

1: No effect = No change at all

Control group Comparative group Test group 1 Test group 2 Efficacy / Effect 2.6 4.5 3.2 3.7

As shown in the above Table 2, in the alopecia treated with the hair growth promoter containing the rhusi extract, new hair of the bristles such as the fluffy hair began to appear from 1 month or 2 months after the treatment, and at 6 months after the treatment, Effect was observed. In addition, it was confirmed that the newborn moth was continuously grown and the hair loss phenomenon was not found.

Example 5: Safety test for human skin of rattan extract

5-1. Production of external preparations for skin including rhusi extract

In order to confirm that the extracts of Rhus wood extracts were safe for human skin, skin external preparations containing Rhus verniciflua extracts were prepared by the ingredients and contents shown in Table 3 below. First, purified water, glycerin, and butylene glycol were mixed and dissolved at a temperature of about 70 DEG C (water phase part), and the other components were dissolved at about 70 DEG C (oil phase part) except for the three components and trimethanolamine. Thereafter, the oil part was added to the water-based part, and the mixture was firstly emulsified with a homomixer (Tokushu Kika, Japan), followed by final addition of trimethanolamine. Next, the bubbles generated in the mixed solution were removed, and the mixture was cooled to room temperature to prepare an external preparation for skin.

ingredient content (%) Test group 1 Test group 2 Test group 3 Control group Purified water 71 71 71 71 glycerin 8.0 8.0 8.0 8.0 Butylene glycol 4.0 4.0 4.0 4.0 Rattan extract One 2 5 0 Caprylic Capri Triglyceride 8.0 8.0 8.0 8.0 Squalane 5.0 5.0 5.0 5.0 Cetearyl glucide 1.5 1.5 1.5 1.5 Sorbitan stearate 0.4 0.4 0.4 0.4 Cetearyl alcohol 1.0 1.0 1.0 1.0 Trimethanolamine 0.1 0.1 0.1 0.1 Gross weight 100 100 100 100

5-2. Experiment of cumulative stimulation of skin

The skin external stimulants prepared by the method of 6-1 were applied to 30 healthy adults for 9 times and 24 hours cumulative pelletization on the upper forearm. As a control, skin external preparation without squalene extract was used.

The patching method was carried out using a Finn chamber (Epitest Ltd., Finland), and 15 占 퐇 of each skin external agent was dripped into the chamber, followed by applying a patch. The degree of the skin reaction on each occasion was scored using Equation 1 below, and the results are shown in Table 4 below.

[Experimental Equation 1]

(Number of total subjects × maximum score (4 points))}] × 100] / number of inspections (9 times)

At this time, ±, 1, 2, and ++ are given as ±, 1, and 4, respectively, and the composition is judged to be safe when the average reactivity is less than 3.

Test substance Number of subjects who responded (persons) Average
Reactivity 1 week 2 weeks 3 weeks Primary Secondary Third Fourth 5th 6th Seventh 8th car 9th ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ Control group 1/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0.09 Test group 1 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0.00 Test group 2 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0.00 Test group 3 1/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0.09 Subject 30 30 30 30 30 30 30 30 30

As shown in Table 3, the number of persons corresponding to ±, +, and ++ in the control group was 1, 0, and 0 in the primary pellet, respectively, and no skin reaction occurred after the secondary pellet. In the test groups 1 and 2 treated with 1 wt.% And 2 wt.% Of rhus chinensis extract, no skin reaction was observed in all the test groups tested. In the case of test group 3 containing 5 wt% of rhusi extract, the number of persons corresponding to ±, +, and ++ was 1, 0, and 0 in the primary pellet, respectively, and the skin reacted after the secondary pellet I did. As a result of calculation according to Equation 1, the average reactivity of the control group and the test group 3 was 0.09, and the average reactivity of the test groups 1 and 2 was 0.00, which was less than 3.

Claims (6)

A scalp care composition for preventing hair loss and promoting hair growth, comprising the extract of Rhodiola as an active ingredient.
The cosmetic composition according to claim 1, wherein the cosmetic composition is selected from the group consisting of hair tonic, hair conditioner, hair essence, hair lotion, hair nourishing lotion, hair shampoo, hair conditioner, hair treatment, Hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, hair wave agent, hair bleaching agent, hair gel, hair glaze, hair dresser, hair Lacquers, hair moisturizers, hair mousses, and hair sprays. ≪ RTI ID = 0.0 > 11. < / RTI >
The scalp care composition according to claim 1, wherein the rhusin extract is contained in an amount of 0.00001 to 15% by weight based on the total weight of the composition.
A pharmaceutical composition for prevention of hair loss and promoting hair growth, comprising as an active ingredient an extract of Rhus javanica.
The pharmaceutical composition according to claim 4, wherein the pharmaceutical composition is at least one selected from the group consisting of creams, gels, patches, sprays, ointments, alerts, lotions, liniments, pastes and cataplasms ≪ / RTI >
5. The pharmaceutical composition according to claim 4, wherein the rumen extract is present in an amount of 0.00001 to 15% by weight based on the total weight of the composition.

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