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WO2017014828A3 - Dispositif et procédé de détection électrochimique - Google Patents

Dispositif et procédé de détection électrochimique Download PDF

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Publication number
WO2017014828A3
WO2017014828A3 PCT/US2016/032706 US2016032706W WO2017014828A3 WO 2017014828 A3 WO2017014828 A3 WO 2017014828A3 US 2016032706 W US2016032706 W US 2016032706W WO 2017014828 A3 WO2017014828 A3 WO 2017014828A3
Authority
WO
WIPO (PCT)
Prior art keywords
zone
binding
test zone
sample deposition
embedded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2016/032706
Other languages
English (en)
Other versions
WO2017014828A9 (fr
WO2017014828A2 (fr
Inventor
Maria-Nefeli TSALOGLOU
Alex Nemiroski
Dionysios CHRISTODULEAS
Gulden CAMCI-UNAL
John T. CONNELLY
Maria Teresa FERNANDEZ-ABEDUL
George M. Whitesides
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Southampton
Harvard University
Original Assignee
University of Southampton
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Southampton, Harvard University filed Critical University of Southampton
Publication of WO2017014828A2 publication Critical patent/WO2017014828A2/fr
Publication of WO2017014828A9 publication Critical patent/WO2017014828A9/fr
Publication of WO2017014828A3 publication Critical patent/WO2017014828A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un dispositif microfluidique et électrochimique permettant de détecter un matériel génétique. Ledit dispositif comprend : une ou plusieurs couches cellulosiques comprenant une zone de test hydrophile et/ou une zone de dépôt d'échantillon hydrophile en communication fluidique l'une avec l'autre; un ou plusieurs agents d'amplification sélectionnés pour amplifier un matériel génétique et intégrés dans la zone de test hydrophile ou dans la zone de dépôt d'échantillon; un ou plusieurs agents liants intégrés dans la zone de test ou dans la zone de dépôt d'échantillon et sélectionnés pour lier le matériel génétique amplifié pour entraîner un changement de la concentration d'un produit chimique de signalisation, le produit chimique de signalisation est soit intégré dans la zone de test ou dans la zone de dépôt d'échantillon avant la liaison, soit est un produit nouvellement généré de la liaison; et un ensemble d'électrodes comprenant une ou plusieurs électrodes en contact fluidique avec la zone de test et configurées pour interagir avec le produit chimique de signalisation pour entraîner un changement courant lisible par un lecteur électrochimique. La présente invention concerne en outre des procédés d'utilisation du dispositif et un kit contenant le dispositif.
PCT/US2016/032706 2015-05-20 2016-05-16 Dispositif et procédé de détection électrochimique Ceased WO2017014828A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562164079P 2015-05-20 2015-05-20
US62/164,079 2015-05-20

Publications (3)

Publication Number Publication Date
WO2017014828A2 WO2017014828A2 (fr) 2017-01-26
WO2017014828A9 WO2017014828A9 (fr) 2017-02-16
WO2017014828A3 true WO2017014828A3 (fr) 2017-03-16

Family

ID=57834491

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2016/032706 Ceased WO2017014828A2 (fr) 2015-05-20 2016-05-16 Dispositif et procédé de détection électrochimique

Country Status (1)

Country Link
WO (1) WO2017014828A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017184665A1 (fr) * 2016-04-19 2017-10-26 Purdue Research Foundation Vannes régulées en température pour systèmes microfluidiques à base de papier
CA3175922A1 (fr) * 2020-03-17 2021-09-23 Detect, Inc. Test de diagnostic rapide
BE1028157B9 (fr) * 2020-04-07 2022-07-11 Vaccim Dispositif et procédé pour déterminer, au départ d'un échantillon préalablement prélevé chez un individu, qu'au moins un anticorps cible a été ou est présent chez ledit individu
WO2021204860A1 (fr) * 2020-04-07 2021-10-14 Trivarop Srl Dispositif et procédé pour déterminer, au départ d'un échantillon préalablement prélevé chez un individu, qu'au moins un anticorps cible a été ou est présent chez ledit individu
CN111781351B (zh) * 2020-07-15 2023-03-28 桂林电子科技大学 用于新型冠状病毒抗原定量检测的电化学免疫层析试纸条及其制备方法
CN112098479B (zh) * 2020-09-17 2022-09-02 中国科学院空天信息创新研究院 微型化智能便携式直接免疫的电化学检测装置及检测方法
BE1029278B1 (fr) * 2021-04-07 2022-11-17 Magnetrap Dispositif pour détecter la présence d'au moins un ADN cible et/ou d'au moins un ARN cible dans un échantillon, en particulier dans un échantillon liquide

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120181184A1 (en) * 2009-03-06 2012-07-19 President And Fellows Of Harvard College Microfluidic, electrochemical devices
US20140042038A1 (en) * 2008-04-14 2014-02-13 University Of South Florida Microfluidic electrochemical genotyping system
US20140183059A1 (en) * 2011-09-06 2014-07-03 President And Fellows Of Harvard College Microfluidic devices for multiplexed electrochemical detection
WO2014152825A1 (fr) * 2013-03-14 2014-09-25 Diagnostics For All, Inc. Dispositifs de diagnostic moléculaire à composants magnétiques

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140042038A1 (en) * 2008-04-14 2014-02-13 University Of South Florida Microfluidic electrochemical genotyping system
US20120181184A1 (en) * 2009-03-06 2012-07-19 President And Fellows Of Harvard College Microfluidic, electrochemical devices
US20140183059A1 (en) * 2011-09-06 2014-07-03 President And Fellows Of Harvard College Microfluidic devices for multiplexed electrochemical detection
WO2014152825A1 (fr) * 2013-03-14 2014-09-25 Diagnostics For All, Inc. Dispositifs de diagnostic moléculaire à composants magnétiques

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MAXWELL ET AL.: "Paper-based electroanalytical devices for accessible diagnostic testing", MRS BULLETIN, vol. 38, 12 April 2013 (2013-04-12), pages 309 - 314, XP055369444 *
NEMIROSKI ET AL.: "Universal mobile electrochemical detector designed for use in resource-limited applications", PROC NATL ACAD SCI USA, vol. 111, 4 August 2014 (2014-08-04), pages 11984 - 11989, XP055211323 *

Also Published As

Publication number Publication date
WO2017014828A9 (fr) 2017-02-16
WO2017014828A2 (fr) 2017-01-26

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