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WO2014203269A3 - Method for isolation, purification and industrial scale expansion of canine adipose tissue derived mesenchymal stem cells - Google Patents

Method for isolation, purification and industrial scale expansion of canine adipose tissue derived mesenchymal stem cells Download PDF

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Publication number
WO2014203269A3
WO2014203269A3 PCT/IN2013/000584 IN2013000584W WO2014203269A3 WO 2014203269 A3 WO2014203269 A3 WO 2014203269A3 IN 2013000584 W IN2013000584 W IN 2013000584W WO 2014203269 A3 WO2014203269 A3 WO 2014203269A3
Authority
WO
WIPO (PCT)
Prior art keywords
isolation
purification
stem cells
mesenchymal stem
adipose tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IN2013/000584
Other languages
French (fr)
Other versions
WO2014203269A2 (en
Inventor
Satish Mahadeorao Totey
Manoj Kumar C REDDY
Lyle Carl FONSECA
Aarya HARI
Basavaraj CHOUGULE
Minita SODHI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KASIAK RESEARCH PVT Ltd
Original Assignee
KASIAK RESEARCH PVT Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KASIAK RESEARCH PVT Ltd filed Critical KASIAK RESEARCH PVT Ltd
Publication of WO2014203269A2 publication Critical patent/WO2014203269A2/en
Publication of WO2014203269A3 publication Critical patent/WO2014203269A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Virology (AREA)
  • Rheumatology (AREA)
  • Epidemiology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for isolation, purification and industrial scale expansion of canine adipose tissue derived mesenchymal stem cells (MSCs). The invention also relates to a method for treating and a therapeutic product for treating osteoarthritis, spinal cord injury, atopic dermatitis, dilated cardiomyopathy, type-1 diabetes, renal failure, hepatic disease and non healing wounds comprising MSCs.
PCT/IN2013/000584 2013-06-17 2013-09-26 Method for isolation, purification and industrial scale expansion of canine adipose tissue derived mesenchymal stem cells Ceased WO2014203269A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2046/MUM/2013 2013-06-17
IN2046MU2013 IN2013MU02046A (en) 2013-06-17 2013-09-26

Publications (2)

Publication Number Publication Date
WO2014203269A2 WO2014203269A2 (en) 2014-12-24
WO2014203269A3 true WO2014203269A3 (en) 2015-03-26

Family

ID=52105424

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2013/000584 Ceased WO2014203269A2 (en) 2013-06-17 2013-09-26 Method for isolation, purification and industrial scale expansion of canine adipose tissue derived mesenchymal stem cells

Country Status (2)

Country Link
IN (1) IN2013MU02046A (en)
WO (1) WO2014203269A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105132500A (en) * 2015-08-31 2015-12-09 李青峰 SVF (stromal vascular fraction) preparation method

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUP1500218A2 (en) * 2015-05-08 2016-11-28 Deltabio 2000 Kft Method and composition for treatment of orthopedic diseases articular cortilage injuries and particularly to dysphoria
CN105238748A (en) * 2015-10-27 2016-01-13 上海百众源生物科技有限公司 Preparation method of placenta-source decidua parietalis mesenchymal stem cells by separation and refrigeration
CN105685015B (en) * 2016-03-10 2018-11-27 广州赛莱拉干细胞科技股份有限公司 a cell freezing medium
CN107746829B (en) * 2017-10-19 2022-03-22 佛山科学技术学院 Method for separating and primary culturing dog placenta-derived mesenchymal stem cells
US20210047621A1 (en) 2018-03-12 2021-02-18 Universidade Do Porto Compositions for use in the treatment of musculoskeletal conditions and methods for producing the same leveraging the synergistic activity of two different types of mesenchymal stromal/stem cells
MA52883A (en) * 2018-06-15 2021-05-26 Prieto Manuel Hermida PHARMACEUTICAL COMPOSITION FOR DERMATOLOGY AND ITS USES
CN111621469A (en) * 2020-06-01 2020-09-04 重庆医科大学 Technology for separating osteocyte by improved enzyme digestion method and application thereof
HU231475B1 (en) * 2021-04-01 2024-02-28 Cellfactory Korlátolt Felelősségű Társaság Process for the production of a composition for the treatment of an individual suffering from osteoarthritis
WO2023280834A1 (en) * 2021-07-08 2023-01-12 Boehringer Ingelheim Veterinary Medicine Belgium Mesenchymal stem cells for use in the treatment of atopic dermatitis
CN113774020B (en) * 2021-08-24 2023-04-14 河北朋和生物科技有限公司 A method for constructing adipose-derived mesenchymal stem cell bank
BR102022016233A2 (en) * 2022-08-16 2024-02-27 Omics Biotecnologia Animal Ltda CONCENTRATE OF PROTEINS AND PEPTIDES DERIVED FROM MESENCHYMAL STROMA CELLS, METHOD OF OBTAINING AND USE OF CONCENTRATE

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110081323A1 (en) * 2005-09-21 2011-04-07 Dask Technologies, Llc Methods and compositions for organ and tissue functionality
US20110229965A1 (en) * 2009-11-27 2011-09-22 Rakhi Pal Methods of Preparing Mesenchymal Stem Cells, Compositions and Kit Thereof
US20110244566A1 (en) * 2010-02-01 2011-10-06 Joseph Wu Enhanced efficiency of induced pluripotent stem cell generation
US20120244130A1 (en) * 2009-10-06 2012-09-27 National University Corporation Nagoya University Cell preparation for erectile dysfunction or sensory disorders of the lower urinary tract containing adipose tissue derived mesenchymal stem cells
US20130129688A1 (en) * 2011-11-09 2013-05-23 Allocure, Inc. Assay for the Prediction of Therapeutic Effectiveness or Potency of Mesenchymal Stem Cells, and Methods of Using Same

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110081323A1 (en) * 2005-09-21 2011-04-07 Dask Technologies, Llc Methods and compositions for organ and tissue functionality
US20120244130A1 (en) * 2009-10-06 2012-09-27 National University Corporation Nagoya University Cell preparation for erectile dysfunction or sensory disorders of the lower urinary tract containing adipose tissue derived mesenchymal stem cells
US20110229965A1 (en) * 2009-11-27 2011-09-22 Rakhi Pal Methods of Preparing Mesenchymal Stem Cells, Compositions and Kit Thereof
US20110244566A1 (en) * 2010-02-01 2011-10-06 Joseph Wu Enhanced efficiency of induced pluripotent stem cell generation
US20130129688A1 (en) * 2011-11-09 2013-05-23 Allocure, Inc. Assay for the Prediction of Therapeutic Effectiveness or Potency of Mesenchymal Stem Cells, and Methods of Using Same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CARVALHO ET AL.: "Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue.", VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, vol. 132.2, 2009, pages 303 - 306 *
OEDAYRAJSINGH-VARMA, M. J. ET AL., CYTOTHERAPY, vol. 8.2, 2006, pages 166 - 177 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105132500A (en) * 2015-08-31 2015-12-09 李青峰 SVF (stromal vascular fraction) preparation method

Also Published As

Publication number Publication date
IN2013MU02046A (en) 2015-06-05
WO2014203269A2 (en) 2014-12-24

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