WO2016195126A1 - Blood separation and extraction apparatus for extracting platelet-rich growth factor - Google Patents

Abstract

The present invention carries out centrifugation in a state in which blood is accommodated in an accommodation space part formed at the lower part of a main tube, injects white blood cells, which are a separation layer of the centrifuged blood, into a separation space part of the main tube, and then couples an upper cap such that the upper cap communicates with a passage part of the main tube, thereby allowing only concentrated platelets to be collected and extracted, and thus the separation of blood and the extraction operation of concentrated platelets can simultaneously proceed by carrying out centrifugation once and with one container.

Description

Blood separation and extraction device for platelet-rich growth factor extraction

The present invention relates to a blood separation and extraction device for platelet-rich growth factor extraction, in particular to allow the separation and concentration operation to proceed in one container at the same time, and to perform the separation and extraction by only the first centrifugation The present invention relates to a blood separation and extraction apparatus for platelet-rich growth factor extraction with improved structure.

The present invention also relates to a blood separation and extraction apparatus for platelet-rich growth factor extraction whose structure has been improved so that most of the leukocytes extracted in the container can be recovered using a syringe.

Blood circulating in blood vessels in a human or animal body carries oxygen taken from the lungs to tissue cells, transports carbon dioxide from the tissues to the lungs for release, and transports nutrients absorbed from the digestive tract to organs or tissue cells, As a decomposition product of, it transports substances that are unnecessary to the living body to the kidneys and discharges them out of the body, carries hormones secreted from the endocrine glands to the working organs and tissues, distributes body heat evenly, maintains constant body temperature, and invades the living body It performs various functions such as destroying and detoxifying bacteria and foreign substances.

Although such blood is used as a major indicator for determining various diseases or health conditions, platelets and leukocytes rich in growth factors (Growth factor) in the blood are used for therapeutic purposes.

Blood is composed of red blood cells, white blood cells, platelets, etc. Among them, platelets are mainly present in plasma, and plasma is divided into a platelet rich plasma (PRP) layer and a plate poor plasma (PPP) layer.

Platelet-rich plasma, called Platelet Rich Plasma (PRP), is located at the bottom of the plasma and contains growth factors such as cytokines, PDGF, TGF-BETA1, and VEGP. A good effect on healing is found in the literature.

Platelet-free plasma, also called PPP (Platelet Poor Plasma), is used as an autologous filler or a cosmetic product derived from autologous plasma.

PRP layer is only about 1% of the collected blood and the viscosity is high, it is difficult to collect because it is attached to red blood cells. PRP is implanted in the pain area, especially the knee restraint, ligaments and muscles, and it is used for therapeutic purposes because it stimulates the stem cells around and helps to generate the cells. Sampling was difficult, and red blood cells entered the human body, causing considerable pain, which could cause inflammation, and the emphasis was on the technique of collecting concentrated platelets (PRP), except red blood cells.

However, in order to extract the existing thick platelet layer, the first and second centrifugation processes have a process of concentrating, and this process is performed after injecting the blood into the lower liquid chamber of the primary separation vessel having the upper and lower liquid chambers. The injected primary separation vessel is first centrifuged, and the leukocytes and buffy coat layer separated in the first centrifugation process are pushed to the upper liquid chamber.

Thereafter, the leukocytes and buffy coat layer of the upper liquid chamber are transferred to the secondary separation vessel side, and further centrifuged and concentrated to extract concentrated platelets.

At this time, not only the loss occurs in the process of transferring the separation layer of the first centrifuged blood to the secondary separation vessel side, there is a problem that the first and second centrifugal separation work is cumbersome and takes a long time.

The present invention was created in view of the above-mentioned problems, and the object thereof is to allow a process for extracting and extracting concentrated platelets into a single container and simplifying the centrifugal separation process. The present invention provides a blood separation and extraction device for platelet-rich growth factor extraction, the structure of which is improved to enable the separation and extraction of concentrated platelets only by centrifugation.

Another object of the present invention is to provide a blood separation and extraction apparatus for platelet-rich growth factor extraction, the structure of which is improved so that the separation and extraction of platelets in the separation layer of the separated blood easily.

It is another object of the present invention to provide a blood separation and extraction apparatus for extracting platelet-rich growth factors whose structure is improved so that most of the leukocytes extracted in the container can be recovered using a syringe.

