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WO2016060537A3 - Sondes et amorces pour la quantification rapide et fiable des gènes spécifiques au cancer de la prostate en utilisant des gènes contrôles adéquats - Google Patents

Sondes et amorces pour la quantification rapide et fiable des gènes spécifiques au cancer de la prostate en utilisant des gènes contrôles adéquats Download PDF

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Publication number
WO2016060537A3
WO2016060537A3 PCT/MA2015/000022 MA2015000022W WO2016060537A3 WO 2016060537 A3 WO2016060537 A3 WO 2016060537A3 MA 2015000022 W MA2015000022 W MA 2015000022W WO 2016060537 A3 WO2016060537 A3 WO 2016060537A3
Authority
WO
WIPO (PCT)
Prior art keywords
genes
primers
probes
rapid
prostate cancer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/MA2015/000022
Other languages
English (en)
Other versions
WO2016060537A2 (fr
Inventor
Abdeladim MOUMEN
Imane ABDELLAOUI-MAANE
El Hassane SEFRIOUI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Moroccan Foundation For Advanced Science Innovation & Research (mascir)
Original Assignee
Moroccan Foundation For Advanced Science Innovation & Research (mascir)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Moroccan Foundation For Advanced Science Innovation & Research (mascir) filed Critical Moroccan Foundation For Advanced Science Innovation & Research (mascir)
Publication of WO2016060537A2 publication Critical patent/WO2016060537A2/fr
Publication of WO2016060537A3 publication Critical patent/WO2016060537A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne de nouveaux sets d'amorces et sondes et leur utilisation pour la détection des gènes spécifiques de la prostate à savoir les gènes PCA3, TMPRSS2- ERG et AMACR et des gènes de contrôles choisis parmi les gènes RPL5, HPRT1, et HNRNPA1 pour la normalisation des données d'analyse d'expression des gènes cibles à partir d'échantillons de patients que ce soit des échantillons tissulaires ou des fluides biologiques. Les sets d'amorces et sondes des gènes cibles et gènes de références sont plus sensibles que ceux citées en littérature et seront utilisées en PCR en temps réel (qPCR) en format simplex ou multiplex pour une quantification fiable, rapide et moins coûteuse.
PCT/MA2015/000022 2014-10-17 2015-12-16 Sondes et amorces pour la quantification rapide et fiable des gènes spécifiques au cancer de la prostate en utilisant des gènes contrôles adéquats Ceased WO2016060537A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MA37434 2014-10-17
MA37434A MA37434B1 (fr) 2014-10-17 2014-10-17 Sondes et amorces pour la quantification rapide et fiable des gènes spécifiques au cancer de la prostate en utilisant des gènes controles adéquats

Publications (2)

Publication Number Publication Date
WO2016060537A2 WO2016060537A2 (fr) 2016-04-21
WO2016060537A3 true WO2016060537A3 (fr) 2016-09-29

Family

ID=55747527

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MA2015/000022 Ceased WO2016060537A2 (fr) 2014-10-17 2015-12-16 Sondes et amorces pour la quantification rapide et fiable des gènes spécifiques au cancer de la prostate en utilisant des gènes contrôles adéquats

Country Status (2)

Country Link
MA (1) MA37434B1 (fr)
WO (1) WO2016060537A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106636000A (zh) * 2016-12-23 2017-05-10 广东圣赛生物科技有限公司 hESCs‑TK细胞系及其构建方法和应用
CN110628908B (zh) * 2019-09-19 2022-05-10 北京恩泽康泰生物科技有限公司 用于前列腺癌诊断分级和良恶性预测的生物标志物及检测试剂盒
CN116064790A (zh) * 2022-08-10 2023-05-05 海南松林生物科技有限责任公司 用于前列腺癌筛查的标记物、试剂盒及其使用方法
CN116519936B (zh) * 2023-02-28 2025-11-07 杭州凯保罗生物科技有限公司 用于前列腺癌早期检测的肿瘤自身抗原/抗体组合及应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006032394A1 (de) * 2006-03-17 2007-09-20 Technische Universität Dresden Verfahren zur Diagnose und Differenzierung von Prostatakrebs

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006032394A1 (de) * 2006-03-17 2007-09-20 Technische Universität Dresden Verfahren zur Diagnose und Differenzierung von Prostatakrebs

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
FALK OHL ET AL: "Gene expression studies in prostate cancer tissue: which reference gene should be selected for normalization?", JOURNAL OF MOLECULAR MEDICINE, SPRINGER, BERLIN, DE, vol. 83, no. 12, 1 December 2005 (2005-12-01), pages 1014 - 1024, XP019320313, ISSN: 1432-1440, DOI: 10.1007/S00109-005-0703-Z *
IGOR TSAUR ET AL: "Reliable Housekeeping Gene Combination for Quantitative PCR of Lymph Nodes in Patients with Prostate Cancer", ANTICANCER RESEARCH - INTERNATIONAL JOURNAL OF CANCER RESEARCH AND TREATMENT, vol. 33, no. 12, 1 December 2013 (2013-12-01), GR, pages 5243 - 5248, XP055293910, ISSN: 0250-7005 *
REYNOLDS ET AL: "Molecular markers for prostate cancer", CANCER LETTERS, NEW YORK, NY, US, vol. 249, no. 1, 28 March 2007 (2007-03-28), pages 5 - 13, XP005929141, ISSN: 0304-3835, DOI: 10.1016/J.CANLET.2006.12.029 *
SCHMIDT UTA ET AL: "Quantitative multi-gene expression profiling of primary prostate cancer", PROSTATE, WILEY-LISS, NEW YORK, NY, US, vol. 66, no. 14, 1 October 2006 (2006-10-01), pages 1521 - 1534, XP002600867, ISSN: 0270-4137 *
SHARAN R N ET AL: "Consensus reference gene(s) for gene expression studies in human cancers: end of the tunnel visible?", CELLULAR ONCOLOGY, SPRINGER, DORDRECHT, vol. 38, no. 6, 18 September 2015 (2015-09-18), pages 419 - 431, XP035969706, ISSN: 2211-3428, [retrieved on 20150918], DOI: 10.1007/S13402-015-0244-6 *

Also Published As

Publication number Publication date
WO2016060537A2 (fr) 2016-04-21
MA37434B1 (fr) 2017-01-31
MA37434A1 (fr) 2016-05-31

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