WO2016107107A1 - Animal-derived nerve scaffold and manufacturing method therefor - Google Patents

Abstract

An animal-derived nerve scaffold and a manufacturing method therefor. The animal-derived nerve scaffold comprises a scaffold membrane (1) and a degradable metal wire (2) wrapped by the scaffold membrane (1). The primary constituents of the scaffold membrane (1) are animal-derived blood vessels, lymphatic vessels, neural tubes or intestines. The manufacturing method comprises the steps of preparing an animal-derived membrane powder and wrapping the degradable metal wire (2) with the scaffold membrane (1).

Description

Animal source nerve scaffold and preparation method thereof Technical field

The invention relates to the technical field of nerve repair, and in particular to an animal-derived nerve stent and a preparation method thereof.

Background technique

Peripheral nerve damage or breakage due to various trauma causes a decrease or loss of sensory and motor function. Severe peripheral nerve injury often leads to paralysis or permanent loss of labor. Peripheral nerve injury has a high incidence in China. According to statistics, in trauma patients, nerve injury of the extremities accounts for about 10% of the total number of trauma, and about 60% of the fractures of firearm injuries have neurological injuries. To this end, we need to find the ideal treatment for peripheral nerve injury. For peripheral nerve fractures, if the fracture gap is large, the fractured peripheral nerve must be regenerated and repaired by bridging the graft.

At present, commonly used implants include artificial nerve stents and autografts. In terms of artificial nerve stents, the development of non-degradable catheter materials may lead to nerve compression and the risk of secondary surgery. In recent years, research has mainly led to the development and research of biodegradable stent materials. Currently, the United States has approved a variety of brands, such as NeuraGen, SaluBridge, Neurolac and other nerve catheters for clinical use. However, China's research on nerve scaffolds is relatively backward, and no neural catheters with independent intellectual property rights have been developed.

Autologous transplantation is undoubtedly the most ideal method for repairing nerve defects. However, a large number of experimental and clinical results have confirmed that autologous nerve transplantation is superior to artificial stents. However, it is well known that autologous nerve transplantation inevitably causes new nerve damage, resulting in impaired function of the donor site, and even local formation of scars, neuroma, resulting in motor or sensory disturbances. Moreover, autologous nerve sources are limited, especially for long segments of important large nerves, and it is often difficult to find a suitable donor. Therefore, the development of autologous nerve transplantation is very limited and difficult to promote. Therefore, it is especially necessary to find nerve defect repair materials and methods to replace autologous nerve transplantation.

Summary of the invention

In order to overcome the deficiencies of the prior art, the present invention provides an animal-derived nerve stent and a preparation thereof The method, the nerve scaffold is of animal origin, solves the problem of insufficient supply of autologous nerves, and also solves the problem that autologous nerve transplantation inevitably causes new nerve damage, leading to impaired function of the donor area, and has far-reaching significance for nerve repair.

The technical solution of the present invention is as follows: an animal-derived nerve stent comprising a stent film and a degradable metal wire wrapped by a stent film, the main component of which is an animal-derived blood vessel, a lymphatic vessel, a neural tube or an intestinal tract. .

The stent is in the form of a sheet, a strip or a cylinder.

The main component of the degradable metal wire is magnesium, zinc, calcium, iron or an alloy thereof.

The degradable wires are distributed along the longitudinal and transverse directions of the nerve stent.

The method for preparing the animal-derived nerve stent comprises the following steps:

1) Animal source film powder: animal source blood vessels, lymphatic vessels, neural tubes or intestines are freeze-dried, sterilized and pulverized into powder, and animal source film powder is obtained;

2) Preparation of the stent: the animal source film powder is completely dissolved in methylene chloride, and the ultrasonic dispersion is uniform, and the slurry is obtained, which is transferred into the mold cavity of the film forming mold, and the horizontal and vertical directions are placed in the mold cavity along the stent forming direction. The metal wire is degraded, the degradable metal wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.

Raw materials such as blood vessels, lymphatic vessels, neural tubes or intestines have good biocompatibility with human body and can provide morphological support for nerve growth. Scanning electron fiber mirrors show that the internal structure of the obtained animal-derived nerve scaffold is Honeycomb provides space for the nerve cells to crawl and grow; the degradable metals and their alloys can guide the growth of nerve cells, thereby facilitating the repair of nerve cells.

