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WO2014203269A3 - Procédé d'isolement, de purification et d'expansion à l'échelle industrielle de cellules souches mésenchymateuses dérivées de tissu adipeux canin - Google Patents

Procédé d'isolement, de purification et d'expansion à l'échelle industrielle de cellules souches mésenchymateuses dérivées de tissu adipeux canin Download PDF

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Publication number
WO2014203269A3
WO2014203269A3 PCT/IN2013/000584 IN2013000584W WO2014203269A3 WO 2014203269 A3 WO2014203269 A3 WO 2014203269A3 IN 2013000584 W IN2013000584 W IN 2013000584W WO 2014203269 A3 WO2014203269 A3 WO 2014203269A3
Authority
WO
WIPO (PCT)
Prior art keywords
isolation
purification
stem cells
mesenchymal stem
adipose tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IN2013/000584
Other languages
English (en)
Other versions
WO2014203269A2 (fr
Inventor
Satish Mahadeorao Totey
Manoj Kumar C REDDY
Lyle Carl FONSECA
Aarya HARI
Basavaraj CHOUGULE
Minita SODHI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KASIAK RESEARCH PVT Ltd
Original Assignee
KASIAK RESEARCH PVT Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KASIAK RESEARCH PVT Ltd filed Critical KASIAK RESEARCH PVT Ltd
Publication of WO2014203269A2 publication Critical patent/WO2014203269A2/fr
Publication of WO2014203269A3 publication Critical patent/WO2014203269A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Virology (AREA)
  • Rheumatology (AREA)
  • Epidemiology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé d'isolement, de purification et d'expansion à l'échelle industrielle de cellules souches mésenchymateuses (MSC) dérivées de tissu adipeux canin. L'invention concerne également un procédé de traitement et un produit thérapeutique pour traiter l'arthrose, une lésion de la moelle épinière, la dermatite atopique, une cardiomyopathie dilatée, le diabète de type 1, l'insuffisance rénale, une maladie hépatique et des plaies ne cicatrisant pas, comprenant des MSC.
PCT/IN2013/000584 2013-06-17 2013-09-26 Procédé d'isolement, de purification et d'expansion à l'échelle industrielle de cellules souches mésenchymateuses dérivées de tissu adipeux canin Ceased WO2014203269A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2046/MUM/2013 2013-06-17
IN2046MU2013 IN2013MU02046A (fr) 2013-06-17 2013-09-26

Publications (2)

Publication Number Publication Date
WO2014203269A2 WO2014203269A2 (fr) 2014-12-24
WO2014203269A3 true WO2014203269A3 (fr) 2015-03-26

Family

ID=52105424

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2013/000584 Ceased WO2014203269A2 (fr) 2013-06-17 2013-09-26 Procédé d'isolement, de purification et d'expansion à l'échelle industrielle de cellules souches mésenchymateuses dérivées de tissu adipeux canin

Country Status (2)

Country Link
IN (1) IN2013MU02046A (fr)
WO (1) WO2014203269A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
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CN105132500A (zh) * 2015-08-31 2015-12-09 李青峰 一种svf的制备方法

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HUP1500218A2 (hu) * 2015-05-08 2016-11-28 Deltabio 2000 Kft Eljárás és készítmény ortopédiai betegségek, így ízületiporc-sérülések, különösen ízületi diszplázia kezelésére
CN105238748A (zh) * 2015-10-27 2016-01-13 上海百众源生物科技有限公司 一种胎盘源壁蜕膜间充质干细胞分离冷冻的制备方法
CN105685015B (zh) * 2016-03-10 2018-11-27 广州赛莱拉干细胞科技股份有限公司 一种细胞冻存液
CN107746829B (zh) * 2017-10-19 2022-03-22 佛山科学技术学院 一种分离与原代培养犬胎盘来源的间充质干细胞的方法
EP3720948A1 (fr) 2018-03-12 2020-10-14 Universidade do Porto Compositions destinées à être utilisées dans le traitement d'affections musculo-squelettiques et méthodes de production de celles-ci tirant profit de l'activité synergique de deux types différents de cellules souches/stromales mésenchymateuses
EP3806872A1 (fr) * 2018-06-15 2021-04-21 Mariñas Pardo, Luis Composition pharmaceutique pour dermatologie et ses utilisations
CN111621469A (zh) * 2020-06-01 2020-09-04 重庆医科大学 一种改良酶消化法分离骨细胞的技术及其应用
HU231475B1 (hu) * 2021-04-01 2024-02-28 Cellfactory Korlátolt Felelősségű Társaság Eljárás osteoartritisben szenvedő egyed kezelésére szolgáló készítmény előállítására
AU2022308332A1 (en) * 2021-07-08 2024-01-18 Boehringer Ingelheim Veterinary Medicine Belgium Mesenchymal stem cells for use in the treatment of atopic dermatitis
CN113774020B (zh) * 2021-08-24 2023-04-14 河北朋和生物科技有限公司 一种脂肪间充质干细胞库的构建方法
BR102022016233A2 (pt) * 2022-08-16 2024-02-27 Omics Biotecnologia Animal Ltda Concentrado de proteínas e peptídeos derivados de células estromais mesenquimais, método de obtenção e uso de concentrado

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110081323A1 (en) * 2005-09-21 2011-04-07 Dask Technologies, Llc Methods and compositions for organ and tissue functionality
US20110229965A1 (en) * 2009-11-27 2011-09-22 Rakhi Pal Methods of Preparing Mesenchymal Stem Cells, Compositions and Kit Thereof
US20110244566A1 (en) * 2010-02-01 2011-10-06 Joseph Wu Enhanced efficiency of induced pluripotent stem cell generation
US20120244130A1 (en) * 2009-10-06 2012-09-27 National University Corporation Nagoya University Cell preparation for erectile dysfunction or sensory disorders of the lower urinary tract containing adipose tissue derived mesenchymal stem cells
US20130129688A1 (en) * 2011-11-09 2013-05-23 Allocure, Inc. Assay for the Prediction of Therapeutic Effectiveness or Potency of Mesenchymal Stem Cells, and Methods of Using Same

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110081323A1 (en) * 2005-09-21 2011-04-07 Dask Technologies, Llc Methods and compositions for organ and tissue functionality
US20120244130A1 (en) * 2009-10-06 2012-09-27 National University Corporation Nagoya University Cell preparation for erectile dysfunction or sensory disorders of the lower urinary tract containing adipose tissue derived mesenchymal stem cells
US20110229965A1 (en) * 2009-11-27 2011-09-22 Rakhi Pal Methods of Preparing Mesenchymal Stem Cells, Compositions and Kit Thereof
US20110244566A1 (en) * 2010-02-01 2011-10-06 Joseph Wu Enhanced efficiency of induced pluripotent stem cell generation
US20130129688A1 (en) * 2011-11-09 2013-05-23 Allocure, Inc. Assay for the Prediction of Therapeutic Effectiveness or Potency of Mesenchymal Stem Cells, and Methods of Using Same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CARVALHO ET AL.: "Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue.", VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, vol. 132.2, 2009, pages 303 - 306 *
OEDAYRAJSINGH-VARMA, M. J. ET AL., CYTOTHERAPY, vol. 8.2, 2006, pages 166 - 177 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105132500A (zh) * 2015-08-31 2015-12-09 李青峰 一种svf的制备方法

Also Published As

Publication number Publication date
IN2013MU02046A (fr) 2015-06-05
WO2014203269A2 (fr) 2014-12-24

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