The present invention for achieving the above object is formed in the lower accommodating space for accommodating blood, the inclined portion has a narrower inner diameter than the lower portion formed in the upper portion of the accommodating space is formed in the upper passage is formed, the centrifugation of the blood A body tube having a separation space portion in which a portion of the separation layer of blood separated by separation is received via the passage portion;

A slider which is slidably coupled to the accommodation space and pushes the separation layer of blood separated by the centrifugation to the separation space through the passage;

Removably coupled to the upper end of the body tube, the coupling portion is formed to be coupled to the upper end of the passage portion when coupled with the body tube in the lower center, is formed to extend to the lower side of the coupling portion of the blood when the slider is raised And an upper cap having a storage unit in which concentrated platelets are introduced and accommodated in a separation layer of the separation layer.

The upper cap is an injection port through which the first syringe for injecting the blood through the passage portion on the upper surface thereof, a first outlet through which the second syringe extracts the concentrated platelets contained in the reservoir to the outside; A second outlet for allowing entry of the third syringe for extracting the white blood cells contained in the separation space to the outside is formed,

It is coupled to the second outlet side is extended to the lower portion of the separation space and characterized in that it comprises a guide tube for guiding the needle of the third syringe.

In addition, in the device of the present invention, the inlet, the first outlet, the second outlet is characterized in that the first, second, third rubber stopper of elastic to which the needle (needle) of each of the first, second, third syringe can enter It is done.

In the device of the present invention, the guide tube is composed of a large diameter portion coupled to the third rubber stopper, a small diameter portion eccentrically extending with the large diameter portion, and an inclined portion connecting the large diameter portion and the small diameter portion,

An incision portion through which an end of the needle of the third syringe passes is formed at a lower end of the small diameter portion.

In the device of the present invention, it is coupled to the lower portion of the body tube to prevent the separation of the slider, the center further comprises a lower cap formed with a through hole to enable the entrance and exit of the lifting mechanism for pushing up the slider upwards It is done.

In addition, the upper cap is characterized in that it further comprises a separator plate protruding perpendicular to the bottom surface of the reservoir.

The present invention has the following effects.

First, the separating space portion and the receiving space portion is formed inside the body tube via the intermediate passage portion is formed, and when the upper cap is coupled to the storage portion communicating with the passage portion is disposed under the upper cap, in one container As a result, the separation layers of blood can be separated and extracted, thereby preventing loss during transport and significantly reducing work time.

Second, the present invention has the advantage that since the separation and extraction of blood in the first centrifugation process is made in one container, the convenience of the operation is increased and the efficiency of the operation is improved.

Third, although the depth of the separation space 104 accommodated the white blood cells is deep, the needle 31 of the third syringe 30 is guided to the guide tube 400, it is possible to stable recovery.

Fourth, the receiving space 102 and the separation space 104 is partitioned by the inclined portion 105a so that the lower portion of the separation space 104 toward the inner wall surface side of the body tube 100 becomes narrower. In this case, the end of the needle 31 guided to the guide tube 400 is located at a position corresponding to the middle portion of the inclined portion 105a, so that the extraction of the white blood cells remaining in the lowermost portion of the separating space 104 gradually narrows. Although it becomes difficult, the end portion of the needle 31 is guided by the small diameter portion 402 and the incision portion 402a which are eccentrically positioned on the lower side of the guide tube 400 and remain at the bottom of the separation space 104. Enable the extraction of leukocytes.

1 is an exploded perspective view showing a blood separation and extraction device for platelet-rich growth factor extraction according to the present invention.

FIG. 2 is an exploded perspective view showing FIG. 1 from the bottom; FIG.

3 is a coupled state of FIG.

4 is a perspective view showing a coupling portion and a storage portion of the present invention.

Figures 5a, 5b, 5c, 5d, 5e, 5f, 5g is a use state diagram sequentially showing the use state of the present invention.

Figure 6 is a partially cut perspective view showing another embodiment of the present invention storage unit.

7 is a state diagram used in Figure 6,

8 is a cross-sectional view showing another embodiment of the device of the present invention;

9 is a perspective view of the guide tube of FIG. 8.