The invention has the beneficial effects that the nerve scaffold of the invention is of animal origin, solves the problem of insufficient supply of autologous nerve, and solves the problem that the autologous nerve transplantation inevitably causes new nerve damage, resulting in impaired function of the donor area, Nerve repair has far-reaching significance.

BRIEF DESCRIPTION OF THE DRAWINGS:

1 is a schematic view showing the structure of a collagen-rich artificial nerve stent of the present invention.

detailed description

The present invention will be further described below in conjunction with the drawings and preferred embodiments, but the embodiments of the present invention are not limited thereto.

Referring to Fig. 1, an animal-derived nerve stent comprises a stent film 1 and a degradable metal wire 2 wrapped by a stent film 1. The degradable metal wire 2 is distributed along the longitudinal and transverse directions of the nerve stent.

The stent can be prepared into various shapes according to the needs of use, and is generally cylindrical or sheet-shaped.

The main component of the scaffold film 1 is an animal-derived blood vessel, a lymphatic vessel, a neural tube or an intestinal tract. The main component of the degradable metal wire 2 is magnesium, zinc, calcium, iron or an alloy thereof.

Example 1: A method for preparing the animal-derived nerve stent, comprising the following steps:

1) Animal source film powder: animal source blood vessels are freeze-dried, sterilized and pulverized into powder, and animal source film powder is obtained;

2) Preparation of the stent: the animal source film powder is completely dissolved in methylene chloride, the ultrasonic dispersion is uniform, the slurry is obtained, and transferred into the mold cavity of the film forming mold, and the transverse and longitudinal magnesium are placed in the mold cavity along the stent forming direction. Zinc alloy wire, the magnesium-zinc alloy wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.

Example 2: A method for preparing the animal-derived nerve stent, comprising the following steps:

1) Animal source film powder: the animal source neural tube is freeze-dried, sterilized and pulverized into powder, and the animal source film powder is obtained;

2) Preparation of the stent: the animal source film powder is completely dissolved in methylene chloride, and the ultrasonic dispersion is uniform, and the slurry is obtained, which is transferred into the mold cavity of the film forming mold, and the horizontal and vertical directions are placed in the mold cavity along the stent forming direction. The metal wire is degraded, the degradable metal wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.

Example 3: A method for preparing the animal-derived nerve stent, comprising the following steps:

1) Animal source film powder: the animal source is lyophilized and dried, sterilized and pulverized into powder, and the animal source film powder is obtained;

2) Preparation of the stent: the animal source film powder is completely dissolved in methylene chloride, and the ultrasonic dispersion is uniform, and the slurry is obtained, which is transferred into the mold cavity of the film forming mold, and the horizontal and vertical directions are placed in the mold cavity along the stent forming direction. The metal wire is degraded, the degradable metal wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.

The above is a further detailed description of the present invention in connection with the specific preferred embodiments, and the specific embodiments of the present invention are not limited to the description. For those skilled in the art to which the present invention pertains, the architectural form can be flexible and can be derived from a series of products without departing from the inventive concept. It is to be understood that the invention is intended to be limited to the scope of the invention as defined by the appended claims.

Claims (5)

An animal-derived nerve stent is characterized in that it comprises a stent film and a degradable metal wire wrapped by a stent film, and the main component of the stent film is an animal-derived blood vessel, a lymphatic vessel, a neural tube or an intestinal tract. The animal-derived nerve stent according to claim 1, wherein the stent is in the form of a sheet, an elongated strip or a cylinder. The animal-derived nerve stent according to claim 1, wherein the main component of the degradable metal wire is magnesium, zinc, calcium, iron or an alloy thereof. The animal-derived nerve stent of claim 1 wherein said degradable metal wire is distributed longitudinally and laterally of said nerve stent. The method for preparing an animal-derived nerve stent according to any one of claims 1 to 4, comprising the steps of: 1) Animal source film powder: animal source blood vessels, lymphatic vessels, neural tubes or intestines are freeze-dried, sterilized and pulverized into powder, and animal source film powder is obtained; 2) Preparation of the stent: the animal source film powder is completely dissolved in methylene chloride, and the ultrasonic dispersion is uniform, and the slurry is obtained, which is transferred into the mold cavity of the film forming mold, and the horizontal and vertical directions are placed in the mold cavity along the stent forming direction. The metal wire is degraded, the degradable metal wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.

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