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WO2017014828A3 - Device and method for electrochemical detection - Google Patents

Device and method for electrochemical detection Download PDF

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Publication number
WO2017014828A3
WO2017014828A3 PCT/US2016/032706 US2016032706W WO2017014828A3 WO 2017014828 A3 WO2017014828 A3 WO 2017014828A3 US 2016032706 W US2016032706 W US 2016032706W WO 2017014828 A3 WO2017014828 A3 WO 2017014828A3
Authority
WO
WIPO (PCT)
Prior art keywords
zone
binding
test zone
sample deposition
embedded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2016/032706
Other languages
French (fr)
Other versions
WO2017014828A9 (en
WO2017014828A2 (en
Inventor
Maria-Nefeli TSALOGLOU
Alex Nemiroski
Dionysios CHRISTODULEAS
Gulden CAMCI-UNAL
John T. CONNELLY
Maria Teresa FERNANDEZ-ABEDUL
George M. Whitesides
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Southampton
Harvard University
Original Assignee
University of Southampton
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Southampton, Harvard University filed Critical University of Southampton
Publication of WO2017014828A2 publication Critical patent/WO2017014828A2/en
Publication of WO2017014828A9 publication Critical patent/WO2017014828A9/en
Publication of WO2017014828A3 publication Critical patent/WO2017014828A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Dispersion Chemistry (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A microfluidic, electrochemical device for detecting a genetic material is described, including: one or more cellulosic layers comprising at least one of a hydrophilic test zone and a hydrophilic sample deposition zone in fluid communication with each other; one or more amplification agents selected for amplifying a genetic material and embedded in the hydrophilic test zone or the sample deposition zone; one or more binding agents embedded in the test zone or the sample deposition zone and selected for binding the amplified genetic material to result in a change of the concentration of a signaling chemical, wherein the signaling chemical is either embedded in the test zone or the sample deposition zone prior to the binding or is a newly generated product of the binding; and an electrode assembly comprising one or more electrodes in fluidic contact with the test zone and configured to interact with the signaling chemical to result in a current change readable by an electrochemical reader.
PCT/US2016/032706 2015-05-20 2016-05-16 Device and method for electrochemical detection Ceased WO2017014828A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562164079P 2015-05-20 2015-05-20
US62/164,079 2015-05-20

Publications (3)

Publication Number Publication Date
WO2017014828A2 WO2017014828A2 (en) 2017-01-26
WO2017014828A9 WO2017014828A9 (en) 2017-02-16
WO2017014828A3 true WO2017014828A3 (en) 2017-03-16

Family

ID=57834491

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2016/032706 Ceased WO2017014828A2 (en) 2015-05-20 2016-05-16 Device and method for electrochemical detection

Country Status (1)

Country Link
WO (1) WO2017014828A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017184665A1 (en) * 2016-04-19 2017-10-26 Purdue Research Foundation Temperature controlled valves for paper-based microfluidic systems
CA3175922A1 (en) * 2020-03-17 2021-09-23 Detect, Inc. Rapid diagnostic test
BE1028157B9 (en) * 2020-04-07 2022-07-11 Vaccim Device and method for determining, from a sample previously taken from an individual, that at least one target antibody has been or is present in said individual
WO2021204860A1 (en) * 2020-04-07 2021-10-14 Trivarop Srl Device and method for determining, from a sample previously collected from an individual, that at least one target antibody has been or is present in said individual
CN111781351B (en) * 2020-07-15 2023-03-28 桂林电子科技大学 Electrochemical immunochromatographic test strip for quantitative detection of novel coronavirus antigen and preparation method thereof
CN112098479B (en) * 2020-09-17 2022-09-02 中国科学院空天信息创新研究院 Miniaturized intelligent portable direct immunization electrochemical detection device and detection method
BE1029278B1 (en) * 2021-04-07 2022-11-17 Magnetrap Device for detecting the presence of at least one target DNA and/or at least one target RNA in a sample, in particular in a liquid sample

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120181184A1 (en) * 2009-03-06 2012-07-19 President And Fellows Of Harvard College Microfluidic, electrochemical devices
US20140042038A1 (en) * 2008-04-14 2014-02-13 University Of South Florida Microfluidic electrochemical genotyping system
US20140183059A1 (en) * 2011-09-06 2014-07-03 President And Fellows Of Harvard College Microfluidic devices for multiplexed electrochemical detection
WO2014152825A1 (en) * 2013-03-14 2014-09-25 Diagnostics For All, Inc. Molecular diagnostic devices with magnetic components

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140042038A1 (en) * 2008-04-14 2014-02-13 University Of South Florida Microfluidic electrochemical genotyping system
US20120181184A1 (en) * 2009-03-06 2012-07-19 President And Fellows Of Harvard College Microfluidic, electrochemical devices
US20140183059A1 (en) * 2011-09-06 2014-07-03 President And Fellows Of Harvard College Microfluidic devices for multiplexed electrochemical detection
WO2014152825A1 (en) * 2013-03-14 2014-09-25 Diagnostics For All, Inc. Molecular diagnostic devices with magnetic components

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MAXWELL ET AL.: "Paper-based electroanalytical devices for accessible diagnostic testing", MRS BULLETIN, vol. 38, 12 April 2013 (2013-04-12), pages 309 - 314, XP055369444 *
NEMIROSKI ET AL.: "Universal mobile electrochemical detector designed for use in resource-limited applications", PROC NATL ACAD SCI USA, vol. 111, 4 August 2014 (2014-08-04), pages 11984 - 11989, XP055211323 *

Also Published As

Publication number Publication date
WO2017014828A9 (en) 2017-02-16
WO2017014828A2 (en) 2017-01-26

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