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WO2015116969A3 - Method, vectors, cells, seeds and kits for stacking genes into a single genomic site - Google Patents

Method, vectors, cells, seeds and kits for stacking genes into a single genomic site Download PDF

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Publication number
WO2015116969A3
WO2015116969A3 PCT/US2015/013845 US2015013845W WO2015116969A3 WO 2015116969 A3 WO2015116969 A3 WO 2015116969A3 US 2015013845 W US2015013845 W US 2015013845W WO 2015116969 A3 WO2015116969 A3 WO 2015116969A3
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WO
WIPO (PCT)
Prior art keywords
methods
kits
vectors
seeds
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2015/013845
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French (fr)
Other versions
WO2015116969A2 (en
Inventor
Vibha Srivastava
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Arkansas at Fayetteville
University of Arkansas at Little Rock
Original Assignee
University of Arkansas at Fayetteville
University of Arkansas at Little Rock
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Filing date
Publication date
Application filed by University of Arkansas at Fayetteville, University of Arkansas at Little Rock filed Critical University of Arkansas at Fayetteville
Priority to US15/114,724 priority Critical patent/US10233456B2/en
Publication of WO2015116969A2 publication Critical patent/WO2015116969A2/en
Publication of WO2015116969A3 publication Critical patent/WO2015116969A3/en
Anticipated expiration legal-status Critical
Priority to US16/290,573 priority patent/US11066676B2/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Methods of gene stacking are described herein. The methods can be used to repeatedly add genes into a chosen locus in a precise manner, which ensures co-segregation of all introduced genes and contributes to the stabilization of gene expression. In addition, methods of removing any additional foreign DNA elements such as selectable markers are provided. Seed stocks or cell lines comprising a gene stacking site, vectors containing an insert flanked by target sites for a site-specific DNA recombinase for use in the methods and kits for carrying out the methods are also provided herein.
PCT/US2015/013845 2014-01-30 2015-01-30 Method, vectors, cells, seeds and kits for stacking genes into a single genomic site Ceased WO2015116969A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US15/114,724 US10233456B2 (en) 2014-01-30 2015-01-30 Method, vectors, cells, seeds and kits for stacking genes into a single genomic site
US16/290,573 US11066676B2 (en) 2014-01-30 2019-03-01 Method, vectors, cells, seeds and kits for stacking genes into a single genomic site

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461933646P 2014-01-30 2014-01-30
US61/933,646 2014-01-30

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US15/114,724 A-371-Of-International US10233456B2 (en) 2014-01-30 2015-01-30 Method, vectors, cells, seeds and kits for stacking genes into a single genomic site
US16/290,573 Division US11066676B2 (en) 2014-01-30 2019-03-01 Method, vectors, cells, seeds and kits for stacking genes into a single genomic site

Publications (2)

Publication Number Publication Date
WO2015116969A2 WO2015116969A2 (en) 2015-08-06
WO2015116969A3 true WO2015116969A3 (en) 2015-11-19

Family

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Family Applications (1)

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PCT/US2015/013845 Ceased WO2015116969A2 (en) 2014-01-30 2015-01-30 Method, vectors, cells, seeds and kits for stacking genes into a single genomic site

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US (2) US10233456B2 (en)
WO (1) WO2015116969A2 (en)

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EP3177718B1 (en) 2014-07-30 2022-03-16 President and Fellows of Harvard College Cas9 proteins including ligand-dependent inteins
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US10892034B2 (en) 2016-07-01 2021-01-12 Microsoft Technology Licensing, Llc Use of homology direct repair to record timing of a molecular event
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US20180004537A1 (en) 2016-07-01 2018-01-04 Microsoft Technology Licensing, Llc Molecular State Machines
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WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
KR20190123328A (en) 2017-03-09 2019-10-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Cancer vaccine
JP2020510439A (en) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ Base-editing factor from cytosine to guanine
KR102687373B1 (en) 2017-03-23 2024-07-23 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Nucleobase editing agent comprising a nucleic acid programmable DNA binding protein
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
EP3658573A1 (en) 2017-07-28 2020-06-03 President and Fellows of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
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US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
CN108384812B (en) * 2018-01-23 2019-10-18 华南理工大学 A yeast genome editing vector and its construction method and application
CN108165660A (en) * 2018-03-16 2018-06-15 深圳盛宝联合谷物股份有限公司 The molecular labeling and its screening technique of QTL close linkages related to rice meal taste
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JP2021530968A (en) 2018-05-24 2021-11-18 モンサント テクノロジー エルエルシー Genome editing in plants
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Also Published As

Publication number Publication date
US10233456B2 (en) 2019-03-19
US20160333363A1 (en) 2016-11-17
US20190323021A1 (en) 2019-10-24
WO2015116969A2 (en) 2015-08-06
US11066676B2 (en) 2021-07-20

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