The present invention is centrifuged in a state in which the blood is accommodated in the receiving space portion formed in the lower portion of the body tube, and after separating the white blood cells, which are separation layers of the centrifuged blood, into the separation space portion of the body tube, the upper cap passage portion of the body tube. By coupling to communicate with each other so that only concentrated platelets can be collected and extracted, one centrifugation and separation of blood in one container and the extraction of concentrated platelets and leukocytes can proceed.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Blood separation and extraction device for platelet-rich growth factor extraction according to the present invention, when described with reference to Figs. 1 to 5, the receiving space 102 is formed in the lower portion receiving the blood, the receiving space portion A passage portion 105 having an inclined portion 105a and an upper end thereof is formed at an upper portion of the upper portion 102 so as to have an inner diameter narrower than a lower portion thereof, and a portion of the separation layer of blood separated by centrifugation of the blood is formed in the passage portion. A body tube (100) formed with a separation space (104) to be received via (105); The slider 200 is slidably coupled to the receiving space 102 and pushes the separation layer of blood separated by the centrifugal separation to the separation space 104 through the passage 105. ; Removably coupled to the upper end of the body tube 100, the coupling portion 310 is formed to be coupled to the upper end of the passage portion 105 when the body tube 100 is coupled to the lower center, the coupling It is formed to extend to one side of the lower portion 310, the upper cap 300 having a storage unit 320 is introduced into the concentration platelet (P) of the separation layer of blood when the slider 200 is raised; do.

The upper surface of the upper cap 300, the injection port (300c) that can be entered into the first syringe 10 for injecting the blood through the passage portion 105, and the concentrated platelets (received in the storage 320) ( The first outlet (300a) that can enter the second syringe 20 to extract the P and the outside of the third syringe 30 to extract the leukocytes (W) accommodated in the separation space 104 to the outside The second outlet 300b which is accessible is formed.

A guide tube 400 extending to the lower portion of the separation space 104 and guided by the needle 31 of the third syringe 30 is coupled to the second outlet 300b.

An elastic first to which needles of the first, second, and third syringes 10, 20, and 30 respectively enter the injection hole 300c, the first outlet 300a, and the second outlet 300b. 2, 3 rubber stoppers 304, 302, 303 are combined.

In more detail, the separation space 104 is a structure partitioned through the passage portion 105 from the accommodation space 102 on the upper side of the body tube 100, the blood separated by the centrifugation of the blood A portion of the separation layer is formed to be received and received through the passage portion 105 when the slider 200 is raised.

In addition, the lower portion of the body tube 100 to prevent the departure of the slider 200, the lower portion formed with a through hole 255 to allow the entrance and exit of the lifting tool (not shown) for pushing up the slider 200 upwards. The cap 250 is detachably coupled.

The passage part 105 is formed perpendicular to the upper side of the accommodation space 102, and has an inclined portion 105a to have an inner diameter that is narrower than the internal diameter of the accommodation space 102.

Accordingly, the receiving space 102 and the separating space 104 is partitioned by the inclined portion 105a so that the lower portion of the separating space 104 is received toward the inner wall surface of the body tube 100 toward the white blood cell (W) The space becomes narrower.

Coupling portion 310 of the upper cap 300 is formed with a coupling hole 312 having a lower opening to be fitted to the outer peripheral surface of the upper end of the passage portion 105.

The storage unit 320 has a structure formed such that the lower bottom surface is positioned at a lower position than the lower end of the coupling unit 310.

That is, the storage unit 320 has a structure in which the concentrated platelets P introduced through the passage unit 105 are accommodated.

The slider 200 is composed of first and second closure members 210 and 220.

The first closure member 210 maintains hermeticity around the outer circumferential surface, and a plurality of ring-shaped sealing members are coupled to reduce the volume of the accommodation space 102 at a uniform pressure during the ascending operation.

The second plug member 220 may be bonded to the lower portion of the first plug member 210 as a silicon material to protect the first plug member 210 during the lifting operation of the first plug member 210 by the elevating tool. Make sure

The first stopper member 210 is moved up by a lifting force transmitted from a separate lifting tool (actuator or human finger, such as a cylinder) to be raised along the inner circumferential surface of the receiving space 102 of the body tube 100. Have.

At this time, the elevating tool may enter the interior of the receiving space 102 through the through hole 255 of the lower cap 250 may impart a rising pressure to the slider 200.

As the height of the slider 200 increases, the extraction amount (eg, 1.0 to 2.5 cc) of the concentrated platelets P may change.

The concentrated platelets P extracted in the storage unit 320 may be extracted to the outside through the second syringe 20 after the predetermined volume of the platelets P is collected.

On the other hand, the storage unit 320 and the coupling portion 310 may be integrally formed on the lower surface of the upper cap 300 in one body, or may be detachably coupled to the lower portion of the upper cap 300.

8 and 9 show another embodiment of the inventive device.

This shows a modification of the guide tube 400, the large diameter portion 401 coupled to the third rubber stopper 303, the small diameter portion 402 and eccentrically extended with the large diameter portion 401 and And an inclined portion 403 connecting the large diameter portion 401 and the small diameter portion 402, and an end portion of the needle 31 of the third syringe 30 passes through a lower end portion of the small diameter portion 402. It has a structure in which the cut portion 402a is formed.

