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WO2015006811A3 - Diagnostic methods - Google Patents

Diagnostic methods Download PDF

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Publication number
WO2015006811A3
WO2015006811A3 PCT/AU2014/000730 AU2014000730W WO2015006811A3 WO 2015006811 A3 WO2015006811 A3 WO 2015006811A3 AU 2014000730 W AU2014000730 W AU 2014000730W WO 2015006811 A3 WO2015006811 A3 WO 2015006811A3
Authority
WO
WIPO (PCT)
Prior art keywords
genetic
test
sample
concordance
degree
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/AU2014/000730
Other languages
French (fr)
Other versions
WO2015006811A2 (en
Inventor
Marnie BLEWITT
Leonard Charles Harrison
Jovana MAKSIMOVIC
Gaetano NASELLI
Alicia OSHLACK
Yuxia Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Walter and Eliza Hall Institute of Medical Research
Murdoch Childrens Research Institute
Original Assignee
Walter and Eliza Hall Institute of Medical Research
Murdoch Childrens Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2013902753A external-priority patent/AU2013902753A0/en
Application filed by Walter and Eliza Hall Institute of Medical Research, Murdoch Childrens Research Institute filed Critical Walter and Eliza Hall Institute of Medical Research
Publication of WO2015006811A2 publication Critical patent/WO2015006811A2/en
Anticipated expiration legal-status Critical
Publication of WO2015006811A3 publication Critical patent/WO2015006811A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for identifying the level of T-regulatory cell activity in a test biological sample comprising immune cells, the method comprising screening a test sample for the methylation profiles of a pre-selected genetic locus/loci selected from the list consisting of: (i) at least one genetic locus or at least two genetic Loci defined m one or more of Table 1 to Table 5; (ii) at least one genetic locus or at least two genetic loci defined in one or more of Table 6 to Table 10; and (iii) at least two genetic loci defined in one or more of Table 1 to Table 5, and one or more of Table 6 to Table 10; and determining the degree of concordance between the test methylation profiles and a reference methylation profile, wherein the degree of concordance identifies the level of T-regulatory cell activity in the sample.
PCT/AU2014/000730 2013-07-19 2014-07-18 Diagnostic methods Ceased WO2015006811A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2013902753A AU2013902753A0 (en) 2013-07-19 Diagnostic Methods
AU2013902753 2013-07-19

Publications (2)

Publication Number Publication Date
WO2015006811A2 WO2015006811A2 (en) 2015-01-22
WO2015006811A3 true WO2015006811A3 (en) 2016-06-23

Family

ID=52346782

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2014/000730 Ceased WO2015006811A2 (en) 2013-07-19 2014-07-18 Diagnostic methods

Country Status (1)

Country Link
WO (1) WO2015006811A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016094961A1 (en) * 2014-12-19 2016-06-23 Murdoch Childrens Research Institute Methylation assay
CA3041055A1 (en) * 2016-11-04 2018-05-11 Qiagen Sciences, Llc Thiol-containing cleave reagents and oxidative wash

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1826278A1 (en) * 2006-02-28 2007-08-29 Epiontis GmbH Epigenetic modification of the loci for camta1 and/or foxp3 as a marker for cancer treatment
WO2009126688A2 (en) * 2008-04-09 2009-10-15 Genentech, Inc. Novel compositions and methods for the treatment of immune related diseases

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1826278A1 (en) * 2006-02-28 2007-08-29 Epiontis GmbH Epigenetic modification of the loci for camta1 and/or foxp3 as a marker for cancer treatment
EP1826279A1 (en) * 2006-02-28 2007-08-29 Charite-Universitätsmedizin Berlin Detection and quality control of regulatory T cells through DNA-methylation analysis of the FoxP3 gene
WO2009126688A2 (en) * 2008-04-09 2009-10-15 Genentech, Inc. Novel compositions and methods for the treatment of immune related diseases

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BARON, U. ET AL.: "DNA demethylation in the human FOXP3 locus discriminates regulatory T cells from activated FOXP3+ conventional T cells", EUROPEAN JOURNAL OF IMMUNOLOGY, vol. 37, 2007, pages 2378 - 2389, XP002503237, DOI: doi:10.1002/EJI.200737594 *
OKHURA, N. ET AL.: "T Cell Receptor Stimulation-Induced Epigenetic Changes and Foxp3 Expression Are Independent and Complementary Events Required for Treg Cell Development", IMMUNITY, vol. 37, 2012, pages 785 - 799 *
ROUAS, R. ET AL.: "Human natural Treg microRNA signature: Role of microRNA-31 and microRNA-21 in FOXP3 expression", EUROPEAN JOURNAL OF IMMUNOLOGY, vol. 39, 2009, pages 1608 - 1618 *
SCHMIDL, C. ET AL.: "Lineage-specific DNA methylation in T cells correlates with histone methylation and enhancer activity", GENOME RESEARCH, vol. 19, 2009, pages 1165 - 1174 *
ZHANG, Y. ET AL.: "Genome-wide DNA methylation analysis identifies hypomethylated genes regulated by FOXP3 in human regulatory T cells", BLOOD, vol. 122, no. 16, 13 October 2013 (2013-10-13), pages 2823 - 2836 *

Also Published As

Publication number Publication date
WO2015006811A2 (en) 2015-01-22

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