Referring to the operation of the present invention having such a configuration as follows.

In the blood separation and extraction apparatus for platelet-rich growth factor extraction according to the present invention, first referring to FIG. 5A, the first injection syringe 10 containing the blood is passed through the entrance port 304 and then the passage portion 105 is opened. Blood is injected into the receiving space 102 of the body tube 100, and the blood injected into the receiving space 102 using a centrifuge is centrifuged as shown in FIG. 5B.

The injection amount of blood can be injected about 30 ~ 50cc.

At this time, the upper cap 300 is maintained in a state coupled to the upper portion of the body tube 100 so that the separation space 104 is sealed during the centrifugal separation process.

When the centrifugal separation is completed, as shown in FIG. 5C, the coupling part 310 and the passage part 105 are moved upwardly apart from each other before the lifting operation of the slider 200.

When the blood is centrifuged, red blood cells (R), leukocytes (W), and platelets (P) are divided into cells, and white blood cells (W), platelets (P), and red blood cells (R) from the upper side according to the specific gravity difference. Are separated in order.

Subsequently, when the lifting tool enters the inside of the through hole 255 of the lower cap 250 and pushes the slider 200 upward, the slider 200 is lifted up inside the receiving space 102 while the blood separation layer is formed. The volume in the accommodation space 102 accommodated is reduced.

The slider 200 causes the leukocyte (W) layer of the separation layers of blood stored in the accommodation space 102 to be injected into the separation space 104 of the body tube 100 through the passage 105 when the slider 200 rises. .

The upward movement of the slider 200 is raised until the injection of the white blood cells (W) into the separation space 104 is completed.

At this time, the concentrated platelet (P) is located in the passage portion 105, the red blood cells (R) is maintained in the state accommodated in the lower portion of the receiving space (102).

Subsequently, as shown in FIG. 5D, when the upper cap 300 is lowered again to engage the coupling part 310 to be fitted to the end of the passage part 105, the inside and the upper portion of the receiving space 102 The storage unit 320 of the cap 300 is coupled to communicate with each other.

As shown in FIG. 5E, after the coupling part 310 of the upper cap 300 is fitted into the passage part 105, when the operator raises the slider 200 secondly using the lifting tool, a part of the separation layer of blood As the volume of the containing space portion 102 is further reduced is concentrated platelet (P) is introduced into the storage 320 of the upper cap 300 through the upper end of the passage portion 105.

The secondary ascending operation of the slider 200 is performed until the concentrated platelets P are introduced into the storage unit 320.

The capacity of the concentrated platelets (P) stored in the storage unit 320 varies depending on the amount of blood injected and the traits of the blood, and approximately 1.5 to 3.0 cc are concentrated and stored.

When the introduction of the concentrated platelets P into the storage unit 320 is completed, as shown in FIG. 5F, the operator passes the second syringe 20 through the second rubber stopper 302 of the upper cap 300 and stores it. Extracting the concentrated platelets (PRP) stored in the unit 320 to the outside.

Subsequently, as shown in FIG. 5G, the third syringe 30 passes through the third rubber stopper 303 of the upper cap 300 to extract the leukocytes W stored in the separation space 104 to the outside. Do this. At this time, the needle 31 of the third syringe 30 is guided to the guide tube 400 to be stably guided to the deep receiving space 104.

6 and 7 are views illustrating another embodiment of the storage unit of the present invention.

Another embodiment of the present invention storage unit is the same as the components of the above-described line embodiment, but is formed to protrude upwardly perpendicularly to the bottom surface of the storage unit 320 so that the inside of the storage unit 320 on the left and right in the drawing It is further provided with a separator plate 330 for partitioning.

Separation plate 330 is separated even if the concentrated platelet (P) and the red blood cells (R) is not separated into the storage unit 320 without being separated due to the misoperation caused by excessive operation of the slider 200, separation of the mixed blood The layers are separated from each other by using a specific gravity difference in the partitioned storage 320.

When the slider 200 excessively rises, the concentrated platelets P and the red blood cells R contained in the receiving space 102 may pass through the passage 105 and enter the storage 320.

In this case, even if the concentrated platelets (P) and red blood cells (R) are mixed into the storage unit 320 partitioned into the left and right spaces by the separator 330, the difference in specific gravity of the concentrated platelets (P) and red blood cells (R). It is separated in the storage unit 320 on the left and right by.

That is, when erythrocytes R are introduced after the light plated plate P, which is relatively lighter than the erythrocytes R, is introduced into the right storage unit 320 of the separator 330, the platelets 330 are over the top of the separator 330. It flows into the storage unit 320 on the left side.

The enriched platelets P are separated in the storage unit 320 on the left side based on the separator plate 330 and the red blood cells R are maintained in the right storage unit 320.

Thereafter, the process of extracting the separated platelets (P) separated using an extraction tool such as a syringe is the same as the process described above.

On the other hand, referring to Figure 5g, the receiving space 102 and the separation space 104 is partitioned by the inclined portion 105a so that the lower portion of the separation space 104 toward the inner wall surface side of the body tube 100 The space becomes narrow.

In this case, the end portion of the needle 31 guided to the guide tube 400 is located at a position corresponding to the middle portion of the inclined portion 105a as shown in FIG. 5G and remains at the bottom of the separating space 104 that gradually narrows. It is difficult to take out white blood cells.

8 and 9 showing another embodiment of the present invention, the device of this embodiment is a needle 31 by a small diameter portion 402 and an incision 402a which are eccentrically positioned on the lower side of the guide tube 400. The end of the) is guided to the inner wall surface side of the body tube 100 to enable extraction of the white blood cells remaining at the bottom of the separation space 104.

Due to this, the blood separation and extraction device for platelet-rich growth factor extraction of the present invention occurs in the existing transfer process because the separation and concentration operation through centrifugation of blood is performed through one container and one centrifugation process. In addition to preventing the loss, and has the effect of increasing the convenience of work.

The present invention relates to a blood separation and extraction device for platelet-rich growth factor extraction, in particular to allow the separation and concentration operation can be carried out in one container at the same time, and to perform the separation and extraction by only the first centrifugation The structure is improved.

Claims (5)

An accommodating space portion 102 is formed at a lower portion thereof, and an inclined portion 105a is formed at an upper portion of the accommodating space portion 102 so that an inner diameter thereof is narrower than a lower portion thereof, and a passage portion 105 having an upper end thereof is formed. A body tube (100) having a separation space (104) in which a portion of the separation layer of blood separated by the centrifugation of blood is received via the passage portion (105); The slider 200 is slidably coupled to the receiving space 102 and pushes the separation layer of blood separated by the centrifugal separation to the separation space 104 through the passage 105. ; Removably coupled to the upper end of the body tube 100, the coupling portion 310 is formed to be coupled to the upper end of the passage portion 105 when the body tube 100 is coupled to the lower center, the coupling It is formed to extend to one side of the lower portion 310, the upper cap 300 having a storage unit 320 is introduced to receive the concentrated platelets (P) in the separation layer of blood when the slider 200 is raised; and, The upper cap 300 is an injection port 300c through which the first syringe 10 for injecting the blood through the passage part 105 is provided on the upper surface thereof, and the concentrated platelets accommodated in the storage part 320 ( The first outlet (300a) that can enter the second syringe 20 to extract the P and the outside of the third syringe 30 to extract the leukocytes (W) accommodated in the separation space 104 to the outside A second outlet 300b which is accessible is formed, It is coupled to the second outlet (300b) side and extends to the lower portion of the separation space 104, characterized in that it comprises a guide tube 400 to guide the needle 31 of the third syringe (30) Blood separation and extraction device for extracting platelet-rich growth factor. The needle of each of the first, second, and third syringes 10, 20, 30 enters the injection hole 300c, the first outlet 300a, and the second outlet 300b. Blood separation and extraction device for platelet-rich growth factor extraction, characterized in that the first, second, third rubber stoppers (304, 302, 303) of the elasticity possible. The method of claim 2, wherein the guide tube 400 is a large diameter portion 401 coupled to the third rubber stopper 303, a small diameter portion 402 extending eccentrically with the large diameter portion 401, the table Is made up of the inclined portion 403 connecting the neck portion 401 and the small diameter portion 402, Blood separation and extraction device for platelet-rich growth factor extraction, characterized in that the incision 402a is formed at the lower end of the small diameter portion 402, the end portion of the needle 31 of the third syringe 30 is passed through . The method according to claim 1, It is coupled to the lower portion of the body tube 100 to prevent the separation of the slider 200, the through hole 255 to allow the entrance and exit of the lifting mechanism to push the slider 200 upward in the center Blood separation and extraction device for platelet-rich growth factor extraction, characterized in that it further comprises a formed lower cap 250. The method according to claim 1, The upper cap 300 is a blood separation and extraction device for platelet-rich growth factor extraction, characterized in that further comprising a separator plate 330 is formed to protrude perpendicularly to the bottom surface of the storage unit 320.

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